Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014874.01.T01 | XP_024633681.1 | 97.196 | 107 | 3 | 0 | 1 | 107 | 128 | 234 | 1.42E-69 | 218 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014874.01.T01 | Q9FLE8 | 41.129 | 124 | 54 | 3 | 1 | 105 | 265 | 388 | 1.54E-19 | 84.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014874.01.T01 | A0A396ISP9 | 97.196 | 107 | 3 | 0 | 1 | 107 | 128 | 234 | 6.79e-70 | 218 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003493.01 | MsG0380014874.01 | 0.805933 | 1.050724e-49 | 5.630169e-47 |
| MsG0380014874.01 | MsG0580024463.01 | 0.810372 | 1.191338e-50 | 7.168483e-48 |
| MsG0380014874.01 | MsG0580027925.01 | 0.818221 | 2.195164e-52 | 1.633271e-49 |
| MsG0380014874.01 | MsG0780036136.01 | 0.807494 | 4.918206e-50 | 2.743428e-47 |
| MsG0380014874.01 | MsG0880046059.01 | 0.806820 | 6.832677e-50 | 3.745168e-47 |
| MsG0280006783.01 | MsG0380014874.01 | 0.805459 | 1.321238e-49 | 6.994054e-47 |
| MsG0280007955.01 | MsG0380014874.01 | 0.808944 | 2.414591e-50 | 1.399028e-47 |
| MsG0280010015.01 | MsG0380014874.01 | 0.805706 | 1.172831e-49 | 6.248240e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014874.01.T01 | MTR_3g465550 | 97.030 | 101 | 3 | 0 | 1 | 101 | 128 | 228 | 1.07e-68 | 205 |
| MsG0380014874.01.T01 | MTR_5g072400 | 63.810 | 105 | 37 | 1 | 1 | 105 | 150 | 253 | 3.83e-43 | 141 |
| MsG0380014874.01.T01 | MTR_5g083110 | 51.429 | 105 | 50 | 1 | 1 | 105 | 135 | 238 | 1.77e-31 | 111 |
| MsG0380014874.01.T01 | MTR_7g090230 | 48.598 | 107 | 53 | 2 | 1 | 105 | 318 | 424 | 2.47e-28 | 106 |
| MsG0380014874.01.T01 | MTR_3g017490 | 48.624 | 109 | 53 | 3 | 1 | 107 | 155 | 262 | 4.95e-28 | 103 |
| MsG0380014874.01.T01 | MTR_1g051180 | 46.552 | 116 | 50 | 3 | 1 | 105 | 249 | 363 | 1.13e-27 | 103 |
| MsG0380014874.01.T01 | MTR_3g071510 | 42.857 | 105 | 59 | 1 | 1 | 105 | 131 | 234 | 4.64e-26 | 97.4 |
| MsG0380014874.01.T01 | MTR_1g090400 | 50.588 | 85 | 41 | 1 | 22 | 105 | 284 | 368 | 1.02e-23 | 93.2 |
| MsG0380014874.01.T01 | MTR_8g038440 | 45.872 | 109 | 55 | 3 | 1 | 105 | 146 | 254 | 1.54e-21 | 85.9 |
| MsG0380014874.01.T01 | MTR_7g104720 | 48.598 | 107 | 50 | 4 | 1 | 105 | 301 | 404 | 9.68e-21 | 85.5 |
| MsG0380014874.01.T01 | MTR_7g106500 | 41.739 | 115 | 55 | 3 | 1 | 105 | 166 | 278 | 2.25e-18 | 77.8 |
| MsG0380014874.01.T01 | MTR_6g011640 | 37.097 | 124 | 59 | 3 | 1 | 105 | 273 | 396 | 2.10e-15 | 70.5 |
| MsG0380014874.01.T01 | MTR_7g010090 | 42.400 | 125 | 52 | 3 | 1 | 105 | 307 | 431 | 3.64e-15 | 70.1 |
| MsG0380014874.01.T01 | MTR_8g106900 | 43.363 | 113 | 56 | 4 | 1 | 106 | 163 | 274 | 2.21e-14 | 67.0 |
| MsG0380014874.01.T01 | MTR_8g099665 | 41.304 | 92 | 50 | 2 | 18 | 105 | 276 | 367 | 1.36e-13 | 65.5 |
| MsG0380014874.01.T01 | MTR_3g030520 | 35.484 | 124 | 61 | 3 | 1 | 105 | 175 | 298 | 3.51e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014874.01.T01 | AT5G58530 | 54.386 | 114 | 44 | 1 | 1 | 106 | 153 | 266 | 6.96e-36 | 123 |
| MsG0380014874.01.T01 | AT2G41330 | 45.794 | 107 | 56 | 2 | 1 | 105 | 294 | 400 | 5.06e-26 | 100 |
| MsG0380014874.01.T01 | AT5G13810 | 47.222 | 108 | 54 | 3 | 1 | 106 | 167 | 273 | 1.14e-25 | 97.1 |
| MsG0380014874.01.T01 | AT3G11773 | 43.396 | 106 | 58 | 1 | 1 | 106 | 47 | 150 | 5.70e-25 | 92.0 |
| MsG0380014874.01.T01 | AT3G11773 | 43.396 | 106 | 58 | 1 | 1 | 106 | 97 | 200 | 9.93e-25 | 92.8 |
| MsG0380014874.01.T01 | AT5G06470 | 44.348 | 115 | 51 | 4 | 1 | 106 | 129 | 239 | 2.91e-23 | 90.1 |
| MsG0380014874.01.T01 | AT3G57070 | 43.519 | 108 | 58 | 3 | 1 | 105 | 308 | 415 | 1.08e-22 | 90.9 |
| MsG0380014874.01.T01 | AT5G01420 | 40.566 | 106 | 63 | 0 | 1 | 106 | 296 | 401 | 2.69e-21 | 87.0 |
| MsG0380014874.01.T01 | AT5G39865 | 41.129 | 124 | 54 | 3 | 1 | 105 | 265 | 388 | 1.57e-20 | 84.7 |
| MsG0380014874.01.T01 | AT1G64500 | 42.982 | 114 | 56 | 4 | 1 | 105 | 252 | 365 | 2.95e-20 | 84.0 |
| MsG0380014874.01.T01 | AT3G28850 | 40.441 | 136 | 50 | 3 | 1 | 105 | 291 | 426 | 1.60e-19 | 82.4 |
| MsG0380014874.01.T01 | AT5G03870 | 43.119 | 109 | 58 | 3 | 1 | 106 | 277 | 384 | 1.81e-14 | 67.8 |
| MsG0380014874.01.T01 | AT1G32760 | 41.228 | 114 | 59 | 4 | 1 | 106 | 201 | 314 | 4.35e-13 | 63.9 |
| MsG0380014874.01.T01 | AT4G10630 | 36.134 | 119 | 63 | 3 | 1 | 106 | 216 | 334 | 2.03e-12 | 62.0 |
Find 36 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTGTAGCCACGTCACTTCT+TGG | 0.210642 | 3:+61817168 | None:intergenic |
| ATCATAGGTTTGTGCTTTGT+GGG | 0.360656 | 3:-61817073 | MsG0380014874.01.T01:CDS |
| GTAATGAGAGTGGTTTAATC+AGG | 0.401983 | 3:-61816989 | MsG0380014874.01.T01:CDS |
| GTGCTTTGTGGGGAGTGTTC+CGG | 0.407345 | 3:-61817062 | MsG0380014874.01.T01:CDS |
| TCGGAGCTTCGTTTAGTAAC+GGG | 0.432552 | 3:-61817251 | MsG0380014874.01.T01:CDS |
| TTAGTAACGGGTCATAAAAC+CGG | 0.433969 | 3:-61817239 | MsG0380014874.01.T01:CDS |
| GTGTAGCCACGTCACTTCTT+GGG | 0.449243 | 3:+61817169 | None:intergenic |
| GATCATAGGTTTGTGCTTTG+TGG | 0.450981 | 3:-61817074 | MsG0380014874.01.T01:CDS |
| CTCGGAGCTTCGTTTAGTAA+CGG | 0.461019 | 3:-61817252 | MsG0380014874.01.T01:CDS |
| GGAGTGTTCCGGTGCGCGTA+AGG | 0.488430 | 3:-61817051 | MsG0380014874.01.T01:CDS |
| GGTGGGTTTAAGACTTGTAC+GGG | 0.492107 | 3:-61817014 | MsG0380014874.01.T01:CDS |
| GCCACGTGTGCGGTGATCAT+AGG | 0.494610 | 3:-61817088 | MsG0380014874.01.T01:CDS |
| CGCATACACCTTACGCGCAC+CGG | 0.497139 | 3:+61817043 | None:intergenic |
| AGGTGGGTTTAAGACTTGTA+CGG | 0.507952 | 3:-61817015 | MsG0380014874.01.T01:CDS |
| CGTAAGGTGTATGCGGAGAA+AGG | 0.523916 | 3:-61817035 | MsG0380014874.01.T01:CDS |
| CACACTCGCGAATCCGCTAC+GGG | 0.543080 | 3:+61817110 | None:intergenic |
| GCACACTCGCGAATCCGCTA+CGG | 0.554411 | 3:+61817109 | None:intergenic |
| TTCATCAACGGAAGATACAT+AGG | 0.568426 | 3:-61817197 | MsG0380014874.01.T01:CDS |
| GTGACGTGGCTACACGAGAA+TGG | 0.570011 | 3:-61817161 | MsG0380014874.01.T01:CDS |
| AACACGTGGCAGTTTCAACC+CGG | 0.573973 | 3:+61817220 | None:intergenic |
| AGGTGTATGCGGAGAAAGGT+GGG | 0.585150 | 3:-61817031 | MsG0380014874.01.T01:CDS |
| TGTACGGGTTGTAATGAGAG+TGG | 0.600336 | 3:-61816999 | MsG0380014874.01.T01:CDS |
| TAGTAACGGGTCATAAAACC+GGG | 0.604961 | 3:-61817238 | MsG0380014874.01.T01:CDS |
| AAGGTGTATGCGGAGAAAGG+TGG | 0.611895 | 3:-61817032 | MsG0380014874.01.T01:CDS |
| CGTGACGTGTCAATGGACTC+TGG | 0.615576 | 3:-61817281 | None:intergenic |
| TCAACGGAAGATACATAGGT+GGG | 0.617802 | 3:-61817193 | MsG0380014874.01.T01:CDS |
| TCATAGGTTTGTGCTTTGTG+GGG | 0.618862 | 3:-61817072 | MsG0380014874.01.T01:CDS |
| GAGCTGAAGAAGCTTCTCGA+AGG | 0.624095 | 3:-61817137 | MsG0380014874.01.T01:CDS |
| ACCTATGATCACCGCACACG+TGG | 0.625557 | 3:+61817087 | None:intergenic |
| ATCAACGGAAGATACATAGG+TGG | 0.658644 | 3:-61817194 | MsG0380014874.01.T01:CDS |
| TCGCGAGTGTGCCACGTGTG+CGG | 0.672708 | 3:-61817098 | MsG0380014874.01.T01:CDS |
| CGGTGCGCGTAAGGTGTATG+CGG | 0.674471 | 3:-61817042 | MsG0380014874.01.T01:CDS |
| CAACGGAAGATACATAGGTG+GGG | 0.674486 | 3:-61817192 | MsG0380014874.01.T01:CDS |
| TCTCGAAGGATTGCCCGTAG+CGG | 0.688628 | 3:-61817123 | MsG0380014874.01.T01:CDS |
| CTTCCGTTGATGAAAACACG+TGG | 0.689155 | 3:+61817206 | None:intergenic |
| GTGGGGCCCAAGAAGTGACG+TGG | 0.703401 | 3:-61817175 | MsG0380014874.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATCATAGGTTTGTGCTTTGT+GGG | - | Chr3:61817164-61817183 | MsG0380014874.01.T01:CDS | 35.0% | |
| GTAATGAGAGTGGTTTAATC+AGG | - | Chr3:61817248-61817267 | MsG0380014874.01.T01:CDS | 35.0% | |
| TTCATCAACGGAAGATACAT+AGG | - | Chr3:61817040-61817059 | MsG0380014874.01.T01:CDS | 35.0% | |
| !! | TTAGTAACGGGTCATAAAAC+CGG | - | Chr3:61816998-61817017 | MsG0380014874.01.T01:CDS | 35.0% |
| ATCAACGGAAGATACATAGG+TGG | - | Chr3:61817043-61817062 | MsG0380014874.01.T01:CDS | 40.0% | |
| GATCATAGGTTTGTGCTTTG+TGG | - | Chr3:61817163-61817182 | MsG0380014874.01.T01:CDS | 40.0% | |
| TCAACGGAAGATACATAGGT+GGG | - | Chr3:61817044-61817063 | MsG0380014874.01.T01:CDS | 40.0% | |
| TCATAGGTTTGTGCTTTGTG+GGG | - | Chr3:61817165-61817184 | MsG0380014874.01.T01:CDS | 40.0% | |
| ! | AGGTGGGTTTAAGACTTGTA+CGG | - | Chr3:61817222-61817241 | MsG0380014874.01.T01:CDS | 40.0% |
| !! | TAGTAACGGGTCATAAAACC+GGG | - | Chr3:61816999-61817018 | MsG0380014874.01.T01:CDS | 40.0% |
| CAACGGAAGATACATAGGTG+GGG | - | Chr3:61817045-61817064 | MsG0380014874.01.T01:CDS | 45.0% | |
| CTCGGAGCTTCGTTTAGTAA+CGG | - | Chr3:61816985-61817004 | MsG0380014874.01.T01:CDS | 45.0% | |
| TCGGAGCTTCGTTTAGTAAC+GGG | - | Chr3:61816986-61817005 | MsG0380014874.01.T01:CDS | 45.0% | |
| ! | CTGCCACGTGTTTTCATCAA+CGG | - | Chr3:61817028-61817047 | MsG0380014874.01.T01:CDS | 45.0% |
| ! | CTTCCGTTGATGAAAACACG+TGG | + | Chr3:61817034-61817053 | None:intergenic | 45.0% |
| ! | GGTGGGTTTAAGACTTGTAC+GGG | - | Chr3:61817223-61817242 | MsG0380014874.01.T01:CDS | 45.0% |
| ! | TGTACGGGTTGTAATGAGAG+TGG | - | Chr3:61817238-61817257 | MsG0380014874.01.T01:CDS | 45.0% |
| AACACGTGGCAGTTTCAACC+CGG | + | Chr3:61817020-61817039 | None:intergenic | 50.0% | |
| AAGGTGTATGCGGAGAAAGG+TGG | - | Chr3:61817205-61817224 | MsG0380014874.01.T01:CDS | 50.0% | |
| AGGTGTATGCGGAGAAAGGT+GGG | - | Chr3:61817206-61817225 | MsG0380014874.01.T01:CDS | 50.0% | |
| CGTAAGGTGTATGCGGAGAA+AGG | - | Chr3:61817202-61817221 | MsG0380014874.01.T01:CDS | 50.0% | |
| GAGCTGAAGAAGCTTCTCGA+AGG | - | Chr3:61817100-61817119 | MsG0380014874.01.T01:CDS | 50.0% | |
| GTGTAGCCACGTCACTTCTT+GGG | + | Chr3:61817071-61817090 | None:intergenic | 50.0% | |
| ACCTATGATCACCGCACACG+TGG | + | Chr3:61817153-61817172 | None:intergenic | 55.0% | |
| CGTGTAGCCACGTCACTTCT+TGG | + | Chr3:61817072-61817091 | None:intergenic | 55.0% | |
| GTGACGTGGCTACACGAGAA+TGG | - | Chr3:61817076-61817095 | MsG0380014874.01.T01:CDS | 55.0% | |
| TCTCGAAGGATTGCCCGTAG+CGG | - | Chr3:61817114-61817133 | MsG0380014874.01.T01:CDS | 55.0% | |
| !! | GTGCTTTGTGGGGAGTGTTC+CGG | - | Chr3:61817175-61817194 | MsG0380014874.01.T01:CDS | 55.0% |
| CACACTCGCGAATCCGCTAC+GGG | + | Chr3:61817130-61817149 | None:intergenic | 60.0% | |
| CGCATACACCTTACGCGCAC+CGG | + | Chr3:61817197-61817216 | None:intergenic | 60.0% | |
| CGGTGCGCGTAAGGTGTATG+CGG | - | Chr3:61817195-61817214 | MsG0380014874.01.T01:CDS | 60.0% | |
| GCACACTCGCGAATCCGCTA+CGG | + | Chr3:61817131-61817150 | None:intergenic | 60.0% | |
| GCCACGTGTGCGGTGATCAT+AGG | - | Chr3:61817149-61817168 | MsG0380014874.01.T01:CDS | 60.0% | |
| GTGGGGCCCAAGAAGTGACG+TGG | - | Chr3:61817062-61817081 | MsG0380014874.01.T01:CDS | 65.0% | |
| TCGCGAGTGTGCCACGTGTG+CGG | - | Chr3:61817139-61817158 | MsG0380014874.01.T01:CDS | 65.0% | |
| ! | GGAGTGTTCCGGTGCGCGTA+AGG | - | Chr3:61817186-61817205 | MsG0380014874.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 61816968 | 61817291 | 61816968 | ID=MsG0380014874.01;Name=MsG0380014874.01 |
| Chr3 | mRNA | 61816968 | 61817291 | 61816968 | ID=MsG0380014874.01.T01;Parent=MsG0380014874.01;Name=MsG0380014874.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|1|1|0|107 |
| Chr3 | exon | 61816968 | 61817291 | 61816968 | ID=MsG0380014874.01.T01:exon:29344;Parent=MsG0380014874.01.T01 |
| Chr3 | CDS | 61816968 | 61817291 | 61816968 | ID=MsG0380014874.01.T01:cds;Parent=MsG0380014874.01.T01 |
| Gene Sequence |
| Protein sequence |
