Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036136.01.T01 | MCI03036.1 | 86.885 | 122 | 14 | 1 | 1 | 120 | 64 | 185 | 1.08E-67 | 212 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036136.01.T01 | A0A396GSL7 | 90.984 | 122 | 9 | 1 | 1 | 120 | 836 | 957 | 1.06e-64 | 221 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048856.01 | MsG0780036136.01 | 0.811882 | 5.605169e-51 | 3.510525e-48 |
| MsG0180000139.01 | MsG0780036136.01 | 0.817316 | 3.512569e-52 | 2.549456e-49 |
| MsG0180000145.01 | MsG0780036136.01 | 0.809640 | 1.712010e-50 | 1.010660e-47 |
| MsG0180000334.01 | MsG0780036136.01 | 0.809689 | 1.671378e-50 | 9.879118e-48 |
| MsG0180000335.01 | MsG0780036136.01 | 0.800818 | 1.205752e-48 | 5.668518e-46 |
| MsG0180000835.01 | MsG0780036136.01 | 0.821514 | 3.876743e-53 | 3.160542e-50 |
| MsG0180001892.01 | MsG0780036136.01 | 0.810184 | 1.307549e-50 | 7.829011e-48 |
| MsG0180003493.01 | MsG0780036136.01 | 0.839848 | 1.239378e-57 | 1.733319e-54 |
| MsG0180003786.01 | MsG0780036136.01 | 0.805837 | 1.100756e-49 | 5.883766e-47 |
| MsG0180003920.01 | MsG0780036136.01 | 0.816237 | 6.133259e-52 | 4.322161e-49 |
| MsG0180004389.01 | MsG0780036136.01 | 0.819380 | 1.197124e-52 | 9.196739e-50 |
| MsG0180004481.01 | MsG0780036136.01 | 0.809082 | 2.255535e-50 | 1.311767e-47 |
| MsG0180004482.01 | MsG0780036136.01 | 0.813417 | 2.587557e-51 | 1.688832e-48 |
| MsG0180004754.01 | MsG0780036136.01 | 0.808404 | 3.149487e-50 | 1.799229e-47 |
| MsG0180004928.01 | MsG0780036136.01 | 0.807294 | 5.423465e-50 | 3.009525e-47 |
| MsG0180005049.01 | MsG0780036136.01 | 0.809148 | 2.183870e-50 | 1.272349e-47 |
| MsG0180005575.01 | MsG0780036136.01 | 0.836373 | 9.720254e-57 | 1.222610e-53 |
| MsG0180006224.01 | MsG0780036136.01 | 0.833700 | 4.586393e-56 | 5.323052e-53 |
| MsG0280006405.01 | MsG0780036136.01 | 0.803525 | 3.344281e-49 | 1.684435e-46 |
| MsG0280011014.01 | MsG0780036136.01 | 0.817477 | 3.231867e-52 | 2.356238e-49 |
| MsG0280011339.01 | MsG0780036136.01 | 0.825482 | 4.576926e-54 | 4.176795e-51 |
| MsG0280011388.01 | MsG0780036136.01 | 0.838184 | 3.343483e-57 | 4.443342e-54 |
| MsG0380011609.01 | MsG0780036136.01 | 0.810594 | 1.066555e-50 | 6.455891e-48 |
| MsG0380012471.01 | MsG0780036136.01 | 0.821439 | 4.035023e-53 | 3.282759e-50 |
| MsG0380013183.01 | MsG0780036136.01 | 0.815127 | 1.083684e-51 | 7.407859e-49 |
| MsG0380013475.01 | MsG0780036136.01 | 0.839595 | 1.442749e-57 | 2.001977e-54 |
| MsG0380013549.01 | MsG0780036136.01 | 0.805448 | 1.328435e-49 | 7.029954e-47 |
| MsG0380014537.01 | MsG0780036136.01 | 0.808250 | 3.396238e-50 | 1.932426e-47 |
| MsG0380014701.01 | MsG0780036136.01 | 0.801514 | 8.686507e-49 | 4.156298e-46 |
| MsG0380014874.01 | MsG0780036136.01 | 0.807494 | 4.918206e-50 | 2.743428e-47 |
| MsG0680030881.01 | MsG0780036136.01 | 0.804054 | 2.596887e-49 | 1.325884e-46 |
| MsG0680031488.01 | MsG0780036136.01 | 0.800097 | 1.690756e-48 | 7.804708e-46 |
| MsG0680031549.01 | MsG0780036136.01 | 0.812487 | 4.137003e-51 | 2.633875e-48 |
| MsG0480018894.01 | MsG0780036136.01 | 0.835297 | 1.821677e-56 | 2.218539e-53 |
| MsG0480019350.01 | MsG0780036136.01 | 0.802861 | 4.588153e-49 | 2.271893e-46 |
| MsG0480020739.01 | MsG0780036136.01 | 0.803539 | 3.322432e-49 | 1.674018e-46 |
| MsG0480020780.01 | MsG0780036136.01 | 0.817086 | 3.958149e-52 | 2.854813e-49 |
| MsG0480021037.01 | MsG0780036136.01 | 0.802142 | 6.454678e-49 | 3.138043e-46 |
| MsG0480021195.01 | MsG0780036136.01 | 0.805501 | 1.294824e-49 | 6.861295e-47 |
| MsG0480022855.01 | MsG0780036136.01 | 0.805419 | 1.346925e-49 | 7.122496e-47 |
| MsG0480023172.01 | MsG0780036136.01 | 0.810067 | 1.385501e-50 | 8.270907e-48 |
| MsG0480023221.01 | MsG0780036136.01 | 0.807485 | 4.938939e-50 | 2.754329e-47 |
| MsG0480023272.01 | MsG0780036136.01 | 0.807723 | 4.398003e-50 | 2.467882e-47 |
| MsG0480023463.01 | MsG0780036136.01 | 0.838975 | 2.089539e-57 | 2.844907e-54 |
| MsG0480023657.01 | MsG0780036136.01 | 0.803630 | 3.181263e-49 | 1.606531e-46 |
| MsG0580024185.01 | MsG0780036136.01 | 0.802069 | 6.681962e-49 | 3.242568e-46 |
| MsG0580024197.01 | MsG0780036136.01 | 0.815140 | 1.076682e-51 | 7.362340e-49 |
| MsG0580024463.01 | MsG0780036136.01 | 0.804607 | 1.991316e-49 | 1.031214e-46 |
| MsG0580025587.01 | MsG0780036136.01 | 0.802748 | 4.841094e-49 | 2.390292e-46 |
| MsG0580026059.01 | MsG0780036136.01 | 0.808300 | 3.313671e-50 | 1.887849e-47 |
| MsG0380015332.01 | MsG0780036136.01 | 0.812866 | 3.417459e-51 | 2.197354e-48 |
| MsG0380015555.01 | MsG0780036136.01 | 0.824430 | 8.106420e-54 | 7.178272e-51 |
| MsG0380015677.01 | MsG0780036136.01 | 0.813384 | 2.630808e-51 | 1.715559e-48 |
| MsG0380015831.01 | MsG0780036136.01 | 0.813805 | 2.125686e-51 | 1.402047e-48 |
| MsG0380015944.01 | MsG0780036136.01 | 0.818418 | 1.980422e-52 | 1.481736e-49 |
| MsG0380016131.01 | MsG0780036136.01 | 0.805278 | 1.442255e-49 | 7.598651e-47 |
| MsG0380016247.01 | MsG0780036136.01 | 0.832785 | 7.753354e-56 | 8.756141e-53 |
| MsG0380016407.01 | MsG0780036136.01 | 0.804173 | 2.453332e-49 | 1.256346e-46 |
| MsG0380016505.01 | MsG0780036136.01 | 0.803223 | 3.863094e-49 | 1.930580e-46 |
| MsG0380016970.01 | MsG0780036136.01 | 0.836349 | 9.859219e-57 | 1.239290e-53 |
| MsG0480018313.01 | MsG0780036136.01 | 0.812487 | 4.135923e-51 | 2.633253e-48 |
| MsG0580026593.01 | MsG0780036136.01 | 0.801866 | 7.355264e-49 | 3.551027e-46 |
| MsG0580027091.01 | MsG0780036136.01 | 0.817846 | 2.667980e-52 | 1.964854e-49 |
| MsG0580027431.01 | MsG0780036136.01 | 0.842794 | 2.079943e-58 | 3.188683e-55 |
| MsG0580027925.01 | MsG0780036136.01 | 0.812188 | 4.808110e-51 | 3.036553e-48 |
| MsG0580028060.01 | MsG0780036136.01 | 0.818587 | 1.813587e-52 | 1.363083e-49 |
| MsG0580028517.01 | MsG0780036136.01 | 0.803674 | 3.114781e-49 | 1.574705e-46 |
| MsG0580028897.01 | MsG0780036136.01 | 0.804744 | 1.864681e-49 | 9.691488e-47 |
| MsG0580029127.01 | MsG0780036136.01 | 0.805032 | 1.623710e-49 | 8.500931e-47 |
| MsG0580029236.01 | MsG0780036136.01 | 0.800821 | 1.203903e-48 | 5.660383e-46 |
| MsG0580029729.01 | MsG0780036136.01 | 0.834909 | 2.281821e-56 | 2.746393e-53 |
| MsG0280006930.01 | MsG0780036136.01 | 0.802201 | 6.276115e-49 | 3.055776e-46 |
| MsG0280007226.01 | MsG0780036136.01 | 0.801160 | 1.026458e-48 | 4.867870e-46 |
| MsG0280007452.01 | MsG0780036136.01 | 0.808750 | 2.656463e-50 | 1.531290e-47 |
| MsG0280007527.01 | MsG0780036136.01 | 0.811852 | 5.689596e-51 | 3.560603e-48 |
| MsG0280007955.01 | MsG0780036136.01 | 0.820757 | 5.794131e-53 | 4.625368e-50 |
| MsG0280008378.01 | MsG0780036136.01 | 0.813735 | 2.201736e-51 | 1.449487e-48 |
| MsG0280008457.01 | MsG0780036136.01 | 0.814162 | 1.772495e-51 | 1.180458e-48 |
| MsG0280009794.01 | MsG0780036136.01 | 0.844873 | 5.773451e-59 | 9.449083e-56 |
| MsG0280009983.01 | MsG0780036136.01 | 0.803925 | 2.762635e-49 | 1.405863e-46 |
| MsG0280010015.01 | MsG0780036136.01 | 0.823058 | 1.699352e-53 | 1.447666e-50 |
| MsG0280010176.01 | MsG0780036136.01 | 0.815006 | 1.152906e-51 | 7.855723e-49 |
| MsG0280010491.01 | MsG0780036136.01 | 0.800127 | 1.667508e-48 | 7.703369e-46 |
| MsG0680035876.01 | MsG0780036136.01 | 0.801761 | 7.730220e-49 | 3.721780e-46 |
| MsG0680035888.01 | MsG0780036136.01 | 0.818421 | 1.978073e-52 | 1.480071e-49 |
| MsG0780035979.01 | MsG0780036136.01 | 0.801708 | 7.927379e-49 | 3.811622e-46 |
| MsG0780036136.01 | MsG0780036477.01 | 0.812600 | 3.908126e-51 | 2.495432e-48 |
| MsG0780036136.01 | MsG0780036815.01 | 0.834113 | 3.615109e-56 | 4.248536e-53 |
| MsG0780036136.01 | MsG0780037054.01 | 0.802716 | 4.916058e-49 | 2.425322e-46 |
| MsG0780036136.01 | MsG0780037093.01 | 0.815458 | 9.149007e-52 | 6.311593e-49 |
| MsG0780036136.01 | MsG0780038603.01 | 0.824572 | 7.504447e-54 | 6.673367e-51 |
| MsG0780036136.01 | MsG0780039137.01 | 0.802027 | 6.815298e-49 | 3.303692e-46 |
| MsG0780036136.01 | MsG0780039170.01 | 0.819581 | 1.077366e-52 | 8.324022e-50 |
| MsG0780036136.01 | MsG0780039243.01 | 0.810842 | 9.428168e-51 | 5.744921e-48 |
| MsG0780036136.01 | MsG0780039443.01 | 0.800051 | 1.727575e-48 | 7.965408e-46 |
| MsG0780036136.01 | MsG0780039520.01 | 0.807309 | 5.382634e-50 | 2.988105e-47 |
| MsG0780036136.01 | MsG0780040044.01 | 0.801132 | 1.039946e-48 | 4.928512e-46 |
| MsG0780036136.01 | MsG0780040926.01 | 0.830017 | 3.720176e-55 | 3.872047e-52 |
| MsG0780036136.01 | MsG0780041016.01 | 0.809672 | 1.685446e-50 | 9.957890e-48 |
| MsG0780036136.01 | MsG0880041986.01 | 0.820133 | 8.057807e-53 | 6.322694e-50 |
| MsG0780036136.01 | MsG0880042128.01 | 0.808145 | 3.575477e-50 | 2.028753e-47 |
| MsG0780036136.01 | MsG0880042129.01 | 0.804513 | 2.083371e-49 | 1.076222e-46 |
| MsG0780036136.01 | MsG0880043937.01 | 0.814282 | 1.668286e-51 | 1.114683e-48 |
| MsG0780036136.01 | MsG0880044555.01 | 0.815451 | 9.184844e-52 | 6.334843e-49 |
| MsG0780036136.01 | MsG0880044782.01 | 0.833481 | 5.203728e-56 | 6.000236e-53 |
| MsG0780036136.01 | MsG0880045294.01 | 0.820865 | 5.472130e-53 | 4.381785e-50 |
| MsG0780036136.01 | MsG0880045453.01 | 0.807094 | 5.978378e-50 | 3.300122e-47 |
| MsG0780036136.01 | MsG0880046012.01 | 0.814231 | 1.711385e-51 | 1.141908e-48 |
| MsG0780036136.01 | MsG0880046284.01 | 0.823012 | 1.741100e-53 | 1.481337e-50 |
| MsG0780036136.01 | MsG0880047472.01 | 0.800520 | 1.386750e-48 | 6.470546e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036136.01.T01 | MTR_7g007880 | 90.984 | 122 | 9 | 1 | 1 | 120 | 798 | 919 | 2.32e-68 | 221 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATTAGGCTTCAGAAAAT+TGG | 0.197443 | 7:-3272338 | MsG0780036136.01.T01:CDS |
| TCCACACCCTTTGATGGTTC+TGG | 0.237883 | 7:+3272494 | None:intergenic |
| TCTGAATATGTCAATGATTC+AGG | 0.305872 | 7:-3272416 | MsG0780036136.01.T01:CDS |
| AAGTCTTCCACACCCTTTGA+TGG | 0.343754 | 7:+3272488 | None:intergenic |
| AACGAAACCTCATCATGATT+TGG | 0.365971 | 7:+3272542 | None:intergenic |
| TCTTTGGTGACAACAATGTT+AGG | 0.406561 | 7:-3272286 | MsG0780036136.01.T01:CDS |
| ATCCCTTTGAATAGAAGATC+TGG | 0.441789 | 7:+3272593 | None:intergenic |
| ATCAAAGGGTGTGGAAGACT+TGG | 0.473084 | 7:-3272486 | MsG0780036136.01.T01:CDS |
| GAACCAGATCTTCTATTCAA+AGG | 0.481988 | 7:-3272596 | MsG0780036136.01.T01:CDS |
| AACCAGATCTTCTATTCAAA+GGG | 0.482771 | 7:-3272595 | MsG0780036136.01.T01:CDS |
| GGATCAATTGAAAGATCAAA+AGG | 0.485031 | 7:-3272317 | MsG0780036136.01.T01:CDS |
| AAATCATGATGAGGTTTCGT+TGG | 0.512566 | 7:-3272540 | MsG0780036136.01.T01:CDS |
| CCAGGTTCAAAGCTCAGATT+AGG | 0.514858 | 7:-3272352 | MsG0780036136.01.T01:CDS |
| TAACAAGCTTGAGAGCCTCC+AGG | 0.518168 | 7:-3272462 | MsG0780036136.01.T01:CDS |
| ATATGTCAATGATTCAGGAT+TGG | 0.521804 | 7:-3272411 | MsG0780036136.01.T01:CDS |
| CCTAATCTGAGCTTTGAACC+TGG | 0.530704 | 7:+3272352 | None:intergenic |
| CATCTTCGTCCGAGCTACCC+TGG | 0.534803 | 7:+3272444 | None:intergenic |
| AGATAAACCAGAACCATCAA+AGG | 0.535238 | 7:-3272501 | MsG0780036136.01.T01:CDS |
| TGAGAGCCTCCAGGGTAGCT+CGG | 0.536419 | 7:-3272453 | MsG0780036136.01.T01:CDS |
| AAGCTAGTGAATTTATCGCC+AGG | 0.536727 | 7:-3272370 | MsG0780036136.01.T01:CDS |
| ACCAGAACCATCAAAGGGTG+TGG | 0.544869 | 7:-3272495 | MsG0780036136.01.T01:CDS |
| GATAAACCAGAACCATCAAA+GGG | 0.583954 | 7:-3272500 | MsG0780036136.01.T01:CDS |
| AACAAGCTTGAGAGCCTCCA+GGG | 0.587449 | 7:-3272461 | MsG0780036136.01.T01:CDS |
| GGGTAGCTCGGACGAAGATG+TGG | 0.598451 | 7:-3272441 | MsG0780036136.01.T01:CDS |
| TGATTCAGGATTGGATTCTG+AGG | 0.599780 | 7:-3272402 | MsG0780036136.01.T01:CDS |
| TTCAGGATTGGATTCTGAGG+TGG | 0.603058 | 7:-3272399 | MsG0780036136.01.T01:CDS |
| CTTCGTCCGAGCTACCCTGG+AGG | 0.707835 | 7:+3272447 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCAAAAGGTCAAAAAATCTT+TGG | - | Chr7:3272604-3272623 | MsG0780036136.01.T01:CDS | 25.0% |
| AACCAGATCTTCTATTCAAA+GGG | - | Chr7:3272311-3272330 | MsG0780036136.01.T01:CDS | 30.0% | |
| ATATGTCAATGATTCAGGAT+TGG | - | Chr7:3272495-3272514 | MsG0780036136.01.T01:CDS | 30.0% | |
| GGATCAATTGAAAGATCAAA+AGG | - | Chr7:3272589-3272608 | MsG0780036136.01.T01:CDS | 30.0% | |
| TCTGAATATGTCAATGATTC+AGG | - | Chr7:3272490-3272509 | MsG0780036136.01.T01:CDS | 30.0% | |
| ! | CTATTTTCCAAATCATGATG+AGG | - | Chr7:3272357-3272376 | MsG0780036136.01.T01:CDS | 30.0% |
| AAATCATGATGAGGTTTCGT+TGG | - | Chr7:3272366-3272385 | MsG0780036136.01.T01:CDS | 35.0% | |
| AACGAAACCTCATCATGATT+TGG | + | Chr7:3272367-3272386 | None:intergenic | 35.0% | |
| AGATAAACCAGAACCATCAA+AGG | - | Chr7:3272405-3272424 | MsG0780036136.01.T01:CDS | 35.0% | |
| ATCCCTTTGAATAGAAGATC+TGG | + | Chr7:3272316-3272335 | None:intergenic | 35.0% | |
| CAGATTAGGCTTCAGAAAAT+TGG | - | Chr7:3272568-3272587 | MsG0780036136.01.T01:CDS | 35.0% | |
| GAACCAGATCTTCTATTCAA+AGG | - | Chr7:3272310-3272329 | MsG0780036136.01.T01:CDS | 35.0% | |
| GATAAACCAGAACCATCAAA+GGG | - | Chr7:3272406-3272425 | MsG0780036136.01.T01:CDS | 35.0% | |
| ! | TCTTTGGTGACAACAATGTT+AGG | - | Chr7:3272620-3272639 | MsG0780036136.01.T01:CDS | 35.0% |
| AAGCTAGTGAATTTATCGCC+AGG | - | Chr7:3272536-3272555 | MsG0780036136.01.T01:CDS | 40.0% | |
| TGATTCAGGATTGGATTCTG+AGG | - | Chr7:3272504-3272523 | MsG0780036136.01.T01:CDS | 40.0% | |
| AAGTCTTCCACACCCTTTGA+TGG | + | Chr7:3272421-3272440 | None:intergenic | 45.0% | |
| CCAGGTTCAAAGCTCAGATT+AGG | - | Chr7:3272554-3272573 | MsG0780036136.01.T01:CDS | 45.0% | |
| ! | ATCAAAGGGTGTGGAAGACT+TGG | - | Chr7:3272420-3272439 | MsG0780036136.01.T01:CDS | 45.0% |
| ! | TTCAGGATTGGATTCTGAGG+TGG | - | Chr7:3272507-3272526 | MsG0780036136.01.T01:CDS | 45.0% |
| !! | CCTAATCTGAGCTTTGAACC+TGG | + | Chr7:3272557-3272576 | None:intergenic | 45.0% |
| AACAAGCTTGAGAGCCTCCA+GGG | - | Chr7:3272445-3272464 | MsG0780036136.01.T01:CDS | 50.0% | |
| ACCAGAACCATCAAAGGGTG+TGG | - | Chr7:3272411-3272430 | MsG0780036136.01.T01:CDS | 50.0% | |
| TAACAAGCTTGAGAGCCTCC+AGG | - | Chr7:3272444-3272463 | MsG0780036136.01.T01:CDS | 50.0% | |
| ! | TCCACACCCTTTGATGGTTC+TGG | + | Chr7:3272415-3272434 | None:intergenic | 50.0% |
| CATCTTCGTCCGAGCTACCC+TGG | + | Chr7:3272465-3272484 | None:intergenic | 60.0% | |
| GGGTAGCTCGGACGAAGATG+TGG | - | Chr7:3272465-3272484 | MsG0780036136.01.T01:CDS | 60.0% | |
| TGAGAGCCTCCAGGGTAGCT+CGG | - | Chr7:3272453-3272472 | MsG0780036136.01.T01:CDS | 60.0% | |
| CTTCGTCCGAGCTACCCTGG+AGG | + | Chr7:3272462-3272481 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 3272283 | 3272645 | 3272283 | ID=MsG0780036136.01;Name=MsG0780036136.01 |
| Chr7 | mRNA | 3272283 | 3272645 | 3272283 | ID=MsG0780036136.01.T01;Parent=MsG0780036136.01;Name=MsG0780036136.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|120 |
| Chr7 | exon | 3272283 | 3272645 | 3272283 | ID=MsG0780036136.01.T01:exon:15908;Parent=MsG0780036136.01.T01 |
| Chr7 | CDS | 3272283 | 3272645 | 3272283 | ID=MsG0780036136.01.T01:cds;Parent=MsG0780036136.01.T01 |
| Gene Sequence |
| Protein sequence |
