AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048532.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048532.01.T01 MTR_8g099150 97.468 79 2 0 95 173 28 106 1.69e-51 160
MsG0080048532.01.T01 MTR_8g016300 96.203 79 3 0 95 173 28 106 8.15e-51 159
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048532.01.T01 AT5G55140 59.783 92 35 2 84 173 14 105 3.98e-30 106

Find 65 sgRNAs with CRISPR-Local

Find 229 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGCAATGTTTGCTGATA+TGG 0.161816 contig349end:+6641 MsG0080048532.01.T01:three_prime_UTR
ACTTAGGCATTTGAAGATTT+TGG 0.175275 contig349end:-6675 None:intergenic
AGTGCGCCTATGTTGCTTTC+TGG 0.240202 contig349end:-6323 None:intergenic
AACGCTAGTGAGGGGTATTC+CGG 0.288083 contig349end:+6298 MsG0080048532.01.T01:CDS
TGGATCCAATGTTAAGGTTT+AGG 0.303179 contig349end:+3791 MsG0080048532.01.T01:CDS
GGTTTCCTCCTTCTCTTTGC+GGG 0.349900 contig349end:-6557 None:intergenic
GGGGAAGGGAATGGAGGGTT+TGG 0.352226 contig349end:+5719 MsG0080048532.01.T01:CDS
ACAGTTTATCAATTTCAGTT+TGG 0.359298 contig349end:+6820 MsG0080048532.01.T01:three_prime_UTR
TCTTCAAATGCCTAAGTTTG+AGG 0.363190 contig349end:+6681 MsG0080048532.01.T01:three_prime_UTR
GTTTGAGGTTGTGCCTCATA+TGG 0.383938 contig349end:+6696 MsG0080048532.01.T01:three_prime_UTR
TCAACCTGCTTCTTCTGCTT+AGG 0.384301 contig349end:+6615 MsG0080048532.01.T01:CDS
TAAGGCTTTCTTTGCGAGTT+CGG 0.386685 contig349end:+5695 MsG0080048532.01.T01:CDS
ATGTTAAGGTTTAGGTTGTT+TGG 0.388298 contig349end:+3799 MsG0080048532.01.T01:CDS
CAACCTGCTTCTTCTGCTTA+GGG 0.390606 contig349end:+6616 MsG0080048532.01.T01:CDS
GATGGAACACACCTACTGTT+AGG 0.400025 contig349end:+6389 MsG0080048532.01.T01:CDS
CCTATGTTGCTTTCTGGTCC+CGG 0.400985 contig349end:-6317 None:intergenic
CACTAGCGTTATGTAGAGAT+TGG 0.401453 contig349end:-6285 None:intergenic
GAACTCGCAAAGAAAGCCTT+AGG 0.405998 contig349end:-5693 None:intergenic
GGGTTTCCTCCTTCTCTTTG+CGG 0.418532 contig349end:-6558 None:intergenic
ACGCTAGTGAGGGGTATTCC+GGG 0.429808 contig349end:+6299 MsG0080048532.01.T01:CDS
AAGCAGAAGAAGCAGGTTGA+TGG 0.435151 contig349end:-6612 None:intergenic
GATTGATGGATCCAATGTTA+AGG 0.444796 contig349end:+3785 None:intergenic
TTCGGAGGGGAAGGGAATGG+AGG 0.445877 contig349end:+5713 MsG0080048532.01.T01:CDS
ACTAGCGTTATGTAGAGATT+GGG 0.460548 contig349end:-6284 None:intergenic
CTAAATGATTAATCTCATAC+TGG 0.463913 contig349end:-3894 None:intergenic
ATGGAACACACCTACTGTTA+GGG 0.464145 contig349end:+6390 MsG0080048532.01.T01:CDS
GTTAGTTGTTGTTGAGACAG+AGG 0.465463 contig349end:+6522 MsG0080048532.01.T01:CDS
AAAGCAAATAAAACCATATG+AGG 0.477102 contig349end:-6709 None:intergenic
CTTTGCGAGTTCGGAGGGGA+AGG 0.487677 contig349end:+5704 MsG0080048532.01.T01:CDS
GCTTTCTTTGCGAGTTCGGA+GGG 0.496133 contig349end:+5699 MsG0080048532.01.T01:CDS
CAGAGCTCTTCGTCCTCCAT+TGG 0.509479 contig349end:+6582 MsG0080048532.01.T01:CDS
TGTTCCATCGCATGACAGTT+CGG 0.511917 contig349end:-6375 None:intergenic
GGCTTTCTTTGCGAGTTCGG+AGG 0.516553 contig349end:+5698 MsG0080048532.01.T01:CDS
AAAGCAACATAGGCGCACTT+TGG 0.520220 contig349end:+6327 MsG0080048532.01.T01:CDS
GGTTGATGGTTTATGACCAA+TGG 0.522943 contig349end:-6598 None:intergenic
AACAACCTAAACCTTAACAT+TGG 0.535428 contig349end:-3796 None:intergenic
AACTCGCAAAGAAAGCCTTA+GGG 0.538907 contig349end:-5692 None:intergenic
TGTATTTGATATAGGTCAAG+AGG 0.544788 contig349end:+6500 MsG0080048532.01.T01:intron
TCGGAGGGGAAGGGAATGGA+GGG 0.550463 contig349end:+5714 MsG0080048532.01.T01:CDS
GTACCCTAAGCAGAAGAAGC+AGG 0.555500 contig349end:-6619 None:intergenic
GTACAAGGCCCGCAAAGAGA+AGG 0.555978 contig349end:+6549 MsG0080048532.01.T01:CDS
GAGTTCGGAGGGGAAGGGAA+TGG 0.568645 contig349end:+5710 MsG0080048532.01.T01:CDS
TGGAGGACGAAGAGCTCTGT+GGG 0.570519 contig349end:-6578 None:intergenic
CCGGGACCAGAAAGCAACAT+AGG 0.574841 contig349end:+6317 MsG0080048532.01.T01:CDS
TTTGCGAGTTCGGAGGGGAA+GGG 0.577084 contig349end:+5705 MsG0080048532.01.T01:CDS
ACTCGCAAAGAAAGCCTTAG+GGG 0.582083 contig349end:-5691 None:intergenic
TGTTAGGGGAATGATCCAGC+AGG 0.585091 contig349end:+6405 MsG0080048532.01.T01:CDS
TGAGGCACAACCTCAAACTT+AGG 0.586101 contig349end:-6691 None:intergenic
CCATATCAGCAAACATTGCA+AGG 0.597443 contig349end:-6641 None:intergenic
GCAACCGAACTGTCATGCGA+TGG 0.602415 contig349end:+6371 MsG0080048532.01.T01:CDS
CAAGGCCCGCAAAGAGAAGG+AGG 0.604236 contig349end:+6552 MsG0080048532.01.T01:CDS
ATCTCTACATAACGCTAGTG+AGG 0.609634 contig349end:+6288 MsG0080048532.01.T01:CDS
CTAGCGTTATGTAGAGATTG+GGG 0.616077 contig349end:-6283 None:intergenic
TGGATCATTCCCCTAACAGT+AGG 0.620579 contig349end:-6400 None:intergenic
TGATGGTTTATGACCAATGG+AGG 0.622285 contig349end:-6595 None:intergenic
CAGTTCGGTTGCATTTGCCG+AGG 0.626107 contig349end:-6360 None:intergenic
TCTCTACATAACGCTAGTGA+GGG 0.627577 contig349end:+6289 MsG0080048532.01.T01:CDS
CTTTCTTTGCGAGTTCGGAG+GGG 0.630582 contig349end:+5700 MsG0080048532.01.T01:CDS
TGAGACAGAGGAGATGTACA+AGG 0.642473 contig349end:+6534 MsG0080048532.01.T01:CDS
ACTTTGGAAGCACTACGCCT+CGG 0.645701 contig349end:+6343 MsG0080048532.01.T01:CDS
TGGAACACACCTACTGTTAG+GGG 0.654422 contig349end:+6391 MsG0080048532.01.T01:CDS
ATGGAGGACGAAGAGCTCTG+TGG 0.663844 contig349end:-6579 None:intergenic
CTCGCAAAGAAAGCCTTAGG+GGG 0.664260 contig349end:-5690 None:intergenic
GGTGTCGACGTGTAAGTGTG+AGG 0.666388 contig349end:-3922 None:intergenic
CTCTACATAACGCTAGTGAG+GGG 0.670443 contig349end:+6290 MsG0080048532.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTTAAATAAAATTGGA+AGG - contig349end:6098-6117 None:intergenic 10.0%
!! TTTAAATGATTGATATAATA+TGG + contig349end:4856-4875 MsG0080048532.01.T01:intron 10.0%
!!! TTATGCTTTTATTAATTCAA+GGG + contig349end:3822-3841 MsG0080048532.01.T01:CDS 15.0%
!!! TTTTTTAGTAATAAGTCATA+AGG + contig349end:5512-5531 MsG0080048532.01.T01:intron 15.0%
!!! TTTTTTTTTTTTACTATAGC+TGG + contig349end:4063-4082 MsG0080048532.01.T01:intron 15.0%
!! AAATAAAAAAATCAAGAACG+TGG - contig349end:5339-5358 None:intergenic 20.0%
!! TTAAAAAAAAATCAAGAACG+TGG - contig349end:5402-5421 None:intergenic 20.0%
!!! AAATAAACAACTCCATTTTA+TGG - contig349end:5078-5097 None:intergenic 20.0%
!!! ATAATGAGAATTTTATTCCT+AGG - contig349end:5616-5635 None:intergenic 20.0%
!!! ATTTTTATTCCCATGAAATA+GGG + contig349end:5282-5301 MsG0080048532.01.T01:intron 20.0%
!!! GTTATGCTTTTATTAATTCA+AGG + contig349end:3821-3840 MsG0080048532.01.T01:CDS 20.0%
!!! TAAAATGGAGTTGTTTATTT+TGG + contig349end:5078-5097 MsG0080048532.01.T01:intron 20.0%
!!! TATGTTAATTTTTACAACCT+GGG - contig349end:5482-5501 None:intergenic 20.0%
!!! TTATGTTAATTTTTACAACC+TGG - contig349end:5483-5502 None:intergenic 20.0%
! AAAGCAAATAAAACCATATG+AGG - contig349end:6712-6731 None:intergenic 25.0%
! ACAGTTTATCAATTTCAGTT+TGG + contig349end:6820-6839 MsG0080048532.01.T01:three_prime_UTR 25.0%
! ATAAAAATGACGAGAATGTA+AGG - contig349end:5270-5289 None:intergenic 25.0%
! CTAAATGATTAATCTCATAC+TGG - contig349end:3897-3916 None:intergenic 25.0%
! GAACTAAAAAATTGTATTGG+AGG + contig349end:5842-5861 MsG0080048532.01.T01:intron 25.0%
! GGTAACTTTGTATTTGATAT+AGG + contig349end:6492-6511 MsG0080048532.01.T01:intron 25.0%
! TAAAAAATTGTATTGGAGGA+GGG + contig349end:5846-5865 MsG0080048532.01.T01:intron 25.0%
! TAATGAAGAAAACAAAAGCA+AGG - contig349end:4173-4192 None:intergenic 25.0%
! TAGTATATACTGAAACTGAA+AGG - contig349end:4448-4467 None:intergenic 25.0%
! TATATACTATCGTTGAATAG+CGG + contig349end:4459-4478 MsG0080048532.01.T01:intron 25.0%
! TCTTAATATAACAATCCGAT+CGG + contig349end:4982-5001 MsG0080048532.01.T01:intron 25.0%
!! AAATTCTCATTATGTTTTCC+TGG + contig349end:5623-5642 MsG0080048532.01.T01:intron 25.0%
!! AATTCTCATTATGTTTTCCT+GGG + contig349end:5624-5643 MsG0080048532.01.T01:intron 25.0%
!! ACACTTTTCTTAAAAATCCT+AGG + contig349end:5596-5615 MsG0080048532.01.T01:intron 25.0%
!! CATTTTCTTAAATTACTGTC+AGG - contig349end:3946-3965 None:intergenic 25.0%
!! CATTTTTATTCCCATGAAAT+AGG + contig349end:5281-5300 MsG0080048532.01.T01:intron 25.0%
!! TTTAACATAACATCGATTGA+TGG + contig349end:4375-4394 MsG0080048532.01.T01:intron 25.0%
!!! AATTTTTTAGTTCCCCAAAT+GGG - contig349end:5835-5854 None:intergenic 25.0%
!!! GGATTTTTAAGAAAAGTGTT+TGG - contig349end:5595-5614 None:intergenic 25.0%
!!! GTATTTTTTGAAATGTCATG+AGG + contig349end:4941-4960 MsG0080048532.01.T01:intron 25.0%
AAAAAAATCAAGAACGTGGT+TGG - contig349end:5398-5417 None:intergenic 30.0%
AAAACTCAAAACTAGTGTGA+GGG + contig349end:5021-5040 MsG0080048532.01.T01:intron 30.0%
AAATTGGAAGGAAAACACTT+TGG - contig349end:6086-6105 None:intergenic 30.0%
AACAACCTAAACCTTAACAT+TGG - contig349end:3799-3818 None:intergenic 30.0%
AATACCTATGTTTGGTAGAA+AGG + contig349end:5196-5215 MsG0080048532.01.T01:intron 30.0%
ACCAATGTTGTTAGTATACA+GGG - contig349end:6475-6494 None:intergenic 30.0%
AGAGATAAAAAACACATTCC+TGG + contig349end:5152-5171 MsG0080048532.01.T01:intron 30.0%
CTAAAAAATTGTATTGGAGG+AGG + contig349end:5845-5864 MsG0080048532.01.T01:intron 30.0%
GAAAGAAGAATACCTATGTT+TGG + contig349end:5188-5207 MsG0080048532.01.T01:intron 30.0%
GAGATAAAAAACACATTCCT+GGG + contig349end:5153-5172 MsG0080048532.01.T01:intron 30.0%
GGAACAAAAATAATATGCCT+AGG + contig349end:5217-5236 MsG0080048532.01.T01:intron 30.0%
GGGGAACTAAAAAATTGTAT+TGG + contig349end:5839-5858 MsG0080048532.01.T01:intron 30.0%
TACCAATGTTGTTAGTATAC+AGG - contig349end:6476-6495 None:intergenic 30.0%
TCTTCATTAAAACTTCAGCA+AGG + contig349end:4184-4203 MsG0080048532.01.T01:intron 30.0%
TGGAAAAAATAGAGGACATT+GGG + contig349end:5761-5780 MsG0080048532.01.T01:intron 30.0%
TGGGAACACAATAGTAAAAT+TGG - contig349end:5463-5482 None:intergenic 30.0%
TGTATTTGATATAGGTCAAG+AGG + contig349end:6500-6519 MsG0080048532.01.T01:intron 30.0%
! AATTTTACTATTGTGTTCCC+AGG + contig349end:5462-5481 MsG0080048532.01.T01:intron 30.0%
! ACTTAGGCATTTGAAGATTT+TGG - contig349end:6678-6697 None:intergenic 30.0%
! ACTTCTTTGAAAAGAATCCT+AGG + contig349end:5554-5573 MsG0080048532.01.T01:intron 30.0%
! AGGATTCTTTTCAAAGAAGT+GGG - contig349end:5554-5573 None:intergenic 30.0%
! AGTACAACTTTTAGTATGGA+AGG + contig349end:4749-4768 MsG0080048532.01.T01:intron 30.0%
! ATGAGTTTTTATCAAGAACG+TGG - contig349end:5434-5453 None:intergenic 30.0%
! ATGCATCAGTTGTCAATTTT+AGG + contig349end:4723-4742 MsG0080048532.01.T01:intron 30.0%
! CTAATCATATGTGTTGATGT+TGG + contig349end:3978-3997 MsG0080048532.01.T01:intron 30.0%
! GGTTGTCAATTTTTATTCCT+AGG - contig349end:5574-5593 None:intergenic 30.0%
! TAGGATTCTTTTCAAAGAAG+TGG - contig349end:5555-5574 None:intergenic 30.0%
! TGAACCAATTTAAAACCGAT+CGG - contig349end:5000-5019 None:intergenic 30.0%
! TGCTTTTATTAATTCAAGGG+AGG + contig349end:3825-3844 MsG0080048532.01.T01:CDS 30.0%
!! ATGTTAAGGTTTAGGTTGTT+TGG + contig349end:3799-3818 MsG0080048532.01.T01:CDS 30.0%
!! GCTTTTGTTGTGCTATATTT+CGG + contig349end:4312-4331 MsG0080048532.01.T01:intron 30.0%
!! TTGTTTTTCAGTTAGTTGTC+AGG + contig349end:4669-4688 MsG0080048532.01.T01:intron 30.0%
!!! AATTTGCTGCCTATGTTTTA+TGG - contig349end:4033-4052 None:intergenic 30.0%
!!! ATTTTTTAGTTCCCCAAATG+GGG - contig349end:5834-5853 None:intergenic 30.0%
!!! CAATTTTTTAGTTCCCCAAA+TGG - contig349end:5836-5855 None:intergenic 30.0%
AAAAAATAGAGGACATTGGG+AGG + contig349end:5764-5783 MsG0080048532.01.T01:intron 35.0%
AAACTCAAAACTAGTGTGAG+GGG + contig349end:5022-5041 MsG0080048532.01.T01:intron 35.0%
AATATAAAACCCTCCCCATT+TGG + contig349end:5818-5837 MsG0080048532.01.T01:intron 35.0%
ACTAGCGTTATGTAGAGATT+GGG - contig349end:6287-6306 None:intergenic 35.0%
ATATAAAACCCTCCCCATTT+GGG + contig349end:5819-5838 MsG0080048532.01.T01:intron 35.0%
ATGTAAGGTAAGTAGATTCC+TGG - contig349end:5255-5274 None:intergenic 35.0%
CAAAACTCAAAACTAGTGTG+AGG + contig349end:5020-5039 MsG0080048532.01.T01:intron 35.0%
CCAATTTAAGCATATGTTGC+AGG - contig349end:4259-4278 None:intergenic 35.0%
CCTGCAACATATGCTTAAAT+TGG + contig349end:4256-4275 MsG0080048532.01.T01:intron 35.0%
GAAAAAGATGAAGGAGTAAG+TGG + contig349end:5741-5760 MsG0080048532.01.T01:intron 35.0%
GATGGGAAAATTATATTCCC+AGG + contig349end:5304-5323 MsG0080048532.01.T01:intron 35.0%
GGAGATAGGTAGTTTCAAAA+TGG - contig349end:6454-6473 None:intergenic 35.0%
GGAGTAAGTGGAAAAAATAG+AGG + contig349end:5753-5772 MsG0080048532.01.T01:intron 35.0%
GTGGAAAAAATAGAGGACAT+TGG + contig349end:5760-5779 MsG0080048532.01.T01:intron 35.0%
TATTCCCATGAAATAGGGAT+GGG + contig349end:5287-5306 MsG0080048532.01.T01:intron 35.0%
TCCCTGTATACTAACAACAT+TGG + contig349end:6471-6490 MsG0080048532.01.T01:intron 35.0%
TCTTCAAATGCCTAAGTTTG+AGG + contig349end:6681-6700 MsG0080048532.01.T01:three_prime_UTR 35.0%
TGGATCCAATGTTAAGGTTT+AGG + contig349end:3791-3810 MsG0080048532.01.T01:CDS 35.0%
TGTAAGGTAAGTAGATTCCT+GGG - contig349end:5254-5273 None:intergenic 35.0%
TGTTCCTTTCTACCAAACAT+AGG - contig349end:5203-5222 None:intergenic 35.0%
TTATGGACACTTCTGATTGA+AGG - contig349end:4016-4035 None:intergenic 35.0%
TTATTCCCATGAAATAGGGA+TGG + contig349end:5286-5305 MsG0080048532.01.T01:intron 35.0%
TTGGTTCAGTTAATTGAACC+AGG + contig349end:5097-5116 MsG0080048532.01.T01:intron 35.0%
TTGTTAGTATACAGGGAGAT+AGG - contig349end:6468-6487 None:intergenic 35.0%
! AACAACTCCATTTTATGGTC+CGG - contig349end:5073-5092 None:intergenic 35.0%
! ATTCTTTTCAAAGAAGTGGG+TGG - contig349end:5551-5570 None:intergenic 35.0%
! ATTTTCCCATCCCTATTTCA+TGG - contig349end:5295-5314 None:intergenic 35.0%
! GTGCAGTACAACTTTTAGTA+TGG + contig349end:4745-4764 MsG0080048532.01.T01:intron 35.0%
! GTTTTTATCAAGAACGTGGT+TGG - contig349end:5430-5449 None:intergenic 35.0%
! TTTTCCCATCCCTATTTCAT+GGG - contig349end:5294-5313 None:intergenic 35.0%
!! CAGAAGTGTCCATAAAACAT+AGG + contig349end:4021-4040 MsG0080048532.01.T01:intron 35.0%
!! CTCTGTCATCTTTTGTTTAG+TGG + contig349end:4290-4309 MsG0080048532.01.T01:intron 35.0%
!! TAAACTGTTTTCAAGCACAG+AGG - contig349end:6808-6827 None:intergenic 35.0%
!!! CAATCCGATCGGTTTTAAAT+TGG + contig349end:4993-5012 MsG0080048532.01.T01:intron 35.0%
AACTCGCAAAGAAAGCCTTA+GGG - contig349end:5695-5714 None:intergenic 40.0%
ACATTCCTGGGAATAGAGAT+TGG + contig349end:5165-5184 MsG0080048532.01.T01:intron 40.0%
AGATTGGGGCTATAAGCAAT+TGG - contig349end:6272-6291 None:intergenic 40.0%
AGGCATGAACCAATCTCTAA+GGG + contig349end:5117-5136 MsG0080048532.01.T01:intron 40.0%
ATCTCTACATAACGCTAGTG+AGG + contig349end:6288-6307 MsG0080048532.01.T01:CDS 40.0%
ATGGAACACACCTACTGTTA+GGG + contig349end:6390-6409 MsG0080048532.01.T01:CDS 40.0%
CACTAGCGTTATGTAGAGAT+TGG - contig349end:6288-6307 None:intergenic 40.0%
CATTCCTGGGAATAGAGATT+GGG + contig349end:5166-5185 MsG0080048532.01.T01:intron 40.0%
CCATATCAGCAAACATTGCA+AGG - contig349end:6644-6663 None:intergenic 40.0%
CCTAGGAATAATTAATGCCC+AGG + contig349end:5234-5253 MsG0080048532.01.T01:intron 40.0%
CCTGGGCATTAATTATTCCT+AGG - contig349end:5237-5256 None:intergenic 40.0%
CTAGCGTTATGTAGAGATTG+GGG - contig349end:6286-6305 None:intergenic 40.0%
CTGTGGGGTGAGAAATATAT+GGG - contig349end:6024-6043 None:intergenic 40.0%
CTGTTCTGACTTCAACTTGT+AGG + contig349end:6206-6225 MsG0080048532.01.T01:intron 40.0%
GAGGGTTTGGAAAAAGATGA+AGG + contig349end:5732-5751 MsG0080048532.01.T01:CDS 40.0%
GATTGGGGCTATAAGCAATT+GGG - contig349end:6271-6290 None:intergenic 40.0%
TAAGGCTTTCTTTGCGAGTT+CGG + contig349end:5695-5714 MsG0080048532.01.T01:CDS 40.0%
TACAGTGACACCAAACATCA+CGG - contig349end:4901-4920 None:intergenic 40.0%
TATAAAACCCTCCCCATTTG+GGG + contig349end:5820-5839 MsG0080048532.01.T01:intron 40.0%
TCCTGGGAAAGTTAATTCCA+GGG + contig349end:5640-5659 MsG0080048532.01.T01:intron 40.0%
TCGCATATTGCAGAAAAGAG+CGG - contig349end:4613-4632 None:intergenic 40.0%
TCTCTACATAACGCTAGTGA+GGG + contig349end:6289-6308 MsG0080048532.01.T01:CDS 40.0%
TCTGTGGGGTGAGAAATATA+TGG - contig349end:6025-6044 None:intergenic 40.0%
TGATGGTTTATGACCAATGG+AGG - contig349end:6598-6617 None:intergenic 40.0%
TTCCTGGGAAAGTTAATTCC+AGG + contig349end:5639-5658 MsG0080048532.01.T01:intron 40.0%
TTGTGAAGTAGCTGCTATAG+CGG + contig349end:4551-4570 MsG0080048532.01.T01:intron 40.0%
TTTGGCATTGTTTGCGAGTT+TGG - contig349end:6068-6087 None:intergenic 40.0%
! ATTGTATTGGAGGAGGGTTT+TGG + contig349end:5852-5871 MsG0080048532.01.T01:intron 40.0%
! CCTTGCAATGTTTGCTGATA+TGG + contig349end:6641-6660 MsG0080048532.01.T01:three_prime_UTR 40.0%
! GAAAGCATAACCGTGATGTT+TGG + contig349end:4888-4907 MsG0080048532.01.T01:intron 40.0%
! GAAGGCATAACTGTGATGTT+TGG + contig349end:4767-4786 MsG0080048532.01.T01:intron 40.0%
! GTTAGTTGTTGTTGAGACAG+AGG + contig349end:6522-6541 MsG0080048532.01.T01:CDS 40.0%
! TTGTATTGGAGGAGGGTTTT+GGG + contig349end:5853-5872 MsG0080048532.01.T01:intron 40.0%
!! GGTTGATGGTTTATGACCAA+TGG - contig349end:6601-6620 None:intergenic 40.0%
AAAGCAACATAGGCGCACTT+TGG + contig349end:6327-6346 MsG0080048532.01.T01:CDS 45.0%
ACTCGCAAAGAAAGCCTTAG+GGG - contig349end:5694-5713 None:intergenic 45.0%
CAACCTGCTTCTTCTGCTTA+GGG + contig349end:6616-6635 MsG0080048532.01.T01:CDS 45.0%
CAGGCATGAACCAATCTCTA+AGG + contig349end:5116-5135 MsG0080048532.01.T01:intron 45.0%
CATGAGCATGAATGCGTTCA+AGG + contig349end:6229-6248 MsG0080048532.01.T01:intron 45.0%
CATTGTTTGCGAGTTTGGAG+GGG - contig349end:6063-6082 None:intergenic 45.0%
CTCTACATAACGCTAGTGAG+GGG + contig349end:6290-6309 MsG0080048532.01.T01:CDS 45.0%
CTTTCCCAATCTCTATTCCC+AGG - contig349end:5173-5192 None:intergenic 45.0%
GAACTCGCAAAGAAAGCCTT+AGG - contig349end:5696-5715 None:intergenic 45.0%
GATGGAACACACCTACTGTT+AGG + contig349end:6389-6408 MsG0080048532.01.T01:CDS 45.0%
GCATTGTTTGCGAGTTTGGA+GGG - contig349end:6064-6083 None:intergenic 45.0%
GCTATAAGCAATTGGGACAC+AGG - contig349end:6264-6283 None:intergenic 45.0%
GGCATGAACCAATCTCTAAG+GGG + contig349end:5118-5137 MsG0080048532.01.T01:intron 45.0%
TCAACCTGCTTCTTCTGCTT+AGG + contig349end:6615-6634 MsG0080048532.01.T01:CDS 45.0%
TCCCTGGAATTAACTTTCCC+AGG - contig349end:5644-5663 None:intergenic 45.0%
TGAGACAGAGGAGATGTACA+AGG + contig349end:6534-6553 MsG0080048532.01.T01:CDS 45.0%
TGAGGCACAACCTCAAACTT+AGG - contig349end:6694-6713 None:intergenic 45.0%
TGGAACACACCTACTGTTAG+GGG + contig349end:6391-6410 MsG0080048532.01.T01:CDS 45.0%
TGGATCATTCCCCTAACAGT+AGG - contig349end:6403-6422 None:intergenic 45.0%
TGTGCTATATTTCGGCAAGC+AGG + contig349end:4320-4339 MsG0080048532.01.T01:intron 45.0%
TGTTCCATCGCATGACAGTT+CGG - contig349end:6378-6397 None:intergenic 45.0%
! AAGCAGAAGAAGCAGGTTGA+TGG - contig349end:6615-6634 None:intergenic 45.0%
! CAGGGAGTAATTTTGCATCC+GGG + contig349end:5658-5677 MsG0080048532.01.T01:intron 45.0%
! CCAGGGAGTAATTTTGCATC+CGG + contig349end:5657-5676 MsG0080048532.01.T01:intron 45.0%
! CTTAGAGATTGGTTCATGCC+TGG - contig349end:5118-5137 None:intergenic 45.0%
! CTTCTGATTGAAGGCGTGTA+CGG - contig349end:4007-4026 None:intergenic 45.0%
! GCATGAACCAATCTCTAAGG+GGG + contig349end:5119-5138 MsG0080048532.01.T01:intron 45.0%
! GTTTGAGGTTGTGCCTCATA+TGG + contig349end:6696-6715 MsG0080048532.01.T01:three_prime_UTR 45.0%
! TGTATTGGAGGAGGGTTTTG+GGG + contig349end:5854-5873 MsG0080048532.01.T01:intron 45.0%
! TTTAGTTCCCCAAATGGGGA+GGG - contig349end:5830-5849 None:intergenic 45.0%
!!! TTTTAGTTCCCCAAATGGGG+AGG - contig349end:5831-5850 None:intergenic 45.0%
!! AGATAAAATTTAAATAAAAT+TGG - contig349end:6102-6121 None:intergenic 5.0%
!! CAATATATTATATATAAATA+TGG + contig349end:4637-4656 MsG0080048532.01.T01:intron 5.0%
ACAAACGCCCCCTTAGAGAT+TGG - contig349end:5129-5148 None:intergenic 50.0%
ATAGAGGACATTGGGAGGAG+AGG + contig349end:5769-5788 MsG0080048532.01.T01:intron 50.0%
ATGGCTCAGTTTGCGAAAGC+CGG + contig349end:5051-5070 MsG0080048532.01.T01:intron 50.0%
CCGGATGCAAAATTACTCCC+TGG - contig349end:5660-5679 None:intergenic 50.0%
CTAGTGTGAGGGGAGGAAAA+TGG + contig349end:5032-5051 MsG0080048532.01.T01:intron 50.0%
CTCAAAACTAGTGTGAGGGG+AGG + contig349end:5025-5044 MsG0080048532.01.T01:intron 50.0%
CTCGCAAAGAAAGCCTTAGG+GGG - contig349end:5693-5712 None:intergenic 50.0%
CTTTCTTTGCGAGTTCGGAG+GGG + contig349end:5700-5719 MsG0080048532.01.T01:CDS 50.0%
GATCTTGTGCTGAAACCTGC+TGG - contig349end:6423-6442 None:intergenic 50.0%
GCGAAAGCCGGACCATAAAA+TGG + contig349end:5063-5082 MsG0080048532.01.T01:intron 50.0%
GCTTTCTTTGCGAGTTCGGA+GGG + contig349end:5699-5718 MsG0080048532.01.T01:CDS 50.0%
GGCATTGTTTGCGAGTTTGG+AGG - contig349end:6065-6084 None:intergenic 50.0%
GGGTTTCCTCCTTCTCTTTG+CGG - contig349end:6561-6580 None:intergenic 50.0%
GGTTTCCTCCTTCTCTTTGC+GGG - contig349end:6560-6579 None:intergenic 50.0%
GTACCCTAAGCAGAAGAAGC+AGG - contig349end:6622-6641 None:intergenic 50.0%
TAGAGGACATTGGGAGGAGA+GGG + contig349end:5770-5789 MsG0080048532.01.T01:intron 50.0%
TGTTAGGGGAATGATCCAGC+AGG + contig349end:6405-6424 MsG0080048532.01.T01:CDS 50.0%
TTTGCGAGTTTGGAGGGGAA+GGG - contig349end:6058-6077 None:intergenic 50.0%
! AGAACGTGGCACTCTTTTCC+TGG - contig349end:5325-5344 None:intergenic 50.0%
! GAACGTGGCACTCTTTTCCT+GGG - contig349end:5324-5343 None:intergenic 50.0%
!! AACGCTAGTGAGGGGTATTC+CGG + contig349end:6298-6317 MsG0080048532.01.T01:CDS 50.0%
!! ACTTTGGAAGCACTACGCCT+CGG + contig349end:6343-6362 MsG0080048532.01.T01:CDS 50.0%
!! AGTGCGCCTATGTTGCTTTC+TGG - contig349end:6326-6345 None:intergenic 50.0%
!! CCTATGTTGCTTTCTGGTCC+CGG - contig349end:6320-6339 None:intergenic 50.0%
!! CTTAGGGGGTGTTTGTTTCC+CGG - contig349end:5679-5698 None:intergenic 50.0%
!! GTATTGGAGGAGGGTTTTGG+GGG + contig349end:5855-5874 MsG0080048532.01.T01:intron 50.0%
!!! TATTGGAGGAGGGTTTTGGG+GGG + contig349end:5856-5875 MsG0080048532.01.T01:intron 50.0%
AAGGGAAGGAAGGGCTCTGT+GGG - contig349end:6040-6059 None:intergenic 55.0%
ATGGAGGACGAAGAGCTCTG+TGG - contig349end:6582-6601 None:intergenic 55.0%
CAGAGCTCTTCGTCCTCCAT+TGG + contig349end:6582-6601 MsG0080048532.01.T01:CDS 55.0%
CAGTTCGGTTGCATTTGCCG+AGG - contig349end:6363-6382 None:intergenic 55.0%
CCGGGACCAGAAAGCAACAT+AGG + contig349end:6317-6336 MsG0080048532.01.T01:CDS 55.0%
CGGGAAACAAACACCCCCTA+AGG + contig349end:5677-5696 MsG0080048532.01.T01:CDS 55.0%
GCAACCGAACTGTCATGCGA+TGG + contig349end:6371-6390 MsG0080048532.01.T01:CDS 55.0%
GCAAGCAGGTCTGAATCTGC+TGG + contig349end:4334-4353 MsG0080048532.01.T01:intron 55.0%
GGCTTTCTTTGCGAGTTCGG+AGG + contig349end:5698-5717 MsG0080048532.01.T01:CDS 55.0%
GTACAAGGCCCGCAAAGAGA+AGG + contig349end:6549-6568 MsG0080048532.01.T01:CDS 55.0%
GTTTGCGAGTTTGGAGGGGA+AGG - contig349end:6059-6078 None:intergenic 55.0%
TGGAGGACGAAGAGCTCTGT+GGG - contig349end:6581-6600 None:intergenic 55.0%
TTGGAGGGGAAGGGAAGGAA+GGG - contig349end:6049-6068 None:intergenic 55.0%
TTTGCGAGTTCGGAGGGGAA+GGG + contig349end:5705-5724 MsG0080048532.01.T01:CDS 55.0%
TTTGGAGGGGAAGGGAAGGA+AGG - contig349end:6050-6069 None:intergenic 55.0%
! TTGGGAGGAGAGGGCTTTGA+CGG + contig349end:5779-5798 MsG0080048532.01.T01:intron 55.0%
!! ACGCTAGTGAGGGGTATTCC+GGG + contig349end:6299-6318 MsG0080048532.01.T01:CDS 55.0%
!! GGTGTCGACGTGTAAGTGTG+AGG - contig349end:3925-3944 None:intergenic 55.0%
!!! AGGAGGGTTTTGGGGGGTTT+GGG + contig349end:5862-5881 MsG0080048532.01.T01:intron 55.0%
AGGGAAGGAAGGGCTCTGTG+GGG - contig349end:6039-6058 None:intergenic 60.0%
CAAGGCCCGCAAAGAGAAGG+AGG + contig349end:6552-6571 MsG0080048532.01.T01:CDS 60.0%
CGAGTTTGGAGGGGAAGGGA+AGG - contig349end:6054-6073 None:intergenic 60.0%
CTTTGCGAGTTCGGAGGGGA+AGG + contig349end:5704-5723 MsG0080048532.01.T01:CDS 60.0%
GAAGGGAAGGAAGGGCTCTG+TGG - contig349end:6041-6060 None:intergenic 60.0%
GAGTTCGGAGGGGAAGGGAA+TGG + contig349end:5710-5729 MsG0080048532.01.T01:CDS 60.0%
GGGGAAGGGAATGGAGGGTT+TGG + contig349end:5719-5738 MsG0080048532.01.T01:CDS 60.0%
TCGGAGGGGAAGGGAATGGA+GGG + contig349end:5714-5733 MsG0080048532.01.T01:CDS 60.0%
TTCGGAGGGGAAGGGAATGG+AGG + contig349end:5713-5732 MsG0080048532.01.T01:CDS 60.0%
! TGGGAGGAGAGGGCTTTGAC+GGG + contig349end:5780-5799 MsG0080048532.01.T01:intron 60.0%
!!! AGGGTTTTGGGGGGTTTGGG+AGG + contig349end:5865-5884 MsG0080048532.01.T01:intron 60.0%
!!! GAGGAGGGTTTTGGGGGGTT+TGG + contig349end:5861-5880 MsG0080048532.01.T01:intron 60.0%
!!! GGGTTTTGGGGGGTTTGGGA+GGG + contig349end:5866-5885 MsG0080048532.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
contig349end gene 3790 6872 3790 ID=MsG0080048532.01;Name=MsG0080048532.01
contig349end mRNA 3790 6872 3790 ID=MsG0080048532.01.T01;Parent=MsG0080048532.01;Name=MsG0080048532.01.T01;_AED=0.49;_eAED=0.66;_QI=0|0.33|0.25|1|1|1|4|236|173
contig349end exon 6514 6872 6514 ID=MsG0080048532.01.T01:exon:17172;Parent=MsG0080048532.01.T01
contig349end exon 6283 6426 6283 ID=MsG0080048532.01.T01:exon:17171;Parent=MsG0080048532.01.T01
contig349end exon 5661 5755 5661 ID=MsG0080048532.01.T01:exon:17170;Parent=MsG0080048532.01.T01
contig349end exon 3790 3949 3790 ID=MsG0080048532.01.T01:exon:17169;Parent=MsG0080048532.01.T01
contig349end CDS 3790 3949 3790 ID=MsG0080048532.01.T01:cds;Parent=MsG0080048532.01.T01
contig349end CDS 5661 5755 5661 ID=MsG0080048532.01.T01:cds;Parent=MsG0080048532.01.T01
contig349end CDS 6283 6426 6283 ID=MsG0080048532.01.T01:cds;Parent=MsG0080048532.01.T01
contig349end CDS 6514 6636 6514 ID=MsG0080048532.01.T01:cds;Parent=MsG0080048532.01.T01
contig349end three_prime_UTR 6637 6872 6637 ID=MsG0080048532.01.T01:three_prime_utr;Parent=MsG0080048532.01.T01
Gene Sequence

>MsG0080048532.01.T01

ATGGATCCAATGTTAAGGTTTAGGTTGTTTGGTTATGCTTTTATTAATTCAAGGGAGGAAGATGAGTTAACAAAAAGAATGTTGTTGCTTAATCTAATTGTTCTCCAGTATGAGATTAATCATTTAGAATTACCTCACACTTACACGTCGACACCTGACAGGAGTAATTTTGCATCCGGGAAACAAACACCCCCTAAGGCTTTCTTTGCGAGTTCGGAGGGGAAGGGAATGGAGGGTTTGGAAAAAGATGAAGGACCCCAATCTCTACATAACGCTAGTGAGGGGTATTCCGGGACCAGAAAGCAACATAGGCGCACTTTGGAAGCACTACGCCTCGGCAAATGCAACCGAACTGTCATGCGATGGAACACACCTACTGTTAGGGGAATGATCCAGCAGGTCAAGAGGTTAGTTGTTGTTGAGACAGAGGAGATGTACAAGGCCCGCAAAGAGAAGGAGGAAACCCACAGAGCTCTTCGTCCTCCATTGGTCATAAACCATCAACCTGCTTCTTCTGCTTAG
Protein sequence

>MsG0080048532.01.T01

MDPMLRFRLFGYAFINSREEDELTKRMLLLNLIVLQYEINHLELPHTYTSTPDRSNFASGKQTPPKAFFASSEGKGMEGLEKDEGPQSLHNASEGYSGTRKQHRRTLEALRLGKCNRTVMRWNTPTVRGMIQQVKRLVVVETEEMYKARKEKEETHRALRPPLVINHQPASSA*