Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015822.01.T01 | XP_013461022.1 | 97.872 | 94 | 2 | 0 | 1 | 94 | 50 | 143 | 8.55E-62 | 194 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015822.01.T01 | I3T405 | 97.872 | 94 | 2 | 0 | 1 | 94 | 50 | 143 | 4.08e-62 | 194 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280006422.01 | MsG0380015822.01 | 0.809397 | 1.930925e-50 | 1.132497e-47 |
| MsG0380014647.01 | MsG0380015822.01 | 0.805832 | 1.103599e-49 | 5.898135e-47 |
| MsG0380015400.01 | MsG0380015822.01 | 0.818476 | 1.921810e-52 | 1.440127e-49 |
| MsG0380015770.01 | MsG0380015822.01 | 0.815560 | 8.683652e-52 | 6.007086e-49 |
| MsG0380015822.01 | MsG0380016886.01 | 0.814773 | 1.299055e-51 | 8.795140e-49 |
| MsG0380015822.01 | MsG0480018906.01 | 0.803755 | 2.995632e-49 | 1.517722e-46 |
| MsG0380015822.01 | MsG0480021448.01 | 0.850903 | 1.256433e-60 | 2.498961e-57 |
| MsG0380015822.01 | MsG0480021451.01 | 0.801469 | 8.871965e-49 | 4.240254e-46 |
| MsG0380015822.01 | MsG0480021453.01 | 0.830392 | 3.013463e-55 | 3.170736e-52 |
| MsG0380015822.01 | MsG0480021464.01 | 0.810381 | 1.185638e-50 | 7.135878e-48 |
| MsG0380015822.01 | MsG0580026047.01 | 0.835033 | 2.123785e-56 | 2.565529e-53 |
| MsG0380015822.01 | MsG0580027344.01 | 0.819888 | 9.164924e-53 | 7.141952e-50 |
| MsG0380015822.01 | MsG0680032430.01 | 0.809498 | 1.836556e-50 | 1.080034e-47 |
| MsG0380015822.01 | MsG0780038911.01 | 0.823274 | 1.513109e-53 | 1.296798e-50 |
| MsG0380015822.01 | MsG0780039841.01 | 0.813594 | 2.365023e-51 | 1.551083e-48 |
| MsG0380015822.01 | MsG0880043465.01 | 0.841685 | 4.090864e-58 | 6.056732e-55 |
| MsG0380015822.01 | MsG0880047191.01 | 0.819142 | 1.356743e-52 | 1.035430e-49 |
| MsG0280008417.01 | MsG0380015822.01 | 0.800168 | 1.635915e-48 | 7.564934e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015822.01.T01 | MTR_3g078240 | 97.872 | 94 | 2 | 0 | 1 | 94 | 50 | 143 | 1.04e-65 | 194 |
| MsG0380015822.01.T01 | MTR_4g106650 | 63.889 | 72 | 20 | 2 | 2 | 73 | 67 | 132 | 2.68e-25 | 92.4 |
| MsG0380015822.01.T01 | MTR_6g007727 | 52.222 | 90 | 39 | 3 | 1 | 90 | 69 | 154 | 2.48e-21 | 82.4 |
| MsG0380015822.01.T01 | MTR_2g461190 | 45.333 | 75 | 35 | 3 | 2 | 76 | 66 | 134 | 7.90e-14 | 62.8 |
| MsG0380015822.01.T01 | MTR_7g045010 | 90.625 | 32 | 3 | 0 | 63 | 94 | 46 | 77 | 3.86e-13 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015822.01.T01 | AT5G14895 | 47.423 | 97 | 39 | 5 | 1 | 87 | 69 | 163 | 2.89e-20 | 80.1 |
| MsG0380015822.01.T01 | AT3G48020 | 50.602 | 83 | 38 | 2 | 1 | 81 | 44 | 125 | 6.27e-20 | 78.6 |
| MsG0380015822.01.T01 | AT5G62865 | 57.692 | 78 | 29 | 3 | 1 | 76 | 67 | 142 | 4.84e-19 | 77.0 |
| MsG0380015822.01.T01 | AT3G01430 | 38.938 | 113 | 38 | 5 | 2 | 88 | 70 | 177 | 1.39e-16 | 70.9 |
Find 30 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTCTGATTCGACGGATT+TGG | 0.249257 | 3:+75585211 | MsG0380015822.01.T01:CDS |
| ATGATTCTCCTGACGGGTTT+CGG | 0.304930 | 3:+75585150 | MsG0380015822.01.T01:CDS |
| CGTTTATTCGACGGTTCAAC+CGG | 0.363885 | 3:+75585030 | MsG0380015822.01.T01:CDS |
| GGAAACCACCGCTCTTGTTC+CGG | 0.390683 | 3:-75585049 | None:intergenic |
| GAGTGGTCGGAAATCGTTGC+TGG | 0.429415 | 3:+75584993 | MsG0380015822.01.T01:CDS |
| ATTTCGATGAGGGGCAGAAT+GGG | 0.456984 | 3:+75585117 | MsG0380015822.01.T01:CDS |
| AGCGGTGGTTTCCGTCATGC+GGG | 0.490593 | 3:+75585059 | MsG0380015822.01.T01:CDS |
| AACTTTGAAGTCTGATTCGA+CGG | 0.500555 | 3:+75585205 | MsG0380015822.01.T01:CDS |
| GTCGAATCAGACTTCAAAGT+TGG | 0.500856 | 3:-75585203 | None:intergenic |
| TCGAAAATGATTCTCCTGAC+GGG | 0.518747 | 3:+75585144 | MsG0380015822.01.T01:CDS |
| CATGAAAATCCGCGAGTGGT+CGG | 0.522571 | 3:+75584980 | None:intergenic |
| AATTTCGATGAGGGGCAGAA+TGG | 0.524198 | 3:+75585116 | MsG0380015822.01.T01:CDS |
| TTCGAAAATGATTCTCCTGA+CGG | 0.534748 | 3:+75585143 | MsG0380015822.01.T01:CDS |
| GAGCGGTGGTTTCCGTCATG+CGG | 0.545646 | 3:+75585058 | MsG0380015822.01.T01:CDS |
| TACTCTTTGAATTTCGATGA+GGG | 0.566198 | 3:+75585107 | MsG0380015822.01.T01:CDS |
| GGTGGAAGACGTTTATTCGA+CGG | 0.599260 | 3:+75585021 | MsG0380015822.01.T01:CDS |
| CGGAAATCGTTGCTGGACCA+CGG | 0.599286 | 3:+75585000 | MsG0380015822.01.T01:CDS |
| ATTGATACTTCCCCGCATGA+CGG | 0.603381 | 3:-75585070 | None:intergenic |
| TTACTCTTTGAATTTCGATG+AGG | 0.616940 | 3:+75585106 | MsG0380015822.01.T01:CDS |
| TTTCGATGAGGGGCAGAATG+GGG | 0.623061 | 3:+75585118 | MsG0380015822.01.T01:CDS |
| AAATTCAAAGAGTAACTCAA+AGG | 0.636783 | 3:-75585098 | None:intergenic |
| CAACGATTTCCGACCACTCG+CGG | 0.640334 | 3:-75584989 | None:intergenic |
| ACTCTTTGAATTTCGATGAG+GGG | 0.647474 | 3:+75585108 | MsG0380015822.01.T01:CDS |
| TTCAGCTCGTTACGTTGCGG+CGG | 0.650823 | 3:+75585178 | MsG0380015822.01.T01:CDS |
| CGGTTCAACCGGAACAAGAG+CGG | 0.664702 | 3:+75585041 | MsG0380015822.01.T01:CDS |
| TTCAACCGGAACAAGAGCGG+TGG | 0.667010 | 3:+75585044 | MsG0380015822.01.T01:CDS |
| GCGGTGGTTTCCGTCATGCG+GGG | 0.669488 | 3:+75585060 | MsG0380015822.01.T01:CDS |
| GATTCATGAAAATCCGCGAG+TGG | 0.686689 | 3:+75584976 | None:intergenic |
| CGAATAAACGTCTTCCACCG+TGG | 0.729057 | 3:-75585017 | None:intergenic |
| AAATCGTTGCTGGACCACGG+TGG | 0.739447 | 3:+75585003 | MsG0380015822.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTCAAAGAGTAACTCAA+AGG | - | Chr3:75585101-75585120 | None:intergenic | 25.0% |
| TACTCTTTGAATTTCGATGA+GGG | + | Chr3:75585107-75585126 | MsG0380015822.01.T01:CDS | 30.0% | |
| TTACTCTTTGAATTTCGATG+AGG | + | Chr3:75585106-75585125 | MsG0380015822.01.T01:CDS | 30.0% | |
| ACTCTTTGAATTTCGATGAG+GGG | + | Chr3:75585108-75585127 | MsG0380015822.01.T01:CDS | 35.0% | |
| TTCGAAAATGATTCTCCTGA+CGG | + | Chr3:75585143-75585162 | MsG0380015822.01.T01:CDS | 35.0% | |
| !! | AACTTTGAAGTCTGATTCGA+CGG | + | Chr3:75585205-75585224 | MsG0380015822.01.T01:CDS | 35.0% |
| GTCGAATCAGACTTCAAAGT+TGG | - | Chr3:75585206-75585225 | None:intergenic | 40.0% | |
| TCGAAAATGATTCTCCTGAC+GGG | + | Chr3:75585144-75585163 | MsG0380015822.01.T01:CDS | 40.0% | |
| !! | TTTTTCAGCTCGTTACGTTG+CGG | + | Chr3:75585175-75585194 | MsG0380015822.01.T01:CDS | 40.0% |
| AATTTCGATGAGGGGCAGAA+TGG | + | Chr3:75585116-75585135 | MsG0380015822.01.T01:CDS | 45.0% | |
| ATTGATACTTCCCCGCATGA+CGG | - | Chr3:75585073-75585092 | None:intergenic | 45.0% | |
| ATTTCGATGAGGGGCAGAAT+GGG | + | Chr3:75585117-75585136 | MsG0380015822.01.T01:CDS | 45.0% | |
| CGTTTATTCGACGGTTCAAC+CGG | + | Chr3:75585030-75585049 | MsG0380015822.01.T01:CDS | 45.0% | |
| GAAAAATTCCGAAACCCGTC+AGG | - | Chr3:75585161-75585180 | None:intergenic | 45.0% | |
| GGTGGAAGACGTTTATTCGA+CGG | + | Chr3:75585021-75585040 | MsG0380015822.01.T01:CDS | 45.0% | |
| ! | GAAGTCTGATTCGACGGATT+TGG | + | Chr3:75585211-75585230 | MsG0380015822.01.T01:CDS | 45.0% |
| !! | ATGATTCTCCTGACGGGTTT+CGG | + | Chr3:75585150-75585169 | MsG0380015822.01.T01:CDS | 45.0% |
| CGAATAAACGTCTTCCACCG+TGG | - | Chr3:75585020-75585039 | None:intergenic | 50.0% | |
| TTTCGATGAGGGGCAGAATG+GGG | + | Chr3:75585118-75585137 | MsG0380015822.01.T01:CDS | 50.0% | |
| AAATCGTTGCTGGACCACGG+TGG | + | Chr3:75585003-75585022 | MsG0380015822.01.T01:CDS | 55.0% | |
| CAACGATTTCCGACCACTCG+CGG | - | Chr3:75584992-75585011 | None:intergenic | 55.0% | |
| CGGAAATCGTTGCTGGACCA+CGG | + | Chr3:75585000-75585019 | MsG0380015822.01.T01:CDS | 55.0% | |
| CGGTTCAACCGGAACAAGAG+CGG | + | Chr3:75585041-75585060 | MsG0380015822.01.T01:CDS | 55.0% | |
| GAGTGGTCGGAAATCGTTGC+TGG | + | Chr3:75584993-75585012 | MsG0380015822.01.T01:CDS | 55.0% | |
| GGAAACCACCGCTCTTGTTC+CGG | - | Chr3:75585052-75585071 | None:intergenic | 55.0% | |
| TTCAACCGGAACAAGAGCGG+TGG | + | Chr3:75585044-75585063 | MsG0380015822.01.T01:CDS | 55.0% | |
| TTCAGCTCGTTACGTTGCGG+CGG | + | Chr3:75585178-75585197 | MsG0380015822.01.T01:CDS | 55.0% | |
| AGCGGTGGTTTCCGTCATGC+GGG | + | Chr3:75585059-75585078 | MsG0380015822.01.T01:CDS | 60.0% | |
| GAGCGGTGGTTTCCGTCATG+CGG | + | Chr3:75585058-75585077 | MsG0380015822.01.T01:CDS | 60.0% | |
| GCGGTGGTTTCCGTCATGCG+GGG | + | Chr3:75585060-75585079 | MsG0380015822.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 75584981 | 75585265 | 75584981 | ID=MsG0380015822.01;Name=MsG0380015822.01 |
| Chr3 | mRNA | 75584981 | 75585265 | 75584981 | ID=MsG0380015822.01.T01;Parent=MsG0380015822.01;Name=MsG0380015822.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|94 |
| Chr3 | exon | 75584981 | 75585265 | 75584981 | ID=MsG0380015822.01.T01:exon:1078;Parent=MsG0380015822.01.T01 |
| Chr3 | CDS | 75584981 | 75585265 | 75584981 | ID=MsG0380015822.01.T01:cds;Parent=MsG0380015822.01.T01 |
| Gene Sequence |
| Protein sequence |
