Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016196.01.T01 | XP_003601780.1 | 97.917 | 96 | 2 | 0 | 1 | 96 | 1 | 96 | 2.11E-58 | 184 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016196.01.T01 | G7JBY8 | 97.917 | 96 | 2 | 0 | 1 | 96 | 1 | 96 | 1.01e-58 | 184 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001102.01 | MsG0380016196.01 | -0.807615 | 4.634809e-50 | 2.593330e-47 |
| MsG0180004027.01 | MsG0380016196.01 | -0.800647 | 1.306253e-48 | 6.114749e-46 |
| MsG0180004559.01 | MsG0380016196.01 | -0.801078 | 1.066768e-48 | 5.048704e-46 |
| MsG0180005667.01 | MsG0380016196.01 | -0.804395 | 2.205607e-49 | 1.135916e-46 |
| MsG0380014754.01 | MsG0380016196.01 | -0.805492 | 1.300534e-49 | 6.890011e-47 |
| MsG0380016196.01 | MsG0480020597.01 | -0.815415 | 9.352915e-52 | 6.444219e-49 |
| MsG0380016196.01 | MsG0480021984.01 | -0.816273 | 6.020785e-52 | 4.247187e-49 |
| MsG0380016196.01 | MsG0480023324.01 | -0.881808 | 1.764755e-70 | 1.048632e-66 |
| MsG0380016196.01 | MsG0580028330.01 | -0.855859 | 4.753882e-62 | 1.113027e-58 |
| MsG0380016196.01 | MsG0780041363.01 | -0.809661 | 1.694883e-50 | 1.001071e-47 |
| MsG0380016196.01 | MsG0880043961.01 | -0.807874 | 4.083633e-50 | 2.300390e-47 |
| MsG0280007844.01 | MsG0380016196.01 | -0.816537 | 5.255308e-52 | 3.733840e-49 |
| MsG0280008407.01 | MsG0380016196.01 | -0.810906 | 9.129169e-51 | 5.572455e-48 |
| MsG0280009241.01 | MsG0380016196.01 | -0.803382 | 3.580119e-49 | 1.796444e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016196.01.T01 | MTR_3g085300 | 97.917 | 96 | 2 | 0 | 1 | 96 | 1 | 96 | 2.55e-62 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016196.01.T01 | AT5G43260 | 75.000 | 96 | 23 | 1 | 1 | 96 | 1 | 95 | 2.94e-48 | 149 |
Find 45 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTTAGACCGGTGGCTAAT+TGG | 0.237429 | 3:-80230784 | None:intergenic |
| CGCTGGTCTGACGGTGATAT+CGG | 0.293243 | 3:+80230923 | MsG0380016196.01.T01:CDS |
| TGTAGTACGTGTTCCGGTTC+GGG | 0.338572 | 3:+80230947 | MsG0380016196.01.T01:CDS |
| TTGTAGTACGTGTTCCGGTT+CGG | 0.355021 | 3:+80230946 | MsG0380016196.01.T01:CDS |
| TCGACCGGTTCCGTTGCAGC+TGG | 0.371451 | 3:-80230882 | None:intergenic |
| GCAACCCATGCGACCCGAAC+CGG | 0.400519 | 3:-80230960 | None:intergenic |
| ATCGGTTGTAGTACGTGTTC+CGG | 0.416543 | 3:+80230941 | MsG0380016196.01.T01:CDS |
| TGCAGCAGTTGTGGTGGGTC+CGG | 0.423170 | 3:+80230980 | MsG0380016196.01.T01:CDS |
| TGCCCCAGCTGCAACGGAAC+CGG | 0.425765 | 3:+80230878 | MsG0380016196.01.T01:CDS |
| GTGTTCCGGTTCGGGTCGCA+TGG | 0.429904 | 3:+80230955 | MsG0380016196.01.T01:CDS |
| GGTCGAATTGTGATTCTAGA+AGG | 0.439417 | 3:-80231019 | None:intergenic |
| CGGAGCGGTTCTCGTTAAAC+AGG | 0.441340 | 3:+80230814 | MsG0380016196.01.T01:CDS |
| AGTTGTGGTGGGTCCGGTAC+CGG | 0.462983 | 3:+80230986 | MsG0380016196.01.T01:CDS |
| CGACCGGTTCCGTTGCAGCT+GGG | 0.464020 | 3:-80230881 | None:intergenic |
| AGGTAAAGGTCGACCGGTAC+CGG | 0.470137 | 3:-80230999 | None:intergenic |
| CTGGGGCATCGTGGAAATGA+TGG | 0.471692 | 3:-80230863 | None:intergenic |
| CTCTGTTCACGCTGGTCTGA+CGG | 0.472755 | 3:+80230914 | MsG0380016196.01.T01:CDS |
| CGCATGGGTTGCAGCAGTTG+TGG | 0.478427 | 3:+80230971 | MsG0380016196.01.T01:CDS |
| CAACTAAGAAGGCGGTTTGT+CGG | 0.482925 | 3:-80231040 | None:intergenic |
| TCCGGCTAGGACGCTTAGAC+CGG | 0.501048 | 3:-80230795 | None:intergenic |
| GACCGGTTCCGTTGCAGCTG+GGG | 0.510274 | 3:-80230880 | None:intergenic |
| TGGAAATGATGGACCGGCCA+TGG | 0.525670 | 3:-80230852 | None:intergenic |
| CCACGATGCCCCAGCTGCAA+CGG | 0.530657 | 3:+80230872 | MsG0380016196.01.T01:CDS |
| GCATCGTGGAAATGATGGAC+CGG | 0.536537 | 3:-80230858 | None:intergenic |
| TCCCTTGCCTCTGTTCACGC+TGG | 0.541179 | 3:+80230906 | MsG0380016196.01.T01:CDS |
| ACCGGTCTAAGCGTCCTAGC+CGG | 0.549575 | 3:+80230794 | MsG0380016196.01.T01:CDS |
| ACCGAACCGGTCAACTAAGA+AGG | 0.550003 | 3:-80231051 | None:intergenic |
| TGTTCCGGTTCGGGTCGCAT+GGG | 0.561573 | 3:+80230956 | MsG0380016196.01.T01:CDS |
| ATGGGTTGCAGCAGTTGTGG+TGG | 0.567587 | 3:+80230974 | MsG0380016196.01.T01:CDS |
| TAACGAGAACCGCTCCGGCT+AGG | 0.568493 | 3:-80230808 | None:intergenic |
| CTGTTTAACGAGAACCGCTC+CGG | 0.587269 | 3:-80230813 | None:intergenic |
| TCTAGAAGGTAAAGGTCGAC+CGG | 0.590424 | 3:-80231005 | None:intergenic |
| TGGGTTGCAGCAGTTGTGGT+GGG | 0.593066 | 3:+80230975 | MsG0380016196.01.T01:CDS |
| GGTTCTCGTTAAACAGGTCA+TGG | 0.598610 | 3:+80230820 | MsG0380016196.01.T01:CDS |
| ACCAGCGTGAACAGAGGCAA+GGG | 0.604969 | 3:-80230907 | None:intergenic |
| CCGTTGCAGCTGGGGCATCG+TGG | 0.605178 | 3:-80230872 | None:intergenic |
| GACCAGCGTGAACAGAGGCA+AGG | 0.610372 | 3:-80230908 | None:intergenic |
| GGCTAGGACGCTTAGACCGG+TGG | 0.613850 | 3:-80230792 | None:intergenic |
| ATTGTGATTCTAGAAGGTAA+AGG | 0.617095 | 3:-80231013 | None:intergenic |
| GTAATCAGCCAATTAGCCAC+CGG | 0.629514 | 3:+80230776 | MsG0380016196.01.T01:CDS |
| GCTAATTGGCTGATTACGAG+TGG | 0.643128 | 3:-80230770 | None:intergenic |
| ACAGAGGCAAGGGACTCGAC+CGG | 0.643316 | 3:-80230897 | None:intergenic |
| CGTCAGACCAGCGTGAACAG+AGG | 0.673770 | 3:-80230913 | None:intergenic |
| TCTAAGCGTCCTAGCCGGAG+CGG | 0.680270 | 3:+80230799 | MsG0380016196.01.T01:CDS |
| GAACCGGTCAACTAAGAAGG+CGG | 0.723769 | 3:-80231048 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTGTGATTCTAGAAGGTAA+AGG | - | Chr3:80231016-80231035 | None:intergenic | 30.0% |
| GGTCGAATTGTGATTCTAGA+AGG | - | Chr3:80231022-80231041 | None:intergenic | 40.0% | |
| ATCGGTTGTAGTACGTGTTC+CGG | + | Chr3:80230941-80230960 | MsG0380016196.01.T01:CDS | 45.0% | |
| ATGGACCAAAAAACCATGGC+CGG | + | Chr3:80230839-80230858 | MsG0380016196.01.T01:CDS | 45.0% | |
| CAACTAAGAAGGCGGTTTGT+CGG | - | Chr3:80231043-80231062 | None:intergenic | 45.0% | |
| GCTAATTGGCTGATTACGAG+TGG | - | Chr3:80230773-80230792 | None:intergenic | 45.0% | |
| GGTCATGGACCAAAAAACCA+TGG | + | Chr3:80230835-80230854 | MsG0380016196.01.T01:CDS | 45.0% | |
| GGTTCTCGTTAAACAGGTCA+TGG | + | Chr3:80230820-80230839 | MsG0380016196.01.T01:CDS | 45.0% | |
| GTAATCAGCCAATTAGCCAC+CGG | + | Chr3:80230776-80230795 | MsG0380016196.01.T01:CDS | 45.0% | |
| TCTAGAAGGTAAAGGTCGAC+CGG | - | Chr3:80231008-80231027 | None:intergenic | 45.0% | |
| TTGTAGTACGTGTTCCGGTT+CGG | + | Chr3:80230946-80230965 | MsG0380016196.01.T01:CDS | 45.0% | |
| CTGTTTAACGAGAACCGCTC+CGG | - | Chr3:80230816-80230835 | None:intergenic | 50.0% | |
| TGTAGTACGTGTTCCGGTTC+GGG | + | Chr3:80230947-80230966 | MsG0380016196.01.T01:CDS | 50.0% | |
| !! | GCATCGTGGAAATGATGGAC+CGG | - | Chr3:80230861-80230880 | None:intergenic | 50.0% |
| !!! | ATGGACCGGCCATGGTTTTT+TGG | - | Chr3:80230847-80230866 | None:intergenic | 50.0% |
| ACCAGCGTGAACAGAGGCAA+GGG | - | Chr3:80230910-80230929 | None:intergenic | 55.0% | |
| AGGTAAAGGTCGACCGGTAC+CGG | - | Chr3:80231002-80231021 | None:intergenic | 55.0% | |
| CGCTTAGACCGGTGGCTAAT+TGG | - | Chr3:80230787-80230806 | None:intergenic | 55.0% | |
| CGGAGCGGTTCTCGTTAAAC+AGG | + | Chr3:80230814-80230833 | MsG0380016196.01.T01:CDS | 55.0% | |
| TGGGTTGCAGCAGTTGTGGT+GGG | + | Chr3:80230975-80230994 | MsG0380016196.01.T01:CDS | 55.0% | |
| ! | ATGGGTTGCAGCAGTTGTGG+TGG | + | Chr3:80230974-80230993 | MsG0380016196.01.T01:CDS | 55.0% |
| ! | CGCTGGTCTGACGGTGATAT+CGG | + | Chr3:80230923-80230942 | MsG0380016196.01.T01:CDS | 55.0% |
| ! | CTCTGTTCACGCTGGTCTGA+CGG | + | Chr3:80230914-80230933 | MsG0380016196.01.T01:CDS | 55.0% |
| !! | CTGGGGCATCGTGGAAATGA+TGG | - | Chr3:80230866-80230885 | None:intergenic | 55.0% |
| !! | TGGAAATGATGGACCGGCCA+TGG | - | Chr3:80230855-80230874 | None:intergenic | 55.0% |
| ACAGAGGCAAGGGACTCGAC+CGG | - | Chr3:80230900-80230919 | None:intergenic | 60.0% | |
| ACCGGTCTAAGCGTCCTAGC+CGG | + | Chr3:80230794-80230813 | MsG0380016196.01.T01:CDS | 60.0% | |
| CGTCAGACCAGCGTGAACAG+AGG | - | Chr3:80230916-80230935 | None:intergenic | 60.0% | |
| GACCAGCGTGAACAGAGGCA+AGG | - | Chr3:80230911-80230930 | None:intergenic | 60.0% | |
| TAACGAGAACCGCTCCGGCT+AGG | - | Chr3:80230811-80230830 | None:intergenic | 60.0% | |
| TCCCTTGCCTCTGTTCACGC+TGG | + | Chr3:80230906-80230925 | MsG0380016196.01.T01:CDS | 60.0% | |
| TCCGGCTAGGACGCTTAGAC+CGG | - | Chr3:80230798-80230817 | None:intergenic | 60.0% | |
| TCTAAGCGTCCTAGCCGGAG+CGG | + | Chr3:80230799-80230818 | MsG0380016196.01.T01:CDS | 60.0% | |
| ! | CGCATGGGTTGCAGCAGTTG+TGG | + | Chr3:80230971-80230990 | MsG0380016196.01.T01:CDS | 60.0% |
| !! | AGTTGTGGTGGGTCCGGTAC+CGG | + | Chr3:80230986-80231005 | MsG0380016196.01.T01:CDS | 60.0% |
| !! | TGCAGCAGTTGTGGTGGGTC+CGG | + | Chr3:80230980-80230999 | MsG0380016196.01.T01:CDS | 60.0% |
| !! | TGTTCCGGTTCGGGTCGCAT+GGG | + | Chr3:80230956-80230975 | MsG0380016196.01.T01:CDS | 60.0% |
| CCACGATGCCCCAGCTGCAA+CGG | + | Chr3:80230872-80230891 | MsG0380016196.01.T01:CDS | 65.0% | |
| GCAACCCATGCGACCCGAAC+CGG | - | Chr3:80230963-80230982 | None:intergenic | 65.0% | |
| GGCTAGGACGCTTAGACCGG+TGG | - | Chr3:80230795-80230814 | None:intergenic | 65.0% | |
| TGCCCCAGCTGCAACGGAAC+CGG | + | Chr3:80230878-80230897 | MsG0380016196.01.T01:CDS | 65.0% | |
| ! | CGACCGGTTCCGTTGCAGCT+GGG | - | Chr3:80230884-80230903 | None:intergenic | 65.0% |
| ! | GACCGGTTCCGTTGCAGCTG+GGG | - | Chr3:80230883-80230902 | None:intergenic | 65.0% |
| ! | TCGACCGGTTCCGTTGCAGC+TGG | - | Chr3:80230885-80230904 | None:intergenic | 65.0% |
| !! | GTGTTCCGGTTCGGGTCGCA+TGG | + | Chr3:80230955-80230974 | MsG0380016196.01.T01:CDS | 65.0% |
| !! | CCGTTGCAGCTGGGGCATCG+TGG | - | Chr3:80230875-80230894 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 80230764 | 80231063 | 80230764 | ID=MsG0380016196.01;Name=MsG0380016196.01 |
| Chr3 | mRNA | 80230764 | 80231063 | 80230764 | ID=MsG0380016196.01.T01;Parent=MsG0380016196.01;Name=MsG0380016196.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|99 |
| Chr3 | exon | 80230764 | 80231063 | 80230764 | ID=MsG0380016196.01.T01:exon:32791;Parent=MsG0380016196.01.T01 |
| Chr3 | CDS | 80230764 | 80231063 | 80230764 | ID=MsG0380016196.01.T01:cds;Parent=MsG0380016196.01.T01 |
| Gene Sequence |
| Protein sequence |
