AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043961.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043961.01.T01 MTR_8g045695 96.154 156 6 0 1 156 1 156 7.03e-111 311
MsG0880043961.01.T01 MTR_8g045735 88.312 154 18 0 1 154 1 154 1.48e-101 288
MsG0880043961.01.T01 MTR_8g045665 87.179 156 20 0 1 156 1 156 7.32e-100 284
MsG0880043961.01.T01 MTR_8g045640 83.333 156 26 0 1 156 1 156 7.28e-96 274
MsG0880043961.01.T01 MTR_8g045560 82.692 156 27 0 1 156 1 156 1.38e-94 270
MsG0880043961.01.T01 MTR_8g045520 78.981 157 32 1 1 156 1 157 1.54e-89 258
MsG0880043961.01.T01 MTR_8g045490 75.159 157 37 2 1 156 1 156 9.84e-86 248
MsG0880043961.01.T01 MTR_8g045400 73.248 157 40 2 1 156 1 156 3.62e-83 241
MsG0880043961.01.T01 MTR_8g045570 71.711 152 42 1 1 151 1 152 9.80e-81 235
MsG0880043961.01.T01 MTR_8g045555 71.053 152 43 1 1 151 1 152 7.36e-80 233
MsG0880043961.01.T01 MTR_8g045300 67.949 156 49 1 1 155 1 156 6.83e-79 231
MsG0880043961.01.T01 MTR_1g030810 43.137 153 83 3 1 150 1 152 1.59e-39 131
MsG0880043961.01.T01 MTR_1g030820 38.562 153 90 3 1 150 1 152 1.25e-37 126
MsG0880043961.01.T01 MTR_1g030840 38.462 156 87 5 1 148 1 155 6.49e-29 104
MsG0880043961.01.T01 MTR_8g012550 34.000 150 95 4 6 153 8 155 2.40e-26 97.8
MsG0880043961.01.T01 MTR_1g031640 38.514 148 81 6 7 151 7 147 1.94e-22 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043961.01.T01 AT5G28010 35.948 153 94 4 3 152 15 166 1.31e-25 96.3
MsG0880043961.01.T01 AT5G28010 35.948 153 94 4 3 152 15 166 1.31e-25 96.3
MsG0880043961.01.T01 AT1G70840 35.294 153 95 4 3 152 20 171 1.01e-24 94.0
MsG0880043961.01.T01 AT1G70830 36.601 153 93 4 3 152 22 173 2.70e-24 92.8
MsG0880043961.01.T01 AT1G70830 36.364 154 94 4 3 153 22 174 3.95e-24 93.2
MsG0880043961.01.T01 AT1G70890 35.948 153 94 4 3 152 7 158 6.26e-24 91.7
MsG0880043961.01.T01 AT1G70830 36.364 154 94 4 3 153 22 174 1.76e-23 92.8
MsG0880043961.01.T01 AT1G70830 36.364 154 94 4 3 153 22 174 4.49e-23 93.2
MsG0880043961.01.T01 AT1G70830 35.294 153 95 4 3 152 184 335 1.57e-21 89.0
MsG0880043961.01.T01 AT5G28000 32.680 153 99 4 3 152 13 164 2.90e-22 87.4
MsG0880043961.01.T01 AT1G70830 35.294 153 95 4 3 152 137 288 5.45e-22 89.7
MsG0880043961.01.T01 AT1G70830 32.990 97 63 2 59 153 31 127 3.65e-11 60.1
MsG0880043961.01.T01 AT1G70850 34.667 150 94 4 3 149 9 157 1.07e-21 87.8
MsG0880043961.01.T01 AT1G70850 34.667 150 94 4 3 149 9 157 2.41e-21 88.2
MsG0880043961.01.T01 AT1G70850 35.664 143 88 4 13 152 175 316 7.55e-21 87.0
MsG0880043961.01.T01 AT1G70850 34.667 150 94 4 3 149 9 157 2.41e-21 88.2
MsG0880043961.01.T01 AT1G70850 35.664 143 88 4 13 152 175 316 7.55e-21 87.0
MsG0880043961.01.T01 AT4G23670 32.680 153 99 4 1 151 1 151 2.05e-20 82.4
MsG0880043961.01.T01 AT1G14930 29.299 157 107 4 1 155 1 155 2.71e-19 79.7
MsG0880043961.01.T01 AT1G70870 34.839 155 79 5 1 150 1 138 7.19e-19 78.2
MsG0880043961.01.T01 AT2G01520 29.412 153 104 4 1 151 1 151 1.10e-18 77.8
MsG0880043961.01.T01 AT2G01530 30.719 153 102 4 1 151 1 151 9.74e-18 75.5
MsG0880043961.01.T01 AT1G70880 29.412 153 104 4 3 152 8 159 3.97e-17 73.9
MsG0880043961.01.T01 AT1G14940 28.662 157 108 4 1 155 1 155 6.06e-17 73.6
MsG0880043961.01.T01 AT1G35260 30.612 147 91 5 9 149 8 149 2.31e-16 72.0
MsG0880043961.01.T01 AT4G14060 28.758 153 105 4 1 151 1 151 2.36e-16 72.0
MsG0880043961.01.T01 AT4G23680 28.947 152 104 4 1 150 1 150 3.01e-16 71.6
MsG0880043961.01.T01 AT1G23130 28.188 149 104 3 3 149 10 157 4.83e-16 71.2
MsG0880043961.01.T01 AT1G24020 30.968 155 98 4 1 150 1 151 5.03e-16 71.2
MsG0880043961.01.T01 AT1G24020 30.968 155 98 4 1 150 1 151 5.03e-16 71.2
MsG0880043961.01.T01 AT1G14950 28.025 157 109 4 1 155 1 155 8.11e-16 70.5
MsG0880043961.01.T01 AT1G23120 28.667 150 99 5 3 149 1 145 1.76e-15 69.3
MsG0880043961.01.T01 AT1G23120 27.660 141 96 4 10 149 60 195 4.61e-15 69.7
MsG0880043961.01.T01 AT1G30990 27.815 151 104 5 1 149 1 148 1.56e-13 64.3
MsG0880043961.01.T01 AT1G14960 26.452 155 110 4 1 153 1 153 8.69e-13 62.4
MsG0880043961.01.T01 AT1G35310 27.338 139 97 4 13 149 12 148 1.60e-12 62.0
MsG0880043961.01.T01 AT3G26460 26.797 153 109 3 1 151 1 152 2.27e-12 61.6
MsG0880043961.01.T01 AT3G26450 24.675 154 111 5 1 151 1 152 4.26e-11 58.2

Find 27 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATCACATACAAGCTATT+TGG 0.273919 8:-33443022 MsG0880043961.01.T01:CDS
TGGAGATATTGAAAACTATA+AGG 0.391039 8:-33442999 MsG0880043961.01.T01:CDS
ATGGAAATTCTGCTGTTAGA+TGG 0.411619 8:-33442940 MsG0880043961.01.T01:CDS
GGTAAACTTATTACTGAACT+TGG 0.415223 8:-33444201 MsG0880043961.01.T01:CDS
AGGTAGTCCATCCAGCCATT+TGG 0.456227 8:+33442878 None:intergenic
AAACACTGGACTTATGTCAT+AGG 0.470664 8:-33444048 MsG0880043961.01.T01:intron
AAAAGCACTAGAGATATTGA+TGG 0.477335 8:-33442851 MsG0880043961.01.T01:CDS
AGCTTCATGAGGGTGAAGAT+TGG 0.493681 8:-33444089 MsG0880043961.01.T01:CDS
TCATCTTGTCAAAGGGGAAA+AGG 0.504175 8:-33442828 MsG0880043961.01.T01:CDS
AGATTGGCATCATACTGATT+CGG 0.504369 8:-33444073 MsG0880043961.01.T01:CDS
TTGATGGTCATCTTGTCAAA+GGG 0.506740 8:-33442835 MsG0880043961.01.T01:CDS
ATCTTCACCCTCATGAAGCT+TGG 0.519812 8:+33444092 None:intergenic
ATCACATACAAGCTATTTGG+TGG 0.530005 8:-33443019 MsG0880043961.01.T01:CDS
GTAAACTTATTACTGAACTT+GGG 0.542207 8:-33444200 MsG0880043961.01.T01:CDS
ATTGATGGTCATCTTGTCAA+AGG 0.543582 8:-33442836 MsG0880043961.01.T01:CDS
AGTTTGTAGAACTTGTCAGC+TGG 0.569465 8:+33444168 None:intergenic
GAAGTGCTTAACAAGGCTGA+TGG 0.573383 8:-33442959 MsG0880043961.01.T01:CDS
ATGTTGATACTCCAAATGGC+TGG 0.578150 8:-33442889 MsG0880043961.01.T01:CDS
TGATACTCCAAATGGCTGGA+TGG 0.587093 8:-33442885 MsG0880043961.01.T01:CDS
GAGGATGTTGATACTCCAAA+TGG 0.593330 8:-33442893 MsG0880043961.01.T01:CDS
ATACTGATTCGGTTAAACAC+TGG 0.606046 8:-33444062 MsG0880043961.01.T01:CDS
TCATCATACCAAGCTTCATG+AGG 0.610699 8:-33444100 MsG0880043961.01.T01:CDS
CATCATACCAAGCTTCATGA+GGG 0.642119 8:-33444099 MsG0880043961.01.T01:CDS
CATCTTAGAAGTGCTTAACA+AGG 0.644958 8:-33442966 MsG0880043961.01.T01:CDS
TGATGGTCATCTTGTCAAAG+GGG 0.652198 8:-33442834 MsG0880043961.01.T01:CDS
TGACATAACAACAGATGGCG+AGG 0.663434 8:-33443092 MsG0880043961.01.T01:intron
TGATTATGAGAAGAACAATG+AGG 0.694185 8:-33442912 MsG0880043961.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTACTATAAAATAGTA+AGG - Chr8:33443708-33443727 MsG0880043961.01.T01:intron 10.0%
!! AATTAATAAAAAAAAGGTAA+CGG + Chr8:33443374-33443393 None:intergenic 10.0%
!! TTATCTTATTATAATATTTC+TGG - Chr8:33443846-33443865 MsG0880043961.01.T01:intron 10.0%
!!! AAAACTTATTTTATTCTTTT+TGG - Chr8:33443902-33443921 MsG0880043961.01.T01:intron 10.0%
!! AAATTCTAAATTAAGTCAAT+AGG + Chr8:33443206-33443225 None:intergenic 15.0%
!! AGATAATCTATATAAAAAGT+AGG - Chr8:33443576-33443595 MsG0880043961.01.T01:intron 15.0%
!! GATAATCTATATAAAAAGTA+GGG - Chr8:33443577-33443596 MsG0880043961.01.T01:intron 15.0%
!! TATAGTAATAAAAAGAAGAT+TGG - Chr8:33443092-33443111 MsG0880043961.01.T01:intron 15.0%
!!! GAATAAAATAAGTTTTTCAA+AGG + Chr8:33443898-33443917 None:intergenic 15.0%
!!! TTATTTTACTACAAACTTTA+AGG + Chr8:33443643-33443662 None:intergenic 15.0%
!! CATCATATAAGATCTTTAAA+AGG - Chr8:33443749-33443768 MsG0880043961.01.T01:intron 20.0%
!! TATTATTTAGAAAAAGGAAG+AGG - Chr8:33443426-33443445 MsG0880043961.01.T01:intron 20.0%
! AAAAAATTATACTCGTACCA+TGG + Chr8:33443517-33443536 None:intergenic 25.0%
! AAAATCACATACAAGCTATT+TGG - Chr8:33444008-33444027 MsG0880043961.01.T01:intron 25.0%
! AGTAATAAAAAGAAGATTGG+TGG - Chr8:33443095-33443114 MsG0880043961.01.T01:intron 25.0%
! GTAAACTTATTACTGAACTT+GGG - Chr8:33442830-33442849 MsG0880043961.01.T01:CDS 25.0%
! GTAATAAAAAGAAGATTGGT+GGG - Chr8:33443096-33443115 MsG0880043961.01.T01:intron 25.0%
! TATCAAACAAGATGATGATT+TGG + Chr8:33443034-33443053 None:intergenic 25.0%
! TCTTCGTTATAGAATTCATT+TGG + Chr8:33443132-33443151 None:intergenic 25.0%
! TGGAGATATTGAAAACTATA+AGG - Chr8:33444031-33444050 MsG0880043961.01.T01:intron 25.0%
!!! ATCTTTAAAAGGTGTTTTAG+AGG - Chr8:33443760-33443779 MsG0880043961.01.T01:intron 25.0%
GGTAAACTTATTACTGAACT+TGG - Chr8:33442829-33442848 MsG0880043961.01.T01:CDS 30.0%
GTTTGTATGATATGATCCTT+CGG - Chr8:33443599-33443618 MsG0880043961.01.T01:intron 30.0%
TGATTATGAGAAGAACAATG+AGG - Chr8:33444118-33444137 MsG0880043961.01.T01:CDS 30.0%
!! AAAAGCACTAGAGATATTGA+TGG - Chr8:33444179-33444198 MsG0880043961.01.T01:CDS 30.0%
AAACACTGGACTTATGTCAT+AGG - Chr8:33442982-33443001 MsG0880043961.01.T01:CDS 35.0%
AAAGCAACAAATGACCCTAA+AGG - Chr8:33443459-33443478 MsG0880043961.01.T01:intron 35.0%
AGTAGCATCTCTTTCCTTTA+GGG + Chr8:33443476-33443495 None:intergenic 35.0%
ATCACATACAAGCTATTTGG+TGG - Chr8:33444011-33444030 MsG0880043961.01.T01:intron 35.0%
ATGGAAATTCTGCTGTTAGA+TGG - Chr8:33444090-33444109 MsG0880043961.01.T01:CDS 35.0%
TATCGAAATCATTGTACACG+AGG - Chr8:33443309-33443328 MsG0880043961.01.T01:intron 35.0%
! AGATTGGCATCATACTGATT+CGG - Chr8:33442957-33442976 MsG0880043961.01.T01:CDS 35.0%
! ATTGATGGTCATCTTGTCAA+AGG - Chr8:33444194-33444213 MsG0880043961.01.T01:CDS 35.0%
! TATCTCTAGTGCTTTTGCTA+AGG + Chr8:33444175-33444194 None:intergenic 35.0%
! TTGATGGTCATCTTGTCAAA+GGG - Chr8:33444195-33444214 MsG0880043961.01.T01:CDS 35.0%
!! ATACTGATTCGGTTAAACAC+TGG - Chr8:33442968-33442987 MsG0880043961.01.T01:CDS 35.0%
!! CATCTTAGAAGTGCTTAACA+AGG - Chr8:33444064-33444083 MsG0880043961.01.T01:CDS 35.0%
AACCACCTTTGTATTCACCA+TGG - Chr8:33443497-33443516 MsG0880043961.01.T01:intron 40.0%
AGTTTGTAGAACTTGTCAGC+TGG + Chr8:33442865-33442884 None:intergenic 40.0%
ATGTTGATACTCCAAATGGC+TGG - Chr8:33444141-33444160 MsG0880043961.01.T01:CDS 40.0%
CATCATACCAAGCTTCATGA+GGG - Chr8:33442931-33442950 MsG0880043961.01.T01:CDS 40.0%
GAGGATGTTGATACTCCAAA+TGG - Chr8:33444137-33444156 MsG0880043961.01.T01:CDS 40.0%
GAGTAGCATCTCTTTCCTTT+AGG + Chr8:33443477-33443496 None:intergenic 40.0%
TACCATGGTGAATACAAAGG+TGG + Chr8:33443502-33443521 None:intergenic 40.0%
TCATCATACCAAGCTTCATG+AGG - Chr8:33442930-33442949 MsG0880043961.01.T01:CDS 40.0%
TCATCTTGTCAAAGGGGAAA+AGG - Chr8:33444202-33444221 MsG0880043961.01.T01:CDS 40.0%
TCGTACCATGGTGAATACAA+AGG + Chr8:33443505-33443524 None:intergenic 40.0%
TGAGACTGATCTAACAACGA+AGG - Chr8:33443169-33443188 MsG0880043961.01.T01:intron 40.0%
TGATGGTCATCTTGTCAAAG+GGG - Chr8:33444196-33444215 MsG0880043961.01.T01:CDS 40.0%
! CGTCTTTAGACATTTTCCGA+AGG + Chr8:33443618-33443637 None:intergenic 40.0%
AGCTTCATGAGGGTGAAGAT+TGG - Chr8:33442941-33442960 MsG0880043961.01.T01:CDS 45.0%
ATCTTCACCCTCATGAAGCT+TGG + Chr8:33442941-33442960 None:intergenic 45.0%
GCCATCTGTTGTTATGTCAG+AGG + Chr8:33443937-33443956 None:intergenic 45.0%
GCCTCTGACATAACAACAGA+TGG - Chr8:33443933-33443952 MsG0880043961.01.T01:intron 45.0%
TGACATAACAACAGATGGCG+AGG - Chr8:33443938-33443957 MsG0880043961.01.T01:intron 45.0%
TGATACTCCAAATGGCTGGA+TGG - Chr8:33444145-33444164 MsG0880043961.01.T01:CDS 45.0%
TTGTACACGAGGTGAAGTAC+TGG - Chr8:33443320-33443339 MsG0880043961.01.T01:intron 45.0%
! GAAGTGCTTAACAAGGCTGA+TGG - Chr8:33444071-33444090 MsG0880043961.01.T01:CDS 45.0%
!! AAATAATATATATTATGAAT+GGG + Chr8:33443414-33443433 None:intergenic 5.0%
!! AATATATATTATTTAGAAAA+AGG - Chr8:33443420-33443439 MsG0880043961.01.T01:intron 5.0%
!! ATTCTTAATTAATAAAAAAA+AGG + Chr8:33443380-33443399 None:intergenic 5.0%
!! TAAATAATATATATTATGAA+TGG + Chr8:33443415-33443434 None:intergenic 5.0%
!!! TAATTTAGAATTTTTTTAAT+TGG - Chr8:33443213-33443232 MsG0880043961.01.T01:intron 5.0%
AGGTAGTCCATCCAGCCATT+TGG + Chr8:33444155-33444174 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr8 gene 33442817 33444235 33442817 ID=MsG0880043961.01;Name=MsG0880043961.01
Chr8 mRNA 33442817 33444235 33442817 ID=MsG0880043961.01.T01;Parent=MsG0880043961.01;Name=MsG0880043961.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|1|2|0|156
Chr8 exon 33444049 33444235 33444049 ID=MsG0880043961.01.T01:exon:10193;Parent=MsG0880043961.01.T01
Chr8 exon 33442817 33443100 33442817 ID=MsG0880043961.01.T01:exon:10192;Parent=MsG0880043961.01.T01
Chr8 CDS 33444049 33444235 33444049 ID=MsG0880043961.01.T01:cds;Parent=MsG0880043961.01.T01
Chr8 CDS 33442817 33443100 33442817 ID=MsG0880043961.01.T01:cds;Parent=MsG0880043961.01.T01
Gene Sequence

>MsG0880043961.01.T01

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATAAAAACACCAGCTGACAAGTTCTACAAACTATTTGCATCAGAACTTCACGAAGTGCAAAATGTTTGTGAAAGAGTTCATCATACCAAGCTTCATGAGGGTGAAGATTGGCATCATACTGATTCGGTTAAACACTGGACTTATGTCATAGATGGCGAGGTACACACATGCAATGAGAGTGTTGAAGAAGTTGATGATCAGAACAAAAAAATCACATACAAGCTATTTGGTGGAGATATTGAAAACTATAAGGTATTTAAGCTCATCTTAGAAGTGCTTAACAAGGCTGATGGAAATTCTGCTGTTAGATGGACTATTGATTATGAGAAGAACAATGAGGATGTTGATACTCCAAATGGCTGGATGGACTACCTTAGCAAAAGCACTAGAGATATTGATGGTCATCTTGTCAAAGGGGAAAAGGTAGCTCTATAA

Protein sequence

>MsG0880043961.01.T01

MVLAGKLITELGIKTPADKFYKLFASELHEVQNVCERVHHTKLHEGEDWHHTDSVKHWTYVIDGEVHTCNESVEEVDDQNKKITYKLFGGDIENYKVFKLILEVLNKADGNSAVRWTIDYEKNNEDVDTPNGWMDYLSKSTRDIDGHLVKGEKVAL*