Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004559.01.T01 | XP_003591068.2 | 93.51 | 339 | 14 | 4 | 1 | 333 | 1 | 337 | 0 | 613 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004559.01.T01 | Q9C7T4 | 46.491 | 342 | 133 | 9 | 14 | 330 | 1 | 317 | 3.15E-81 | 252 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004559.01.T01 | G7I7I3 | 93.510 | 339 | 14 | 4 | 1 | 333 | 1 | 337 | 0.0 | 613 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048633.01 | MsG0180004559.01 | 0.834385 | 3.089696e-56 | 3.660913e-53 |
| MsG0080048652.01 | MsG0180004559.01 | 0.849492 | 3.123973e-60 | 5.932330e-57 |
| MsG0080048694.01 | MsG0180004559.01 | -0.821913 | 3.135441e-53 | 2.585369e-50 |
| MsG0080048695.01 | MsG0180004559.01 | -0.821201 | 4.579717e-53 | 3.701602e-50 |
| MsG0080048889.01 | MsG0180004559.01 | 0.800201 | 1.610258e-48 | 7.452751e-46 |
| MsG0080048948.01 | MsG0180004559.01 | -0.850420 | 1.717460e-60 | 3.362487e-57 |
| MsG0180000076.01 | MsG0180004559.01 | 0.841037 | 6.057756e-58 | 8.790188e-55 |
| MsG0180001925.01 | MsG0180004559.01 | -0.832727 | 8.015180e-56 | 9.036579e-53 |
| MsG0180002808.01 | MsG0180004559.01 | 0.819692 | 1.016563e-52 | 7.878137e-50 |
| MsG0180003430.01 | MsG0180004559.01 | 0.803579 | 3.259465e-49 | 1.643992e-46 |
| MsG0180004027.01 | MsG0180004559.01 | 0.804549 | 2.048314e-49 | 1.059114e-46 |
| MsG0180004124.01 | MsG0180004559.01 | -0.844191 | 8.809619e-59 | 1.411397e-55 |
| MsG0180004125.01 | MsG0180004559.01 | -0.843415 | 1.421372e-58 | 2.222404e-55 |
| MsG0180004510.01 | MsG0180004559.01 | 0.807145 | 5.831746e-50 | 3.223468e-47 |
| MsG0180004559.01 | MsG0180004737.01 | -0.804075 | 2.570826e-49 | 1.313271e-46 |
| MsG0180004559.01 | MsG0180005369.01 | -0.835126 | 2.011313e-56 | 2.436425e-53 |
| MsG0180004559.01 | MsG0180006026.01 | 0.805042 | 1.615885e-49 | 8.462268e-47 |
| MsG0180004559.01 | MsG0180006182.01 | 0.820448 | 6.821194e-53 | 5.398964e-50 |
| MsG0180004559.01 | MsG0180006191.01 | 0.804955 | 1.684486e-49 | 8.802460e-47 |
| MsG0180004559.01 | MsG0280006884.01 | 0.829007 | 6.547306e-55 | 6.616137e-52 |
| MsG0180004559.01 | MsG0280007419.01 | 0.804381 | 2.219740e-49 | 1.142858e-46 |
| MsG0180004559.01 | MsG0280007653.01 | -0.811148 | 8.094156e-51 | 4.972074e-48 |
| MsG0180004559.01 | MsG0280007786.01 | 0.843472 | 1.371903e-58 | 2.148757e-55 |
| MsG0180004559.01 | MsG0280007844.01 | 0.832314 | 1.014408e-55 | 1.129518e-52 |
| MsG0180004559.01 | MsG0280008407.01 | 0.850889 | 1.268147e-60 | 2.521000e-57 |
| MsG0180004559.01 | MsG0280008820.01 | -0.816634 | 4.998599e-52 | 3.560897e-49 |
| MsG0180004559.01 | MsG0280009241.01 | 0.803392 | 3.563508e-49 | 1.788611e-46 |
| MsG0180004559.01 | MsG0280010142.01 | -0.812269 | 4.616634e-51 | 2.922082e-48 |
| MsG0180004559.01 | MsG0280010303.01 | 0.801036 | 1.088128e-48 | 5.144422e-46 |
| MsG0180004559.01 | MsG0380013041.01 | 0.817618 | 3.003459e-52 | 2.198051e-49 |
| MsG0180004559.01 | MsG0380013063.01 | -0.833818 | 4.285808e-56 | 4.992495e-53 |
| MsG0180004559.01 | MsG0380013415.01 | 0.800199 | 1.611794e-48 | 7.459406e-46 |
| MsG0180004559.01 | MsG0380013892.01 | -0.805628 | 1.217676e-49 | 6.473785e-47 |
| MsG0180004559.01 | MsG0380013928.01 | -0.813751 | 2.184575e-51 | 1.438728e-48 |
| MsG0180004559.01 | MsG0380013986.01 | -0.814134 | 1.798654e-51 | 1.196963e-48 |
| MsG0180004559.01 | MsG0380013997.01 | -0.813936 | 1.988789e-51 | 1.316368e-48 |
| MsG0180004559.01 | MsG0380014016.01 | -0.813416 | 2.587818e-51 | 1.688980e-48 |
| MsG0180004559.01 | MsG0380014029.01 | -0.816195 | 6.267761e-52 | 4.411896e-49 |
| MsG0180004559.01 | MsG0380014735.01 | -0.809909 | 1.498557e-50 | 8.908977e-48 |
| MsG0180004559.01 | MsG0380014754.01 | 0.833717 | 4.541889e-56 | 5.274137e-53 |
| MsG0180004559.01 | MsG0380014881.01 | 0.813011 | 3.176393e-51 | 2.050392e-48 |
| MsG0180004559.01 | MsG0380015802.01 | -0.803715 | 3.053931e-49 | 1.545598e-46 |
| MsG0180004559.01 | MsG0380015828.01 | -0.813675 | 2.270309e-51 | 1.492188e-48 |
| MsG0180004559.01 | MsG0380016065.01 | -0.827765 | 1.306020e-54 | 1.272835e-51 |
| MsG0180004559.01 | MsG0380016196.01 | -0.801078 | 1.066768e-48 | 5.048704e-46 |
| MsG0180004559.01 | MsG0380017231.01 | 0.888839 | 4.132936e-73 | 3.253681e-69 |
| MsG0180004559.01 | MsG0380017730.01 | 0.844190 | 8.814236e-59 | 1.412093e-55 |
| MsG0180004559.01 | MsG0380017997.01 | 0.813621 | 2.333593e-51 | 1.531518e-48 |
| MsG0180004559.01 | MsG0480019028.01 | -0.811559 | 6.588775e-51 | 4.091600e-48 |
| MsG0180004559.01 | MsG0480019247.01 | -0.808615 | 2.839244e-50 | 1.630828e-47 |
| MsG0180004559.01 | MsG0480019588.01 | 0.818303 | 2.102787e-52 | 1.568157e-49 |
| MsG0180004559.01 | MsG0480020263.01 | 0.807542 | 4.804230e-50 | 2.683070e-47 |
| MsG0180004559.01 | MsG0480020537.01 | 0.800199 | 1.612184e-48 | 7.461150e-46 |
| MsG0180004559.01 | MsG0480021175.01 | 0.811873 | 5.629890e-51 | 3.525189e-48 |
| MsG0180004559.01 | MsG0480021972.01 | 0.828882 | 7.022461e-55 | 7.069983e-52 |
| MsG0180004559.01 | MsG0480022167.01 | 0.806642 | 7.450905e-50 | 4.065002e-47 |
| MsG0180004559.01 | MsG0480023195.01 | 0.857107 | 2.044596e-62 | 4.990155e-59 |
| MsG0180004559.01 | MsG0480023322.01 | 0.810611 | 1.057627e-50 | 6.404672e-48 |
| MsG0180004559.01 | MsG0480023324.01 | 0.876082 | 1.852360e-68 | 8.860719e-65 |
| MsG0180004559.01 | MsG0480023532.01 | 0.805005 | 1.645013e-49 | 8.606611e-47 |
| MsG0180004559.01 | MsG0580024119.01 | 0.846950 | 1.573650e-59 | 2.753687e-56 |
| MsG0180004559.01 | MsG0580024478.01 | 0.820519 | 6.571691e-53 | 5.211294e-50 |
| MsG0180004559.01 | MsG0580025385.01 | 0.833792 | 4.351776e-56 | 5.065270e-53 |
| MsG0180004559.01 | MsG0580025443.01 | 0.865163 | 7.208778e-65 | 2.322652e-61 |
| MsG0180004559.01 | MsG0580025444.01 | 0.854984 | 8.549722e-62 | 1.944328e-58 |
| MsG0180004559.01 | MsG0580025463.01 | 0.800286 | 1.547703e-48 | 7.178950e-46 |
| MsG0180004559.01 | MsG0580025464.01 | 0.825706 | 4.049753e-54 | 3.719672e-51 |
| MsG0180004559.01 | MsG0580025486.01 | -0.813632 | 2.320050e-51 | 1.523094e-48 |
| MsG0180004559.01 | MsG0580025575.01 | 0.814459 | 1.524576e-51 | 1.023558e-48 |
| MsG0180004559.01 | MsG0580025620.01 | 0.840187 | 1.011296e-57 | 1.429408e-54 |
| MsG0180004559.01 | MsG0580025822.01 | 0.824917 | 6.222752e-54 | 5.588918e-51 |
| MsG0180004559.01 | MsG0580028330.01 | 0.859230 | 4.776602e-63 | 1.252470e-59 |
| MsG0180004559.01 | MsG0580028565.01 | 0.808292 | 3.326959e-50 | 1.895040e-47 |
| MsG0180004559.01 | MsG0580029023.01 | 0.806896 | 6.582288e-50 | 3.614947e-47 |
| MsG0180004559.01 | MsG0580029623.01 | 0.849097 | 4.023881e-60 | 7.544998e-57 |
| MsG0180004559.01 | MsG0580029792.01 | -0.816736 | 4.741966e-52 | 3.387494e-49 |
| MsG0180004559.01 | MsG0580030179.01 | 0.841073 | 5.927801e-58 | 8.610215e-55 |
| MsG0180004559.01 | MsG0680030658.01 | -0.801686 | 8.010180e-49 | 3.849257e-46 |
| MsG0180004559.01 | MsG0680030762.01 | 0.803563 | 3.284503e-49 | 1.655954e-46 |
| MsG0180004559.01 | MsG0680031178.01 | 0.821922 | 3.120971e-53 | 2.574062e-50 |
| MsG0180004559.01 | MsG0680031686.01 | 0.801880 | 7.307179e-49 | 3.529095e-46 |
| MsG0180004559.01 | MsG0680034415.01 | 0.829648 | 4.577144e-55 | 4.713599e-52 |
| MsG0180004559.01 | MsG0680034477.01 | 0.807678 | 4.495113e-50 | 2.519334e-47 |
| MsG0180004559.01 | MsG0680034733.01 | 0.804235 | 2.381222e-49 | 1.221307e-46 |
| MsG0180004559.01 | MsG0680035870.01 | -0.822246 | 2.625090e-53 | 2.185167e-50 |
| MsG0180004559.01 | MsG0780036890.01 | 0.813967 | 1.957707e-51 | 1.296939e-48 |
| MsG0180004559.01 | MsG0780037982.01 | -0.823730 | 1.183338e-53 | 1.027502e-50 |
| MsG0180004559.01 | MsG0780038810.01 | 0.859967 | 2.866373e-63 | 7.709446e-60 |
| MsG0180004559.01 | MsG0780038823.01 | 0.824660 | 7.154749e-54 | 6.378993e-51 |
| MsG0180004559.01 | MsG0780039303.01 | 0.819200 | 1.315641e-52 | 1.005728e-49 |
| MsG0180004559.01 | MsG0780039642.01 | 0.813900 | 2.025022e-51 | 1.339117e-48 |
| MsG0180004559.01 | MsG0780039914.01 | 0.800337 | 1.511376e-48 | 7.019431e-46 |
| MsG0180004559.01 | MsG0780039916.01 | 0.832276 | 1.036781e-55 | 1.153136e-52 |
| MsG0180004559.01 | MsG0780040266.01 | 0.862239 | 5.834198e-64 | 1.696841e-60 |
| MsG0180004559.01 | MsG0780040799.01 | 0.804069 | 2.578248e-49 | 1.316885e-46 |
| MsG0180004559.01 | MsG0780041100.01 | 0.829194 | 5.899129e-55 | 5.995132e-52 |
| MsG0180004559.01 | MsG0780041118.01 | 0.807359 | 5.252409e-50 | 2.919476e-47 |
| MsG0180004559.01 | MsG0780041363.01 | 0.809073 | 2.265530e-50 | 1.317275e-47 |
| MsG0180004559.01 | MsG0780041531.01 | -0.826097 | 3.269590e-54 | 3.036640e-51 |
| MsG0180004559.01 | MsG0780041591.01 | 0.819863 | 9.290274e-53 | 7.234483e-50 |
| MsG0180004559.01 | MsG0880041837.01 | 0.801078 | 1.066690e-48 | 5.048352e-46 |
| MsG0180004559.01 | MsG0880043261.01 | 0.850622 | 1.507591e-60 | 2.970876e-57 |
| MsG0180004559.01 | MsG0880043948.01 | 0.828297 | 9.720135e-55 | 9.621524e-52 |
| MsG0180004559.01 | MsG0880043961.01 | 0.819620 | 1.055554e-52 | 8.163737e-50 |
| MsG0180004559.01 | MsG0880043983.01 | 0.869543 | 2.861596e-66 | 1.078084e-62 |
| MsG0180004559.01 | MsG0880043989.01 | 0.809293 | 2.032802e-50 | 1.188935e-47 |
| MsG0180004559.01 | MsG0880044688.01 | 0.838929 | 2.147839e-57 | 2.920115e-54 |
| MsG0180004559.01 | MsG0880044989.01 | 0.817272 | 3.593301e-52 | 2.604852e-49 |
| MsG0180004559.01 | MsG0880045482.01 | 0.812875 | 3.401034e-51 | 2.187402e-48 |
| MsG0180004559.01 | MsG0880045503.01 | 0.808264 | 3.373574e-50 | 1.920221e-47 |
| MsG0180004559.01 | MsG0880045703.01 | -0.801656 | 8.122027e-49 | 3.900116e-46 |
| MsG0180004559.01 | MsG0880045989.01 | 0.802963 | 4.370938e-49 | 2.169923e-46 |
| MsG0180004559.01 | MsG0880046174.01 | -0.822781 | 1.971736e-53 | 1.666553e-50 |
| MsG0180004559.01 | MsG0880046630.01 | -0.809937 | 1.478034e-50 | 8.793380e-48 |
| MsG0180004559.01 | MsG0880046693.01 | 0.817370 | 3.415341e-52 | 2.482670e-49 |
| MsG0180004559.01 | MsG0880047465.01 | 0.807785 | 4.266200e-50 | 2.397738e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004559.01.T01 | MTR_1g082480 | 93.510 | 339 | 14 | 4 | 1 | 333 | 1 | 337 | 0.0 | 613 |
| MsG0180004559.01.T01 | MTR_1g082480 | 93.528 | 309 | 12 | 4 | 1 | 303 | 1 | 307 | 0.0 | 554 |
| MsG0180004559.01.T01 | MTR_7g113830 | 56.897 | 348 | 100 | 11 | 1 | 333 | 1 | 313 | 5.96e-123 | 356 |
| MsG0180004559.01.T01 | MTR_3g099620 | 50.000 | 330 | 136 | 7 | 14 | 329 | 1 | 315 | 6.18e-100 | 297 |
| MsG0180004559.01.T01 | MTR_5g064950 | 43.614 | 321 | 152 | 7 | 14 | 330 | 1 | 296 | 5.49e-80 | 246 |
| MsG0180004559.01.T01 | MTR_8g066770 | 53.774 | 212 | 87 | 2 | 126 | 332 | 104 | 309 | 2.03e-74 | 232 |
| MsG0180004559.01.T01 | MTR_5g064950 | 43.197 | 294 | 138 | 7 | 14 | 303 | 1 | 269 | 8.17e-70 | 220 |
| MsG0180004559.01.T01 | MTR_8g024210 | 51.244 | 201 | 88 | 2 | 126 | 326 | 171 | 361 | 4.62e-58 | 191 |
| MsG0180004559.01.T01 | MTR_0194s0050 | 47.748 | 222 | 97 | 5 | 118 | 326 | 166 | 381 | 8.00e-58 | 191 |
| MsG0180004559.01.T01 | MTR_1g028470 | 33.218 | 289 | 144 | 8 | 54 | 321 | 30 | 290 | 6.14e-36 | 132 |
| MsG0180004559.01.T01 | MTR_2g022280 | 40.860 | 186 | 107 | 2 | 136 | 321 | 1 | 183 | 6.54e-35 | 126 |
| MsG0180004559.01.T01 | MTR_3g090850 | 33.333 | 252 | 120 | 6 | 117 | 321 | 76 | 326 | 1.58e-33 | 126 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004559.01.T01 | AT1G72210 | 46.491 | 342 | 133 | 9 | 14 | 330 | 1 | 317 | 3.21e-82 | 252 |
| MsG0180004559.01.T01 | AT1G22490 | 44.248 | 339 | 132 | 11 | 14 | 330 | 1 | 304 | 2.75e-79 | 244 |
| MsG0180004559.01.T01 | AT1G72210 | 46.984 | 315 | 117 | 10 | 14 | 303 | 1 | 290 | 1.21e-72 | 228 |
| MsG0180004559.01.T01 | AT1G22490 | 43.910 | 312 | 118 | 11 | 14 | 303 | 1 | 277 | 8.13e-68 | 216 |
| MsG0180004559.01.T01 | AT3G24140 | 43.556 | 225 | 112 | 3 | 115 | 327 | 176 | 397 | 8.28e-54 | 181 |
| MsG0180004559.01.T01 | AT3G24140 | 43.556 | 225 | 112 | 3 | 115 | 327 | 176 | 397 | 1.08e-53 | 181 |
| MsG0180004559.01.T01 | AT4G01460 | 43.602 | 211 | 107 | 2 | 126 | 331 | 105 | 308 | 4.70e-53 | 177 |
| MsG0180004559.01.T01 | AT4G01460 | 43.602 | 211 | 107 | 2 | 126 | 331 | 105 | 308 | 4.70e-53 | 177 |
| MsG0180004559.01.T01 | AT5G46690 | 45.455 | 231 | 93 | 4 | 126 | 329 | 78 | 302 | 1.09e-51 | 174 |
| MsG0180004559.01.T01 | AT5G65320 | 45.024 | 211 | 106 | 4 | 124 | 329 | 90 | 295 | 6.10e-50 | 168 |
| MsG0180004559.01.T01 | AT3G61950 | 45.455 | 198 | 94 | 3 | 126 | 322 | 117 | 301 | 1.13e-47 | 163 |
| MsG0180004559.01.T01 | AT2G46810 | 46.701 | 197 | 92 | 4 | 127 | 322 | 112 | 296 | 1.58e-47 | 162 |
| MsG0180004559.01.T01 | AT3G61950 | 45.000 | 200 | 96 | 3 | 126 | 324 | 168 | 354 | 3.14e-47 | 163 |
| MsG0180004559.01.T01 | AT2G46810 | 46.535 | 202 | 89 | 5 | 127 | 324 | 184 | 370 | 6.97e-47 | 162 |
| MsG0180004559.01.T01 | AT2G46810 | 46.535 | 202 | 89 | 5 | 127 | 324 | 172 | 358 | 7.04e-47 | 162 |
| MsG0180004559.01.T01 | AT5G65320 | 46.196 | 184 | 90 | 3 | 124 | 303 | 90 | 268 | 6.50e-43 | 149 |
| MsG0180004559.01.T01 | AT2G46810 | 41.304 | 230 | 88 | 6 | 127 | 324 | 112 | 326 | 6.99e-42 | 148 |
| MsG0180004559.01.T01 | AT3G61950 | 46.667 | 165 | 75 | 2 | 126 | 290 | 117 | 268 | 1.71e-41 | 146 |
| MsG0180004559.01.T01 | AT3G61950 | 46.667 | 165 | 75 | 2 | 126 | 290 | 168 | 319 | 5.97e-41 | 146 |
| MsG0180004559.01.T01 | AT5G46690 | 44.390 | 205 | 81 | 4 | 126 | 303 | 78 | 276 | 2.99e-40 | 143 |
| MsG0180004559.01.T01 | AT3G06120 | 37.234 | 188 | 95 | 2 | 136 | 309 | 1 | 179 | 2.43e-32 | 120 |
| MsG0180004559.01.T01 | AT5G53210 | 56.923 | 65 | 28 | 0 | 134 | 198 | 100 | 164 | 5.86e-20 | 89.7 |
| MsG0180004559.01.T01 | AT5G53210 | 56.923 | 65 | 28 | 0 | 134 | 198 | 100 | 164 | 5.89e-20 | 89.7 |
| MsG0180004559.01.T01 | AT3G61950 | 71.429 | 42 | 12 | 0 | 126 | 167 | 168 | 209 | 4.46e-13 | 67.8 |
| MsG0180004559.01.T01 | AT3G61950 | 71.429 | 42 | 12 | 0 | 126 | 167 | 168 | 209 | 4.46e-13 | 67.8 |
Find 43 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGATTCCATAACTAAA+AGG | 0.292800 | 1:+79542800 | None:intergenic |
| GGTGATCAAGCTTCTATAAT+TGG | 0.293785 | 1:-79542091 | MsG0180004559.01.T01:CDS |
| ACTCTATCTCTTTCCTCTTC+AGG | 0.299868 | 1:+79542869 | None:intergenic |
| CAGCTCTTGAAAATGGTATC+TGG | 0.309667 | 1:-79541791 | MsG0180004559.01.T01:CDS |
| AATGAAAAGAATTCAGAAAA+TGG | 0.318161 | 1:+79541962 | None:intergenic |
| GCCAAAACAGCTCTTGAAAA+TGG | 0.324313 | 1:-79541798 | MsG0180004559.01.T01:CDS |
| TCTAATTCTGCAGCTACTAT+TGG | 0.326308 | 1:-79541914 | MsG0180004559.01.T01:CDS |
| GTTGAAGATGATTGCAAGTT+GGG | 0.350399 | 1:-79541577 | MsG0180004559.01.T01:CDS |
| CATTTAAATGTTACTACTAC+TGG | 0.355694 | 1:-79541740 | MsG0180004559.01.T01:CDS |
| ATTGTCCTCCTTATGTAGAT+TGG | 0.357399 | 1:-79542688 | MsG0180004559.01.T01:CDS |
| TTTCTTGAACTTCATTCAAA+TGG | 0.360903 | 1:+79542636 | None:intergenic |
| CTAAAAGGATCTTGTGTTTG+TGG | 0.365756 | 1:+79542815 | None:intergenic |
| TTCTTGAACTTCATTCAAAT+GGG | 0.379399 | 1:+79542637 | None:intergenic |
| TCTTTACTCTCTCAGTGTTA+AGG | 0.389543 | 1:-79541708 | MsG0180004559.01.T01:intron |
| GTTGAATCCTATTGACCATC+TGG | 0.393376 | 1:+79541524 | None:intergenic |
| CTGTATACCAGATGGTCAAT+AGG | 0.406963 | 1:-79541531 | MsG0180004559.01.T01:CDS |
| ACCCTTTGGATGTAAGAATC+TGG | 0.440881 | 1:+79542398 | None:intergenic |
| GGTTGAAGATGATTGCAAGT+TGG | 0.450829 | 1:-79541578 | MsG0180004559.01.T01:CDS |
| TTGTTATTCCAATCTACATA+AGG | 0.465642 | 1:+79542680 | None:intergenic |
| TTAAAATAAGATCAAGAAAG+AGG | 0.490885 | 1:-79541820 | MsG0180004559.01.T01:CDS |
| TGCCAGATTCTTACATCCAA+AGG | 0.505226 | 1:-79542400 | MsG0180004559.01.T01:CDS |
| GCCAGATTCTTACATCCAAA+GGG | 0.516817 | 1:-79542399 | MsG0180004559.01.T01:intron |
| TTAGTAATAGTATTACTACT+TGG | 0.517777 | 1:+79542602 | None:intergenic |
| TGTTATGTGTTTACCTGAAG+AGG | 0.521964 | 1:-79542882 | None:intergenic |
| ACACAGTTATTCGTCCGAAA+AGG | 0.534814 | 1:-79542544 | MsG0180004559.01.T01:CDS |
| GATCAAGCTTCTATAATTGG+AGG | 0.557225 | 1:-79542088 | MsG0180004559.01.T01:CDS |
| TGAACTTCATTCAAATGGGG+TGG | 0.566537 | 1:+79542641 | None:intergenic |
| ATTACTACTTGGATCAGTAG+TGG | 0.576946 | 1:+79542613 | None:intergenic |
| TGATTGCAAGTTGGGATCAG+TGG | 0.579380 | 1:-79541569 | MsG0180004559.01.T01:CDS |
| TTATCACTTGTTGAATACTG+TGG | 0.584055 | 1:+79541938 | None:intergenic |
| GGTCAATAGGATTCAACAAG+AGG | 0.595940 | 1:-79541518 | MsG0180004559.01.T01:CDS |
| AGCTTCAGCTGTATACCAGA+TGG | 0.597796 | 1:-79541539 | MsG0180004559.01.T01:CDS |
| TATGTAGATTGGAATAACAA+TGG | 0.602597 | 1:-79542677 | MsG0180004559.01.T01:CDS |
| TTATTCCAATCTACATAAGG+AGG | 0.614549 | 1:+79542683 | None:intergenic |
| TCTTGAACTTCATTCAAATG+GGG | 0.614700 | 1:+79542638 | None:intergenic |
| ACGAATAACTGTGTTCACAG+AGG | 0.614740 | 1:+79542553 | None:intergenic |
| CGAATAACTGTGTTCACAGA+GGG | 0.620805 | 1:+79542554 | None:intergenic |
| AGACATGGCACTAGAAGCAG+TGG | 0.632855 | 1:-79542843 | MsG0180004559.01.T01:CDS |
| AAGAGATAGAGTGAAAGACA+TGG | 0.656321 | 1:-79542858 | MsG0180004559.01.T01:CDS |
| GTGAAATCTGATGAAGCTGG+TGG | 0.684533 | 1:-79541998 | MsG0180004559.01.T01:CDS |
| TGCTGACATAGAAGTGACAA+TGG | 0.687267 | 1:-79541861 | MsG0180004559.01.T01:CDS |
| GAAGTGAAATCTGATGAAGC+TGG | 0.693568 | 1:-79542001 | MsG0180004559.01.T01:CDS |
| TGACATAGAAGTGACAATGG+TGG | 0.780182 | 1:-79541858 | MsG0180004559.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GCATTGAATTTTTTTTATTT+AGG | - | Chr1:79542783-79542802 | MsG0180004559.01.T01:CDS | 15.0% |
| !! | AATGAAAAGAATTCAGAAAA+TGG | + | Chr1:79542423-79542442 | None:intergenic | 20.0% |
| !! | TTAAAATAAGATCAAGAAAG+AGG | - | Chr1:79542562-79542581 | MsG0180004559.01.T01:CDS | 20.0% |
| !! | TTAGTAATAGTATTACTACT+TGG | + | Chr1:79541783-79541802 | None:intergenic | 20.0% |
| !!! | AGATTTCTTTGAACAATTTT+TGG | - | Chr1:79542737-79542756 | MsG0180004559.01.T01:CDS | 20.0% |
| !!! | ATCTTATTTTAAGATTTGCA+TGG | + | Chr1:79542554-79542573 | None:intergenic | 20.0% |
| !!! | TGTTTGTTTTTTTTTGTTGT+TGG | - | Chr1:79542259-79542278 | MsG0180004559.01.T01:intron | 20.0% |
| ! | CATTTAAATGTTACTACTAC+TGG | - | Chr1:79542642-79542661 | MsG0180004559.01.T01:CDS | 25.0% |
| ! | TATGTAGATTGGAATAACAA+TGG | - | Chr1:79541705-79541724 | MsG0180004559.01.T01:intron | 25.0% |
| ! | TCTTTGATTCCATAACTAAA+AGG | + | Chr1:79541585-79541604 | None:intergenic | 25.0% |
| ! | TTCTTGAACTTCATTCAAAT+GGG | + | Chr1:79541748-79541767 | None:intergenic | 25.0% |
| ! | TTTCTTGAACTTCATTCAAA+TGG | + | Chr1:79541749-79541768 | None:intergenic | 25.0% |
| !! | TTGTTATTCCAATCTACATA+AGG | + | Chr1:79541705-79541724 | None:intergenic | 25.0% |
| TCTTGAACTTCATTCAAATG+GGG | + | Chr1:79541747-79541766 | None:intergenic | 30.0% | |
| TTATTCCAATCTACATAAGG+AGG | + | Chr1:79541702-79541721 | None:intergenic | 30.0% | |
| ! | GAGGACAATTATTTTCTGTT+TGG | + | Chr1:79541683-79541702 | None:intergenic | 30.0% |
| ! | TTATCACTTGTTGAATACTG+TGG | + | Chr1:79542447-79542466 | None:intergenic | 30.0% |
| !! | ACACAAGATCCTTTTAGTTA+TGG | - | Chr1:79541573-79541592 | MsG0180004559.01.T01:CDS | 30.0% |
| AACATGCAGTTACATGTTCT+TGG | - | Chr1:79542156-79542175 | MsG0180004559.01.T01:intron | 35.0% | |
| AAGAGATAGAGTGAAAGACA+TGG | - | Chr1:79541524-79541543 | MsG0180004559.01.T01:CDS | 35.0% | |
| ATTACTACTTGGATCAGTAG+TGG | + | Chr1:79541772-79541791 | None:intergenic | 35.0% | |
| ATTGTCCTCCTTATGTAGAT+TGG | - | Chr1:79541694-79541713 | MsG0180004559.01.T01:intron | 35.0% | |
| CAGTGAGTTTAGTAGAATCA+CGG | - | Chr1:79542052-79542071 | MsG0180004559.01.T01:CDS | 35.0% | |
| CTAAAAGGATCTTGTGTTTG+TGG | + | Chr1:79541570-79541589 | None:intergenic | 35.0% | |
| GATCAAGCTTCTATAATTGG+AGG | - | Chr1:79542294-79542313 | MsG0180004559.01.T01:intron | 35.0% | |
| GCTACTATTGGTGAAAAAGT+TGG | - | Chr1:79542480-79542499 | MsG0180004559.01.T01:CDS | 35.0% | |
| GGTGATCAAGCTTCTATAAT+TGG | - | Chr1:79542291-79542310 | MsG0180004559.01.T01:intron | 35.0% | |
| GTTGAAGATGATTGCAAGTT+GGG | - | Chr1:79542805-79542824 | MsG0180004559.01.T01:CDS | 35.0% | |
| TCTAATTCTGCAGCTACTAT+TGG | - | Chr1:79542468-79542487 | MsG0180004559.01.T01:CDS | 35.0% | |
| TCTTTACTCTCTCAGTGTTA+AGG | - | Chr1:79542674-79542693 | MsG0180004559.01.T01:CDS | 35.0% | |
| TGTAAGTAGTAAGTACCCTT+TGG | + | Chr1:79542001-79542020 | None:intergenic | 35.0% | |
| ! | AGCTCTGGTAATCTAAAGTT+CGG | + | Chr1:79542192-79542211 | None:intergenic | 35.0% |
| ! | GAGCACAAGTTTCATTTTCT+TGG | - | Chr1:79542342-79542361 | MsG0180004559.01.T01:intron | 35.0% |
| !! | ACCATTTTCAAGAGCTGTTT+TGG | + | Chr1:79542588-79542607 | None:intergenic | 35.0% |
| !!! | TTTTGTTGTTGGTGAATGCA+GGG | - | Chr1:79542270-79542289 | MsG0180004559.01.T01:intron | 35.0% |
| !!! | TTTTTGTTGTTGGTGAATGC+AGG | - | Chr1:79542269-79542288 | MsG0180004559.01.T01:intron | 35.0% |
| ACACAGTTATTCGTCCGAAA+AGG | - | Chr1:79541838-79541857 | MsG0180004559.01.T01:CDS | 40.0% | |
| ACCCTTTGGATGTAAGAATC+TGG | + | Chr1:79541987-79542006 | None:intergenic | 40.0% | |
| ACGAATAACTGTGTTCACAG+AGG | + | Chr1:79541832-79541851 | None:intergenic | 40.0% | |
| ACTCTATCTCTTTCCTCTTC+AGG | + | Chr1:79541516-79541535 | None:intergenic | 40.0% | |
| CAGCTCTTGAAAATGGTATC+TGG | - | Chr1:79542591-79542610 | MsG0180004559.01.T01:CDS | 40.0% | |
| CGAATAACTGTGTTCACAGA+GGG | + | Chr1:79541831-79541850 | None:intergenic | 40.0% | |
| CTGTATACCAGATGGTCAAT+AGG | - | Chr1:79542851-79542870 | MsG0180004559.01.T01:CDS | 40.0% | |
| GAAGTGAAATCTGATGAAGC+TGG | - | Chr1:79542381-79542400 | MsG0180004559.01.T01:intron | 40.0% | |
| GCCAAAACAGCTCTTGAAAA+TGG | - | Chr1:79542584-79542603 | MsG0180004559.01.T01:CDS | 40.0% | |
| GCCAGATTCTTACATCCAAA+GGG | - | Chr1:79541983-79542002 | MsG0180004559.01.T01:CDS | 40.0% | |
| GGTCAATAGGATTCAACAAG+AGG | - | Chr1:79542864-79542883 | MsG0180004559.01.T01:CDS | 40.0% | |
| GGTTGAAGATGATTGCAAGT+TGG | - | Chr1:79542804-79542823 | MsG0180004559.01.T01:CDS | 40.0% | |
| GTTGAATCCTATTGACCATC+TGG | + | Chr1:79542861-79542880 | None:intergenic | 40.0% | |
| TGAACTTCATTCAAATGGGG+TGG | + | Chr1:79541744-79541763 | None:intergenic | 40.0% | |
| TGACATAGAAGTGACAATGG+TGG | - | Chr1:79542524-79542543 | MsG0180004559.01.T01:CDS | 40.0% | |
| TGCCAGATTCTTACATCCAA+AGG | - | Chr1:79541982-79542001 | MsG0180004559.01.T01:CDS | 40.0% | |
| TGCTGACATAGAAGTGACAA+TGG | - | Chr1:79542521-79542540 | MsG0180004559.01.T01:CDS | 40.0% | |
| ! | CCCTCTAGTTTCTAGAAGAA+GGG | + | Chr1:79542215-79542234 | None:intergenic | 40.0% |
| ! | CCCTTCTTCTAGAAACTAGA+GGG | - | Chr1:79542212-79542231 | MsG0180004559.01.T01:intron | 40.0% |
| ! | TCCCTTCTTCTAGAAACTAG+AGG | - | Chr1:79542211-79542230 | MsG0180004559.01.T01:intron | 40.0% |
| AGCTTCAGCTGTATACCAGA+TGG | - | Chr1:79542843-79542862 | MsG0180004559.01.T01:CDS | 45.0% | |
| CAAATGGGGTGGTGAAAAAG+TGG | + | Chr1:79541733-79541752 | None:intergenic | 45.0% | |
| GTGAAATCTGATGAAGCTGG+TGG | - | Chr1:79542384-79542403 | MsG0180004559.01.T01:intron | 45.0% | |
| ! | GCCCTCTAGTTTCTAGAAGA+AGG | + | Chr1:79542216-79542235 | None:intergenic | 45.0% |
| ! | TGATTGCAAGTTGGGATCAG+TGG | - | Chr1:79542813-79542832 | MsG0180004559.01.T01:CDS | 45.0% |
| ! | TTTCTAGAAGAAGGGAGCTC+TGG | + | Chr1:79542207-79542226 | None:intergenic | 45.0% |
| AGACATGGCACTAGAAGCAG+TGG | - | Chr1:79541539-79541558 | MsG0180004559.01.T01:CDS | 50.0% | |
| !!! | CTTCTAGCTCTGCGCCTTTT+CGG | + | Chr1:79541855-79541874 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 79541504 | 79542900 | 79541504 | ID=MsG0180004559.01;Name=MsG0180004559.01 |
| Chr1 | mRNA | 79541504 | 79542900 | 79541504 | ID=MsG0180004559.01.T01;Parent=MsG0180004559.01;Name=MsG0180004559.01.T01;_AED=0.29;_eAED=0.32;_QI=0|0|0|1|1|1|3|0|333 |
| Chr1 | exon | 79542400 | 79542900 | 79542400 | ID=MsG0180004559.01.T01:exon:45719;Parent=MsG0180004559.01.T01 |
| Chr1 | exon | 79541709 | 79542113 | 79541709 | ID=MsG0180004559.01.T01:exon:45718;Parent=MsG0180004559.01.T01 |
| Chr1 | exon | 79541504 | 79541599 | 79541504 | ID=MsG0180004559.01.T01:exon:45717;Parent=MsG0180004559.01.T01 |
| Chr1 | CDS | 79542400 | 79542900 | 79542400 | ID=MsG0180004559.01.T01:cds;Parent=MsG0180004559.01.T01 |
| Chr1 | CDS | 79541709 | 79542113 | 79541709 | ID=MsG0180004559.01.T01:cds;Parent=MsG0180004559.01.T01 |
| Chr1 | CDS | 79541504 | 79541599 | 79541504 | ID=MsG0180004559.01.T01:cds;Parent=MsG0180004559.01.T01 |
| Gene Sequence |
| Protein sequence |
