Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004027.01.T01 | AES60997.1 | 95.513 | 312 | 13 | 1 | 1 | 311 | 1 | 312 | 0 | 603 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004027.01.T01 | Q6NM73 | 67.23 | 296 | 89 | 2 | 8 | 297 | 10 | 303 | 2.79E-134 | 386 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004027.01.T01 | G7I3U3 | 95.513 | 312 | 13 | 1 | 1 | 311 | 1 | 312 | 0.0 | 603 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048842.01 | MsG0180004027.01 | -0.805596 | 1.236559e-49 | 6.568585e-47 |
| MsG0180000076.01 | MsG0180004027.01 | 0.815230 | 1.028355e-51 | 7.049356e-49 |
| MsG0180001272.01 | MsG0180004027.01 | 0.801758 | 7.739958e-49 | 3.726223e-46 |
| MsG0180002984.01 | MsG0180004027.01 | 0.805406 | 1.355836e-49 | 7.167254e-47 |
| MsG0180004027.01 | MsG0180004126.01 | 0.866018 | 3.875050e-65 | 1.286877e-61 |
| MsG0180004027.01 | MsG0180004510.01 | 0.905885 | 2.673745e-80 | 4.417969e-76 |
| MsG0180004027.01 | MsG0180004559.01 | 0.804549 | 2.048314e-49 | 1.059114e-46 |
| MsG0180004027.01 | MsG0180004989.01 | 0.816381 | 5.695212e-52 | 4.029245e-49 |
| MsG0180004027.01 | MsG0180005272.01 | 0.824110 | 9.640780e-54 | 8.459568e-51 |
| MsG0180004027.01 | MsG0180005667.01 | 0.860292 | 2.285971e-63 | 6.217085e-60 |
| MsG0180004027.01 | MsG0180006026.01 | 0.843078 | 1.747902e-58 | 2.703523e-55 |
| MsG0180004027.01 | MsG0180006182.01 | 0.874940 | 4.559642e-68 | 2.091260e-64 |
| MsG0180004027.01 | MsG0280006884.01 | 0.815343 | 9.703142e-52 | 6.672548e-49 |
| MsG0180004027.01 | MsG0280007419.01 | 0.875337 | 3.336957e-68 | 1.552051e-64 |
| MsG0180004027.01 | MsG0280007667.01 | 0.802760 | 4.815518e-49 | 2.378337e-46 |
| MsG0180004027.01 | MsG0280007983.01 | 0.845677 | 3.497582e-59 | 5.874344e-56 |
| MsG0180004027.01 | MsG0280009241.01 | 0.855946 | 4.484934e-62 | 1.053152e-58 |
| MsG0180004027.01 | MsG0280011331.01 | 0.806499 | 7.983971e-50 | 4.340220e-47 |
| MsG0180004027.01 | MsG0380013041.01 | 0.827790 | 1.287512e-54 | 1.255677e-51 |
| MsG0180004027.01 | MsG0380015371.01 | 0.805203 | 1.495064e-49 | 7.862135e-47 |
| MsG0180004027.01 | MsG0380015641.01 | 0.804344 | 2.259475e-49 | 1.162196e-46 |
| MsG0180004027.01 | MsG0380015871.01 | 0.844992 | 5.361465e-59 | 8.808080e-56 |
| MsG0180004027.01 | MsG0380016196.01 | -0.800647 | 1.306253e-48 | 6.114749e-46 |
| MsG0180004027.01 | MsG0380016217.01 | 0.814736 | 1.323852e-51 | 8.954607e-49 |
| MsG0180004027.01 | MsG0380016363.01 | 0.810448 | 1.146955e-50 | 6.915380e-48 |
| MsG0180004027.01 | MsG0380016856.01 | 0.806558 | 7.759334e-50 | 4.224576e-47 |
| MsG0180004027.01 | MsG0380017240.01 | 0.842666 | 2.249526e-58 | 3.434900e-55 |
| MsG0180004027.01 | MsG0380017361.01 | 0.812591 | 3.924886e-51 | 2.505623e-48 |
| MsG0180004027.01 | MsG0380017858.01 | 0.805966 | 1.034273e-49 | 5.546692e-47 |
| MsG0180004027.01 | MsG0380017997.01 | 0.842022 | 3.331857e-58 | 4.985930e-55 |
| MsG0180004027.01 | MsG0380018007.01 | 0.821865 | 3.217026e-53 | 2.649052e-50 |
| MsG0180004027.01 | MsG0480018256.01 | 0.803278 | 3.762170e-49 | 1.882797e-46 |
| MsG0180004027.01 | MsG0480018526.01 | 0.803667 | 3.125400e-49 | 1.579786e-46 |
| MsG0180004027.01 | MsG0480018567.01 | 0.873963 | 9.778816e-68 | 4.324574e-64 |
| MsG0180004027.01 | MsG0480018699.01 | 0.802762 | 4.809727e-49 | 2.375649e-46 |
| MsG0180004027.01 | MsG0480021958.01 | 0.855886 | 4.668464e-62 | 1.093910e-58 |
| MsG0180004027.01 | MsG0480022167.01 | 0.824880 | 6.350923e-54 | 5.697960e-51 |
| MsG0180004027.01 | MsG0480022407.01 | 0.816990 | 4.157647e-52 | 2.991129e-49 |
| MsG0180004027.01 | MsG0480023324.01 | 0.902101 | 1.366796e-78 | 1.904654e-74 |
| MsG0180004027.01 | MsG0480023450.01 | 0.843192 | 1.629483e-58 | 2.529565e-55 |
| MsG0180004027.01 | MsG0480023484.01 | 0.839481 | 1.543948e-57 | 2.135032e-54 |
| MsG0180004027.01 | MsG0480023698.01 | 0.810793 | 9.658749e-51 | 5.877767e-48 |
| MsG0180004027.01 | MsG0480023706.01 | 0.805448 | 1.328465e-49 | 7.030102e-47 |
| MsG0180004027.01 | MsG0480023944.01 | 0.813789 | 2.142110e-51 | 1.412273e-48 |
| MsG0180004027.01 | MsG0580024119.01 | 0.807671 | 4.511466e-50 | 2.527986e-47 |
| MsG0180004027.01 | MsG0580024144.01 | -0.807940 | 3.954154e-50 | 2.231348e-47 |
| MsG0180004027.01 | MsG0580024478.01 | 0.811612 | 6.418699e-51 | 3.991245e-48 |
| MsG0180004027.01 | MsG0580024657.01 | 0.804902 | 1.727986e-49 | 9.017876e-47 |
| MsG0180004027.01 | MsG0580024954.01 | 0.814193 | 1.745167e-51 | 1.163218e-48 |
| MsG0180004027.01 | MsG0580025093.01 | 0.837271 | 5.736106e-57 | 7.412193e-54 |
| MsG0180004027.01 | MsG0580025385.01 | 0.849925 | 2.364161e-60 | 4.554106e-57 |
| MsG0180004027.01 | MsG0580025464.01 | 0.812341 | 4.450706e-51 | 2.822593e-48 |
| MsG0180004027.01 | MsG0580027938.01 | 0.800869 | 1.177220e-48 | 5.541865e-46 |
| MsG0180004027.01 | MsG0580028228.01 | 0.830116 | 3.519879e-55 | 3.674137e-52 |
| MsG0180004027.01 | MsG0580028330.01 | 0.834884 | 2.314892e-56 | 2.783968e-53 |
| MsG0180004027.01 | MsG0580029591.01 | 0.803217 | 3.872578e-49 | 1.935063e-46 |
| MsG0180004027.01 | MsG0580030179.01 | 0.852999 | 3.192707e-61 | 6.796820e-58 |
| MsG0180004027.01 | MsG0680031616.01 | 0.874197 | 8.152686e-68 | 3.636759e-64 |
| MsG0180004027.01 | MsG0680031686.01 | 0.823946 | 1.053311e-53 | 9.201053e-51 |
| MsG0180004027.01 | MsG0680033405.01 | 0.815785 | 7.736303e-52 | 5.385187e-49 |
| MsG0180004027.01 | MsG0780037232.01 | 0.829185 | 5.929101e-55 | 6.023927e-52 |
| MsG0180004027.01 | MsG0780038393.01 | 0.828391 | 9.228669e-55 | 9.160734e-52 |
| MsG0180004027.01 | MsG0780038445.01 | 0.807688 | 4.473634e-50 | 2.507940e-47 |
| MsG0180004027.01 | MsG0780038810.01 | 0.823845 | 1.112428e-53 | 9.689594e-51 |
| MsG0180004027.01 | MsG0780039491.01 | 0.813576 | 2.387057e-51 | 1.564712e-48 |
| MsG0180004027.01 | MsG0780040118.01 | 0.820343 | 7.211778e-53 | 5.691320e-50 |
| MsG0180004027.01 | MsG0780040139.01 | 0.804611 | 1.988009e-49 | 1.029599e-46 |
| MsG0180004027.01 | MsG0780040364.01 | 0.870438 | 1.458706e-66 | 5.674232e-63 |
| MsG0180004027.01 | MsG0780040483.01 | 0.809077 | 2.261595e-50 | 1.315111e-47 |
| MsG0180004027.01 | MsG0780040543.01 | 0.820485 | 6.692073e-53 | 5.301895e-50 |
| MsG0180004027.01 | MsG0780040564.01 | 0.866757 | 2.257775e-65 | 7.696615e-62 |
| MsG0180004027.01 | MsG0780040905.01 | 0.806241 | 9.049134e-50 | 4.887254e-47 |
| MsG0180004027.01 | MsG0780040990.01 | 0.806668 | 7.355182e-50 | 4.015489e-47 |
| MsG0180004027.01 | MsG0780041141.01 | 0.811685 | 6.187468e-51 | 3.854941e-48 |
| MsG0180004027.01 | MsG0780041303.01 | 0.808316 | 3.288061e-50 | 1.874092e-47 |
| MsG0180004027.01 | MsG0780041354.01 | 0.803281 | 3.757636e-49 | 1.880627e-46 |
| MsG0180004027.01 | MsG0780041363.01 | 0.881619 | 2.064688e-70 | 1.218198e-66 |
| MsG0180004027.01 | MsG0780041548.01 | 0.841554 | 4.428084e-58 | 6.530474e-55 |
| MsG0180004027.01 | MsG0780041565.01 | 0.820280 | 7.455530e-53 | 5.873407e-50 |
| MsG0180004027.01 | MsG0880042927.01 | 0.820428 | 6.896941e-53 | 5.455843e-50 |
| MsG0180004027.01 | MsG0880043297.01 | 0.833274 | 5.860020e-56 | 6.715398e-53 |
| MsG0180004027.01 | MsG0880043431.01 | 0.828382 | 9.274175e-55 | 9.203513e-52 |
| MsG0180004027.01 | MsG0880045584.01 | 0.813027 | 3.150263e-51 | 2.034394e-48 |
| MsG0180004027.01 | MsG0880045963.01 | 0.813376 | 2.641358e-51 | 1.722080e-48 |
| MsG0180004027.01 | MsG0880046337.01 | 0.801209 | 1.003025e-48 | 4.762546e-46 |
| MsG0180004027.01 | MsG0880046906.01 | 0.803772 | 2.971514e-49 | 1.506136e-46 |
| MsG0180004027.01 | MsG0880046983.01 | 0.819065 | 1.412233e-52 | 1.075572e-49 |
| MsG0180004027.01 | MsG0880046991.01 | 0.800990 | 1.111879e-48 | 5.250682e-46 |
| MsG0180004027.01 | MsG0880047466.01 | 0.845672 | 3.508652e-59 | 5.891889e-56 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004027.01.T01 | MTR_1g073320 | 95.513 | 312 | 13 | 1 | 1 | 311 | 1 | 312 | 0.0 | 603 |
| MsG0180004027.01.T01 | MTR_1g044580 | 69.868 | 302 | 75 | 3 | 1 | 298 | 1 | 290 | 9.18e-142 | 416 |
| MsG0180004027.01.T01 | MTR_7g098410 | 48.789 | 289 | 131 | 4 | 11 | 298 | 10 | 282 | 9.71e-89 | 267 |
| MsG0180004027.01.T01 | MTR_1g080350 | 39.130 | 299 | 156 | 9 | 12 | 298 | 10 | 294 | 3.70e-63 | 202 |
| MsG0180004027.01.T01 | MTR_5g078030 | 29.553 | 291 | 185 | 5 | 9 | 297 | 4 | 276 | 4.08e-34 | 126 |
| MsG0180004027.01.T01 | MTR_3g065370 | 30.822 | 292 | 172 | 6 | 6 | 293 | 5 | 270 | 9.92e-34 | 125 |
| MsG0180004027.01.T01 | MTR_8g104990 | 28.881 | 277 | 182 | 5 | 26 | 299 | 18 | 282 | 1.88e-30 | 116 |
| MsG0180004027.01.T01 | MTR_1g044560 | 43.299 | 97 | 55 | 0 | 144 | 240 | 28 | 124 | 1.98e-18 | 80.1 |
| MsG0180004027.01.T01 | MTR_7g052020 | 22.835 | 254 | 158 | 4 | 8 | 241 | 4 | 239 | 1.41e-16 | 78.2 |
| MsG0180004027.01.T01 | MTR_1g104890 | 25.769 | 260 | 166 | 8 | 47 | 290 | 54 | 302 | 3.86e-16 | 78.2 |
| MsG0180004027.01.T01 | MTR_1g104890 | 25.769 | 260 | 166 | 8 | 47 | 290 | 54 | 302 | 1.76e-15 | 77.0 |
| MsG0180004027.01.T01 | MTR_1g104890 | 25.769 | 260 | 166 | 8 | 47 | 290 | 54 | 302 | 2.00e-15 | 77.0 |
| MsG0180004027.01.T01 | MTR_1g104890 | 25.769 | 260 | 166 | 8 | 47 | 290 | 54 | 302 | 2.85e-15 | 76.6 |
| MsG0180004027.01.T01 | MTR_1g105615 | 25.101 | 247 | 160 | 9 | 9 | 237 | 11 | 250 | 1.30e-14 | 74.3 |
| MsG0180004027.01.T01 | MTR_6g463830 | 24.149 | 323 | 188 | 11 | 1 | 281 | 1 | 308 | 2.66e-14 | 73.9 |
| MsG0180004027.01.T01 | MTR_5g005480 | 26.168 | 214 | 143 | 4 | 38 | 237 | 35 | 247 | 7.61e-14 | 72.4 |
| MsG0180004027.01.T01 | MTR_3g064080 | 27.679 | 224 | 149 | 5 | 21 | 234 | 18 | 238 | 1.42e-13 | 71.6 |
| MsG0180004027.01.T01 | MTR_6g055960 | 23.462 | 260 | 159 | 5 | 44 | 270 | 33 | 285 | 1.58e-13 | 71.6 |
| MsG0180004027.01.T01 | MTR_6g463400 | 23.108 | 251 | 175 | 5 | 1 | 237 | 1 | 247 | 2.06e-13 | 70.9 |
| MsG0180004027.01.T01 | MTR_5g005530 | 23.554 | 242 | 160 | 6 | 68 | 292 | 68 | 301 | 1.31e-12 | 68.6 |
| MsG0180004027.01.T01 | MTR_5g068190 | 25.103 | 243 | 160 | 9 | 9 | 236 | 9 | 244 | 4.52e-12 | 67.0 |
| MsG0180004027.01.T01 | MTR_6g463710 | 26.050 | 238 | 143 | 7 | 23 | 237 | 22 | 249 | 7.17e-12 | 66.6 |
| MsG0180004027.01.T01 | MTR_6g057770 | 26.050 | 238 | 143 | 7 | 23 | 237 | 22 | 249 | 7.86e-12 | 66.6 |
| MsG0180004027.01.T01 | MTR_1g105650 | 25.541 | 231 | 135 | 7 | 32 | 237 | 33 | 251 | 1.01e-11 | 65.9 |
| MsG0180004027.01.T01 | MTR_3g011910 | 23.843 | 281 | 171 | 10 | 5 | 252 | 10 | 280 | 6.89e-11 | 63.2 |
| MsG0180004027.01.T01 | MTR_6g053000 | 25.000 | 240 | 161 | 7 | 11 | 236 | 6 | 240 | 7.19e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004027.01.T01 | AT1G04520 | 67.230 | 296 | 89 | 2 | 8 | 297 | 10 | 303 | 2.84e-135 | 386 |
| MsG0180004027.01.T01 | AT2G33330 | 65.116 | 301 | 100 | 3 | 1 | 297 | 1 | 300 | 1.46e-133 | 381 |
| MsG0180004027.01.T01 | AT5G43980 | 44.330 | 291 | 136 | 5 | 25 | 300 | 20 | 299 | 9.96e-78 | 239 |
| MsG0180004027.01.T01 | AT3G04370 | 35.738 | 305 | 154 | 6 | 24 | 298 | 25 | 317 | 1.18e-58 | 191 |
| MsG0180004027.01.T01 | AT3G04370 | 35.197 | 304 | 156 | 5 | 24 | 298 | 25 | 316 | 1.20e-58 | 191 |
| MsG0180004027.01.T01 | AT5G37660 | 31.894 | 301 | 185 | 7 | 11 | 309 | 15 | 297 | 3.56e-35 | 129 |
| MsG0180004027.01.T01 | AT5G37660 | 30.996 | 271 | 168 | 6 | 11 | 279 | 15 | 268 | 5.03e-34 | 126 |
| MsG0180004027.01.T01 | AT3G60720 | 29.044 | 272 | 165 | 6 | 33 | 296 | 29 | 280 | 1.96e-31 | 119 |
| MsG0180004027.01.T01 | AT3G60720 | 28.889 | 270 | 164 | 6 | 33 | 294 | 29 | 278 | 2.01e-30 | 116 |
| MsG0180004027.01.T01 | AT2G01660 | 29.966 | 297 | 185 | 9 | 11 | 300 | 7 | 287 | 4.92e-29 | 112 |
| MsG0180004027.01.T01 | AT2G01660 | 30.201 | 298 | 184 | 10 | 11 | 300 | 7 | 288 | 6.24e-29 | 112 |
| MsG0180004027.01.T01 | AT2G01660 | 31.034 | 232 | 142 | 7 | 26 | 250 | 20 | 240 | 7.45e-26 | 103 |
| MsG0180004027.01.T01 | AT1G70690 | 28.254 | 315 | 186 | 9 | 10 | 310 | 9 | 297 | 9.70e-25 | 101 |
| MsG0180004027.01.T01 | AT3G60720 | 27.885 | 208 | 139 | 4 | 33 | 233 | 29 | 232 | 5.34e-24 | 98.6 |
| MsG0180004027.01.T01 | AT5G48540 | 23.077 | 221 | 156 | 5 | 34 | 241 | 24 | 243 | 1.76e-16 | 78.2 |
| MsG0180004027.01.T01 | AT5G40380 | 27.946 | 297 | 175 | 12 | 9 | 296 | 12 | 278 | 6.10e-15 | 75.5 |
| MsG0180004027.01.T01 | AT5G40380 | 27.946 | 297 | 175 | 12 | 9 | 296 | 12 | 278 | 7.26e-15 | 75.5 |
| MsG0180004027.01.T01 | AT5G40380 | 27.946 | 297 | 175 | 12 | 9 | 296 | 12 | 278 | 7.41e-15 | 75.5 |
| MsG0180004027.01.T01 | AT5G41290 | 24.468 | 282 | 192 | 6 | 6 | 272 | 5 | 280 | 6.74e-13 | 68.2 |
| MsG0180004027.01.T01 | AT4G05200 | 23.127 | 307 | 211 | 10 | 12 | 296 | 11 | 314 | 1.21e-12 | 68.9 |
| MsG0180004027.01.T01 | AT4G05200 | 23.127 | 307 | 211 | 10 | 12 | 296 | 29 | 332 | 1.27e-12 | 68.9 |
Find 55 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGCAAGAGTTCAGCTTTC+AGG | 0.163538 | 1:-72112084 | MsG0180004027.01.T01:CDS |
| TCTGGTTACTCTTCATCTTT+TGG | 0.217123 | 1:-72111670 | MsG0180004027.01.T01:CDS |
| AGATACCTGTGATGCTATTT+TGG | 0.228242 | 1:+72112199 | None:intergenic |
| GAATGTGGAAGTTCTATTTC+AGG | 0.278336 | 1:-72111790 | MsG0180004027.01.T01:CDS |
| AAGACTGTGGCAATAATATT+AGG | 0.324267 | 1:-72111163 | MsG0180004027.01.T01:CDS |
| GGTGGTGGATCATCTTCTTC+TGG | 0.327218 | 1:-72111688 | MsG0180004027.01.T01:CDS |
| CCACCCTTTGGAACCCCATT+AGG | 0.331693 | 1:+72111724 | None:intergenic |
| TAGACCAAAGTTGTGTAATC+AGG | 0.371990 | 1:+72112351 | None:intergenic |
| AAGATGATGATTTAGAGAAA+TGG | 0.375671 | 1:+72112376 | None:intergenic |
| GATTGTGAATATGCACCATT+TGG | 0.394933 | 1:+72112297 | None:intergenic |
| AGATGATGATTTAGAGAAAT+GGG | 0.405156 | 1:+72112377 | None:intergenic |
| TACATGCTGTATGAGGTTGC+TGG | 0.420117 | 1:-72112057 | MsG0180004027.01.T01:CDS |
| AAGGGTGGTCATTCTTCATC+TGG | 0.421635 | 1:-72111709 | MsG0180004027.01.T01:CDS |
| TTCTCCTGATTACACAACTT+TGG | 0.429549 | 1:-72112355 | MsG0180004027.01.T01:CDS |
| AGATTCTTCAAAACAACAAC+TGG | 0.449417 | 1:-72112228 | MsG0180004027.01.T01:CDS |
| GAAGAACAAAAGAAGAAAGA+GGG | 0.456695 | 1:+72112413 | None:intergenic |
| GGTAGGTGTTGTGTTGTACA+TGG | 0.467781 | 1:-72111070 | MsG0180004027.01.T01:CDS |
| AATGGTGTTGTGAGTAATCA+TGG | 0.467963 | 1:-72111925 | MsG0180004027.01.T01:CDS |
| CATAGTTTGTCTGAGAGAAC+TGG | 0.468018 | 1:+72112120 | None:intergenic |
| ACAACAAATGCTGCTGGAAG+AGG | 0.468669 | 1:-72111988 | MsG0180004027.01.T01:CDS |
| GGTCAGTGTGAGGGTGATGT+TGG | 0.474760 | 1:-72111865 | MsG0180004027.01.T01:CDS |
| GATGAAGAATGACCACCCTT+TGG | 0.474944 | 1:+72111712 | None:intergenic |
| TTCAAAACAACAACTGGTAA+TGG | 0.481327 | 1:-72112222 | MsG0180004027.01.T01:CDS |
| AAGCTGAACTCTTGCAGAGA+TGG | 0.484956 | 1:+72112089 | None:intergenic |
| TGAAGAACAAAAGAAGAAAG+AGG | 0.494984 | 1:+72112412 | None:intergenic |
| TTACCAAGCTTTGTATGTGA+TGG | 0.497465 | 1:-72111887 | MsG0180004027.01.T01:CDS |
| TTGTGAGATCTCCTCTGCAT+TGG | 0.514398 | 1:+72112175 | None:intergenic |
| GGTCATTCTTCATCTGGTGG+TGG | 0.515877 | 1:-72111703 | MsG0180004027.01.T01:CDS |
| AATGGCCAAAATAGCATCAC+AGG | 0.519556 | 1:-72112204 | MsG0180004027.01.T01:CDS |
| GACCCATCACATACAAAGCT+TGG | 0.525654 | 1:+72111884 | None:intergenic |
| TACCAAGCTTTGTATGTGAT+GGG | 0.532292 | 1:-72111886 | MsG0180004027.01.T01:CDS |
| GCTATGACAGGACAAAATAC+AGG | 0.532912 | 1:-72111187 | MsG0180004027.01.T01:intron |
| ATCTGCATCCCTGAAACTTG+TGG | 0.533086 | 1:+72112030 | None:intergenic |
| TTATCCTAATGGGGTTCCAA+AGG | 0.535083 | 1:-72111728 | MsG0180004027.01.T01:CDS |
| AGGTTGTTACATGCTGTATG+AGG | 0.538390 | 1:-72112064 | MsG0180004027.01.T01:CDS |
| GATGTTGGTGATTCTGATTG+TGG | 0.539277 | 1:-72111850 | MsG0180004027.01.T01:CDS |
| TATCCTAATGGGGTTCCAAA+GGG | 0.541401 | 1:-72111727 | MsG0180004027.01.T01:CDS |
| GAAACATTCACAGATCCAAA+TGG | 0.542666 | 1:-72112312 | MsG0180004027.01.T01:CDS |
| TTATATGCTTGCTATTTGCA+AGG | 0.544026 | 1:-72111114 | MsG0180004027.01.T01:CDS |
| TGTGGAACAACAAATGCTGC+TGG | 0.551763 | 1:-72111994 | MsG0180004027.01.T01:CDS |
| TACACAACTTTGGTCTACAA+AGG | 0.569043 | 1:-72112345 | MsG0180004027.01.T01:CDS |
| CAACAAATGCTGCTGGAAGA+GGG | 0.576463 | 1:-72111987 | MsG0180004027.01.T01:CDS |
| TATGTGATGGGTCAGTGTGA+GGG | 0.578918 | 1:-72111874 | MsG0180004027.01.T01:CDS |
| GGTGGTCATTCTTCATCTGG+TGG | 0.587392 | 1:-72111706 | MsG0180004027.01.T01:CDS |
| GTATGTGATGGGTCAGTGTG+AGG | 0.592839 | 1:-72111875 | MsG0180004027.01.T01:CDS |
| AAGAACAAAAGAAGAAAGAG+GGG | 0.594004 | 1:+72112414 | None:intergenic |
| CAAAGAGCTCAAGTTGAATG+TGG | 0.597170 | 1:-72111805 | MsG0180004027.01.T01:CDS |
| ATTAGGAGGTATAGCTGCTG+TGG | 0.619016 | 1:-72111146 | MsG0180004027.01.T01:CDS |
| CCTAATGGGGTTCCAAAGGG+TGG | 0.628137 | 1:-72111724 | MsG0180004027.01.T01:CDS |
| ACAGGTATCTTCCAATGCAG+AGG | 0.638244 | 1:-72112186 | MsG0180004027.01.T01:CDS |
| ACTGTGGCAATAATATTAGG+AGG | 0.641949 | 1:-72111160 | MsG0180004027.01.T01:CDS |
| TGATGAAGAAACGTGATGGT+AGG | 0.645756 | 1:-72111087 | MsG0180004027.01.T01:CDS |
| AATTTGATGAAGAAACGTGA+TGG | 0.662013 | 1:-72111091 | MsG0180004027.01.T01:CDS |
| GTTCTCTCAGACAAACTATG+TGG | 0.667348 | 1:-72112117 | MsG0180004027.01.T01:CDS |
| ACAAAATACAGGAAAGACTG+TGG | 0.709113 | 1:-72111176 | MsG0180004027.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CAATTTTCTCAAATTATTAT+CGG | + | Chr1:72112004-72112023 | None:intergenic | 15.0% |
| !! | GTTTCATACATATTAGTAAA+TGG | + | Chr1:72112240-72112259 | None:intergenic | 20.0% |
| !! | TATATATAGTTTCACAAAGA+AGG | - | Chr1:72112132-72112151 | MsG0180004027.01.T01:CDS | 20.0% |
| !!! | AGTTTTAGTTATTATCCTAA+TGG | - | Chr1:72111744-72111763 | MsG0180004027.01.T01:CDS | 20.0% |
| !!! | GTTTTAGTTATTATCCTAAT+GGG | - | Chr1:72111745-72111764 | MsG0180004027.01.T01:CDS | 20.0% |
| !!! | TTTTAGTTATTATCCTAATG+GGG | - | Chr1:72111746-72111765 | MsG0180004027.01.T01:CDS | 20.0% |
| ! | AAGATGATGATTTAGAGAAA+TGG | + | Chr1:72111110-72111129 | None:intergenic | 25.0% |
| ! | AGATGATGATTTAGAGAAAT+GGG | + | Chr1:72111109-72111128 | None:intergenic | 25.0% |
| ! | TCAGAATCAATAGATAAGAT+TGG | - | Chr1:72111860-72111879 | MsG0180004027.01.T01:CDS | 25.0% |
| !!! | ATAAGATTGGTTTCAATTCT+GGG | - | Chr1:72111873-72111892 | MsG0180004027.01.T01:CDS | 25.0% |
| !!! | TATCTATTGATTCTGAATGA+TGG | + | Chr1:72111856-72111875 | None:intergenic | 25.0% |
| !!! | TTGTACATGGTTTTACTATA+TGG | - | Chr1:72112426-72112445 | MsG0180004027.01.T01:CDS | 25.0% |
| !!! | TTTGAAGAATCTTGTTTTTG+TGG | + | Chr1:72111247-72111266 | None:intergenic | 25.0% |
| AAGAACAAAAGAAGAAAGAG+GGG | + | Chr1:72111072-72111091 | None:intergenic | 30.0% | |
| AAGACTGTGGCAATAATATT+AGG | - | Chr1:72112320-72112339 | MsG0180004027.01.T01:CDS | 30.0% | |
| AATTTGATGAAGAAACGTGA+TGG | - | Chr1:72112392-72112411 | MsG0180004027.01.T01:CDS | 30.0% | |
| AGATTCTTCAAAACAACAAC+TGG | - | Chr1:72111255-72111274 | MsG0180004027.01.T01:intron | 30.0% | |
| GAAGAACAAAAGAAGAAAGA+GGG | + | Chr1:72111073-72111092 | None:intergenic | 30.0% | |
| TCTCAAATTATTATCGGTGT+CGG | + | Chr1:72111998-72112017 | None:intergenic | 30.0% | |
| TGAAGAACAAAAGAAGAAAG+AGG | + | Chr1:72111074-72111093 | None:intergenic | 30.0% | |
| TTCAAAACAACAACTGGTAA+TGG | - | Chr1:72111261-72111280 | MsG0180004027.01.T01:intron | 30.0% | |
| ! | ATTAATCATTGAGTTGTGTG+TGG | - | Chr1:72112264-72112283 | MsG0180004027.01.T01:CDS | 30.0% |
| !! | GATAAGATTGGTTTCAATTC+TGG | - | Chr1:72111872-72111891 | MsG0180004027.01.T01:CDS | 30.0% |
| !! | TTATATGCTTGCTATTTGCA+AGG | - | Chr1:72112369-72112388 | MsG0180004027.01.T01:CDS | 30.0% |
| !! | TTTTGTGGATTGTGAAACTA+AGG | + | Chr1:72111232-72111251 | None:intergenic | 30.0% |
| ACAAAATACAGGAAAGACTG+TGG | - | Chr1:72112307-72112326 | MsG0180004027.01.T01:CDS | 35.0% | |
| ACCTTAACACTTCAGATTGA+AGG | + | Chr1:72112112-72112131 | None:intergenic | 35.0% | |
| ACTGTGGCAATAATATTAGG+AGG | - | Chr1:72112323-72112342 | MsG0180004027.01.T01:CDS | 35.0% | |
| GAAACATTCACAGATCCAAA+TGG | - | Chr1:72111171-72111190 | MsG0180004027.01.T01:CDS | 35.0% | |
| GAATGTGGAAGTTCTATTTC+AGG | - | Chr1:72111693-72111712 | MsG0180004027.01.T01:CDS | 35.0% | |
| GATTGTGAATATGCACCATT+TGG | + | Chr1:72111189-72111208 | None:intergenic | 35.0% | |
| TACACAACTTTGGTCTACAA+AGG | - | Chr1:72111138-72111157 | MsG0180004027.01.T01:CDS | 35.0% | |
| TAGACCAAAGTTGTGTAATC+AGG | + | Chr1:72111135-72111154 | None:intergenic | 35.0% | |
| TTCTCCTGATTACACAACTT+TGG | - | Chr1:72111128-72111147 | MsG0180004027.01.T01:CDS | 35.0% | |
| ! | ACCTTCAATCTGAAGTGTTA+AGG | - | Chr1:72112108-72112127 | MsG0180004027.01.T01:CDS | 35.0% |
| ! | CAATCTCTTTCAGCACTTTT+TGG | - | Chr1:72111204-72111223 | MsG0180004027.01.T01:intron | 35.0% |
| ! | TACCAAGCTTTGTATGTGAT+GGG | - | Chr1:72111597-72111616 | MsG0180004027.01.T01:intron | 35.0% |
| ! | TCTGGTTACTCTTCATCTTT+TGG | - | Chr1:72111813-72111832 | MsG0180004027.01.T01:CDS | 35.0% |
| ! | TTACCAAGCTTTGTATGTGA+TGG | - | Chr1:72111596-72111615 | MsG0180004027.01.T01:intron | 35.0% |
| !! | AATGGTGTTGTGAGTAATCA+TGG | - | Chr1:72111558-72111577 | MsG0180004027.01.T01:intron | 35.0% |
| !! | AGATACCTGTGATGCTATTT+TGG | + | Chr1:72111287-72111306 | None:intergenic | 35.0% |
| !! | CAGATTCTTTTCAAGACATG+TGG | - | Chr1:72111471-72111490 | MsG0180004027.01.T01:intron | 35.0% |
| !! | GCTTTTTCTGTGATGGAAAA+TGG | - | Chr1:72111540-72111559 | MsG0180004027.01.T01:intron | 35.0% |
| !!! | AGAAGAAAGAGGGGTTTTTT+TGG | + | Chr1:72111063-72111082 | None:intergenic | 35.0% |
| AATGGCCAAAATAGCATCAC+AGG | - | Chr1:72111279-72111298 | MsG0180004027.01.T01:intron | 40.0% | |
| AGGTTGTTACATGCTGTATG+AGG | - | Chr1:72111419-72111438 | MsG0180004027.01.T01:intron | 40.0% | |
| GAAGGAGCTCAATTTAGCAA+TGG | - | Chr1:72112150-72112169 | MsG0180004027.01.T01:CDS | 40.0% | |
| GCTATGACAGGACAAAATAC+AGG | - | Chr1:72112296-72112315 | MsG0180004027.01.T01:CDS | 40.0% | |
| GTTCTCTCAGACAAACTATG+TGG | - | Chr1:72111366-72111385 | MsG0180004027.01.T01:intron | 40.0% | |
| TATCCTAATGGGGTTCCAAA+GGG | - | Chr1:72111756-72111775 | MsG0180004027.01.T01:CDS | 40.0% | |
| TGATGAAGAAACGTGATGGT+AGG | - | Chr1:72112396-72112415 | MsG0180004027.01.T01:CDS | 40.0% | |
| TGGCTAAATGATGCTATGAC+AGG | - | Chr1:72112284-72112303 | MsG0180004027.01.T01:CDS | 40.0% | |
| TGTATGTGTCTGACATGTGT+TGG | + | Chr1:72112080-72112099 | None:intergenic | 40.0% | |
| TTATCCTAATGGGGTTCCAA+AGG | - | Chr1:72111755-72111774 | MsG0180004027.01.T01:CDS | 40.0% | |
| ! | CATAGTTTGTCTGAGAGAAC+TGG | + | Chr1:72111366-72111385 | None:intergenic | 40.0% |
| ! | CTTCAGATTGAAGGTGTGTT+TGG | + | Chr1:72112103-72112122 | None:intergenic | 40.0% |
| ! | GGTTACTCTTCATCTTTTGG+TGG | - | Chr1:72111816-72111835 | MsG0180004027.01.T01:CDS | 40.0% |
| !! | CAAAGAGCTCAAGTTGAATG+TGG | - | Chr1:72111678-72111697 | MsG0180004027.01.T01:CDS | 40.0% |
| !! | CTGGTTTTCCACAAGTTTCA+GGG | - | Chr1:72111445-72111464 | MsG0180004027.01.T01:intron | 40.0% |
| !! | GATGTTGGTGATTCTGATTG+TGG | - | Chr1:72111633-72111652 | MsG0180004027.01.T01:intron | 40.0% |
| !!! | AGACACTGCTTTTTCTGTGA+TGG | - | Chr1:72111533-72111552 | MsG0180004027.01.T01:intron | 40.0% |
| AAGCTGAACTCTTGCAGAGA+TGG | + | Chr1:72111397-72111416 | None:intergenic | 45.0% | |
| AAGGGTGGTCATTCTTCATC+TGG | - | Chr1:72111774-72111793 | MsG0180004027.01.T01:CDS | 45.0% | |
| ACAACAAATGCTGCTGGAAG+AGG | - | Chr1:72111495-72111514 | MsG0180004027.01.T01:intron | 45.0% | |
| ACAGGTATCTTCCAATGCAG+AGG | - | Chr1:72111297-72111316 | MsG0180004027.01.T01:intron | 45.0% | |
| ATCTGCATCCCTGAAACTTG+TGG | + | Chr1:72111456-72111475 | None:intergenic | 45.0% | |
| CAACAAATGCTGCTGGAAGA+GGG | - | Chr1:72111496-72111515 | MsG0180004027.01.T01:intron | 45.0% | |
| GACCCATCACATACAAAGCT+TGG | + | Chr1:72111602-72111621 | None:intergenic | 45.0% | |
| GATGAAGAATGACCACCCTT+TGG | + | Chr1:72111774-72111793 | None:intergenic | 45.0% | |
| TACATGCTGTATGAGGTTGC+TGG | - | Chr1:72111426-72111445 | MsG0180004027.01.T01:intron | 45.0% | |
| TCTGCAAGAGTTCAGCTTTC+AGG | - | Chr1:72111399-72111418 | MsG0180004027.01.T01:intron | 45.0% | |
| TGTGGAACAACAAATGCTGC+TGG | - | Chr1:72111489-72111508 | MsG0180004027.01.T01:intron | 45.0% | |
| TTGTGAGATCTCCTCTGCAT+TGG | + | Chr1:72111311-72111330 | None:intergenic | 45.0% | |
| ! | ATTAGGAGGTATAGCTGCTG+TGG | - | Chr1:72112337-72112356 | MsG0180004027.01.T01:CDS | 45.0% |
| ! | CAAGTTGACACTGATGCATG+TGG | + | Chr1:72112034-72112053 | None:intergenic | 45.0% |
| ! | GGTAGGTGTTGTGTTGTACA+TGG | - | Chr1:72112413-72112432 | MsG0180004027.01.T01:CDS | 45.0% |
| ! | TATAGCTGCTGTGGCGTTTT+TGG | - | Chr1:72112346-72112365 | MsG0180004027.01.T01:CDS | 45.0% |
| !! | GTCAACTTGTTGTGTTGCGT+AGG | - | Chr1:72112045-72112064 | MsG0180004027.01.T01:CDS | 45.0% |
| !! | TATGTGATGGGTCAGTGTGA+GGG | - | Chr1:72111609-72111628 | MsG0180004027.01.T01:intron | 45.0% |
| !!! | GCTGGTTTTCCACAAGTTTC+AGG | - | Chr1:72111444-72111463 | MsG0180004027.01.T01:intron | 45.0% |
| GGTCATTCTTCATCTGGTGG+TGG | - | Chr1:72111780-72111799 | MsG0180004027.01.T01:CDS | 50.0% | |
| GGTGGTCATTCTTCATCTGG+TGG | - | Chr1:72111777-72111796 | MsG0180004027.01.T01:CDS | 50.0% | |
| !! | GGTGGTGGATCATCTTCTTC+TGG | - | Chr1:72111795-72111814 | MsG0180004027.01.T01:CDS | 50.0% |
| !! | GTATGTGATGGGTCAGTGTG+AGG | - | Chr1:72111608-72111627 | MsG0180004027.01.T01:intron | 50.0% |
| CCTAATGGGGTTCCAAAGGG+TGG | - | Chr1:72111759-72111778 | MsG0180004027.01.T01:CDS | 55.0% | |
| GGTCAGTGTGAGGGTGATGT+TGG | - | Chr1:72111618-72111637 | MsG0180004027.01.T01:intron | 55.0% | |
| !! | CCACCCTTTGGAACCCCATT+AGG | + | Chr1:72111762-72111781 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 72111051 | 72112454 | 72111051 | ID=MsG0180004027.01;Name=MsG0180004027.01 |
| Chr1 | mRNA | 72111051 | 72112454 | 72111051 | ID=MsG0180004027.01.T01;Parent=MsG0180004027.01;Name=MsG0180004027.01.T01;_AED=0.30;_eAED=0.32;_QI=0|0|0|1|1|1|2|0|311 |
| Chr1 | exon | 72111668 | 72112454 | 72111668 | ID=MsG0180004027.01.T01:exon:5022;Parent=MsG0180004027.01.T01 |
| Chr1 | exon | 72111051 | 72111199 | 72111051 | ID=MsG0180004027.01.T01:exon:5021;Parent=MsG0180004027.01.T01 |
| Chr1 | CDS | 72111668 | 72112454 | 72111668 | ID=MsG0180004027.01.T01:cds;Parent=MsG0180004027.01.T01 |
| Chr1 | CDS | 72111051 | 72111199 | 72111051 | ID=MsG0180004027.01.T01:cds;Parent=MsG0180004027.01.T01 |
| Gene Sequence |
| Protein sequence |
