AlfalfaGEDB | MsG0480019051.01

M. sativa cultivar ZhongmuNo.1 / MsG0480019051.01

KEGG: K12834
GO: -
PFAM: PF03660

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019051.01.T01	KAG7019124.1	99.01	101	1	0	1	101	68	168	2.31E-66	207

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019051.01.T01	P0DI19	98.02	101	2	0	1	101	10	110	2.29E-67	200

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019051.01.T01	A0A445FPW2	99.010	101	1	0	1	101	78	178	1.67e-66	207

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005784.01	MsG0480019051.01	-0.821195	4.594984e-53	3.713271e-50
MsG0380014507.01	MsG0480019051.01	0.823262	1.522935e-53	1.304746e-50
MsG0480019051.01	MsG0480021279.01	0.817905	2.587170e-52	1.908330e-49
MsG0480019051.01	MsG0480022581.01	-0.821414	4.089289e-53	3.324624e-50
MsG0480019051.01	MsG0580024540.01	0.815565	8.661471e-52	5.992516e-49
MsG0480019051.01	MsG0580028107.01	0.810364	1.195996e-50	7.195155e-48
MsG0480019051.01	MsG0780041522.01	0.810483	1.127028e-50	6.801602e-48
MsG0480019051.01	MsG0880042896.01	0.827791	1.287316e-54	1.255495e-51
MsG0480019051.01	MsG0880046486.01	0.824740	6.851317e-54	6.122344e-51
MsG0380015697.01	MsG0480019051.01	-0.808032	3.779254e-50	2.137886e-47
MsG0380016760.01	MsG0480019051.01	0.805898	1.068985e-49	5.722733e-47
MsG0280007995.01	MsG0480019051.01	0.808520	2.975161e-50	1.704776e-47
MsG0280009166.01	MsG0480019051.01	0.812570	3.966741e-51	2.530922e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019051.01.T01	MTR_4g022900	100.000	101	0	0	1	101	10	110	2.06e-69	202
MsG0480019051.01.T01	MTR_3g464510	99.010	101	1	0	1	101	10	110	1.52e-68	201

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480019051.01.T01	AT2G30000	98.020	101	2	1	101	10	110	2.33e-68	200
MsG0480019051.01.T01	AT1G07170	98.020	101	2	1	101	10	110	2.33e-68	200
MsG0480019051.01.T01	AT1G07170	98.020	101	2	1	101	10	110	2.33e-68	200
MsG0480019051.01.T01	AT1G07170	98.020	101	2	1	101	10	110	2.33e-68	200
MsG0480019051.01.T01	AT1G07170	98.020	101	2	1	101	10	110	2.33e-68	200
MsG0480019051.01.T01	AT1G07170	98.020	101	2	1	101	10	110	2.33e-68	200
MsG0480019051.01.T01	AT1G07170	98.020	101	2	1	101	10	110	2.33e-68	200

Find 26 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTCCCTAAATTAACAATTT+TGG	0.085622	4:+13856072	None:intergenic
TGCCCCAAAATTGTTAATTT+AGG	0.174986	4:-13856076	MsG0480019051.01.T01:CDS
ATGCAACTACGGATCATTTC+AGG	0.240854	4:-13856185	MsG0480019051.01.T01:CDS
GCCCCAAAATTGTTAATTTA+GGG	0.268832	4:-13856075	MsG0480019051.01.T01:CDS
AATCGTCCAATGGCTATTCC+TGG	0.379932	4:+13856286	None:intergenic
TTCGTCCATGTACCCTCGTT+CGG	0.392439	4:-13856219	MsG0480019051.01.T01:CDS
GCACACAGCAGGAGAAAGAT+AGG	0.403394	4:-13856105	MsG0480019051.01.T01:CDS
TGGCTATTCCTGGGGTCTTA+CGG	0.411863	4:+13856296	None:intergenic
TCGTCCATGTACCCTCGTTC+GGG	0.438673	4:-13856218	MsG0480019051.01.T01:CDS
ATCGTCCAATGGCTATTCCT+GGG	0.496627	4:+13856287	None:intergenic
CAGGGTCGATGTGTTATATG+TGG	0.508838	4:-13856166	MsG0480019051.01.T01:CDS
TTCATAGAACAAATCAGTCT+TGG	0.519154	4:+13856048	None:intergenic
CAGACCCGAACGAGGGTACA+TGG	0.527375	4:+13856214	None:intergenic
ATCATGTGCCGTAAGACCCC+AGG	0.534925	4:-13856304	MsG0480019051.01.T01:CDS
TGCAACTACGGATCATTTCA+GGG	0.545593	4:-13856184	MsG0480019051.01.T01:CDS
TGTGTTATATGTGGAGGAGT+AGG	0.546814	4:-13856157	MsG0480019051.01.T01:CDS
CTGCAAGGAATGCACACAGC+AGG	0.557725	4:-13856116	MsG0480019051.01.T01:CDS
AAGACCCCAGGAATAGCCAT+TGG	0.573025	4:-13856292	MsG0480019051.01.T01:CDS
ATCTGATGCTTACTACTGCA+AGG	0.581899	4:-13856131	MsG0480019051.01.T01:CDS
GTCTGCGATGAATGCAACTA+CGG	0.590595	4:-13856196	MsG0480019051.01.T01:CDS
CACACAGCAGGAGAAAGATA+GGG	0.609822	4:-13856104	MsG0480019051.01.T01:CDS
TCGTCCAATGGCTATTCCTG+GGG	0.641471	4:+13856288	None:intergenic
GGTCGATGTGTTATATGTGG+AGG	0.641891	4:-13856163	MsG0480019051.01.T01:CDS
ATTCATCGCAGACCCGAACG+AGG	0.664455	4:+13856206	None:intergenic
CAGCAGGAGAAAGATAGGGA+TGG	0.685323	4:-13856100	MsG0480019051.01.T01:CDS
TTCATCGCAGACCCGAACGA+GGG	0.703508	4:+13856207	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ACTCCCTAAATTAACAATTT+TGG	+	Chr4:13856250-13856269	None:intergenic	25.0%
!!	CTCCCTAAATTAACAATTTT+GGG	+	Chr4:13856249-13856268	None:intergenic	25.0%
!!	TCCCTAAATTAACAATTTTG+GGG	+	Chr4:13856248-13856267	None:intergenic	25.0%
!!!	AAAAGTACGGTTTTAAGAAA+AGG	-	Chr4:13856298-13856317	MsG0480019051.01.T01:CDS	25.0%
	GCCCCAAAATTGTTAATTTA+GGG	-	Chr4:13856244-13856263	MsG0480019051.01.T01:CDS	30.0%
	TGCCCCAAAATTGTTAATTT+AGG	-	Chr4:13856243-13856262	MsG0480019051.01.T01:CDS	30.0%
	TTCATAGAACAAATCAGTCT+TGG	+	Chr4:13856274-13856293	None:intergenic	30.0%
	TTCTATGAACGCAAAAAGTA+CGG	-	Chr4:13856285-13856304	MsG0480019051.01.T01:CDS	30.0%
	CGATTGTGTGAAAAATGTGA+CGG	-	Chr4:13856051-13856070	MsG0480019051.01.T01:CDS	35.0%
!	TTTTTCACACAATCGTCCAA+TGG	+	Chr4:13856046-13856065	None:intergenic	35.0%
	ATCTGATGCTTACTACTGCA+AGG	-	Chr4:13856188-13856207	MsG0480019051.01.T01:CDS	40.0%
	ATGCAACTACGGATCATTTC+AGG	-	Chr4:13856134-13856153	MsG0480019051.01.T01:CDS	40.0%
	TGCAACTACGGATCATTTCA+GGG	-	Chr4:13856135-13856154	MsG0480019051.01.T01:CDS	40.0%
	TGTGTTATATGTGGAGGAGT+AGG	-	Chr4:13856162-13856181	MsG0480019051.01.T01:CDS	40.0%
	AATCGTCCAATGGCTATTCC+TGG	+	Chr4:13856036-13856055	None:intergenic	45.0%
	ATCGTCCAATGGCTATTCCT+GGG	+	Chr4:13856035-13856054	None:intergenic	45.0%
	CACACAGCAGGAGAAAGATA+GGG	-	Chr4:13856215-13856234	MsG0480019051.01.T01:CDS	45.0%
	CAGGGTCGATGTGTTATATG+TGG	-	Chr4:13856153-13856172	MsG0480019051.01.T01:CDS	45.0%
	GGTCGATGTGTTATATGTGG+AGG	-	Chr4:13856156-13856175	MsG0480019051.01.T01:CDS	45.0%
	GTCTGCGATGAATGCAACTA+CGG	-	Chr4:13856123-13856142	MsG0480019051.01.T01:CDS	45.0%
	AAGACCCCAGGAATAGCCAT+TGG	-	Chr4:13856027-13856046	MsG0480019051.01.T01:CDS	50.0%
	CAGCAGGAGAAAGATAGGGA+TGG	-	Chr4:13856219-13856238	MsG0480019051.01.T01:CDS	50.0%
	GCACACAGCAGGAGAAAGAT+AGG	-	Chr4:13856214-13856233	MsG0480019051.01.T01:CDS	50.0%
	TCGTCCAATGGCTATTCCTG+GGG	+	Chr4:13856034-13856053	None:intergenic	50.0%
	TGGCTATTCCTGGGGTCTTA+CGG	+	Chr4:13856026-13856045	None:intergenic	50.0%
	TTCGTCCATGTACCCTCGTT+CGG	-	Chr4:13856100-13856119	MsG0480019051.01.T01:CDS	50.0%
	ATTCATCGCAGACCCGAACG+AGG	+	Chr4:13856116-13856135	None:intergenic	55.0%
	CTGCAAGGAATGCACACAGC+AGG	-	Chr4:13856203-13856222	MsG0480019051.01.T01:CDS	55.0%
	TCGTCCATGTACCCTCGTTC+GGG	-	Chr4:13856101-13856120	MsG0480019051.01.T01:CDS	55.0%
	TTCATCGCAGACCCGAACGA+GGG	+	Chr4:13856115-13856134	None:intergenic	55.0%
!	CAGACCCGAACGAGGGTACA+TGG	+	Chr4:13856108-13856127	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	13856018	13856323	13856018	ID=MsG0480019051.01;Name=MsG0480019051.01
Chr4	mRNA	13856018	13856323	13856018	ID=MsG0480019051.01.T01;Parent=MsG0480019051.01;Name=MsG0480019051.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|101
Chr4	exon	13856018	13856323	13856018	ID=MsG0480019051.01.T01:exon:8592;Parent=MsG0480019051.01.T01
Chr4	CDS	13856018	13856323	13856018	ID=MsG0480019051.01.T01:cds;Parent=MsG0480019051.01.T01

Gene Sequence	>MsG0480019051.01.T01 ATGTGCCGTAAGACCCCAGGAATAGCCATTGGACGATTGTGTGAAAAATGTGACGGAAAGTGTGTGATATGCGACTCTTATGTTCGTCCATGTACCCTCGTTCGGGTCTGCGATGAATGCAACTACGGATCATTTCAGGGTCGATGTGTTATATGTGGAGGAGTAGGAATATCTGATGCTTACTACTGCAAGGAATGCACACAGCAGGAGAAAGATAGGGATGGTTGCCCCAAAATTGTTAATTTAGGGAGTGCCAAGACTGATTTGTTCTATGAACGCAAAAAGTACGGTTTTAAGAAAAGGTGA
Protein sequence	>MsG0480019051.01.T01 MCRKTPGIAIGRLCEKCDGKCVICDSYVRPCTLVRVCDECNYGSFQGRCVICGGVGISDAYYCKECTQQEKDRDGCPKIVNLGSAKTDLFYERKKYGFKKR*