Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016760.01.T01 | XP_003602483.1 | 96.591 | 176 | 4 | 1 | 1 | 176 | 138 | 311 | 7.48E-115 | 357 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048040.01 | MsG0380016760.01 | 0.842538 | 2.433128e-58 | 3.700895e-55 |
| MsG0080048922.01 | MsG0380016760.01 | 0.895519 | 8.871821e-76 | 9.237080e-72 |
| MsG0180000096.01 | MsG0380016760.01 | 0.903073 | 5.051833e-79 | 7.363267e-75 |
| MsG0180000118.01 | MsG0380016760.01 | 0.881916 | 1.612308e-70 | 9.623952e-67 |
| MsG0180001054.01 | MsG0380016760.01 | -0.803789 | 2.948128e-49 | 1.494939e-46 |
| MsG0180001169.01 | MsG0380016760.01 | 0.914545 | 1.691377e-84 | 4.226593e-80 |
| MsG0180001588.01 | MsG0380016760.01 | 0.800708 | 1.269780e-48 | 5.952769e-46 |
| MsG0180001829.01 | MsG0380016760.01 | 0.849332 | 3.460192e-60 | 6.537910e-57 |
| MsG0180001837.01 | MsG0380016760.01 | 0.824172 | 9.320200e-54 | 8.193178e-51 |
| MsG0180002738.01 | MsG0380016760.01 | 0.831585 | 1.535391e-55 | 1.673605e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016760.01.T01 | MTR_3g094850 | 96.591 | 176 | 4 | 1 | 1 | 176 | 138 | 311 | 9.06e-119 | 357 |
| MsG0380016760.01.T01 | MTR_3g094880 | 100.000 | 97 | 0 | 0 | 530 | 626 | 272 | 368 | 1.70e-61 | 209 |
| MsG0380016760.01.T01 | MTR_3g094880 | 53.968 | 126 | 22 | 2 | 430 | 519 | 14 | 139 | 8.94e-27 | 112 |
| MsG0380016760.01.T01 | MTR_3g094570 | 61.157 | 121 | 11 | 1 | 195 | 279 | 2 | 122 | 3.57e-35 | 133 |
| MsG0380016760.01.T01 | MTR_3g094570 | 97.727 | 44 | 1 | 0 | 280 | 323 | 184 | 227 | 4.29e-19 | 87.4 |
| MsG0380016760.01.T01 | MTR_3g094860 | 62.185 | 119 | 9 | 1 | 197 | 279 | 4 | 122 | 3.94e-35 | 133 |
| MsG0380016760.01.T01 | MTR_3g094860 | 97.727 | 44 | 1 | 0 | 280 | 323 | 184 | 227 | 4.42e-19 | 87.0 |
| MsG0380016760.01.T01 | MTR_3g094870 | 57.364 | 129 | 46 | 3 | 338 | 463 | 20 | 142 | 1.46e-34 | 129 |
| MsG0380016760.01.T01 | MTR_7g074060 | 65.217 | 115 | 31 | 5 | 330 | 441 | 68 | 176 | 1.50e-30 | 127 |
| MsG0380016760.01.T01 | MTR_5g098090 | 55.833 | 120 | 17 | 1 | 196 | 279 | 4 | 123 | 2.97e-30 | 119 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016760.01.T01 | AT2G21950 | 61.579 | 190 | 62 | 4 | 1 | 183 | 159 | 344 | 2.07e-71 | 235 |
| MsG0380016760.01.T01 | AT4G39520 | 90.816 | 98 | 9 | 0 | 529 | 626 | 272 | 369 | 2.27e-57 | 198 |
| MsG0380016760.01.T01 | AT4G39520 | 53.543 | 127 | 22 | 2 | 430 | 519 | 14 | 140 | 1.29e-26 | 112 |
| MsG0380016760.01.T01 | AT4G39550 | 47.531 | 162 | 77 | 7 | 1 | 159 | 180 | 336 | 7.94e-34 | 134 |
| MsG0380016760.01.T01 | AT1G74060 | 56.303 | 119 | 16 | 1 | 197 | 279 | 4 | 122 | 1.67e-30 | 120 |
| MsG0380016760.01.T01 | AT1G74060 | 75.610 | 41 | 10 | 0 | 283 | 323 | 187 | 227 | 3.88e-12 | 67.0 |
| MsG0380016760.01.T01 | AT1G74050 | 55.462 | 119 | 17 | 1 | 197 | 279 | 4 | 122 | 6.81e-30 | 118 |
| MsG0380016760.01.T01 | AT1G74050 | 75.610 | 41 | 10 | 0 | 283 | 323 | 187 | 227 | 3.64e-12 | 67.0 |
| MsG0380016760.01.T01 | AT1G18540 | 57.018 | 114 | 13 | 1 | 202 | 279 | 9 | 122 | 1.84e-29 | 117 |
| MsG0380016760.01.T01 | AT1G72660 | 50.505 | 99 | 47 | 1 | 529 | 625 | 189 | 287 | 1.55e-28 | 117 |
Find 215 sgRNAs with CRISPR-Local
Find 1335 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACACAAGCCTCAGAGAGT+CGG | 0.626180 | 3:+87510547 | MsG0380016760.01.T01:CDS |
| AAATAAGAAGGGGAAAGAAA+TGG | 0.497591 | 3:+87491024 | MsG0380016760.01.T01:CDS |
| AAGACTTTCCCCAGGGTTAT+TGG | 0.227823 | 3:+87510845 | MsG0380016760.01.T01:three_prime_UTR |
| AAGGCTACCGCTCATCATTT+GGG | 0.320743 | 3:+87504084 | MsG0380016760.01.T01:CDS |
| AAGGGCAGGAGTATGGTTTC+TGG | 0.291085 | 3:+87500425 | MsG0380016760.01.T01:CDS |
| AATGAAATTACGCATCAGTT+TGG | 0.405270 | 3:+87511130 | MsG0380016760.01.T01:three_prime_UTR |
| AATGATGCAGTGCTCTGGTC+TGG | 0.288648 | 3:+87510512 | MsG0380016760.01.T01:intron |
| AATGGAGCGTGGGAGAGCGT+TGG | 0.540307 | 3:+87490776 | MsG0380016760.01.T01:CDS |
| AATGGCTGCAGGTGTCATGT+TGG | 0.659576 | 3:+87500378 | MsG0380016760.01.T01:CDS |
| AATTTGAATGCAAATAAGAA+GGG | 0.517396 | 3:+87491013 | MsG0380016760.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAAAATCAACATCTGACG+CGG | - | Chr3:87494351-87494370 | None:intergenic | 30.0% | |
| AAAAATTTGTCAAGTGCAGT+AGG | - | Chr3:87510463-87510482 | None:intergenic | 30.0% | |
| AAAATGTTAACTAGTGTGTC+TGG | + | Chr3:87502353-87502372 | MsG0380016760.01.T01:intron | 30.0% | |
| AAACATTAGAGAGATAGTTG+GGG | + | Chr3:87508035-87508054 | MsG0380016760.01.T01:intron | 30.0% | |
| AAACTTTAAACATAGGGAGA+GGG | - | Chr3:87504489-87504508 | None:intergenic | 30.0% | |
| AAATAAAGTGATTGTGTACG+AGG | + | Chr3:87505136-87505155 | MsG0380016760.01.T01:intron | 30.0% | |
| AAATAAGAAGGGGAAAGAAA+TGG | + | Chr3:87491024-87491043 | MsG0380016760.01.T01:CDS | 30.0% | |
| AAATCCGTTGTTTGTGAATT+GGG | + | Chr3:87491924-87491943 | MsG0380016760.01.T01:intron | 30.0% | |
| AAATGTTAACTAGTGTGTCT+GGG | + | Chr3:87502354-87502373 | MsG0380016760.01.T01:intron | 30.0% | |
| AACAACAACAACAAAAACAC+TGG | - | Chr3:87504185-87504204 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 87490527 | 87511153 | 87490527 | ID=MsG0380016760.01;Name=MsG0380016760.01 |
| Chr3 | mRNA | 87490527 | 87511153 | 87490527 | ID=MsG0380016760.01.T01;Parent=MsG0380016760.01;Name=MsG0380016760.01.T01;_AED=0.50;_eAED=0.53;_QI=0|0.23|0.35|1|1|1|14|391|626 |
| Chr3 | exon | 87490527 | 87491053 | 87490527 | ID=MsG0380016760.01.T01:exon:22560;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87495060 | 87495260 | 87495060 | ID=MsG0380016760.01.T01:exon:22561;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87496079 | 87496185 | 87496079 | ID=MsG0380016760.01.T01:exon:22562;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87498868 | 87499034 | 87498868 | ID=MsG0380016760.01.T01:exon:22563;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87500244 | 87500454 | 87500244 | ID=MsG0380016760.01.T01:exon:22564;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87500872 | 87500948 | 87500872 | ID=MsG0380016760.01.T01:exon:22565;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87504063 | 87504124 | 87504063 | ID=MsG0380016760.01.T01:exon:22566;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87504328 | 87504385 | 87504328 | ID=MsG0380016760.01.T01:exon:22567;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87505931 | 87506002 | 87505931 | ID=MsG0380016760.01.T01:exon:22568;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87506914 | 87506973 | 87506914 | ID=MsG0380016760.01.T01:exon:22569;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87509101 | 87509144 | 87509101 | ID=MsG0380016760.01.T01:exon:22570;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87509799 | 87509990 | 87509799 | ID=MsG0380016760.01.T01:exon:22571;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87510522 | 87510573 | 87510522 | ID=MsG0380016760.01.T01:exon:22572;Parent=MsG0380016760.01.T01 |
| Chr3 | exon | 87510712 | 87511153 | 87510712 | ID=MsG0380016760.01.T01:exon:22573;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87490527 | 87491053 | 87490527 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87495060 | 87495260 | 87495060 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87496079 | 87496185 | 87496079 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87498868 | 87499034 | 87498868 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87500244 | 87500454 | 87500244 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87500872 | 87500948 | 87500872 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87504063 | 87504124 | 87504063 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87504328 | 87504385 | 87504328 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87505931 | 87506002 | 87505931 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87506914 | 87506973 | 87506914 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87509101 | 87509144 | 87509101 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87509799 | 87509990 | 87509799 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87510522 | 87510573 | 87510522 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | CDS | 87510712 | 87510762 | 87510712 | ID=MsG0380016760.01.T01:cds;Parent=MsG0380016760.01.T01 |
| Chr3 | three_prime_UTR | 87510763 | 87511153 | 87510763 | ID=MsG0380016760.01.T01:three_prime_utr;Parent=MsG0380016760.01.T01 |
| Gene Sequence |
>MsG0380016760.01.T01 ATGCGGGTGGCGCGTGAATTCGCAGCTGCTGGTGTTATCGACGGGAAAATCTACGTCATCGGTGGTTGCGTGCCGGATAATTTTTCCAGATCAGCAAATTGGTCGGAGGTTTTCGATCCTGTTACCAACCGCTGGGAAAGTGTTCCTAGTCCACCAGAAATTCGTGAGAAATGGATGCACGCTAGCGCGGTCGTAGACGGCAAAGTTTACGCGATGGCGGATCGCGGTGGTGTTTTGTTTGATCCTTATAATGGAGCGTGGGAGAGCGTTGGGAGGGAGCTTGATTTAGGTTGGCGCGGGAGAGCAACTGTTGTGGATGGGATTTTGTATTGCTATGATTATTTAGGGAAAATTAAGGGTTTTGATGTGAAGAAAGGGTTGTGGAAGGAGTTGAAAGGTTTGGATAAGAGTTTACCAAGGTTTCTGTGTGGGGCTACAATGGCTGATGTTGGTGGGAAATTGGTTGTGGTTTGGGAGTGTCAAAGGAATTTGAATGCAAATAAGAAGGGGAAAGAAATGGAGATTTGGTTTAGACAGGGAGGGAGGCAAAGAACAGCACCATCCCATCACACCAATAGCAATGGCACCAAAAACAAGAACCGCAGAGTGAGCCGTAACCCTGACTTGATCAGGGGTATCGGAAAATACTCGAGATCTGCTATGTATCACAAACGTGGTCTCTGGGCAATCAAGGCTAAGCACGGTGGAGCTTTCCTCGCCACGATCCCGGCTTCTATTACTCCAGGGACAGTGTTGATTCTTCTTGCTGGAAGATTTAAGGGAAAGAGAGTTGTGTTTCTCAAACAGCTTCCTTCTGGACTTCTTCTTGTTACCGGACAAAAGGTGCTCCCTCAGGAGAAGAAAGATGATCAAAAGACTGTCGATGCTGGTTTGTTAAAAGCCATTGACAGTGTTCCAGACTTGAAAACTTACCTAGGTGCCAGGTTTTCCTTGAAGGCAGGTGTGAAGCACATGAGTTGGTCTTCTAAAGGAAATAAGAGTGAAGAGTGGAGCAGTAGAGTTTCGGTTTCAACGGTTGAGTTGTTTACGTTGTCGATGGCAGCTGCTACTGGTTTAGGTGCAGTGCCGTTCTTCTTTTTAGAGCTTGATCCGCAATGGGTTGGGTTGTGTAACGGAATGGCTGCAGGTGTCATGTTGGCTGCAAGTTTTGATTTGATACAGGAAGGGCAGGAGTATGGTTTCTGGAAGCTGGGCTATTTTAGATTTATAGTATTCTCACTTTTGTCTTGCATGTCTCTTGGATTTCAGTTTCTTGAGCGATATGGAGAAATGGCGAAAACTCAAAAGAACAAGGCTACCGCTCATCATTTGGGGTTACTGAAGGTAAATATATTGGATAGTGTTTTAGGGATTGCGTTGGAAGTGCAATTCTGCTCAGAAGTTTTATTTGTTGCTTCCTATGAGTTCACTACACTAACTTGCATTCCAGGTGTGATCACATATCGTGGAGCAAAAATTCAGTTGCTGGATCTCCCTGGAATTATTGAGGGTGCCAAGGATGGAAAGGGAAGAGGAAGGCAGGTTAAACAAAGAGCCACCAAACCTGACTTTCAGGGAAAGAAAGGTGCACACCTGGAGTGGAACCTTGATGGGCTTCTGGATAAAGTTTGGGAGTATCTTAATTTAACTCGTATTTATACTAAACCTAAAGGTATGAATCCTGACTACGAAGACCCTGTAATATTGTCATCTAAGAAGAAGACAGTTGAAGATTTCTGTGATCGCATTCACAAGGACATGCTTAAACAGTTTAAGTATGCTCTGGTCTGGGGTTCAAGTGCAAAACACAAGCCTCAGAGAGTCGGCAAGGAACATGAACTTGAAGACGAAGACGTTGTTCAGATCATCAAGAAAGTATGA |
| Protein sequence |
>MsG0380016760.01.T01 MRVAREFAAAGVIDGKIYVIGGCVPDNFSRSANWSEVFDPVTNRWESVPSPPEIREKWMHASAVVDGKVYAMADRGGVLFDPYNGAWESVGRELDLGWRGRATVVDGILYCYDYLGKIKGFDVKKGLWKELKGLDKSLPRFLCGATMADVGGKLVVVWECQRNLNANKKGKEMEIWFRQGGRQRTAPSHHTNSNGTKNKNRRVSRNPDLIRGIGKYSRSAMYHKRGLWAIKAKHGGAFLATIPASITPGTVLILLAGRFKGKRVVFLKQLPSGLLLVTGQKVLPQEKKDDQKTVDAGLLKAIDSVPDLKTYLGARFSLKAGVKHMSWSSKGNKSEEWSSRVSVSTVELFTLSMAAATGLGAVPFFFLELDPQWVGLCNGMAAGVMLAASFDLIQEGQEYGFWKLGYFRFIVFSLLSCMSLGFQFLERYGEMAKTQKNKATAHHLGLLKVNILDSVLGIALEVQFCSEVLFVASYEFTTLTCIPGVITYRGAKIQLLDLPGIIEGAKDGKGRGRQVKQRATKPDFQGKKGAHLEWNLDGLLDKVWEYLNLTRIYTKPKGMNPDYEDPVILSSKKKTVEDFCDRIHKDMLKQFKYALVWGSSAKHKPQRVGKEHELEDEDVVQIIKKV* |
