AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046486.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046486.01.T01 MTR_4g109130 100.000 160 0 0 1 160 1 160 3.73e-118 330
MsG0880046486.01.T01 MTR_3g437680 96.250 160 6 0 1 160 1 160 7.28e-113 317
MsG0880046486.01.T01 MTR_3g437680 96.250 160 6 0 1 160 1 160 7.28e-113 317
MsG0880046486.01.T01 MTR_4g130917 93.711 159 10 0 1 159 1 159 3.59e-109 308
MsG0880046486.01.T01 MTR_5g042990 91.824 159 13 0 1 159 1 159 1.19e-107 304
MsG0880046486.01.T01 MTR_4g130917 92.913 127 9 0 1 127 1 127 5.95e-86 248
MsG0880046486.01.T01 MTR_5g042990 92.126 127 10 0 1 127 1 127 1.42e-85 247
MsG0880046486.01.T01 MTR_5g042990 86.232 138 19 0 1 138 1 138 2.55e-85 247
MsG0880046486.01.T01 MTR_4g109130 90.000 120 12 0 1 120 1 120 2.89e-75 221
MsG0880046486.01.T01 MTR_4g112440 71.569 102 19 1 1 102 1 92 6.52e-48 150
MsG0880046486.01.T01 MTR_4g130907 46.094 128 21 2 18 145 42 121 1.62e-25 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046486.01.T01 AT1G13950 91.195 159 13 1 1 159 1 158 7.23e-104 294
MsG0880046486.01.T01 AT1G69410 86.792 159 20 1 1 159 1 158 5.64e-101 287
MsG0880046486.01.T01 AT1G26630 81.646 158 28 1 1 158 1 157 3.47e-95 272
MsG0880046486.01.T01 AT1G26630 83.465 127 20 1 1 127 1 126 4.40e-76 223

Find 57 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGGTGTTGGTGGTGTTTA+TGG 0.113621 8:-73944770 MsG0880046486.01.T01:three_prime_UTR
TCAAACGAGTATTTCCTTAT+TGG 0.168992 8:-73944698 MsG0880046486.01.T01:three_prime_UTR
CAAACGAGTATTTCCTTATT+GGG 0.252830 8:-73944697 MsG0880046486.01.T01:three_prime_UTR
TTCGTCAGACATATTGATTC+AGG 0.282730 8:+73946287 None:intergenic
CATCGGCCTTAGATTCAAAT+TGG 0.297056 8:+73946259 None:intergenic
CTCATTGACATTTCTGAAGA+TGG 0.299146 8:-73945770 MsG0880046486.01.T01:CDS
GTGAGCTTGCTCACTGAAAA+TGG 0.342511 8:-73945172 MsG0880046486.01.T01:CDS
TAAAGGACAAATACCCAATA+AGG 0.344877 8:+73944684 None:intergenic
CTTGGCATGTCCATGCTTCC+CGG 0.359341 8:+73946020 None:intergenic
ATCTGTGCTTTGAAGGATAT+TGG 0.363464 8:-73944903 MsG0880046486.01.T01:CDS
AGAGCACCAATTTGAATCTA+AGG 0.377994 8:-73946265 MsG0880046486.01.T01:CDS
AGCTTGTTGAGGATAAGTTT+TGG 0.388597 8:+73946227 None:intergenic
TACTATACTTGTAAAGATAA+AGG 0.392946 8:+73944667 None:intergenic
ATGAGAACAGAGATTTGTTT+CGG 0.409267 8:+73946339 None:intergenic
CGTAAAACTCTGCTCTAGTC+TGG 0.417524 8:+73944594 None:intergenic
CTGTTCTCATTATCGCTCTT+CGG 0.419231 8:-73946326 MsG0880046486.01.T01:five_prime_UTR
TAACTATGTATCCATTCTTA+CGG 0.463557 8:+73946196 None:intergenic
GGAGCAGATCTGTGCTTTGA+AGG 0.467522 8:-73944910 MsG0880046486.01.T01:CDS
AATGTCAATGAGCTGATAAT+CGG 0.473877 8:+73945781 None:intergenic
GCTGGTACCATCCGTAAGAA+TGG 0.484451 8:-73946207 MsG0880046486.01.T01:CDS
TGATTCAGGAGCGGCGGTGG+CGG 0.485987 8:+73946301 None:intergenic
TGTATCCGTGATGTCTGCTA+TGG 0.510337 8:-73944937 MsG0880046486.01.T01:CDS
CTTTGTGTGCAGATCAAGGA+TGG 0.512763 8:-73944984 MsG0880046486.01.T01:intron
CACTGAAAATGGAAACACTA+AGG 0.519818 8:-73945161 MsG0880046486.01.T01:CDS
TGTGAGACTAGTGGTGTTGG+TGG 0.526439 8:-73944779 MsG0880046486.01.T01:three_prime_UTR
GAAGTTTCGACTTCGAAGAC+CGG 0.527896 8:-73946039 MsG0880046486.01.T01:CDS
TATTGATTCAGGAGCGGCGG+TGG 0.530564 8:+73946298 None:intergenic
GAATAATCACCTTGCATGGA+CGG 0.531922 8:+73946166 None:intergenic
GGATGGATTTGCTGAGGGAA+AGG 0.534900 8:-73944967 MsG0880046486.01.T01:CDS
TGTCATCGGTGGGAAGTTTG+AGG 0.539905 8:+73945134 None:intergenic
ACTTCGAAGACCGGGAAGCA+TGG 0.555740 8:-73946030 MsG0880046486.01.T01:CDS
CTGAGTGAGCAGACTGTCAT+CGG 0.555770 8:+73945120 None:intergenic
TTTGAATCTAAGGCCGATGC+CGG 0.559985 8:-73946255 MsG0880046486.01.T01:CDS
TTACGAATAATCACCTTGCA+TGG 0.560163 8:+73946162 None:intergenic
TCTTGTGAGACTAGTGGTGT+TGG 0.565302 8:-73944782 MsG0880046486.01.T01:three_prime_UTR
CTTCACTTCAAAGAAGCTTG+AGG 0.580602 8:-73945974 MsG0880046486.01.T01:CDS
AAAACTTATCCTCAACAAGC+TGG 0.581192 8:-73946225 MsG0880046486.01.T01:CDS
AGTGAGCAGACTGTCATCGG+TGG 0.592770 8:+73945123 None:intergenic
AAGTTTCGACTTCGAAGACC+GGG 0.594274 8:-73946038 MsG0880046486.01.T01:CDS
TGTGCTTTGAAGGATATTGG+TGG 0.594489 8:-73944900 MsG0880046486.01.T01:CDS
CGGATGGTACCAGCTTGTTG+AGG 0.595998 8:+73946216 None:intergenic
CGTGATGTCTGCTATGGGTG+AGG 0.597852 8:-73944931 MsG0880046486.01.T01:CDS
CGATGACAGTCTGCTCACTC+AGG 0.597987 8:-73945119 MsG0880046486.01.T01:intron
ATAGATTCTTGTGAGACTAG+TGG 0.603554 8:-73944788 MsG0880046486.01.T01:three_prime_UTR
TATGTATCCATTCTTACGGA+TGG 0.607030 8:+73946200 None:intergenic
ACATCACAATTATGTGAAGA+CGG 0.610965 8:+73945943 None:intergenic
GTATCCGTGATGTCTGCTAT+GGG 0.612684 8:-73944936 MsG0880046486.01.T01:CDS
GTGAGCAGACTGTCATCGGT+GGG 0.615967 8:+73945124 None:intergenic
GATCAAGGATGGATTTGCTG+AGG 0.618132 8:-73944973 MsG0880046486.01.T01:CDS
ATCAAGGATGGATTTGCTGA+GGG 0.621184 8:-73944972 MsG0880046486.01.T01:CDS
CAATTGAAAGTGAAGACAAG+TGG 0.623911 8:+73946367 None:intergenic
AATAGCAACAAAATGACACT+TGG 0.628478 8:+73946002 None:intergenic
CAGACATCACGGATACAACA+AGG 0.636222 8:+73944943 None:intergenic
ACATATTGATTCAGGAGCGG+CGG 0.647198 8:+73946295 None:intergenic
CTCACCCATAGCAGACATCA+CGG 0.653023 8:+73944932 None:intergenic
CAGACATATTGATTCAGGAG+CGG 0.719014 8:+73946292 None:intergenic
TAATCGGTACGATTCACGTG+AGG 0.748646 8:+73945797 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAACAAACTAAATTAAAA+CGG - Chr8:73945584-73945603 MsG0880046486.01.T01:intron 10.0%
!! TAAACAAACTAAATTAAAAC+GGG - Chr8:73945585-73945604 MsG0880046486.01.T01:intron 15.0%
!!! CAATTGTTTTTATTTTTGTT+AGG - Chr8:73945101-73945120 MsG0880046486.01.T01:intron 15.0%
!! TACTATACTTGTAAAGATAA+AGG + Chr8:73946259-73946278 None:intergenic 20.0%
!! TTCATAATATAGCTATCAAA+AGG + Chr8:73945673-73945692 None:intergenic 20.0%
!!! ACAAAATAATCACTTTTCAA+AGG + Chr8:73945207-73945226 None:intergenic 20.0%
!!! TGATTATGTTTTTGTATTGT+AGG - Chr8:73945729-73945748 MsG0880046486.01.T01:intron 20.0%
!!! TTTAGTTTGTTTAAGCTTTA+TGG + Chr8:73945578-73945597 None:intergenic 20.0%
! AGAGCTTAGAAAAAAGATTT+CGG + Chr8:73945548-73945567 None:intergenic 25.0%
! TAACTATGTATCCATTCTTA+CGG + Chr8:73944730-73944749 None:intergenic 25.0%
! TAATCTGATTTAGATTAGTG+TGG + Chr8:73945488-73945507 None:intergenic 25.0%
!! TTTTTGTACTTCGTTATTCA+TGG - Chr8:73944812-73944831 MsG0880046486.01.T01:three_prime_UTR 25.0%
!!! TGTTTTTTGTTGTTGCTATT+AGG - Chr8:73944856-73944875 MsG0880046486.01.T01:three_prime_UTR 25.0%
AATAGCAACAAAATGACACT+TGG + Chr8:73944924-73944943 None:intergenic 30.0%
AATGTCAATGAGCTGATAAT+CGG + Chr8:73945145-73945164 None:intergenic 30.0%
ACATCACAATTATGTGAAGA+CGG + Chr8:73944983-73945002 None:intergenic 30.0%
ATAAGCCTGTTACAGATTAA+TGG + Chr8:73945645-73945664 None:intergenic 30.0%
CAAACGAGTATTTCCTTATT+GGG - Chr8:73946226-73946245 MsG0880046486.01.T01:CDS 30.0%
GAGCTTAGAAAAAAGATTTC+GGG + Chr8:73945547-73945566 None:intergenic 30.0%
TAAAGGACAAATACCCAATA+AGG + Chr8:73946242-73946261 None:intergenic 30.0%
TAAGCCTGTTACAGATTAAT+GGG + Chr8:73945644-73945663 None:intergenic 30.0%
TCAAACGAGTATTTCCTTAT+TGG - Chr8:73946225-73946244 MsG0880046486.01.T01:CDS 30.0%
! ATGAGAACAGAGATTTGTTT+CGG + Chr8:73944587-73944606 None:intergenic 30.0%
! GATTTGTTCTTGCATTGTAA+TGG - Chr8:73945835-73945854 MsG0880046486.01.T01:intron 30.0%
! GGTACAATAACTTTTGTAAC+AGG + Chr8:73945622-73945641 None:intergenic 30.0%
! TGGTGTTTATGGATTTTTGT+TGG - Chr8:73946164-73946183 MsG0880046486.01.T01:intron 30.0%
AAAACTTATCCTCAACAAGC+TGG - Chr8:73944698-73944717 MsG0880046486.01.T01:three_prime_UTR 35.0%
AAGCCTGTTACAGATTAATG+GGG + Chr8:73945643-73945662 None:intergenic 35.0%
ATAGATTCTTGTGAGACTAG+TGG - Chr8:73946135-73946154 MsG0880046486.01.T01:intron 35.0%
CACTGAAAATGGAAACACTA+AGG - Chr8:73945762-73945781 MsG0880046486.01.T01:intron 35.0%
CATACACAAAATCAACTCAC+CGG + Chr8:73945439-73945458 None:intergenic 35.0%
CCATACCCTTCAACATAAAA+GGG + Chr8:73945416-73945435 None:intergenic 35.0%
TATGTATCCATTCTTACGGA+TGG + Chr8:73944726-73944745 None:intergenic 35.0%
TATTAAAAACCGTCCATGCA+AGG - Chr8:73944748-73944767 MsG0880046486.01.T01:three_prime_UTR 35.0%
TTACGAATAATCACCTTGCA+TGG + Chr8:73944764-73944783 None:intergenic 35.0%
! AGAGCACCAATTTGAATCTA+AGG - Chr8:73944658-73944677 MsG0880046486.01.T01:three_prime_UTR 35.0%
! CTCATTGACATTTCTGAAGA+TGG - Chr8:73945153-73945172 MsG0880046486.01.T01:CDS 35.0%
! TCAGACCCTTTTATGTTGAA+GGG - Chr8:73945408-73945427 MsG0880046486.01.T01:intron 35.0%
! TTCAGACCCTTTTATGTTGA+AGG - Chr8:73945407-73945426 MsG0880046486.01.T01:intron 35.0%
!! ATCTGTGCTTTGAAGGATAT+TGG - Chr8:73946020-73946039 MsG0880046486.01.T01:CDS 35.0%
!! TTCGTCAGACATATTGATTC+AGG + Chr8:73944639-73944658 None:intergenic 35.0%
!!! AGCTTGTTGAGGATAAGTTT+TGG + Chr8:73944699-73944718 None:intergenic 35.0%
CATCGGCCTTAGATTCAAAT+TGG + Chr8:73944667-73944686 None:intergenic 40.0%
CTGTTCTCATTATCGCTCTT+CGG - Chr8:73944597-73944616 MsG0880046486.01.T01:three_prime_UTR 40.0%
CTTCACTTCAAAGAAGCTTG+AGG - Chr8:73944949-73944968 MsG0880046486.01.T01:CDS 40.0%
GAATAATCACCTTGCATGGA+CGG + Chr8:73944760-73944779 None:intergenic 40.0%
GCCATACCCTTCAACATAAA+AGG + Chr8:73945417-73945436 None:intergenic 40.0%
GTACCCCATTAATCTGTAAC+AGG - Chr8:73945637-73945656 MsG0880046486.01.T01:intron 40.0%
TTTGCTTTGTGTGCAGATCA+AGG - Chr8:73945935-73945954 MsG0880046486.01.T01:intron 40.0%
! CCCTTTTATGTTGAAGGGTA+TGG - Chr8:73945413-73945432 MsG0880046486.01.T01:intron 40.0%
! TTTATGTTGAAGGGTATGGC+CGG - Chr8:73945417-73945436 MsG0880046486.01.T01:intron 40.0%
!! ATCAAGGATGGATTTGCTGA+GGG - Chr8:73945951-73945970 MsG0880046486.01.T01:CDS 40.0%
!! CAGACATATTGATTCAGGAG+CGG + Chr8:73944634-73944653 None:intergenic 40.0%
!! TGTGCTTTGAAGGATATTGG+TGG - Chr8:73946023-73946042 MsG0880046486.01.T01:CDS 40.0%
!!! AGGATAAGTTTTGGAAGCAC+CGG + Chr8:73944690-73944709 None:intergenic 40.0%
AAGTTTCGACTTCGAAGACC+GGG - Chr8:73944885-73944904 MsG0880046486.01.T01:exon 45.0%
CAGACATCACGGATACAACA+AGG + Chr8:73945983-73946002 None:intergenic 45.0%
CGTAAAACTCTGCTCTAGTC+TGG + Chr8:73946332-73946351 None:intergenic 45.0%
CTTTGTGTGCAGATCAAGGA+TGG - Chr8:73945939-73945958 MsG0880046486.01.T01:intron 45.0%
GAAGTTTCGACTTCGAAGAC+CGG - Chr8:73944884-73944903 MsG0880046486.01.T01:exon 45.0%
GTATCCGTGATGTCTGCTAT+GGG - Chr8:73945987-73946006 MsG0880046486.01.T01:CDS 45.0%
TAATCGGTACGATTCACGTG+AGG + Chr8:73945129-73945148 None:intergenic 45.0%
TCTTGTGAGACTAGTGGTGT+TGG - Chr8:73946141-73946160 MsG0880046486.01.T01:intron 45.0%
TGTATCCGTGATGTCTGCTA+TGG - Chr8:73945986-73946005 MsG0880046486.01.T01:CDS 45.0%
! GTGAGCTTGCTCACTGAAAA+TGG - Chr8:73945751-73945770 MsG0880046486.01.T01:intron 45.0%
! TTTGAATCTAAGGCCGATGC+CGG - Chr8:73944668-73944687 MsG0880046486.01.T01:three_prime_UTR 45.0%
!! ACATATTGATTCAGGAGCGG+CGG + Chr8:73944631-73944650 None:intergenic 45.0%
!! AGTGGTGTTGGTGGTGTTTA+TGG - Chr8:73946153-73946172 MsG0880046486.01.T01:intron 45.0%
!! GATCAAGGATGGATTTGCTG+AGG - Chr8:73945950-73945969 MsG0880046486.01.T01:CDS 45.0%
CTCACCCATAGCAGACATCA+CGG + Chr8:73945994-73946013 None:intergenic 50.0%
CTGAGTGAGCAGACTGTCAT+CGG + Chr8:73945806-73945825 None:intergenic 50.0%
GCTGGTACCATCCGTAAGAA+TGG - Chr8:73944716-73944735 MsG0880046486.01.T01:three_prime_UTR 50.0%
GGAGCAGATCTGTGCTTTGA+AGG - Chr8:73946013-73946032 MsG0880046486.01.T01:CDS 50.0%
TGTCATCGGTGGGAAGTTTG+AGG + Chr8:73945792-73945811 None:intergenic 50.0%
! TGTGAGACTAGTGGTGTTGG+TGG - Chr8:73946144-73946163 MsG0880046486.01.T01:intron 50.0%
!! GGATGGATTTGCTGAGGGAA+AGG - Chr8:73945956-73945975 MsG0880046486.01.T01:CDS 50.0%
!!! AGTTTTGGAAGCACCGGCAT+CGG + Chr8:73944684-73944703 None:intergenic 50.0%
ACTTCGAAGACCGGGAAGCA+TGG - Chr8:73944893-73944912 MsG0880046486.01.T01:CDS 55.0%
AGTGAGCAGACTGTCATCGG+TGG + Chr8:73945803-73945822 None:intergenic 55.0%
CGATGACAGTCTGCTCACTC+AGG - Chr8:73945804-73945823 MsG0880046486.01.T01:intron 55.0%
CGTGATGTCTGCTATGGGTG+AGG - Chr8:73945992-73946011 MsG0880046486.01.T01:CDS 55.0%
CTTGGCATGTCCATGCTTCC+CGG + Chr8:73944906-73944925 None:intergenic 55.0%
GTGAGCAGACTGTCATCGGT+GGG + Chr8:73945802-73945821 None:intergenic 55.0%
!! CGGATGGTACCAGCTTGTTG+AGG + Chr8:73944710-73944729 None:intergenic 55.0%
!! TATTGATTCAGGAGCGGCGG+TGG + Chr8:73944628-73944647 None:intergenic 55.0%
TGATTCAGGAGCGGCGGTGG+CGG + Chr8:73944625-73944644 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 73944558 73946387 73944558 ID=MsG0880046486.01;Name=MsG0880046486.01
Chr8 mRNA 73944558 73946387 73944558 ID=MsG0880046486.01.T01;Parent=MsG0880046486.01;Name=MsG0880046486.01.T01;_AED=0.48;_eAED=0.48;_QI=89|1|1|1|1|1|5|332|160
Chr8 exon 73944558 73944994 73944558 ID=MsG0880046486.01.T01:exon:19021;Parent=MsG0880046486.01.T01
Chr8 exon 73945120 73945194 73945120 ID=MsG0880046486.01.T01:exon:19020;Parent=MsG0880046486.01.T01
Chr8 exon 73945766 73945822 73945766 ID=MsG0880046486.01.T01:exon:19019;Parent=MsG0880046486.01.T01
Chr8 exon 73945945 73946067 73945945 ID=MsG0880046486.01.T01:exon:19018;Parent=MsG0880046486.01.T01
Chr8 exon 73946176 73946387 73946176 ID=MsG0880046486.01.T01:exon:19017;Parent=MsG0880046486.01.T01
Chr8 five_prime_UTR 73946299 73946387 73946299 ID=MsG0880046486.01.T01:five_prime_utr;Parent=MsG0880046486.01.T01
Chr8 CDS 73946176 73946298 73946176 ID=MsG0880046486.01.T01:cds;Parent=MsG0880046486.01.T01
Chr8 CDS 73945945 73946067 73945945 ID=MsG0880046486.01.T01:cds;Parent=MsG0880046486.01.T01
Chr8 CDS 73945766 73945822 73945766 ID=MsG0880046486.01.T01:cds;Parent=MsG0880046486.01.T01
Chr8 CDS 73945120 73945194 73945120 ID=MsG0880046486.01.T01:cds;Parent=MsG0880046486.01.T01
Chr8 CDS 73944890 73944994 73944890 ID=MsG0880046486.01.T01:cds;Parent=MsG0880046486.01.T01
Chr8 three_prime_UTR 73944558 73944889 73944558 ID=MsG0880046486.01.T01:three_prime_utr;Parent=MsG0880046486.01.T01
Gene Sequence

>MsG0880046486.01.T01

ATGTCTGACGAAGAGCACCAATTTGAATCTAAGGCCGATGCCGGTGCTTCCAAAACTTATCCTCAACAAGCTGGTACCATCCGTAAGAATGGATACATAGTTATTAAAAACCGTCCATGCAAGGTTGTTGAAGTTTCGACTTCGAAGACCGGGAAGCATGGACATGCCAAGTGTCATTTTGTTGCTATTGATATCTTCACTTCAAAGAAGCTTGAGGATATCGTGCCGTCTTCACATAATTGTGATGTTCCTCACGTGAATCGTACCGATTATCAGCTCATTGACATTTCTGAAGATGGATTTGTGAGCTTGCTCACTGAAAATGGAAACACTAAGGATGACCTCAAACTTCCCACCGATGACAGTCTGCTCACTCAGATCAAGGATGGATTTGCTGAGGGAAAGGACCTTGTTGTATCCGTGATGTCTGCTATGGGTGAGGAGCAGATCTGTGCTTTGAAGGATATTGGTGGAAAGAACTAA

Protein sequence

>MsG0880046486.01.T01

MSDEEHQFESKADAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAIDIFTSKKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGFVSLLTENGNTKDDLKLPTDDSLLTQIKDGFAEGKDLVVSVMSAMGEEQICALKDIGGKN*