AlfalfaGEDB | MsG0580024540.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024540.01

KEGG: K02951
GO: GO:0000723 GO:0003678 GO:0003735 GO:0005622 GO:0005840 GO:0006281 GO:0006412
PFAM: PF01248 PF05970

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024540.01.T01	XP_003611335.1	99.281	139	1	0	75	213	4	142	5.23E-94	281

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024540.01.T01	Q9XHS0	75	136	29	1	83	213	8	143	1.86E-68	209

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024540.01.T01	G7JXD6	99.281	139	1	0	75	213	4	142	2.50e-94	281

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048040.01	MsG0580024540.01	0.828341	9.487575e-55	9.403235e-52
MsG0080048922.01	MsG0580024540.01	0.865482	5.717899e-65	1.863222e-61
MsG0180000096.01	MsG0580024540.01	0.840646	7.671095e-58	1.099714e-54
MsG0180000118.01	MsG0580024540.01	0.882266	1.203233e-70	7.281254e-67
MsG0180001169.01	MsG0580024540.01	0.837267	5.749820e-57	7.428885e-54
MsG0180001829.01	MsG0580024540.01	0.851310	9.647410e-61	1.944803e-57
MsG0180002738.01	MsG0580024540.01	0.818069	2.375888e-52	1.760354e-49
MsG0180003585.01	MsG0580024540.01	0.865566	5.383190e-65	1.759527e-61
MsG0180003897.01	MsG0580024540.01	0.899565	1.748193e-77	2.173778e-73
MsG0180004829.01	MsG0580024540.01	0.867056	1.813385e-65	6.245544e-62

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PPI

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Gene1	Gene2	Type
MsG0180001832.01	MsG0580024540.01	PPI
MsG0380016379.01	MsG0580024540.01	PPI
MsG0480018164.01	MsG0580024540.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024540.01.T01	MTR_5g012890	99.281	139	1	0	75	213	4	142	6.33e-98	281
MsG0580024540.01.T01	MTR_8g093770	94.444	126	7	0	88	213	18	143	1.65e-85	249
MsG0580024540.01.T01	MTR_4g006830	94.444	126	7	0	88	213	19	144	2.63e-84	246
MsG0580024540.01.T01	MTR_5g012890	96.154	104	4	0	75	178	4	107	9.58e-68	203
MsG0580024540.01.T01	MTR_8g019090	84.314	51	8	0	1	51	59	109	1.32e-22	91.7
MsG0580024540.01.T01	MTR_4g073510	68.627	51	14	1	75	123	4	54	1.08e-13	69.3
MsG0580024540.01.T01	MTR_4g050743	50.000	54	27	0	1	54	94	147	4.92e-12	62.8
MsG0580024540.01.T01	MTR_7g050355	54.000	50	23	0	1	50	502	551	7.46e-12	64.3
MsG0580024540.01.T01	MTR_6g024100	57.143	49	21	0	2	50	150	198	1.76e-11	62.0
MsG0580024540.01.T01	MTR_1g057360	54.000	50	23	0	1	50	221	270	3.26e-11	62.0

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024540.01.T01	AT2G32060	74.400	125	31	1	89	213	21	144	2.24e-65	199
MsG0580024540.01.T01	AT2G32060	74.400	125	31	1	89	213	21	144	2.24e-65	199
MsG0580024540.01.T01	AT2G32060	74.400	125	31	1	89	213	21	144	2.24e-65	199
MsG0580024540.01.T01	AT1G15930	74.400	125	31	1	89	213	21	144	3.72e-63	193
MsG0580024540.01.T01	AT1G15930	74.400	125	31	1	89	213	21	144	3.72e-63	193

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Find 49 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCAGTCATGATGTCCAT+TGG	0.589394	5:-6171101	None:intergenic
AACATTGTGCTCTGCGCAGA+GGG	0.503587	5:-6171270	None:intergenic
AAGACCCTTGGAGAGTGGGC+TGG	0.538691	5:+6171317	MsG0580024540.01.T01:CDS
AGAAAGTCTCGTGCTTATGG+TGG	0.620799	5:+6171137	MsG0580024540.01.T01:CDS
ATCAGGTTGGTCACAATCTT+CGG	0.412966	5:-6171228	None:intergenic
ATGATGTCCATTGGCTCGCC+TGG	0.437864	5:-6171092	None:intergenic
ATGCTCTTGTGTGGTTGTCA+GGG	0.586706	5:+6171565	MsG0580024540.01.T01:CDS
ATGCTGCAAAACAACATTGT+AGG	0.363171	5:-6171700	None:intergenic
ATTTAGACCCGTGCGATGCA+CGG	0.505953	5:-6169916	None:intergenic
CAACCTTGAGATTTAACTCA+TGG	0.483340	5:-6168738	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAATAAGACAATGCAAACG+GGG	+	Chr5:6170695-6170714	MsG0580024540.01.T01:intron	30.0%
AAGACACAATATTGGACAAA+AGG	+	Chr5:6168874-6168893	MsG0580024540.01.T01:intron	30.0%
AAGTACGAAATTCCTCATTT+GGG	-	Chr5:6168838-6168857	None:intergenic	30.0%
ACAAGGAATTGGATAAAACT+AGG	-	Chr5:6171365-6171384	None:intergenic	30.0%
AGATAACTGAATCTGATATC+CGG	+	Chr5:6170794-6170813	MsG0580024540.01.T01:intron	30.0%
ATCAACCAAGAAATATGTCA+GGG	+	Chr5:6170263-6170282	MsG0580024540.01.T01:intron	30.0%
ATGACTATCAATAAGAGTCA+AGG	+	Chr5:6168995-6169014	MsG0580024540.01.T01:intron	30.0%
ATTGGAATTAGCAAAAGTGA+TGG	+	Chr5:6169630-6169649	MsG0580024540.01.T01:intron	30.0%
CAACTTATGAAAACAGCTTA+TGG	-	Chr5:6170448-6170467	None:intergenic	30.0%
CTGCATAAAACAAAATGGAA+CGG	-	Chr5:6170220-6170239	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	6168607	6171883	6168607	ID=MsG0580024540.01;Name=MsG0580024540.01
Chr5	mRNA	6168607	6171883	6168607	ID=MsG0580024540.01.T01;Parent=MsG0580024540.01;Name=MsG0580024540.01.T01;_AED=0.42;_eAED=0.42;_QI=0\|0.5\|0.6\|0.8\|0.75\|0.6\|5\|146\|213
Chr5	exon	6168607	6168756	6168607	ID=MsG0580024540.01.T01:exon:25744;Parent=MsG0580024540.01.T01
Chr5	exon	6169900	6169970	6169900	ID=MsG0580024540.01.T01:exon:25745;Parent=MsG0580024540.01.T01
Chr5	exon	6171046	6171340	6171046	ID=MsG0580024540.01.T01:exon:25746;Parent=MsG0580024540.01.T01
Chr5	exon	6171521	6171586	6171521	ID=MsG0580024540.01.T01:exon:25747;Parent=MsG0580024540.01.T01
Chr5	exon	6171678	6171883	6171678	ID=MsG0580024540.01.T01:exon:25748;Parent=MsG0580024540.01.T01
Chr5	CDS	6168607	6168756	6168607	ID=MsG0580024540.01.T01:cds;Parent=MsG0580024540.01.T01
Chr5	CDS	6169900	6169970	6169900	ID=MsG0580024540.01.T01:cds;Parent=MsG0580024540.01.T01
Chr5	CDS	6171046	6171340	6171046	ID=MsG0580024540.01.T01:cds;Parent=MsG0580024540.01.T01
Chr5	CDS	6171521	6171586	6171521	ID=MsG0580024540.01.T01:cds;Parent=MsG0580024540.01.T01
Chr5	CDS	6171678	6171737	6171678	ID=MsG0580024540.01.T01:cds;Parent=MsG0580024540.01.T01
Chr5	three_prime_UTR	6171738	6171883	6171738	ID=MsG0580024540.01.T01:three_prime_utr;Parent=MsG0580024540.01.T01

Gene Sequence

>MsG0580024540.01.T01

ATGATTCCTGGTGAGAAAAAAATATACTTATGTGCTGATAGTCCGTCTACACATGATGAAAATATTAATAGTCCTGATGAGATTCTCACTCCAGAATTTCTGAACACCGTAAAATCTTCAGGGTCGCCGAACCATGAGTTAAATCTCAAGTGCCATAATTTACTTCCCGTGCATCGCACGGGTCTAAATACTAGCATTATCAAAACCATTTACTTTGAGACTGAAGAAGAGGTTGTTGCAGTTGAGCCTGTTGTTGCTGCTGCAATTCCAGGCGAGCCAATGGACATCATGACTGCTTTACAGCTTGTCCTTAGAAAGTCTCGTGCTTATGGTGGTCTTGCACGAGGTCTTCATGAAGGTGCAAAAGTGATTGAGAAGCATGCTGCACAGCTTTGTGTTCTAGCCGAAGATTGTGACCAACCTGATTATGTCAAATTGGTGAAAGCCCTCTGCGCAGAGCACAATGTTAGCTTGTTGACAGTTCCAAGTGCAAAGACCCTTGGAGAGTGGGCTGGCTTGTGCAAGATTGATTCAGAAGGTAAAGCTAGGAAGGTTACCGGATGCTCTTGTGTGGTTGTCAGGGATTTTGGGGAGGAACACGAAGCCTACAATGTTGTTTTGCAGCATGTAAAAGCTAACTAA

Protein sequence

>MsG0580024540.01.T01

MIPGEKKIYLCADSPSTHDENINSPDEILTPEFLNTVKSSGSPNHELNLKCHNLLPVHRTGLNTSIIKTIYFETEEEVVAVEPVVAAAIPGEPMDIMTALQLVLRKSRAYGGLARGLHEGAKVIEKHAAQLCVLAEDCDQPDYVKLVKALCAEHNVSLLTVPSAKTLGEWAGLCKIDSEGKARKVTGCSCVVVRDFGEEHEAYNVVLQHVKAN*