Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019195.01.T01 | XP_013447002.1 | 87.952 | 166 | 20 | 0 | 1 | 166 | 8 | 173 | 5.70E-103 | 303 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019195.01.T01 | P31110 | 61.635 | 159 | 55 | 3 | 9 | 166 | 24 | 177 | 2.69E-64 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019195.01.T01 | A0A072TU31 | 87.952 | 166 | 20 | 0 | 1 | 166 | 8 | 173 | 2.72e-103 | 303 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0480019191.01 | MsG0480019195.01 | 0.987739 | 2.326654e-171 | 1.255336e-164 |
| MsG0480019192.01 | MsG0480019195.01 | 0.954201 | 5.038913e-112 | 1.395922e-106 |
| MsG0480019193.01 | MsG0480019195.01 | 0.985222 | 6.681424e-163 | 2.625558e-156 |
| MsG0480019194.01 | MsG0480019195.01 | 0.981719 | 2.808649e-153 | 7.517312e-147 |
| MsG0480019195.01 | MsG0480021788.01 | 0.800263 | 1.564491e-48 | 7.252523e-46 |
| MsG0480019195.01 | MsG0480021789.01 | 0.807776 | 4.284835e-50 | 2.407648e-47 |
| MsG0480019195.01 | MsG0680031451.01 | 0.817044 | 4.043761e-52 | 2.913390e-49 |
| MsG0480019195.01 | MsG0780036613.01 | 0.823066 | 1.691929e-53 | 1.441649e-50 |
| MsG0480019195.01 | MsG0780039099.01 | 0.801182 | 1.015644e-48 | 4.819223e-46 |
| MsG0480019195.01 | MsG0880044626.01 | 0.811817 | 5.790530e-51 | 3.620453e-48 |
| MsG0280006552.01 | MsG0480019195.01 | 0.807164 | 5.779011e-50 | 3.195916e-47 |
| MsG0280006553.01 | MsG0480019195.01 | 0.804017 | 2.643311e-49 | 1.348268e-46 |
| MsG0280009901.01 | MsG0480019195.01 | 0.802651 | 5.070155e-49 | 2.497066e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019195.01.T01 | MTR_8g096920 | 87.952 | 166 | 20 | 0 | 1 | 166 | 8 | 173 | 6.91e-107 | 303 |
| MsG0480019195.01.T01 | MTR_8g096910 | 80.120 | 166 | 33 | 0 | 1 | 166 | 11 | 176 | 1.47e-100 | 287 |
| MsG0480019195.01.T01 | MTR_8g096900 | 47.685 | 216 | 62 | 5 | 1 | 166 | 34 | 248 | 1.30e-58 | 183 |
| MsG0480019195.01.T01 | MTR_5g010635 | 44.660 | 206 | 65 | 3 | 9 | 166 | 21 | 225 | 1.07e-48 | 157 |
| MsG0480019195.01.T01 | MTR_5g010640 | 51.748 | 143 | 66 | 2 | 6 | 146 | 20 | 161 | 1.29e-44 | 147 |
| MsG0480019195.01.T01 | MTR_2g067990 | 41.206 | 199 | 72 | 7 | 11 | 166 | 26 | 222 | 1.40e-33 | 125 |
| MsG0480019195.01.T01 | MTR_5g059200 | 48.305 | 118 | 52 | 4 | 10 | 122 | 26 | 139 | 6.69e-28 | 105 |
| MsG0480019195.01.T01 | MTR_6g079580 | 41.176 | 136 | 63 | 5 | 1 | 122 | 5 | 137 | 4.57e-27 | 102 |
| MsG0480019195.01.T01 | MTR_8g107140 | 44.348 | 115 | 53 | 4 | 16 | 122 | 31 | 142 | 1.48e-26 | 101 |
| MsG0480019195.01.T01 | MTR_1g021945 | 47.200 | 125 | 56 | 3 | 9 | 124 | 23 | 146 | 3.15e-26 | 100 |
| MsG0480019195.01.T01 | MTR_7g062610 | 41.259 | 143 | 70 | 5 | 6 | 139 | 17 | 154 | 3.51e-26 | 100 |
| MsG0480019195.01.T01 | MTR_4g073720 | 42.767 | 159 | 67 | 9 | 1 | 140 | 11 | 164 | 9.19e-26 | 100 |
| MsG0480019195.01.T01 | MTR_5g022350 | 34.419 | 215 | 79 | 10 | 12 | 166 | 31 | 243 | 1.81e-25 | 99.8 |
| MsG0480019195.01.T01 | MTR_2g067980 | 35.443 | 237 | 79 | 11 | 2 | 166 | 13 | 247 | 4.41e-25 | 100 |
| MsG0480019195.01.T01 | MTR_5g022350 | 45.669 | 127 | 60 | 7 | 7 | 127 | 91 | 214 | 5.09e-25 | 96.7 |
| MsG0480019195.01.T01 | MTR_8g075510 | 42.143 | 140 | 66 | 7 | 7 | 133 | 26 | 163 | 1.18e-24 | 96.3 |
| MsG0480019195.01.T01 | MTR_8g075510 | 41.497 | 147 | 68 | 8 | 7 | 140 | 26 | 167 | 2.04e-24 | 96.7 |
| MsG0480019195.01.T01 | MTR_3g111620 | 40.816 | 147 | 68 | 5 | 9 | 140 | 24 | 166 | 3.36e-24 | 95.1 |
| MsG0480019195.01.T01 | MTR_4g073730 | 42.958 | 142 | 67 | 7 | 1 | 129 | 13 | 153 | 5.07e-24 | 95.1 |
| MsG0480019195.01.T01 | MTR_8g037890 | 42.400 | 125 | 62 | 4 | 6 | 121 | 20 | 143 | 6.72e-24 | 95.5 |
| MsG0480019195.01.T01 | MTR_8g075550 | 39.181 | 171 | 80 | 7 | 11 | 165 | 27 | 189 | 8.25e-24 | 95.1 |
| MsG0480019195.01.T01 | MTR_4g073730 | 42.958 | 142 | 67 | 7 | 1 | 129 | 13 | 153 | 8.27e-24 | 95.5 |
| MsG0480019195.01.T01 | MTR_1g025420 | 42.336 | 137 | 61 | 5 | 14 | 133 | 25 | 160 | 2.60e-23 | 92.8 |
| MsG0480019195.01.T01 | MTR_8g036215 | 37.654 | 162 | 81 | 7 | 1 | 147 | 9 | 165 | 5.09e-23 | 91.7 |
| MsG0480019195.01.T01 | MTR_1g025420 | 42.336 | 137 | 61 | 5 | 14 | 133 | 25 | 160 | 6.89e-23 | 92.8 |
| MsG0480019195.01.T01 | MTR_5g022310 | 38.356 | 146 | 71 | 5 | 6 | 133 | 20 | 164 | 7.03e-23 | 91.7 |
| MsG0480019195.01.T01 | MTR_5g022310 | 37.908 | 153 | 73 | 6 | 6 | 140 | 20 | 168 | 1.13e-22 | 92.0 |
| MsG0480019195.01.T01 | MTR_3g114030 | 42.735 | 117 | 57 | 4 | 14 | 121 | 9 | 124 | 4.21e-22 | 89.4 |
| MsG0480019195.01.T01 | MTR_3g068015 | 40.876 | 137 | 66 | 7 | 13 | 139 | 29 | 160 | 4.62e-22 | 89.4 |
| MsG0480019195.01.T01 | MTR_8g056820 | 41.600 | 125 | 63 | 4 | 6 | 121 | 20 | 143 | 8.99e-22 | 88.6 |
| MsG0480019195.01.T01 | MTR_2g068030 | 42.623 | 122 | 58 | 5 | 11 | 121 | 22 | 142 | 2.00e-21 | 87.8 |
| MsG0480019195.01.T01 | MTR_2g069660 | 31.897 | 232 | 89 | 8 | 3 | 166 | 13 | 243 | 3.94e-21 | 86.7 |
| MsG0480019195.01.T01 | MTR_2g068655 | 37.126 | 167 | 83 | 7 | 9 | 157 | 28 | 190 | 1.79e-20 | 86.3 |
| MsG0480019195.01.T01 | MTR_6g009480 | 37.681 | 138 | 75 | 5 | 6 | 133 | 23 | 159 | 2.94e-20 | 85.1 |
| MsG0480019195.01.T01 | MTR_4g063630 | 36.641 | 131 | 73 | 4 | 1 | 122 | 18 | 147 | 5.85e-20 | 84.0 |
| MsG0480019195.01.T01 | MTR_7g102380 | 38.235 | 136 | 67 | 7 | 1 | 121 | 4 | 137 | 5.89e-20 | 83.6 |
| MsG0480019195.01.T01 | MTR_1g062630 | 37.692 | 130 | 70 | 6 | 2 | 122 | 12 | 139 | 1.13e-18 | 80.5 |
| MsG0480019195.01.T01 | MTR_1g062350 | 33.908 | 174 | 82 | 7 | 2 | 166 | 12 | 161 | 1.48e-18 | 80.1 |
| MsG0480019195.01.T01 | MTR_5g023850 | 32.308 | 195 | 94 | 8 | 9 | 166 | 23 | 216 | 1.62e-18 | 79.3 |
| MsG0480019195.01.T01 | MTR_1g062370 | 33.333 | 174 | 83 | 7 | 2 | 166 | 12 | 161 | 5.10e-18 | 78.6 |
| MsG0480019195.01.T01 | MTR_1g062340 | 37.209 | 129 | 70 | 5 | 2 | 121 | 12 | 138 | 1.07e-17 | 77.8 |
| MsG0480019195.01.T01 | MTR_1g062590 | 35.135 | 148 | 81 | 6 | 2 | 140 | 12 | 153 | 1.57e-17 | 77.0 |
| MsG0480019195.01.T01 | MTR_1g062660 | 32.955 | 176 | 83 | 8 | 2 | 166 | 10 | 161 | 7.18e-17 | 75.5 |
| MsG0480019195.01.T01 | MTR_1g062690 | 30.058 | 173 | 66 | 7 | 2 | 166 | 12 | 137 | 1.60e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019195.01.T01 | AT4G11650 | 45.238 | 210 | 67 | 3 | 4 | 166 | 15 | 223 | 1.25e-53 | 170 |
| MsG0480019195.01.T01 | AT1G77700 | 51.261 | 119 | 50 | 3 | 8 | 122 | 57 | 171 | 2.31e-33 | 120 |
| MsG0480019195.01.T01 | AT1G77700 | 51.261 | 119 | 50 | 3 | 8 | 122 | 85 | 199 | 2.73e-33 | 120 |
| MsG0480019195.01.T01 | AT1G77700 | 51.261 | 119 | 50 | 3 | 8 | 122 | 57 | 171 | 2.84e-33 | 120 |
| MsG0480019195.01.T01 | AT1G75050 | 47.541 | 122 | 57 | 2 | 6 | 121 | 19 | 139 | 1.72e-28 | 106 |
| MsG0480019195.01.T01 | AT1G75030 | 44.531 | 128 | 63 | 3 | 1 | 121 | 13 | 139 | 4.99e-27 | 102 |
| MsG0480019195.01.T01 | AT4G36010 | 50.847 | 118 | 47 | 5 | 14 | 121 | 25 | 141 | 2.45e-26 | 101 |
| MsG0480019195.01.T01 | AT4G36010 | 50.847 | 118 | 47 | 5 | 14 | 121 | 25 | 141 | 2.45e-26 | 101 |
| MsG0480019195.01.T01 | AT1G18250 | 45.455 | 121 | 55 | 4 | 9 | 121 | 18 | 135 | 4.86e-26 | 99.8 |
| MsG0480019195.01.T01 | AT1G73620 | 44.628 | 121 | 56 | 4 | 9 | 121 | 39 | 156 | 6.04e-26 | 99.8 |
| MsG0480019195.01.T01 | AT4G38660 | 41.060 | 151 | 68 | 7 | 2 | 133 | 17 | 165 | 7.93e-26 | 101 |
| MsG0480019195.01.T01 | AT1G18250 | 44.262 | 122 | 57 | 4 | 8 | 121 | 18 | 136 | 1.11e-25 | 98.6 |
| MsG0480019195.01.T01 | AT4G38660 | 41.667 | 144 | 66 | 5 | 6 | 133 | 2 | 143 | 2.39e-25 | 99.4 |
| MsG0480019195.01.T01 | AT1G75040 | 33.478 | 230 | 86 | 7 | 1 | 166 | 13 | 239 | 4.98e-25 | 97.1 |
| MsG0480019195.01.T01 | AT1G75800 | 40.268 | 149 | 75 | 6 | 1 | 139 | 11 | 155 | 8.36e-25 | 98.2 |
| MsG0480019195.01.T01 | AT2G17860 | 47.458 | 118 | 51 | 5 | 14 | 121 | 25 | 141 | 3.55e-23 | 92.4 |
| MsG0480019195.01.T01 | AT1G19320 | 39.850 | 133 | 68 | 5 | 1 | 122 | 15 | 146 | 8.05e-23 | 91.3 |
| MsG0480019195.01.T01 | AT4G38670 | 44.275 | 131 | 60 | 6 | 2 | 121 | 13 | 141 | 8.94e-23 | 92.0 |
| MsG0480019195.01.T01 | AT4G38670 | 44.275 | 131 | 60 | 6 | 2 | 121 | 13 | 141 | 8.94e-23 | 92.0 |
| MsG0480019195.01.T01 | AT5G38280 | 37.778 | 135 | 72 | 5 | 14 | 139 | 27 | 158 | 1.16e-22 | 94.0 |
| MsG0480019195.01.T01 | AT4G38670 | 44.094 | 127 | 58 | 6 | 6 | 121 | 1 | 125 | 1.91e-22 | 90.9 |
| MsG0480019195.01.T01 | AT5G24620 | 40.299 | 134 | 70 | 4 | 3 | 127 | 16 | 148 | 2.89e-22 | 92.4 |
| MsG0480019195.01.T01 | AT5G24620 | 40.741 | 135 | 68 | 5 | 3 | 127 | 92 | 224 | 3.39e-22 | 92.4 |
| MsG0480019195.01.T01 | AT5G24620 | 41.732 | 127 | 62 | 5 | 3 | 119 | 71 | 195 | 3.54e-22 | 92.4 |
| MsG0480019195.01.T01 | AT5G02140 | 36.842 | 171 | 90 | 8 | 1 | 154 | 7 | 176 | 4.93e-22 | 89.4 |
| MsG0480019195.01.T01 | AT5G02140 | 36.842 | 171 | 90 | 8 | 1 | 154 | 7 | 176 | 5.36e-22 | 90.1 |
| MsG0480019195.01.T01 | AT5G38280 | 40.152 | 132 | 65 | 6 | 1 | 119 | 10 | 140 | 6.86e-22 | 91.7 |
| MsG0480019195.01.T01 | AT1G20030 | 42.735 | 117 | 57 | 4 | 14 | 121 | 5 | 120 | 1.45e-21 | 89.0 |
| MsG0480019195.01.T01 | AT1G20030 | 42.735 | 117 | 57 | 4 | 14 | 121 | 22 | 137 | 1.77e-21 | 89.0 |
| MsG0480019195.01.T01 | AT5G40020 | 33.544 | 158 | 85 | 6 | 7 | 148 | 23 | 176 | 2.61e-21 | 87.4 |
| MsG0480019195.01.T01 | AT4G24180 | 38.235 | 136 | 68 | 6 | 4 | 124 | 25 | 159 | 5.65e-20 | 84.0 |
| MsG0480019195.01.T01 | AT4G24180 | 38.235 | 136 | 68 | 6 | 4 | 124 | 25 | 159 | 9.52e-20 | 84.3 |
| MsG0480019195.01.T01 | AT4G24180 | 38.235 | 136 | 68 | 6 | 4 | 124 | 25 | 159 | 1.39e-19 | 84.0 |
| MsG0480019195.01.T01 | AT2G28790 | 37.121 | 132 | 71 | 6 | 16 | 136 | 56 | 186 | 2.07e-19 | 82.8 |
| MsG0480019195.01.T01 | AT2G28790 | 37.121 | 132 | 71 | 6 | 16 | 136 | 31 | 161 | 2.49e-19 | 82.0 |
| MsG0480019195.01.T01 | AT4G24180 | 39.062 | 128 | 62 | 6 | 12 | 124 | 28 | 154 | 9.33e-19 | 80.9 |
| MsG0480019195.01.T01 | AT4G24180 | 39.062 | 128 | 62 | 6 | 12 | 124 | 28 | 154 | 1.12e-18 | 81.6 |
| MsG0480019195.01.T01 | AT4G24180 | 37.500 | 136 | 69 | 6 | 4 | 124 | 20 | 154 | 1.71e-18 | 80.9 |
| MsG0480019195.01.T01 | AT1G70250 | 34.074 | 135 | 79 | 5 | 14 | 140 | 147 | 279 | 5.37e-16 | 75.1 |
| MsG0480019195.01.T01 | AT4G18250 | 27.570 | 214 | 90 | 10 | 16 | 166 | 61 | 272 | 4.36e-11 | 60.8 |
Find 58 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAACTTCATCAGGTCGAATT+TGG | 0.148264 | 4:-15816319 | MsG0480019195.01.T01:CDS |
| ACTTAACGGTGGAAACAATT+TGG | 0.219094 | 4:-15816177 | MsG0480019195.01.T01:CDS |
| AACTTCATCAGGTCGAATTT+GGG | 0.288054 | 4:-15816318 | MsG0480019195.01.T01:CDS |
| TACTTTGTTGCATCCATTAG+AGG | 0.301618 | 4:+15816097 | None:intergenic |
| CAACTTAGTTCAACCTCTAA+TGG | 0.319722 | 4:-15816110 | MsG0480019195.01.T01:CDS |
| AAGAGTGAGCGGACTCTTAC+CGG | 0.332233 | 4:+15816211 | None:intergenic |
| ATTGATTTATCTGTGATTGA+TGG | 0.357832 | 4:-15816149 | MsG0480019195.01.T01:CDS |
| CCACCACTGGGTGTGGCCGC+TGG | 0.368182 | 4:+15816392 | None:intergenic |
| CAACTGCCGACCTCCACCAC+TGG | 0.374879 | 4:+15816379 | None:intergenic |
| TCCACCGTTAAGTGTGAATT+CGG | 0.380057 | 4:+15816187 | None:intergenic |
| GGTTGCACCTTCGACGGTTC+AGG | 0.394877 | 4:-15816287 | MsG0480019195.01.T01:CDS |
| GGCAATGGTAATTGTCAAAC+TGG | 0.406321 | 4:-15816266 | MsG0480019195.01.T01:CDS |
| CTTACCGAATTCACACTTAA+CGG | 0.429312 | 4:-15816191 | MsG0480019195.01.T01:CDS |
| GTCCTCAGTTGCAGCTTATC+CGG | 0.434820 | 4:-15816230 | MsG0480019195.01.T01:CDS |
| TTGGGTTGATGATAAGCATC+AGG | 0.436290 | 4:+15816050 | None:intergenic |
| GGTCGAATTTGGGGCCGAAC+TGG | 0.442440 | 4:-15816308 | MsG0480019195.01.T01:CDS |
| ACAAAAGACAACTTTGTAGT+TGG | 0.448816 | 4:+15815995 | None:intergenic |
| ATCCCAGCTGGAACTTCATC+AGG | 0.462218 | 4:-15816329 | MsG0480019195.01.T01:CDS |
| GAGGTTGAACTAAGTTGCAT+TGG | 0.462843 | 4:+15816116 | None:intergenic |
| GCCACACGGTGTAGCCGCAT+TGG | 0.474171 | 4:+15816414 | None:intergenic |
| CCGACCTCCACCACTGGGTG+TGG | 0.490136 | 4:+15816385 | None:intergenic |
| TGATATCTGCTCAGGCAGCT+AGG | 0.492068 | 4:-15816451 | MsG0480019195.01.T01:CDS |
| AACTGCCGACCTCCACCACT+GGG | 0.492264 | 4:+15816380 | None:intergenic |
| CCAGAGTCCATGTTTGACCT+TGG | 0.495410 | 4:+15816354 | None:intergenic |
| CGTCGAAGGTGCAACCAGTT+CGG | 0.498999 | 4:+15816294 | None:intergenic |
| CGACCTGATGAAGTTCCAGC+TGG | 0.502812 | 4:+15816326 | None:intergenic |
| ACTTTGTAGTTGGTACCACC+TGG | 0.508562 | 4:+15816005 | None:intergenic |
| CGAACTGGTTGCACCTTCGA+CGG | 0.509502 | 4:-15816293 | MsG0480019195.01.T01:CDS |
| TCTCTGCTTGATATCTGCTC+AGG | 0.510960 | 4:-15816459 | MsG0480019195.01.T01:CDS |
| GCGGCCACACCCAGTGGTGG+AGG | 0.514387 | 4:-15816389 | MsG0480019195.01.T01:CDS |
| GCAACAAAGTATTAAACTGC+GGG | 0.515893 | 4:-15816085 | MsG0480019195.01.T01:CDS |
| ACCTTCGACGGTTCAGGCAA+TGG | 0.516066 | 4:-15816281 | MsG0480019195.01.T01:CDS |
| GGGTGTGGCCGCTGGCCACA+CGG | 0.516945 | 4:+15816400 | None:intergenic |
| TGGACTCTGGACATCCCAGC+TGG | 0.517689 | 4:-15816341 | MsG0480019195.01.T01:CDS |
| CAGTGGTGGAGGTCGGCAGT+TGG | 0.546103 | 4:-15816378 | MsG0480019195.01.T01:CDS |
| CCAGCGGCCACACCCAGTGG+TGG | 0.568387 | 4:-15816392 | MsG0480019195.01.T01:CDS |
| CCAAGGTCAAACATGGACTC+TGG | 0.568915 | 4:-15816354 | MsG0480019195.01.T01:CDS |
| CGGCTACACCGTGTGGCCAG+CGG | 0.572344 | 4:-15816408 | MsG0480019195.01.T01:CDS |
| CCACACCCAGTGGTGGAGGT+CGG | 0.594695 | 4:-15816385 | MsG0480019195.01.T01:CDS |
| TACCGGATAAGCTGCAACTG+AGG | 0.596730 | 4:+15816228 | None:intergenic |
| ACCATTGCCTGAACCGTCGA+AGG | 0.597990 | 4:+15816280 | None:intergenic |
| TGGCCAGCGGCCACACCCAG+TGG | 0.600466 | 4:-15816395 | MsG0480019195.01.T01:CDS |
| GTTAAAACGAATACATGCCC+AGG | 0.604035 | 4:-15816023 | MsG0480019195.01.T01:CDS |
| AGTTGGACCAAGGTCAAACA+TGG | 0.621156 | 4:-15816361 | MsG0480019195.01.T01:CDS |
| ACTTCATCAGGTCGAATTTG+GGG | 0.621329 | 4:-15816317 | MsG0480019195.01.T01:CDS |
| ACCAATGCGGCTACACCGTG+TGG | 0.625115 | 4:-15816415 | MsG0480019195.01.T01:CDS |
| GGAGGTCGGCAGTTGGACCA+AGG | 0.633114 | 4:-15816371 | MsG0480019195.01.T01:CDS |
| GACCTGATGAAGTTCCAGCT+GGG | 0.635258 | 4:+15816327 | None:intergenic |
| GTGAATTCGGTAAGAGTGAG+CGG | 0.641384 | 4:+15816200 | None:intergenic |
| TGGGTTGATGATAAGCATCA+GGG | 0.651917 | 4:+15816051 | None:intergenic |
| TGCAACAAAGTATTAAACTG+CGG | 0.658121 | 4:-15816086 | MsG0480019195.01.T01:CDS |
| CTTTGTAGTTGGTACCACCT+GGG | 0.662098 | 4:+15816006 | None:intergenic |
| AATTGTCAAACTGGTGATTG+CGG | 0.670033 | 4:-15816257 | MsG0480019195.01.T01:CDS |
| ACCGAATTCACACTTAACGG+TGG | 0.677282 | 4:-15816188 | MsG0480019195.01.T01:CDS |
| TGTCAAACTGGTGATTGCGG+TGG | 0.712111 | 4:-15816254 | MsG0480019195.01.T01:CDS |
| CAACAAAGTATTAAACTGCG+GGG | 0.718105 | 4:-15816084 | MsG0480019195.01.T01:CDS |
| AAAACGAATACATGCCCAGG+TGG | 0.726701 | 4:-15816020 | MsG0480019195.01.T01:CDS |
| TTTGACATTGTAAACCAATG+CGG | 0.728342 | 4:-15816428 | MsG0480019195.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGATTTATCTGTGATTGA+TGG | - | Chr4:15816307-15816326 | MsG0480019195.01.T01:CDS | 25.0% |
| !!! | GTATTCGTTTTAACATCATT+GGG | + | Chr4:15816427-15816446 | None:intergenic | 25.0% |
| !!! | TGTATTCGTTTTAACATCAT+TGG | + | Chr4:15816428-15816447 | None:intergenic | 25.0% |
| ACAAAAGACAACTTTGTAGT+TGG | + | Chr4:15816464-15816483 | None:intergenic | 30.0% | |
| TTTGACATTGTAAACCAATG+CGG | - | Chr4:15816028-15816047 | MsG0480019195.01.T01:CDS | 30.0% | |
| ! | TGCAACAAAGTATTAAACTG+CGG | - | Chr4:15816370-15816389 | MsG0480019195.01.T01:CDS | 30.0% |
| AACTTCATCAGGTCGAATTT+GGG | - | Chr4:15816138-15816157 | MsG0480019195.01.T01:CDS | 35.0% | |
| AATTGTCAAACTGGTGATTG+CGG | - | Chr4:15816199-15816218 | MsG0480019195.01.T01:CDS | 35.0% | |
| ACTTAACGGTGGAAACAATT+TGG | - | Chr4:15816279-15816298 | MsG0480019195.01.T01:CDS | 35.0% | |
| CAACTTAGTTCAACCTCTAA+TGG | - | Chr4:15816346-15816365 | MsG0480019195.01.T01:CDS | 35.0% | |
| CTTACCGAATTCACACTTAA+CGG | - | Chr4:15816265-15816284 | MsG0480019195.01.T01:CDS | 35.0% | |
| TACTTTGTTGCATCCATTAG+AGG | + | Chr4:15816362-15816381 | None:intergenic | 35.0% | |
| ! | CAACAAAGTATTAAACTGCG+GGG | - | Chr4:15816372-15816391 | MsG0480019195.01.T01:CDS | 35.0% |
| ! | GCAACAAAGTATTAAACTGC+GGG | - | Chr4:15816371-15816390 | MsG0480019195.01.T01:CDS | 35.0% |
| ACTTCATCAGGTCGAATTTG+GGG | - | Chr4:15816139-15816158 | MsG0480019195.01.T01:CDS | 40.0% | |
| GAACTTCATCAGGTCGAATT+TGG | - | Chr4:15816137-15816156 | MsG0480019195.01.T01:CDS | 40.0% | |
| GAGGTTGAACTAAGTTGCAT+TGG | + | Chr4:15816343-15816362 | None:intergenic | 40.0% | |
| GGCAATGGTAATTGTCAAAC+TGG | - | Chr4:15816190-15816209 | MsG0480019195.01.T01:CDS | 40.0% | |
| GTTAAAACGAATACATGCCC+AGG | - | Chr4:15816433-15816452 | MsG0480019195.01.T01:CDS | 40.0% | |
| TCCACCGTTAAGTGTGAATT+CGG | + | Chr4:15816272-15816291 | None:intergenic | 40.0% | |
| !! | TGGGTTGATGATAAGCATCA+GGG | + | Chr4:15816408-15816427 | None:intergenic | 40.0% |
| !! | TTGGGTTGATGATAAGCATC+AGG | + | Chr4:15816409-15816428 | None:intergenic | 40.0% |
| AAAACGAATACATGCCCAGG+TGG | - | Chr4:15816436-15816455 | MsG0480019195.01.T01:CDS | 45.0% | |
| ACCGAATTCACACTTAACGG+TGG | - | Chr4:15816268-15816287 | MsG0480019195.01.T01:CDS | 45.0% | |
| AGTTGGACCAAGGTCAAACA+TGG | - | Chr4:15816095-15816114 | MsG0480019195.01.T01:CDS | 45.0% | |
| GTGAATTCGGTAAGAGTGAG+CGG | + | Chr4:15816259-15816278 | None:intergenic | 45.0% | |
| TCTCTGCTTGATATCTGCTC+AGG | - | Chr4:15815997-15816016 | MsG0480019195.01.T01:CDS | 45.0% | |
| ! | ACTTTGTAGTTGGTACCACC+TGG | + | Chr4:15816454-15816473 | None:intergenic | 45.0% |
| ! | CTTTGTAGTTGGTACCACCT+GGG | + | Chr4:15816453-15816472 | None:intergenic | 45.0% |
| ATCCCAGCTGGAACTTCATC+AGG | - | Chr4:15816127-15816146 | MsG0480019195.01.T01:CDS | 50.0% | |
| CCAAGGTCAAACATGGACTC+TGG | - | Chr4:15816102-15816121 | MsG0480019195.01.T01:CDS | 50.0% | |
| CCAGAGTCCATGTTTGACCT+TGG | + | Chr4:15816105-15816124 | None:intergenic | 50.0% | |
| GACCTGATGAAGTTCCAGCT+GGG | + | Chr4:15816132-15816151 | None:intergenic | 50.0% | |
| GTCCTCAGTTGCAGCTTATC+CGG | - | Chr4:15816226-15816245 | MsG0480019195.01.T01:CDS | 50.0% | |
| TACCGGATAAGCTGCAACTG+AGG | + | Chr4:15816231-15816250 | None:intergenic | 50.0% | |
| TGATATCTGCTCAGGCAGCT+AGG | - | Chr4:15816005-15816024 | MsG0480019195.01.T01:CDS | 50.0% | |
| TGTCAAACTGGTGATTGCGG+TGG | - | Chr4:15816202-15816221 | MsG0480019195.01.T01:CDS | 50.0% | |
| ! | AAGAGTGAGCGGACTCTTAC+CGG | + | Chr4:15816248-15816267 | None:intergenic | 50.0% |
| ACCATTGCCTGAACCGTCGA+AGG | + | Chr4:15816179-15816198 | None:intergenic | 55.0% | |
| ACCTTCGACGGTTCAGGCAA+TGG | - | Chr4:15816175-15816194 | MsG0480019195.01.T01:CDS | 55.0% | |
| CGAACTGGTTGCACCTTCGA+CGG | - | Chr4:15816163-15816182 | MsG0480019195.01.T01:CDS | 55.0% | |
| CGACCTGATGAAGTTCCAGC+TGG | + | Chr4:15816133-15816152 | None:intergenic | 55.0% | |
| ! | CGTCGAAGGTGCAACCAGTT+CGG | + | Chr4:15816165-15816184 | None:intergenic | 55.0% |
| AACTGCCGACCTCCACCACT+GGG | + | Chr4:15816079-15816098 | None:intergenic | 60.0% | |
| ACCAATGCGGCTACACCGTG+TGG | - | Chr4:15816041-15816060 | MsG0480019195.01.T01:CDS | 60.0% | |
| GGTTGCACCTTCGACGGTTC+AGG | - | Chr4:15816169-15816188 | MsG0480019195.01.T01:CDS | 60.0% | |
| TGGACTCTGGACATCCCAGC+TGG | - | Chr4:15816115-15816134 | MsG0480019195.01.T01:CDS | 60.0% | |
| ! | GGTCGAATTTGGGGCCGAAC+TGG | - | Chr4:15816148-15816167 | MsG0480019195.01.T01:CDS | 60.0% |
| CAACTGCCGACCTCCACCAC+TGG | + | Chr4:15816080-15816099 | None:intergenic | 65.0% | |
| GCCACACGGTGTAGCCGCAT+TGG | + | Chr4:15816045-15816064 | None:intergenic | 65.0% | |
| GGAGGTCGGCAGTTGGACCA+AGG | - | Chr4:15816085-15816104 | MsG0480019195.01.T01:CDS | 65.0% | |
| ! | CAGTGGTGGAGGTCGGCAGT+TGG | - | Chr4:15816078-15816097 | MsG0480019195.01.T01:CDS | 65.0% |
| ! | CCACACCCAGTGGTGGAGGT+CGG | - | Chr4:15816071-15816090 | MsG0480019195.01.T01:CDS | 65.0% |
| CCGACCTCCACCACTGGGTG+TGG | + | Chr4:15816074-15816093 | None:intergenic | 70.0% | |
| CGGCTACACCGTGTGGCCAG+CGG | - | Chr4:15816048-15816067 | MsG0480019195.01.T01:CDS | 70.0% | |
| CCACCACTGGGTGTGGCCGC+TGG | + | Chr4:15816067-15816086 | None:intergenic | 75.0% | |
| CCAGCGGCCACACCCAGTGG+TGG | - | Chr4:15816064-15816083 | MsG0480019195.01.T01:CDS | 75.0% | |
| GCGGCCACACCCAGTGGTGG+AGG | - | Chr4:15816067-15816086 | MsG0480019195.01.T01:CDS | 75.0% | |
| GGGTGTGGCCGCTGGCCACA+CGG | + | Chr4:15816059-15816078 | None:intergenic | 75.0% | |
| TGGCCAGCGGCCACACCCAG+TGG | - | Chr4:15816061-15816080 | MsG0480019195.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 15815989 | 15816489 | 15815989 | ID=MsG0480019195.01;Name=MsG0480019195.01 |
| Chr4 | mRNA | 15815989 | 15816489 | 15815989 | ID=MsG0480019195.01.T01;Parent=MsG0480019195.01;Name=MsG0480019195.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|166 |
| Chr4 | exon | 15815989 | 15816489 | 15815989 | ID=MsG0480019195.01.T01:exon:5152;Parent=MsG0480019195.01.T01 |
| Chr4 | CDS | 15815989 | 15816489 | 15815989 | ID=MsG0480019195.01.T01:cds;Parent=MsG0480019195.01.T01 |
| Gene Sequence |
| Protein sequence |
