Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019603.01.T01 | XP_024636225.1 | 86.473 | 207 | 28 | 0 | 1 | 207 | 1 | 207 | 8.20E-126 | 365 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019603.01.T01 | A0A396I2E1 | 86.473 | 207 | 28 | 0 | 1 | 207 | 1 | 207 | 3.91e-126 | 365 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047797.01 | MsG0480019603.01 | 0.808356 | 3.223696e-50 | 1.839416e-47 |
| MsG0080048874.01 | MsG0480019603.01 | 0.820825 | 5.589557e-53 | 4.470611e-50 |
| MsG0480018513.01 | MsG0480019603.01 | 0.803843 | 2.872929e-49 | 1.458874e-46 |
| MsG0480019603.01 | MsG0780036080.01 | 0.802897 | 4.510385e-49 | 2.235458e-46 |
| MsG0480019603.01 | MsG0780036201.01 | 0.843345 | 1.483826e-58 | 2.315039e-55 |
| MsG0480019603.01 | MsG0780038691.01 | 0.822319 | 2.524597e-53 | 2.105963e-50 |
| MsG0480019603.01 | MsG0780039178.01 | 0.815369 | 9.579191e-52 | 6.591873e-49 |
| MsG0380015196.01 | MsG0480019603.01 | 0.824496 | 7.822573e-54 | 6.940321e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019603.01.T01 | MTR_2g066790 | 77.778 | 198 | 44 | 0 | 1 | 198 | 1 | 198 | 1.42e-93 | 272 |
| MsG0480019603.01.T01 | MTR_4g073070 | 51.869 | 214 | 90 | 3 | 1 | 207 | 3 | 210 | 3.28e-59 | 187 |
| MsG0480019603.01.T01 | MTR_4g094000 | 53.000 | 100 | 46 | 1 | 107 | 206 | 1 | 99 | 7.82e-27 | 100 |
| MsG0480019603.01.T01 | MTR_3g117970 | 33.636 | 110 | 68 | 2 | 80 | 185 | 32 | 140 | 1.77e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019603.01.T01 | AT4G35070 | 31.783 | 129 | 73 | 3 | 78 | 191 | 78 | 206 | 4.89e-12 | 63.9 |
| MsG0480019603.01.T01 | AT4G35070 | 31.783 | 129 | 73 | 3 | 78 | 191 | 23 | 151 | 9.08e-12 | 62.4 |
Find 41 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATAACTTCGCATAACAATT+TGG | 0.247198 | 4:+23164991 | MsG0480019603.01.T01:CDS |
| AGAATTTGAGAAAATACTTA+AGG | 0.382664 | 4:+23164952 | MsG0480019603.01.T01:CDS |
| TGAAACTTGGACATGAGAAT+TGG | 0.392838 | 4:-23164319 | None:intergenic |
| TGTGGTTGTTGTAGTTCAAT+AGG | 0.393104 | 4:-23164243 | None:intergenic |
| GGTAATCATGGATTTCCTTT+CGG | 0.398155 | 4:+23164162 | MsG0480019603.01.T01:CDS |
| GGATTTCCTTTCGGCATCAA+TGG | 0.413294 | 4:+23164171 | MsG0480019603.01.T01:CDS |
| AAATCAGTTCATCAATAATC+AGG | 0.419669 | 4:+23164398 | MsG0480019603.01.T01:CDS |
| AACTTAAGATGCTTGAAACT+TGG | 0.421911 | 4:-23164332 | None:intergenic |
| TGAGAAAATACTTAAGGAAA+GGG | 0.442489 | 4:+23164958 | MsG0480019603.01.T01:CDS |
| AAGAGGAGAAGAAGAAACTA+AGG | 0.448495 | 4:+23165014 | MsG0480019603.01.T01:CDS |
| TATTATGTCGGTGGAATCGC+AGG | 0.448522 | 4:+23164195 | MsG0480019603.01.T01:CDS |
| CCACACTCTCATGGTAATCA+TGG | 0.454463 | 4:+23164150 | MsG0480019603.01.T01:CDS |
| ACAAAATCAACAACAGTGGC+AGG | 0.458608 | 4:+23164278 | MsG0480019603.01.T01:CDS |
| TGTTGTAGTTCAATAGGTTG+TGG | 0.475374 | 4:-23164237 | None:intergenic |
| GGCATCAATGGTTATTATGT+CGG | 0.476149 | 4:+23164183 | MsG0480019603.01.T01:CDS |
| TGCAAAGAAAACACATGAGT+TGG | 0.476231 | 4:+23164895 | MsG0480019603.01.T01:CDS |
| TTGGACATGAGAATTGGAAA+AGG | 0.476885 | 4:-23164313 | None:intergenic |
| GCTTACTTCTTCGCTTTCAC+CGG | 0.495216 | 4:-23165068 | None:intergenic |
| TTGAGAAAATACTTAAGGAA+AGG | 0.501347 | 4:+23164957 | MsG0480019603.01.T01:CDS |
| CACGAAGAAAGATGCCTACA+TGG | 0.502607 | 4:-23165161 | None:intergenic |
| GTGTCTTATTTATGCCATGT+AGG | 0.509532 | 4:+23165147 | MsG0480019603.01.T01:CDS |
| TGCTACTACAACAACATCAA+AGG | 0.512848 | 4:+23164813 | MsG0480019603.01.T01:CDS |
| TTTCTCAAATAGAGAATCAA+AGG | 0.518811 | 4:-23164361 | None:intergenic |
| AATAAGACACCTGAGACACT+TGG | 0.524046 | 4:-23165134 | None:intergenic |
| GCTACTACAACAACATCAAA+GGG | 0.526600 | 4:+23164814 | MsG0480019603.01.T01:CDS |
| AAATCTCGTAAGAAGATTTG+AGG | 0.530617 | 4:+23164919 | MsG0480019603.01.T01:CDS |
| TTCGCATAACAATTTGGAAG+AGG | 0.532491 | 4:+23164997 | MsG0480019603.01.T01:CDS |
| TGGACATGAGAATTGGAAAA+GGG | 0.534112 | 4:-23164312 | None:intergenic |
| ACAAATGATGACAATAAAGA+AGG | 0.554754 | 4:+23164856 | MsG0480019603.01.T01:CDS |
| AATGATGCAAAATCATGTAC+CGG | 0.571497 | 4:+23165049 | MsG0480019603.01.T01:CDS |
| ATCATGCAACCACACTCTCA+TGG | 0.584855 | 4:+23164141 | None:intergenic |
| TCAAATAGAGAATCAAAGGT+AGG | 0.589676 | 4:-23164357 | None:intergenic |
| TCTATTTGAGAAACAAGTCA+AGG | 0.602819 | 4:+23164371 | MsG0480019603.01.T01:CDS |
| ATAAGACACCTGAGACACTT+GGG | 0.606058 | 4:-23165133 | None:intergenic |
| TAATAACCATTGATGCCGAA+AGG | 0.608678 | 4:-23164177 | None:intergenic |
| ATCAATGGTTATTATGTCGG+TGG | 0.625897 | 4:+23164186 | MsG0480019603.01.T01:CDS |
| CAAAATCAACAACAGTGGCA+GGG | 0.642850 | 4:+23164279 | MsG0480019603.01.T01:CDS |
| ATTATGTCGGTGGAATCGCA+GGG | 0.686770 | 4:+23164196 | MsG0480019603.01.T01:CDS |
| AATTGGAAAAGGGAACAACA+TGG | 0.691856 | 4:-23164302 | None:intergenic |
| CCATGATTACCATGAGAGTG+TGG | 0.696444 | 4:-23164150 | None:intergenic |
| AACCACAAAATCAACAACAG+TGG | 0.702825 | 4:+23164274 | MsG0480019603.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAATTTGAGAAAATACTTA+AGG | + | Chr4:23164952-23164971 | MsG0480019603.01.T01:CDS | 20.0% |
| !! | CTACTCAATTAATCTAAAAA+AGG | + | Chr4:23164590-23164609 | MsG0480019603.01.T01:intron | 20.0% |
| !! | TTATTCAATGAACCTAAAAA+AGG | - | Chr4:23164531-23164550 | None:intergenic | 20.0% |
| !!! | TAAGAAAAGATTCCTTTTTT+AGG | + | Chr4:23164516-23164535 | MsG0480019603.01.T01:intron | 20.0% |
| ! | AAATCAGTTCATCAATAATC+AGG | + | Chr4:23164398-23164417 | MsG0480019603.01.T01:CDS | 25.0% |
| ! | AATAACTTCGCATAACAATT+TGG | + | Chr4:23164991-23165010 | MsG0480019603.01.T01:CDS | 25.0% |
| ! | AATTGAGTAGTTAATGTATG+TGG | - | Chr4:23164580-23164599 | None:intergenic | 25.0% |
| ! | ACAAATGATGACAATAAAGA+AGG | + | Chr4:23164856-23164875 | MsG0480019603.01.T01:CDS | 25.0% |
| ! | TGAGAAAATACTTAAGGAAA+GGG | + | Chr4:23164958-23164977 | MsG0480019603.01.T01:CDS | 25.0% |
| ! | TTGAGAAAATACTTAAGGAA+AGG | + | Chr4:23164957-23164976 | MsG0480019603.01.T01:CDS | 25.0% |
| ! | TTTCTCAAATAGAGAATCAA+AGG | - | Chr4:23164364-23164383 | None:intergenic | 25.0% |
| !!! | TCTTTTCTTATATTTGAGAC+AGG | + | Chr4:23164615-23164634 | MsG0480019603.01.T01:intron | 25.0% |
| !!! | TCTTTTCTTATTTTTGAGAC+CGG | - | Chr4:23164506-23164525 | None:intergenic | 25.0% |
| AAATAAAATAGTACTCCCTC+CGG | + | Chr4:23164484-23164503 | MsG0480019603.01.T01:intron | 30.0% | |
| AAATCTCGTAAGAAGATTTG+AGG | + | Chr4:23164919-23164938 | MsG0480019603.01.T01:CDS | 30.0% | |
| AACTTAAGATGCTTGAAACT+TGG | - | Chr4:23164335-23164354 | None:intergenic | 30.0% | |
| AATGATGCAAAATCATGTAC+CGG | + | Chr4:23165049-23165068 | MsG0480019603.01.T01:CDS | 30.0% | |
| TCAAATAGAGAATCAAAGGT+AGG | - | Chr4:23164360-23164379 | None:intergenic | 30.0% | |
| TCTATTTGAGAAACAAGTCA+AGG | + | Chr4:23164371-23164390 | MsG0480019603.01.T01:CDS | 30.0% | |
| ! | TTCTTATATTTGAGACAGGA+GGG | + | Chr4:23164619-23164638 | MsG0480019603.01.T01:intron | 30.0% |
| ! | TTTCTTATATTTGAGACAGG+AGG | + | Chr4:23164618-23164637 | MsG0480019603.01.T01:intron | 30.0% |
| !!! | TTTTGTGGTTGATGATATTG+TGG | - | Chr4:23164264-23164283 | None:intergenic | 30.0% |
| AACCACAAAATCAACAACAG+TGG | + | Chr4:23164274-23164293 | MsG0480019603.01.T01:CDS | 35.0% | |
| AAGAGGAGAAGAAGAAACTA+AGG | + | Chr4:23165014-23165033 | MsG0480019603.01.T01:CDS | 35.0% | |
| AATTGGAAAAGGGAACAACA+TGG | - | Chr4:23164305-23164324 | None:intergenic | 35.0% | |
| ATCAATGGTTATTATGTCGG+TGG | + | Chr4:23164186-23164205 | MsG0480019603.01.T01:CDS | 35.0% | |
| GCTACTACAACAACATCAAA+GGG | + | Chr4:23164814-23164833 | MsG0480019603.01.T01:CDS | 35.0% | |
| GGCATCAATGGTTATTATGT+CGG | + | Chr4:23164183-23164202 | MsG0480019603.01.T01:CDS | 35.0% | |
| GGTAATCATGGATTTCCTTT+CGG | + | Chr4:23164162-23164181 | MsG0480019603.01.T01:CDS | 35.0% | |
| TAATAACCATTGATGCCGAA+AGG | - | Chr4:23164180-23164199 | None:intergenic | 35.0% | |
| TGAAACTTGGACATGAGAAT+TGG | - | Chr4:23164322-23164341 | None:intergenic | 35.0% | |
| TGCAAAGAAAACACATGAGT+TGG | + | Chr4:23164895-23164914 | MsG0480019603.01.T01:CDS | 35.0% | |
| TGCTACTACAACAACATCAA+AGG | + | Chr4:23164813-23164832 | MsG0480019603.01.T01:CDS | 35.0% | |
| TGGACATGAGAATTGGAAAA+GGG | - | Chr4:23164315-23164334 | None:intergenic | 35.0% | |
| TGTGGTTGTTGTAGTTCAAT+AGG | - | Chr4:23164246-23164265 | None:intergenic | 35.0% | |
| TGTTGTAGTTCAATAGGTTG+TGG | - | Chr4:23164240-23164259 | None:intergenic | 35.0% | |
| TTCGCATAACAATTTGGAAG+AGG | + | Chr4:23164997-23165016 | MsG0480019603.01.T01:CDS | 35.0% | |
| TTGGACATGAGAATTGGAAA+AGG | - | Chr4:23164316-23164335 | None:intergenic | 35.0% | |
| !!! | TTCTTATTTTTGAGACCGGA+GGG | - | Chr4:23164502-23164521 | None:intergenic | 35.0% |
| !!! | TTTCTTATTTTTGAGACCGG+AGG | - | Chr4:23164503-23164522 | None:intergenic | 35.0% |
| AATAAGACACCTGAGACACT+TGG | - | Chr4:23165137-23165156 | None:intergenic | 40.0% | |
| ACAAAATCAACAACAGTGGC+AGG | + | Chr4:23164278-23164297 | MsG0480019603.01.T01:CDS | 40.0% | |
| ATAAGACACCTGAGACACTT+GGG | - | Chr4:23165136-23165155 | None:intergenic | 40.0% | |
| CAAAATCAACAACAGTGGCA+GGG | + | Chr4:23164279-23164298 | MsG0480019603.01.T01:CDS | 40.0% | |
| CTTGAAAAACATGTCAGACG+TGG | + | Chr4:23165091-23165110 | MsG0480019603.01.T01:CDS | 40.0% | |
| TCCTCCCAAAGATTCTATCA+CGG | - | Chr4:23164677-23164696 | None:intergenic | 40.0% | |
| ! | CACGTCTGACATGTTTTTCA+AGG | - | Chr4:23165093-23165112 | None:intergenic | 40.0% |
| !!! | TGCCACTGTTGTTGATTTTG+TGG | - | Chr4:23164279-23164298 | None:intergenic | 40.0% |
| ATTATGTCGGTGGAATCGCA+GGG | + | Chr4:23164196-23164215 | MsG0480019603.01.T01:CDS | 45.0% | |
| CCACACTCTCATGGTAATCA+TGG | + | Chr4:23164150-23164169 | MsG0480019603.01.T01:CDS | 45.0% | |
| CCATGATTACCATGAGAGTG+TGG | - | Chr4:23164153-23164172 | None:intergenic | 45.0% | |
| CCTCCCAAAGATTCTATCAC+GGG | - | Chr4:23164676-23164695 | None:intergenic | 45.0% | |
| CTCCCAAAGATTCTATCACG+GGG | - | Chr4:23164675-23164694 | None:intergenic | 45.0% | |
| CTCCCCGTGATAGAATCTTT+GGG | + | Chr4:23164670-23164689 | MsG0480019603.01.T01:intron | 45.0% | |
| GCTTACTTCTTCGCTTTCAC+CGG | - | Chr4:23165071-23165090 | None:intergenic | 45.0% | |
| GGATTTCCTTTCGGCATCAA+TGG | + | Chr4:23164171-23164190 | MsG0480019603.01.T01:CDS | 45.0% | |
| TATTATGTCGGTGGAATCGC+AGG | + | Chr4:23164195-23164214 | MsG0480019603.01.T01:CDS | 45.0% | |
| TCTCCCCGTGATAGAATCTT+TGG | + | Chr4:23164669-23164688 | MsG0480019603.01.T01:intron | 45.0% | |
| ! | TGTTTTGTCCCAAGTGTCTC+AGG | + | Chr4:23165125-23165144 | MsG0480019603.01.T01:CDS | 45.0% |
| CCCGTGATAGAATCTTTGGG+AGG | + | Chr4:23164673-23164692 | MsG0480019603.01.T01:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 23164144 | 23165168 | 23164144 | ID=MsG0480019603.01;Name=MsG0480019603.01 |
| Chr4 | mRNA | 23164144 | 23165168 | 23164144 | ID=MsG0480019603.01.T01;Parent=MsG0480019603.01;Name=MsG0480019603.01.T01;_AED=0.09;_eAED=0.12;_QI=0|0|0|1|1|1|2|0|215 |
| Chr4 | exon | 23164144 | 23164419 | 23164144 | ID=MsG0480019603.01.T01:exon:5096;Parent=MsG0480019603.01.T01 |
| Chr4 | exon | 23164797 | 23165168 | 23164797 | ID=MsG0480019603.01.T01:exon:5097;Parent=MsG0480019603.01.T01 |
| Chr4 | CDS | 23164144 | 23164419 | 23164144 | ID=MsG0480019603.01.T01:cds;Parent=MsG0480019603.01.T01 |
| Chr4 | CDS | 23164797 | 23165168 | 23164797 | ID=MsG0480019603.01.T01:cds;Parent=MsG0480019603.01.T01 |
| Gene Sequence |
| Protein sequence |
