AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380015196.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015196.01.T01 MTR_3g064800 84.667 150 3 1 1 150 1 130 6.29e-86 249
MsG0380015196.01.T01 MTR_3g064800 98.000 100 2 0 51 150 1 100 9.90e-68 202
MsG0380015196.01.T01 MTR_3g064800 97.980 99 2 0 52 150 14 112 4.47e-67 201
MsG0380015196.01.T01 MTR_6g015010 56.098 82 36 0 69 150 366 447 8.26e-25 99.0
MsG0380015196.01.T01 MTR_7g013070 52.439 82 39 0 69 150 337 418 1.80e-21 89.7
MsG0380015196.01.T01 MTR_4g094612 87.500 32 4 0 119 150 1 32 1.18e-14 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380015196.01.T01 AT5G37478 61.905 126 42 2 31 150 14 139 2.65e-47 151
MsG0380015196.01.T01 AT5G37478 66.981 106 29 2 51 150 1 106 2.07e-43 140
MsG0380015196.01.T01 AT5G37478 64.646 99 35 0 52 150 21 119 3.78e-40 132
MsG0380015196.01.T01 AT3G01015 56.410 78 34 0 73 150 366 443 4.43e-22 91.7
MsG0380015196.01.T01 AT5G15510 52.564 78 37 0 73 150 376 453 5.74e-21 88.2
MsG0380015196.01.T01 AT5G15510 52.564 78 37 0 73 150 376 453 5.74e-21 88.2
MsG0380015196.01.T01 AT5G15510 52.564 78 37 0 73 150 376 453 6.07e-21 88.2
MsG0380015196.01.T01 AT5G15510 52.564 78 37 0 73 150 376 453 6.78e-21 88.2
MsG0380015196.01.T01 AT5G37478 55.844 77 28 2 31 101 14 90 2.03e-17 73.9
MsG0380015196.01.T01 AT1G03780 45.205 73 38 2 79 150 645 716 6.26e-12 62.8
MsG0380015196.01.T01 AT1G03780 45.205 73 38 2 79 150 645 716 6.86e-12 62.4

Find 23 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAATGGCTTATCAAAATA+AGG 0.325439 3:+66668284 None:intergenic
AAGCGTTCTTGACAAACTTT+TGG 0.328234 3:+66670088 None:intergenic
CAAAAGAAAGTGAAACAATT+AGG 0.333708 3:+66670133 None:intergenic
AAACACTAAATCTACTTTGA+AGG 0.375816 3:-66670220 MsG0380015196.01.T01:intron
TTGCTCTCTCCATGTTTAAG+TGG 0.437896 3:+66670241 None:intergenic
ATTTACCTTTGTGGGAAGAA+TGG 0.439491 3:+66668269 None:intergenic
AGGAAGAGATACGTTTGCTT+AGG 0.448239 3:-66668337 MsG0380015196.01.T01:CDS
ACGTTTGCTTAGGAAAGAAA+TGG 0.460751 3:-66668327 MsG0380015196.01.T01:CDS
CTTCACAATCTTCATCTCAA+TGG 0.487090 3:-66670068 MsG0380015196.01.T01:CDS
ACGTGCAATGTTCAACTATG+AGG 0.504901 3:-66668598 MsG0380015196.01.T01:intron
CAAAAGATGTACTCAAAGAA+AGG 0.509955 3:-66668793 MsG0380015196.01.T01:CDS
GACAACAAAATTATATCTCA+TGG 0.511192 3:-66668497 MsG0380015196.01.T01:CDS
TGACAGCTCTTTCTTGTGTG+TGG 0.522398 3:+66668622 None:intergenic
ACAAGAGGAGAAGTTGATGA+AGG 0.523831 3:-66668461 MsG0380015196.01.T01:intron
GTGTGGAGTTTGAATTCTTG+TGG 0.533395 3:+66668639 None:intergenic
AAGGTATTATGACAAGACTA+AGG 0.541632 3:-66668774 MsG0380015196.01.T01:intron
TGGAGTAGTACTGATTCTAA+AGG 0.544697 3:-66670048 MsG0380015196.01.T01:CDS
TAAGGCATCAATTGAGCTCG+TGG 0.570757 3:+66668302 None:intergenic
TAGTACTGATTCTAAAGGAA+TGG 0.572965 3:-66670043 MsG0380015196.01.T01:intron
ACATTAATTCCACTTAAACA+TGG 0.573030 3:-66670250 MsG0380015196.01.T01:exon
ATAAGCCATTCTTCCCACAA+AGG 0.601743 3:-66668274 MsG0380015196.01.T01:CDS
TATGGAACAGATGATTGAAG+AGG 0.620275 3:-66668357 MsG0380015196.01.T01:intron
GGAGTTGAGAAAGAGACAAG+AGG 0.644836 3:-66668476 MsG0380015196.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTCAAAATTATTTGAAAAT+AGG + Chr3:66669352-66669371 None:intergenic 10.0%
!!! ATTTTACTAATAATTCAATA+TGG - Chr3:66670154-66670173 MsG0380015196.01.T01:intron 10.0%
!!! TTTTCAAATAATTTTGAAAA+AGG - Chr3:66669353-66669372 MsG0380015196.01.T01:intron 10.0%
!! AATAGTAATAATAGTAAAAG+AGG + Chr3:66669564-66669583 None:intergenic 15.0%
!! ATTATGTTATGTGATAATTA+TGG + Chr3:66669529-66669548 None:intergenic 15.0%
!! TTATGTTATGTGATAATTAT+GGG + Chr3:66669528-66669547 None:intergenic 15.0%
!!! AACTTTTGAAAAGATAAAAT+AGG + Chr3:66669143-66669162 None:intergenic 15.0%
!!! AATTTTAACAAATATGTTAG+AGG + Chr3:66668538-66668557 None:intergenic 15.0%
!!! ATTTTAACAAATATGTTAGA+GGG + Chr3:66668537-66668556 None:intergenic 15.0%
!!! TATAAACTATTTTGAAATAG+CGG + Chr3:66668594-66668613 None:intergenic 15.0%
!!! TTTTAATAAACCAATCATTA+TGG + Chr3:66669645-66669664 None:intergenic 15.0%
!! ATACTTTAGATAGTTGTTAT+AGG - Chr3:66669391-66669410 MsG0380015196.01.T01:intron 20.0%
!! ATCATGATTCATGTATAATA+AGG + Chr3:66669678-66669697 None:intergenic 20.0%
!! ATCATTATGGATAGAATAAA+AGG + Chr3:66669632-66669651 None:intergenic 20.0%
!! CAAAAATGTGAAAAATGAAA+TGG + Chr3:66669823-66669842 None:intergenic 20.0%
!! CATTTCATTTACTATCATTA+AGG + Chr3:66668838-66668857 None:intergenic 20.0%
!! TATGTTATGTGATAATTATG+GGG + Chr3:66669527-66669546 None:intergenic 20.0%
!! TTAGACATGGTATATATTAT+TGG - Chr3:66669457-66669476 MsG0380015196.01.T01:intron 20.0%
!! TTTATTCTATCCATAATGAT+TGG - Chr3:66669632-66669651 MsG0380015196.01.T01:intron 20.0%
!!! CACATTTTTGTGTTTATTTA+GGG - Chr3:66669833-66669852 MsG0380015196.01.T01:intron 20.0%
!!! CTCAAATATTTCTTTTAAGT+TGG - Chr3:66669434-66669453 MsG0380015196.01.T01:intron 20.0%
!!! TCAAATAATTTTGAAAAAGG+TGG - Chr3:66669356-66669375 MsG0380015196.01.T01:intron 20.0%
!!! TCACATTTTTGTGTTTATTT+AGG - Chr3:66669832-66669851 MsG0380015196.01.T01:intron 20.0%
! AAACACTAAATCTACTTTGA+AGG - Chr3:66668309-66668328 MsG0380015196.01.T01:CDS 25.0%
! AAGAATGGCTTATCAAAATA+AGG + Chr3:66670248-66670267 None:intergenic 25.0%
! ACATTAATTCCACTTAAACA+TGG - Chr3:66668279-66668298 MsG0380015196.01.T01:CDS 25.0%
! AGTAAATGAAATGAGATTAG+TGG - Chr3:66668845-66668864 MsG0380015196.01.T01:intron 25.0%
! ATGATTCATGTATAATAAGG+TGG + Chr3:66669675-66669694 None:intergenic 25.0%
! CAAAAGAAAGTGAAACAATT+AGG + Chr3:66668399-66668418 None:intergenic 25.0%
! GACAACAAAATTATATCTCA+TGG - Chr3:66670032-66670051 MsG0380015196.01.T01:intron 25.0%
! GGAATTTGTCTTTCTATATA+TGG + Chr3:66669331-66669350 None:intergenic 25.0%
! GTAAATGAAATGAGATTAGT+GGG - Chr3:66668846-66668865 MsG0380015196.01.T01:intron 25.0%
! TGATATATTGAACCACTATT+TGG - Chr3:66669284-66669303 MsG0380015196.01.T01:intron 25.0%
! TTCATTTATTGATGAATGTG+TGG - Chr3:66669997-66670016 MsG0380015196.01.T01:intron 25.0%
! TTGTGCTTATTTCAAAACAA+AGG + Chr3:66668926-66668945 None:intergenic 25.0%
!! TATGTGTTATTAAAAGCACA+AGG + Chr3:66668980-66668999 None:intergenic 25.0%
!!! AGTTGATGAAGGTATTAATT+AGG - Chr3:66670079-66670098 MsG0380015196.01.T01:CDS 25.0%
!!! GGTTTTGTATTTTCTTTTGT+AGG + Chr3:66669872-66669891 None:intergenic 25.0%
AAAGGACTAGAAACAGATAA+AGG + Chr3:66669614-66669633 None:intergenic 30.0%
ATGACAAGTAGAATATTGCA+TGG + Chr3:66669192-66669211 None:intergenic 30.0%
ATTATGACAAGACTAAGGTA+AGG - Chr3:66669760-66669779 MsG0380015196.01.T01:intron 30.0%
CAAAAGATGTACTCAAAGAA+AGG - Chr3:66669736-66669755 MsG0380015196.01.T01:intron 30.0%
CATAGTACTAATTAACCAAG+AGG + Chr3:66668375-66668394 None:intergenic 30.0%
GCACTTGATCTAAATAATTG+TGG + Chr3:66669484-66669503 None:intergenic 30.0%
TTTGAGTGCAAAATTAAGTG+TGG + Chr3:66669420-66669439 None:intergenic 30.0%
! AAGGTATTATGACAAGACTA+AGG - Chr3:66669755-66669774 MsG0380015196.01.T01:intron 30.0%
! ACTAAATCTACTTTGAAGGT+TGG - Chr3:66668313-66668332 MsG0380015196.01.T01:CDS 30.0%
! ATGAATGTGTGGTTTTTCTT+AGG - Chr3:66670008-66670027 MsG0380015196.01.T01:intron 30.0%
! ATTGCATGGATAAACTTGTT+AGG + Chr3:66669178-66669197 None:intergenic 30.0%
! GCATTTTCCTTAATTTTCCA+TGG - Chr3:66668418-66668437 MsG0380015196.01.T01:intron 30.0%
!! TAGTACTGATTCTAAAGGAA+TGG - Chr3:66668486-66668505 MsG0380015196.01.T01:CDS 30.0%
!!! TAGTTTTTTCTACTTCCTCT+TGG - Chr3:66668357-66668376 MsG0380015196.01.T01:intron 30.0%
!!! TCTTTTAAGTTGGTTAGACA+TGG - Chr3:66669444-66669463 MsG0380015196.01.T01:intron 30.0%
AACACGTACTTACCAAATAG+TGG + Chr3:66669299-66669318 None:intergenic 35.0%
AACTTGCATCACAATGTTGT+TGG - Chr3:66668736-66668755 MsG0380015196.01.T01:intron 35.0%
AATGAAATGAGATTAGTGGG+AGG - Chr3:66668849-66668868 MsG0380015196.01.T01:intron 35.0%
ACAGCATGTATGAAGGATTT+GGG - Chr3:66668706-66668725 MsG0380015196.01.T01:intron 35.0%
ACGTTTGCTTAGGAAAGAAA+TGG - Chr3:66670202-66670221 MsG0380015196.01.T01:intron 35.0%
CTTCACAATCTTCATCTCAA+TGG - Chr3:66668461-66668480 MsG0380015196.01.T01:intron 35.0%
GACTAAGGTAAGGAATTACA+TGG - Chr3:66669770-66669789 MsG0380015196.01.T01:intron 35.0%
GCTATAACGCTATAGCATAA+CGG + Chr3:66668658-66668677 None:intergenic 35.0%
TATGGAACAGATGATTGAAG+AGG - Chr3:66670172-66670191 MsG0380015196.01.T01:intron 35.0%
TTATACTAAACAACGTGTCG+TGG - Chr3:66668609-66668628 MsG0380015196.01.T01:CDS 35.0%
! AAGCGTTCTTGACAAACTTT+TGG + Chr3:66668444-66668463 None:intergenic 35.0%
! CAATAAGCGTCGTTAAATAG+CGG + Chr3:66668682-66668701 None:intergenic 35.0%
! CTTTTGGCCATGGAAAATTA+AGG + Chr3:66668428-66668447 None:intergenic 35.0%
! GTGTTATTAAAAGCACAAGG+TGG + Chr3:66668977-66668996 None:intergenic 35.0%
!! GAGGGTACTGTAAAAAAACT+AGG + Chr3:66668519-66668538 None:intergenic 35.0%
!! TGGAGTAGTACTGATTCTAA+AGG - Chr3:66668481-66668500 MsG0380015196.01.T01:CDS 35.0%
ACGTGCAATGTTCAACTATG+AGG - Chr3:66669931-66669950 MsG0380015196.01.T01:intron 40.0%
AGGAAGAGATACGTTTGCTT+AGG - Chr3:66670192-66670211 MsG0380015196.01.T01:intron 40.0%
ATAAGCCATTCTTCCCACAA+AGG - Chr3:66670255-66670274 MsG0380015196.01.T01:five_prime_UTR 40.0%
CAAATCCTTCATACATGCTG+TGG + Chr3:66668707-66668726 None:intergenic 40.0%
CACAGCATGTATGAAGGATT+TGG - Chr3:66668705-66668724 MsG0380015196.01.T01:intron 40.0%
GTGCATTACCAACAACACTA+TGG - Chr3:66669500-66669519 MsG0380015196.01.T01:intron 40.0%
GTGTGGAGTTTGAATTCTTG+TGG + Chr3:66669893-66669912 None:intergenic 40.0%
TTGCTCTCTCCATGTTTAAG+TGG + Chr3:66668291-66668310 None:intergenic 40.0%
! TCTTGACAAACTTTTGGCCA+TGG + Chr3:66668438-66668457 None:intergenic 40.0%
!! ACAAGAGGAGAAGTTGATGA+AGG - Chr3:66670068-66670087 MsG0380015196.01.T01:CDS 40.0%
!! GCATGGATAAACTTGTTAGG+TGG + Chr3:66669175-66669194 None:intergenic 40.0%
GGAGTTGAGAAAGAGACAAG+AGG - Chr3:66670053-66670072 MsG0380015196.01.T01:CDS 45.0%
TATGGGGTCCATAGTGTTGT+TGG + Chr3:66669511-66669530 None:intergenic 45.0%
TGACAGCTCTTTCTTGTGTG+TGG + Chr3:66669910-66669929 None:intergenic 45.0%
TTGCTCCACAGCATGTATGA+AGG - Chr3:66668699-66668718 MsG0380015196.01.T01:intron 45.0%
! TAAGGCATCAATTGAGCTCG+TGG + Chr3:66670230-66670249 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr3 gene 66668271 66670280 66668271 ID=MsG0380015196.01;Name=MsG0380015196.01
Chr3 mRNA 66668271 66670280 66668271 ID=MsG0380015196.01.T01;Parent=MsG0380015196.01;Name=MsG0380015196.01.T01;_AED=0.44;_eAED=0.51;_QI=27|0.8|0.66|1|0.6|0.66|6|0|150
Chr3 exon 66668271 66668369 66668271 ID=MsG0380015196.01.T01:exon:23071;Parent=MsG0380015196.01.T01
Chr3 exon 66668462 66668521 66668462 ID=MsG0380015196.01.T01:exon:23070;Parent=MsG0380015196.01.T01
Chr3 exon 66668599 66668697 66668599 ID=MsG0380015196.01.T01:exon:23069;Parent=MsG0380015196.01.T01
Chr3 exon 66668775 66668816 66668775 ID=MsG0380015196.01.T01:exon:23068;Parent=MsG0380015196.01.T01
Chr3 exon 66670044 66670163 66670044 ID=MsG0380015196.01.T01:exon:23067;Parent=MsG0380015196.01.T01
Chr3 exon 66670221 66670280 66670221 ID=MsG0380015196.01.T01:exon:23066;Parent=MsG0380015196.01.T01
Chr3 five_prime_UTR 66670254 66670280 66670254 ID=MsG0380015196.01.T01:five_prime_utr;Parent=MsG0380015196.01.T01
Chr3 CDS 66670221 66670253 66670221 ID=MsG0380015196.01.T01:cds;Parent=MsG0380015196.01.T01
Chr3 CDS 66670044 66670163 66670044 ID=MsG0380015196.01.T01:cds;Parent=MsG0380015196.01.T01
Chr3 CDS 66668775 66668816 66668775 ID=MsG0380015196.01.T01:cds;Parent=MsG0380015196.01.T01
Chr3 CDS 66668599 66668697 66668599 ID=MsG0380015196.01.T01:cds;Parent=MsG0380015196.01.T01
Chr3 CDS 66668462 66668521 66668462 ID=MsG0380015196.01.T01:cds;Parent=MsG0380015196.01.T01
Chr3 CDS 66668271 66668369 66668271 ID=MsG0380015196.01.T01:cds;Parent=MsG0380015196.01.T01
Gene Sequence

>MsG0380015196.01.T01

ATGGAGAGAGCAAACACTAAATCTACTTTGAAGTACTATGTCCTAATTGTTTCACTTTCTTTTGCATTTTCCTTAATTTTCCATGGCCAAAAGTTTGTCAAGAACGCTTCACAATCTTCATCTCAATGGAGTAGTACTGATTCTAAAGGAATGACAAAAGATGTACTCAAAGAAAGGTATTATGACAAGACTAAGGGTTCAAGAAAATCTCCTACAAAAGAAAATACAAAACCACAAGAATTCAAACTCCACACACAAGAAAGAGCTGTCAAACGTGCAATGTTCAACTATGAGGTGACAACAAAATTATATCTCATGGAGTTGAGAAAGAGACAAGAGGAGAAGTTGATGAAGATGATTGAAGAGGAAGAGATACGTTTGCTTAGGAAAGAAATGGTTCCACGAGCTCAATTGATGCCTTATTTTGATAAGCCATTCTTCCCACAAAGGTAA

Protein sequence

>MsG0380015196.01.T01

MERANTKSTLKYYVLIVSLSFAFSLIFHGQKFVKNASQSSSQWSSTDSKGMTKDVLKERYYDKTKGSRKSPTKENTKPQEFKLHTQERAVKRAMFNYEVTTKLYLMELRKRQEEKLMKMIEEEEIRLLRKEMVPRAQLMPYFDKPFFPQR*