Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019852.01.T01 | XP_013443444.1 | 97.143 | 385 | 6 | 3 | 1 | 384 | 1 | 381 | 0 | 748 |
| MsG0480019852.01.T02 | XP_013443444.1 | 97.143 | 385 | 6 | 3 | 1 | 384 | 1 | 381 | 0 | 748 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019852.01.T01 | A0A072TIU5 | 97.143 | 385 | 6 | 3 | 1 | 384 | 1 | 381 | 0.0 | 748 |
| MsG0480019852.01.T02 | A0A072TIU5 | 97.143 | 385 | 6 | 3 | 1 | 384 | 1 | 381 | 0.0 | 748 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047910.01 | MsG0480019852.01 | 0.809427 | 1.902529e-50 | 1.116729e-47 |
| MsG0080047967.01 | MsG0480019852.01 | 0.829359 | 5.381013e-55 | 5.495143e-52 |
| MsG0080048059.01 | MsG0480019852.01 | 0.865806 | 4.519902e-65 | 1.489849e-61 |
| MsG0080048697.01 | MsG0480019852.01 | 0.823004 | 1.749303e-53 | 1.487918e-50 |
| MsG0080048776.01 | MsG0480019852.01 | 0.811215 | 7.826444e-51 | 4.816154e-48 |
| MsG0080048800.01 | MsG0480019852.01 | 0.829104 | 6.202823e-55 | 6.286328e-52 |
| MsG0080048896.01 | MsG0480019852.01 | 0.816863 | 4.440450e-52 | 3.183311e-49 |
| MsG0080048917.01 | MsG0480019852.01 | 0.851109 | 1.099143e-60 | 2.200984e-57 |
| MsG0080048919.01 | MsG0480019852.01 | 0.847252 | 1.300858e-59 | 2.298486e-56 |
| MsG0080048929.01 | MsG0480019852.01 | 0.812661 | 3.789760e-51 | 2.423764e-48 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019852.01.T01 | MTR_0013s0150 | 97.143 | 385 | 6 | 3 | 1 | 384 | 1 | 381 | 0.0 | 748 |
| MsG0480019852.01.T01 | MTR_3g033620 | 75.177 | 282 | 68 | 1 | 102 | 383 | 111 | 390 | 7.47e-158 | 449 |
| MsG0480019852.01.T01 | MTR_4g077000 | 72.857 | 280 | 76 | 0 | 101 | 380 | 109 | 388 | 4.89e-153 | 437 |
| MsG0480019852.01.T01 | MTR_3g071970 | 37.288 | 295 | 168 | 6 | 98 | 380 | 427 | 716 | 2.79e-55 | 194 |
| MsG0480019852.01.T01 | MTR_2g084605 | 33.333 | 273 | 169 | 5 | 116 | 380 | 125 | 392 | 6.24e-39 | 143 |
| MsG0480019852.01.T01 | MTR_7g064130 | 31.119 | 286 | 180 | 7 | 105 | 380 | 170 | 448 | 6.29e-32 | 125 |
| MsG0480019852.01.T01 | MTR_7g074090 | 32.479 | 234 | 141 | 5 | 150 | 381 | 90 | 308 | 3.12e-29 | 115 |
| MsG0480019852.01.T01 | MTR_7g074090 | 32.618 | 233 | 140 | 5 | 150 | 380 | 90 | 307 | 1.22e-28 | 115 |
| MsG0480019852.01.T01 | MTR_7g074090 | 32.479 | 234 | 141 | 5 | 150 | 381 | 108 | 326 | 2.40e-28 | 114 |
| MsG0480019852.01.T01 | MTR_7g074070 | 32.645 | 242 | 145 | 5 | 142 | 380 | 92 | 318 | 3.91e-26 | 108 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019852.01.T01 | AT5G12470 | 73.702 | 289 | 76 | 0 | 93 | 381 | 96 | 384 | 2.63e-161 | 458 |
| MsG0480019852.01.T01 | AT3G56140 | 40.000 | 295 | 160 | 6 | 98 | 380 | 200 | 489 | 4.90e-60 | 202 |
| MsG0480019852.01.T01 | AT2G40400 | 40.339 | 295 | 159 | 6 | 98 | 380 | 410 | 699 | 8.23e-60 | 206 |
| MsG0480019852.01.T01 | AT2G40400 | 40.339 | 295 | 159 | 6 | 98 | 380 | 410 | 699 | 8.23e-60 | 206 |
| MsG0480019852.01.T01 | AT3G56140 | 40.000 | 295 | 160 | 6 | 98 | 380 | 419 | 708 | 1.28e-58 | 203 |
| MsG0480019852.01.T01 | AT3G56140 | 40.000 | 295 | 160 | 6 | 98 | 380 | 419 | 708 | 1.28e-58 | 203 |
| MsG0480019852.01.T01 | AT2G40400 | 39.184 | 245 | 133 | 5 | 98 | 330 | 410 | 650 | 2.79e-44 | 162 |
| MsG0480019852.01.T01 | AT5G22790 | 32.542 | 295 | 173 | 8 | 103 | 380 | 148 | 433 | 1.19e-35 | 135 |
| MsG0480019852.01.T01 | AT2G37860 | 31.633 | 294 | 176 | 9 | 103 | 380 | 148 | 432 | 3.09e-33 | 129 |
| MsG0480019852.01.T01 | AT2G37860 | 31.633 | 294 | 176 | 9 | 103 | 380 | 148 | 432 | 3.09e-33 | 129 |
Find 20 sgRNAs with CRISPR-Local
Find 518 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATACAACACACCAACATAA+TGG | 0.375327 | 4:+28485958 | None:intergenic |
| AGAGAAACAAAGTGAAGAAA+AGG | 0.424593 | 4:+28485664 | None:intergenic |
| AGTCCTGCTAGCTATGTTGA+TGG | 0.451552 | 4:+28485841 | None:intergenic |
| ATTGTGTCATGAAATCAATC+GGG | 0.588382 | 4:-28486016 | MsG0480019852.01.T01:three_prime_UTR |
| CCACGCATAGTGTCCAGCGA+AGG | 0.656661 | 4:-28485872 | MsG0480019852.01.T01:three_prime_UTR |
| CCTTCGCTGGACACTATGCG+TGG | 0.667915 | 4:+28485872 | None:intergenic |
| GATCCATCAACATAGCTAGC+AGG | 0.558269 | 4:-28485844 | MsG0480019852.01.T01:three_prime_UTR |
| GATGGATCTTTAACCTTCGC+TGG | 0.430139 | 4:+28485859 | None:intergenic |
| GGGTTGGAGTCCAGAAAATA+AGG | 0.297126 | 4:-28486064 | MsG0480019852.01.T01:CDS |
| GGTGGGTGGATTATGCTCGC+TGG | 0.481668 | 4:-28486085 | MsG0480019852.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATCAAGTGTGTTTCGA+TGG | - | Chr4:28488191-28488210 | MsG0480019852.01.T01:intron | 30.0% | |
| AACTATATAGTAGGACTGTA+GGG | - | Chr4:28491220-28491239 | MsG0480019852.01.T01:intron | 30.0% | |
| AAGATAGAGATGAATTCACA+TGG | + | Chr4:28486534-28486553 | None:intergenic | 30.0% | |
| AAGCATAAAATCTGCTACAA+TGG | + | Chr4:28490529-28490548 | None:intergenic | 30.0% | |
| AATACAACACACCAACATAA+TGG | + | Chr4:28492249-28492268 | None:intergenic | 30.0% | |
| AATTAGACTTGTATTGCCAA+TGG | - | Chr4:28488847-28488866 | MsG0480019852.01.T01:intron | 30.0% | |
| ACATGAATCTTACCATGAAT+AGG | - | Chr4:28492098-28492117 | MsG0480019852.01.T01:intron | 30.0% | |
| ACGTGACAAAAACAAAACTA+AGG | + | Chr4:28492569-28492588 | None:intergenic | 30.0% | |
| AGAGAAACAAAGTGAAGAAA+AGG | + | Chr4:28492543-28492562 | None:intergenic | 30.0% | |
| AGATGAATTCACATGGAAAA+CGG | + | Chr4:28486527-28486546 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 28485582 | 28492644 | 28485582 | ID=MsG0480019852.01;Name=MsG0480019852.01 |
| Chr4 | mRNA | 28485693 | 28492644 | 28485693 | ID=MsG0480019852.01.T01;Parent=MsG0480019852.01;Name=MsG0480019852.01.T01;_AED=0.34;_eAED=0.34;_QI=145|1|1|1|0.77|0.6|10|342|384 |
| Chr4 | exon | 28485693 | 28485767 | 28485693 | ID=MsG0480019852.01.T01:exon:9378;Parent=MsG0480019852.01.T01 |
| Chr4 | exon | 28485791 | 28486106 | 28485791 | ID=MsG0480019852.01.T01:exon:9377;Parent=MsG0480019852.01.T01 |
| Chr4 | five_prime_UTR | 28492616 | 28492644 | 28492616 | ID=MsG0480019852.01.T01:five_prime_utr;Parent=MsG0480019852.01.T01 |
| Chr4 | five_prime_UTR | 28490457 | 28490572 | 28490457 | ID=MsG0480019852.01.T01:five_prime_utr;Parent=MsG0480019852.01.T01 |
| Chr4 | CDS | 28489917 | 28490456 | 28489917 | ID=MsG0480019852.01.T01:cds;Parent=MsG0480019852.01.T01 |
| Chr4 | CDS | 28487816 | 28487884 | 28487816 | ID=MsG0480019852.01.T01:cds;Parent=MsG0480019852.01.T01 |
| Chr4 | CDS | 28487576 | 28487707 | 28487576 | ID=MsG0480019852.01.T01:cds;Parent=MsG0480019852.01.T01 |
| Chr4 | CDS | 28487410 | 28487463 | 28487410 | ID=MsG0480019852.01.T01:cds;Parent=MsG0480019852.01.T01 |
| Chr4 | CDS | 28487282 | 28487326 | 28487282 | ID=MsG0480019852.01.T01:cds;Parent=MsG0480019852.01.T01 |
| Chr4 | CDS | 28486425 | 28486564 | 28486425 | ID=MsG0480019852.01.T01:cds;Parent=MsG0480019852.01.T01 |
| Chr4 | CDS | 28486204 | 28486329 | 28486204 | ID=MsG0480019852.01.T01:cds;Parent=MsG0480019852.01.T01 |
| Chr4 | CDS | 28486058 | 28486106 | 28486058 | ID=MsG0480019852.01.T01:cds;Parent=MsG0480019852.01.T01 |
| Chr4 | three_prime_UTR | 28485791 | 28486057 | 28485791 | ID=MsG0480019852.01.T01:three_prime_utr;Parent=MsG0480019852.01.T01 |
| Chr4 | three_prime_UTR | 28485693 | 28485767 | 28485693 | ID=MsG0480019852.01.T01:three_prime_utr;Parent=MsG0480019852.01.T01 |
| Chr4 | mRNA | 28485582 | 28492644 | 28485582 | ID=MsG0480019852.01.T02;Parent=MsG0480019852.01;Name=MsG0480019852.01.T02;_AED=0.32;_eAED=0.32;_QI=145|1|1|1|0.87|0.77|9|476|384 |
| Chr4 | exon | 28485582 | 28486106 | 28485582 | ID=MsG0480019852.01.T02:exon:9379;Parent=MsG0480019852.01.T02 |
| Chr4 | five_prime_UTR | 28492616 | 28492644 | 28492616 | ID=MsG0480019852.01.T02:five_prime_utr;Parent=MsG0480019852.01.T02 |
| Chr4 | five_prime_UTR | 28490457 | 28490572 | 28490457 | ID=MsG0480019852.01.T02:five_prime_utr;Parent=MsG0480019852.01.T02 |
| Chr4 | CDS | 28489917 | 28490456 | 28489917 | ID=MsG0480019852.01.T02:cds;Parent=MsG0480019852.01.T02 |
| Chr4 | CDS | 28487816 | 28487884 | 28487816 | ID=MsG0480019852.01.T02:cds;Parent=MsG0480019852.01.T02 |
| Chr4 | CDS | 28487576 | 28487707 | 28487576 | ID=MsG0480019852.01.T02:cds;Parent=MsG0480019852.01.T02 |
| Chr4 | CDS | 28487410 | 28487463 | 28487410 | ID=MsG0480019852.01.T02:cds;Parent=MsG0480019852.01.T02 |
| Chr4 | CDS | 28487282 | 28487326 | 28487282 | ID=MsG0480019852.01.T02:cds;Parent=MsG0480019852.01.T02 |
| Chr4 | CDS | 28486425 | 28486564 | 28486425 | ID=MsG0480019852.01.T02:cds;Parent=MsG0480019852.01.T02 |
| Chr4 | CDS | 28486204 | 28486329 | 28486204 | ID=MsG0480019852.01.T02:cds;Parent=MsG0480019852.01.T02 |
| Chr4 | CDS | 28486058 | 28486106 | 28486058 | ID=MsG0480019852.01.T02:cds;Parent=MsG0480019852.01.T02 |
| Chr4 | three_prime_UTR | 28485582 | 28486057 | 28485582 | ID=MsG0480019852.01.T02:three_prime_utr;Parent=MsG0480019852.01.T02 |
| Gene Sequence |
>MsG0480019852.01.T02 ATGGCCATGGCTTCTTCATCATCATTCTCCACCATCCACCCTCGTTCTTTCTCTCTCTCCACCCAAAACCACCACTCTTCTTCAACCTCTCTCCCACTCCCATCTCTCTCCATCATCTCCCGCCGTCGTCCACATTCTCATTCCAACAACCTCTTCACTCCCCGTCCTCCCATAACCGCCATTTATGCATCCACCAACGGTGGCGGCGGAATTGGAGAATCAGGTGGCAGTGGAAGCGGTGGAGGAGGTGATGGTGGTGGCGATGATGGTGAATCAGAGGATAGAGATCGAAACAGAGAAGAAGCGTTGCTGGTACTTACGGAAGTAGGTAAGTCATTGGAGAATTTGCCAGCGGATTTAGCCGAAGCGGTGAAAGCGGGACGAGTTCCGGGTTCGATTGTGAAGAGATTCTTTGAGCTGCAAAAATCAAGTGTGTTTCGATGGTTGCTTAATTTTGGTGGGTTTAGGGAAAGATTGCTTGCTGATGATTTGTTTCTTGCTAAGGTTGCTATGGAATGCGGTGTTGGCATCTTTACAAAGACTGCTGCGGAGTTGGAGAAGCGAAAAGAAAACTTTACTAAAGAACTTGATTTTGTCTGCGCTGATGTGGTAATGGCCATTGTAGCAGATTTTATGCTTGTGTGGCTTCCTGCTCCGACGGTTTCTCTTCGACCACCACCTTCCGTCAGTGCTGGACTTATTGCTAAATTCTTCTTCGGCTGCCCTGAAAATGCTTTTCAGGTGGCTTTGGCTGGAACGTCATATTCACTTATACAGAGAATAGGTGCTATTGTGCGGAACGGAGCTAAGCTATTTGCAGTTGGGACTGGTGCATCACTGATTGGTACTGGTGTAACAAACGCATTGATTAATGCGCGGAAGGTTGTTGATAAATCTTTTGCTGACGAGGCAGAGGAAGTACCGGTATTATCAACGAGTGTTGCCTATGGGGTTTACATGGCAGTATCTAGCAACTTGAGGTATCAAATTCTAGCTGGAATTATTGAACAACGGATTTTAGATCCTTTGCTGCACCAAAACAAGCTCATGTTAAGTGCAATCTGCTTTGCTGTTAGAACCGGAAACACTTTCTTGGGCTCGCTATTGTGGGTGGATTATGCTCGCTGGGTTGGAGTCCAGAAAATAAGGGATTAA >MsG0480019852.01.T01 ATGGCCATGGCTTCTTCATCATCATTCTCCACCATCCACCCTCGTTCTTTCTCTCTCTCCACCCAAAACCACCACTCTTCTTCAACCTCTCTCCCACTCCCATCTCTCTCCATCATCTCCCGCCGTCGTCCACATTCTCATTCCAACAACCTCTTCACTCCCCGTCCTCCCATAACCGCCATTTATGCATCCACCAACGGTGGCGGCGGAATTGGAGAATCAGGTGGCAGTGGAAGCGGTGGAGGAGGTGATGGTGGTGGCGATGATGGTGAATCAGAGGATAGAGATCGAAACAGAGAAGAAGCGTTGCTGGTACTTACGGAAGTAGGTAAGTCATTGGAGAATTTGCCAGCGGATTTAGCCGAAGCGGTGAAAGCGGGACGAGTTCCGGGTTCGATTGTGAAGAGATTCTTTGAGCTGCAAAAATCAAGTGTGTTTCGATGGTTGCTTAATTTTGGTGGGTTTAGGGAAAGATTGCTTGCTGATGATTTGTTTCTTGCTAAGGTTGCTATGGAATGCGGTGTTGGCATCTTTACAAAGACTGCTGCGGAGTTGGAGAAGCGAAAAGAAAACTTTACTAAAGAACTTGATTTTGTCTGCGCTGATGTGGTAATGGCCATTGTAGCAGATTTTATGCTTGTGTGGCTTCCTGCTCCGACGGTTTCTCTTCGACCACCACCTTCCGTCAGTGCTGGACTTATTGCTAAATTCTTCTTCGGCTGCCCTGAAAATGCTTTTCAGGTGGCTTTGGCTGGAACGTCATATTCACTTATACAGAGAATAGGTGCTATTGTGCGGAACGGAGCTAAGCTATTTGCAGTTGGGACTGGTGCATCACTGATTGGTACTGGTGTAACAAACGCATTGATTAATGCGCGGAAGGTTGTTGATAAATCTTTTGCTGACGAGGCAGAGGAAGTACCGGTATTATCAACGAGTGTTGCCTATGGGGTTTACATGGCAGTATCTAGCAACTTGAGGTATCAAATTCTAGCTGGAATTATTGAACAACGGATTTTAGATCCTTTGCTGCACCAAAACAAGCTCATGTTAAGTGCAATCTGCTTTGCTGTTAGAACCGGAAACACTTTCTTGGGCTCGCTATTGTGGGTGGATTATGCTCGCTGGGTTGGAGTCCAGAAAATAAGGGATTAA |
| Protein sequence |
>MsG0480019852.01.T02 MAMASSSSFSTIHPRSFSLSTQNHHSSSTSLPLPSLSIISRRRPHSHSNNLFTPRPPITAIYASTNGGGGIGESGGSGSGGGGDGGGDDGESEDRDRNREEALLVLTEVGKSLENLPADLAEAVKAGRVPGSIVKRFFELQKSSVFRWLLNFGGFRERLLADDLFLAKVAMECGVGIFTKTAAELEKRKENFTKELDFVCADVVMAIVADFMLVWLPAPTVSLRPPPSVSAGLIAKFFFGCPENAFQVALAGTSYSLIQRIGAIVRNGAKLFAVGTGASLIGTGVTNALINARKVVDKSFADEAEEVPVLSTSVAYGVYMAVSSNLRYQILAGIIEQRILDPLLHQNKLMLSAICFAVRTGNTFLGSLLWVDYARWVGVQKIRD* >MsG0480019852.01.T01 MAMASSSSFSTIHPRSFSLSTQNHHSSSTSLPLPSLSIISRRRPHSHSNNLFTPRPPITAIYASTNGGGGIGESGGSGSGGGGDGGGDDGESEDRDRNREEALLVLTEVGKSLENLPADLAEAVKAGRVPGSIVKRFFELQKSSVFRWLLNFGGFRERLLADDLFLAKVAMECGVGIFTKTAAELEKRKENFTKELDFVCADVVMAIVADFMLVWLPAPTVSLRPPPSVSAGLIAKFFFGCPENAFQVALAGTSYSLIQRIGAIVRNGAKLFAVGTGASLIGTGVTNALINARKVVDKSFADEAEEVPVLSTSVAYGVYMAVSSNLRYQILAGIIEQRILDPLLHQNKLMLSAICFAVRTGNTFLGSLLWVDYARWVGVQKIRD* |
