AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020317.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020317.01.T01 MTR_4g047610 97.890 237 5 0 1 237 107 343 7.55e-176 487
MsG0480020317.01.T01 MTR_5g022560 94.515 237 13 0 1 237 108 344 1.59e-170 473
MsG0480020317.01.T01 MTR_4g079470 93.671 237 14 1 1 237 110 345 2.04e-167 466
MsG0480020317.01.T01 MTR_4g079770 93.671 237 14 1 1 237 110 345 2.30e-167 465
MsG0480020317.01.T01 MTR_4g107930 92.827 237 17 0 1 237 109 345 4.15e-167 465
MsG0480020317.01.T01 MTR_4g079800 89.873 237 23 1 1 237 110 345 1.16e-160 448
MsG0480020317.01.T01 MTR_2g075830 82.096 229 41 0 9 237 113 341 1.75e-136 387
MsG0480020317.01.T01 MTR_4g080700 70.870 230 65 2 8 237 113 340 4.35e-122 350
MsG0480020317.01.T01 MTR_4g080730 70.870 230 65 2 8 237 113 340 1.05e-119 344
MsG0480020317.01.T01 MTR_4g104370 68.444 225 69 2 11 235 120 342 2.92e-113 329
MsG0480020317.01.T01 MTR_3g075390 62.712 236 86 2 1 235 109 343 7.06e-108 315
MsG0480020317.01.T01 MTR_2g083930 59.657 233 94 0 3 235 110 342 1.94e-105 308
MsG0480020317.01.T01 MTR_7g078600 63.274 226 78 3 11 235 115 336 1.70e-104 306
MsG0480020317.01.T01 MTR_4g080360 58.798 233 95 1 5 237 111 342 2.78e-104 305
MsG0480020317.01.T01 MTR_4g079440 62.667 225 83 1 11 235 120 343 3.89e-104 305
MsG0480020317.01.T01 MTR_3g116080 63.274 226 80 3 11 236 125 347 9.97e-101 296
MsG0480020317.01.T01 MTR_7g079230 60.870 230 87 2 6 235 108 334 5.74e-100 294
MsG0480020317.01.T01 MTR_2g090890 58.772 228 93 1 9 236 110 336 3.71e-99 292
MsG0480020317.01.T01 MTR_1g107550 60.515 233 89 3 4 235 124 354 7.18e-99 296
MsG0480020317.01.T01 MTR_4g094918 56.962 237 99 3 1 236 107 341 7.45e-98 290
MsG0480020317.01.T01 MTR_3g102220 58.475 236 95 3 1 235 127 360 1.75e-95 287
MsG0480020317.01.T01 MTR_1g018840 58.442 231 93 3 6 235 132 360 9.99e-94 282
MsG0480020317.01.T01 MTR_1g018840 58.442 231 93 3 6 235 132 360 1.53e-93 282
MsG0480020317.01.T01 MTR_3g112430 58.257 218 89 2 19 236 126 341 1.46e-86 263
MsG0480020317.01.T01 MTR_2g015850 54.894 235 102 3 1 235 132 362 2.55e-81 248
MsG0480020317.01.T01 MTR_2g016270 53.070 228 100 4 12 236 117 340 3.52e-75 235
MsG0480020317.01.T01 MTR_2g016180 53.070 228 100 4 12 236 132 355 4.09e-75 236
MsG0480020317.01.T01 MTR_7g078630 53.960 202 77 4 6 196 754 950 1.33e-63 213
MsG0480020317.01.T01 MTR_1g023810 47.489 219 106 6 19 234 49 261 3.78e-63 198
MsG0480020317.01.T01 MTR_4g065077 44.493 227 106 6 19 236 141 356 7.84e-58 187
MsG0480020317.01.T01 MTR_8g086470 44.493 227 106 6 19 236 141 356 8.18e-58 187
MsG0480020317.01.T01 MTR_2g015920 45.946 222 110 7 20 236 142 358 9.57e-58 187
MsG0480020317.01.T01 MTR_4g125300 44.843 223 111 6 19 236 133 348 1.45e-56 184
MsG0480020317.01.T01 MTR_1g023210 43.860 228 100 7 19 228 135 352 2.52e-56 184
MsG0480020317.01.T01 MTR_4g071060 43.478 230 108 7 19 233 131 353 2.19e-55 181
MsG0480020317.01.T01 MTR_2g067900 47.107 242 111 9 3 234 89 323 1.40e-53 176
MsG0480020317.01.T01 MTR_2g015930 45.133 226 111 8 17 236 140 358 8.55e-52 172
MsG0480020317.01.T01 MTR_2g067560 48.402 219 97 10 19 231 116 324 9.89e-51 177
MsG0480020317.01.T01 MTR_2g064475 44.978 229 103 12 19 236 135 351 1.31e-50 168
MsG0480020317.01.T01 MTR_1g094135 40.506 237 113 7 15 233 137 363 5.69e-50 167
MsG0480020317.01.T01 MTR_2g066980 37.500 224 95 8 19 236 134 318 7.17e-42 145
MsG0480020317.01.T01 MTR_7g078580 63.265 98 35 1 138 235 4 100 8.17e-42 139
MsG0480020317.01.T01 MTR_7g079130 68.889 90 28 0 146 235 7 96 6.14e-41 135
MsG0480020317.01.T01 MTR_2g015840 56.250 112 48 1 125 236 161 271 5.38e-37 131
MsG0480020317.01.T01 MTR_2g015840 51.852 54 26 0 1 54 103 156 1.55e-12 65.9
MsG0480020317.01.T01 MTR_4g072790 38.318 214 96 7 19 228 131 312 1.94e-35 128
MsG0480020317.01.T01 MTR_1g025450 41.530 183 80 8 13 189 47 208 2.55e-34 129
MsG0480020317.01.T01 MTR_4g073660 36.697 218 98 7 19 228 130 315 4.85e-34 125
MsG0480020317.01.T01 MTR_4g072340 36.697 218 98 7 19 228 131 316 4.89e-34 125
MsG0480020317.01.T01 MTR_7g078310 49.541 109 53 1 6 112 139 247 1.23e-28 109
MsG0480020317.01.T01 MTR_2g016380 46.667 120 59 3 120 236 18 135 4.41e-28 107
MsG0480020317.01.T01 MTR_7g111060 28.112 249 147 8 5 228 89 330 4.70e-24 99.0
MsG0480020317.01.T01 MTR_7g079260 59.722 72 13 1 162 233 7 62 6.00e-23 90.1
MsG0480020317.01.T01 MTR_7g111050 27.969 261 154 9 3 236 84 337 1.02e-22 95.1
MsG0480020317.01.T01 MTR_3g029560 28.302 212 122 6 31 236 50 237 3.53e-22 91.7
MsG0480020317.01.T01 MTR_8g087510 41.221 131 70 5 60 188 23 148 8.93e-21 86.3
MsG0480020317.01.T01 MTR_3g082370 26.570 207 132 5 21 212 2 203 7.68e-19 82.4
MsG0480020317.01.T01 MTR_2g047960 48.235 85 43 1 28 112 7 90 1.66e-18 78.2
MsG0480020317.01.T01 MTR_7g111050 25.792 221 136 7 3 198 79 296 9.90e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020317.01.T01 AT5G50260 66.667 228 75 1 8 235 115 341 8.64e-113 328
MsG0480020317.01.T01 AT5G45890 65.532 235 77 2 4 236 113 345 2.66e-112 326
MsG0480020317.01.T01 AT3G48340 66.087 230 76 2 6 235 115 342 1.83e-110 322
MsG0480020317.01.T01 AT3G48350 62.555 227 83 2 11 236 118 343 1.99e-101 299
MsG0480020317.01.T01 AT4G35350 60.699 229 88 2 8 236 126 352 3.20e-100 295
MsG0480020317.01.T01 AT5G43060 64.679 218 75 2 18 235 137 352 5.69e-99 296
MsG0480020317.01.T01 AT3G19390 60.793 227 86 3 11 236 121 345 3.69e-98 293
MsG0480020317.01.T01 AT4G36880 61.712 222 82 3 15 235 141 360 1.15e-97 290
MsG0480020317.01.T01 AT1G06260 63.182 220 79 2 16 235 124 341 4.25e-97 287
MsG0480020317.01.T01 AT1G20850 59.211 228 91 2 9 236 128 353 9.29e-97 287
MsG0480020317.01.T01 AT3G19400 57.082 233 97 3 6 236 117 348 1.24e-95 284
MsG0480020317.01.T01 AT1G47128 58.009 231 93 3 7 235 123 351 2.55e-95 286
MsG0480020317.01.T01 AT2G27420 55.285 246 97 3 4 237 104 348 1.46e-93 278
MsG0480020317.01.T01 AT3G49340 56.277 231 97 2 7 237 115 341 3.37e-93 277
MsG0480020317.01.T01 AT2G34080 57.078 219 91 2 19 237 130 345 8.66e-91 271
MsG0480020317.01.T01 AT4G23520 57.078 219 89 4 19 235 133 348 4.71e-89 267
MsG0480020317.01.T01 AT1G09850 58.525 217 88 2 19 235 118 332 7.98e-88 267
MsG0480020317.01.T01 AT4G11310 52.155 232 107 3 5 235 123 351 3.96e-86 260
MsG0480020317.01.T01 AT1G29090 56.279 215 90 3 24 237 144 355 3.26e-84 255
MsG0480020317.01.T01 AT4G11320 50.431 232 111 3 5 235 130 358 7.30e-84 254
MsG0480020317.01.T01 AT4G11320 50.431 232 111 3 5 235 130 358 7.30e-84 254
MsG0480020317.01.T01 AT1G29080 54.419 215 94 3 24 237 135 346 4.15e-82 249
MsG0480020317.01.T01 AT3G43960 48.472 229 112 5 11 235 119 345 5.17e-69 216
MsG0480020317.01.T01 AT4G35350 60.123 163 63 2 8 170 126 286 7.81e-67 208
MsG0480020317.01.T01 AT3G48350 61.491 161 60 2 11 170 118 277 3.03e-63 199
MsG0480020317.01.T01 AT3G19400 56.287 167 71 2 6 170 117 283 6.13e-62 196
MsG0480020317.01.T01 AT1G29110 45.833 216 101 4 22 236 133 333 9.76e-62 197
MsG0480020317.01.T01 AT5G60360 46.222 225 109 7 17 236 139 356 1.00e-57 187
MsG0480020317.01.T01 AT3G45310 44.248 226 114 7 17 237 139 357 1.21e-55 182
MsG0480020317.01.T01 AT5G60360 45.778 225 109 8 17 236 139 355 3.60e-55 181
MsG0480020317.01.T01 AT2G21430 43.111 225 106 7 19 228 132 349 6.74e-55 180
MsG0480020317.01.T01 AT4G39090 44.000 225 104 7 19 228 135 352 1.60e-53 176
MsG0480020317.01.T01 AT5G60360 45.116 215 110 6 17 226 139 350 1.96e-53 176
MsG0480020317.01.T01 AT3G45310 43.363 226 115 7 17 237 139 356 3.26e-53 175
MsG0480020317.01.T01 AT3G54940 42.105 228 104 7 19 228 137 354 1.07e-51 172
MsG0480020317.01.T01 AT4G16190 41.152 243 122 7 6 233 127 363 5.18e-50 167
MsG0480020317.01.T01 AT3G54940 43.972 141 71 2 19 152 137 276 7.96e-36 128
MsG0480020317.01.T01 AT1G02305 29.362 235 134 8 19 228 106 333 2.11e-24 99.8
MsG0480020317.01.T01 AT4G01610 28.631 241 140 8 13 228 97 330 1.27e-22 95.1
MsG0480020317.01.T01 AT4G01610 28.216 241 141 8 13 228 97 330 2.42e-21 91.7
MsG0480020317.01.T01 AT1G02300 29.717 212 121 7 38 228 146 350 2.95e-20 88.6

Find 94 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTTGCATTGTTTCTATGTT+TGG 0.177307 4:-38828549 MsG0480020317.01.T01:five_prime_UTR
TTTGCATTGTTTCTATGTTT+GGG 0.258152 4:-38828548 MsG0480020317.01.T01:five_prime_UTR
TGATAAAGAGATCAACTTTC+CGG 0.269436 4:+38827995 None:intergenic
AACACCTTGATAAGGGTAAT+TGG 0.282835 4:+38827860 None:intergenic
ATCAATGCAGTTACCATTAC+TGG 0.289989 4:-38827807 MsG0480020317.01.T01:CDS
TCACTATGGCAAAGTTTATA+AGG 0.293530 4:-38828450 MsG0480020317.01.T01:five_prime_UTR
AATATGATATAAGTGATTAT+TGG 0.319024 4:+38828574 None:intergenic
GTTATTGTCACCTTTGTTGA+AGG 0.327578 4:+38828367 None:intergenic
AGGTTGTGAGGGTGGTCTTA+TGG 0.350865 4:-38827931 MsG0480020317.01.T01:CDS
AAATTCATCATTCAAAATCA+TGG 0.364764 4:-38827897 MsG0480020317.01.T01:CDS
GAACTCTTGGGGAACCGATT+GGG 0.373659 4:-38827574 MsG0480020317.01.T01:CDS
TAGTTTGAATTACAAAGTTT+AGG 0.374546 4:+38827435 None:intergenic
ATTGGTTGGTTAAGAACTCT+TGG 0.408910 4:-38827587 MsG0480020317.01.T01:CDS
AGTAATGGTAACTGCATTGA+TGG 0.411113 4:+38827809 None:intergenic
CAGCAATGGATGGGTCACTA+TGG 0.420749 4:-38828464 MsG0480020317.01.T01:five_prime_UTR
CATAGTGACCCATCCATTGC+TGG 0.428097 4:+38828465 None:intergenic
TTGGTTGGTTAAGAACTCTT+GGG 0.431188 4:-38827586 MsG0480020317.01.T01:CDS
GCATCAATGGCTACAGAAAT+CGG 0.431804 4:+38827726 None:intergenic
GGTGTTACCGCTGTGGGTTA+TGG 0.435442 4:-38827633 MsG0480020317.01.T01:CDS
AGTCAATTTATGAATTCCTT+CGG 0.437717 4:+38828019 None:intergenic
GGCTACAGAAATCGGTTGAT+TGG 0.443785 4:+38827734 None:intergenic
TAAATTGAAGCCTTCAACAA+AGG 0.453792 4:-38828377 MsG0480020317.01.T01:five_prime_UTR
GACTTTCAATTCTACAAAAG+TGG 0.454514 4:-38827693 MsG0480020317.01.T01:CDS
TTCCGTCAACACCTTGATAA+GGG 0.456935 4:+38827853 None:intergenic
AATGCCTGCTCATTGTTAGC+AGG 0.459083 4:+38827771 None:intergenic
GTTCCGTCAACACCTTGATA+AGG 0.464383 4:+38827852 None:intergenic
TTGATAAGGGTAATTGGCTT+CGG 0.466155 4:+38827866 None:intergenic
CAATTCCATCCTCGGTGGAT+TGG 0.467247 4:-38828208 MsG0480020317.01.T01:CDS
CATTGTCCTTGGTTCTTCAC+CGG 0.468809 4:+38828164 None:intergenic
AACACGTACCACATTGTCCT+TGG 0.469044 4:+38828153 None:intergenic
TACCCTTATCAAGGTGTTGA+CGG 0.470988 4:-38827855 MsG0480020317.01.T01:CDS
TTTATAAGGATTCTCAAGAA+AGG 0.473803 4:-38828436 MsG0480020317.01.T01:five_prime_UTR
TCTCATACATGGAGTCATCT+TGG 0.474251 4:+38828492 None:intergenic
TTGCTGGTGCCTCTCATACA+TGG 0.480269 4:+38828481 None:intergenic
GTCGGAACCACTAGCATCAA+TGG 0.480791 4:+38827713 None:intergenic
TTCCTCCAATCCACCGAGGA+TGG 0.482112 4:+38828203 None:intergenic
TTATAAGGATTCTCAAGAAA+GGG 0.488285 4:-38828435 MsG0480020317.01.T01:five_prime_UTR
GTATCCTTCTTCACCCCAAT+CGG 0.492451 4:+38827560 None:intergenic
TGGATCATGCGGAACTGAGT+TGG 0.493619 4:-38827661 MsG0480020317.01.T01:CDS
AGAACTCTTGGGGAACCGAT+TGG 0.493995 4:-38827575 MsG0480020317.01.T01:CDS
TGCAAGTGCAATTCCATCCT+CGG 0.502103 4:-38828216 MsG0480020317.01.T01:CDS
AACATCTAGAAACAAATTCA+AGG 0.503470 4:-38828288 MsG0480020317.01.T01:five_prime_UTR
TTCCATCCTCGGTGGATTGG+AGG 0.506186 4:-38828205 MsG0480020317.01.T01:CDS
TCTGTAGCCATTGATGCTAG+TGG 0.514851 4:-38827720 MsG0480020317.01.T01:CDS
GTAACGATGGAACTAAGTAT+TGG 0.515684 4:-38827605 MsG0480020317.01.T01:CDS
GGAACCGATTGGGGTGAAGA+AGG 0.521876 4:-38827564 MsG0480020317.01.T01:CDS
AACTCTTGGGGAACCGATTG+GGG 0.523168 4:-38827573 MsG0480020317.01.T01:CDS
TGAGAGGCACCAGCAATGGA+TGG 0.535269 4:-38828474 MsG0480020317.01.T01:five_prime_UTR
GGAATTCATAAATTGACTAC+CGG 0.539888 4:-38828014 MsG0480020317.01.T01:CDS
GTGGACCAAGGTTGTGAGGG+TGG 0.540762 4:-38827939 MsG0480020317.01.T01:CDS
TCGGTGGATTGGAGGAAGAA+AGG 0.541518 4:-38828197 MsG0480020317.01.T01:CDS
ACTAGGCATTAATCAATTTG+CGG 0.545109 4:-38828333 MsG0480020317.01.T01:five_prime_UTR
TAATTCAAACTAAATTGTGA+TGG 0.548968 4:-38827424 MsG0480020317.01.T01:three_prime_UTR
ACATCTAGAAACAAATTCAA+GGG 0.549047 4:-38828287 MsG0480020317.01.T01:five_prime_UTR
CGATGGAACTAAGTATTGGT+TGG 0.551216 4:-38827601 MsG0480020317.01.T01:CDS
AGAAATCGGTTGATTGGCCA+CGG 0.552499 4:+38827740 None:intergenic
TGTATGAGAGGCACCAGCAA+TGG 0.552809 4:-38828478 MsG0480020317.01.T01:five_prime_UTR
GATGCTGCTGAAGGAATTTG+TGG 0.557209 4:-38827516 MsG0480020317.01.T01:CDS
TCTGCTGTTGCAGCAACCGA+AGG 0.563495 4:-38828035 MsG0480020317.01.T01:CDS
TGTCCCTGCTAACAATGAGC+AGG 0.563585 4:-38827775 MsG0480020317.01.T01:CDS
TAAGTGATTATTGGCAGCCA+TGG 0.564524 4:+38828583 None:intergenic
GAAGCCAATTACCCTTATCA+AGG 0.574336 4:-38827864 MsG0480020317.01.T01:CDS
TAAGACCACCCTCACAACCT+TGG 0.579957 4:+38827934 None:intergenic
TGGTTGGTTAAGAACTCTTG+GGG 0.580774 4:-38827585 MsG0480020317.01.T01:CDS
TGCGGAACTGAGTTGGATCA+TGG 0.581901 4:-38827654 MsG0480020317.01.T01:CDS
GAGAGGCACCAGCAATGGAT+GGG 0.587800 4:-38828473 MsG0480020317.01.T01:five_prime_UTR
AGATGTTGTGAACTCGTCGT+TGG 0.591287 4:+38828304 None:intergenic
ATGCCTGCTCATTGTTAGCA+GGG 0.591772 4:+38827772 None:intergenic
GACATCTTCATACCCAGTAA+TGG 0.594339 4:+38827794 None:intergenic
GATCATGGTGTTACCGCTGT+GGG 0.595355 4:-38827639 MsG0480020317.01.T01:CDS
AGAGGAGTTGATGCTGCTGA+AGG 0.602244 4:-38827525 MsG0480020317.01.T01:CDS
GAAGAAAGGAGCAGTGACAC+CGG 0.603366 4:-38828183 MsG0480020317.01.T01:CDS
GGATCATGGTGTTACCGCTG+TGG 0.606355 4:-38827640 MsG0480020317.01.T01:CDS
GGTGTGGACCAAGGTTGTGA+GGG 0.612032 4:-38827942 MsG0480020317.01.T01:CDS
TCAATGCAGTTACCATTACT+GGG 0.616110 4:-38827806 MsG0480020317.01.T01:CDS
GGAACATGCAACGCAAACAA+AGG 0.632542 4:-38827834 MsG0480020317.01.T01:CDS
CTCGTTGATTGTGACACGAA+AGG 0.633361 4:-38827963 MsG0480020317.01.T01:CDS
TTGTGAACTCGTCGTTGGTG+AGG 0.634830 4:+38828309 None:intergenic
ATATGTGCTCTTCGATCACA+AGG 0.635262 4:-38828262 MsG0480020317.01.T01:CDS
AGGTGTGGACCAAGGTTGTG+AGG 0.640947 4:-38827943 MsG0480020317.01.T01:CDS
CATCTAGAAACAAATTCAAG+GGG 0.641878 4:-38828286 MsG0480020317.01.T01:five_prime_UTR
AAGATGACTCCATGTATGAG+AGG 0.643922 4:-38828490 MsG0480020317.01.T01:five_prime_UTR
AATTGTTTGTCACACCACCA+TGG 0.652820 4:-38828600 MsG0480020317.01.T01:five_prime_UTR
AATAACAAGCTATACACACT+AGG 0.659674 4:-38828350 MsG0480020317.01.T01:five_prime_UTR
GTGATTATTGGCAGCCATGG+TGG 0.666651 4:+38828586 None:intergenic
GGTTATGGTGTTAGTAACGA+TGG 0.670080 4:-38827618 MsG0480020317.01.T01:CDS
GGATACATTATGATGCAAAG+AGG 0.676444 4:-38827543 MsG0480020317.01.T01:CDS
GTGACACCGGTGAAGAACCA+AGG 0.685861 4:-38828170 MsG0480020317.01.T01:CDS
GTGAAGAACCAAGGACAATG+TGG 0.702779 4:-38828161 MsG0480020317.01.T01:intron
TTTCTTCCTCCAATCCACCG+AGG 0.714688 4:+38828199 None:intergenic
GACACGAAAGGTGTGGACCA+AGG 0.716503 4:-38827951 MsG0480020317.01.T01:CDS
TGATTGTGACACGAAAGGTG+TGG 0.726786 4:-38827958 MsG0480020317.01.T01:CDS
AAGTGCAATTCCATCCTCGG+TGG 0.738684 4:-38828213 MsG0480020317.01.T01:CDS
ACTAACACCATAACCCACAG+CGG 0.792350 4:+38827626 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATGATATAAGTGATTAT+TGG + Chr4:38827405-38827424 None:intergenic 15.0%
!!! AATTTTAATTTTCCTTTTGT+AGG - Chr4:38827902-38827921 MsG0480020317.01.T01:CDS 15.0%
!! AAATTCATCATTCAAAATCA+TGG - Chr4:38828079-38828098 MsG0480020317.01.T01:intron 20.0%
!! TAATTCAAACTAAATTGTGA+TGG - Chr4:38828552-38828571 MsG0480020317.01.T01:five_prime_UTR 20.0%
!! TAGTTTGAATTACAAAGTTT+AGG + Chr4:38828544-38828563 None:intergenic 20.0%
! AACATCTAGAAACAAATTCA+AGG - Chr4:38827688-38827707 MsG0480020317.01.T01:CDS 25.0%
! ACATCTAGAAACAAATTCAA+GGG - Chr4:38827689-38827708 MsG0480020317.01.T01:CDS 25.0%
! AGTCAATTTATGAATTCCTT+CGG + Chr4:38827960-38827979 None:intergenic 25.0%
! TTATAAGGATTCTCAAGAAA+GGG - Chr4:38827541-38827560 MsG0480020317.01.T01:CDS 25.0%
! TTTATAAGGATTCTCAAGAA+AGG - Chr4:38827540-38827559 MsG0480020317.01.T01:CDS 25.0%
!! ACTTTTGTAGAATTGAAAGT+CGG + Chr4:38828284-38828303 None:intergenic 25.0%
!!! AATTTTGACATTTAAGCAGT+AGG + Chr4:38828508-38828527 None:intergenic 25.0%
!!! ATTTGCATTGTTTCTATGTT+TGG - Chr4:38827427-38827446 MsG0480020317.01.T01:three_prime_UTR 25.0%
!!! ATTTTGACATTTAAGCAGTA+GGG + Chr4:38828507-38828526 None:intergenic 25.0%
!!! TTTGCATTGTTTCTATGTTT+GGG - Chr4:38827428-38827447 MsG0480020317.01.T01:three_prime_UTR 25.0%
AATAACAAGCTATACACACT+AGG - Chr4:38827626-38827645 MsG0480020317.01.T01:CDS 30.0%
ACTAGGCATTAATCAATTTG+CGG - Chr4:38827643-38827662 MsG0480020317.01.T01:CDS 30.0%
CATCTAGAAACAAATTCAAG+GGG - Chr4:38827690-38827709 MsG0480020317.01.T01:CDS 30.0%
GACTTTCAATTCTACAAAAG+TGG - Chr4:38828283-38828302 MsG0480020317.01.T01:five_prime_UTR 30.0%
GGAATTCATAAATTGACTAC+CGG - Chr4:38827962-38827981 MsG0480020317.01.T01:CDS 30.0%
TAAATTGAAGCCTTCAACAA+AGG - Chr4:38827599-38827618 MsG0480020317.01.T01:CDS 30.0%
TCACTATGGCAAAGTTTATA+AGG - Chr4:38827526-38827545 MsG0480020317.01.T01:CDS 30.0%
TGATAAAGAGATCAACTTTC+CGG + Chr4:38827984-38828003 None:intergenic 30.0%
!! TGTGACAAACAATTGTTTTG+AGG + Chr4:38827370-38827389 None:intergenic 30.0%
AACACCTTGATAAGGGTAAT+TGG + Chr4:38828119-38828138 None:intergenic 35.0%
AGTAATGGTAACTGCATTGA+TGG + Chr4:38828170-38828189 None:intergenic 35.0%
ATCAATGCAGTTACCATTAC+TGG - Chr4:38828169-38828188 MsG0480020317.01.T01:CDS 35.0%
GGATACATTATGATGCAAAG+AGG - Chr4:38828433-38828452 MsG0480020317.01.T01:five_prime_UTR 35.0%
GTAACGATGGAACTAAGTAT+TGG - Chr4:38828371-38828390 MsG0480020317.01.T01:five_prime_UTR 35.0%
GTTATTGTCACCTTTGTTGA+AGG + Chr4:38827612-38827631 None:intergenic 35.0%
TCAATGCAGTTACCATTACT+GGG - Chr4:38828170-38828189 MsG0480020317.01.T01:CDS 35.0%
! ATTGGTTGGTTAAGAACTCT+TGG - Chr4:38828389-38828408 MsG0480020317.01.T01:five_prime_UTR 35.0%
! TACAAAAGTGGTGTTTTCAC+TGG - Chr4:38828295-38828314 MsG0480020317.01.T01:five_prime_UTR 35.0%
! TCCTTTTGTAGGATGTTGTT+GGG - Chr4:38827913-38827932 MsG0480020317.01.T01:CDS 35.0%
! TTCCTTTTGTAGGATGTTGT+TGG - Chr4:38827912-38827931 MsG0480020317.01.T01:CDS 35.0%
! TTGATAAGGGTAATTGGCTT+CGG + Chr4:38828113-38828132 None:intergenic 35.0%
! TTGGTTGGTTAAGAACTCTT+GGG - Chr4:38828390-38828409 MsG0480020317.01.T01:five_prime_UTR 35.0%
AAGATGACTCCATGTATGAG+AGG - Chr4:38827486-38827505 MsG0480020317.01.T01:CDS 40.0%
AATTGTTTGTCACACCACCA+TGG - Chr4:38827376-38827395 MsG0480020317.01.T01:three_prime_UTR 40.0%
ATATGTGCTCTTCGATCACA+AGG - Chr4:38827714-38827733 MsG0480020317.01.T01:CDS 40.0%
GAAGCCAATTACCCTTATCA+AGG - Chr4:38828112-38828131 MsG0480020317.01.T01:intron 40.0%
GACATCTTCATACCCAGTAA+TGG + Chr4:38828185-38828204 None:intergenic 40.0%
GCATCAATGGCTACAGAAAT+CGG + Chr4:38828253-38828272 None:intergenic 40.0%
TAAGTGATTATTGGCAGCCA+TGG + Chr4:38827396-38827415 None:intergenic 40.0%
TCTCATACATGGAGTCATCT+TGG + Chr4:38827487-38827506 None:intergenic 40.0%
TTCCGTCAACACCTTGATAA+GGG + Chr4:38828126-38828145 None:intergenic 40.0%
! GGTTATGGTGTTAGTAACGA+TGG - Chr4:38828358-38828377 MsG0480020317.01.T01:five_prime_UTR 40.0%
! TGGTTGGTTAAGAACTCTTG+GGG - Chr4:38828391-38828410 MsG0480020317.01.T01:five_prime_UTR 40.0%
!! CGATGGAACTAAGTATTGGT+TGG - Chr4:38828375-38828394 MsG0480020317.01.T01:five_prime_UTR 40.0%
!! TACCCTTATCAAGGTGTTGA+CGG - Chr4:38828121-38828140 MsG0480020317.01.T01:intron 40.0%
AACACGTACCACATTGTCCT+TGG + Chr4:38827826-38827845 None:intergenic 45.0%
AATGCCTGCTCATTGTTAGC+AGG + Chr4:38828208-38828227 None:intergenic 45.0%
ACTAACACCATAACCCACAG+CGG + Chr4:38828353-38828372 None:intergenic 45.0%
AGATGTTGTGAACTCGTCGT+TGG + Chr4:38827675-38827694 None:intergenic 45.0%
ATGCCTGCTCATTGTTAGCA+GGG + Chr4:38828207-38828226 None:intergenic 45.0%
GATGCTGCTGAAGGAATTTG+TGG - Chr4:38828460-38828479 MsG0480020317.01.T01:five_prime_UTR 45.0%
GCCCAACAACATCCTACAAA+AGG + Chr4:38827917-38827936 None:intergenic 45.0%
GGAACATGCAACGCAAACAA+AGG - Chr4:38828142-38828161 MsG0480020317.01.T01:intron 45.0%
GGCTACAGAAATCGGTTGAT+TGG + Chr4:38828245-38828264 None:intergenic 45.0%
GTATCCTTCTTCACCCCAAT+CGG + Chr4:38828419-38828438 None:intergenic 45.0%
GTGAAGAACCAAGGACAATG+TGG - Chr4:38827815-38827834 MsG0480020317.01.T01:CDS 45.0%
GTTCCGTCAACACCTTGATA+AGG + Chr4:38828127-38828146 None:intergenic 45.0%
TCTGTAGCCATTGATGCTAG+TGG - Chr4:38828256-38828275 MsG0480020317.01.T01:CDS 45.0%
TGATTGTGACACGAAAGGTG+TGG - Chr4:38828018-38828037 MsG0480020317.01.T01:CDS 45.0%
TGCAAGTGCAATTCCATCCT+CGG - Chr4:38827760-38827779 MsG0480020317.01.T01:CDS 45.0%
! AGAAATCGGTTGATTGGCCA+CGG + Chr4:38828239-38828258 None:intergenic 45.0%
! CATTGTCCTTGGTTCTTCAC+CGG + Chr4:38827815-38827834 None:intergenic 45.0%
! CTCGTTGATTGTGACACGAA+AGG - Chr4:38828013-38828032 MsG0480020317.01.T01:CDS 45.0%
! GGTGTTTTCACTGGATCATG+CGG - Chr4:38828304-38828323 MsG0480020317.01.T01:five_prime_UTR 45.0%
AACTCTTGGGGAACCGATTG+GGG - Chr4:38828403-38828422 MsG0480020317.01.T01:five_prime_UTR 50.0%
AAGTGCAATTCCATCCTCGG+TGG - Chr4:38827763-38827782 MsG0480020317.01.T01:CDS 50.0%
AGAACTCTTGGGGAACCGAT+TGG - Chr4:38828401-38828420 MsG0480020317.01.T01:five_prime_UTR 50.0%
CAATTCCATCCTCGGTGGAT+TGG - Chr4:38827768-38827787 MsG0480020317.01.T01:CDS 50.0%
CAGCAATGGATGGGTCACTA+TGG - Chr4:38827512-38827531 MsG0480020317.01.T01:CDS 50.0%
CATAGTGACCCATCCATTGC+TGG + Chr4:38827514-38827533 None:intergenic 50.0%
GAACTCTTGGGGAACCGATT+GGG - Chr4:38828402-38828421 MsG0480020317.01.T01:five_prime_UTR 50.0%
GAAGAAAGGAGCAGTGACAC+CGG - Chr4:38827793-38827812 MsG0480020317.01.T01:CDS 50.0%
GTCGGAACCACTAGCATCAA+TGG + Chr4:38828266-38828285 None:intergenic 50.0%
GTGATTATTGGCAGCCATGG+TGG + Chr4:38827393-38827412 None:intergenic 50.0%
TAAGACCACCCTCACAACCT+TGG + Chr4:38828045-38828064 None:intergenic 50.0%
TGGATCATGCGGAACTGAGT+TGG - Chr4:38828315-38828334 MsG0480020317.01.T01:five_prime_UTR 50.0%
TGTCCCTGCTAACAATGAGC+AGG - Chr4:38828201-38828220 MsG0480020317.01.T01:CDS 50.0%
TTGCTGGTGCCTCTCATACA+TGG + Chr4:38827498-38827517 None:intergenic 50.0%
TTGTGAACTCGTCGTTGGTG+AGG + Chr4:38827670-38827689 None:intergenic 50.0%
TTTCTTCCTCCAATCCACCG+AGG + Chr4:38827780-38827799 None:intergenic 50.0%
! AGGTTGTGAGGGTGGTCTTA+TGG - Chr4:38828045-38828064 MsG0480020317.01.T01:CDS 50.0%
! TGCGGAACTGAGTTGGATCA+TGG - Chr4:38828322-38828341 MsG0480020317.01.T01:five_prime_UTR 50.0%
!! AGAGGAGTTGATGCTGCTGA+AGG - Chr4:38828451-38828470 MsG0480020317.01.T01:five_prime_UTR 50.0%
!! GATCATGGTGTTACCGCTGT+GGG - Chr4:38828337-38828356 MsG0480020317.01.T01:five_prime_UTR 50.0%
!! TCGGTGGATTGGAGGAAGAA+AGG - Chr4:38827779-38827798 MsG0480020317.01.T01:CDS 50.0%
!! TGTATGAGAGGCACCAGCAA+TGG - Chr4:38827498-38827517 MsG0480020317.01.T01:CDS 50.0%
AGGTGTGGACCAAGGTTGTG+AGG - Chr4:38828033-38828052 MsG0480020317.01.T01:CDS 55.0%
GGAACCGATTGGGGTGAAGA+AGG - Chr4:38828412-38828431 MsG0480020317.01.T01:five_prime_UTR 55.0%
GGTGTGGACCAAGGTTGTGA+GGG - Chr4:38828034-38828053 MsG0480020317.01.T01:CDS 55.0%
GTGACACCGGTGAAGAACCA+AGG - Chr4:38827806-38827825 MsG0480020317.01.T01:CDS 55.0%
TCTGCTGTTGCAGCAACCGA+AGG - Chr4:38827941-38827960 MsG0480020317.01.T01:CDS 55.0%
TTCCTCCAATCCACCGAGGA+TGG + Chr4:38827776-38827795 None:intergenic 55.0%
! GACACGAAAGGTGTGGACCA+AGG - Chr4:38828025-38828044 MsG0480020317.01.T01:CDS 55.0%
! GCAGGCATTGCAAAAAGCCG+TGG - Chr4:38828219-38828238 MsG0480020317.01.T01:CDS 55.0%
! GGTGTTACCGCTGTGGGTTA+TGG - Chr4:38828343-38828362 MsG0480020317.01.T01:five_prime_UTR 55.0%
!! GAGAGGCACCAGCAATGGAT+GGG - Chr4:38827503-38827522 MsG0480020317.01.T01:CDS 55.0%
!! GGATCATGGTGTTACCGCTG+TGG - Chr4:38828336-38828355 MsG0480020317.01.T01:five_prime_UTR 55.0%
!! TGAGAGGCACCAGCAATGGA+TGG - Chr4:38827502-38827521 MsG0480020317.01.T01:CDS 55.0%
!! TTCCATCCTCGGTGGATTGG+AGG - Chr4:38827771-38827790 MsG0480020317.01.T01:CDS 55.0%
GTGGACCAAGGTTGTGAGGG+TGG - Chr4:38828037-38828056 MsG0480020317.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 38827368 38828630 38827368 ID=MsG0480020317.01;Name=MsG0480020317.01
Chr4 mRNA 38827368 38828630 38827368 ID=MsG0480020317.01.T01;Parent=MsG0480020317.01;Name=MsG0480020317.01.T01;_AED=0.47;_eAED=0.47;_QI=348|1|1|1|1|1|2|114|237
Chr4 exon 38827368 38828074 38827368 ID=MsG0480020317.01.T01:exon:3904;Parent=MsG0480020317.01.T01
Chr4 exon 38828162 38828630 38828162 ID=MsG0480020317.01.T01:exon:3903;Parent=MsG0480020317.01.T01
Chr4 five_prime_UTR 38828283 38828630 38828283 ID=MsG0480020317.01.T01:five_prime_utr;Parent=MsG0480020317.01.T01
Chr4 CDS 38828162 38828282 38828162 ID=MsG0480020317.01.T01:cds;Parent=MsG0480020317.01.T01
Chr4 CDS 38827482 38828074 38827482 ID=MsG0480020317.01.T01:cds;Parent=MsG0480020317.01.T01
Chr4 three_prime_UTR 38827368 38827481 38827368 ID=MsG0480020317.01.T01:three_prime_utr;Parent=MsG0480020317.01.T01
Gene Sequence

>MsG0480020317.01.T01

ATGTGCTCTTCGATCACAAGGACAAGTACTTTTAAGTATGAAAATGCAAGTGCAATTCCATCCTCGGTGGATTGGAGGAAGAAAGGAGCAGTGACACCGGTGAAGAACCAAGGACAATGTGGATGTTGTTGGGCGTTTTCTGCTGTTGCAGCAACCGAAGGAATTCATAAATTGACTACCGGAAAGTTGATCTCTTTATCAGAACAAGAACTCGTTGATTGTGACACGAAAGGTGTGGACCAAGGTTGTGAGGGTGGTCTTATGGACGATGCTTTCAAATTCATCATTCAAAATCATGGACTCGCTACCGAAGCCAATTACCCTTATCAAGGTGTTGACGGAACATGCAACGCAAACAAAGGATCCATCAATGCAGTTACCATTACTGGGTATGAAGATGTCCCTGCTAACAATGAGCAGGCATTGCAAAAAGCCGTGGCCAATCAACCGATTTCTGTAGCCATTGATGCTAGTGGTTCCGACTTTCAATTCTACAAAAGTGGTGTTTTCACTGGATCATGCGGAACTGAGTTGGATCATGGTGTTACCGCTGTGGGTTATGGTGTTAGTAACGATGGAACTAAGTATTGGTTGGTTAAGAACTCTTGGGGAACCGATTGGGGTGAAGAAGGATACATTATGATGCAAAGAGGAGTTGATGCTGCTGAAGGAATTTGTGGCATTGCAATGCAAGCATCATACCCTACTGCTTAA
Protein sequence

>MsG0480020317.01.T01

MCSSITRTSTFKYENASAIPSSVDWRKKGAVTPVKNQGQCGCCWAFSAVAATEGIHKLTTGKLISLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGLATEANYPYQGVDGTCNANKGSINAVTITGYEDVPANNEQALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGVSNDGTKYWLVKNSWGTDWGEEGYIMMQRGVDAAEGICGIAMQASYPTA*