AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020517.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020517.01.T01 MTR_2g086680 68.023 172 53 2 1 170 1 172 1.02e-83 244
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020517.01.T01 AT5G22430 47.134 157 81 2 15 170 17 172 2.18e-45 147
MsG0480020517.01.T01 AT2G27385 45.181 166 82 3 5 170 6 162 2.78e-41 136
MsG0480020517.01.T01 AT2G27385 45.181 166 82 3 5 170 17 173 4.63e-41 136
MsG0480020517.01.T01 AT2G27385 45.181 166 82 3 5 170 17 173 4.63e-41 136

Find 30 sgRNAs with CRISPR-Local

Find 96 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCCTTACAATTTGTGTTT+TGG 0.164797 4:+42782538 None:intergenic
CAAGTTTCACAAGTTATTAA+AGG 0.220047 4:-42782618 MsG0480020517.01.T01:CDS
TGAACACACATGATTAATTA+AGG 0.275899 4:+42782411 None:intergenic
ATCAGCTTGTGAAATTGTTC+AGG 0.288968 4:-42783638 MsG0480020517.01.T01:CDS
AACTGTTTGGCAAAACTTCT+TGG 0.296592 4:-42782675 MsG0480020517.01.T01:CDS
CAAAGGAAGATCAATAGTTT+TGG 0.317110 4:+42782497 None:intergenic
TCTTCCTTTGCCTCCAGAAT+GGG 0.330802 4:-42782484 MsG0480020517.01.T01:CDS
CTAGGTGCCAAACCCCATTC+TGG 0.344968 4:+42782471 None:intergenic
TGTAAGGCTGCAAAACCTAT+TGG 0.353116 4:-42782525 MsG0480020517.01.T01:CDS
CTTTAATAACTTGTGAAACT+TGG 0.355048 4:+42782619 None:intergenic
AATTAAGGTATTCCAATGAT+AGG 0.380107 4:+42782426 None:intergenic
ACACATAGCTACGATTTATC+TGG 0.387065 4:-42783592 MsG0480020517.01.T01:intron
TTTGCCTCCAGAATGGGGTT+TGG 0.402516 4:-42782478 MsG0480020517.01.T01:CDS
ATCTTCCTTTGCCTCCAGAA+TGG 0.404991 4:-42782485 MsG0480020517.01.T01:CDS
CTTCTGTAGTTGTCATAGCT+AGG 0.461561 4:+42782751 None:intergenic
TGAGGGAAGGTTAACCTTGA+AGG 0.474424 4:+42782719 None:intergenic
GAACAATTTCACAAGCTGAT+AGG 0.484005 4:+42783641 None:intergenic
TGTTTGGCAAAACTTCTTGG+TGG 0.491974 4:-42782672 MsG0480020517.01.T01:CDS
CTTCCTTTGCCTCCAGAATG+GGG 0.503336 4:-42782483 MsG0480020517.01.T01:CDS
ATTCCAATGATAGGAACAAA+AGG 0.504181 4:+42782435 None:intergenic
ATTAATCATGTGTGTTCAAG+TGG 0.508053 4:-42782406 MsG0480020517.01.T01:three_prime_UTR
AGAAGAAGATGGCTCCTTCA+AGG 0.515472 4:-42782733 MsG0480020517.01.T01:CDS
TCAGCTTGTGAAATTGTTCA+GGG 0.520217 4:-42783637 MsG0480020517.01.T01:CDS
CATGATGTCAAGAAACTAAG+AGG 0.549180 4:+42782564 None:intergenic
AAACCCCATTCTGGAGGCAA+AGG 0.556721 4:+42782480 None:intergenic
AAAGGTACATAATAGCTACT+AGG 0.564274 4:+42782453 None:intergenic
ATGAATCTTATATGTAGCTG+TGG 0.599675 4:-42782304 MsG0480020517.01.T01:three_prime_UTR
ATGACAACTACAGAAGAAGA+TGG 0.604128 4:-42782744 MsG0480020517.01.T01:CDS
AAGGTCACAGTGAAATGTGA+AGG 0.615631 4:-42782783 MsG0480020517.01.T01:CDS
GGTGCCAAACCCCATTCTGG+AGG 0.664114 4:+42782474 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATAGTTTATTTATTAATA+GGG - Chr4:42782529-42782548 MsG0480020517.01.T01:CDS 10.0%
!! TCAATAGTTTATTTATTAAT+AGG - Chr4:42782528-42782547 MsG0480020517.01.T01:CDS 10.0%
!!! TTTTTTTTTTTGTTTCTTTT+CGG + Chr4:42782890-42782909 None:intergenic 10.0%
!! ACTTGAGTAATAAAAATTAT+AGG - Chr4:42783556-42783575 MsG0480020517.01.T01:intron 15.0%
!! CTTGAGTAATAAAAATTATA+GGG - Chr4:42783557-42783576 MsG0480020517.01.T01:intron 15.0%
!! AATAGTAGACATATGTATTA+TGG - Chr4:42782445-42782464 MsG0480020517.01.T01:CDS 20.0%
!! ATCATGTATGTTTCTTTAAA+AGG + Chr4:42782650-42782669 None:intergenic 20.0%
!! GAACATTTAATTAAACTCAA+AGG + Chr4:42782393-42782412 None:intergenic 20.0%
!! TACAAGATGCATATAATATA+TGG + Chr4:42783089-42783108 None:intergenic 20.0%
!! TTTGTTAAGAAATACACATT+TGG - Chr4:42782703-42782722 MsG0480020517.01.T01:CDS 20.0%
!!! AATTTTTTTAGAAAGAGTGT+GGG - Chr4:42782944-42782963 MsG0480020517.01.T01:intron 20.0%
!!! TAATTTTTTTAGAAAGAGTG+TGG - Chr4:42782943-42782962 MsG0480020517.01.T01:intron 20.0%
!!! TCAATGATTTTCAGATATTA+AGG - Chr4:42783128-42783147 MsG0480020517.01.T01:intron 20.0%
!!! TTTTTAATGTCCCTAATTAT+GGG - Chr4:42782614-42782633 MsG0480020517.01.T01:CDS 20.0%
!!! TTTTTTAATGTCCCTAATTA+TGG - Chr4:42782613-42782632 MsG0480020517.01.T01:CDS 20.0%
! AAGGCCTTTACAAAAAATTA+CGG + Chr4:42782796-42782815 None:intergenic 25.0%
! CAAGTTTCACAAGTTATTAA+AGG - Chr4:42783312-42783331 MsG0480020517.01.T01:intron 25.0%
! TAAAAAAATTACATGTGTCC+CGG + Chr4:42782934-42782953 None:intergenic 25.0%
! TAAAATTGCTCCCATAATTA+GGG + Chr4:42782627-42782646 None:intergenic 25.0%
! TGAACACACATGATTAATTA+AGG + Chr4:42783522-42783541 None:intergenic 25.0%
!! AATTAAGGTATTCCAATGAT+AGG + Chr4:42783507-42783526 None:intergenic 25.0%
!! CTTTAATAACTTGTGAAACT+TGG + Chr4:42783314-42783333 None:intergenic 25.0%
!!! AAAAATACTAGAGCTTTGTT+TGG + Chr4:42782598-42782617 None:intergenic 25.0%
!!! ACAATAGTTTTTGTGTTGTT+TGG + Chr4:42782683-42782702 None:intergenic 25.0%
AAAAACTTCCATGAACTGTT+TGG - Chr4:42783242-42783261 MsG0480020517.01.T01:intron 30.0%
AAAAGTCTCAAAGTATACTC+CGG - Chr4:42782912-42782931 MsG0480020517.01.T01:intron 30.0%
AGGCCTTTACAAAAAATTAC+GGG + Chr4:42782795-42782814 None:intergenic 30.0%
ATGAATCTTATATGTAGCTG+TGG - Chr4:42783626-42783645 MsG0480020517.01.T01:CDS 30.0%
ATTAATCATGTGTGTTCAAG+TGG - Chr4:42783524-42783543 MsG0480020517.01.T01:intron 30.0%
ATTCCAATGATAGGAACAAA+AGG + Chr4:42783498-42783517 None:intergenic 30.0%
GTAAAATTGCTCCCATAATT+AGG + Chr4:42782628-42782647 None:intergenic 30.0%
TTGTTTGGAGATTAAAGCTA+GGG + Chr4:42782583-42782602 None:intergenic 30.0%
TTTATACCAGATGTCTCTTT+CGG + Chr4:42782559-42782578 None:intergenic 30.0%
TTTGTTTGGAGATTAAAGCT+AGG + Chr4:42782584-42782603 None:intergenic 30.0%
! AAAGGTACATAATAGCTACT+AGG + Chr4:42783480-42783499 None:intergenic 30.0%
! AGCCTTACAATTTGTGTTTT+GGG + Chr4:42783394-42783413 None:intergenic 30.0%
! CAAAGGAAGATCAATAGTTT+TGG + Chr4:42783436-42783455 None:intergenic 30.0%
!! TTTTTGGCATAAAGCTGAAT+TGG + Chr4:42783285-42783304 None:intergenic 30.0%
!!! ATAGTTTTGGAAGAACCAAT+AGG + Chr4:42783423-42783442 None:intergenic 30.0%
!!! TGAAACTTGGTTTTGCTTTT+TGG + Chr4:42783301-42783320 None:intergenic 30.0%
AAAGTCTCAAAGTATACTCC+GGG - Chr4:42782913-42782932 MsG0480020517.01.T01:intron 35.0%
AACTGTTTGGCAAAACTTCT+TGG - Chr4:42783255-42783274 MsG0480020517.01.T01:intron 35.0%
ACACATAGCTACGATTTATC+TGG - Chr4:42782338-42782357 MsG0480020517.01.T01:three_prime_UTR 35.0%
ATCAGCTTGTGAAATTGTTC+AGG - Chr4:42782292-42782311 MsG0480020517.01.T01:three_prime_UTR 35.0%
ATGACAACTACAGAAGAAGA+TGG - Chr4:42783186-42783205 MsG0480020517.01.T01:intron 35.0%
CATCGACCATTTGATACATA+AGG + Chr4:42783016-42783035 None:intergenic 35.0%
CATGATGTCAAGAAACTAAG+AGG + Chr4:42783369-42783388 None:intergenic 35.0%
CCTTTACAAAAAATTACGGG+AGG + Chr4:42782792-42782811 None:intergenic 35.0%
CTTAAGGTTCCAAGAATGTT+GGG - Chr4:42782986-42783005 MsG0480020517.01.T01:intron 35.0%
GAACAATTTCACAAGCTGAT+AGG + Chr4:42782292-42782311 None:intergenic 35.0%
GTATACTTCTAGACACATGT+TGG + Chr4:42782865-42782884 None:intergenic 35.0%
TACCTTGGAGATTTCACTTA+TGG - Chr4:42782824-42782843 MsG0480020517.01.T01:intron 35.0%
TCAGCTTGTGAAATTGTTCA+GGG - Chr4:42782293-42782312 MsG0480020517.01.T01:three_prime_UTR 35.0%
TTGAAGAACAATGAGAGTCA+TGG + Chr4:42783112-42783131 None:intergenic 35.0%
! AAGTTTTGCCAAACAGTTCA+TGG + Chr4:42783253-42783272 None:intergenic 35.0%
! CAGCCTTACAATTTGTGTTT+TGG + Chr4:42783395-42783414 None:intergenic 35.0%
! GAAGTTTTTGTGTTGTCTGA+GGG + Chr4:42783231-42783250 None:intergenic 35.0%
! GCCTTACAATTTGTGTTTTG+GGG + Chr4:42783393-42783412 None:intergenic 35.0%
! GTACCTTTTGTTCCTATCAT+TGG - Chr4:42783492-42783511 MsG0480020517.01.T01:intron 35.0%
! TCTCCAAGGTATTATCAACA+AGG + Chr4:42782815-42782834 None:intergenic 35.0%
!! CCTTACAATTTGTGTTTTGG+GGG + Chr4:42783392-42783411 None:intergenic 35.0%
!!! AAGCTGTGTTTTTTCCCATA+TGG - Chr4:42782735-42782754 MsG0480020517.01.T01:CDS 35.0%
!!! AGCTGTGTTTTTTCCCATAT+GGG - Chr4:42782736-42782755 MsG0480020517.01.T01:CDS 35.0%
!!! CCTCCCGTAATTTTTTGTAA+AGG - Chr4:42782789-42782808 MsG0480020517.01.T01:CDS 35.0%
AAGGTCACAGTGAAATGTGA+AGG - Chr4:42783147-42783166 MsG0480020517.01.T01:intron 40.0%
ACTTCTAGACACATGTTGGT+TGG + Chr4:42782861-42782880 None:intergenic 40.0%
CCAACATTCTTGGAACCTTA+AGG + Chr4:42782988-42783007 None:intergenic 40.0%
CCCCCAAAACACAAATTGTA+AGG - Chr4:42783389-42783408 MsG0480020517.01.T01:intron 40.0%
CCTTAAGGTTCCAAGAATGT+TGG - Chr4:42782985-42783004 MsG0480020517.01.T01:intron 40.0%
CTCCATAAGTGAAATCTCCA+AGG + Chr4:42782829-42782848 None:intergenic 40.0%
CTTCTGTAGTTGTCATAGCT+AGG + Chr4:42783182-42783201 None:intergenic 40.0%
CTTTGTCACTCAGAAGAGTT+CGG + Chr4:42782767-42782786 None:intergenic 40.0%
GACCATTTGATACATAAGGC+CGG + Chr4:42783012-42783031 None:intergenic 40.0%
TGTAAGGCTGCAAAACCTAT+TGG - Chr4:42783405-42783424 MsG0480020517.01.T01:intron 40.0%
TGTTTGGCAAAACTTCTTGG+TGG - Chr4:42783258-42783277 MsG0480020517.01.T01:intron 40.0%
! CTTCTAGACACATGTTGGTT+GGG + Chr4:42782860-42782879 None:intergenic 40.0%
! GGAAGTTTTTGTGTTGTCTG+AGG + Chr4:42783232-42783251 None:intergenic 40.0%
! TTTTTGTGTTGTCTGAGGGA+AGG + Chr4:42783227-42783246 None:intergenic 40.0%
!! AGGCCTTGTTGATAATACCT+TGG - Chr4:42782809-42782828 MsG0480020517.01.T01:intron 40.0%
AAAGAAGCTCGCTTGCCTTA+AGG - Chr4:42782970-42782989 MsG0480020517.01.T01:intron 45.0%
AGAAGAAGATGGCTCCTTCA+AGG - Chr4:42783197-42783216 MsG0480020517.01.T01:intron 45.0%
AGAGTTCGGATGTACCCATA+TGG + Chr4:42782753-42782772 None:intergenic 45.0%
ATCTTCCTTTGCCTCCAGAA+TGG - Chr4:42783445-42783464 MsG0480020517.01.T01:intron 45.0%
GAGTTCGGATGTACCCATAT+GGG + Chr4:42782752-42782771 None:intergenic 45.0%
TACCAGATGTCTCTTTCGGT+CGG + Chr4:42782555-42782574 None:intergenic 45.0%
TCTTCCTTTGCCTCCAGAAT+GGG - Chr4:42783446-42783465 MsG0480020517.01.T01:intron 45.0%
TGAGGGAAGGTTAACCTTGA+AGG + Chr4:42783214-42783233 None:intergenic 45.0%
AGGTTCCAAGAATGTTGGGC+CGG - Chr4:42782990-42783009 MsG0480020517.01.T01:intron 50.0%
CTTCCTTTGCCTCCAGAATG+GGG - Chr4:42783447-42783466 MsG0480020517.01.T01:intron 50.0%
TTTGCCTCCAGAATGGGGTT+TGG - Chr4:42783452-42783471 MsG0480020517.01.T01:intron 50.0%
! AAACCCCATTCTGGAGGCAA+AGG + Chr4:42783453-42783472 None:intergenic 50.0%
! GGCCGGCCTTATGTATCAAA+TGG - Chr4:42783007-42783026 MsG0480020517.01.T01:intron 50.0%
CTAGGTGCCAAACCCCATTC+TGG + Chr4:42783462-42783481 None:intergenic 55.0%
TAAGGCCGGCCCAACATTCT+TGG + Chr4:42782998-42783017 None:intergenic 55.0%
GGCCGACCGAAAGAGACATC+TGG - Chr4:42782550-42782569 MsG0480020517.01.T01:CDS 60.0%
GGTGCCAAACCCCATTCTGG+AGG + Chr4:42783459-42783478 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 42782230 42783722 42782230 ID=MsG0480020517.01;Name=MsG0480020517.01
Chr4 mRNA 42782230 42783722 42782230 ID=MsG0480020517.01.T01;Parent=MsG0480020517.01;Name=MsG0480020517.01.T01;_AED=0.37;_eAED=0.37;_QI=0|1|0.5|1|1|1|2|198|170
Chr4 exon 42782230 42782810 42782230 ID=MsG0480020517.01.T01:exon:4622;Parent=MsG0480020517.01.T01
Chr4 exon 42783593 42783722 42783593 ID=MsG0480020517.01.T01:exon:4621;Parent=MsG0480020517.01.T01
Chr4 CDS 42783593 42783722 42783593 ID=MsG0480020517.01.T01:cds;Parent=MsG0480020517.01.T01
Chr4 CDS 42782428 42782810 42782428 ID=MsG0480020517.01.T01:cds;Parent=MsG0480020517.01.T01
Chr4 three_prime_UTR 42782230 42782427 42782230 ID=MsG0480020517.01.T01:three_prime_utr;Parent=MsG0480020517.01.T01
Gene Sequence

>MsG0480020517.01.T01

ATGGCATATGTTCATGTTATCACAGCTCTTCTCTTTGCATTAGTTCTAGTTAGAATTGACCTATCAGCTTGTGAAATTGTTCAGGGTAAAGTCTCATGTGTTGACTGCACACATAGCTACGATTTATCTGATATTAAGGTCACAGTGAAATGTGAAGGTGTTAAAAACCTAGCTATGACAACTACAGAAGAAGATGGCTCCTTCAAGGTTAACCTTCCCTCAGACAACACAAAAACTTCCATGAACTGTTTGGCAAAACTTCTTGGTGGACCAATTCAGCTTTATGCCAAAAAGCAAAACCAAGTTTCACAAGTTATTAAAGGAAAAGAAGAAAACATCTACACAATCTCAACTCCTCTTAGTTTCTTGACATCATGCCCCCAAAACACAAATTGTAAGGCTGCAAAACCTATTGGTTCTTCCAAAACTATTGATCTTCCTTTGCCTCCAGAATGGGGTTTGGCACCTAGTAGCTATTATGTACCTTTTGTTCCTATCATTGGAATACCTTAA

Protein sequence

>MsG0480020517.01.T01

MAYVHVITALLFALVLVRIDLSACEIVQGKVSCVDCTHSYDLSDIKVTVKCEGVKNLAMTTTEEDGSFKVNLPSDNTKTSMNCLAKLLGGPIQLYAKKQNQVSQVIKGKEENIYTISTPLSFLTSCPQNTNCKAAKPIGSSKTIDLPLPPEWGLAPSSYYVPFVPIIGIP*