AlfalfaGEDB | MsG0480021158.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021158.01

KEGG: -
GO: -
PFAM: PF06075

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021158.01.T01	XP_003606816.1	95.873	727	28	1	1	725	1	727	0	1422

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021158.01.T01	G7JEV5	95.873	727	28	1	1	725	1	727	0.0	1422

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048700.01	MsG0480021158.01	0.807206	5.661116e-50	3.134198e-47
MsG0180000001.01	MsG0480021158.01	0.802107	6.563578e-49	3.188121e-46
MsG0180000360.01	MsG0480021158.01	0.844771	6.149227e-59	1.003258e-55
MsG0180001117.01	MsG0480021158.01	0.814510	1.485254e-51	9.985945e-49
MsG0180001173.01	MsG0480021158.01	0.819275	1.265062e-52	9.690632e-50
MsG0180001565.01	MsG0480021158.01	0.800258	1.567874e-48	7.267228e-46
MsG0180003168.01	MsG0480021158.01	0.816578	5.145088e-52	3.659703e-49
MsG0180003820.01	MsG0480021158.01	0.813718	2.221052e-51	1.461562e-48
MsG0180004357.01	MsG0480021158.01	0.850819	1.326569e-60	2.631491e-57
MsG0180004989.01	MsG0480021158.01	0.808974	2.378508e-50	1.379274e-47
MsG0180005188.01	MsG0480021158.01	0.831250	1.857254e-55	2.004004e-52
MsG0180005921.01	MsG0480021158.01	0.818227	2.188614e-52	1.628663e-49
MsG0180006260.01	MsG0480021158.01	0.814023	1.902303e-51	1.262154e-48
MsG0480021156.01	MsG0480021158.01	0.847451	1.146705e-59	2.039120e-56
MsG0480021158.01	MsG0480022325.01	0.845510	3.881631e-59	6.483779e-56
MsG0480021158.01	MsG0480022952.01	0.808592	2.870966e-50	1.648126e-47
MsG0480021158.01	MsG0480023053.01	0.859788	3.246067e-63	8.676950e-60
MsG0480021158.01	MsG0480023541.01	0.830337	3.108510e-55	3.265599e-52
MsG0480021158.01	MsG0480023620.01	0.891159	5.120874e-74	4.438636e-70
MsG0480021158.01	MsG0480023874.01	0.800374	1.485427e-48	6.905427e-46
MsG0480021158.01	MsG0580025352.01	0.824380	8.327624e-54	7.363924e-51
MsG0480021158.01	MsG0580027208.01	0.815268	1.008419e-51	6.920219e-49
MsG0480021158.01	MsG0580027933.01	0.813750	2.185331e-51	1.439203e-48
MsG0480021158.01	MsG0580028060.01	0.845623	3.617909e-59	6.064947e-56
MsG0480021158.01	MsG0680033793.01	0.803181	3.940604e-49	1.967228e-46
MsG0480021158.01	MsG0780036752.01	0.822739	2.016210e-53	1.702190e-50
MsG0480021158.01	MsG0780036816.01	0.825683	4.100393e-54	3.763748e-51
MsG0480021158.01	MsG0780038603.01	0.818565	1.834673e-52	1.378100e-49
MsG0480021158.01	MsG0780039655.01	0.810470	1.134399e-50	6.843894e-48
MsG0480021158.01	MsG0780039712.01	0.804160	2.468790e-49	1.263849e-46
MsG0480021158.01	MsG0780040564.01	0.806728	7.143775e-50	3.906369e-47
MsG0480021158.01	MsG0780040586.01	0.803871	2.834362e-49	1.440349e-46
MsG0480021158.01	MsG0780040880.01	0.806905	6.554349e-50	3.600462e-47
MsG0480021158.01	MsG0880042791.01	0.813534	2.437652e-51	1.596131e-48
MsG0480021158.01	MsG0880042845.01	0.841046	6.023754e-58	8.743124e-55
MsG0480021158.01	MsG0880042929.01	0.803506	3.373865e-49	1.698495e-46
MsG0480021158.01	MsG0880044782.01	0.812233	4.700803e-51	2.972437e-48
MsG0480021158.01	MsG0880045838.01	0.811371	7.240763e-51	4.474053e-48
MsG0480021158.01	MsG0880046762.01	0.813486	2.498244e-51	1.633673e-48
MsG0480021158.01	MsG0880047226.01	0.823179	1.591829e-53	1.360644e-50
MsG0480021158.01	MsG0880047293.01	0.813449	2.545676e-51	1.663020e-48
MsG0380015548.01	MsG0480021158.01	0.813503	2.476830e-51	1.620392e-48
MsG0380016247.01	MsG0480021158.01	0.808971	2.382941e-50	1.381709e-47
MsG0380016703.01	MsG0480021158.01	0.837991	3.747683e-57	4.952296e-54
MsG0380016994.01	MsG0480021158.01	0.808925	2.437505e-50	1.411570e-47
MsG0380017159.01	MsG0480021158.01	0.845302	4.420324e-59	7.334328e-56
MsG0380017239.01	MsG0480021158.01	0.806208	9.198557e-50	4.963770e-47
MsG0380017240.01	MsG0480021158.01	0.851244	1.007181e-60	2.025881e-57
MsG0480018256.01	MsG0480021158.01	0.802421	5.654290e-49	2.768730e-46
MsG0280007790.01	MsG0480021158.01	0.804639	1.961033e-49	1.016369e-46
MsG0280008614.01	MsG0480021158.01	0.803546	3.311557e-49	1.668810e-46
MsG0280009156.01	MsG0480021158.01	0.807901	4.031201e-50	2.272404e-47
MsG0280009514.01	MsG0480021158.01	0.818923	1.521277e-52	1.154225e-49
MsG0280010001.01	MsG0480021158.01	0.838527	2.727366e-57	3.662944e-54

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021158.01.T01	MTR_4g068050	95.873	727	28	1	1	725	1	727	0.0	1422
MsG0480021158.01.T01	MTR_2g099095	62.681	761	209	17	1	718	1	729	0.0	816
MsG0480021158.01.T01	MTR_0050s0010	63.025	119	44	0	1	119	1	119	1.15e-47	177
MsG0480021158.01.T01	MTR_0050s0010	35.833	120	60	3	608	712	412	529	3.98e-12	69.7
MsG0480021158.01.T01	MTR_5g034370	57.025	121	51	1	1	120	1	121	2.12e-41	160
MsG0480021158.01.T01	MTR_4g112035	56.667	120	52	0	1	120	1	120	4.67e-40	157
MsG0480021158.01.T01	MTR_2g062300	31.680	363	196	13	369	711	254	584	1.08e-36	146
MsG0480021158.01.T01	MTR_2g062300	46.825	126	61	2	1	124	1	122	1.85e-26	115

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021158.01.T01	AT1G08760	51.747	744	298	17	1	706	1	721	0.0	676
MsG0480021158.01.T01	AT3G14170	61.345	119	46	0	1	119	1	119	2.81e-45	170
MsG0480021158.01.T01	AT3G14170	35.338	133	69	3	594	712	374	503	5.12e-13	72.8
MsG0480021158.01.T01	AT3G14170	34.831	89	58	0	384	472	250	338	1.68e-12	70.9
MsG0480021158.01.T01	AT3G14170	61.345	119	46	0	1	119	1	119	2.91e-45	169
MsG0480021158.01.T01	AT3G14170	35.338	133	69	3	594	712	375	504	5.46e-13	72.4
MsG0480021158.01.T01	AT3G14170	34.831	89	58	0	384	472	251	339	1.84e-12	70.9
MsG0480021158.01.T01	AT4G13370	58.400	125	52	0	1	125	1	125	3.01e-42	164
MsG0480021158.01.T01	AT4G13370	28.135	327	160	6	382	707	413	665	2.20e-23	105
MsG0480021158.01.T01	AT1G23790	57.377	122	51	1	1	121	1	122	3.43e-41	158
MsG0480021158.01.T01	AT1G70340	57.500	120	50	1	1	119	1	120	1.29e-39	153
MsG0480021158.01.T01	AT3G19610	48.333	120	61	1	1	119	29	148	6.86e-36	144
MsG0480021158.01.T01	AT3G19610	27.108	332	174	9	390	709	335	610	2.28e-22	102
MsG0480021158.01.T01	AT2G31920	44.697	132	64	4	1	124	1	131	1.83e-25	112
MsG0480021158.01.T01	AT2G31920	27.300	337	191	11	376	708	284	570	2.65e-21	99.0

Find 151 sgRNAs with CRISPR-Local

Find 241 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGGAGTTCTGATTATTTATT+TGG	0.095740	4:-54526468	MsG0480021158.01.T01:three_prime_UTR
CAAAATCAAAAGATTGTTCT+TGG	0.220021	4:-54527200	MsG0480021158.01.T01:CDS
TGAAACCTTGACAAAGTTTC+CGG	0.253676	4:+54528774	None:intergenic
GTTGTGATTCTTTCATTATC+TGG	0.256643	4:+54527371	None:intergenic
CCACTCCAAGAGTGGATTAA+AGG	0.275711	4:-54527014	MsG0480021158.01.T01:CDS
CTTACTGTGCTACGAAATTC+AGG	0.277895	4:+54529449	None:intergenic
CTTAGAAGATGCCCTTGATT+TGG	0.289921	4:+54529484	None:intergenic
ATAGTTTGCTACAATGTCTT+AGG	0.295406	4:-54528117	MsG0480021158.01.T01:intron
TAGCTTAAGATGCATTATTA+TGG	0.310907	4:-54526594	MsG0480021158.01.T01:three_prime_UTR
AAATCATCCGCGCACTATTC+AGG	0.311812	4:+54527432	None:intergenic
CGTGGTGTTAGGCCTGTTCC+TGG	0.317826	4:-54530055	MsG0480021158.01.T01:CDS
GCATACAATAACTTTATAAA+AGG	0.322933	4:+54526404	None:intergenic
GGAGTTCTGATTATTTATTT+GGG	0.327095	4:-54526467	MsG0480021158.01.T01:three_prime_UTR
TCTTCAGAAGCCGGACTTCC+CGG	0.331048	4:-54528793	MsG0480021158.01.T01:CDS
ATGGTGGCTTGGAGGAAATT+TGG	0.335560	4:-54526575	MsG0480021158.01.T01:three_prime_UTR
TAGCAATGCTTCTGAGGTTA+AGG	0.340241	4:-54529972	MsG0480021158.01.T01:CDS
TGATTCTCCAACACTTTCAT+TGG	0.340325	4:+54529917	None:intergenic
AGTTCCTTGTAAGGCTTGTT+TGG	0.347179	4:+54527091	None:intergenic
AAAATGGCAAATCTAGTTCC+TGG	0.348238	4:-54530355	MsG0480021158.01.T01:CDS
TTACTCTCGTAATCTTTAGA+AGG	0.353943	4:+54528946	None:intergenic
CACTTTCATTGGAGAGGACT+TGG	0.357255	4:+54529928	None:intergenic
TCCACTCTTGGAGTGGTTGT+TGG	0.358763	4:+54527021	None:intergenic
TCAATCAGAAATTTAGTTCA+AGG	0.364637	4:-54529581	MsG0480021158.01.T01:CDS
ACGAAGCAAAGGAACTCTTT+TGG	0.370193	4:-54528823	MsG0480021158.01.T01:CDS
GGAGATTTGTTTACAAATCA+AGG	0.381180	4:-54530226	MsG0480021158.01.T01:CDS
ATTAGATCATCATGTTCATC+AGG	0.391695	4:+54530154	None:intergenic
TTCCGATTCTTCGTGGTGTT+AGG	0.393778	4:-54530066	MsG0480021158.01.T01:CDS
CATTTCCTTTAATCCACTCT+TGG	0.393945	4:+54527009	None:intergenic
GGATGAGAGATTGGTGGATA+AGG	0.407044	4:+54527286	None:intergenic
CTTAGTGACAAGATTCAGTT+AGG	0.416620	4:-54530130	MsG0480021158.01.T01:CDS
AACACCAAGTAAGTCCTCTA+AGG	0.416936	4:-54528917	MsG0480021158.01.T01:CDS
GACAAAGTTTCCGGGAAGTC+CGG	0.419326	4:+54528783	None:intergenic
TCTTCCTTAGAGGACTTACT+TGG	0.421315	4:+54528913	None:intergenic
AGTTAACGGTGATGGAACTA+TGG	0.422657	4:-54528863	MsG0480021158.01.T01:CDS
GAATTACTTGTAGTTGAAAC+AGG	0.432215	4:+54527224	None:intergenic
GAGAATTTCTCAAAAGAAGT+AGG	0.433004	4:+54529731	None:intergenic
CTTCTGAGGTTAAGGTTAAG+AGG	0.436051	4:-54529964	MsG0480021158.01.T01:CDS
CCTTCATCTATTGCGAAGCT+TGG	0.438351	4:-54528703	MsG0480021158.01.T01:CDS
CTAAGGCGTTGCGGAAAAGC+TGG	0.438960	4:-54529544	MsG0480021158.01.T01:CDS
AACAGAAAAGGATGAGAGAT+TGG	0.439259	4:+54527277	None:intergenic
TGTTTGGAATTAGCTACTGC+TGG	0.443115	4:+54527107	None:intergenic
GAAACCTTGACAAAGTTTCC+GGG	0.443615	4:+54528775	None:intergenic
TTTGCGCGGAGTTCCTTGTA+AGG	0.448101	4:+54527082	None:intergenic
GGGGTGGTGGAGACGGGAAA+GGG	0.448470	4:-54529650	MsG0480021158.01.T01:CDS
TTAGTTGCAACAATATCTTC+AGG	0.451042	4:+54530010	None:intergenic
ATTCTGAGGGTTATGTTCCT+TGG	0.451057	4:+54527487	None:intergenic
ATAAAGAGACATCCAAATCA+AGG	0.456508	4:-54529496	MsG0480021158.01.T01:CDS
AAATCTATAAAGAAAGTTAA+CGG	0.457905	4:-54528877	MsG0480021158.01.T01:CDS
TGCAATTGCGATCGCGAGAC+TGG	0.462113	4:-54526953	MsG0480021158.01.T01:CDS
CTTCATCTATTGCGAAGCTT+GGG	0.468822	4:-54528702	MsG0480021158.01.T01:CDS
GGAAAGAAATCTTCAGAAGC+CGG	0.471468	4:-54528802	MsG0480021158.01.T01:CDS
GGCCTAACACCACGAAGAAT+CGG	0.472446	4:+54530064	None:intergenic
TTATCTGGAGATGAACCATC+TGG	0.472677	4:+54527386	None:intergenic
AACTTAAAAGCGTCAACGAT+TGG	0.476478	4:-54526845	MsG0480021158.01.T01:CDS
TCGCCTGTTCCGATTCTTCG+TGG	0.478185	4:-54530073	MsG0480021158.01.T01:CDS
CTAAATTAACCGCCGCATCA+AGG	0.478940	4:+54526985	None:intergenic
CAAGCATGTCTCCCAGGAAC+AGG	0.480995	4:+54530043	None:intergenic
AACTGATCAATTGTATTCTG+AGG	0.483206	4:+54527473	None:intergenic
CATCTATTGCGAAGCTTGGG+AGG	0.483470	4:-54528699	MsG0480021158.01.T01:CDS
TGTAGTTGAAACAGGAAGCT+TGG	0.485663	4:+54527232	None:intergenic
CTCTCGGTTATAAACAGAAA+AGG	0.486637	4:+54527265	None:intergenic
GATGCATTATTATGGTGGCT+TGG	0.489089	4:-54526586	MsG0480021158.01.T01:three_prime_UTR
GACGCTTCATTGAAAGCTCA+TGG	0.489464	4:-54527140	MsG0480021158.01.T01:CDS
TAGCAACTAGTAGGTGAAGA+AGG	0.490380	4:+54529761	None:intergenic
TCAAACAATGGTCAAATAGC+AGG	0.495685	4:-54526879	MsG0480021158.01.T01:CDS
GTTTGAGGTATAGACATAGT+TGG	0.496293	4:-54526488	MsG0480021158.01.T01:three_prime_UTR
CTCCAACACTTTCATTGGAG+AGG	0.497923	4:+54529922	None:intergenic
CCAAGCTTCGCAATAGATGA+AGG	0.500292	4:+54528703	None:intergenic
ATGATTGTGAGTCAGAAGTA+AGG	0.502497	4:+54526697	None:intergenic
GTTCGCGGAGTTAGTCCTCT+CGG	0.502784	4:-54529626	MsG0480021158.01.T01:CDS
TTCTGGTGTTCCAGACTCCT+AGG	0.504156	4:-54528993	MsG0480021158.01.T01:intron
TGACAGAAGCAAGTGTTCAA+TGG	0.504746	4:-54528735	MsG0480021158.01.T01:CDS
GGATAGGCTGATTGCTAAGG+TGG	0.505127	4:-54529672	MsG0480021158.01.T01:CDS
AAATCTCCACCTGCTAGTGC+TGG	0.505213	4:+54530241	None:intergenic
GGCAAAGAATAGCAACTAGT+AGG	0.507800	4:+54529752	None:intergenic
AAATGGCAAATCTAGTTCCT+GGG	0.508006	4:-54530354	MsG0480021158.01.T01:CDS
CCTTTAATCCACTCTTGGAG+TGG	0.512337	4:+54527014	None:intergenic
CTAACTGAATCTTGTCACTA+AGG	0.514275	4:+54530131	None:intergenic
GCACAGAGATGCAGCACAGA+TGG	0.515070	4:-54528176	MsG0480021158.01.T01:CDS
TTAGTTCAAGGAATTGAACT+TGG	0.515121	4:-54529569	MsG0480021158.01.T01:CDS
TAAAAGCGTCAACGATTGGT+TGG	0.515601	4:-54526841	MsG0480021158.01.T01:CDS
AAGCAACAAGGAAATATCAA+TGG	0.517246	4:-54529698	MsG0480021158.01.T01:CDS
ATAAAGAAAGTTAACGGTGA+TGG	0.518959	4:-54528871	MsG0480021158.01.T01:CDS
ACACCACGAAGAATCGGAAC+AGG	0.519947	4:+54530070	None:intergenic
ATAAATCCTACATGGGTTTG+AGG	0.521514	4:-54526503	MsG0480021158.01.T01:three_prime_UTR
TTGGACAGTGATGGAGACAT+TGG	0.524679	4:-54526906	MsG0480021158.01.T01:CDS
AATTCCAAACAAGCCTTACA+AGG	0.526341	4:-54527095	MsG0480021158.01.T01:CDS
GAGACGGGAAAGGGTGTTCG+CGG	0.527317	4:-54529641	MsG0480021158.01.T01:CDS
AGCATTGTTCCAGCACTAGC+AGG	0.527702	4:-54530250	MsG0480021158.01.T01:CDS
AGGAATTGAACTTGGTGCTA+AGG	0.528575	4:-54529561	MsG0480021158.01.T01:CDS
CTTAAGATGCATTATTATGG+TGG	0.529334	4:-54526591	MsG0480021158.01.T01:three_prime_UTR
AAGAAAAGACAACGAAGCAA+AGG	0.535441	4:-54528834	MsG0480021158.01.T01:CDS
AGTCTATCAATTGTCTCTAC+CGG	0.535694	4:+54526774	None:intergenic
TTCGCGGAGTTAGTCCTCTC+GGG	0.536295	4:-54529625	MsG0480021158.01.T01:CDS
TCTATACCTCAAACCCATGT+AGG	0.541148	4:+54526497	None:intergenic
ACAAGTAGATAAGAAATCAA+TGG	0.541333	4:-54529888	MsG0480021158.01.T01:CDS
CAAGGAACTCCGCGCAAACA+AGG	0.543841	4:-54527077	MsG0480021158.01.T01:CDS
AACGATTGGTTGGATGAGAT+AGG	0.546451	4:-54526831	MsG0480021158.01.T01:CDS
CCTGGGAGACATGCTTGTGT+TGG	0.547891	4:-54530037	MsG0480021158.01.T01:CDS
GCATTATTATGGTGGCTTGG+AGG	0.549963	4:-54526583	MsG0480021158.01.T01:three_prime_UTR
ATGAACACAGACATAAAAGT+TGG	0.550757	4:-54530307	MsG0480021158.01.T01:CDS
GAAAAGCTGGGAAGGGAACA+TGG	0.551231	4:-54529531	MsG0480021158.01.T01:CDS
ACTGATCAATTGTATTCTGA+GGG	0.552570	4:+54527474	None:intergenic
GTGGTGTTAGGCCTGTTCCT+GGG	0.553445	4:-54530054	MsG0480021158.01.T01:CDS
ATTGTTGCAACTAACTCACT+TGG	0.554897	4:-54530001	MsG0480021158.01.T01:CDS
AGAAAAGGATGAGAGATTGG+TGG	0.555800	4:+54527280	None:intergenic
GAAATATCAATGGAGTGGAT+AGG	0.557954	4:-54529688	MsG0480021158.01.T01:CDS
GTCCTCTCCAATGAAAGTGT+TGG	0.558109	4:-54529924	MsG0480021158.01.T01:CDS
CTTTCTAAAACCATTCCAGA+TGG	0.563456	4:-54527401	MsG0480021158.01.T01:CDS
ACAGGAAGCTTGGACAATTG+AGG	0.563685	4:+54527242	None:intergenic
GCTTGGACAATTGAGGCTCT+CGG	0.566507	4:+54527249	None:intergenic
ATCTATTGCGAAGCTTGGGA+GGG	0.567095	4:-54528698	MsG0480021158.01.T01:intron
GACATTGGTTTGTCAAACAA+TGG	0.568828	4:-54526891	MsG0480021158.01.T01:CDS
GAGTTCTGATTATTTATTTG+GGG	0.570661	4:-54526466	MsG0480021158.01.T01:three_prime_UTR
ATAGGCTGATTGCTAAGGTG+GGG	0.576897	4:-54529670	MsG0480021158.01.T01:CDS
TAGGCTGATTGCTAAGGTGG+GGG	0.578325	4:-54529669	MsG0480021158.01.T01:CDS
TCCAACAACCACTCCAAGAG+TGG	0.580092	4:-54527022	MsG0480021158.01.T01:CDS
TGGAGATGAACCATCTGGAA+TGG	0.580483	4:+54527391	None:intergenic
TTTGTACATAAATCCTACAT+GGG	0.580524	4:-54526510	MsG0480021158.01.T01:three_prime_UTR
GATTGGTGGATAAGGCAGCT+TGG	0.581530	4:+54527294	None:intergenic
CTCGGGAAGAGAATTGCAGT+TGG	0.583302	4:-54529608	MsG0480021158.01.T01:CDS
CCAACACAAGCATGTCTCCC+AGG	0.583666	4:+54530037	None:intergenic
AACAATAAATGTGAAATGTG+AGG	0.588377	4:-54526442	MsG0480021158.01.T01:three_prime_UTR
TAGAGACAATTGATAGACTC+AGG	0.589019	4:-54526770	MsG0480021158.01.T01:CDS
AACTTGGTGCTAAGGCGTTG+CGG	0.593967	4:-54529553	MsG0480021158.01.T01:CDS
TAAAGAGACATCCAAATCAA+GGG	0.597215	4:-54529495	MsG0480021158.01.T01:CDS
ACAAGGAAATATCAATGGAG+TGG	0.598845	4:-54529693	MsG0480021158.01.T01:CDS
AACACAGACATAAAAGTTGG+TGG	0.601218	4:-54530304	MsG0480021158.01.T01:CDS
GCGTTGCGGAAAAGCTGGGA+AGG	0.605950	4:-54529539	MsG0480021158.01.T01:CDS
AGCAACTGAGGCTATGCAAG+AGG	0.606268	4:-54528152	MsG0480021158.01.T01:CDS
TGATTGTGAGTCAGAAGTAA+GGG	0.607233	4:+54526698	None:intergenic
ATTAACTTTAGTAGCACCCC+AGG	0.608080	4:+54530337	None:intergenic
AGGAAATGACCTTGATGCGG+CGG	0.609502	4:-54526994	MsG0480021158.01.T01:CDS
CGATCGCAATTGCAGCATGT+CGG	0.611032	4:+54526962	None:intergenic
ATTGCATCACCTTGTTTGCG+CGG	0.616347	4:+54527068	None:intergenic
TCACAATCATCAGAGATCAA+AGG	0.618878	4:-54526684	MsG0480021158.01.T01:CDS
AGCACAGATGGCAGCAACTG+AGG	0.619137	4:-54528164	MsG0480021158.01.T01:CDS
ACTTCCCGGAAACTTTGTCA+AGG	0.621117	4:-54528779	MsG0480021158.01.T01:CDS
TAAGGCGTTGCGGAAAAGCT+GGG	0.622391	4:-54529543	MsG0480021158.01.T01:CDS
GATAGGCTGATTGCTAAGGT+GGG	0.622398	4:-54529671	MsG0480021158.01.T01:CDS
CAAGCTTCGCAATAGATGAA+GGG	0.632626	4:+54528704	None:intergenic
CGTTGCGGAAAAGCTGGGAA+GGG	0.647668	4:-54529538	MsG0480021158.01.T01:CDS
AGAGCTAAGTAATTCTGCCA+AGG	0.648404	4:-54527504	MsG0480021158.01.T01:CDS
AGTGGATAGGCTGATTGCTA+AGG	0.655516	4:-54529675	MsG0480021158.01.T01:CDS
TCTAATGGAGTTAAGCAACA+AGG	0.656865	4:-54529710	MsG0480021158.01.T01:CDS
AATGGCAAATCTAGTTCCTG+GGG	0.665627	4:-54530353	MsG0480021158.01.T01:CDS
TTAGATCATCATGTTCATCA+GGG	0.666720	4:+54530155	None:intergenic
TAAAGGAAATGACCTTGATG+CGG	0.678002	4:-54526997	MsG0480021158.01.T01:CDS
ACACTAGCCTGAATAGTGCG+CGG	0.681954	4:-54527439	MsG0480021158.01.T01:CDS
ATTGTTCCAGCACTAGCAGG+TGG	0.697945	4:-54530247	MsG0480021158.01.T01:CDS
ACTGCAATTCTCTTCCCGAG+AGG	0.722826	4:+54529611	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GAATTTTGTTTTTTTTTTTT+TGG	+	Chr4:54530185-54530204	None:intergenic	10.0%
!!	AAATCTATAAAGAAAGTTAA+CGG	-	Chr4:54527854-54527873	MsG0480021158.01.T01:intron	15.0%
!!	ATTTATGTACAAAAAAAAAG+TGG	+	Chr4:54530214-54530233	None:intergenic	15.0%
!!	TAAAATAAGTAAATACAAAC+AGG	+	Chr4:54527325-54527344	None:intergenic	15.0%
!!	AAGATATGCATTACTAATAA+AGG	-	Chr4:54528735-54528754	MsG0480021158.01.T01:CDS	20.0%
!!	AAGTATCAACATTATATCTT+CGG	+	Chr4:54528840-54528859	None:intergenic	20.0%
!!	AGTAAGTTTATTCACAATTT+AGG	-	Chr4:54527299-54527318	MsG0480021158.01.T01:CDS	20.0%
!!	CATACAACATTCATATTATT+AGG	-	Chr4:54528361-54528380	MsG0480021158.01.T01:intron	20.0%
!!	GCATACAATAACTTTATAAA+AGG	+	Chr4:54530330-54530349	None:intergenic	20.0%
!!	TATTGTAATCTAATCAATGT+AGG	-	Chr4:54528937-54528956	MsG0480021158.01.T01:CDS	20.0%
!!	TTTATTAGTAATGCATATCT+TGG	+	Chr4:54528736-54528755	None:intergenic	20.0%
!!!	ATTGTTTGTTGAGAAATTTT+TGG	-	Chr4:54529806-54529825	MsG0480021158.01.T01:CDS	20.0%
!!!	TTTTTTAAAGAAATTGGGTA+AGG	+	Chr4:54529108-54529127	None:intergenic	20.0%
!	AACAATAAATGTGAAATGTG+AGG	-	Chr4:54530289-54530308	MsG0480021158.01.T01:CDS	25.0%
!	ACAAGTAGATAAGAAATCAA+TGG	-	Chr4:54526843-54526862	MsG0480021158.01.T01:CDS	25.0%
!	CAAAATCAAAAGATTGTTCT+TGG	-	Chr4:54529531-54529550	MsG0480021158.01.T01:CDS	25.0%
!	CATAAACCTTAAGTCAATAA+CGG	+	Chr4:54528420-54528439	None:intergenic	25.0%
!	GTATACAACATTCATATTGT+TGG	-	Chr4:54528393-54528412	MsG0480021158.01.T01:intron	25.0%
!	TAATGAATAATGAATCCAGT+AGG	+	Chr4:54528457-54528476	None:intergenic	25.0%
!	TAGCTTAAGATGCATTATTA+TGG	-	Chr4:54530137-54530156	MsG0480021158.01.T01:CDS	25.0%
!	TAGTTGTATCATTAGTTAGT+TGG	-	Chr4:54528107-54528126	MsG0480021158.01.T01:intron	25.0%
!	TATAAGAAGTGATTATGCAT+AGG	+	Chr4:54527494-54527513	None:intergenic	25.0%
!	TGAAACATATAGTGAAATTC+AGG	-	Chr4:54528968-54528987	MsG0480021158.01.T01:CDS	25.0%
!	TTTGTACATAAATCCTACAT+GGG	-	Chr4:54530221-54530240	MsG0480021158.01.T01:CDS	25.0%
!!	AAATCAAGGCTTTTATGTTA+AGG	-	Chr4:54526519-54526538	MsG0480021158.01.T01:three_prime_UTR	25.0%
!!	AAGATATTTCTGACACTATT+TGG	-	Chr4:54529181-54529200	MsG0480021158.01.T01:intron	25.0%
!!	AGCATTAAATACTTTTGTTG+GGG	+	Chr4:54528156-54528175	None:intergenic	25.0%
!!	GAGCATTAAATACTTTTGTT+GGG	+	Chr4:54528157-54528176	None:intergenic	25.0%
!!	GAGTTCTGATTATTTATTTG+GGG	-	Chr4:54530265-54530284	MsG0480021158.01.T01:CDS	25.0%
!!	GATAAGAAATCAATGGTTTT+AGG	-	Chr4:54526850-54526869	MsG0480021158.01.T01:CDS	25.0%
!!	GGAGTTCTGATTATTTATTT+GGG	-	Chr4:54530264-54530283	MsG0480021158.01.T01:CDS	25.0%
!!	TCAATCAGAAATTTAGTTCA+AGG	-	Chr4:54527150-54527169	MsG0480021158.01.T01:CDS	25.0%
!!	TGGAGTTCTGATTATTTATT+TGG	-	Chr4:54530263-54530282	MsG0480021158.01.T01:CDS	25.0%
!!	TTAAAACAAATCACCTTTTG+TGG	-	Chr4:54528782-54528801	MsG0480021158.01.T01:CDS	25.0%
!!	TTTTAACAAGATTCACTTTC+TGG	-	Chr4:54527721-54527740	MsG0480021158.01.T01:intron	25.0%
!!	TTTTGTACATAAATCCTACA+TGG	-	Chr4:54530220-54530239	MsG0480021158.01.T01:CDS	25.0%
!!!	AGACGATTTTAAGTTGTTTA+TGG	-	Chr4:54528895-54528914	MsG0480021158.01.T01:CDS	25.0%
!!!	ATTTTTTGCATATCCACAAA+AGG	+	Chr4:54528798-54528817	None:intergenic	25.0%
!!!	TCTTTTGAGAAATTCTCTAA+TGG	-	Chr4:54527006-54527025	MsG0480021158.01.T01:CDS	25.0%
!!!	TTTTTTTTTCACATCTTGAC+AGG	-	Chr4:54528522-54528541	MsG0480021158.01.T01:intron	25.0%
	AAACAAAGAAAAGTCCAACA+AGG	+	Chr4:54527620-54527639	None:intergenic	30.0%
	AACTGATCAATTGTATTCTG+AGG	+	Chr4:54529261-54529280	None:intergenic	30.0%
	AAGCAACAAGGAAATATCAA+TGG	-	Chr4:54527033-54527052	MsG0480021158.01.T01:CDS	30.0%
	AAGCTGAAACTGCAAAAATA+AGG	+	Chr4:54528078-54528097	None:intergenic	30.0%
	AATTCAAACTCTGTCTCTTT+AGG	+	Chr4:54529027-54529046	None:intergenic	30.0%
	ACTGATCAATTGTATTCTGA+GGG	+	Chr4:54529260-54529279	None:intergenic	30.0%
	ATAAAGAAAGTTAACGGTGA+TGG	-	Chr4:54527860-54527879	MsG0480021158.01.T01:intron	30.0%
	ATAAAGAGACATCCAAATCA+AGG	-	Chr4:54527235-54527254	MsG0480021158.01.T01:CDS	30.0%
	ATAGGATCGAGTAAAAATGA+GGG	-	Chr4:54529918-54529937	MsG0480021158.01.T01:CDS	30.0%
	ATGAACACAGACATAAAAGT+TGG	-	Chr4:54526424-54526443	MsG0480021158.01.T01:three_prime_UTR	30.0%
	ATTAGATCATCATGTTCATC+AGG	+	Chr4:54526580-54526599	None:intergenic	30.0%
	CAACATTATATCTTCGGTAT+CGG	+	Chr4:54528834-54528853	None:intergenic	30.0%
	CTTAACCGAGTTAAAAAACA+TGG	+	Chr4:54528229-54528248	None:intergenic	30.0%
	CTTAAGATGCATTATTATGG+TGG	-	Chr4:54530140-54530159	MsG0480021158.01.T01:CDS	30.0%
	CTTAATTGATACGTTTATCC+AGG	-	Chr4:54527556-54527575	MsG0480021158.01.T01:intron	30.0%
	CTTATCATTGATGAAACATG+TGG	-	Chr4:54528857-54528876	MsG0480021158.01.T01:CDS	30.0%
	GAATTACTTGTAGTTGAAAC+AGG	+	Chr4:54529510-54529529	None:intergenic	30.0%
	GAGAATTTCTCAAAAGAAGT+AGG	+	Chr4:54527003-54527022	None:intergenic	30.0%
	GATCTTTGTGTTTCTTAATC+TGG	-	Chr4:54528661-54528680	MsG0480021158.01.T01:intron	30.0%
	GGAAGATGTTTAAACATCTA+AGG	+	Chr4:54529148-54529167	None:intergenic	30.0%
	GTTGTGATTCTTTCATTATC+TGG	+	Chr4:54529363-54529382	None:intergenic	30.0%
	TAAAGAGACATCCAAATCAA+GGG	-	Chr4:54527236-54527255	MsG0480021158.01.T01:CDS	30.0%
	TCAAAGTTGTTCAAAAAGAC+AGG	+	Chr4:54527582-54527601	None:intergenic	30.0%
	TTACTCTCGTAATCTTTAGA+AGG	+	Chr4:54527788-54527807	None:intergenic	30.0%
	TTAGATCATCATGTTCATCA+GGG	+	Chr4:54526579-54526598	None:intergenic	30.0%
	TTAGTTCAAGGAATTGAACT+TGG	-	Chr4:54527162-54527181	MsG0480021158.01.T01:CDS	30.0%
	TTAGTTGCAACAATATCTTC+AGG	+	Chr4:54526724-54526743	None:intergenic	30.0%
!	ATTATGCATAGGCTATTTCA+TGG	+	Chr4:54527483-54527502	None:intergenic	30.0%
!	CGAGCATTAAATACTTTTGT+TGG	+	Chr4:54528158-54528177	None:intergenic	30.0%
!	GCATGACAATTGATTCAAAT+GGG	+	Chr4:54528311-54528330	None:intergenic	30.0%
!	GGAGATTTGTTTACAAATCA+AGG	-	Chr4:54526505-54526524	MsG0480021158.01.T01:three_prime_UTR	30.0%
!	TCTTAATCTGGTTGAGATTT+TGG	-	Chr4:54528673-54528692	MsG0480021158.01.T01:intron	30.0%
!	TGCATGACAATTGATTCAAA+TGG	+	Chr4:54528312-54528331	None:intergenic	30.0%
!!	ATAGTTTGCTACAATGTCTT+AGG	-	Chr4:54528614-54528633	MsG0480021158.01.T01:intron	30.0%
!!	CTTATTTCTATAAGCACTCT+AGG	-	Chr4:54527430-54527449	MsG0480021158.01.T01:CDS	30.0%
!!	GAATTTTCGAGGACTAAAAT+TGG	+	Chr4:54529564-54529583	None:intergenic	30.0%
!!!	AATCTTGTTTTCTTCCTTAG+AGG	+	Chr4:54527831-54527850	None:intergenic	30.0%
!!!	ATCTGGAATGGTTTTAGAAA+GGG	+	Chr4:54529331-54529350	None:intergenic	30.0%
!!!	CATTGCCATGTTTTTTAACT+CGG	-	Chr4:54528221-54528240	MsG0480021158.01.T01:intron	30.0%
!!!	TGTTGTTGATTTTGTGAAGA+AGG	-	Chr4:54526903-54526922	MsG0480021158.01.T01:CDS	30.0%
	AACACAGACATAAAAGTTGG+TGG	-	Chr4:54526427-54526446	MsG0480021158.01.T01:three_prime_UTR	35.0%
	AACAGAAAAGGATGAGAGAT+TGG	+	Chr4:54529457-54529476	None:intergenic	35.0%
	AAGAAAAGACAACGAAGCAA+AGG	-	Chr4:54527897-54527916	MsG0480021158.01.T01:intron	35.0%
	AATTCCAAACAAGCCTTACA+AGG	-	Chr4:54529636-54529655	MsG0480021158.01.T01:CDS	35.0%
	ACAAAAACTTGCAGCTTCTT+GGG	-	Chr4:54529413-54529432	MsG0480021158.01.T01:intron	35.0%
	ACAAGGAAATATCAATGGAG+TGG	-	Chr4:54527038-54527057	MsG0480021158.01.T01:CDS	35.0%
	ACCGATCAACAAAAACAAAC+TGG	+	Chr4:54526627-54526646	None:intergenic	35.0%
	AGTCTATCAATTGTCTCTAC+CGG	+	Chr4:54529960-54529979	None:intergenic	35.0%
	AGTTTGAATTTCCTCAGGAA+CGG	-	Chr4:54529037-54529056	MsG0480021158.01.T01:intron	35.0%
	ATAAATCCTACATGGGTTTG+AGG	-	Chr4:54530228-54530247	MsG0480021158.01.T01:CDS	35.0%
	ATGATTGTGAGTCAGAAGTA+AGG	+	Chr4:54530037-54530056	None:intergenic	35.0%
	ATTGTTGCAACTAACTCACT+TGG	-	Chr4:54526730-54526749	MsG0480021158.01.T01:CDS	35.0%
	CATTTCCTTTAATCCACTCT+TGG	+	Chr4:54529725-54529744	None:intergenic	35.0%
	CTAACTGAATCTTGTCACTA+AGG	+	Chr4:54526603-54526622	None:intergenic	35.0%
	CTCTCGGTTATAAACAGAAA+AGG	+	Chr4:54529469-54529488	None:intergenic	35.0%
	CTTAGTGACAAGATTCAGTT+AGG	-	Chr4:54526601-54526620	MsG0480021158.01.T01:three_prime_UTR	35.0%
	GAAATATCAATGGAGTGGAT+AGG	-	Chr4:54527043-54527062	MsG0480021158.01.T01:CDS	35.0%
	GATAGGATCGAGTAAAAATG+AGG	-	Chr4:54529917-54529936	MsG0480021158.01.T01:CDS	35.0%
	GTCAGAAATATCTTGAGTAG+TGG	+	Chr4:54529175-54529194	None:intergenic	35.0%
	TAAAGGAAATGACCTTGATG+CGG	-	Chr4:54529734-54529753	MsG0480021158.01.T01:CDS	35.0%
	TAGAGACAATTGATAGACTC+AGG	-	Chr4:54529961-54529980	MsG0480021158.01.T01:CDS	35.0%
	TAGGATCGAGTAAAAATGAG+GGG	-	Chr4:54529919-54529938	MsG0480021158.01.T01:CDS	35.0%
	TCAAACAATGGTCAAATAGC+AGG	-	Chr4:54529852-54529871	MsG0480021158.01.T01:CDS	35.0%
	TCACAATCATCAGAGATCAA+AGG	-	Chr4:54530047-54530066	MsG0480021158.01.T01:CDS	35.0%
	TCTAATGGAGTTAAGCAACA+AGG	-	Chr4:54527021-54527040	MsG0480021158.01.T01:CDS	35.0%
	TGAAACCTTGACAAAGTTTC+CGG	+	Chr4:54527960-54527979	None:intergenic	35.0%
	TGATTCTCCAACACTTTCAT+TGG	+	Chr4:54526817-54526836	None:intergenic	35.0%
	TGATTGTGAGTCAGAAGTAA+GGG	+	Chr4:54530036-54530055	None:intergenic	35.0%
!	AAAATGGCAAATCTAGTTCC+TGG	-	Chr4:54526376-54526395	MsG0480021158.01.T01:three_prime_UTR	35.0%
!	AAATGGCAAATCTAGTTCCT+GGG	-	Chr4:54526377-54526396	MsG0480021158.01.T01:three_prime_UTR	35.0%
!	CTCTGTCTCTTTAGGTTATA+AGG	+	Chr4:54529019-54529038	None:intergenic	35.0%
!	GAGTTTGAGATAATACCTAC+TGG	-	Chr4:54528439-54528458	MsG0480021158.01.T01:intron	35.0%
!	GTTTGAGGTATAGACATAGT+TGG	-	Chr4:54530243-54530262	MsG0480021158.01.T01:CDS	35.0%
!	TTTTCTTAGCAATGCTTCTG+AGG	-	Chr4:54526753-54526772	MsG0480021158.01.T01:CDS	35.0%
!!	AACTTAAAAGCGTCAACGAT+TGG	-	Chr4:54529886-54529905	MsG0480021158.01.T01:CDS	35.0%
!!	CTTTCTAAAACCATTCCAGA+TGG	-	Chr4:54529330-54529349	MsG0480021158.01.T01:intron	35.0%
!!	GACATTGGTTTGTCAAACAA+TGG	-	Chr4:54529840-54529859	MsG0480021158.01.T01:CDS	35.0%
!!	GAGAAATTTTTGGACAGTGA+TGG	-	Chr4:54529816-54529835	MsG0480021158.01.T01:CDS	35.0%
!!!	CATCTGGAATGGTTTTAGAA+AGG	+	Chr4:54529332-54529351	None:intergenic	35.0%
!!!	GCCAGTTTGTTTTTGTTGAT+CGG	-	Chr4:54526623-54526642	MsG0480021158.01.T01:three_prime_UTR	35.0%
!!!	TGTTGGACTAGTTGCTTTTT+TGG	+	Chr4:54529696-54529715	None:intergenic	35.0%
	AACACCAAGTAAGTCCTCTA+AGG	-	Chr4:54527814-54527833	MsG0480021158.01.T01:intron	40.0%
	AAGAAAAGTCCAACAAGGTC+TGG	+	Chr4:54527615-54527634	None:intergenic	40.0%
	AATATCTTGAGTAGTGGCAG+AGG	+	Chr4:54529169-54529188	None:intergenic	40.0%
	AGAAAAGGATGAGAGATTGG+TGG	+	Chr4:54529454-54529473	None:intergenic	40.0%
	AGACAGTTTGGTAGAGCTTA+TGG	-	Chr4:54527372-54527391	MsG0480021158.01.T01:CDS	40.0%
	AGAGCTAAGTAATTCTGCCA+AGG	-	Chr4:54529227-54529246	MsG0480021158.01.T01:intron	40.0%
	AGGATCGAGTAAAAATGAGG+GGG	-	Chr4:54529920-54529939	MsG0480021158.01.T01:CDS	40.0%
	ATTAACTTTAGTAGCACCCC+AGG	+	Chr4:54526397-54526416	None:intergenic	40.0%
	CAAGCTTCGCAATAGATGAA+GGG	+	Chr4:54528030-54528049	None:intergenic	40.0%
	CTTACTGTGCTACGAAATTC+AGG	+	Chr4:54527285-54527304	None:intergenic	40.0%
	CTTAGAAGATGCCCTTGATT+TGG	+	Chr4:54527250-54527269	None:intergenic	40.0%
	CTTCATCTATTGCGAAGCTT+GGG	-	Chr4:54528029-54528048	MsG0480021158.01.T01:intron	40.0%
	GAAACCTTGACAAAGTTTCC+GGG	+	Chr4:54527959-54527978	None:intergenic	40.0%
	GACAAAAACTTGCAGCTTCT+TGG	-	Chr4:54529412-54529431	MsG0480021158.01.T01:intron	40.0%
	GACAGAGTTTGAATTTCCTC+AGG	-	Chr4:54529032-54529051	MsG0480021158.01.T01:intron	40.0%
	GATGATGAGTCATAACAGAC+AGG	+	Chr4:54528274-54528293	None:intergenic	40.0%
	GATGCATTATTATGGTGGCT+TGG	-	Chr4:54530145-54530164	MsG0480021158.01.T01:CDS	40.0%
	GGAAAGAAATCTTCAGAAGC+CGG	-	Chr4:54527929-54527948	MsG0480021158.01.T01:intron	40.0%
	GGCAAAGAATAGCAACTAGT+AGG	+	Chr4:54526982-54527001	None:intergenic	40.0%
	GTCATAACAGACAGGATCTT+TGG	+	Chr4:54528266-54528285	None:intergenic	40.0%
	TAAGCACTTGTGAGACAGTT+TGG	-	Chr4:54527360-54527379	MsG0480021158.01.T01:CDS	40.0%
	TAGCAACTAGTAGGTGAAGA+AGG	+	Chr4:54526973-54526992	None:intergenic	40.0%
	TCTATACCTCAAACCCATGT+AGG	+	Chr4:54530237-54530256	None:intergenic	40.0%
	TCTTCCTTAGAGGACTTACT+TGG	+	Chr4:54527821-54527840	None:intergenic	40.0%
	TGACAGAAGCAAGTGTTCAA+TGG	-	Chr4:54527996-54528015	MsG0480021158.01.T01:intron	40.0%
	TGTAGTTGAAACAGGAAGCT+TGG	+	Chr4:54529502-54529521	None:intergenic	40.0%
	TTATCTGGAGATGAACCATC+TGG	+	Chr4:54529348-54529367	None:intergenic	40.0%
!	AACGATTGGTTGGATGAGAT+AGG	-	Chr4:54529900-54529919	MsG0480021158.01.T01:CDS	40.0%
!	AATGGCAAATCTAGTTCCTG+GGG	-	Chr4:54526378-54526397	MsG0480021158.01.T01:three_prime_UTR	40.0%
!	ACGAAGCAAAGGAACTCTTT+TGG	-	Chr4:54527908-54527927	MsG0480021158.01.T01:intron	40.0%
!	AGTTCCTTGTAAGGCTTGTT+TGG	+	Chr4:54529643-54529662	None:intergenic	40.0%
!	ATGTACTTTTCCTAGGAGTC+TGG	+	Chr4:54527751-54527770	None:intergenic	40.0%
!	ATTCTGAGGGTTATGTTCCT+TGG	+	Chr4:54529247-54529266	None:intergenic	40.0%
!	CGTCTTCTCTTGAATTTTCG+AGG	+	Chr4:54529575-54529594	None:intergenic	40.0%
!	TCACTCGATGTACTTTTCCT+AGG	+	Chr4:54527758-54527777	None:intergenic	40.0%
!	TGTTTGGAATTAGCTACTGC+TGG	+	Chr4:54529627-54529646	None:intergenic	40.0%
!!	AGGAATTGAACTTGGTGCTA+AGG	-	Chr4:54527170-54527189	MsG0480021158.01.T01:CDS	40.0%
!!	AGTTAACGGTGATGGAACTA+TGG	-	Chr4:54527868-54527887	MsG0480021158.01.T01:intron	40.0%
!!	CTTCTGAGGTTAAGGTTAAG+AGG	-	Chr4:54526767-54526786	MsG0480021158.01.T01:CDS	40.0%
!!	GTTGGACCGTTATTGACTTA+AGG	-	Chr4:54528411-54528430	MsG0480021158.01.T01:intron	40.0%
!!	TAAAAGCGTCAACGATTGGT+TGG	-	Chr4:54529890-54529909	MsG0480021158.01.T01:CDS	40.0%
!!	TAGCAATGCTTCTGAGGTTA+AGG	-	Chr4:54526759-54526778	MsG0480021158.01.T01:CDS	40.0%
	AAATCATCCGCGCACTATTC+AGG	+	Chr4:54529302-54529321	None:intergenic	45.0%
	ACAGGAAGCTTGGACAATTG+AGG	+	Chr4:54529492-54529511	None:intergenic	45.0%
	ACTTCCCGGAAACTTTGTCA+AGG	-	Chr4:54527952-54527971	MsG0480021158.01.T01:intron	45.0%
	AGGTCAGACTCTTACTACTG+AGG	+	Chr4:54528999-54529018	None:intergenic	45.0%
	AGTGGATAGGCTGATTGCTA+AGG	-	Chr4:54527056-54527075	MsG0480021158.01.T01:CDS	45.0%
	ATAGGCTGATTGCTAAGGTG+GGG	-	Chr4:54527061-54527080	MsG0480021158.01.T01:CDS	45.0%
	ATCTATTGCGAAGCTTGGGA+GGG	-	Chr4:54528033-54528052	MsG0480021158.01.T01:intron	45.0%
	CACTTTCATTGGAGAGGACT+TGG	+	Chr4:54526806-54526825	None:intergenic	45.0%
	CCAAGCTTCGCAATAGATGA+AGG	+	Chr4:54528031-54528050	None:intergenic	45.0%
	CCTCAGGAACGGTTGTAAAA+TGG	-	Chr4:54529048-54529067	MsG0480021158.01.T01:intron	45.0%
	CCTTCATCTATTGCGAAGCT+TGG	-	Chr4:54528028-54528047	MsG0480021158.01.T01:intron	45.0%
	CCTTTAATCCACTCTTGGAG+TGG	+	Chr4:54529720-54529739	None:intergenic	45.0%
	CTAAATTAACCGCCGCATCA+AGG	+	Chr4:54529749-54529768	None:intergenic	45.0%
	CTCCAACACTTTCATTGGAG+AGG	+	Chr4:54526812-54526831	None:intergenic	45.0%
	GACGCTTCATTGAAAGCTCA+TGG	-	Chr4:54529591-54529610	MsG0480021158.01.T01:CDS	45.0%
	GATAGGCTGATTGCTAAGGT+GGG	-	Chr4:54527060-54527079	MsG0480021158.01.T01:CDS	45.0%
	GTTGTTCAAAAAGACAGGCC+TGG	+	Chr4:54527577-54527596	None:intergenic	45.0%
	TGGAGATGAACCATCTGGAA+TGG	+	Chr4:54529343-54529362	None:intergenic	45.0%
	TGTCAAACACCAGACCTTGT+TGG	-	Chr4:54527603-54527622	MsG0480021158.01.T01:intron	45.0%
	TTCCGATTCTTCGTGGTGTT+AGG	-	Chr4:54526665-54526684	MsG0480021158.01.T01:exon	45.0%
!	ATGGTGGCTTGGAGGAAATT+TGG	-	Chr4:54530156-54530175	MsG0480021158.01.T01:CDS	45.0%
!	ATTGCATCACCTTGTTTGCG+CGG	+	Chr4:54529666-54529685	None:intergenic	45.0%
!	CCATTTTACAACCGTTCCTG+AGG	+	Chr4:54529051-54529070	None:intergenic	45.0%
!	GCATTATTATGGTGGCTTGG+AGG	-	Chr4:54530148-54530167	MsG0480021158.01.T01:CDS	45.0%
!	GGATGAGAGATTGGTGGATA+AGG	+	Chr4:54529448-54529467	None:intergenic	45.0%
!	GTCCTCTCCAATGAAAGTGT+TGG	-	Chr4:54526807-54526826	MsG0480021158.01.T01:CDS	45.0%
!	TTGGACAGTGATGGAGACAT+TGG	-	Chr4:54529825-54529844	MsG0480021158.01.T01:CDS	45.0%
!!	CCACTCCAAGAGTGGATTAA+AGG	-	Chr4:54529717-54529736	MsG0480021158.01.T01:CDS	45.0%
!!!	ATATATTTTTTTAAAGAAAT+TGG	+	Chr4:54529114-54529133	None:intergenic	5.0%
!!!	TATATTTTTTTAAAGAAATT+GGG	+	Chr4:54529113-54529132	None:intergenic	5.0%
	AAATCTCCACCTGCTAGTGC+TGG	+	Chr4:54526493-54526512	None:intergenic	50.0%
	ACACCACGAAGAATCGGAAC+AGG	+	Chr4:54526664-54526683	None:intergenic	50.0%
	ACTGCAATTCTCTTCCCGAG+AGG	+	Chr4:54527123-54527142	None:intergenic	50.0%
	AGCAACTGAGGCTATGCAAG+AGG	-	Chr4:54528579-54528598	MsG0480021158.01.T01:intron	50.0%
	AGCATTGTTCCAGCACTAGC+AGG	-	Chr4:54526481-54526500	MsG0480021158.01.T01:three_prime_UTR	50.0%
	AGGAAATGACCTTGATGCGG+CGG	-	Chr4:54529737-54529756	MsG0480021158.01.T01:CDS	50.0%
	ATTGTTCCAGCACTAGCAGG+TGG	-	Chr4:54526484-54526503	MsG0480021158.01.T01:three_prime_UTR	50.0%
	CATCTATTGCGAAGCTTGGG+AGG	-	Chr4:54528032-54528051	MsG0480021158.01.T01:intron	50.0%
	CGATCGCAATTGCAGCATGT+CGG	+	Chr4:54529772-54529791	None:intergenic	50.0%
	CTCGGGAAGAGAATTGCAGT+TGG	-	Chr4:54527123-54527142	MsG0480021158.01.T01:CDS	50.0%
	GAAAAGCTGGGAAGGGAACA+TGG	-	Chr4:54527200-54527219	MsG0480021158.01.T01:CDS	50.0%
	GACAAAGTTTCCGGGAAGTC+CGG	+	Chr4:54527951-54527970	None:intergenic	50.0%
	GCTTGGACAATTGAGGCTCT+CGG	+	Chr4:54529485-54529504	None:intergenic	50.0%
	GGATAGGCTGATTGCTAAGG+TGG	-	Chr4:54527059-54527078	MsG0480021158.01.T01:CDS	50.0%
	GGCCTAACACCACGAAGAAT+CGG	+	Chr4:54526670-54526689	None:intergenic	50.0%
	GGGGGAATCATGTCAGATAC+CGG	-	Chr4:54529938-54529957	MsG0480021158.01.T01:CDS	50.0%
	TAGGCTGATTGCTAAGGTGG+GGG	-	Chr4:54527062-54527081	MsG0480021158.01.T01:CDS	50.0%
	TCCAACAACCACTCCAAGAG+TGG	-	Chr4:54529709-54529728	MsG0480021158.01.T01:CDS	50.0%
	TCCACTCTTGGAGTGGTTGT+TGG	+	Chr4:54529713-54529732	None:intergenic	50.0%
	TTCTGGTGTTCCAGACTCCT+AGG	-	Chr4:54527738-54527757	MsG0480021158.01.T01:intron	50.0%
	TTTGCGCGGAGTTCCTTGTA+AGG	+	Chr4:54529652-54529671	None:intergenic	50.0%
!	ACACTAGCCTGAATAGTGCG+CGG	-	Chr4:54529292-54529311	MsG0480021158.01.T01:intron	50.0%
!	GATTGGTGGATAAGGCAGCT+TGG	+	Chr4:54529440-54529459	None:intergenic	50.0%
!!	AACTTGGTGCTAAGGCGTTG+CGG	-	Chr4:54527178-54527197	MsG0480021158.01.T01:CDS	50.0%
!!	TAAGGCGTTGCGGAAAAGCT+GGG	-	Chr4:54527188-54527207	MsG0480021158.01.T01:CDS	50.0%
	AGCACAGATGGCAGCAACTG+AGG	-	Chr4:54528567-54528586	MsG0480021158.01.T01:intron	55.0%
	CAAGCATGTCTCCCAGGAAC+AGG	+	Chr4:54526691-54526710	None:intergenic	55.0%
	CAAGGAACTCCGCGCAAACA+AGG	-	Chr4:54529654-54529673	MsG0480021158.01.T01:CDS	55.0%
	CCAACACAAGCATGTCTCCC+AGG	+	Chr4:54526697-54526716	None:intergenic	55.0%
	CCTGGGAGACATGCTTGTGT+TGG	-	Chr4:54526694-54526713	MsG0480021158.01.T01:CDS	55.0%
	CGTTGCGGAAAAGCTGGGAA+GGG	-	Chr4:54527193-54527212	MsG0480021158.01.T01:CDS	55.0%
	GCACAGAGATGCAGCACAGA+TGG	-	Chr4:54528555-54528574	MsG0480021158.01.T01:intron	55.0%
	GTTCGCGGAGTTAGTCCTCT+CGG	-	Chr4:54527105-54527124	MsG0480021158.01.T01:CDS	55.0%
	TCGCCTGTTCCGATTCTTCG+TGG	-	Chr4:54526658-54526677	MsG0480021158.01.T01:exon	55.0%
	TCTTCAGAAGCCGGACTTCC+CGG	-	Chr4:54527938-54527957	MsG0480021158.01.T01:intron	55.0%
	TGCAATTGCGATCGCGAGAC+TGG	-	Chr4:54529778-54529797	MsG0480021158.01.T01:CDS	55.0%
	TTCGCGGAGTTAGTCCTCTC+GGG	-	Chr4:54527106-54527125	MsG0480021158.01.T01:CDS	55.0%
!	GTGGTGTTAGGCCTGTTCCT+GGG	-	Chr4:54526677-54526696	MsG0480021158.01.T01:CDS	55.0%
!!	CTAAGGCGTTGCGGAAAAGC+TGG	-	Chr4:54527187-54527206	MsG0480021158.01.T01:CDS	55.0%
	GATTGCTAAGGTGGGGGTGG+TGG	-	Chr4:54527068-54527087	MsG0480021158.01.T01:CDS	60.0%
	GCGTTGCGGAAAAGCTGGGA+AGG	-	Chr4:54527192-54527211	MsG0480021158.01.T01:CDS	60.0%
	GCTGATTGCTAAGGTGGGGG+TGG	-	Chr4:54527065-54527084	MsG0480021158.01.T01:CDS	60.0%
!	CGTGGTGTTAGGCCTGTTCC+TGG	-	Chr4:54526676-54526695	MsG0480021158.01.T01:CDS	60.0%
!!	GAGACGGGAAAGGGTGTTCG+CGG	-	Chr4:54527090-54527109	MsG0480021158.01.T01:CDS	60.0%
!!	TAAGGTGGGGGTGGTGGAGA+CGG	-	Chr4:54527074-54527093	MsG0480021158.01.T01:CDS	60.0%
!!	AAGGTGGGGGTGGTGGAGAC+GGG	-	Chr4:54527075-54527094	MsG0480021158.01.T01:CDS	65.0%
!!	GGGGTGGTGGAGACGGGAAA+GGG	-	Chr4:54527081-54527100	MsG0480021158.01.T01:CDS	65.0%
!!	GGGGGTGGTGGAGACGGGAA+AGG	-	Chr4:54527080-54527099	MsG0480021158.01.T01:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	54526365	54530388	54526365	ID=MsG0480021158.01;Name=MsG0480021158.01
Chr4	mRNA	54526365	54530388	54526365	ID=MsG0480021158.01.T01;Parent=MsG0480021158.01;Name=MsG0480021158.01.T01;_AED=0.24;_eAED=0.24;_QI=14\|1\|1\|1\|1\|1\|4\|309\|725
Chr4	exon	54526365	54527535	54526365	ID=MsG0480021158.01.T01:exon:8917;Parent=MsG0480021158.01.T01
Chr4	exon	54528118	54528209	54528118	ID=MsG0480021158.01.T01:exon:8916;Parent=MsG0480021158.01.T01
Chr4	exon	54528699	54529001	54528699	ID=MsG0480021158.01.T01:exon:8915;Parent=MsG0480021158.01.T01
Chr4	exon	54529454	54530388	54529454	ID=MsG0480021158.01.T01:exon:8914;Parent=MsG0480021158.01.T01
Chr4	five_prime_UTR	54530375	54530388	54530375	ID=MsG0480021158.01.T01:five_prime_utr;Parent=MsG0480021158.01.T01
Chr4	CDS	54529454	54530374	54529454	ID=MsG0480021158.01.T01:cds;Parent=MsG0480021158.01.T01
Chr4	CDS	54528699	54529001	54528699	ID=MsG0480021158.01.T01:cds;Parent=MsG0480021158.01.T01
Chr4	CDS	54528118	54528209	54528118	ID=MsG0480021158.01.T01:cds;Parent=MsG0480021158.01.T01
Chr4	CDS	54526674	54527535	54526674	ID=MsG0480021158.01.T01:cds;Parent=MsG0480021158.01.T01
Chr4	three_prime_UTR	54526365	54526673	54526365	ID=MsG0480021158.01.T01:three_prime_utr;Parent=MsG0480021158.01.T01

Gene Sequence

>MsG0480021158.01.T01

ATGGCAAATCTAGTTCCTGGGGTGCTACTAAAGTTAATGCAGCACATGAACACAGACATAAAAGTTGGTGGTGAACATAGATCATCATTGTTACAAGTTGTGAGCATTGTTCCAGCACTAGCAGGTGGAGATTTGTTTACAAATCAAGGCTTTTATGTTAAGGTTTCTGATTCATCACATGCTACTTATGTCTCTCTCCCTGATGAACATGATGATCTAATCCTTAGTGACAAGATTCAGTTAGGCCAGTTTGTTTTTGTTGATCGGTTTGAAGCTGCTTCGCCTGTTCCGATTCTTCGTGGTGTTAGGCCTGTTCCTGGGAGACATGCTTGTGTTGGAACACCTGAAGATATTGTTGCAACTAACTCACTTGGTTTTCTTAGCAATGCTTCTGAGGTTAAGGTTAAGAGGAATAGTGTTTGTTCCAAGTCCTCTCCAATGAAAGTGTTGGAGAATCATCAAGAACAAGTAGATAAGAAATCAATGGTTTTAGGTAGATGTAAATCTCAGACAGCAAAAGATTCTGTTGTTGATTTTGTGAAGAAGGAAAAGTTAACAAGATTAAAGTCATTGAATTCAAGAGCTATTGTTCCTTCTTCACCTACTAGTTGCTATTCTTTGCCTACTTCTTTTGAGAAATTCTCTAATGGAGTTAAGCAACAAGGAAATATCAATGGAGTGGATAGGCTGATTGCTAAGGTGGGGGTGGTGGAGACGGGAAAGGGTGTTCGCGGAGTTAGTCCTCTCGGGAAGAGAATTGCAGTTGGAAATTCAATCAGAAATTTAGTTCAAGGAATTGAACTTGGTGCTAAGGCGTTGCGGAAAAGCTGGGAAGGGAACATGGAAGTTAAGAATAATAAAGAGACATCCAAATCAAGGGCATCTTCTAAGTGTGATTCTAAGCCTGAATTTCGTAGCACAACTCCTAGGAAAAGTACATCGAGTGAGAAGTTTCCTTCTAAAGATTACGAGAGTAAGACACAAACACCAAGTAAGTCCTCTAAGGAAGAAAACAAGATTCAAAAATCTATAAAGAAAGTTAACGGTGATGGAACTATGGAAGAACAAGAAAAGACAACGAAGCAAAGGAACTCTTTTGGAAAGAAATCTTCAGAAGCCGGACTTCCCGGAAACTTTGTCAAGGTTTCAGTAAATAGTAAAAAAGTGACAGAAGCAAGTGTTCAATGGACTTCACTCCCTTCATCTATTGCGAAGCTTGGGAGGGAAGTAATGAAGCACAGAGATGCAGCACAGATGGCAGCAACTGAGGCTATGCAAGAGGCTGCTACTGCTGATAGTTTGCTACAATGTCTTAGTGTGTATGCAGAGCTAAGTAATTCTGCCAAGGAACATAACCCTCAGAATACAATTGATCAGTTTTTAACGCTTCACACTAGCCTGAATAGTGCGCGGATGATTTCAGATTCCCTTTCTAAAACCATTCCAGATGGTTCATCTCCAGATAATGAAAGAATCACAACAGAAGAAGCACTAAAACTCAAATCAGATAGACAAAAACTTGCAGCTTCTTGGGTCCAAGCTGCCTTATCCACCAATCTCTCATCCTTTTCTGTTTATAACCGAGAGCCTCAATTGTCCAAGCTTCCTGTTTCAACTACAAGTAATTCTCAAAATCAAAAGATTGTTCTTGGAAGTAAACCAATTTTAGTCCTCGAAAATTCAAGAGAAGACGCTTCATTGAAAGCTCATGGAAAAACTCGTCCAGCAGTAGCTAATTCCAAACAAGCCTTACAAGGAACTCCGCGCAAACAAGGTGATGCAATATCAAATCCAAAAAAGCAACTAGTCCAACAACCACTCCAAGAGTGGATTAAAGGAAATGACCTTGATGCGGCGGTTAATTTAGCCGACATGCTGCAATTGCGATCGCGAGACTGGTTTTTATTGTTTGTTGAGAAATTTTTGGACAGTGATGGAGACATTGGTTTGTCAAACAATGGTCAAATAGCAGGCATGCTCACTCAACTTAAAAGCGTCAACGATTGGTTGGATGAGATAGGATCGAGTAAAAATGAGGGGGAATCATGTCAGATACCGGTAGAGACAATTGATAGACTCAGGAAGAAGATATACGAATATCTTCTTACACATGTTGAATCTGCTGCTGCTGCCCTTACTTCTGACTCACAATCATCAGAGATCAAAGGCAAAAAGTGA

Protein sequence

>MsG0480021158.01.T01

MANLVPGVLLKLMQHMNTDIKVGGEHRSSLLQVVSIVPALAGGDLFTNQGFYVKVSDSSHATYVSLPDEHDDLILSDKIQLGQFVFVDRFEAASPVPILRGVRPVPGRHACVGTPEDIVATNSLGFLSNASEVKVKRNSVCSKSSPMKVLENHQEQVDKKSMVLGRCKSQTAKDSVVDFVKKEKLTRLKSLNSRAIVPSSPTSCYSLPTSFEKFSNGVKQQGNINGVDRLIAKVGVVETGKGVRGVSPLGKRIAVGNSIRNLVQGIELGAKALRKSWEGNMEVKNNKETSKSRASSKCDSKPEFRSTTPRKSTSSEKFPSKDYESKTQTPSKSSKEENKIQKSIKKVNGDGTMEEQEKTTKQRNSFGKKSSEAGLPGNFVKVSVNSKKVTEASVQWTSLPSSIAKLGREVMKHRDAAQMAATEAMQEAATADSLLQCLSVYAELSNSAKEHNPQNTIDQFLTLHTSLNSARMISDSLSKTIPDGSSPDNERITTEEALKLKSDRQKLAASWVQAALSTNLSSFSVYNREPQLSKLPVSTTSNSQNQKIVLGSKPILVLENSREDASLKAHGKTRPAVANSKQALQGTPRKQGDAISNPKKQLVQQPLQEWIKGNDLDAAVNLADMLQLRSRDWFLLFVEKFLDSDGDIGLSNNGQIAGMLTQLKSVNDWLDEIGSSKNEGESCQIPVETIDRLRKKIYEYLLTHVESAAAALTSDSQSSEIKGKK*