Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021158.01.T01 | XP_003606816.1 | 95.873 | 727 | 28 | 1 | 1 | 725 | 1 | 727 | 0 | 1422 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048700.01 | MsG0480021158.01 | 0.807206 | 5.661116e-50 | 3.134198e-47 |
| MsG0180000001.01 | MsG0480021158.01 | 0.802107 | 6.563578e-49 | 3.188121e-46 |
| MsG0180000360.01 | MsG0480021158.01 | 0.844771 | 6.149227e-59 | 1.003258e-55 |
| MsG0180001117.01 | MsG0480021158.01 | 0.814510 | 1.485254e-51 | 9.985945e-49 |
| MsG0180001173.01 | MsG0480021158.01 | 0.819275 | 1.265062e-52 | 9.690632e-50 |
| MsG0180001565.01 | MsG0480021158.01 | 0.800258 | 1.567874e-48 | 7.267228e-46 |
| MsG0180003168.01 | MsG0480021158.01 | 0.816578 | 5.145088e-52 | 3.659703e-49 |
| MsG0180003820.01 | MsG0480021158.01 | 0.813718 | 2.221052e-51 | 1.461562e-48 |
| MsG0180004357.01 | MsG0480021158.01 | 0.850819 | 1.326569e-60 | 2.631491e-57 |
| MsG0180004989.01 | MsG0480021158.01 | 0.808974 | 2.378508e-50 | 1.379274e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021158.01.T01 | MTR_4g068050 | 95.873 | 727 | 28 | 1 | 1 | 725 | 1 | 727 | 0.0 | 1422 |
| MsG0480021158.01.T01 | MTR_2g099095 | 62.681 | 761 | 209 | 17 | 1 | 718 | 1 | 729 | 0.0 | 816 |
| MsG0480021158.01.T01 | MTR_0050s0010 | 63.025 | 119 | 44 | 0 | 1 | 119 | 1 | 119 | 1.15e-47 | 177 |
| MsG0480021158.01.T01 | MTR_0050s0010 | 35.833 | 120 | 60 | 3 | 608 | 712 | 412 | 529 | 3.98e-12 | 69.7 |
| MsG0480021158.01.T01 | MTR_5g034370 | 57.025 | 121 | 51 | 1 | 1 | 120 | 1 | 121 | 2.12e-41 | 160 |
| MsG0480021158.01.T01 | MTR_4g112035 | 56.667 | 120 | 52 | 0 | 1 | 120 | 1 | 120 | 4.67e-40 | 157 |
| MsG0480021158.01.T01 | MTR_2g062300 | 31.680 | 363 | 196 | 13 | 369 | 711 | 254 | 584 | 1.08e-36 | 146 |
| MsG0480021158.01.T01 | MTR_2g062300 | 46.825 | 126 | 61 | 2 | 1 | 124 | 1 | 122 | 1.85e-26 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021158.01.T01 | AT1G08760 | 51.747 | 744 | 298 | 17 | 1 | 706 | 1 | 721 | 0.0 | 676 |
| MsG0480021158.01.T01 | AT3G14170 | 61.345 | 119 | 46 | 0 | 1 | 119 | 1 | 119 | 2.81e-45 | 170 |
| MsG0480021158.01.T01 | AT3G14170 | 35.338 | 133 | 69 | 3 | 594 | 712 | 374 | 503 | 5.12e-13 | 72.8 |
| MsG0480021158.01.T01 | AT3G14170 | 34.831 | 89 | 58 | 0 | 384 | 472 | 250 | 338 | 1.68e-12 | 70.9 |
| MsG0480021158.01.T01 | AT3G14170 | 61.345 | 119 | 46 | 0 | 1 | 119 | 1 | 119 | 2.91e-45 | 169 |
| MsG0480021158.01.T01 | AT3G14170 | 35.338 | 133 | 69 | 3 | 594 | 712 | 375 | 504 | 5.46e-13 | 72.4 |
| MsG0480021158.01.T01 | AT3G14170 | 34.831 | 89 | 58 | 0 | 384 | 472 | 251 | 339 | 1.84e-12 | 70.9 |
| MsG0480021158.01.T01 | AT4G13370 | 58.400 | 125 | 52 | 0 | 1 | 125 | 1 | 125 | 3.01e-42 | 164 |
| MsG0480021158.01.T01 | AT4G13370 | 28.135 | 327 | 160 | 6 | 382 | 707 | 413 | 665 | 2.20e-23 | 105 |
| MsG0480021158.01.T01 | AT1G23790 | 57.377 | 122 | 51 | 1 | 1 | 121 | 1 | 122 | 3.43e-41 | 158 |
Find 151 sgRNAs with CRISPR-Local
Find 241 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGGCAAATCTAGTTCC+TGG | 0.348238 | 4:-54530355 | MsG0480021158.01.T01:CDS |
| AAATCATCCGCGCACTATTC+AGG | 0.311812 | 4:+54527432 | None:intergenic |
| AAATCTATAAAGAAAGTTAA+CGG | 0.457905 | 4:-54528877 | MsG0480021158.01.T01:CDS |
| AAATCTCCACCTGCTAGTGC+TGG | 0.505213 | 4:+54530241 | None:intergenic |
| AAATGGCAAATCTAGTTCCT+GGG | 0.508006 | 4:-54530354 | MsG0480021158.01.T01:CDS |
| AACAATAAATGTGAAATGTG+AGG | 0.588377 | 4:-54526442 | MsG0480021158.01.T01:three_prime_UTR |
| AACACAGACATAAAAGTTGG+TGG | 0.601218 | 4:-54530304 | MsG0480021158.01.T01:CDS |
| AACACCAAGTAAGTCCTCTA+AGG | 0.416936 | 4:-54528917 | MsG0480021158.01.T01:CDS |
| AACAGAAAAGGATGAGAGAT+TGG | 0.439259 | 4:+54527277 | None:intergenic |
| AACGATTGGTTGGATGAGAT+AGG | 0.546451 | 4:-54526831 | MsG0480021158.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACAAAGAAAAGTCCAACA+AGG | + | Chr4:54527620-54527639 | None:intergenic | 30.0% | |
| AACTGATCAATTGTATTCTG+AGG | + | Chr4:54529261-54529280 | None:intergenic | 30.0% | |
| AAGCAACAAGGAAATATCAA+TGG | - | Chr4:54527033-54527052 | MsG0480021158.01.T01:CDS | 30.0% | |
| AAGCTGAAACTGCAAAAATA+AGG | + | Chr4:54528078-54528097 | None:intergenic | 30.0% | |
| AATTCAAACTCTGTCTCTTT+AGG | + | Chr4:54529027-54529046 | None:intergenic | 30.0% | |
| ACTGATCAATTGTATTCTGA+GGG | + | Chr4:54529260-54529279 | None:intergenic | 30.0% | |
| ATAAAGAAAGTTAACGGTGA+TGG | - | Chr4:54527860-54527879 | MsG0480021158.01.T01:intron | 30.0% | |
| ATAAAGAGACATCCAAATCA+AGG | - | Chr4:54527235-54527254 | MsG0480021158.01.T01:CDS | 30.0% | |
| ATAGGATCGAGTAAAAATGA+GGG | - | Chr4:54529918-54529937 | MsG0480021158.01.T01:CDS | 30.0% | |
| ATGAACACAGACATAAAAGT+TGG | - | Chr4:54526424-54526443 | MsG0480021158.01.T01:three_prime_UTR | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 54526365 | 54530388 | 54526365 | ID=MsG0480021158.01;Name=MsG0480021158.01 |
| Chr4 | mRNA | 54526365 | 54530388 | 54526365 | ID=MsG0480021158.01.T01;Parent=MsG0480021158.01;Name=MsG0480021158.01.T01;_AED=0.24;_eAED=0.24;_QI=14|1|1|1|1|1|4|309|725 |
| Chr4 | exon | 54526365 | 54527535 | 54526365 | ID=MsG0480021158.01.T01:exon:8917;Parent=MsG0480021158.01.T01 |
| Chr4 | exon | 54528118 | 54528209 | 54528118 | ID=MsG0480021158.01.T01:exon:8916;Parent=MsG0480021158.01.T01 |
| Chr4 | exon | 54528699 | 54529001 | 54528699 | ID=MsG0480021158.01.T01:exon:8915;Parent=MsG0480021158.01.T01 |
| Chr4 | exon | 54529454 | 54530388 | 54529454 | ID=MsG0480021158.01.T01:exon:8914;Parent=MsG0480021158.01.T01 |
| Chr4 | five_prime_UTR | 54530375 | 54530388 | 54530375 | ID=MsG0480021158.01.T01:five_prime_utr;Parent=MsG0480021158.01.T01 |
| Chr4 | CDS | 54529454 | 54530374 | 54529454 | ID=MsG0480021158.01.T01:cds;Parent=MsG0480021158.01.T01 |
| Chr4 | CDS | 54528699 | 54529001 | 54528699 | ID=MsG0480021158.01.T01:cds;Parent=MsG0480021158.01.T01 |
| Chr4 | CDS | 54528118 | 54528209 | 54528118 | ID=MsG0480021158.01.T01:cds;Parent=MsG0480021158.01.T01 |
| Chr4 | CDS | 54526674 | 54527535 | 54526674 | ID=MsG0480021158.01.T01:cds;Parent=MsG0480021158.01.T01 |
| Chr4 | three_prime_UTR | 54526365 | 54526673 | 54526365 | ID=MsG0480021158.01.T01:three_prime_utr;Parent=MsG0480021158.01.T01 |
| Gene Sequence |
>MsG0480021158.01.T01 ATGGCAAATCTAGTTCCTGGGGTGCTACTAAAGTTAATGCAGCACATGAACACAGACATAAAAGTTGGTGGTGAACATAGATCATCATTGTTACAAGTTGTGAGCATTGTTCCAGCACTAGCAGGTGGAGATTTGTTTACAAATCAAGGCTTTTATGTTAAGGTTTCTGATTCATCACATGCTACTTATGTCTCTCTCCCTGATGAACATGATGATCTAATCCTTAGTGACAAGATTCAGTTAGGCCAGTTTGTTTTTGTTGATCGGTTTGAAGCTGCTTCGCCTGTTCCGATTCTTCGTGGTGTTAGGCCTGTTCCTGGGAGACATGCTTGTGTTGGAACACCTGAAGATATTGTTGCAACTAACTCACTTGGTTTTCTTAGCAATGCTTCTGAGGTTAAGGTTAAGAGGAATAGTGTTTGTTCCAAGTCCTCTCCAATGAAAGTGTTGGAGAATCATCAAGAACAAGTAGATAAGAAATCAATGGTTTTAGGTAGATGTAAATCTCAGACAGCAAAAGATTCTGTTGTTGATTTTGTGAAGAAGGAAAAGTTAACAAGATTAAAGTCATTGAATTCAAGAGCTATTGTTCCTTCTTCACCTACTAGTTGCTATTCTTTGCCTACTTCTTTTGAGAAATTCTCTAATGGAGTTAAGCAACAAGGAAATATCAATGGAGTGGATAGGCTGATTGCTAAGGTGGGGGTGGTGGAGACGGGAAAGGGTGTTCGCGGAGTTAGTCCTCTCGGGAAGAGAATTGCAGTTGGAAATTCAATCAGAAATTTAGTTCAAGGAATTGAACTTGGTGCTAAGGCGTTGCGGAAAAGCTGGGAAGGGAACATGGAAGTTAAGAATAATAAAGAGACATCCAAATCAAGGGCATCTTCTAAGTGTGATTCTAAGCCTGAATTTCGTAGCACAACTCCTAGGAAAAGTACATCGAGTGAGAAGTTTCCTTCTAAAGATTACGAGAGTAAGACACAAACACCAAGTAAGTCCTCTAAGGAAGAAAACAAGATTCAAAAATCTATAAAGAAAGTTAACGGTGATGGAACTATGGAAGAACAAGAAAAGACAACGAAGCAAAGGAACTCTTTTGGAAAGAAATCTTCAGAAGCCGGACTTCCCGGAAACTTTGTCAAGGTTTCAGTAAATAGTAAAAAAGTGACAGAAGCAAGTGTTCAATGGACTTCACTCCCTTCATCTATTGCGAAGCTTGGGAGGGAAGTAATGAAGCACAGAGATGCAGCACAGATGGCAGCAACTGAGGCTATGCAAGAGGCTGCTACTGCTGATAGTTTGCTACAATGTCTTAGTGTGTATGCAGAGCTAAGTAATTCTGCCAAGGAACATAACCCTCAGAATACAATTGATCAGTTTTTAACGCTTCACACTAGCCTGAATAGTGCGCGGATGATTTCAGATTCCCTTTCTAAAACCATTCCAGATGGTTCATCTCCAGATAATGAAAGAATCACAACAGAAGAAGCACTAAAACTCAAATCAGATAGACAAAAACTTGCAGCTTCTTGGGTCCAAGCTGCCTTATCCACCAATCTCTCATCCTTTTCTGTTTATAACCGAGAGCCTCAATTGTCCAAGCTTCCTGTTTCAACTACAAGTAATTCTCAAAATCAAAAGATTGTTCTTGGAAGTAAACCAATTTTAGTCCTCGAAAATTCAAGAGAAGACGCTTCATTGAAAGCTCATGGAAAAACTCGTCCAGCAGTAGCTAATTCCAAACAAGCCTTACAAGGAACTCCGCGCAAACAAGGTGATGCAATATCAAATCCAAAAAAGCAACTAGTCCAACAACCACTCCAAGAGTGGATTAAAGGAAATGACCTTGATGCGGCGGTTAATTTAGCCGACATGCTGCAATTGCGATCGCGAGACTGGTTTTTATTGTTTGTTGAGAAATTTTTGGACAGTGATGGAGACATTGGTTTGTCAAACAATGGTCAAATAGCAGGCATGCTCACTCAACTTAAAAGCGTCAACGATTGGTTGGATGAGATAGGATCGAGTAAAAATGAGGGGGAATCATGTCAGATACCGGTAGAGACAATTGATAGACTCAGGAAGAAGATATACGAATATCTTCTTACACATGTTGAATCTGCTGCTGCTGCCCTTACTTCTGACTCACAATCATCAGAGATCAAAGGCAAAAAGTGA |
| Protein sequence |
>MsG0480021158.01.T01 MANLVPGVLLKLMQHMNTDIKVGGEHRSSLLQVVSIVPALAGGDLFTNQGFYVKVSDSSHATYVSLPDEHDDLILSDKIQLGQFVFVDRFEAASPVPILRGVRPVPGRHACVGTPEDIVATNSLGFLSNASEVKVKRNSVCSKSSPMKVLENHQEQVDKKSMVLGRCKSQTAKDSVVDFVKKEKLTRLKSLNSRAIVPSSPTSCYSLPTSFEKFSNGVKQQGNINGVDRLIAKVGVVETGKGVRGVSPLGKRIAVGNSIRNLVQGIELGAKALRKSWEGNMEVKNNKETSKSRASSKCDSKPEFRSTTPRKSTSSEKFPSKDYESKTQTPSKSSKEENKIQKSIKKVNGDGTMEEQEKTTKQRNSFGKKSSEAGLPGNFVKVSVNSKKVTEASVQWTSLPSSIAKLGREVMKHRDAAQMAATEAMQEAATADSLLQCLSVYAELSNSAKEHNPQNTIDQFLTLHTSLNSARMISDSLSKTIPDGSSPDNERITTEEALKLKSDRQKLAASWVQAALSTNLSSFSVYNREPQLSKLPVSTTSNSQNQKIVLGSKPILVLENSREDASLKAHGKTRPAVANSKQALQGTPRKQGDAISNPKKQLVQQPLQEWIKGNDLDAAVNLADMLQLRSRDWFLLFVEKFLDSDGDIGLSNNGQIAGMLTQLKSVNDWLDEIGSSKNEGESCQIPVETIDRLRKKIYEYLLTHVESAAAALTSDSQSSEIKGKK* |
