AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021246.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021246.01.T01 MTR_4g070020 93.814 97 5 1 1 96 171 267 1.43e-61 189
MsG0480021246.01.T01 MTR_4g070020 95.455 66 2 1 1 65 171 236 1.11e-37 127
MsG0480021246.01.T01 MTR_1g022190 39.175 97 52 4 7 96 172 268 1.01e-13 65.1
MsG0480021246.01.T01 MTR_1g022190 39.175 97 52 4 7 96 197 293 1.12e-13 65.1
MsG0480021246.01.T01 MTR_1g022190 39.175 97 52 4 7 96 202 298 1.43e-13 64.7
MsG0480021246.01.T01 MTR_1g022190 51.923 52 24 1 7 57 197 248 4.94e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021246.01.T01 AT1G75180 51.648 91 42 2 6 94 179 269 7.55e-20 82.0
MsG0480021246.01.T01 AT1G75180 51.648 91 42 2 6 94 179 269 7.55e-20 82.0
MsG0480021246.01.T01 AT1G19400 48.936 94 46 2 6 98 172 264 1.41e-17 75.5
MsG0480021246.01.T01 AT1G19400 48.936 94 46 2 6 98 172 264 1.41e-17 75.5
MsG0480021246.01.T01 AT1G19400 48.936 94 46 2 6 98 172 264 1.41e-17 75.5
MsG0480021246.01.T01 AT1G19400 59.677 62 24 1 6 66 172 233 1.87e-15 69.3
MsG0480021246.01.T01 AT1G75180 60.714 56 22 0 6 61 179 234 2.31e-15 68.9
MsG0480021246.01.T01 AT1G75180 60.714 56 22 0 6 61 179 234 2.53e-15 69.3

Find 120 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAGGCACAAAGTTCTTTA+AGG 0.155540 4:-55776833 MsG0480021246.01.T01:five_prime_UTR
AGGTGGTAATAAATGATTTA+AGG 0.188726 4:-55775431 MsG0480021246.01.T01:three_prime_UTR
CATGGTTAACGCCGGTTGTT+AGG 0.191299 4:-55775812 MsG0480021246.01.T01:exon
ATGGATATTGTTTAATTTAA+TGG 0.209398 4:+55778254 None:intergenic
GATACCCTTCTTGAAGTTAA+TGG 0.212761 4:-55777105 MsG0480021246.01.T01:five_prime_UTR
GAGAATGATGCAAATGAAAA+TGG 0.217056 4:-55776659 MsG0480021246.01.T01:CDS
TCCTTCTGCGCTTGCTTTGC+AGG 0.233001 4:-55776630 MsG0480021246.01.T01:intron
CTTTAAGCTTCAATAGATTC+TGG 0.234279 4:-55778154 MsG0480021246.01.T01:five_prime_UTR
AGACATTAACATGTTGTCTT+TGG 0.250262 4:+55775596 None:intergenic
GTTAGGGTTGGTGTTGGTAT+TGG 0.255304 4:-55775795 MsG0480021246.01.T01:three_prime_UTR
AAGAACAAATCTTGCAATCT+TGG 0.266859 4:+55778174 None:intergenic
AAGATATATATTATATAGTT+TGG 0.276441 4:-55775557 MsG0480021246.01.T01:three_prime_UTR
GAGAATTCGTATAAGGTTAA+TGG 0.283457 4:-55777305 MsG0480021246.01.T01:five_prime_UTR
GGAAATGGACCAAGTGAAAA+TGG 0.283915 4:-55777284 MsG0480021246.01.T01:five_prime_UTR
AAGAAGGGTATCAGGATTAA+AGG 0.303777 4:+55777116 None:intergenic
ATTGCTGTTACATAATTCTA+AGG 0.316159 4:+55776920 None:intergenic
CTCATATCAACCCTCGATAA+AGG 0.321091 4:-55777237 MsG0480021246.01.T01:five_prime_UTR
GACATTAACATGTTGTCTTT+GGG 0.346532 4:+55775597 None:intergenic
AAAGATAAGCTTCTTCTGTT+TGG 0.350138 4:-55776945 MsG0480021246.01.T01:five_prime_UTR
TTAACTTCAAGAAGGGTATC+AGG 0.353127 4:+55777108 None:intergenic
ATCAAAACATAGGTTGTTAT+CGG 0.360179 4:+55778202 None:intergenic
TCGTATAAGGTTAATGGAAA+TGG 0.363071 4:-55777299 MsG0480021246.01.T01:five_prime_UTR
GGCGAAGAGGGGAACTTTCA+TGG 0.371286 4:-55775830 MsG0480021246.01.T01:CDS
AGAATAGCCGTCTTCTGAAT+TGG 0.372025 4:+55775704 None:intergenic
AGGAGAAGCAATGTTGTTGC+CGG 0.374625 4:-55776813 MsG0480021246.01.T01:exon
AGAACAAATCTTGCAATCTT+GGG 0.374948 4:+55778175 None:intergenic
TATATTGCCGGGTCCTTCTC+TGG 0.379377 4:-55776764 MsG0480021246.01.T01:CDS
CGTTACCGTGGTTAGACATA+AGG 0.381604 4:-55777211 MsG0480021246.01.T01:five_prime_UTR
ACAATTGAGGTCTATATTGC+CGG 0.393367 4:-55776776 MsG0480021246.01.T01:CDS
TTGTAAGCACTGATAGTATA+AGG 0.394406 4:-55777000 MsG0480021246.01.T01:five_prime_UTR
TTTAAGCTTCAATAGATTCT+GGG 0.398434 4:-55778153 MsG0480021246.01.T01:five_prime_UTR
TGTTTGTCATACATGTAATC+TGG 0.402010 4:+55775902 None:intergenic
GAAAACTGAAATGTTATGTT+GGG 0.402948 4:+55778407 None:intergenic
GGTGGTAATAAATGATTTAA+GGG 0.404373 4:-55775430 MsG0480021246.01.T01:three_prime_UTR
CGCGATGGAGAATTCGTATA+AGG 0.406902 4:-55777312 MsG0480021246.01.T01:five_prime_UTR
TTAGTCGGCCATCTTTATCT+TGG 0.415591 4:-55775620 MsG0480021246.01.T01:three_prime_UTR
TTTGTGTGTGAATTAATTAG+AGG 0.422085 4:+55778349 None:intergenic
TTCTTGAAGTTAATGGTGTT+CGG 0.422504 4:-55777098 MsG0480021246.01.T01:five_prime_UTR
TGTTAGAGAATCAATTCTAA+TGG 0.424493 4:-55776892 MsG0480021246.01.T01:five_prime_UTR
TGATTAAGTGTGAGAATCTC+AGG 0.428692 4:+55777143 None:intergenic
CGGAGTAGTTTATATTCTGA+TGG 0.435949 4:-55777078 MsG0480021246.01.T01:five_prime_UTR
GGGATTCCAACCTTCTCAAA+AGG 0.436700 4:-55777057 MsG0480021246.01.T01:five_prime_UTR
GCAATATAGACCTCAATTGT+TGG 0.437528 4:+55776779 None:intergenic
TTATATTCCCCTACTTATTG+AGG 0.438498 4:-55775451 MsG0480021246.01.T01:three_prime_UTR
CTTGGAATCGGCATTGGAGT+TGG 0.441010 4:-55775760 MsG0480021246.01.T01:three_prime_UTR
TTATTACCACCTCAATAAGT+AGG 0.441077 4:+55775442 None:intergenic
TCCTTAAAGAACTTTGTGCC+TGG 0.447833 4:+55776832 None:intergenic
GGATAGGGTGGATAGAAAAT+CGG 0.450168 4:-55777035 MsG0480021246.01.T01:five_prime_UTR
GTTTGTCTTGGAATCGGCAT+TGG 0.451016 4:-55775766 MsG0480021246.01.T01:three_prime_UTR
TGTGAGTCGAATATAATTCC+AGG 0.452189 4:-55776850 MsG0480021246.01.T01:five_prime_UTR
ACTAGTGTTTGTCTTGGAAT+CGG 0.452588 4:-55775772 MsG0480021246.01.T01:three_prime_UTR
TGAAAACTGAAATGTTATGT+TGG 0.452656 4:+55778406 None:intergenic
ATGGTTAACGCCGGTTGTTA+GGG 0.456320 4:-55775811 MsG0480021246.01.T01:exon
AAGAATAATATCAGATTCAA+AGG 0.458806 4:-55778289 MsG0480021246.01.T01:five_prime_UTR
GAATTAATTAGAGGAAGAAA+AGG 0.460023 4:+55778358 None:intergenic
TCATATCAACCCTCGATAAA+GGG 0.470195 4:-55777236 MsG0480021246.01.T01:five_prime_UTR
AGAATGATGCAAATGAAAAT+GGG 0.470852 4:-55776658 MsG0480021246.01.T01:CDS
CGTATGCTGTTCTTGTGTAT+AGG 0.473133 4:+55775858 None:intergenic
TGAATTGGCCGGTGGCACCT+TGG 0.475731 4:+55775719 None:intergenic
TCAATTCTAATGGACAAAGT+TGG 0.484626 4:-55776882 MsG0480021246.01.T01:five_prime_UTR
TATTACCACCTCAATAAGTA+GGG 0.486522 4:+55775443 None:intergenic
AAGATAAAGATGGCCGACTA+AGG 0.490642 4:+55775622 None:intergenic
CCAACACCAACCCTAACAAC+CGG 0.490828 4:+55775801 None:intergenic
CCAACCTTCTCAAAAGGGAT+AGG 0.492483 4:-55777051 MsG0480021246.01.T01:five_prime_UTR
GTTTAATTTAATGGAAGATG+TGG 0.493650 4:+55778263 None:intergenic
AAGGAGAGTGTGAAAATTGT+TGG 0.497096 4:+55778377 None:intergenic
TATTGGACTAGTGTTTGTCT+TGG 0.497559 4:-55775778 MsG0480021246.01.T01:three_prime_UTR
GTTACCGTGGTTAGACATAA+GGG 0.505953 4:-55777210 MsG0480021246.01.T01:five_prime_UTR
TTAACGCCGGTTGTTAGGGT+TGG 0.509353 4:-55775807 MsG0480021246.01.T01:exon
CCGGTTGTTAGGGTTGGTGT+TGG 0.512038 4:-55775801 MsG0480021246.01.T01:three_prime_UTR
GGAGTAGTTTATATTCTGAT+GGG 0.528712 4:-55777077 MsG0480021246.01.T01:five_prime_UTR
GGATTCCAACCTTCTCAAAA+GGG 0.534401 4:-55777056 MsG0480021246.01.T01:five_prime_UTR
ACCTGCAAAGCAAGCGCAGA+AGG 0.534939 4:+55776629 None:intergenic
AGAATAATATCAGATTCAAA+GGG 0.538270 4:-55778288 MsG0480021246.01.T01:five_prime_UTR
GGTTGTTATCGGTTATGTTG+AGG 0.539393 4:+55778213 None:intergenic
CAACCTTCTCAAAAGGGATA+GGG 0.543798 4:-55777050 MsG0480021246.01.T01:five_prime_UTR
GAGTGGTAGTGTGAAGTTTG+AGG 0.549063 4:-55776975 MsG0480021246.01.T01:five_prime_UTR
CTTGGGATGAATCAAAACAT+AGG 0.551006 4:+55778192 None:intergenic
CTCTACTCTTCCAACAATTG+AGG 0.556408 4:-55776789 MsG0480021246.01.T01:CDS
TATTCCCCTACTTATTGAGG+TGG 0.561142 4:-55775448 MsG0480021246.01.T01:three_prime_UTR
TGAGGTGGTAAGAGAAGATG+TGG 0.564391 4:+55778231 None:intergenic
GTGGTAATAAATGATTTAAG+GGG 0.565067 4:-55775429 MsG0480021246.01.T01:three_prime_UTR
AAATCATACCAAGGTGCCAC+CGG 0.568352 4:-55775727 MsG0480021246.01.T01:three_prime_UTR
GAACACCATTAACTTCAAGA+AGG 0.569037 4:+55777100 None:intergenic
GCATACGCTGACGGCGAAGA+GGG 0.569062 4:-55775842 MsG0480021246.01.T01:CDS
TGTTATCGGTTATGTTGAGG+TGG 0.575774 4:+55778216 None:intergenic
TTTAATTTAATGGAAGATGT+GGG 0.578479 4:+55778264 None:intergenic
TAGCCGTCTTCTGAATTGGC+CGG 0.581948 4:+55775708 None:intergenic
CAATTGAGGTCTATATTGCC+GGG 0.582339 4:-55776775 MsG0480021246.01.T01:CDS
CCACCGGCCAATTCAGAAGA+CGG 0.588469 4:-55775711 MsG0480021246.01.T01:three_prime_UTR
TCTGGAATTGCATTCAACGC+CGG 0.588676 4:+55776680 None:intergenic
CCTTCTCAAAAGGGATAGGG+TGG 0.588969 4:-55777047 MsG0480021246.01.T01:five_prime_UTR
AAAATGGATACTCTATTGCT+AGG 0.589815 4:-55777268 MsG0480021246.01.T01:five_prime_UTR
CGATAAAGGGTTCGTTACCG+TGG 0.592561 4:-55777223 MsG0480021246.01.T01:five_prime_UTR
AAGGTCTTCGTTAAGATGAT+CGG 0.592566 4:+55776737 None:intergenic
CGGAGTGCCAGAGAAGGACC+CGG 0.592882 4:+55776757 None:intergenic
GATGCAAATGAAAATGGGAA+AGG 0.596141 4:-55776653 MsG0480021246.01.T01:CDS
AACACCATTAACTTCAAGAA+GGG 0.596194 4:+55777101 None:intergenic
ATTGTTGGAAGAGTAGAGTC+CGG 0.603903 4:+55776794 None:intergenic
TGGTAATAAATGATTTAAGG+GGG 0.603940 4:-55775428 MsG0480021246.01.T01:three_prime_UTR
GTGGTAAGAGAAGATGTGGA+TGG 0.604928 4:+55778235 None:intergenic
ACAAGAACAGCATACGCTGA+CGG 0.606360 4:-55775851 MsG0480021246.01.T01:CDS
TCTTTGGGCCAAGATAAAGA+TGG 0.610464 4:+55775612 None:intergenic
AAAGTAGAACTGTCTCGCGA+TGG 0.611108 4:-55777327 MsG0480021246.01.T01:five_prime_UTR
AAAACTGAAATGTTATGTTG+GGG 0.627111 4:+55778408 None:intergenic
AGCATACGCTGACGGCGAAG+AGG 0.627567 4:-55775843 MsG0480021246.01.T01:CDS
CGGTAACGAACCCTTTATCG+AGG 0.628590 4:+55777226 None:intergenic
ACTGATAGTATAAGGATGAG+TGG 0.634943 4:-55776992 MsG0480021246.01.T01:five_prime_UTR
CATGATGATGAGGATTATCA+CGG 0.635768 4:-55775881 MsG0480021246.01.T01:CDS
GATGATCGGAGTGCCAGAGA+AGG 0.637274 4:+55776751 None:intergenic
CCGTCTTCTGAATTGGCCGG+TGG 0.642624 4:+55775711 None:intergenic
GGTAACGAACCCTTTATCGA+GGG 0.642976 4:+55777227 None:intergenic
GGAACTTTCATGGTTAACGC+CGG 0.646841 4:-55775820 MsG0480021246.01.T01:exon
AACACCCTTATGTCTAACCA+CGG 0.647619 4:+55777206 None:intergenic
TTAGAATTATGTAACAGCAA+TGG 0.648523 4:-55776918 MsG0480021246.01.T01:five_prime_UTR
ATTACCACCTCAATAAGTAG+GGG 0.652127 4:+55775444 None:intergenic
CATACGCTGACGGCGAAGAG+GGG 0.659414 4:-55775841 MsG0480021246.01.T01:CDS
AAGTGTGAGAATCTCAGGAG+TGG 0.662685 4:+55777148 None:intergenic
GTATGACAAACATGATGATG+AGG 0.669080 4:-55775891 MsG0480021246.01.T01:CDS
ATACTCGTTAAATCATACCA+AGG 0.711189 4:-55775736 MsG0480021246.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATATATATTATATAGTT+TGG - Chr4:55778300-55778319 MsG0480021246.01.T01:five_prime_UTR 10.0%
!!! CAAAATTTGATTTTTAATTT+TGG - Chr4:55775785-55775804 MsG0480021246.01.T01:three_prime_UTR 10.0%
!! AATTACATAAGAAAATCAAA+AGG + Chr4:55776437-55776456 None:intergenic 15.0%
!! CAAAGAAATATAAGAATTAA+AGG + Chr4:55776175-55776194 None:intergenic 15.0%
!!! AAATTTTGATTATTGTTATG+TGG + Chr4:55775773-55775792 None:intergenic 15.0%
!!! AAATTTTTAAAATCTTGTCA+AGG + Chr4:55777840-55777859 None:intergenic 15.0%
!!! ATGGATATTGTTTAATTTAA+TGG + Chr4:55775606-55775625 None:intergenic 15.0%
!! AACATAACATATCAACTATT+CGG + Chr4:55776052-55776071 None:intergenic 20.0%
!! AAGAATAATATCAGATTCAA+AGG - Chr4:55775568-55775587 MsG0480021246.01.T01:three_prime_UTR 20.0%
!! AGAATAATATCAGATTCAAA+GGG - Chr4:55775569-55775588 MsG0480021246.01.T01:three_prime_UTR 20.0%
!! ATTTAAATAAGCACATGAAA+AGG + Chr4:55777276-55777295 None:intergenic 20.0%
!! CAATCAGATAAAAATAATAC+AGG + Chr4:55776244-55776263 None:intergenic 20.0%
!! CAGCATTTACATAAATAATA+AGG + Chr4:55777929-55777948 None:intergenic 20.0%
!! GATATAACTGTAAATTATCT+TGG - Chr4:55777670-55777689 MsG0480021246.01.T01:intron 20.0%
!!! AGGAATTATCTATAATTTGT+TGG + Chr4:55777909-55777928 None:intergenic 20.0%
!!! ATTTATTTGTGCTTTTATGT+TGG - Chr4:55776369-55776388 MsG0480021246.01.T01:intron 20.0%
!!! GGAATTATCTATAATTTGTT+GGG + Chr4:55777908-55777927 None:intergenic 20.0%
!!! TATTTTTATCTGATTGAGTA+AGG - Chr4:55776248-55776267 MsG0480021246.01.T01:intron 20.0%
!!! TTGAATTTTGCATTAGTTAA+TGG - Chr4:55775853-55775872 MsG0480021246.01.T01:CDS 20.0%
!!! TTTAATTTAATGGAAGATGT+GGG + Chr4:55775596-55775615 None:intergenic 20.0%
! AAAACTGAAATGTTATGTTG+GGG + Chr4:55775452-55775471 None:intergenic 25.0%
! AGAAAATCAAAAGGTTGAAA+AGG + Chr4:55776428-55776447 None:intergenic 25.0%
! AGAATGATGCAAATGAAAAT+GGG - Chr4:55777199-55777218 MsG0480021246.01.T01:five_prime_UTR 25.0%
! AGGTGGTAATAAATGATTTA+AGG - Chr4:55778426-55778445 MsG0480021246.01.T01:five_prime_UTR 25.0%
! ATATTTATGACCAAACCAAA+TGG - Chr4:55777860-55777879 MsG0480021246.01.T01:intron 25.0%
! ATCAAAACATAGGTTGTTAT+CGG + Chr4:55775658-55775677 None:intergenic 25.0%
! ATTATTTATGTAAATGCTGC+AGG - Chr4:55777930-55777949 MsG0480021246.01.T01:intron 25.0%
! ATTCTCATAAGATTTCCATT+TGG + Chr4:55777878-55777897 None:intergenic 25.0%
! ATTTAGACAAAAAAAACAGC+CGG + Chr4:55775940-55775959 None:intergenic 25.0%
! GAAAACTGAAATGTTATGTT+GGG + Chr4:55775453-55775472 None:intergenic 25.0%
! GAATTAATTAGAGGAAGAAA+AGG + Chr4:55775502-55775521 None:intergenic 25.0%
! GGTGGTAATAAATGATTTAA+GGG - Chr4:55778427-55778446 MsG0480021246.01.T01:five_prime_UTR 25.0%
! GTGGTAATAAATGATTTAAG+GGG - Chr4:55778428-55778447 MsG0480021246.01.T01:five_prime_UTR 25.0%
! TGAAAACTGAAATGTTATGT+TGG + Chr4:55775454-55775473 None:intergenic 25.0%
! TGGTAATAAATGATTTAAGG+GGG - Chr4:55778429-55778448 MsG0480021246.01.T01:five_prime_UTR 25.0%
! TGTTAGAGAATCAATTCTAA+TGG - Chr4:55776965-55776984 MsG0480021246.01.T01:five_prime_UTR 25.0%
! TTAGAATTATGTAACAGCAA+TGG - Chr4:55776939-55776958 MsG0480021246.01.T01:five_prime_UTR 25.0%
! TTTAAGCTTCAATAGATTCT+GGG - Chr4:55775704-55775723 MsG0480021246.01.T01:three_prime_UTR 25.0%
! TTTGTGTGTGAATTAATTAG+AGG + Chr4:55775511-55775530 None:intergenic 25.0%
!! ATTGCTGTTACATAATTCTA+AGG + Chr4:55776940-55776959 None:intergenic 25.0%
!! GAATAGTTGATATGTTATGT+TGG - Chr4:55776051-55776070 MsG0480021246.01.T01:intron 25.0%
!! GTTTAATTTAATGGAAGATG+TGG + Chr4:55775597-55775616 None:intergenic 25.0%
!! TACAAAGTGTTCTTAAATAG+TGG - Chr4:55777445-55777464 MsG0480021246.01.T01:intron 25.0%
!!! TCTATAATTTGTTGGGAAAA+TGG + Chr4:55777901-55777920 None:intergenic 25.0%
AAAATGGATACTCTATTGCT+AGG - Chr4:55776589-55776608 MsG0480021246.01.T01:intron 30.0%
AAAGATAAGCTTCTTCTGTT+TGG - Chr4:55776912-55776931 MsG0480021246.01.T01:five_prime_UTR 30.0%
AACACCATTAACTTCAAGAA+GGG + Chr4:55776759-55776778 None:intergenic 30.0%
AAGAACAAATCTTGCAATCT+TGG + Chr4:55775686-55775705 None:intergenic 30.0%
AGAACAAATCTTGCAATCTT+GGG + Chr4:55775685-55775704 None:intergenic 30.0%
AGACATTAACATGTTGTCTT+TGG + Chr4:55778264-55778283 None:intergenic 30.0%
ATAACATATCAACTATTCGG+TGG + Chr4:55776049-55776068 None:intergenic 30.0%
ATACTCGTTAAATCATACCA+AGG - Chr4:55778121-55778140 MsG0480021246.01.T01:five_prime_UTR 30.0%
ATTCAAGCAACAAGTTAACA+AGG + Chr4:55777328-55777347 None:intergenic 30.0%
CATAAGATTTCCATTTGGTT+TGG + Chr4:55777873-55777892 None:intergenic 30.0%
CTTTAAGCTTCAATAGATTC+TGG - Chr4:55775703-55775722 MsG0480021246.01.T01:three_prime_UTR 30.0%
GACATTAACATGTTGTCTTT+GGG + Chr4:55778263-55778282 None:intergenic 30.0%
GAGAATGATGCAAATGAAAA+TGG - Chr4:55777198-55777217 MsG0480021246.01.T01:five_prime_UTR 30.0%
GAGAATTCGTATAAGGTTAA+TGG - Chr4:55776552-55776571 MsG0480021246.01.T01:intron 30.0%
GGAGTAGTTTATATTCTGAT+GGG - Chr4:55776780-55776799 MsG0480021246.01.T01:CDS 30.0%
GTTCTCAAAAAAGACATACA+AGG - Chr4:55777151-55777170 MsG0480021246.01.T01:five_prime_UTR 30.0%
TATTACCACCTCAATAAGTA+GGG + Chr4:55778417-55778436 None:intergenic 30.0%
TCGTATAAGGTTAATGGAAA+TGG - Chr4:55776558-55776577 MsG0480021246.01.T01:intron 30.0%
TGTTTGTCATACATGTAATC+TGG + Chr4:55777958-55777977 None:intergenic 30.0%
TTATATTCCCCTACTTATTG+AGG - Chr4:55778406-55778425 MsG0480021246.01.T01:five_prime_UTR 30.0%
TTATTACCACCTCAATAAGT+AGG + Chr4:55778418-55778437 None:intergenic 30.0%
TTCTTGAAGTTAATGGTGTT+CGG - Chr4:55776759-55776778 MsG0480021246.01.T01:CDS 30.0%
! GATTTGTTCAAATCCCTAAA+GGG + Chr4:55777398-55777417 None:intergenic 30.0%
! TAGTTGATATGTTATGTTGG+AGG - Chr4:55776054-55776073 MsG0480021246.01.T01:intron 30.0%
! TATTTGTGCTTTTATGTTGG+TGG - Chr4:55776372-55776391 MsG0480021246.01.T01:intron 30.0%
! TCAATTCTAATGGACAAAGT+TGG - Chr4:55776975-55776994 MsG0480021246.01.T01:five_prime_UTR 30.0%
! TTTTCATTTGCATCATTCTC+TGG + Chr4:55777198-55777217 None:intergenic 30.0%
! TTTTGGCACAAGATATTTTG+TGG - Chr4:55775802-55775821 MsG0480021246.01.T01:three_prime_UTR 30.0%
!! ATAGAGTATCCATTTTCACT+TGG + Chr4:55776585-55776604 None:intergenic 30.0%
!! ATTGGCTCAACGTAATTTTA+TGG - Chr4:55777555-55777574 MsG0480021246.01.T01:intron 30.0%
!! TTGTAAGCACTGATAGTATA+AGG - Chr4:55776857-55776876 MsG0480021246.01.T01:five_prime_UTR 30.0%
!! TTTTTGAGAACTAAGATGCA+AGG + Chr4:55777142-55777161 None:intergenic 30.0%
!!! ACATAAGGGTGTTTTATGTA+AGG - Chr4:55776661-55776680 MsG0480021246.01.T01:CDS 30.0%
!!! CATAAGGGTGTTTTATGTAA+GGG - Chr4:55776662-55776681 MsG0480021246.01.T01:CDS 30.0%
!!! GTTTTCTGTTTGCTGTATAA+GGG + Chr4:55775430-55775449 None:intergenic 30.0%
!!! TTTTCTGTTTGCTGTATAAG+GGG + Chr4:55775429-55775448 None:intergenic 30.0%
AAAAAGACATACAAGGAAGC+CGG - Chr4:55777158-55777177 MsG0480021246.01.T01:five_prime_UTR 35.0%
AAGGTCTTCGTTAAGATGAT+CGG + Chr4:55777123-55777142 None:intergenic 35.0%
ACAATTGAGGTCTATATTGC+CGG - Chr4:55777081-55777100 MsG0480021246.01.T01:five_prime_UTR 35.0%
ACTAGTGTTTGTCTTGGAAT+CGG - Chr4:55778085-55778104 MsG0480021246.01.T01:five_prime_UTR 35.0%
ACTGATAGTATAAGGATGAG+TGG - Chr4:55776865-55776884 MsG0480021246.01.T01:five_prime_UTR 35.0%
ATAAATGATTTAAGGGGGCT+TGG - Chr4:55778434-55778453 MsG0480021246.01.T01:five_prime_UTR 35.0%
ATTACCACCTCAATAAGTAG+GGG + Chr4:55778416-55778435 None:intergenic 35.0%
ATTATGTTCCATCTCCTACT+AGG + Chr4:55777642-55777661 None:intergenic 35.0%
CAAAAACAGCTAACCTTAGT+CGG - Chr4:55778222-55778241 MsG0480021246.01.T01:five_prime_UTR 35.0%
CCTGAAAAACAGACAAAAAG+AGG + Chr4:55776100-55776119 None:intergenic 35.0%
CGGAGTAGTTTATATTCTGA+TGG - Chr4:55776779-55776798 MsG0480021246.01.T01:CDS 35.0%
CTTGGGATGAATCAAAACAT+AGG + Chr4:55775668-55775687 None:intergenic 35.0%
GAACACCATTAACTTCAAGA+AGG + Chr4:55776760-55776779 None:intergenic 35.0%
GATACCCTTCTTGAAGTTAA+TGG - Chr4:55776752-55776771 MsG0480021246.01.T01:CDS 35.0%
GATGCAAATGAAAATGGGAA+AGG - Chr4:55777204-55777223 MsG0480021246.01.T01:five_prime_UTR 35.0%
GCAATATAGACCTCAATTGT+TGG + Chr4:55777081-55777100 None:intergenic 35.0%
TCATATCAACCCTCGATAAA+GGG - Chr4:55776621-55776640 MsG0480021246.01.T01:intron 35.0%
TGATATGCGATTGACAATAC+AGG - Chr4:55777517-55777536 MsG0480021246.01.T01:intron 35.0%
TGATTAAGTGTGAGAATCTC+AGG + Chr4:55776717-55776736 None:intergenic 35.0%
TGTGAGTCGAATATAATTCC+AGG - Chr4:55777007-55777026 MsG0480021246.01.T01:five_prime_UTR 35.0%
TTTGAGAAATAGCTATCCTC+CGG - Chr4:55777750-55777769 MsG0480021246.01.T01:intron 35.0%
! AACTATTTTCATCACTCGTC+AGG + Chr4:55777816-55777835 None:intergenic 35.0%
! AAGGAGAGTGTGAAAATTGT+TGG + Chr4:55775483-55775502 None:intergenic 35.0%
! ATGCACTGACATAGTTAAAG+AGG + Chr4:55777302-55777321 None:intergenic 35.0%
! CATGATGATGAGGATTATCA+CGG - Chr4:55777976-55777995 MsG0480021246.01.T01:intron 35.0%
! CGATTTGTTCAAATCCCTAA+AGG + Chr4:55777399-55777418 None:intergenic 35.0%
! GTATGACAAACATGATGATG+AGG - Chr4:55777966-55777985 MsG0480021246.01.T01:intron 35.0%
! GTTAATGGATGTGTTAGTGA+CGG - Chr4:55775868-55775887 MsG0480021246.01.T01:CDS 35.0%
!! AAGAAGGGTATCAGGATTAA+AGG + Chr4:55776744-55776763 None:intergenic 35.0%
!! AGTTAACTTTGTTGTTGCAG+TGG - Chr4:55776467-55776486 MsG0480021246.01.T01:intron 35.0%
!! CCTCTTTTTGTCTGTTTTTC+AGG - Chr4:55776097-55776116 MsG0480021246.01.T01:intron 35.0%
!! TATTGGACTAGTGTTTGTCT+TGG - Chr4:55778079-55778098 MsG0480021246.01.T01:five_prime_UTR 35.0%
!! TTAACTTCAAGAAGGGTATC+AGG + Chr4:55776752-55776771 None:intergenic 35.0%
!! TTATCTTGGCTCTTTTTCTG+AGG - Chr4:55777684-55777703 MsG0480021246.01.T01:intron 35.0%
!!! GGTTTTCTGTTTGCTGTATA+AGG + Chr4:55775431-55775450 None:intergenic 35.0%
!!! TAGCTGTTTTTGGATCAAGA+GGG + Chr4:55778214-55778233 None:intergenic 35.0%
!!! TTAGCTGTTTTTGGATCAAG+AGG + Chr4:55778215-55778234 None:intergenic 35.0%
!!! TTTTGTCTGTTTTTCAGGTC+TGG - Chr4:55776102-55776121 MsG0480021246.01.T01:intron 35.0%
AACACCCTTATGTCTAACCA+CGG + Chr4:55776654-55776673 None:intergenic 40.0%
AAGATAAAGATGGCCGACTA+AGG + Chr4:55778238-55778257 None:intergenic 40.0%
AATAGTGTATCGCAGAACAG+TGG + Chr4:55777422-55777441 None:intergenic 40.0%
AGAATAGCCGTCTTCTGAAT+TGG + Chr4:55778156-55778175 None:intergenic 40.0%
ATTGTTGGAAGAGTAGAGTC+CGG + Chr4:55777066-55777085 None:intergenic 40.0%
CAACCTTCTCAAAAGGGATA+GGG - Chr4:55776807-55776826 MsG0480021246.01.T01:exon 40.0%
CAATTGAGGTCTATATTGCC+GGG - Chr4:55777082-55777101 MsG0480021246.01.T01:five_prime_UTR 40.0%
CGTATGCTGTTCTTGTGTAT+AGG + Chr4:55778002-55778021 None:intergenic 40.0%
CTCATATCAACCCTCGATAA+AGG - Chr4:55776620-55776639 MsG0480021246.01.T01:intron 40.0%
CTCTACTCTTCCAACAATTG+AGG - Chr4:55777068-55777087 MsG0480021246.01.T01:five_prime_UTR 40.0%
GCTATGGCATTGTACTTTAG+TGG - Chr4:55777469-55777488 MsG0480021246.01.T01:intron 40.0%
GGAAATGGACCAAGTGAAAA+TGG - Chr4:55776573-55776592 MsG0480021246.01.T01:intron 40.0%
GGAGTCGTTTGAATATGACA+TGG - Chr4:55777576-55777595 MsG0480021246.01.T01:intron 40.0%
GGATAGGGTGGATAGAAAAT+CGG - Chr4:55776822-55776841 MsG0480021246.01.T01:exon 40.0%
GGATTCCAACCTTCTCAAAA+GGG - Chr4:55776801-55776820 MsG0480021246.01.T01:CDS 40.0%
GTTACCGTGGTTAGACATAA+GGG - Chr4:55776647-55776666 MsG0480021246.01.T01:CDS 40.0%
GTTCTTAAATAGTGGCGCTA+TGG - Chr4:55777453-55777472 MsG0480021246.01.T01:intron 40.0%
TATTCCCCTACTTATTGAGG+TGG - Chr4:55778409-55778428 MsG0480021246.01.T01:five_prime_UTR 40.0%
TCCACGAGAAAGAAAAAGAG+AGG + Chr4:55775539-55775558 None:intergenic 40.0%
TCCAGGCACAAAGTTCTTTA+AGG - Chr4:55777024-55777043 MsG0480021246.01.T01:five_prime_UTR 40.0%
TCCTTAAAGAACTTTGTGCC+TGG + Chr4:55777028-55777047 None:intergenic 40.0%
TCTTTGGGCCAAGATAAAGA+TGG + Chr4:55778248-55778267 None:intergenic 40.0%
TTAGTCGGCCATCTTTATCT+TGG - Chr4:55778237-55778256 MsG0480021246.01.T01:five_prime_UTR 40.0%
! AGGTCTAGCGTTGCTTTAAT+TGG - Chr4:55777537-55777556 MsG0480021246.01.T01:intron 40.0%
! GGTTGTTATCGGTTATGTTG+AGG + Chr4:55775647-55775666 None:intergenic 40.0%
! TCCTCTCTTTTTCTTTCTCG+TGG - Chr4:55775535-55775554 MsG0480021246.01.T01:three_prime_UTR 40.0%
! TGTTATCGGTTATGTTGAGG+TGG + Chr4:55775644-55775663 None:intergenic 40.0%
!! CGACTAAGGTTAGCTGTTTT+TGG + Chr4:55778224-55778243 None:intergenic 40.0%
AAAGTAGAACTGTCTCGCGA+TGG - Chr4:55776530-55776549 MsG0480021246.01.T01:intron 45.0%
AAGGGGATCGATTACTTGCA+AGG + Chr4:55775412-55775431 None:intergenic 45.0%
AAGTGTGAGAATCTCAGGAG+TGG + Chr4:55776712-55776731 None:intergenic 45.0%
ACAAGAACAGCATACGCTGA+CGG - Chr4:55778006-55778025 MsG0480021246.01.T01:intron 45.0%
ACTATAGCGCTATGCCCTTT+AGG - Chr4:55777381-55777400 MsG0480021246.01.T01:intron 45.0%
AGGAGAAGCAATGTTGTTGC+CGG - Chr4:55777044-55777063 MsG0480021246.01.T01:five_prime_UTR 45.0%
AGTGCCGCAATTTGACAACA+CGG + Chr4:55777364-55777383 None:intergenic 45.0%
CCAACCTTCTCAAAAGGGAT+AGG - Chr4:55776806-55776825 MsG0480021246.01.T01:CDS 45.0%
CGCGATGGAGAATTCGTATA+AGG - Chr4:55776545-55776564 MsG0480021246.01.T01:intron 45.0%
CGTTACCGTGGTTAGACATA+AGG - Chr4:55776646-55776665 MsG0480021246.01.T01:CDS 45.0%
CTATAGCGCTATGCCCTTTA+GGG - Chr4:55777382-55777401 MsG0480021246.01.T01:intron 45.0%
GACTCCGTGTTGTCAAATTG+CGG - Chr4:55777357-55777376 MsG0480021246.01.T01:five_prime_UTR 45.0%
GACTGATGTCACAACCTAGT+AGG - Chr4:55777625-55777644 MsG0480021246.01.T01:intron 45.0%
GGAACTTTCATGGTTAACGC+CGG - Chr4:55778037-55778056 MsG0480021246.01.T01:intron 45.0%
GGGATTCCAACCTTCTCAAA+AGG - Chr4:55776800-55776819 MsG0480021246.01.T01:CDS 45.0%
GGTAACGAACCCTTTATCGA+GGG + Chr4:55776633-55776652 None:intergenic 45.0%
GTGGTAAGAGAAGATGTGGA+TGG + Chr4:55775625-55775644 None:intergenic 45.0%
GTTTGTCTTGGAATCGGCAT+TGG - Chr4:55778091-55778110 MsG0480021246.01.T01:five_prime_UTR 45.0%
TGAGGTGGTAAGAGAAGATG+TGG + Chr4:55775629-55775648 None:intergenic 45.0%
TGTCACAACCTAGTAGGAGA+TGG - Chr4:55777631-55777650 MsG0480021246.01.T01:intron 45.0%
! AAATCATACCAAGGTGCCAC+CGG - Chr4:55778130-55778149 MsG0480021246.01.T01:five_prime_UTR 45.0%
! ATGGTTAACGCCGGTTGTTA+GGG - Chr4:55778046-55778065 MsG0480021246.01.T01:intron 45.0%
! GAGTGGTAGTGTGAAGTTTG+AGG - Chr4:55776882-55776901 MsG0480021246.01.T01:five_prime_UTR 45.0%
! TCTGGAATTGCATTCAACGC+CGG + Chr4:55777180-55777199 None:intergenic 45.0%
!! CCTATCCCTTTTGAGAAGGT+TGG + Chr4:55776809-55776828 None:intergenic 45.0%
!! GTTAGGGTTGGTGTTGGTAT+TGG - Chr4:55778062-55778081 MsG0480021246.01.T01:five_prime_UTR 45.0%
CCAACACCAACCCTAACAAC+CGG + Chr4:55778059-55778078 None:intergenic 50.0%
CCTTCTCAAAAGGGATAGGG+TGG - Chr4:55776810-55776829 MsG0480021246.01.T01:exon 50.0%
CGATAAAGGGTTCGTTACCG+TGG - Chr4:55776634-55776653 MsG0480021246.01.T01:CDS 50.0%
CGGTAACGAACCCTTTATCG+AGG + Chr4:55776634-55776653 None:intergenic 50.0%
TAGCCGTCTTCTGAATTGGC+CGG + Chr4:55778152-55778171 None:intergenic 50.0%
TATATTGCCGGGTCCTTCTC+TGG - Chr4:55777093-55777112 MsG0480021246.01.T01:five_prime_UTR 50.0%
TGACAACACGGAGTCACACA+TGG + Chr4:55777352-55777371 None:intergenic 50.0%
TTGCATGTGTCGTGTTTCGC+CGG - Chr4:55775918-55775937 MsG0480021246.01.T01:intron 50.0%
! CATGGTTAACGCCGGTTGTT+AGG - Chr4:55778045-55778064 MsG0480021246.01.T01:intron 50.0%
! CTTGGAATCGGCATTGGAGT+TGG - Chr4:55778097-55778116 MsG0480021246.01.T01:five_prime_UTR 50.0%
! TTAACGCCGGTTGTTAGGGT+TGG - Chr4:55778050-55778069 MsG0480021246.01.T01:intron 50.0%
!! CCACCCTATCCCTTTTGAGA+AGG + Chr4:55776813-55776832 None:intergenic 50.0%
ACCTGCAAAGCAAGCGCAGA+AGG + Chr4:55777231-55777250 None:intergenic 55.0%
CCACCGGCCAATTCAGAAGA+CGG - Chr4:55778146-55778165 MsG0480021246.01.T01:five_prime_UTR 55.0%
GGCGAAGAGGGGAACTTTCA+TGG - Chr4:55778027-55778046 MsG0480021246.01.T01:intron 55.0%
TCCTTCTGCGCTTGCTTTGC+AGG - Chr4:55777227-55777246 MsG0480021246.01.T01:five_prime_UTR 55.0%
! GATGATCGGAGTGCCAGAGA+AGG + Chr4:55777109-55777128 None:intergenic 55.0%
!! CCGGTTGTTAGGGTTGGTGT+TGG - Chr4:55778056-55778075 MsG0480021246.01.T01:five_prime_UTR 55.0%
AGCATACGCTGACGGCGAAG+AGG - Chr4:55778014-55778033 MsG0480021246.01.T01:intron 60.0%
CATACGCTGACGGCGAAGAG+GGG - Chr4:55778016-55778035 MsG0480021246.01.T01:intron 60.0%
CCGTCTTCTGAATTGGCCGG+TGG + Chr4:55778149-55778168 None:intergenic 60.0%
GCATACGCTGACGGCGAAGA+GGG - Chr4:55778015-55778034 MsG0480021246.01.T01:intron 60.0%
TGAATTGGCCGGTGGCACCT+TGG + Chr4:55778141-55778160 None:intergenic 60.0%
GCCTGCATGTGCTCTCGAGC+CGG + Chr4:55777772-55777791 None:intergenic 65.0%
TGCATGTGCTCTCGAGCCGG+AGG + Chr4:55777769-55777788 None:intergenic 65.0%
! CGGAGTGCCAGAGAAGGACC+CGG + Chr4:55777103-55777122 None:intergenic 65.0%
! TCCGGCTCGAGAGCACATGC+AGG - Chr4:55777768-55777787 MsG0480021246.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 55775410 55778469 55775410 ID=MsG0480021246.01;Name=MsG0480021246.01
Chr4 mRNA 55775410 55778469 55775410 ID=MsG0480021246.01.T01;Parent=MsG0480021246.01;Name=MsG0480021246.01.T01;_AED=0.49;_eAED=0.49;_QI=981|1|1|1|0.5|0.33|3|416|98
Chr4 exon 55775410 55775927 55775410 ID=MsG0480021246.01.T01:exon:19024;Parent=MsG0480021246.01.T01
Chr4 exon 55776631 55777392 55776631 ID=MsG0480021246.01.T01:exon:19023;Parent=MsG0480021246.01.T01
Chr4 exon 55778056 55778469 55778056 ID=MsG0480021246.01.T01:exon:19022;Parent=MsG0480021246.01.T01
Chr4 five_prime_UTR 55778056 55778469 55778056 ID=MsG0480021246.01.T01:five_prime_utr;Parent=MsG0480021246.01.T01
Chr4 five_prime_UTR 55776826 55777392 55776826 ID=MsG0480021246.01.T01:five_prime_utr;Parent=MsG0480021246.01.T01
Chr4 CDS 55776631 55776825 55776631 ID=MsG0480021246.01.T01:cds;Parent=MsG0480021246.01.T01
Chr4 CDS 55775826 55775927 55775826 ID=MsG0480021246.01.T01:cds;Parent=MsG0480021246.01.T01
Chr4 three_prime_UTR 55775410 55775825 55775410 ID=MsG0480021246.01.T01:three_prime_utr;Parent=MsG0480021246.01.T01
Gene Sequence

>MsG0480021246.01.T01

ATGTTGTTGCCGGACTCTACTCTTCCAACAATTGAGGTCTATATTGCCGGGTCCTTCTCTGGCACTCCGATCATCTTAACGAAGACCTTGCATCTTAGTTCTCAAAAAAGACATACAAGGAAGCCGGCGTTGAATGCAATTCCAGAGAATGATGCAAATGAAAATGGGAAAGGTCCTTCTGCGCTTGCTTTGCAGGCTCCAGATTACATGTATGACAAACATGATGATGAGGATTATCACGGCCTATACACAAGAACAGCATACGCTGACGGCGAAGAGGGGAACTTTCATGGTTAA
Protein sequence

>MsG0480021246.01.T01

MLLPDSTLPTIEVYIAGSFSGTPIILTKTLHLSSQKRHTRKPALNAIPENDANENGKGPSALALQAPDYMYDKHDDEDYHGLYTRTAYADGEEGNFHG*