AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010204.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010204.01.T01 MTR_0271s0070 98.611 144 2 0 1 144 1 144 8.11e-103 290
MsG0280010204.01.T01 MTR_1g092420 98.611 144 2 0 1 144 1 144 2.04e-102 289
MsG0280010204.01.T01 MTR_2g013680 67.832 143 46 0 2 144 3 145 2.03e-68 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010204.01.T01 AT1G53035 62.500 144 54 0 1 144 7 150 7.10e-64 192
MsG0280010204.01.T01 AT1G53035 61.806 144 55 0 1 144 7 150 3.69e-63 191
MsG0280010204.01.T01 AT3G15358 61.111 144 56 0 1 144 1 144 3.91e-61 185

Find 26 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTGCTTTGGCTATTTCT+TGG 0.186754 2:+66164736 MsG0280010204.01.T01:CDS
GGATTATTGTTGTTCACAAT+TGG 0.247863 2:+66164552 MsG0280010204.01.T01:CDS
CAAACATCAGATTCCTTCTC+TGG 0.317012 2:+66164531 MsG0280010204.01.T01:CDS
GGTTGCTTGTGTTAAGGATA+TGG 0.337831 2:+66164590 MsG0280010204.01.T01:CDS
TCTTGCTTTGGCTATTTCTT+GGG 0.340551 2:+66164737 MsG0280010204.01.T01:CDS
TCTGAAAAGGTACATGTCTT+AGG 0.347459 2:+66164447 MsG0280010204.01.T01:CDS
GATTATTGTTGTTCACAATT+GGG 0.349528 2:+66164553 MsG0280010204.01.T01:CDS
TTCATATTTCAATGGCAGTT+TGG 0.351079 2:+66164649 MsG0280010204.01.T01:CDS
AATTGGGTTTCTTGTGTTCA+TGG 0.359752 2:+66164569 MsG0280010204.01.T01:CDS
TGTTGTTGGGGATCTTGCTT+TGG 0.362898 2:+66164725 MsG0280010204.01.T01:CDS
ATTGGCCTAGGCATGTTGTT+GGG 0.364725 2:+66164712 MsG0280010204.01.T01:CDS
TTTAACATGTGGATCATTGA+TGG 0.365683 2:+66164416 MsG0280010204.01.T01:CDS
TTGAAGAACTTGCTTGTGAT+TGG 0.405372 2:+66164694 MsG0280010204.01.T01:CDS
TTTCAGAGTTTCTTTGCTAA+AGG 0.414360 2:+66164615 MsG0280010204.01.T01:CDS
GTTCATGGTTGCTTGTGTTA+AGG 0.446942 2:+66164584 MsG0280010204.01.T01:CDS
TGTGTTTCTTCATATTTCAA+TGG 0.458849 2:+66164641 MsG0280010204.01.T01:CDS
AACTTGCTTGTGATTGGCCT+AGG 0.494396 2:+66164700 MsG0280010204.01.T01:CDS
GATTGGCCTAGGCATGTTGT+TGG 0.498297 2:+66164711 MsG0280010204.01.T01:CDS
TTGGCCTAGGCATGTTGTTG+GGG 0.550117 2:+66164713 MsG0280010204.01.T01:CDS
AAGACAGCAATTAAGCTACA+AGG 0.550968 2:+66164480 MsG0280010204.01.T01:CDS
TGAATTAATAATTGATCATG+TGG 0.571519 2:-66164510 None:intergenic
AGATCCCCAACAACATGCCT+AGG 0.575697 2:-66164717 None:intergenic
TCAACAATAGCTTTAACATG+TGG 0.588889 2:+66164405 MsG0280010204.01.T01:CDS
AAGGTACATGTCTTAGGACA+TGG 0.596575 2:+66164453 MsG0280010204.01.T01:CDS
GAACAACAATAATCCAGAGA+AGG 0.611024 2:-66164544 None:intergenic
AGACAGCAATTAAGCTACAA+GGG 0.677628 2:+66164481 MsG0280010204.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGAATTAATAATTGATCATG+TGG - Chr2:66164513-66164532 None:intergenic 20.0%
! TGTGTTTCTTCATATTTCAA+TGG + Chr2:66164641-66164660 MsG0280010204.01.T01:CDS 25.0%
!! GATTATTGTTGTTCACAATT+GGG + Chr2:66164553-66164572 MsG0280010204.01.T01:CDS 25.0%
!! TTTGGAGATTTTACTTTGTA+GGG + Chr2:66164667-66164686 MsG0280010204.01.T01:CDS 25.0%
!!! TGTTTTTTCTTGTGTTTATG+TGG + Chr2:66164760-66164779 MsG0280010204.01.T01:CDS 25.0%
TTCATATTTCAATGGCAGTT+TGG + Chr2:66164649-66164668 MsG0280010204.01.T01:CDS 30.0%
TTTAACATGTGGATCATTGA+TGG + Chr2:66164416-66164435 MsG0280010204.01.T01:CDS 30.0%
TTTCAGAGTTTCTTTGCTAA+AGG + Chr2:66164615-66164634 MsG0280010204.01.T01:CDS 30.0%
! GGATTATTGTTGTTCACAAT+TGG + Chr2:66164552-66164571 MsG0280010204.01.T01:CDS 30.0%
! GTTTGGAGATTTTACTTTGT+AGG + Chr2:66164666-66164685 MsG0280010204.01.T01:CDS 30.0%
! TTTTCTTGTGTTTATGTGGA+GGG + Chr2:66164764-66164783 MsG0280010204.01.T01:CDS 30.0%
! TTTTTCTTGTGTTTATGTGG+AGG + Chr2:66164763-66164782 MsG0280010204.01.T01:CDS 30.0%
!! TCAACAATAGCTTTAACATG+TGG + Chr2:66164405-66164424 MsG0280010204.01.T01:CDS 30.0%
!!! GATGGTTTTTTACTCTGAAA+AGG + Chr2:66164434-66164453 MsG0280010204.01.T01:CDS 30.0%
AAGACAGCAATTAAGCTACA+AGG + Chr2:66164480-66164499 MsG0280010204.01.T01:CDS 35.0%
AATTGGGTTTCTTGTGTTCA+TGG + Chr2:66164569-66164588 MsG0280010204.01.T01:CDS 35.0%
AGACAGCAATTAAGCTACAA+GGG + Chr2:66164481-66164500 MsG0280010204.01.T01:CDS 35.0%
GAACAACAATAATCCAGAGA+AGG - Chr2:66164547-66164566 None:intergenic 35.0%
! GAGATTTTACTTTGTAGGGA+AGG + Chr2:66164671-66164690 MsG0280010204.01.T01:CDS 35.0%
! TCTGAAAAGGTACATGTCTT+AGG + Chr2:66164447-66164466 MsG0280010204.01.T01:CDS 35.0%
! TTGAAGAACTTGCTTGTGAT+TGG + Chr2:66164694-66164713 MsG0280010204.01.T01:CDS 35.0%
!! ATCTTGCTTTGGCTATTTCT+TGG + Chr2:66164736-66164755 MsG0280010204.01.T01:CDS 35.0%
!! TCTTGCTTTGGCTATTTCTT+GGG + Chr2:66164737-66164756 MsG0280010204.01.T01:CDS 35.0%
AAGGTACATGTCTTAGGACA+TGG + Chr2:66164453-66164472 MsG0280010204.01.T01:CDS 40.0%
CAAACATCAGATTCCTTCTC+TGG + Chr2:66164531-66164550 MsG0280010204.01.T01:CDS 40.0%
GGTTGCTTGTGTTAAGGATA+TGG + Chr2:66164590-66164609 MsG0280010204.01.T01:CDS 40.0%
GTTCATGGTTGCTTGTGTTA+AGG + Chr2:66164584-66164603 MsG0280010204.01.T01:CDS 40.0%
TGTTGTTGGGGATCTTGCTT+TGG + Chr2:66164725-66164744 MsG0280010204.01.T01:CDS 45.0%
! ATTGGCCTAGGCATGTTGTT+GGG + Chr2:66164712-66164731 MsG0280010204.01.T01:CDS 45.0%
!! AACTTGCTTGTGATTGGCCT+AGG + Chr2:66164700-66164719 MsG0280010204.01.T01:CDS 45.0%
AGATCCCCAACAACATGCCT+AGG - Chr2:66164720-66164739 None:intergenic 50.0%
TTGGCCTAGGCATGTTGTTG+GGG + Chr2:66164713-66164732 MsG0280010204.01.T01:CDS 50.0%
!! GATTGGCCTAGGCATGTTGT+TGG + Chr2:66164711-66164730 MsG0280010204.01.T01:CDS 50.0%
Chromosome Type Strat End Strand Name
Chr2 gene 66164366 66164800 66164366 ID=MsG0280010204.01;Name=MsG0280010204.01
Chr2 mRNA 66164366 66164800 66164366 ID=MsG0280010204.01.T01;Parent=MsG0280010204.01;Name=MsG0280010204.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|144
Chr2 exon 66164366 66164800 66164366 ID=MsG0280010204.01.T01:exon:14249;Parent=MsG0280010204.01.T01
Chr2 CDS 66164366 66164800 66164366 ID=MsG0280010204.01.T01:cds;Parent=MsG0280010204.01.T01
Gene Sequence

>MsG0280010204.01.T01

ATGGGTTCATCTTGTTTTTTCTTAATATGTGTTCTTCATTCAACAATAGCTTTAACATGTGGATCATTGATGGTTTTTTACTCTGAAAAGGTACATGTCTTAGGACATGGAACAAAGACAGCAATTAAGCTACAAGGGTCAACACCACATGATCAATTATTAATTCAAACATCAGATTCCTTCTCTGGATTATTGTTGTTCACAATTGGGTTTCTTGTGTTCATGGTTGCTTGTGTTAAGGATATGGAGTTTCAGAGTTTCTTTGCTAAAGGATGTGTGTTTCTTCATATTTCAATGGCAGTTTGGAGATTTTACTTTGTAGGGAAGGTTGAAGAACTTGCTTGTGATTGGCCTAGGCATGTTGTTGGGGATCTTGCTTTGGCTATTTCTTGGGTGTTTTTTCTTGTGTTTATGTGGAGGGAGAAATATGATTAG

Protein sequence

>MsG0280010204.01.T01

MGSSCFFLICVLHSTIALTCGSLMVFYSEKVHVLGHGTKTAIKLQGSTPHDQLLIQTSDSFSGLLLFTIGFLVFMVACVKDMEFQSFFAKGCVFLHISMAVWRFYFVGKVEELACDWPRHVVGDLALAISWVFFLVFMWREKYD*