AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006411.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006411.01.T01 MTR_2g007950 92.063 63 5 0 6 68 11 73 1.83e-37 119
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006411.01.T01 AT1G25275 50.000 70 31 1 1 66 2 71 8.33e-18 70.5
MsG0280006411.01.T01 AT1G25275 50.000 70 31 1 1 66 2 71 1.12e-17 69.7
MsG0280006411.01.T01 AT1G25275 48.571 70 32 1 1 66 2 71 8.32e-17 67.8

Find 21 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGCTTCTGATTGCCTTGA+TGG 0.154913 2:-1587056 MsG0280006411.01.T01:CDS
CTCTTTGAACGCAGGCAGTT+TGG 0.316423 2:+1587030 None:intergenic
GCTACCTATTCAATGTTTGC+TGG 0.334029 2:-1586380 MsG0280006411.01.T01:three_prime_UTR
AACAACCAAGTTTAATTAGA+AGG 0.335558 2:+1586343 None:intergenic
ACAACCAGCAAACATTGAAT+AGG 0.448858 2:+1586376 None:intergenic
ATTGTGATAGCTAGCAGAAT+TGG 0.459069 2:+1586222 None:intergenic
ATGTAGCAAGTAATTGCTTA+AGG 0.470851 2:-1586255 MsG0280006411.01.T01:three_prime_UTR
TGCTCGATTAGATGCGGTCC+AGG 0.477134 2:-1586793 MsG0280006411.01.T01:intron
TATTACCTTCTAATTAAACT+TGG 0.478187 2:-1586348 MsG0280006411.01.T01:three_prime_UTR
TGCAGATTCTATGGTGGAGA+AGG 0.494670 2:-1586509 MsG0280006411.01.T01:intron
TGAGACAGTTGACAAGTTGT+TGG 0.503043 2:+1586440 None:intergenic
TCAAGGCAATCAGAAGCATC+AGG 0.516390 2:+1587059 None:intergenic
GTGACATGCAGCAAGGCTTA+CGG 0.519988 2:-1586833 MsG0280006411.01.T01:intron
GCAGATTCTATGGTGGAGAA+GGG 0.551697 2:-1586508 MsG0280006411.01.T01:intron
AGGCAGTTTGGCAACCATCA+AGG 0.564306 2:+1587042 None:intergenic
TTCTATGGTGGAGAAGGGCA+TGG 0.592128 2:-1586503 MsG0280006411.01.T01:CDS
ATGCAGCAAGGCTTACGGCA+AGG 0.642528 2:-1586828 MsG0280006411.01.T01:intron
CGCAAGTGCTCGATTAGATG+CGG 0.643345 2:-1586799 MsG0280006411.01.T01:CDS
CTAACAGTCTCTTTGAACGC+AGG 0.676017 2:+1587022 None:intergenic
GAAGATGATGAAGAAGACGA+CGG 0.724386 2:-1587135 MsG0280006411.01.T01:exon
GATGATGAAGAAGACGACGG+TGG 0.786514 2:-1587132 MsG0280006411.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATTAAAAAAGAAAAAGAAA+TGG + Chr2:1586674-1586693 None:intergenic 15.0%
!! GAATTATTAAGAAAAGTAAA+AGG + Chr2:1586437-1586456 None:intergenic 15.0%
!!! AAAAGAAATGGTATTATTTT+TGG + Chr2:1586662-1586681 None:intergenic 15.0%
!!! AAAGAAATGGTATTATTTTT+GGG + Chr2:1586661-1586680 None:intergenic 15.0%
!!! TCTTTTTCTTTTTTAATGAA+AGG - Chr2:1586676-1586695 MsG0280006411.01.T01:intron 15.0%
!! CAAGAAATTTAATTAAACCT+CGG + Chr2:1586744-1586763 None:intergenic 20.0%
!! TATTACCTTCTAATTAAACT+TGG - Chr2:1587068-1587087 MsG0280006411.01.T01:CDS 20.0%
!!! ATTTAATTTTGCAGATTCTA+TGG - Chr2:1586898-1586917 MsG0280006411.01.T01:intron 20.0%
!!! ATTTGTGATATTTTCTCAAA+TGG - Chr2:1586323-1586342 MsG0280006411.01.T01:three_prime_UTR 20.0%
!!! CATTGTCTTCTTTTAATTTA+AGG - Chr2:1586456-1586475 MsG0280006411.01.T01:three_prime_UTR 20.0%
! AACAACCAAGTTTAATTAGA+AGG + Chr2:1587076-1587095 None:intergenic 25.0%
ATGTAGCAAGTAATTGCTTA+AGG - Chr2:1587161-1587180 MsG0280006411.01.T01:five_prime_UTR 30.0%
TTTGGGTAATGTTAATTACC+TGG + Chr2:1586644-1586663 None:intergenic 30.0%
! TAATTTTGCAGATTCTATGG+TGG - Chr2:1586901-1586920 MsG0280006411.01.T01:intron 30.0%
ACAACCAGCAAACATTGAAT+AGG + Chr2:1587043-1587062 None:intergenic 35.0%
ATAATATATTGTCTGAGCCC+AGG + Chr2:1586845-1586864 None:intergenic 35.0%
CTTGAATGATCGAAAACAAC+AGG - Chr2:1586760-1586779 MsG0280006411.01.T01:intron 35.0%
! CTCTTTTCTCTTTGATCGTT+TGG + Chr2:1586247-1586266 None:intergenic 35.0%
ATGATCGAAAACAACAGGTC+TGG - Chr2:1586765-1586784 MsG0280006411.01.T01:intron 40.0%
GAAGATGATGAAGAAGACGA+CGG - Chr2:1586281-1586300 MsG0280006411.01.T01:three_prime_UTR 40.0%
GCTACCTATTCAATGTTTGC+TGG - Chr2:1587036-1587055 MsG0280006411.01.T01:CDS 40.0%
TATGTATGTGACATGCAGCA+AGG - Chr2:1586576-1586595 MsG0280006411.01.T01:intron 40.0%
TGAGACAGTTGACAAGTTGT+TGG + Chr2:1586979-1586998 None:intergenic 40.0%
TGATCGAAAACAACAGGTCT+GGG - Chr2:1586766-1586785 MsG0280006411.01.T01:intron 40.0%
! TTAGTGCTGAAAAGATACCG+AGG - Chr2:1586724-1586743 MsG0280006411.01.T01:intron 40.0%
CTAACAGTCTCTTTGAACGC+AGG + Chr2:1586397-1586416 None:intergenic 45.0%
GATGCTTCTGATTGCCTTGA+TGG - Chr2:1586360-1586379 MsG0280006411.01.T01:three_prime_UTR 45.0%
! GCAGATTCTATGGTGGAGAA+GGG - Chr2:1586908-1586927 MsG0280006411.01.T01:intron 45.0%
! TGCAGATTCTATGGTGGAGA+AGG - Chr2:1586907-1586926 MsG0280006411.01.T01:intron 45.0%
!! TCAAGGCAATCAGAAGCATC+AGG + Chr2:1586360-1586379 None:intergenic 45.0%
AGGCAGTTTGGCAACCATCA+AGG + Chr2:1586377-1586396 None:intergenic 50.0%
CGCAAGTGCTCGATTAGATG+CGG - Chr2:1586617-1586636 MsG0280006411.01.T01:intron 50.0%
CTCTTTGAACGCAGGCAGTT+TGG + Chr2:1586389-1586408 None:intergenic 50.0%
GATGATGAAGAAGACGACGG+TGG - Chr2:1586284-1586303 MsG0280006411.01.T01:three_prime_UTR 50.0%
GTGACATGCAGCAAGGCTTA+CGG - Chr2:1586583-1586602 MsG0280006411.01.T01:intron 50.0%
TCGAAAACAACAGGTCTGGG+TGG - Chr2:1586769-1586788 MsG0280006411.01.T01:intron 50.0%
! ACGCTAGTCTCACACACTAG+AGG - Chr2:1586806-1586825 MsG0280006411.01.T01:CDS 50.0%
! TTCTATGGTGGAGAAGGGCA+TGG - Chr2:1586913-1586932 MsG0280006411.01.T01:intron 50.0%
ACAGGTCTGGGTGGTGTAGT+CGG - Chr2:1586778-1586797 MsG0280006411.01.T01:intron 55.0%
ATGCAGCAAGGCTTACGGCA+AGG - Chr2:1586588-1586607 MsG0280006411.01.T01:intron 55.0%
TCTGAGCCCAGGTTCGAACT+GGG + Chr2:1586834-1586853 None:intergenic 55.0%
TGCTCGATTAGATGCGGTCC+AGG - Chr2:1586623-1586642 MsG0280006411.01.T01:intron 55.0%
AGAGGTCCCCAGTTCGAACC+TGG - Chr2:1586824-1586843 MsG0280006411.01.T01:CDS 60.0%
CTGAGCCCAGGTTCGAACTG+GGG + Chr2:1586833-1586852 None:intergenic 60.0%
GAGGTCCCCAGTTCGAACCT+GGG - Chr2:1586825-1586844 MsG0280006411.01.T01:CDS 60.0%
GTCTGAGCCCAGGTTCGAAC+TGG + Chr2:1586835-1586854 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 1586225 1587213 1586225 ID=MsG0280006411.01;Name=MsG0280006411.01
Chr2 mRNA 1586225 1587213 1586225 ID=MsG0280006411.01.T01;Parent=MsG0280006411.01;Name=MsG0280006411.01.T01;_AED=0.50;_eAED=0.50;_QI=60|1|1|1|0|0|3|273|68
Chr2 exon 1586225 1586526 1586225 ID=MsG0280006411.01.T01:exon:182;Parent=MsG0280006411.01.T01
Chr2 exon 1586794 1586844 1586794 ID=MsG0280006411.01.T01:exon:181;Parent=MsG0280006411.01.T01
Chr2 exon 1587027 1587213 1587027 ID=MsG0280006411.01.T01:exon:180;Parent=MsG0280006411.01.T01
Chr2 five_prime_UTR 1587154 1587213 1587154 ID=MsG0280006411.01.T01:five_prime_utr;Parent=MsG0280006411.01.T01
Chr2 CDS 1587027 1587153 1587027 ID=MsG0280006411.01.T01:cds;Parent=MsG0280006411.01.T01
Chr2 CDS 1586794 1586844 1586794 ID=MsG0280006411.01.T01:cds;Parent=MsG0280006411.01.T01
Chr2 CDS 1586498 1586526 1586498 ID=MsG0280006411.01.T01:cds;Parent=MsG0280006411.01.T01
Chr2 three_prime_UTR 1586225 1586497 1586225 ID=MsG0280006411.01.T01:three_prime_utr;Parent=MsG0280006411.01.T01
Gene Sequence

>MsG0280006411.01.T01

ATGATGAAGAAGACGACGGTGGTTGCTATGATGCTTTTATTTGTGATATTTTCTCAAATGGATAGCGTTGTTCCTGATGCTTCTGATTGCCTTGATGGTTGCCAAACTGCCTGCGTTCAAAGAGACTCAAGGCTTACGGCAAGGTGTGAGCGCAAGTGCTCGATTAGATGCGGTCCAGATTCTATGGTGGAGAAGGGCATGGATTGA

Protein sequence

>MsG0280006411.01.T01

MMKKTTVVAMMLLFVIFSQMDSVVPDASDCLDGCQTACVQRDSRLTARCERKCSIRCGPDSMVEKGMD*