AlfalfaGEDB | MsG0480021369.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021369.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021369.01.T01	PNY03825.1	89.13	92	10	0	1	92	1	92	6.76E-56	177

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021369.01.T01	P33083	76.667	90	21	0	1	90	1	90	2.05E-47	149

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021369.01.T01	A0A2K3NLB0	89.130	92	10	0	1	92	1	92	3.23e-56	177

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000782.01	MsG0480021369.01	0.808087	3.678410e-50	2.083912e-47
MsG0480020890.01	MsG0480021369.01	0.820067	8.343078e-53	6.534499e-50
MsG0480021369.01	MsG0480021370.01	0.831912	1.275265e-55	1.403648e-52
MsG0480021369.01	MsG0480021381.01	0.804482	2.115097e-49	1.091757e-46
MsG0480021369.01	MsG0480022355.01	0.803126	4.044591e-49	2.016220e-46
MsG0480021369.01	MsG0780036979.01	0.808156	3.556049e-50	2.018344e-47

PPI

Gene1	Gene2	Type
MsG0480021386.01	MsG0480021369.01	PPI
MsG0480021369.01	MsG0480021386.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021369.01.T01	MTR_4g072570	91.304	92	8	0	1	92	1	92	3.57e-58	173
MsG0480021369.01.T01	MTR_4g072680	85.870	92	13	0	1	92	1	92	5.38e-57	170
MsG0480021369.01.T01	MTR_4g072620	84.783	92	14	0	1	92	1	92	5.75e-57	170
MsG0480021369.01.T01	MTR_4g072480	84.783	92	14	0	1	92	1	92	1.60e-56	169
MsG0480021369.01.T01	MTR_4g072730	83.516	91	15	0	1	91	1	91	2.06e-55	166
MsG0480021369.01.T01	MTR_4g072460	83.696	92	15	0	1	92	1	92	2.27e-55	166
MsG0480021369.01.T01	MTR_4g072500	81.522	92	17	0	1	92	1	92	6.72e-52	157
MsG0480021369.01.T01	MTR_4g072310	78.495	93	19	1	1	92	1	93	3.36e-51	155
MsG0480021369.01.T01	MTR_3g084150	78.261	92	20	0	1	92	1	92	2.23e-50	154
MsG0480021369.01.T01	MTR_4g072530	80.000	90	18	0	1	90	1	90	1.58e-49	151
MsG0480021369.01.T01	MTR_3g084180	77.174	92	21	0	1	92	1	92	2.81e-49	151
MsG0480021369.01.T01	MTR_4g072660	77.174	92	16	1	1	92	1	87	3.71e-49	150
MsG0480021369.01.T01	MTR_8g461410	76.087	92	22	0	1	92	1	92	6.33e-49	150
MsG0480021369.01.T01	MTR_4g072880	73.118	93	24	1	1	92	1	93	1.64e-48	149
MsG0480021369.01.T01	MTR_4g072230	78.495	93	17	2	1	92	1	91	4.29e-48	148
MsG0480021369.01.T01	MTR_4g072640	78.889	90	19	0	1	90	1	90	4.77e-48	148
MsG0480021369.01.T01	MTR_4g072250	77.419	93	19	2	1	92	1	92	1.94e-47	146
MsG0480021369.01.T01	MTR_4g072270	73.118	93	24	1	1	92	1	93	1.07e-46	144
MsG0480021369.01.T01	MTR_3g084240	72.826	92	25	0	1	92	1	92	3.80e-46	143
MsG0480021369.01.T01	MTR_3g084170	72.826	92	25	0	1	92	1	92	7.11e-46	142
MsG0480021369.01.T01	MTR_4g072860	75.269	93	22	1	1	92	1	93	7.91e-46	142
MsG0480021369.01.T01	MTR_5g008360	83.750	80	13	0	13	92	4	83	2.88e-45	140
MsG0480021369.01.T01	MTR_3g117630	80.723	83	16	0	10	92	10	92	3.81e-45	140
MsG0480021369.01.T01	MTR_3g110032	73.118	93	24	1	1	92	1	93	1.24e-44	139
MsG0480021369.01.T01	MTR_4g072240	69.231	91	27	1	1	90	1	91	2.00e-44	139
MsG0480021369.01.T01	MTR_3g084250	71.739	92	26	0	1	92	1	92	2.02e-44	139
MsG0480021369.01.T01	MTR_4g050700	72.043	93	25	1	1	92	1	93	4.74e-44	138
MsG0480021369.01.T01	MTR_2g012410	72.043	93	25	1	1	92	1	93	4.74e-44	138
MsG0480021369.01.T01	MTR_3g110042	75.281	89	22	0	1	89	1	89	6.40e-44	137
MsG0480021369.01.T01	MTR_4g072370	74.444	90	21	1	1	90	1	88	7.66e-44	137
MsG0480021369.01.T01	MTR_3g084220	69.565	92	28	0	1	92	1	92	9.59e-44	137
MsG0480021369.01.T01	MTR_4g072330	72.222	90	23	1	1	90	1	88	1.99e-43	136
MsG0480021369.01.T01	MTR_4g072300	67.033	91	29	1	1	90	1	91	3.18e-43	135
MsG0480021369.01.T01	MTR_4g072220	67.742	93	29	1	1	92	1	93	4.02e-43	135
MsG0480021369.01.T01	MTR_3g084260	70.968	93	26	1	1	92	1	93	1.09e-42	134
MsG0480021369.01.T01	MTR_3g110038	68.478	92	29	0	1	92	1	92	1.29e-42	134
MsG0480021369.01.T01	MTR_4g072870	68.132	91	28	1	1	90	1	91	1.58e-42	134
MsG0480021369.01.T01	MTR_4g072580	72.222	90	20	1	1	90	1	85	1.71e-42	134
MsG0480021369.01.T01	MTR_4g072850	71.277	94	25	1	1	92	1	94	2.56e-42	133
MsG0480021369.01.T01	MTR_4g072550	80.822	73	14	0	20	92	31	103	8.33e-42	132
MsG0480021369.01.T01	MTR_4g072470	73.333	90	19	1	1	90	1	85	1.65e-41	131
MsG0480021369.01.T01	MTR_4g072830	71.910	89	23	1	1	89	1	87	2.48e-41	130
MsG0480021369.01.T01	MTR_4g072740	71.111	90	21	1	1	90	1	85	4.28e-41	130
MsG0480021369.01.T01	MTR_4g072490	68.889	90	23	1	1	90	1	85	7.17e-41	129
MsG0480021369.01.T01	MTR_5g008370	83.784	74	12	0	18	91	18	91	7.26e-41	131
MsG0480021369.01.T01	MTR_3g084210	70.652	92	27	0	1	92	1	92	2.12e-40	129
MsG0480021369.01.T01	MTR_4g072890	64.835	91	31	1	1	90	1	91	2.45e-40	128
MsG0480021369.01.T01	MTR_4g072260	63.441	93	33	1	1	92	1	93	3.46e-40	128
MsG0480021369.01.T01	MTR_4g072600	71.111	90	21	1	1	90	1	85	1.89e-39	126
MsG0480021369.01.T01	MTR_4g072940	81.429	70	13	0	21	90	53	122	3.01e-39	127
MsG0480021369.01.T01	MTR_4g072610	70.000	90	21	1	1	90	1	84	4.98e-39	125
MsG0480021369.01.T01	MTR_4g072630	65.556	90	27	1	1	90	1	86	7.64e-39	124
MsG0480021369.01.T01	MTR_3g084190	67.391	92	30	0	1	92	1	92	3.11e-38	123
MsG0480021369.01.T01	MTR_8g461420	68.889	90	28	0	1	90	1	90	4.77e-38	122
MsG0480021369.01.T01	MTR_4g072540	68.889	90	24	1	1	90	1	86	5.21e-38	122
MsG0480021369.01.T01	MTR_4g072465	65.556	90	27	1	1	90	1	86	9.95e-38	121
MsG0480021369.01.T01	MTR_3g084230	68.889	90	28	0	1	90	1	90	2.34e-37	121
MsG0480021369.01.T01	MTR_4g072820	68.132	91	22	2	1	90	1	85	6.01e-37	119
MsG0480021369.01.T01	MTR_4g072720	68.889	90	24	1	1	90	1	86	7.09e-37	119
MsG0480021369.01.T01	MTR_4g072650	66.667	90	24	1	1	90	1	84	8.08e-37	119
MsG0480021369.01.T01	MTR_3g084160	66.304	92	31	0	1	92	1	92	9.73e-37	119
MsG0480021369.01.T01	MTR_4g072670	68.889	90	24	1	1	90	1	86	1.11e-36	119
MsG0480021369.01.T01	MTR_3g110045	70.000	90	27	0	3	92	62	151	2.34e-36	120
MsG0480021369.01.T01	MTR_4g072590	70.000	90	22	1	1	90	1	85	2.99e-35	115
MsG0480021369.01.T01	MTR_4g072510	65.556	90	27	1	1	90	1	86	8.73e-35	114
MsG0480021369.01.T01	MTR_4g072290	62.366	93	20	2	1	92	1	79	1.00e-33	111
MsG0480021369.01.T01	MTR_4g072380	64.835	91	26	2	1	90	1	86	1.07e-33	111
MsG0480021369.01.T01	MTR_4g072690	68.889	90	23	1	1	90	1	85	2.20e-33	110
MsG0480021369.01.T01	MTR_4g072750	74.324	74	17	1	1	72	1	74	2.43e-33	110
MsG0480021369.01.T01	MTR_4g072450	68.889	90	23	1	1	90	1	85	1.64e-32	108
MsG0480021369.01.T01	MTR_5g008350	65.823	79	22	1	1	79	1	74	2.41e-32	107
MsG0480021369.01.T01	MTR_4g072910	62.069	87	30	2	4	90	17	100	4.48e-32	108
MsG0480021369.01.T01	MTR_4g072770	75.758	66	16	0	25	90	15	80	6.86e-32	107
MsG0480021369.01.T01	MTR_4g072980	56.842	95	38	3	1	92	1	95	9.88e-32	107
MsG0480021369.01.T01	MTR_4g072560	52.577	97	38	2	1	89	1	97	2.12e-31	106
MsG0480021369.01.T01	MTR_3g117640	69.565	69	20	1	24	91	33	101	1.17e-30	104
MsG0480021369.01.T01	MTR_3g110035	68.657	67	21	0	26	92	72	138	1.74e-30	105
MsG0480021369.01.T01	MTR_3g084200	65.278	72	25	0	1	72	1	72	3.98e-30	103
MsG0480021369.01.T01	MTR_6g077850	58.209	67	28	0	24	90	33	99	5.22e-26	92.8
MsG0480021369.01.T01	MTR_4g072280	58.667	75	30	1	1	74	1	75	1.38e-25	90.9
MsG0480021369.01.T01	MTR_3g113310	58.209	67	27	1	23	89	40	105	6.26e-25	90.1
MsG0480021369.01.T01	MTR_4g072190	56.338	71	30	1	19	89	30	99	6.92e-24	87.4
MsG0480021369.01.T01	MTR_3g117610	61.538	65	24	1	23	87	43	106	7.03e-24	87.4
MsG0480021369.01.T01	MTR_4g072930	53.086	81	33	2	15	90	30	110	1.50e-22	84.3
MsG0480021369.01.T01	MTR_8g461400	47.826	92	22	1	1	92	1	66	1.20e-21	80.5
MsG0480021369.01.T01	MTR_5g021820	54.545	66	29	1	24	89	39	103	1.13e-20	79.3
MsG0480021369.01.T01	MTR_8g076040	56.250	64	27	1	24	87	40	102	1.88e-20	78.6
MsG0480021369.01.T01	MTR_4g072840	45.055	91	18	3	1	90	1	60	6.27e-17	68.6
MsG0480021369.01.T01	MTR_2g043960	49.275	69	34	1	17	85	68	135	8.00e-17	70.9
MsG0480021369.01.T01	MTR_4g124750	51.562	64	30	1	24	87	68	130	1.16e-16	70.5
MsG0480021369.01.T01	MTR_4g005310	48.052	77	34	2	15	85	47	123	1.48e-16	70.1
MsG0480021369.01.T01	MTR_8g461210	43.333	90	48	2	4	91	14	102	2.86e-16	68.6
MsG0480021369.01.T01	MTR_3g092220	41.176	85	44	3	1	83	12	92	3.97e-16	67.8
MsG0480021369.01.T01	MTR_1g094750	43.529	85	43	3	5	85	26	109	8.16e-16	67.4
MsG0480021369.01.T01	MTR_1g009150	51.471	68	32	1	18	85	64	130	1.32e-15	68.2
MsG0480021369.01.T01	MTR_7g118010	47.059	68	33	2	25	90	23	89	1.61e-15	66.2
MsG0480021369.01.T01	MTR_1g024085	45.783	83	41	2	4	83	13	94	5.51e-15	65.9
MsG0480021369.01.T01	MTR_0388s0020	43.373	83	42	3	10	89	61	141	8.81e-15	65.5
MsG0480021369.01.T01	MTR_8g026730	45.588	68	34	2	25	90	27	93	1.01e-14	64.3
MsG0480021369.01.T01	MTR_3g109160	43.750	80	44	1	12	91	56	134	2.12e-14	64.7
MsG0480021369.01.T01	MTR_1g061570	47.059	68	35	1	24	91	18	84	2.18e-14	63.9
MsG0480021369.01.T01	MTR_4g025830	44.118	68	34	2	25	89	79	145	2.32e-14	64.7
MsG0480021369.01.T01	MTR_4g005320	50.820	61	29	1	25	85	70	129	3.53e-14	64.3
MsG0480021369.01.T01	MTR_4g072160	47.887	71	34	2	16	85	28	96	5.89e-14	63.2
MsG0480021369.01.T01	MTR_8g101520	40.476	84	49	1	7	90	32	114	8.41e-14	63.2
MsG0480021369.01.T01	MTR_3g097120	42.308	78	36	2	20	89	4	80	9.74e-14	61.6
MsG0480021369.01.T01	MTR_1g059770	41.935	62	35	1	28	89	52	112	8.70e-13	59.7
MsG0480021369.01.T01	MTR_3g105900	75.000	36	9	0	24	59	1171	1206	1.45e-12	62.0
MsG0480021369.01.T01	MTR_2g066830	37.349	83	51	1	8	90	41	122	1.52e-12	59.3
MsG0480021369.01.T01	MTR_8g101530	39.437	71	42	1	19	89	34	103	1.66e-12	59.3
MsG0480021369.01.T01	MTR_7g104940	37.333	75	42	2	15	89	44	113	1.71e-12	58.9
MsG0480021369.01.T01	MTR_1g063950	43.939	66	36	1	17	82	40	104	1.89e-12	58.9
MsG0480021369.01.T01	MTR_8g094980	40.000	80	34	2	23	89	1	79	2.18e-12	58.2
MsG0480021369.01.T01	MTR_7g101740	46.875	64	33	1	24	87	39	101	2.79e-12	58.2
MsG0480021369.01.T01	MTR_2g095430	50.000	58	24	2	24	77	44	100	3.30e-12	58.2
MsG0480021369.01.T01	MTR_4g021810	51.613	62	28	2	25	85	26	86	3.62e-12	57.8
MsG0480021369.01.T01	MTR_3g098970	32.051	78	52	1	15	92	43	119	5.04e-12	58.2
MsG0480021369.01.T01	MTR_7g104950	34.211	76	45	2	14	89	43	113	6.70e-12	57.4
MsG0480021369.01.T01	MTR_8g096470	46.552	58	30	1	28	85	49	105	7.84e-12	57.0
MsG0480021369.01.T01	MTR_1g063310	42.105	76	41	2	17	90	18	92	1.57e-11	55.8
MsG0480021369.01.T01	MTR_7g405740	45.763	59	31	1	27	85	69	126	3.93e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021369.01.T01	AT4G34770	61.111	90	33	1	4	91	14	103	2.05e-33	111
MsG0480021369.01.T01	AT5G18060	58.427	89	32	2	7	90	2	90	4.41e-33	110
MsG0480021369.01.T01	AT4G38840	70.588	68	19	1	24	90	32	99	6.03e-33	110
MsG0480021369.01.T01	AT5G18080	59.091	88	31	2	7	89	2	89	1.44e-32	108
MsG0480021369.01.T01	AT2G21200	60.000	85	32	2	7	89	2	86	2.20e-32	108
MsG0480021369.01.T01	AT5G18020	59.091	88	31	2	7	89	2	89	2.62e-32	108
MsG0480021369.01.T01	AT5G18030	58.621	87	33	2	7	90	2	88	3.60e-32	107
MsG0480021369.01.T01	AT5G18050	57.955	88	32	2	7	89	2	89	4.86e-32	107
MsG0480021369.01.T01	AT2G21210	51.579	95	41	2	1	90	1	95	5.06e-31	105
MsG0480021369.01.T01	AT3G03840	64.384	73	24	1	21	91	23	95	6.59e-31	105
MsG0480021369.01.T01	AT5G18010	56.180	89	34	2	7	90	2	90	1.52e-30	103
MsG0480021369.01.T01	AT4G38825	61.333	75	28	1	17	90	15	89	1.77e-30	103
MsG0480021369.01.T01	AT2G21210	51.579	95	41	2	1	90	68	162	2.41e-30	105
MsG0480021369.01.T01	AT3G03850	70.149	67	18	1	27	91	27	93	7.01e-30	102
MsG0480021369.01.T01	AT4G34800	63.014	73	24	1	13	82	9	81	1.08e-29	102
MsG0480021369.01.T01	AT4G38850	55.682	88	35	2	7	90	2	89	3.03e-29	100
MsG0480021369.01.T01	AT4G34810	68.571	70	17	2	25	89	48	117	1.50e-28	99.8
MsG0480021369.01.T01	AT3G03820	57.143	77	30	1	18	91	20	96	2.86e-28	98.2
MsG0480021369.01.T01	AT3G03830	50.549	91	38	2	7	90	2	92	1.14e-26	94.0
MsG0480021369.01.T01	AT4G34780	65.714	70	21	1	23	89	26	95	2.14e-26	94.0
MsG0480021369.01.T01	AT4G13790	49.367	79	38	1	13	89	13	91	8.00e-26	92.0
MsG0480021369.01.T01	AT4G34790	61.538	65	23	1	25	87	39	103	1.41e-23	86.7
MsG0480021369.01.T01	AT1G75580	52.500	80	37	1	10	89	29	107	1.81e-23	86.7
MsG0480021369.01.T01	AT4G34760	58.209	67	27	1	23	89	41	106	4.42e-23	85.5
MsG0480021369.01.T01	AT4G38860	55.882	68	30	0	23	90	38	105	6.55e-23	85.1
MsG0480021369.01.T01	AT2G21220	56.716	67	28	1	23	89	38	103	1.14e-22	84.3
MsG0480021369.01.T01	AT1G19830	56.164	73	30	2	18	89	41	112	7.92e-20	77.4
MsG0480021369.01.T01	AT2G16580	53.731	67	30	1	23	89	42	107	8.79e-20	77.0
MsG0480021369.01.T01	AT4G36110	54.688	64	28	1	24	87	39	101	6.22e-19	74.7
MsG0480021369.01.T01	AT2G18010	53.125	64	29	1	24	87	47	109	4.60e-18	72.8
MsG0480021369.01.T01	AT3G20220	53.846	65	28	2	25	88	47	110	7.32e-18	72.4
MsG0480021369.01.T01	AT3G43120	47.222	72	37	1	14	85	68	138	1.31e-17	73.2
MsG0480021369.01.T01	AT2G46690	40.230	87	48	2	8	91	7	92	5.38e-17	70.5
MsG0480021369.01.T01	AT5G66260	53.030	66	30	1	25	90	34	98	7.94e-17	69.3
MsG0480021369.01.T01	AT5G20810	49.275	69	34	1	17	85	71	138	3.22e-16	70.1
MsG0480021369.01.T01	AT5G20810	53.226	62	28	1	24	85	78	138	3.23e-16	69.7
MsG0480021369.01.T01	AT3G51200	45.205	73	38	2	10	82	26	96	7.07e-16	67.0
MsG0480021369.01.T01	AT1G75590	50.685	73	31	2	15	83	28	99	2.02e-15	67.4
MsG0480021369.01.T01	AT5G10990	51.667	60	28	1	24	83	40	98	7.38e-15	65.5
MsG0480021369.01.T01	AT3G61900	44.286	70	35	2	24	90	29	97	1.20e-14	64.7
MsG0480021369.01.T01	AT4G34750	45.312	64	34	1	24	87	36	98	2.32e-14	64.3
MsG0480021369.01.T01	AT4G34750	45.312	64	34	1	24	87	36	98	2.32e-14	64.3
MsG0480021369.01.T01	AT1G19840	46.250	80	39	2	4	83	23	98	2.44e-14	64.3
MsG0480021369.01.T01	AT3G12830	41.379	87	49	2	4	90	30	114	3.27e-14	63.5
MsG0480021369.01.T01	AT4G00880	47.826	69	33	2	24	90	27	94	8.49e-14	62.4
MsG0480021369.01.T01	AT3G60690	43.056	72	37	2	21	89	84	154	1.33e-13	62.8
MsG0480021369.01.T01	AT5G53590	43.056	72	35	2	24	90	45	115	1.41e-13	62.0
MsG0480021369.01.T01	AT2G45210	39.726	73	40	2	20	89	74	145	1.69e-13	62.4
MsG0480021369.01.T01	AT4G31320	50.820	61	29	1	25	85	80	139	1.72e-13	62.8
MsG0480021369.01.T01	AT2G24400	52.459	61	28	1	25	85	64	123	3.57e-13	62.0
MsG0480021369.01.T01	AT2G37030	50.847	59	28	1	24	82	48	105	5.56e-13	60.1
MsG0480021369.01.T01	AT3G09870	48.571	70	33	2	18	85	38	106	7.80e-13	59.7
MsG0480021369.01.T01	AT1G76190	48.333	60	29	2	26	85	22	79	7.69e-12	57.4
MsG0480021369.01.T01	AT1G56150	45.455	66	35	1	25	90	38	102	8.57e-12	57.0
MsG0480021369.01.T01	AT3G03847	64.286	42	14	1	41	82	87	127	1.05e-11	57.0
MsG0480021369.01.T01	AT4G22620	33.708	89	55	2	4	89	58	145	1.06e-11	57.8
MsG0480021369.01.T01	AT4G12410	41.176	68	36	2	25	89	76	142	1.90e-11	57.0
MsG0480021369.01.T01	AT1G20470	46.154	65	33	2	21	85	19	81	2.04e-11	56.6
MsG0480021369.01.T01	AT4G09530	42.647	68	36	2	19	83	22	89	2.80e-11	55.5
MsG0480021369.01.T01	AT1G16510	44.286	70	36	2	25	92	54	122	4.21e-11	55.8
MsG0480021369.01.T01	AT2G36210	38.356	73	45	0	15	87	44	116	5.22e-11	55.5

Find 24 sgRNAs with CRISPR-Local

Find 24 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCGTTTACATGCTACTCTA+AGG	0.308242	4:-57163878	MsG0480021369.01.T01:CDS
TGGTTGGTTCAAGTATGATA+TGG	0.316740	4:+57163755	MsG0480021370.01.T01:intron
CATATAACATCTCGCTTGAA+TGG	0.355967	4:-57163636	MsG0480021369.01.T01:CDS
AGTCAAGCTGAGGAAGAGTT+TGG	0.376098	4:-57163714	MsG0480021369.01.T01:CDS
GGTTGGTTCAAGTATGATAT+GGG	0.445606	4:+57163756	MsG0480021370.01.T01:intron
CCCTTAGAGTAGCATGTAAA+CGG	0.454025	4:+57163877	MsG0480021370.01.T01:intron
AAAATCTGTAGAAGTGAGAA+AGG	0.471464	4:-57163826	MsG0480021369.01.T01:CDS
CCGTTTACATGCTACTCTAA+GGG	0.503600	4:-57163877	MsG0480021369.01.T01:CDS
ATATAACATCTCGCTTGAAT+GGG	0.506633	4:-57163635	MsG0480021369.01.T01:CDS
GGATATGTTGCAGTGTATGT+AGG	0.540916	4:-57163804	MsG0480021369.01.T01:CDS
ATGTAGGAGAGGAACAGAAG+AGG	0.560493	4:-57163788	MsG0480021369.01.T01:CDS
GACATCTTCTGTGCAAGGAA+TGG	0.561649	4:+57163665	MsG0480021370.01.T01:CDS
AGGAATGGTGAGTCCACCCA+TGG	0.569046	4:+57163680	MsG0480021370.01.T01:CDS
GTTTGGATATGATCATCCCA+TGG	0.570301	4:-57163697	MsG0480021369.01.T01:CDS
CTCAACAAGTCTTGAAACGA+TGG	0.576425	4:+57163735	MsG0480021370.01.T01:CDS
AAATCTGTAGAAGTGAGAAA+GGG	0.578349	4:-57163825	MsG0480021369.01.T01:CDS
TGAAAGACATCTTCTGTGCA+AGG	0.587793	4:+57163660	MsG0480021370.01.T01:CDS
ACAAGTCTTGAAACGATGGT+TGG	0.594658	4:+57163739	MsG0480021370.01.T01:intron
TGTTGCAGTGTATGTAGGAG+AGG	0.608190	4:-57163799	MsG0480021369.01.T01:CDS
AGACTTGTTGAGTCAAGCTG+AGG	0.628331	4:-57163724	MsG0480021369.01.T01:CDS
GGATATGATCATCCCATGGG+TGG	0.636117	4:-57163693	MsG0480021369.01.T01:CDS
TTTGGATATGATCATCCCAT+GGG	0.641263	4:-57163696	MsG0480021369.01.T01:CDS
GGAATGGTGAGTCCACCCAT+GGG	0.658493	4:+57163681	MsG0480021370.01.T01:CDS
GTTGGTTCAAGTATGATATG+GGG	0.673944	4:+57163757	MsG0480021370.01.T01:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAATCTGTAGAAGTGAGAA+AGG	-	Chr4:57163688-57163707	MsG0480021369.01.T01:CDS	30.0%
	AAATCTGTAGAAGTGAGAAA+GGG	-	Chr4:57163689-57163708	MsG0480021369.01.T01:CDS	30.0%
	ATATAACATCTCGCTTGAAT+GGG	-	Chr4:57163879-57163898	MsG0480021369.01.T01:CDS	30.0%
	CATATAACATCTCGCTTGAA+TGG	-	Chr4:57163878-57163897	MsG0480021369.01.T01:CDS	35.0%
	TTTGGATATGATCATCCCAT+GGG	-	Chr4:57163818-57163837	MsG0480021369.01.T01:CDS	35.0%
!	GGTTGGTTCAAGTATGATAT+GGG	+	Chr4:57163761-57163780	MsG0480021370.01.T01:intron	35.0%
!	GTTGGTTCAAGTATGATATG+GGG	+	Chr4:57163760-57163779	MsG0480021370.01.T01:intron	35.0%
!	TGGTTGGTTCAAGTATGATA+TGG	+	Chr4:57163762-57163781	MsG0480021370.01.T01:intron	35.0%
	ACAAGTCTTGAAACGATGGT+TGG	+	Chr4:57163778-57163797	MsG0480021370.01.T01:intron	40.0%
	CCCTTAGAGTAGCATGTAAA+CGG	+	Chr4:57163640-57163659	None:intergenic	40.0%
	CCGTTTACATGCTACTCTAA+GGG	-	Chr4:57163637-57163656	MsG0480021369.01.T01:CDS	40.0%
	CTCAACAAGTCTTGAAACGA+TGG	+	Chr4:57163782-57163801	MsG0480021370.01.T01:intron	40.0%
	GGATATGTTGCAGTGTATGT+AGG	-	Chr4:57163710-57163729	MsG0480021369.01.T01:CDS	40.0%
	GTTTGGATATGATCATCCCA+TGG	-	Chr4:57163817-57163836	MsG0480021369.01.T01:CDS	40.0%
	TCCGTTTACATGCTACTCTA+AGG	-	Chr4:57163636-57163655	MsG0480021369.01.T01:CDS	40.0%
	TGAAAGACATCTTCTGTGCA+AGG	+	Chr4:57163857-57163876	MsG0480021370.01.T01:intron	40.0%
	AGTCAAGCTGAGGAAGAGTT+TGG	-	Chr4:57163800-57163819	MsG0480021369.01.T01:CDS	45.0%
	ATGTAGGAGAGGAACAGAAG+AGG	-	Chr4:57163726-57163745	MsG0480021369.01.T01:CDS	45.0%
	GACATCTTCTGTGCAAGGAA+TGG	+	Chr4:57163852-57163871	MsG0480021370.01.T01:intron	45.0%
	TGTTGCAGTGTATGTAGGAG+AGG	-	Chr4:57163715-57163734	MsG0480021369.01.T01:CDS	45.0%
!!	AGACTTGTTGAGTCAAGCTG+AGG	-	Chr4:57163790-57163809	MsG0480021369.01.T01:CDS	45.0%
	GGATATGATCATCCCATGGG+TGG	-	Chr4:57163821-57163840	MsG0480021369.01.T01:CDS	50.0%
	AGGAATGGTGAGTCCACCCA+TGG	+	Chr4:57163837-57163856	MsG0480021370.01.T01:intron	55.0%
	GGAATGGTGAGTCCACCCAT+GGG	+	Chr4:57163836-57163855	MsG0480021370.01.T01:intron	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	57163629	57163907	57163629	ID=MsG0480021369.01;Name=MsG0480021369.01
Chr4	mRNA	57163629	57163907	57163629	ID=MsG0480021369.01.T01;Parent=MsG0480021369.01;Name=MsG0480021369.01.T01;_AED=0.40;_eAED=0.40;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|92
Chr4	exon	57163629	57163907	57163629	ID=MsG0480021369.01.T01:exon:19226;Parent=MsG0480021369.01.T01
Chr4	CDS	57163629	57163907	57163629	ID=MsG0480021369.01.T01:cds;Parent=MsG0480021369.01.T01

Gene Sequence	>MsG0480021369.01.T01 ATGGGTTTCCGTTTACATGCTACTCTAAGGGCATCAGTAACTGCTAGACAAGCATCATCAAAATCTGTAGAAGTGAGAAAGGGATATGTTGCAGTGTATGTAGGAGAGGAACAGAAGAGGTTTGTGATCCCCATATCATACTTGAACCAACCATCGTTTCAAGACTTGTTGAGTCAAGCTGAGGAAGAGTTTGGATATGATCATCCCATGGGTGGACTCACCATTCCTTGCACAGAAGATGTCTTTCAACATATAACATCTCGCTTGAATGGGCTATAA
Protein sequence	>MsG0480021369.01.T01 MGFRLHATLRASVTARQASSKSVEVRKGYVAVYVGEEQKRFVIPISYLNQPSFQDLLSQAEEEFGYDHPMGGLTIPCTEDVFQHITSRLNGL*