AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020890.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020890.01.T01 MTR_4g062270 100.000 114 0 0 1 114 351 464 3.07e-81 245
MsG0480020890.01.T01 MTR_4g062170 97.368 114 3 0 1 114 359 472 3.42e-79 240
MsG0480020890.01.T01 MTR_4g062190 92.035 113 9 0 2 114 361 473 2.37e-74 228
MsG0480020890.01.T01 MTR_2g096210 77.876 113 21 2 1 109 358 470 1.20e-59 190
MsG0480020890.01.T01 MTR_1g115560 75.510 98 24 0 3 100 371 468 2.15e-51 169
MsG0480020890.01.T01 MTR_4g070270 57.143 84 34 1 2 83 416 499 3.18e-30 112
MsG0480020890.01.T01 MTR_7g118170 60.465 86 29 2 3 83 440 525 1.17e-29 111
MsG0480020890.01.T01 MTR_5g056510 57.143 84 34 1 2 83 404 487 1.19e-29 110
MsG0480020890.01.T01 MTR_3g105550 58.333 84 32 1 3 83 450 533 1.99e-29 110
MsG0480020890.01.T01 MTR_1g110930 57.143 84 34 1 2 83 404 487 3.60e-29 109
MsG0480020890.01.T01 MTR_7g118160 55.814 86 33 1 3 83 331 416 3.42e-28 106
MsG0480020890.01.T01 MTR_1g041085 57.647 85 33 1 2 83 425 509 7.79e-28 106
MsG0480020890.01.T01 MTR_2g436480 54.762 84 36 1 2 83 365 448 1.65e-27 104
MsG0480020890.01.T01 MTR_1g076430 57.143 84 33 1 3 83 420 503 3.61e-27 104
MsG0480020890.01.T01 MTR_6g074880 51.190 84 38 1 3 83 382 465 3.82e-27 103
MsG0480020890.01.T01 MTR_4g054415 51.190 84 38 1 3 83 339 422 4.40e-27 103
MsG0480020890.01.T01 MTR_4g054630 51.190 84 38 1 3 83 380 463 5.82e-27 103
MsG0480020890.01.T01 MTR_2g054940 55.952 84 34 1 3 83 419 502 1.50e-26 102
MsG0480020890.01.T01 MTR_2g007020 56.471 85 33 2 2 83 365 448 9.62e-26 100
MsG0480020890.01.T01 MTR_4g113280 47.475 99 33 1 3 101 397 476 1.04e-25 100
MsG0480020890.01.T01 MTR_7g081695 54.762 84 35 1 3 83 407 490 1.47e-25 100
MsG0480020890.01.T01 MTR_4g053905 50.000 84 39 1 3 83 73 156 2.00e-25 94.0
MsG0480020890.01.T01 MTR_6g032965 46.875 96 48 1 2 94 297 392 3.44e-24 95.1
MsG0480020890.01.T01 MTR_6g051440 50.000 84 39 2 3 83 398 481 1.24e-21 88.6
MsG0480020890.01.T01 MTR_1g102290 50.000 84 39 2 3 83 400 483 4.03e-21 87.0
MsG0480020890.01.T01 MTR_3g072940 48.810 84 40 2 3 83 399 482 4.81e-21 87.0
MsG0480020890.01.T01 MTR_3g072980 48.810 84 40 2 3 83 399 482 4.91e-21 87.0
MsG0480020890.01.T01 MTR_2g013740 56.716 67 29 0 3 69 426 492 1.53e-20 85.5
MsG0480020890.01.T01 MTR_4g054260 39.286 84 31 2 3 83 256 322 1.70e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020890.01.T01 AT1G07720 77.193 114 22 2 2 111 405 518 5.61e-64 202
MsG0480020890.01.T01 AT1G07720 77.193 114 22 2 2 111 362 475 2.89e-61 194
MsG0480020890.01.T01 AT2G28630 71.930 114 27 2 2 111 361 473 1.19e-57 185
MsG0480020890.01.T01 AT2G28630 71.930 114 27 2 2 111 361 473 1.19e-57 185
MsG0480020890.01.T01 AT5G04530 65.000 100 34 1 2 100 356 455 4.39e-44 149
MsG0480020890.01.T01 AT5G49070 58.824 85 33 1 1 83 368 452 8.45e-33 119
MsG0480020890.01.T01 AT1G19440 58.333 84 33 1 2 83 428 511 4.70e-30 112
MsG0480020890.01.T01 AT5G43760 55.814 86 34 1 2 83 432 517 1.10e-29 111
MsG0480020890.01.T01 AT3G10280 56.977 86 32 1 3 83 370 455 3.34e-29 109
MsG0480020890.01.T01 AT4G34510 56.977 86 35 1 2 85 399 484 3.67e-29 109
MsG0480020890.01.T01 AT2G46720 56.977 86 32 1 3 83 377 462 3.83e-29 109
MsG0480020890.01.T01 AT2G16280 56.977 86 35 1 2 85 424 509 6.00e-29 109
MsG0480020890.01.T01 AT1G01120 55.172 87 35 1 3 85 439 525 1.51e-28 108
MsG0480020890.01.T01 AT2G26250 57.143 84 33 1 3 83 464 547 1.88e-28 108
MsG0480020890.01.T01 AT4G34250 53.571 84 37 1 2 83 401 484 3.16e-28 107
MsG0480020890.01.T01 AT2G15090 58.333 84 33 2 2 83 393 476 5.43e-28 106
MsG0480020890.01.T01 AT1G04220 52.809 89 35 1 2 83 424 512 6.23e-28 106
MsG0480020890.01.T01 AT4G34520 54.651 86 37 2 2 85 404 489 1.07e-27 105
MsG0480020890.01.T01 AT1G25450 53.571 84 37 1 2 83 400 483 4.52e-27 103
MsG0480020890.01.T01 AT2G26640 55.294 85 35 1 2 83 416 500 8.64e-27 103
MsG0480020890.01.T01 AT1G68530 55.422 83 35 1 3 83 406 488 9.06e-27 103
MsG0480020890.01.T01 AT1G71160 50.602 83 39 1 3 83 363 445 1.90e-23 93.6
MsG0480020890.01.T01 AT3G52160 55.224 67 30 0 3 69 261 327 1.47e-22 90.1
MsG0480020890.01.T01 AT3G52160 55.224 67 30 0 3 69 374 440 5.45e-22 89.7

Find 24 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAATGCAATAGTTGCTTA+TGG 0.231406 4:+50195947 MsG0480020890.01.T01:CDS
TAATTATCCACCAAAGTCTT+TGG 0.310010 4:+50196020 MsG0480020890.01.T01:CDS
CGATGTAGTGTCATCCTTGC+AGG 0.324865 4:-50195814 None:intergenic
TCAGGAAGTTCATAATTGTT+AGG 0.335455 4:-50196090 None:intergenic
GTCTTTGGTCAATCCTTTCA+TGG 0.341262 4:+50196035 MsG0480020890.01.T01:CDS
AGTAGCTTGTGGTATGTGTT+AGG 0.359473 4:+50195856 MsG0480020890.01.T01:CDS
CAAATGCAATAGTTGCTTAT+GGG 0.367150 4:+50195948 MsG0480020890.01.T01:CDS
CAGCCTCACTTAAGAAAATC+AGG 0.372574 4:-50196108 None:intergenic
AATCCTTTCATGGAGAAGTA+TGG 0.384841 4:+50196045 MsG0480020890.01.T01:CDS
AGGATGACACTACATCGTTT+TGG 0.411848 4:+50195820 MsG0480020890.01.T01:CDS
TGTTGGAGATGCAAATGTTT+GGG 0.448918 4:+50195984 MsG0480020890.01.T01:CDS
GTAGCTTGTGGTATGTGTTA+GGG 0.465874 4:+50195857 MsG0480020890.01.T01:CDS
ATGTTGGAGATGCAAATGTT+TGG 0.481221 4:+50195983 MsG0480020890.01.T01:CDS
TTTCATGGAGAAGTATGGTT+GGG 0.519323 4:+50196050 MsG0480020890.01.T01:CDS
AAAGGATTGACCAAAGACTT+TGG 0.522713 4:-50196030 None:intergenic
TTTATGATAAGCTTTGGTGC+TGG 0.524017 4:+50195922 MsG0480020890.01.T01:CDS
GTGGTATGTGTTAGGGTACA+TGG 0.531641 4:+50195864 MsG0480020890.01.T01:CDS
CAACCATACTTCTCCATGAA+AGG 0.543653 4:-50196048 None:intergenic
TGGGAAGTGATGAAAGATGT+TGG 0.557628 4:+50195967 MsG0480020890.01.T01:CDS
CTTTCATGGAGAAGTATGGT+TGG 0.572414 4:+50196049 MsG0480020890.01.T01:CDS
TGGTTGGGTCAATGAAGTTG+AGG 0.602405 4:+50196065 MsG0480020890.01.T01:CDS
GGATTGACCAAAGACTTTGG+TGG 0.615272 4:-50196027 None:intergenic
ACACTTCTGCTAGTAGCTTG+TGG 0.618863 4:+50195845 MsG0480020890.01.T01:CDS
AATATGATCTTGAGCCTGCA+AGG 0.629665 4:+50195800 MsG0480020890.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAGTTTTTATGATAAGCTT+TGG + Chr4:50195916-50195935 MsG0480020890.01.T01:CDS 25.0%
CAAATGCAATAGTTGCTTAT+GGG + Chr4:50195948-50195967 MsG0480020890.01.T01:CDS 30.0%
GCAAAAAAGAGGCTAAAAAA+AGG + Chr4:50195889-50195908 MsG0480020890.01.T01:CDS 30.0%
TAATTATCCACCAAAGTCTT+TGG + Chr4:50196020-50196039 MsG0480020890.01.T01:CDS 30.0%
TCAAATGCAATAGTTGCTTA+TGG + Chr4:50195947-50195966 MsG0480020890.01.T01:CDS 30.0%
TCAGGAAGTTCATAATTGTT+AGG - Chr4:50196093-50196112 None:intergenic 30.0%
AAAGGATTGACCAAAGACTT+TGG - Chr4:50196033-50196052 None:intergenic 35.0%
AATCCTTTCATGGAGAAGTA+TGG + Chr4:50196045-50196064 MsG0480020890.01.T01:CDS 35.0%
ATGTTGGAGATGCAAATGTT+TGG + Chr4:50195983-50196002 MsG0480020890.01.T01:CDS 35.0%
TGTTGGAGATGCAAATGTTT+GGG + Chr4:50195984-50196003 MsG0480020890.01.T01:CDS 35.0%
! TTTATGATAAGCTTTGGTGC+TGG + Chr4:50195922-50195941 MsG0480020890.01.T01:CDS 35.0%
! TTTCATGGAGAAGTATGGTT+GGG + Chr4:50196050-50196069 MsG0480020890.01.T01:CDS 35.0%
AATATGATCTTGAGCCTGCA+AGG + Chr4:50195800-50195819 MsG0480020890.01.T01:CDS 40.0%
CAACCATACTTCTCCATGAA+AGG - Chr4:50196051-50196070 None:intergenic 40.0%
GGTACATGGAAGCAAAAAAG+AGG + Chr4:50195878-50195897 MsG0480020890.01.T01:CDS 40.0%
GTCTTTGGTCAATCCTTTCA+TGG + Chr4:50196035-50196054 MsG0480020890.01.T01:CDS 40.0%
TGGGAAGTGATGAAAGATGT+TGG + Chr4:50195967-50195986 MsG0480020890.01.T01:CDS 40.0%
! AGGATGACACTACATCGTTT+TGG + Chr4:50195820-50195839 MsG0480020890.01.T01:CDS 40.0%
! AGTAGCTTGTGGTATGTGTT+AGG + Chr4:50195856-50195875 MsG0480020890.01.T01:CDS 40.0%
! CTTTCATGGAGAAGTATGGT+TGG + Chr4:50196049-50196068 MsG0480020890.01.T01:CDS 40.0%
! GTAGCTTGTGGTATGTGTTA+GGG + Chr4:50195857-50195876 MsG0480020890.01.T01:CDS 40.0%
ACACTTCTGCTAGTAGCTTG+TGG + Chr4:50195845-50195864 MsG0480020890.01.T01:CDS 45.0%
GGATTGACCAAAGACTTTGG+TGG - Chr4:50196030-50196049 None:intergenic 45.0%
GTGGTATGTGTTAGGGTACA+TGG + Chr4:50195864-50195883 MsG0480020890.01.T01:CDS 45.0%
TGGTTGGGTCAATGAAGTTG+AGG + Chr4:50196065-50196084 MsG0480020890.01.T01:CDS 45.0%
CGATGTAGTGTCATCCTTGC+AGG - Chr4:50195817-50195836 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 50195781 50196125 50195781 ID=MsG0480020890.01;Name=MsG0480020890.01
Chr4 mRNA 50195781 50196125 50195781 ID=MsG0480020890.01.T01;Parent=MsG0480020890.01;Name=MsG0480020890.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|114
Chr4 exon 50195781 50196125 50195781 ID=MsG0480020890.01.T01:exon:271;Parent=MsG0480020890.01.T01
Chr4 CDS 50195781 50196125 50195781 ID=MsG0480020890.01.T01:cds;Parent=MsG0480020890.01.T01
Gene Sequence

>MsG0480020890.01.T01

ATGAGTTTAGATCTAAGTGAATATGATCTTGAGCCTGCAAGGATGACACTACATCGTTTTGGTAACACTTCTGCTAGTAGCTTGTGGTATGTGTTAGGGTACATGGAAGCAAAAAAGAGGCTAAAAAAAGGTGATAGAGTTTTTATGATAAGCTTTGGTGCTGGATTCAAATGCAATAGTTGCTTATGGGAAGTGATGAAAGATGTTGGAGATGCAAATGTTTGGGAAGATTGTATTGATAATTATCCACCAAAGTCTTTGGTCAATCCTTTCATGGAGAAGTATGGTTGGGTCAATGAAGTTGAGGATCCTAACAATTATGAACTTCCTGATTTTCTTAAGTGA

Protein sequence

>MsG0480020890.01.T01

MSLDLSEYDLEPARMTLHRFGNTSASSLWYVLGYMEAKKRLKKGDRVFMISFGAGFKCNSCLWEVMKDVGDANVWEDCIDNYPPKSLVNPFMEKYGWVNEVEDPNNYELPDFLK*