AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022355.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022355.01.T01 MTR_4g088515 97.312 186 5 0 1 186 83 268 1.22e-129 377
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022355.01.T01 AT4G26415 63.636 110 15 4 20 110 114 217 1.26e-32 119

Find 44 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATGAATATAGTGGAATA+AGG 0.272127 4:-71502146 MsG0480022355.01.T01:CDS
TGGTGTTGTGGCAAAGTTAA+TGG 0.280238 4:-71501890 MsG0480022355.01.T01:CDS
GCCATTTGAGAGGCATGATT+TGG 0.298125 4:-71501770 MsG0480022355.01.T01:CDS
TCCATGTATAGGTCATCTTT+TGG 0.313265 4:-71502030 MsG0480022355.01.T01:CDS
TGGATATTGTATTATGAAAC+CGG 0.367697 4:-71501665 MsG0480022355.01.T01:CDS
GGATGAGTAGTGTTGATTGT+AGG 0.385703 4:-71501813 MsG0480022355.01.T01:CDS
CAATGAATATAGTGGAATAA+GGG 0.392950 4:-71502145 MsG0480022355.01.T01:CDS
AGAACCAAAGCTTGATGAAA+AGG 0.407802 4:-71501965 MsG0480022355.01.T01:CDS
GCCATAGCTTCTAGAGAAAC+CGG 0.424661 4:+71501646 None:intergenic
GGTGTTGTGGCAAAGTTAAT+GGG 0.432813 4:-71501889 MsG0480022355.01.T01:CDS
TGTGGCAAAGTTAATGGGTT+TGG 0.433784 4:-71501884 MsG0480022355.01.T01:CDS
CTCCTTGGTTGCCAACTACT+AGG 0.453422 4:+71501616 None:intergenic
GTTAGAAGAAGAAATAGGTC+TGG 0.472977 4:-71501703 MsG0480022355.01.T01:CDS
TCCAAATCATGCCTCTCAAA+TGG 0.478781 4:+71501769 None:intergenic
CTAATCTAAACATATCTCCT+TGG 0.491234 4:+71501601 None:intergenic
AAAACTAGCAATGGACATGC+AGG 0.491726 4:-71501743 MsG0480022355.01.T01:CDS
CCCAAAAGATGACCTATACA+TGG 0.498041 4:+71502029 None:intergenic
TTCTCCCCAGCAACTGTTGG+TGG 0.503702 4:+71501931 None:intergenic
GTAGGAGACAAAATATAAGA+AGG 0.511223 4:-71501795 MsG0480022355.01.T01:CDS
ATGGAAGAGATTCCATGTAT+AGG 0.512387 4:-71502041 MsG0480022355.01.T01:CDS
ATTAACTTTGCCACAACACC+AGG 0.513967 4:+71501892 None:intergenic
TGGCAGGAGGTCCTAGTAGT+TGG 0.521625 4:-71501627 MsG0480022355.01.T01:CDS
ACCGGTTTCTCTAGAAGCTA+TGG 0.522607 4:-71501647 MsG0480022355.01.T01:CDS
TTGCTGGGGAGAACGTGGTT+TGG 0.527912 4:-71501921 MsG0480022355.01.T01:CDS
ACATTGTTAGAAGAAGAAAT+AGG 0.529346 4:-71501708 MsG0480022355.01.T01:CDS
ATGCTTTGAATCAATATCCA+AGG 0.531501 4:-71502074 MsG0480022355.01.T01:CDS
AAATGGAGGAAGAGATGGCA+AGG 0.533678 4:-71502179 MsG0480022355.01.T01:CDS
ATTAGAAATGGAGGAAGAGA+TGG 0.541356 4:-71502184 None:intergenic
CTTCCATCAAGTGAAAACCT+TGG 0.575355 4:+71502057 None:intergenic
GTCCTAGTAGTTGGCAACCA+AGG 0.577078 4:-71501618 MsG0480022355.01.T01:CDS
AATGAATATAGTGGAATAAG+GGG 0.579962 4:-71502144 MsG0480022355.01.T01:CDS
GAAGCTATTCCAGTGTCAGT+AGG 0.605402 4:-71501862 MsG0480022355.01.T01:CDS
TTTGGAGAGAAAACTAGCAA+TGG 0.605568 4:-71501752 MsG0480022355.01.T01:CDS
AACGTGGTTTGGTGTAAACC+TGG 0.608504 4:-71501910 MsG0480022355.01.T01:CDS
GTTTCTCTAGAAGCTATGGC+AGG 0.610709 4:-71501643 MsG0480022355.01.T01:CDS
AACAGTTGCTGGGGAGAACG+TGG 0.612915 4:-71501926 MsG0480022355.01.T01:CDS
AATATAGTGGAATAAGGGGA+AGG 0.620558 4:-71502140 MsG0480022355.01.T01:CDS
GGAGACAAAATATAAGAAGG+AGG 0.626425 4:-71501792 MsG0480022355.01.T01:CDS
TAAGAAGGAGGCCATTTGAG+AGG 0.627512 4:-71501780 MsG0480022355.01.T01:CDS
CTGACATATTAAGATCTGCA+AGG 0.639107 4:-71502098 MsG0480022355.01.T01:CDS
ACGTTCTCCCCAGCAACTGT+TGG 0.657823 4:+71501928 None:intergenic
TCTCTAGAAGCTATGGCAGG+AGG 0.665560 4:-71501640 MsG0480022355.01.T01:CDS
TTGGTGTAAACCTGGTGTTG+TGG 0.669247 4:-71501902 MsG0480022355.01.T01:CDS
AAACTAGCAATGGACATGCA+GGG 0.676573 4:-71501742 MsG0480022355.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACATTGTTAGAAGAAGAAAT+AGG - Chr4:71502075-71502094 MsG0480022355.01.T01:CDS 25.0%
! GAAAAACTCAATGAATATAG+TGG - Chr4:71501630-71501649 MsG0480022355.01.T01:CDS 25.0%
! TGGATATTGTATTATGAAAC+CGG - Chr4:71502118-71502137 MsG0480022355.01.T01:CDS 25.0%
!! AATGAATATAGTGGAATAAG+GGG - Chr4:71501639-71501658 MsG0480022355.01.T01:CDS 25.0%
!! CAATGAATATAGTGGAATAA+GGG - Chr4:71501638-71501657 MsG0480022355.01.T01:CDS 25.0%
!! TCAATGAATATAGTGGAATA+AGG - Chr4:71501637-71501656 MsG0480022355.01.T01:CDS 25.0%
AAAGATCTGACAAAAAAGAG+AGG - Chr4:71501949-71501968 MsG0480022355.01.T01:CDS 30.0%
GTAGGAGACAAAATATAAGA+AGG - Chr4:71501988-71502007 MsG0480022355.01.T01:CDS 30.0%
! ATAACCTTTTCATCAAGCTT+TGG + Chr4:71501825-71501844 None:intergenic 30.0%
! ATGCTTTGAATCAATATCCA+AGG - Chr4:71501709-71501728 MsG0480022355.01.T01:CDS 30.0%
!!! TCTGGTTTTTGCTCTAAAAA+TGG - Chr4:71502098-71502117 MsG0480022355.01.T01:CDS 30.0%
AGAACCAAAGCTTGATGAAA+AGG - Chr4:71501818-71501837 MsG0480022355.01.T01:CDS 35.0%
ATGGAAGAGATTCCATGTAT+AGG - Chr4:71501742-71501761 MsG0480022355.01.T01:CDS 35.0%
CTGACATATTAAGATCTGCA+AGG - Chr4:71501685-71501704 MsG0480022355.01.T01:CDS 35.0%
GGAGACAAAATATAAGAAGG+AGG - Chr4:71501991-71502010 MsG0480022355.01.T01:CDS 35.0%
GTTAGAAGAAGAAATAGGTC+TGG - Chr4:71502080-71502099 MsG0480022355.01.T01:CDS 35.0%
TTTGGAGAGAAAACTAGCAA+TGG - Chr4:71502031-71502050 MsG0480022355.01.T01:CDS 35.0%
! AATATAGTGGAATAAGGGGA+AGG - Chr4:71501643-71501662 MsG0480022355.01.T01:CDS 35.0%
! CCATGTATAGGTCATCTTTT+GGG - Chr4:71501754-71501773 MsG0480022355.01.T01:CDS 35.0%
! TATCCAAGGTTTTCACTTGA+TGG - Chr4:71501723-71501742 MsG0480022355.01.T01:CDS 35.0%
! TCCATGTATAGGTCATCTTT+TGG - Chr4:71501753-71501772 MsG0480022355.01.T01:CDS 35.0%
!!! TCATCTTTTGGGACTATAGA+AGG - Chr4:71501765-71501784 MsG0480022355.01.T01:CDS 35.0%
AAAACTAGCAATGGACATGC+AGG - Chr4:71502040-71502059 MsG0480022355.01.T01:CDS 40.0%
AAACTAGCAATGGACATGCA+GGG - Chr4:71502041-71502060 MsG0480022355.01.T01:CDS 40.0%
ATTAACTTTGCCACAACACC+AGG + Chr4:71501894-71501913 None:intergenic 40.0%
CCCAAAAGATGACCTATACA+TGG + Chr4:71501757-71501776 None:intergenic 40.0%
CTTCCATCAAGTGAAAACCT+TGG + Chr4:71501729-71501748 None:intergenic 40.0%
TCCAAATCATGCCTCTCAAA+TGG + Chr4:71502017-71502036 None:intergenic 40.0%
! CTTTTTCTACCTACTGACAC+TGG + Chr4:71501933-71501952 None:intergenic 40.0%
! GGTGTTGTGGCAAAGTTAAT+GGG - Chr4:71501894-71501913 MsG0480022355.01.T01:CDS 40.0%
!! GGATGAGTAGTGTTGATTGT+AGG - Chr4:71501970-71501989 MsG0480022355.01.T01:CDS 40.0%
!! TGGTGTTGTGGCAAAGTTAA+TGG - Chr4:71501893-71501912 MsG0480022355.01.T01:CDS 40.0%
!! TGTGGCAAAGTTAATGGGTT+TGG - Chr4:71501899-71501918 MsG0480022355.01.T01:CDS 40.0%
GAAGCTATTCCAGTGTCAGT+AGG - Chr4:71501921-71501940 MsG0480022355.01.T01:CDS 45.0%
TAAGAAGGAGGCCATTTGAG+AGG - Chr4:71502003-71502022 MsG0480022355.01.T01:CDS 45.0%
! ACCGGTTTCTCTAGAAGCTA+TGG - Chr4:71502136-71502155 MsG0480022355.01.T01:CDS 45.0%
! GCCATAGCTTCTAGAGAAAC+CGG + Chr4:71502140-71502159 None:intergenic 45.0%
! GCCATTTGAGAGGCATGATT+TGG - Chr4:71502013-71502032 MsG0480022355.01.T01:CDS 45.0%
! GTTTCTCTAGAAGCTATGGC+AGG - Chr4:71502140-71502159 MsG0480022355.01.T01:CDS 45.0%
! GTTTTCCACCAACAGTTGCT+GGG - Chr4:71501847-71501866 MsG0480022355.01.T01:CDS 45.0%
! TGTTTTCCACCAACAGTTGC+TGG - Chr4:71501846-71501865 MsG0480022355.01.T01:CDS 45.0%
! TTGGTGTAAACCTGGTGTTG+TGG - Chr4:71501881-71501900 MsG0480022355.01.T01:CDS 45.0%
! TTTTCCACCAACAGTTGCTG+GGG - Chr4:71501848-71501867 MsG0480022355.01.T01:CDS 45.0%
!! AACGTGGTTTGGTGTAAACC+TGG - Chr4:71501873-71501892 MsG0480022355.01.T01:CDS 45.0%
CTCCTTGGTTGCCAACTACT+AGG + Chr4:71502170-71502189 None:intergenic 50.0%
GTCCTAGTAGTTGGCAACCA+AGG - Chr4:71502165-71502184 MsG0480022355.01.T01:CDS 50.0%
! TCTCTAGAAGCTATGGCAGG+AGG - Chr4:71502143-71502162 MsG0480022355.01.T01:CDS 50.0%
AACAGTTGCTGGGGAGAACG+TGG - Chr4:71501857-71501876 MsG0480022355.01.T01:CDS 55.0%
ACGTTCTCCCCAGCAACTGT+TGG + Chr4:71501858-71501877 None:intergenic 55.0%
TGGCAGGAGGTCCTAGTAGT+TGG - Chr4:71502156-71502175 MsG0480022355.01.T01:CDS 55.0%
TTCTCCCCAGCAACTGTTGG+TGG + Chr4:71501855-71501874 None:intergenic 55.0%
TTGCTGGGGAGAACGTGGTT+TGG - Chr4:71501862-71501881 MsG0480022355.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 71501606 71502199 71501606 ID=MsG0480022355.01;Name=MsG0480022355.01
Chr4 mRNA 71501606 71502199 71501606 ID=MsG0480022355.01.T01;Parent=MsG0480022355.01;Name=MsG0480022355.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|197
Chr4 exon 71501606 71502199 71501606 ID=MsG0480022355.01.T01:exon:19581;Parent=MsG0480022355.01.T01
Chr4 CDS 71501606 71502199 71501606 ID=MsG0480022355.01.T01:cds;Parent=MsG0480022355.01.T01
Gene Sequence

>MsG0480022355.01.T01

ATGGAGGAAGAGATGGCAAGGAAAGAAAAACTCAATGAATATAGTGGAATAAGGGGAAGGATGTCATGTGTTAACAACTCTGACATATTAAGATCTGCAAGGAATGCTTTGAATCAATATCCAAGGTTTTCACTTGATGGAAGAGATTCCATGTATAGGTCATCTTTTGGGACTATAGAAGGAAGAAGATCAGTTTGTAGTGAGATAAGTTTAGAACCAAAGCTTGATGAAAAGGTTATGTGTTTTCCACCAACAGTTGCTGGGGAGAACGTGGTTTGGTGTAAACCTGGTGTTGTGGCAAAGTTAATGGGTTTGGAAGCTATTCCAGTGTCAGTAGGTAGAAAAAGATCTGACAAAAAAGAGAGGATGAGTAGTGTTGATTGTAGGAGACAAAATATAAGAAGGAGGCCATTTGAGAGGCATGATTTGGAGAGAAAACTAGCAATGGACATGCAGGGTTATCGTGATCACATTGTTAGAAGAAGAAATAGGTCTGGTTTTTGCTCTAAAAATGGATATTGTATTATGAAACCGGTTTCTCTAGAAGCTATGGCAGGAGGTCCTAGTAGTTGGCAACCAAGGAGATATGTTTAG

Protein sequence

>MsG0480022355.01.T01

MEEEMARKEKLNEYSGIRGRMSCVNNSDILRSARNALNQYPRFSLDGRDSMYRSSFGTIEGRRSVCSEISLEPKLDEKVMCFPPTVAGENVVWCKPGVVAKLMGLEAIPVSVGRKRSDKKERMSSVDCRRQNIRRRPFERHDLERKLAMDMQGYRDHIVRRRNRSGFCSKNGYCIMKPVSLEAMAGGPSSWQPRRYV*