AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036979.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036979.01.T01 MTR_7g024780 94.318 176 10 0 1 176 1 176 3.71e-125 349
MsG0780036979.01.T01 MTR_7g024765 68.750 176 54 1 1 176 1 175 1.95e-88 256
MsG0780036979.01.T01 MTR_7g024760 68.786 173 53 1 1 173 1 172 2.60e-84 246
MsG0780036979.01.T01 MTR_7g114410 61.582 177 67 1 1 176 1 177 1.41e-77 229
MsG0780036979.01.T01 MTR_7g114410 61.392 158 60 1 1 157 1 158 2.71e-68 205
MsG0780036979.01.T01 MTR_3g463610 50.292 171 81 2 4 170 3 173 5.31e-56 176
MsG0780036979.01.T01 MTR_1g103150 42.105 171 97 2 2 170 17 187 3.05e-43 142
MsG0780036979.01.T01 MTR_1g103110 42.857 168 94 2 2 167 17 184 4.92e-43 142
MsG0780036979.01.T01 MTR_5g006510 44.509 173 89 4 3 170 18 188 1.84e-42 140
MsG0780036979.01.T01 MTR_5g006470 42.353 170 96 2 3 170 16 185 1.32e-41 138
MsG0780036979.01.T01 MTR_5g006450 41.520 171 96 2 4 170 17 187 3.11e-40 135
MsG0780036979.01.T01 MTR_5g006460 44.318 176 88 7 3 170 17 190 1.04e-39 133
MsG0780036979.01.T01 MTR_5g006520 40.805 174 93 3 4 170 1735 1905 3.41e-36 133
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036979.01.T01 AT3G22560 45.614 171 90 2 3 170 2 172 2.43e-47 152
MsG0780036979.01.T01 AT2G32020 41.954 174 96 3 1 172 12 182 5.59e-46 149
MsG0780036979.01.T01 AT2G32030 40.237 169 96 3 6 172 22 187 1.95e-44 145

Find 41 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCTATTGGATTCAACTAT+TGG 0.220333 7:-17470402 MsG0780036979.01.T01:CDS
TGCTATTGGATTCAACTATT+GGG 0.279552 7:-17470401 MsG0780036979.01.T01:CDS
TGAGAAGTATAAAGCAGATA+TGG 0.323753 7:-17470428 MsG0780036979.01.T01:CDS
ACCACTCCATTGGAATGGTT+TGG 0.355259 7:-17470471 MsG0780036979.01.T01:CDS
GCAGATATGGGTTATGCTAT+TGG 0.359042 7:-17470415 MsG0780036979.01.T01:CDS
AGCTTGCAACCTTCTCAAAT+CGG 0.366599 7:+17470318 None:intergenic
TAATTCAAAGAAAGAAGCTT+TGG 0.384761 7:-17470548 MsG0780036979.01.T01:CDS
CATAGCTTCTCAGAGGGTAT+TGG 0.423997 7:-17470278 MsG0780036979.01.T01:CDS
GCTTGCAACCTTCTCAAATC+GGG 0.427428 7:+17470319 None:intergenic
ACGTAGATGATGTGTTATTA+TGG 0.435820 7:-17470615 MsG0780036979.01.T01:CDS
TCTACGTCACTTTCCTTGAA+TGG 0.443643 7:+17470631 None:intergenic
AAGAATCTTCACTGCATTAG+TGG 0.453856 7:+17470366 None:intergenic
GGTCGAATAGTGATCCTTGA+TGG 0.461144 7:+17470652 None:intergenic
CAAAGAGGGTTTGCTCAGAA+AGG 0.466394 7:-17470239 MsG0780036979.01.T01:CDS
TTCTCAAATCGGGGAACTCA+TGG 0.488747 7:+17470329 None:intergenic
CAGAGGGTATTGGAAAAGCT+AGG 0.497928 7:-17470268 MsG0780036979.01.T01:CDS
GGTCATCAAGACATATTGAT+TGG 0.500106 7:+17470491 None:intergenic
GAGAAGTATAAAGCAGATAT+GGG 0.502094 7:-17470427 MsG0780036979.01.T01:CDS
TGTCTTGATGACCACTCCAT+TGG 0.510495 7:-17470481 MsG0780036979.01.T01:CDS
GATCACTATTCGACCATTCA+AGG 0.514889 7:-17470644 MsG0780036979.01.T01:CDS
GGATTCAACTATTGGGGTCA+AGG 0.521820 7:-17470394 MsG0780036979.01.T01:CDS
TGATGACCACTCCATTGGAA+TGG 0.535636 7:-17470476 MsG0780036979.01.T01:CDS
AGACATATTGATTGGCGTAA+GGG 0.538608 7:+17470499 None:intergenic
GCTATTGGATTCAACTATTG+GGG 0.539184 7:-17470400 MsG0780036979.01.T01:CDS
GACATATTGATTGGCGTAAG+GGG 0.541235 7:+17470500 None:intergenic
AAGACATATTGATTGGCGTA+AGG 0.551674 7:+17470498 None:intergenic
ATGAGTTCCCCGATTTGAGA+AGG 0.555146 7:-17470327 MsG0780036979.01.T01:CDS
TGGCAATAATAAATCCATCA+AGG 0.562148 7:-17470666 MsG0780036979.01.T01:CDS
AATACTAACTAAACAACAGA+AGG 0.576587 7:+17470151 None:intergenic
AAGCTAGGGTTCTACAAAGA+GGG 0.577948 7:-17470253 MsG0780036979.01.T01:CDS
AAGAATCCAAACCATTCCAA+TGG 0.587139 7:+17470465 None:intergenic
AGTAACTGAGCATACTCGAT+GGG 0.588466 7:-17470578 MsG0780036979.01.T01:CDS
AGAGGGTATTGGAAAAGCTA+GGG 0.597985 7:-17470267 MsG0780036979.01.T01:CDS
GATGATGTGTTATTATGGCT+AGG 0.599337 7:-17470610 MsG0780036979.01.T01:CDS
TACTTCTCATCATTAGCATG+AGG 0.611961 7:+17470442 None:intergenic
TCCAAACCATTCCAATGGAG+TGG 0.617327 7:+17470470 None:intergenic
GAGTAACTGAGCATACTCGA+TGG 0.628723 7:-17470579 MsG0780036979.01.T01:CDS
AAAGCTAGGGTTCTACAAAG+AGG 0.630805 7:-17470254 MsG0780036979.01.T01:CDS
CTTGCAACCTTCTCAAATCG+GGG 0.653528 7:+17470320 None:intergenic
TAGAGAACATAGCTTCTCAG+AGG 0.672958 7:-17470285 MsG0780036979.01.T01:CDS
AGAGAACATAGCTTCTCAGA+GGG 0.687021 7:-17470284 MsG0780036979.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGAAAGGTTTTTTATTTTAA+AGG - Chr7:17470603-17470622 MsG0780036979.01.T01:CDS 15.0%
!!! TCTATATTTTTAGCTTTCTT+TGG - Chr7:17470640-17470659 MsG0780036979.01.T01:CDS 20.0%
!!! TTATTTTAAAGGAAACTTTG+TGG - Chr7:17470614-17470633 MsG0780036979.01.T01:CDS 20.0%
! TAATTCAAAGAAAGAAGCTT+TGG - Chr7:17470278-17470297 MsG0780036979.01.T01:CDS 25.0%
ATGCTATTGGATTCAACTAT+TGG - Chr7:17470424-17470443 MsG0780036979.01.T01:CDS 30.0%
TGCTATTGGATTCAACTATT+GGG - Chr7:17470425-17470444 MsG0780036979.01.T01:CDS 30.0%
TGGCAATAATAAATCCATCA+AGG - Chr7:17470160-17470179 MsG0780036979.01.T01:CDS 30.0%
! ACGTAGATGATGTGTTATTA+TGG - Chr7:17470211-17470230 MsG0780036979.01.T01:CDS 30.0%
! GAGAAGTATAAAGCAGATAT+GGG - Chr7:17470399-17470418 MsG0780036979.01.T01:CDS 30.0%
! TGAGAAGTATAAAGCAGATA+TGG - Chr7:17470398-17470417 MsG0780036979.01.T01:CDS 30.0%
AAGAATCCAAACCATTCCAA+TGG + Chr7:17470364-17470383 None:intergenic 35.0%
AAGAATCTTCACTGCATTAG+TGG + Chr7:17470463-17470482 None:intergenic 35.0%
GCTATTGGATTCAACTATTG+GGG - Chr7:17470426-17470445 MsG0780036979.01.T01:CDS 35.0%
GGTCATCAAGACATATTGAT+TGG + Chr7:17470338-17470357 None:intergenic 35.0%
TACTTCTCATCATTAGCATG+AGG + Chr7:17470387-17470406 None:intergenic 35.0%
! AAGACATATTGATTGGCGTA+AGG + Chr7:17470331-17470350 None:intergenic 35.0%
! AGACATATTGATTGGCGTAA+GGG + Chr7:17470330-17470349 None:intergenic 35.0%
! GATGATGTGTTATTATGGCT+AGG - Chr7:17470216-17470235 MsG0780036979.01.T01:CDS 35.0%
AAAGCTAGGGTTCTACAAAG+AGG - Chr7:17470572-17470591 MsG0780036979.01.T01:CDS 40.0%
AAGCTAGGGTTCTACAAAGA+GGG - Chr7:17470573-17470592 MsG0780036979.01.T01:CDS 40.0%
AGAGAACATAGCTTCTCAGA+GGG - Chr7:17470542-17470561 MsG0780036979.01.T01:CDS 40.0%
AGCTTGCAACCTTCTCAAAT+CGG + Chr7:17470511-17470530 None:intergenic 40.0%
AGTAACTGAGCATACTCGAT+GGG - Chr7:17470248-17470267 MsG0780036979.01.T01:CDS 40.0%
GATCACTATTCGACCATTCA+AGG - Chr7:17470182-17470201 MsG0780036979.01.T01:CDS 40.0%
TAGAGAACATAGCTTCTCAG+AGG - Chr7:17470541-17470560 MsG0780036979.01.T01:CDS 40.0%
! GACATATTGATTGGCGTAAG+GGG + Chr7:17470329-17470348 None:intergenic 40.0%
! GCAGATATGGGTTATGCTAT+TGG - Chr7:17470411-17470430 MsG0780036979.01.T01:CDS 40.0%
! TCTACGTCACTTTCCTTGAA+TGG + Chr7:17470198-17470217 None:intergenic 40.0%
!! AGAGGGTATTGGAAAAGCTA+GGG - Chr7:17470559-17470578 MsG0780036979.01.T01:CDS 40.0%
ACCACTCCATTGGAATGGTT+TGG - Chr7:17470355-17470374 MsG0780036979.01.T01:CDS 45.0%
CTTGCAACCTTCTCAAATCG+GGG + Chr7:17470509-17470528 None:intergenic 45.0%
GAGTAACTGAGCATACTCGA+TGG - Chr7:17470247-17470266 MsG0780036979.01.T01:CDS 45.0%
GCTTGCAACCTTCTCAAATC+GGG + Chr7:17470510-17470529 None:intergenic 45.0%
GGATTCAACTATTGGGGTCA+AGG - Chr7:17470432-17470451 MsG0780036979.01.T01:CDS 45.0%
GGTCGAATAGTGATCCTTGA+TGG + Chr7:17470177-17470196 None:intergenic 45.0%
TCCAAACCATTCCAATGGAG+TGG + Chr7:17470359-17470378 None:intergenic 45.0%
TGATGACCACTCCATTGGAA+TGG - Chr7:17470350-17470369 MsG0780036979.01.T01:CDS 45.0%
TTCTCAAATCGGGGAACTCA+TGG + Chr7:17470500-17470519 None:intergenic 45.0%
! ATGAGTTCCCCGATTTGAGA+AGG - Chr7:17470499-17470518 MsG0780036979.01.T01:CDS 45.0%
! CAAAGAGGGTTTGCTCAGAA+AGG - Chr7:17470587-17470606 MsG0780036979.01.T01:CDS 45.0%
! CATAGCTTCTCAGAGGGTAT+TGG - Chr7:17470548-17470567 MsG0780036979.01.T01:CDS 45.0%
! TGTCTTGATGACCACTCCAT+TGG - Chr7:17470345-17470364 MsG0780036979.01.T01:CDS 45.0%
!! CAGAGGGTATTGGAAAAGCT+AGG - Chr7:17470558-17470577 MsG0780036979.01.T01:CDS 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 17470159 17470689 17470159 ID=MsG0780036979.01;Name=MsG0780036979.01
Chr7 mRNA 17470159 17470689 17470159 ID=MsG0780036979.01.T01;Parent=MsG0780036979.01;Name=MsG0780036979.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|176
Chr7 exon 17470159 17470689 17470159 ID=MsG0780036979.01.T01:exon:3262;Parent=MsG0780036979.01.T01
Chr7 CDS 17470159 17470689 17470159 ID=MsG0780036979.01.T01:cds;Parent=MsG0780036979.01.T01
Gene Sequence

>MsG0780036979.01.T01

ATGGCAATAATAAATCCATCAAGGATCACTATTCGACCATTCAAGGAAAGTGACGTAGATGATGTGTTATTATGGCTAGGAGATGAAAGAGTAACTGAGCATACTCGATGGGAAACTTGTAATTCAAAGAAAGAAGCTTTGGATTTCATAAAAAATGAATGCATTTACCCCTTACGCCAATCAATATGTCTTGATGACCACTCCATTGGAATGGTTTGGATTCTTCCTCATGCTAATGATGAGAAGTATAAAGCAGATATGGGTTATGCTATTGGATTCAACTATTGGGGTCAAGGAATTGCCACTAATGCAGTGAAGATTCTTCTGTCAAAAGTGTTCCATGAGTTCCCCGATTTGAGAAGGTTGCAAGCTTATACTGTTCTAGAGAACATAGCTTCTCAGAGGGTATTGGAAAAGCTAGGGTTCTACAAAGAGGGTTTGCTCAGAAAGGTTTTTTATTTTAAAGGAAACTTTGTGGATTTCTATATTTTTAGCTTTCTTTGGACAGATGAAATTCCTTCTGTTGTTTAG

Protein sequence

>MsG0780036979.01.T01

MAIINPSRITIRPFKESDVDDVLLWLGDERVTEHTRWETCNSKKEALDFIKNECIYPLRQSICLDDHSIGMVWILPHANDEKYKADMGYAIGFNYWGQGIATNAVKILLSKVFHEFPDLRRLQAYTVLENIASQRVLEKLGFYKEGLLRKVFYFKGNFVDFYIFSFLWTDEIPSVV*