AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021961.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021961.01.T01 MTR_4g079770 96.232 345 12 1 1 344 1 345 0.0 693
MsG0480021961.01.T01 MTR_4g079470 95.942 345 13 1 1 344 1 345 0.0 662
MsG0480021961.01.T01 MTR_5g022560 89.855 345 33 2 1 344 1 344 0.0 645
MsG0480021961.01.T01 MTR_4g107930 89.275 345 36 1 1 344 1 345 0.0 644
MsG0480021961.01.T01 MTR_4g079800 91.594 345 28 1 1 344 1 345 0.0 634
MsG0480021961.01.T01 MTR_4g047610 89.565 345 33 3 1 344 1 343 0.0 623
MsG0480021961.01.T01 MTR_2g075830 77.101 345 74 4 1 344 1 341 0.0 530
MsG0480021961.01.T01 MTR_4g080700 65.839 322 101 8 26 344 25 340 7.07e-151 428
MsG0480021961.01.T01 MTR_4g080730 65.528 322 102 7 26 344 25 340 4.29e-150 426
MsG0480021961.01.T01 MTR_4g080360 53.824 340 151 5 8 344 6 342 1.68e-130 376
MsG0480021961.01.T01 MTR_4g104370 57.396 338 134 8 10 342 10 342 8.76e-130 375
MsG0480021961.01.T01 MTR_2g083930 52.149 349 153 5 1 342 1 342 1.50e-127 369
MsG0480021961.01.T01 MTR_3g075390 54.303 337 145 6 11 342 11 343 7.67e-126 365
MsG0480021961.01.T01 MTR_4g079440 55.484 310 131 4 34 342 40 343 2.81e-122 355
MsG0480021961.01.T01 MTR_3g116080 52.035 344 151 7 7 343 11 347 1.18e-120 352
MsG0480021961.01.T01 MTR_7g078600 57.742 310 119 7 35 342 37 336 8.97e-119 347
MsG0480021961.01.T01 MTR_1g107550 54.921 315 134 5 33 342 43 354 2.41e-118 350
MsG0480021961.01.T01 MTR_7g079230 52.187 343 154 7 1 342 1 334 4.08e-118 345
MsG0480021961.01.T01 MTR_2g090890 49.854 343 159 5 7 343 1 336 7.49e-118 344
MsG0480021961.01.T01 MTR_4g094918 48.387 341 165 7 10 343 5 341 8.34e-114 335
MsG0480021961.01.T01 MTR_1g018840 47.945 365 157 8 4 342 3 360 8.68e-113 335
MsG0480021961.01.T01 MTR_1g018840 47.945 365 157 8 4 342 3 360 1.06e-112 336
MsG0480021961.01.T01 MTR_3g102220 47.684 367 160 10 1 342 1 360 5.47e-111 332
MsG0480021961.01.T01 MTR_3g112430 50.161 311 141 5 41 343 37 341 2.40e-102 308
MsG0480021961.01.T01 MTR_2g015850 54.828 290 123 6 56 342 78 362 3.66e-99 297
MsG0480021961.01.T01 MTR_2g016180 43.931 346 172 10 12 343 18 355 6.52e-89 275
MsG0480021961.01.T01 MTR_2g016270 44.444 342 161 11 12 343 18 340 4.02e-87 270
MsG0480021961.01.T01 MTR_2g067900 44.688 320 154 12 39 343 15 326 8.84e-78 241
MsG0480021961.01.T01 MTR_2g067560 44.860 321 145 13 39 338 15 324 5.96e-73 243
MsG0480021961.01.T01 MTR_4g125300 41.234 308 163 10 42 343 53 348 1.58e-69 221
MsG0480021961.01.T01 MTR_2g064475 39.714 350 153 16 39 343 15 351 1.98e-69 221
MsG0480021961.01.T01 MTR_2g015920 39.308 318 179 10 34 343 47 358 6.42e-68 218
MsG0480021961.01.T01 MTR_4g065077 38.318 321 180 9 33 343 44 356 4.27e-67 215
MsG0480021961.01.T01 MTR_7g078630 45.105 286 116 5 29 303 695 950 2.48e-66 225
MsG0480021961.01.T01 MTR_2g066980 34.930 355 169 12 11 343 4 318 1.53e-65 210
MsG0480021961.01.T01 MTR_4g071060 39.490 314 164 10 38 335 45 348 2.26e-65 211
MsG0480021961.01.T01 MTR_1g023210 37.982 337 167 12 24 335 33 352 4.52e-63 205
MsG0480021961.01.T01 MTR_8g086470 37.304 319 170 9 33 343 60 356 3.53e-62 202
MsG0480021961.01.T01 MTR_1g023810 45.022 231 118 6 114 341 37 261 1.29e-61 198
MsG0480021961.01.T01 MTR_2g015930 39.252 321 168 12 38 343 50 358 2.03e-61 201
MsG0480021961.01.T01 MTR_1g094135 36.202 337 177 12 24 335 35 358 4.11e-57 190
MsG0480021961.01.T01 MTR_4g072790 34.343 297 158 8 42 335 50 312 6.74e-45 156
MsG0480021961.01.T01 MTR_2g015840 50.000 158 74 4 7 161 1 156 3.31e-43 150
MsG0480021961.01.T01 MTR_2g015840 55.357 112 49 1 232 343 161 271 5.43e-36 132
MsG0480021961.01.T01 MTR_4g072340 34.219 301 157 10 42 335 50 316 5.67e-42 149
MsG0480021961.01.T01 MTR_4g073660 33.887 301 158 10 42 335 49 315 6.62e-42 149
MsG0480021961.01.T01 MTR_7g078580 64.286 98 34 1 245 342 4 100 1.61e-41 141
MsG0480021961.01.T01 MTR_7g079130 70.000 90 27 0 253 342 7 96 9.65e-41 138
MsG0480021961.01.T01 MTR_1g025450 38.503 187 90 7 114 296 43 208 1.43e-32 127
MsG0480021961.01.T01 MTR_7g078310 45.000 140 67 3 83 219 115 247 2.44e-27 108
MsG0480021961.01.T01 MTR_2g016380 47.107 121 57 4 227 343 18 135 5.53e-27 107
MsG0480021961.01.T01 MTR_7g079260 61.111 72 12 1 269 340 7 62 9.52e-23 91.7
MsG0480021961.01.T01 MTR_7g111060 28.889 225 132 7 132 335 113 330 1.48e-22 97.4
MsG0480021961.01.T01 MTR_7g111050 29.787 235 135 8 132 343 110 337 5.71e-22 95.5
MsG0480021961.01.T01 MTR_3g029560 27.888 251 146 9 99 343 16 237 6.13e-21 90.5
MsG0480021961.01.T01 MTR_8g087510 41.985 131 69 5 167 295 23 148 7.48e-21 88.6
MsG0480021961.01.T01 MTR_2g047960 48.235 85 43 1 135 219 7 90 8.25e-18 78.2
MsG0480021961.01.T01 MTR_3g082370 26.087 207 133 5 131 322 5 206 2.53e-17 80.5
MsG0480021961.01.T01 MTR_7g111050 27.692 195 117 6 132 305 105 296 1.14e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021961.01.T01 AT5G45890 55.652 345 143 5 7 343 3 345 7.69e-136 390
MsG0480021961.01.T01 AT5G50260 54.783 345 131 7 15 342 5 341 9.38e-130 375
MsG0480021961.01.T01 AT3G48340 54.070 344 139 7 13 342 4 342 3.45e-123 358
MsG0480021961.01.T01 AT2G34080 50.585 342 157 6 11 344 8 345 1.15e-120 352
MsG0480021961.01.T01 AT4G35350 50.872 344 152 8 10 343 16 352 1.02e-117 344
MsG0480021961.01.T01 AT3G19390 50.146 343 160 6 9 343 6 345 9.69e-117 345
MsG0480021961.01.T01 AT5G43060 53.681 326 136 7 25 342 34 352 8.02e-116 343
MsG0480021961.01.T01 AT3G48350 54.890 317 133 7 33 343 31 343 1.79e-115 339
MsG0480021961.01.T01 AT3G49340 50.442 339 148 7 21 344 8 341 1.71e-114 336
MsG0480021961.01.T01 AT2G27420 48.571 350 160 7 10 344 4 348 1.72e-114 336
MsG0480021961.01.T01 AT1G06260 51.884 345 146 7 8 342 7 341 8.65e-113 332
MsG0480021961.01.T01 AT1G20850 52.077 313 143 5 34 343 45 353 4.42e-111 328
MsG0480021961.01.T01 AT4G36880 50.467 321 146 6 33 342 42 360 8.58e-111 328
MsG0480021961.01.T01 AT3G19400 50.814 307 146 5 40 343 44 348 2.42e-110 326
MsG0480021961.01.T01 AT1G29090 46.176 353 174 7 4 344 7 355 2.76e-110 325
MsG0480021961.01.T01 AT1G47128 51.780 309 136 7 40 342 50 351 6.90e-110 328
MsG0480021961.01.T01 AT1G29080 45.481 343 164 7 16 344 13 346 3.89e-106 315
MsG0480021961.01.T01 AT1G09850 50.323 310 150 4 34 342 26 332 1.25e-103 311
MsG0480021961.01.T01 AT4G23520 48.544 309 144 8 41 342 48 348 6.16e-100 299
MsG0480021961.01.T01 AT4G11310 47.910 311 144 7 41 342 50 351 1.28e-97 293
MsG0480021961.01.T01 AT4G11320 46.349 315 143 7 41 342 57 358 1.48e-96 291
MsG0480021961.01.T01 AT4G11320 46.349 315 143 7 41 342 57 358 1.48e-96 291
MsG0480021961.01.T01 AT1G29110 41.311 351 168 9 9 343 5 333 1.10e-87 267
MsG0480021961.01.T01 AT3G43960 43.505 331 179 7 17 342 18 345 4.58e-85 262
MsG0480021961.01.T01 AT4G35350 47.842 278 128 8 10 277 16 286 2.60e-83 254
MsG0480021961.01.T01 AT3G19400 48.963 241 119 4 40 277 44 283 1.58e-76 237
MsG0480021961.01.T01 AT5G60360 43.648 307 157 9 42 343 61 356 4.66e-76 238
MsG0480021961.01.T01 AT3G48350 51.394 251 112 7 33 277 31 277 1.18e-75 235
MsG0480021961.01.T01 AT5G60360 43.322 307 157 10 42 343 61 355 1.58e-73 232
MsG0480021961.01.T01 AT3G45310 42.208 308 162 9 42 344 61 357 1.02e-72 229
MsG0480021961.01.T01 AT5G60360 42.761 297 158 8 42 333 61 350 1.03e-71 227
MsG0480021961.01.T01 AT3G45310 41.883 308 162 10 42 344 61 356 3.35e-70 223
MsG0480021961.01.T01 AT2G21430 38.487 304 163 8 47 335 55 349 2.55e-63 206
MsG0480021961.01.T01 AT4G39090 39.610 308 154 11 47 335 58 352 1.06e-62 204
MsG0480021961.01.T01 AT3G54940 38.924 316 155 10 43 335 54 354 2.74e-60 198
MsG0480021961.01.T01 AT4G16190 38.907 311 158 11 45 335 60 358 5.71e-60 197
MsG0480021961.01.T01 AT3G54940 39.301 229 121 5 43 259 54 276 5.95e-45 155
MsG0480021961.01.T01 AT1G02305 29.778 225 130 7 132 335 116 333 7.26e-24 101
MsG0480021961.01.T01 AT4G01610 27.063 303 178 12 66 339 46 334 4.43e-21 93.2
MsG0480021961.01.T01 AT1G02300 29.384 211 123 6 145 335 146 350 6.69e-20 90.1
MsG0480021961.01.T01 AT4G01610 26.733 303 179 12 66 339 46 334 7.71e-20 89.7
MsG0480021961.01.T01 AT2G22160 40.217 92 52 2 55 145 9 98 8.55e-15 70.1

Find 102 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATGATTTAATTGATTAT+TGG 0.217020 4:-66482301 None:intergenic
TACAAAAGTGGTGTCTTTAC+TGG 0.247785 4:+66483207 MsG0480021961.01.T01:CDS
ACTTCCCAGTACTCAACTTA+TGG 0.259340 4:-66482879 None:intergenic
GCATCAATAGCTACGGAAAT+TGG 0.285597 4:-66483162 None:intergenic
GGTAACATAAGCATTAAGTT+TGG 0.327705 4:-66483432 None:intergenic
CTGCAGTGCCTGCTCATTGT+TGG 0.329361 4:-66483121 None:intergenic
AGTTCCGTCAACACCTTGAT+AGG 0.330791 4:-66483037 None:intergenic
TGGGAAGTTGGTCTCTTTAT+CGG 0.332823 4:+66482894 MsG0480021961.01.T01:CDS
CTTGTTATTGCCAGCATTGT+TGG 0.332876 4:-66482514 None:intergenic
GGGGAGAAGAAGGATATATT+AGG 0.344233 4:+66483334 MsG0480021961.01.T01:CDS
TCACTATGGCAAAGTCTATA+AGG 0.352872 4:+66482428 MsG0480021961.01.T01:CDS
ACCTATTGTAGGTTGTTGTT+GGG 0.354827 4:+66482825 MsG0480021961.01.T01:intron
GAATTCATGGGGAACTGATT+GGG 0.355868 4:+66483314 MsG0480021961.01.T01:CDS
ATCCATGCAGTTACCATTAC+CGG 0.356686 4:+66483081 MsG0480021961.01.T01:CDS
AAATTCATCATTCAAAATCA+TGG 0.364764 4:+66482991 MsG0480021961.01.T01:CDS
ATTGGTTGGTTAAGAATTCA+TGG 0.370703 4:+66483301 MsG0480021961.01.T01:CDS
TTGCTCGAATTACAAAGTTT+AGG 0.373232 4:-66483453 None:intergenic
ATTAAATCATAGCATTTCAT+TGG 0.375929 4:+66482311 MsG0480021961.01.T01:CDS
AGGTTGTGAGGGTGGTCTTA+TGG 0.376813 4:+66482957 MsG0480021961.01.T01:CDS
TACCTATTGTAGGTTGTTGT+TGG 0.387068 4:+66482824 MsG0480021961.01.T01:intron
CATTGACCTTGGTTCTTCAC+TGG 0.396716 4:-66482711 None:intergenic
AGAATTCATGGGGAACTGAT+TGG 0.403396 4:+66483313 MsG0480021961.01.T01:CDS
TAACAAGTTAAATGTGAGAA+AGG 0.408216 4:-66482225 None:intergenic
GGAATCCATAAGTTGAGTAC+TGG 0.422547 4:+66482874 MsG0480021961.01.T01:CDS
AGTAGTTGTCTTTGTGATTG+AGG 0.437424 4:-66482625 None:intergenic
AGTGCCTGCTCATTGTTGGC+AGG 0.443934 4:-66483117 None:intergenic
TTTCTCCAATCCACTGCTGA+TGG 0.445961 4:-66482672 None:intergenic
ACTCAACTTATGGATTCCTT+CGG 0.446108 4:-66482869 None:intergenic
ATTCTTAACCAACCAATACT+TGG 0.453395 4:-66483295 None:intergenic
TCATAGCATTTCATTGGCAT+TGG 0.455936 4:+66482317 MsG0480021961.01.T01:CDS
AGGATATATTAGGATGCAAA+GGG 0.460167 4:+66483344 MsG0480021961.01.T01:CDS
GGAACTGATTGGGGAGAAGA+AGG 0.464169 4:+66483324 MsG0480021961.01.T01:CDS
AAGTTTGGAACTTAAGCAGT+AGG 0.478514 4:-66483417 None:intergenic
TGCATCTAGAAACAAATTCA+AGG 0.484430 4:+66482590 MsG0480021961.01.T01:CDS
AGATGCAATGAACTCCTCAT+TGG 0.488280 4:-66482574 None:intergenic
TTGCTCGTGTCTCTCGTACA+TGG 0.488893 4:-66482397 None:intergenic
TCTATAAGGATTCTCAAGAA+AGG 0.490614 4:+66482442 MsG0480021961.01.T01:CDS
TCTATTAGCTACATTCATCA+TGG 0.491432 4:+66483502 MsG0480021961.01.T01:three_prime_UTR
AAGAAAGGGAGAACCGTCTC+AGG 0.495449 4:+66482457 MsG0480021961.01.T01:CDS
TACCCCTATCAAGGTGTTGA+CGG 0.498791 4:+66483033 MsG0480021961.01.T01:CDS
GACATCTTCATACCCGGTAA+TGG 0.502057 4:-66483094 None:intergenic
GGTTACGGTGTCGGTAATGA+TGG 0.503999 4:+66483270 MsG0480021961.01.T01:CDS
CTATAAGGATTCTCAAGAAA+GGG 0.505602 4:+66482443 MsG0480021961.01.T01:CDS
GCAGTGGATTGGAGAAAGAA+AGG 0.507676 4:+66482678 MsG0480021961.01.T01:CDS
AAGGATATATTAGGATGCAA+AGG 0.511028 4:+66483343 MsG0480021961.01.T01:CDS
GATGCTGCTGAAGGACTTTG+TGG 0.515605 4:+66483372 MsG0480021961.01.T01:CDS
TCTCGTACATGGAGTCATCT+TGG 0.518580 4:-66482386 None:intergenic
TAAGTTGAGTACTGGGAAGT+TGG 0.520530 4:+66482882 MsG0480021961.01.T01:CDS
GTGTTCCATCAGCAGTGGAT+TGG 0.522985 4:+66482667 MsG0480021961.01.T01:CDS
GAGCAATGGATGAGTCACTA+TGG 0.524213 4:+66482414 MsG0480021961.01.T01:CDS
TTGGTTGGTTAAGAATTCAT+GGG 0.526242 4:+66483302 MsG0480021961.01.T01:CDS
GACACTTACCACATTGACCT+TGG 0.526963 4:-66482722 None:intergenic
ATTGAAGCTTCCAACAATGC+TGG 0.532605 4:+66482504 MsG0480021961.01.T01:CDS
CTTGTTGATTGTGACACAAA+AGG 0.533495 4:+66482925 MsG0480021961.01.T01:CDS
AATAACAAGTTATACAAACT+TGG 0.534152 4:+66482528 MsG0480021961.01.T01:CDS
TCTGCTGTTGCGGCAACCGA+AGG 0.538022 4:+66482853 MsG0480021961.01.T01:CDS
GTTCCGTCAACACCTTGATA+GGG 0.541629 4:-66483036 None:intergenic
TAATTGATTATTGGCAGCCA+TGG 0.542700 4:-66482292 None:intergenic
GAATCCATAAGTTGAGTACT+GGG 0.546014 4:+66482875 MsG0480021961.01.T01:CDS
GTGCCTGCTCATTGTTGGCA+GGG 0.546537 4:-66483116 None:intergenic
TCCGTAGCTATTGATGCTAG+TGG 0.550787 4:+66483168 MsG0480021961.01.T01:CDS
GGTGTCACTGCTGTAGGTTA+CGG 0.557743 4:+66483255 MsG0480021961.01.T01:CDS
GAAGCTCAATACCCCTATCA+AGG 0.559311 4:+66483024 MsG0480021961.01.T01:CDS
TGTCCCTGCCAACAATGAGC+AGG 0.564412 4:+66483113 MsG0480021961.01.T01:CDS
CCGTAGCTATTGATGCTAGT+GGG 0.567886 4:+66483169 MsG0480021961.01.T01:CDS
GTAATGATGGAACCAAGTAT+TGG 0.568388 4:+66483283 MsG0480021961.01.T01:CDS
TGATGGAACCAAGTATTGGT+TGG 0.568906 4:+66483287 MsG0480021961.01.T01:CDS
AATTCATGGGGAACTGATTG+GGG 0.571940 4:+66483315 MsG0480021961.01.T01:CDS
AGTTTGGAACTTAAGCAGTA+GGG 0.572870 4:-66483416 None:intergenic
AGGGGAGTCGATGCTGCTGA+AGG 0.575211 4:+66483363 MsG0480021961.01.T01:CDS
TCACCCTTCACTAGCTACCA+TGG 0.579484 4:+66482275 MsG0480021961.01.T01:exon
GCAGCCATGGTAGCTAGTGA+AGG 0.582701 4:-66482279 None:intergenic
TTTCTTTGAATATCCTGAGA+CGG 0.585323 4:-66482470 None:intergenic
AGGACTTTGTGGCATTGCAA+TGG 0.587668 4:+66483383 MsG0480021961.01.T01:CDS
GGTGTAGACCAAGGTTGTGA+GGG 0.591635 4:+66482946 MsG0480021961.01.T01:CDS
TAAGACCACCCTCACAACCT+TGG 0.592405 4:-66482954 None:intergenic
AGGTGTAGACCAAGGTTGTG+AGG 0.594253 4:+66482945 MsG0480021961.01.T01:CDS
GTAGACCAAGGTTGTGAGGG+TGG 0.595756 4:+66482949 MsG0480021961.01.T01:CDS
CCCACTAGCATCAATAGCTA+CGG 0.596676 4:-66483169 None:intergenic
GGTAATGGTAACTGCATGGA+TGG 0.597553 4:-66483079 None:intergenic
ACCCGGTAATGGTAACTGCA+TGG 0.598149 4:-66483083 None:intergenic
TGCAAGTGTTCCATCAGCAG+TGG 0.603452 4:+66482662 MsG0480021961.01.T01:CDS
TTCCGTCAACACCTTGATAG+GGG 0.605371 4:-66483035 None:intergenic
GGCAGGGACATCTTCATACC+CGG 0.606676 4:-66483100 None:intergenic
GCATCTAGAAACAAATTCAA+GGG 0.607899 4:+66482591 MsG0480021961.01.T01:CDS
TGTACGAGAGACACGAGCAA+TGG 0.611237 4:+66482400 MsG0480021961.01.T01:CDS
TGGTTCATGTGGAACTGAGT+TGG 0.612813 4:+66483227 MsG0480021961.01.T01:CDS
GACCATGGTGTCACTGCTGT+AGG 0.612821 4:+66483249 MsG0480021961.01.T01:CDS
TCCATGCAGTTACCATTACC+GGG 0.614442 4:+66483082 MsG0480021961.01.T01:CDS
ACTGCTGTAGGTTACGGTGT+CGG 0.617674 4:+66483261 MsG0480021961.01.T01:CDS
TGGTTGGTTAAGAATTCATG+GGG 0.622660 4:+66483303 MsG0480021961.01.T01:CDS
CAGCCATGGTAGCTAGTGAA+GGG 0.622800 4:-66482278 None:intergenic
CAATGAACTCCTCATTGGTG+AGG 0.636198 4:-66482569 None:intergenic
GGTGTCTTTACTGGTTCATG+TGG 0.637602 4:+66483216 MsG0480021961.01.T01:CDS
AACCTACAGCAGTGACACCA+TGG 0.663029 4:-66483251 None:intergenic
ATTTGCAGACCTCACCAATG+AGG 0.664553 4:+66482560 MsG0480021961.01.T01:CDS
GGATATATTAGGATGCAAAG+GGG 0.671421 4:+66483345 MsG0480021961.01.T01:CDS
GTGAAGAACCAAGGTCAATG+TGG 0.671761 4:+66482714 MsG0480021961.01.T01:CDS
GCAGGCACTGCAGAAAGCTG+TGG 0.675386 4:+66483131 MsG0480021961.01.T01:CDS
TGTGGAACTGAGTTGGACCA+TGG 0.679648 4:+66483234 MsG0480021961.01.T01:CDS
GACACAAAAGGTGTAGACCA+AGG 0.707755 4:+66482937 MsG0480021961.01.T01:CDS
GTAACACCAGTGAAGAACCA+AGG 0.718576 4:+66482705 MsG0480021961.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTAATTTACCTATTGT+AGG + Chr4:66482814-66482833 MsG0480021961.01.T01:intron 15.0%
!! AAATTCATCATTCAAAATCA+TGG + Chr4:66482991-66483010 MsG0480021961.01.T01:CDS 20.0%
!! AATAACAAGTTATACAAACT+TGG + Chr4:66482528-66482547 MsG0480021961.01.T01:CDS 20.0%
!! ATTAAATCATAGCATTTCAT+TGG + Chr4:66482311-66482330 MsG0480021961.01.T01:CDS 20.0%
!! GCTATGATTTAATTGATTAT+TGG - Chr4:66482304-66482323 None:intergenic 20.0%
! TAACAAGTTAAATGTGAGAA+AGG - Chr4:66482228-66482247 None:intergenic 25.0%
! TTAGATCACATAGTATGTAA+TGG - Chr4:66482768-66482787 None:intergenic 25.0%
AAGGATATATTAGGATGCAA+AGG + Chr4:66483343-66483362 MsG0480021961.01.T01:CDS 30.0%
AGGATATATTAGGATGCAAA+GGG + Chr4:66483344-66483363 MsG0480021961.01.T01:CDS 30.0%
ATTCTTAACCAACCAATACT+TGG - Chr4:66483298-66483317 None:intergenic 30.0%
CTATAAGGATTCTCAAGAAA+GGG + Chr4:66482443-66482462 MsG0480021961.01.T01:CDS 30.0%
GCATCTAGAAACAAATTCAA+GGG + Chr4:66482591-66482610 MsG0480021961.01.T01:CDS 30.0%
GGTAACATAAGCATTAAGTT+TGG - Chr4:66483435-66483454 None:intergenic 30.0%
TCTATAAGGATTCTCAAGAA+AGG + Chr4:66482442-66482461 MsG0480021961.01.T01:CDS 30.0%
TCTATTAGCTACATTCATCA+TGG + Chr4:66483502-66483521 MsG0480021961.01.T01:three_prime_UTR 30.0%
TGCATCTAGAAACAAATTCA+AGG + Chr4:66482590-66482609 MsG0480021961.01.T01:CDS 30.0%
TTGCTCGAATTACAAAGTTT+AGG - Chr4:66483456-66483475 None:intergenic 30.0%
TTTCTTTGAATATCCTGAGA+CGG - Chr4:66482473-66482492 None:intergenic 30.0%
! ATTGGTTGGTTAAGAATTCA+TGG + Chr4:66483301-66483320 MsG0480021961.01.T01:CDS 30.0%
! GACTTTCAGTTTTACAAAAG+TGG + Chr4:66483195-66483214 MsG0480021961.01.T01:CDS 30.0%
! TTGGTTGGTTAAGAATTCAT+GGG + Chr4:66483302-66483321 MsG0480021961.01.T01:CDS 30.0%
ACTCAACTTATGGATTCCTT+CGG - Chr4:66482872-66482891 None:intergenic 35.0%
AGTAGTTGTCTTTGTGATTG+AGG - Chr4:66482628-66482647 None:intergenic 35.0%
GGATATATTAGGATGCAAAG+GGG + Chr4:66483345-66483364 MsG0480021961.01.T01:CDS 35.0%
TAATTGATTATTGGCAGCCA+TGG - Chr4:66482295-66482314 None:intergenic 35.0%
TACAAAAGTGGTGTCTTTAC+TGG + Chr4:66483207-66483226 MsG0480021961.01.T01:CDS 35.0%
TACCTATTGTAGGTTGTTGT+TGG + Chr4:66482824-66482843 MsG0480021961.01.T01:intron 35.0%
TCACTATGGCAAAGTCTATA+AGG + Chr4:66482428-66482447 MsG0480021961.01.T01:CDS 35.0%
TCATAGCATTTCATTGGCAT+TGG + Chr4:66482317-66482336 MsG0480021961.01.T01:CDS 35.0%
! AAGTTTGGAACTTAAGCAGT+AGG - Chr4:66483420-66483439 None:intergenic 35.0%
! ACCTATTGTAGGTTGTTGTT+GGG + Chr4:66482825-66482844 MsG0480021961.01.T01:intron 35.0%
! AGTTTGGAACTTAAGCAGTA+GGG - Chr4:66483419-66483438 None:intergenic 35.0%
! CTTGTTGATTGTGACACAAA+AGG + Chr4:66482925-66482944 MsG0480021961.01.T01:CDS 35.0%
! GAATCCATAAGTTGAGTACT+GGG + Chr4:66482875-66482894 MsG0480021961.01.T01:CDS 35.0%
! GTAATGATGGAACCAAGTAT+TGG + Chr4:66483283-66483302 MsG0480021961.01.T01:CDS 35.0%
! TGGTTGGTTAAGAATTCATG+GGG + Chr4:66483303-66483322 MsG0480021961.01.T01:CDS 35.0%
!!! ATTGGCATTGGTTTTATGCT+TGG + Chr4:66482329-66482348 MsG0480021961.01.T01:CDS 35.0%
!!! TTGGCATTGGTTTTATGCTT+GGG + Chr4:66482330-66482349 MsG0480021961.01.T01:CDS 35.0%
AATTCATGGGGAACTGATTG+GGG + Chr4:66483315-66483334 MsG0480021961.01.T01:CDS 40.0%
ACTTCCCAGTACTCAACTTA+TGG - Chr4:66482882-66482901 None:intergenic 40.0%
AGAATTCATGGGGAACTGAT+TGG + Chr4:66483313-66483332 MsG0480021961.01.T01:CDS 40.0%
AGATGCAATGAACTCCTCAT+TGG - Chr4:66482577-66482596 None:intergenic 40.0%
ATCCATGCAGTTACCATTAC+CGG + Chr4:66483081-66483100 MsG0480021961.01.T01:CDS 40.0%
ATTGAAGCTTCCAACAATGC+TGG + Chr4:66482504-66482523 MsG0480021961.01.T01:CDS 40.0%
GAATTCATGGGGAACTGATT+GGG + Chr4:66483314-66483333 MsG0480021961.01.T01:CDS 40.0%
GCATCAATAGCTACGGAAAT+TGG - Chr4:66483165-66483184 None:intergenic 40.0%
GGGGAGAAGAAGGATATATT+AGG + Chr4:66483334-66483353 MsG0480021961.01.T01:CDS 40.0%
! GGAATCCATAAGTTGAGTAC+TGG + Chr4:66482874-66482893 MsG0480021961.01.T01:CDS 40.0%
! GGTGACAAAGAACTGTTTTC+AGG - Chr4:66482260-66482279 None:intergenic 40.0%
! TGGGAAGTTGGTCTCTTTAT+CGG + Chr4:66482894-66482913 MsG0480021961.01.T01:CDS 40.0%
!! CTTGTTATTGCCAGCATTGT+TGG - Chr4:66482517-66482536 None:intergenic 40.0%
!! TAAGTTGAGTACTGGGAAGT+TGG + Chr4:66482882-66482901 MsG0480021961.01.T01:CDS 40.0%
!! TGATGGAACCAAGTATTGGT+TGG + Chr4:66483287-66483306 MsG0480021961.01.T01:CDS 40.0%
AGGACTTTGTGGCATTGCAA+TGG + Chr4:66483383-66483402 MsG0480021961.01.T01:CDS 45.0%
AGTTCCGTCAACACCTTGAT+AGG - Chr4:66483040-66483059 None:intergenic 45.0%
ATTTGCAGACCTCACCAATG+AGG + Chr4:66482560-66482579 MsG0480021961.01.T01:CDS 45.0%
CAATGAACTCCTCATTGGTG+AGG - Chr4:66482572-66482591 None:intergenic 45.0%
CCCACTAGCATCAATAGCTA+CGG - Chr4:66483172-66483191 None:intergenic 45.0%
CCGTAGCTATTGATGCTAGT+GGG + Chr4:66483169-66483188 MsG0480021961.01.T01:CDS 45.0%
GAAGCTCAATACCCCTATCA+AGG + Chr4:66483024-66483043 MsG0480021961.01.T01:CDS 45.0%
GACACTTACCACATTGACCT+TGG - Chr4:66482725-66482744 None:intergenic 45.0%
GACATCTTCATACCCGGTAA+TGG - Chr4:66483097-66483116 None:intergenic 45.0%
GAGCAATGGATGAGTCACTA+TGG + Chr4:66482414-66482433 MsG0480021961.01.T01:CDS 45.0%
GCCCAACAACAACCTACAAT+AGG - Chr4:66482829-66482848 None:intergenic 45.0%
GTAACACCAGTGAAGAACCA+AGG + Chr4:66482705-66482724 MsG0480021961.01.T01:CDS 45.0%
GTGAAGAACCAAGGTCAATG+TGG + Chr4:66482714-66482733 MsG0480021961.01.T01:CDS 45.0%
GTTCCGTCAACACCTTGATA+GGG - Chr4:66483039-66483058 None:intergenic 45.0%
TCCATGCAGTTACCATTACC+GGG + Chr4:66483082-66483101 MsG0480021961.01.T01:CDS 45.0%
TCCGTAGCTATTGATGCTAG+TGG + Chr4:66483168-66483187 MsG0480021961.01.T01:CDS 45.0%
TCTCGTACATGGAGTCATCT+TGG - Chr4:66482389-66482408 None:intergenic 45.0%
TGGTTCATGTGGAACTGAGT+TGG + Chr4:66483227-66483246 MsG0480021961.01.T01:CDS 45.0%
TTCCGTCAACACCTTGATAG+GGG - Chr4:66483038-66483057 None:intergenic 45.0%
TTTCTCCAATCCACTGCTGA+TGG - Chr4:66482675-66482694 None:intergenic 45.0%
! CATTGACCTTGGTTCTTCAC+TGG - Chr4:66482714-66482733 None:intergenic 45.0%
! GACACAAAAGGTGTAGACCA+AGG + Chr4:66482937-66482956 MsG0480021961.01.T01:CDS 45.0%
! GGTAATGGTAACTGCATGGA+TGG - Chr4:66483082-66483101 None:intergenic 45.0%
! TTGGGCATTTTCTGCTGTTG+CGG + Chr4:66482843-66482862 MsG0480021961.01.T01:CDS 45.0%
!! GCAGTGGATTGGAGAAAGAA+AGG + Chr4:66482678-66482697 MsG0480021961.01.T01:CDS 45.0%
!! GGTGTCTTTACTGGTTCATG+TGG + Chr4:66483216-66483235 MsG0480021961.01.T01:CDS 45.0%
!! TACCCCTATCAAGGTGTTGA+CGG + Chr4:66483033-66483052 MsG0480021961.01.T01:CDS 45.0%
AACCTACAGCAGTGACACCA+TGG - Chr4:66483254-66483273 None:intergenic 50.0%
AAGAAAGGGAGAACCGTCTC+AGG + Chr4:66482457-66482476 MsG0480021961.01.T01:CDS 50.0%
ACTGCTGTAGGTTACGGTGT+CGG + Chr4:66483261-66483280 MsG0480021961.01.T01:CDS 50.0%
AGGTGTAGACCAAGGTTGTG+AGG + Chr4:66482945-66482964 MsG0480021961.01.T01:CDS 50.0%
CAGCCATGGTAGCTAGTGAA+GGG - Chr4:66482281-66482300 None:intergenic 50.0%
GATGCTGCTGAAGGACTTTG+TGG + Chr4:66483372-66483391 MsG0480021961.01.T01:CDS 50.0%
GGAACTGATTGGGGAGAAGA+AGG + Chr4:66483324-66483343 MsG0480021961.01.T01:CDS 50.0%
GGTGTAGACCAAGGTTGTGA+GGG + Chr4:66482946-66482965 MsG0480021961.01.T01:CDS 50.0%
GTGTTCCATCAGCAGTGGAT+TGG + Chr4:66482667-66482686 MsG0480021961.01.T01:CDS 50.0%
TAAGACCACCCTCACAACCT+TGG - Chr4:66482957-66482976 None:intergenic 50.0%
TCACCCTTCACTAGCTACCA+TGG + Chr4:66482275-66482294 MsG0480021961.01.T01:exon 50.0%
TGCAAGTGTTCCATCAGCAG+TGG + Chr4:66482662-66482681 MsG0480021961.01.T01:CDS 50.0%
TGTACGAGAGACACGAGCAA+TGG + Chr4:66482400-66482419 MsG0480021961.01.T01:CDS 50.0%
TTGCTCGTGTCTCTCGTACA+TGG - Chr4:66482400-66482419 None:intergenic 50.0%
! ACCCGGTAATGGTAACTGCA+TGG - Chr4:66483086-66483105 None:intergenic 50.0%
! AGGTTGTGAGGGTGGTCTTA+TGG + Chr4:66482957-66482976 MsG0480021961.01.T01:CDS 50.0%
! TGTGGAACTGAGTTGGACCA+TGG + Chr4:66483234-66483253 MsG0480021961.01.T01:CDS 50.0%
!! GGTGTCACTGCTGTAGGTTA+CGG + Chr4:66483255-66483274 MsG0480021961.01.T01:CDS 50.0%
!! GGTTACGGTGTCGGTAATGA+TGG + Chr4:66483270-66483289 MsG0480021961.01.T01:CDS 50.0%
AGTGCCTGCTCATTGTTGGC+AGG - Chr4:66483120-66483139 None:intergenic 55.0%
CTGCAGTGCCTGCTCATTGT+TGG - Chr4:66483124-66483143 None:intergenic 55.0%
GCAGCCATGGTAGCTAGTGA+AGG - Chr4:66482282-66482301 None:intergenic 55.0%
GGCAGGGACATCTTCATACC+CGG - Chr4:66483103-66483122 None:intergenic 55.0%
GTAGACCAAGGTTGTGAGGG+TGG + Chr4:66482949-66482968 MsG0480021961.01.T01:CDS 55.0%
GTGCCTGCTCATTGTTGGCA+GGG - Chr4:66483119-66483138 None:intergenic 55.0%
TGTCCCTGCCAACAATGAGC+AGG + Chr4:66483113-66483132 MsG0480021961.01.T01:CDS 55.0%
!! GACCATGGTGTCACTGCTGT+AGG + Chr4:66483249-66483268 MsG0480021961.01.T01:CDS 55.0%
AGGGGAGTCGATGCTGCTGA+AGG + Chr4:66483363-66483382 MsG0480021961.01.T01:CDS 60.0%
TCTGCTGTTGCGGCAACCGA+AGG + Chr4:66482853-66482872 MsG0480021961.01.T01:CDS 60.0%
! GCAGGCACTGCAGAAAGCTG+TGG + Chr4:66483131-66483150 MsG0480021961.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 66482217 66483570 66482217 ID=MsG0480021961.01;Name=MsG0480021961.01
Chr4 mRNA 66482217 66483570 66482217 ID=MsG0480021961.01.T01;Parent=MsG0480021961.01;Name=MsG0480021961.01.T01;_AED=0.08;_eAED=0.08;_QI=77|1|1|1|1|1|2|142|344
Chr4 exon 66482217 66482735 66482217 ID=MsG0480021961.01.T01:exon:18225;Parent=MsG0480021961.01.T01
Chr4 exon 66482836 66483570 66482836 ID=MsG0480021961.01.T01:exon:18226;Parent=MsG0480021961.01.T01
Chr4 five_prime_UTR 66482217 66482293 66482217 ID=MsG0480021961.01.T01:five_prime_utr;Parent=MsG0480021961.01.T01
Chr4 CDS 66482294 66482735 66482294 ID=MsG0480021961.01.T01:cds;Parent=MsG0480021961.01.T01
Chr4 CDS 66482836 66483428 66482836 ID=MsG0480021961.01.T01:cds;Parent=MsG0480021961.01.T01
Chr4 three_prime_UTR 66483429 66483570 66483429 ID=MsG0480021961.01.T01:three_prime_utr;Parent=MsG0480021961.01.T01
Gene Sequence

>MsG0480021961.01.T01

ATGGCTGCCAATAATCAATTAAATCATAGCATTTCATTGGCATTGGTTTTATGCTTGGGATTGTTTGCTATTCAAGTCACTTCTCGTACTCTCCAAGATGACTCCATGTACGAGAGACACGAGCAATGGATGAGTCACTATGGCAAAGTCTATAAGGATTCTCAAGAAAGGGAGAACCGTCTCAGGATATTCAAAGAAAATGTGAATTACATTGAAGCTTCCAACAATGCTGGCAATAACAAGTTATACAAACTTGGCATTAATCAATTTGCAGACCTCACCAATGAGGAGTTCATTGCATCTAGAAACAAATTCAAGGGTCATATGTGCTCCTCAATCACAAAGACAACTACTTTTATGTATGAAAATGCAAGTGTTCCATCAGCAGTGGATTGGAGAAAGAAAGGAGCAGTAACACCAGTGAAGAACCAAGGTCAATGTGGTTGTTGTTGGGCATTTTCTGCTGTTGCGGCAACCGAAGGAATCCATAAGTTGAGTACTGGGAAGTTGGTCTCTTTATCGGAACAAGAACTTGTTGATTGTGACACAAAAGGTGTAGACCAAGGTTGTGAGGGTGGTCTTATGGATGATGCTTTCAAATTCATCATTCAAAATCATGGACTCAACACTGAAGCTCAATACCCCTATCAAGGTGTTGACGGAACTTGCAGTGCAAACAAAGCATCCATCCATGCAGTTACCATTACCGGGTATGAAGATGTCCCTGCCAACAATGAGCAGGCACTGCAGAAAGCTGTGGCAAATCAACCAATTTCCGTAGCTATTGATGCTAGTGGGTCTGACTTTCAGTTTTACAAAAGTGGTGTCTTTACTGGTTCATGTGGAACTGAGTTGGACCATGGTGTCACTGCTGTAGGTTACGGTGTCGGTAATGATGGAACCAAGTATTGGTTGGTTAAGAATTCATGGGGAACTGATTGGGGAGAAGAAGGATATATTAGGATGCAAAGGGGAGTCGATGCTGCTGAAGGACTTTGTGGCATTGCAATGGAAGCATCTTACCCTACTGCTTAA
Protein sequence

>MsG0480021961.01.T01

MAANNQLNHSISLALVLCLGLFAIQVTSRTLQDDSMYERHEQWMSHYGKVYKDSQERENRLRIFKENVNYIEASNNAGNNKLYKLGINQFADLTNEEFIASRNKFKGHMCSSITKTTTFMYENASVPSAVDWRKKGAVTPVKNQGQCGCCWAFSAVAATEGIHKLSTGKLVSLSEQELVDCDTKGVDQGCEGGLMDDAFKFIIQNHGLNTEAQYPYQGVDGTCSANKASIHAVTITGYEDVPANNEQALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGVGNDGTKYWLVKNSWGTDWGEEGYIRMQRGVDAAEGLCGIAMEASYPTA*