AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021964.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021964.01.T01 MTR_4g079770 87.608 347 39 2 1 345 1 345 0.0 642
MsG0480021964.01.T01 MTR_4g079800 89.625 347 32 2 1 345 1 345 0.0 621
MsG0480021964.01.T01 MTR_4g079470 88.473 347 36 2 1 345 1 345 0.0 611
MsG0480021964.01.T01 MTR_5g022560 83.046 348 52 4 1 345 1 344 0.0 605
MsG0480021964.01.T01 MTR_4g107930 82.471 348 55 4 1 345 1 345 0.0 597
MsG0480021964.01.T01 MTR_4g047610 84.770 348 45 4 1 345 1 343 0.0 591
MsG0480021964.01.T01 MTR_2g075830 73.775 347 83 5 1 345 1 341 0.0 519
MsG0480021964.01.T01 MTR_4g080700 64.308 325 102 7 26 345 25 340 8.24e-152 431
MsG0480021964.01.T01 MTR_4g080730 64.308 325 102 7 26 345 25 340 1.12e-150 427
MsG0480021964.01.T01 MTR_4g104370 56.012 341 135 9 11 343 9 342 7.89e-131 378
MsG0480021964.01.T01 MTR_4g080360 52.149 349 156 6 1 345 1 342 5.38e-129 373
MsG0480021964.01.T01 MTR_3g075390 52.353 340 152 7 13 344 7 344 9.51e-129 373
MsG0480021964.01.T01 MTR_2g083930 51.852 351 152 5 1 343 1 342 6.85e-126 365
MsG0480021964.01.T01 MTR_3g116080 50.578 346 156 7 7 344 9 347 1.47e-120 352
MsG0480021964.01.T01 MTR_4g079440 53.548 310 139 3 35 343 38 343 2.88e-120 350
MsG0480021964.01.T01 MTR_7g078600 54.232 319 131 7 31 343 27 336 5.32e-118 345
MsG0480021964.01.T01 MTR_2g090890 49.112 338 164 4 10 344 4 336 4.64e-117 342
MsG0480021964.01.T01 MTR_4g094918 49.708 342 162 7 10 344 3 341 1.06e-116 342
MsG0480021964.01.T01 MTR_1g107550 54.430 316 134 6 35 343 42 354 2.94e-116 345
MsG0480021964.01.T01 MTR_7g079230 51.351 333 152 7 14 343 9 334 2.12e-113 333
MsG0480021964.01.T01 MTR_1g018840 47.790 362 163 8 4 343 3 360 1.43e-110 330
MsG0480021964.01.T01 MTR_1g018840 47.790 362 163 8 4 343 3 360 1.87e-110 330
MsG0480021964.01.T01 MTR_3g102220 50.625 320 141 7 36 343 46 360 1.07e-108 326
MsG0480021964.01.T01 MTR_3g112430 49.677 310 142 5 44 344 37 341 2.82e-100 303
MsG0480021964.01.T01 MTR_2g015850 53.952 291 126 6 57 343 76 362 1.38e-99 298
MsG0480021964.01.T01 MTR_2g016180 46.686 347 162 10 12 344 18 355 2.57e-91 281
MsG0480021964.01.T01 MTR_2g016270 46.356 343 154 10 12 344 18 340 3.12e-89 276
MsG0480021964.01.T01 MTR_2g067900 44.304 316 154 12 42 342 15 323 4.27e-74 232
MsG0480021964.01.T01 MTR_4g125300 41.722 302 162 7 49 345 57 349 2.74e-73 231
MsG0480021964.01.T01 MTR_2g067560 44.201 319 146 13 42 338 15 323 4.89e-71 238
MsG0480021964.01.T01 MTR_2g015920 37.746 355 191 14 11 344 13 358 3.82e-70 223
MsG0480021964.01.T01 MTR_2g064475 39.080 348 156 15 42 344 15 351 1.22e-69 221
MsG0480021964.01.T01 MTR_4g071060 37.255 357 187 10 14 341 5 353 2.93e-68 218
MsG0480021964.01.T01 MTR_4g065077 38.509 322 176 9 36 344 44 356 1.97e-65 211
MsG0480021964.01.T01 MTR_2g066980 37.168 339 150 13 28 344 21 318 1.45e-64 207
MsG0480021964.01.T01 MTR_7g078630 44.170 283 118 7 33 304 697 950 1.90e-64 220
MsG0480021964.01.T01 MTR_2g015930 36.712 365 198 13 3 344 4 358 1.28e-63 206
MsG0480021964.01.T01 MTR_1g023210 37.048 332 169 12 35 341 41 357 2.20e-63 206
MsG0480021964.01.T01 MTR_1g023810 44.589 231 119 6 115 342 37 261 2.42e-62 200
MsG0480021964.01.T01 MTR_8g086470 37.421 318 169 9 36 344 60 356 8.65e-61 199
MsG0480021964.01.T01 MTR_1g094135 36.257 342 181 13 24 341 35 363 4.61e-57 190
MsG0480021964.01.T01 MTR_4g072790 32.464 345 184 12 12 341 7 317 2.40e-45 158
MsG0480021964.01.T01 MTR_4g072340 30.168 358 196 10 1 341 1 321 6.36e-45 157
MsG0480021964.01.T01 MTR_4g073660 31.034 348 189 10 12 341 6 320 1.27e-44 156
MsG0480021964.01.T01 MTR_7g078580 65.306 98 33 1 246 343 4 100 1.42e-41 142
MsG0480021964.01.T01 MTR_7g079130 71.111 90 26 0 254 343 7 96 7.98e-41 139
MsG0480021964.01.T01 MTR_2g015840 46.875 160 77 5 7 162 1 156 8.60e-38 136
MsG0480021964.01.T01 MTR_2g015840 40.201 199 99 6 164 344 75 271 7.81e-37 134
MsG0480021964.01.T01 MTR_1g025450 37.433 187 92 6 115 297 43 208 1.55e-32 127
MsG0480021964.01.T01 MTR_2g016380 48.718 117 55 3 231 344 21 135 5.39e-28 110
MsG0480021964.01.T01 MTR_7g078310 42.143 140 71 3 84 220 115 247 2.39e-26 105
MsG0480021964.01.T01 MTR_3g029560 30.556 252 138 9 100 344 16 237 1.97e-23 97.4
MsG0480021964.01.T01 MTR_7g111060 25.161 310 193 10 59 336 28 330 2.48e-22 96.7
MsG0480021964.01.T01 MTR_7g079260 61.111 72 12 1 270 341 7 62 3.16e-22 90.5
MsG0480021964.01.T01 MTR_7g111050 28.511 235 138 8 133 344 110 337 1.09e-21 94.7
MsG0480021964.01.T01 MTR_8g087510 39.695 131 72 5 168 296 23 148 3.03e-19 84.3
MsG0480021964.01.T01 MTR_2g047960 48.235 85 43 1 136 220 7 90 1.84e-18 79.7
MsG0480021964.01.T01 MTR_7g111050 26.154 195 120 6 133 306 105 296 1.72e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021964.01.T01 AT5G45890 55.263 342 141 6 13 344 6 345 4.25e-134 386
MsG0480021964.01.T01 AT5G50260 53.687 339 145 7 15 343 5 341 3.49e-130 376
MsG0480021964.01.T01 AT3G48340 52.874 348 141 9 13 343 1 342 1.19e-124 362
MsG0480021964.01.T01 AT2G34080 50.292 342 159 6 11 345 8 345 3.26e-121 353
MsG0480021964.01.T01 AT4G35350 51.009 347 154 8 7 344 13 352 2.38e-119 348
MsG0480021964.01.T01 AT3G19390 52.994 334 149 6 14 344 17 345 6.49e-119 351
MsG0480021964.01.T01 AT3G48350 50.578 346 156 8 11 344 1 343 9.42e-118 345
MsG0480021964.01.T01 AT3G49340 49.565 345 151 8 17 345 4 341 2.22e-116 340
MsG0480021964.01.T01 AT1G06260 49.704 338 161 5 10 343 9 341 2.93e-114 335
MsG0480021964.01.T01 AT1G20850 50.000 326 151 6 26 344 33 353 6.05e-114 335
MsG0480021964.01.T01 AT2G27420 48.148 351 163 6 11 345 1 348 7.91e-114 334
MsG0480021964.01.T01 AT3G19400 51.307 306 144 5 43 344 44 348 1.67e-111 329
MsG0480021964.01.T01 AT5G43060 53.896 308 130 5 43 343 50 352 1.73e-111 332
MsG0480021964.01.T01 AT1G47128 52.614 306 136 5 43 343 50 351 8.35e-111 331
MsG0480021964.01.T01 AT1G29090 46.839 348 170 7 7 345 14 355 5.99e-110 325
MsG0480021964.01.T01 AT4G36880 50.769 325 139 7 36 344 42 361 3.02e-109 324
MsG0480021964.01.T01 AT1G29080 45.402 348 167 7 13 345 7 346 2.19e-106 315
MsG0480021964.01.T01 AT4G23520 43.714 350 176 8 9 343 5 348 6.86e-102 304
MsG0480021964.01.T01 AT1G09850 49.180 305 151 4 41 343 30 332 3.55e-99 300
MsG0480021964.01.T01 AT4G11310 45.955 309 151 6 44 343 50 351 4.73e-99 297
MsG0480021964.01.T01 AT4G11320 44.728 313 149 6 44 343 57 358 1.57e-97 293
MsG0480021964.01.T01 AT4G11320 44.728 313 149 6 44 343 57 358 1.57e-97 293
MsG0480021964.01.T01 AT1G29110 41.834 349 164 9 11 344 9 333 3.15e-89 271
MsG0480021964.01.T01 AT3G43960 43.769 329 175 8 22 343 20 345 2.34e-87 268
MsG0480021964.01.T01 AT4G35350 47.331 281 132 8 7 278 13 286 2.68e-83 254
MsG0480021964.01.T01 AT5G60360 44.040 302 155 8 49 345 65 357 5.35e-79 246
MsG0480021964.01.T01 AT3G48350 46.786 280 134 8 11 278 1 277 1.06e-78 243
MsG0480021964.01.T01 AT5G60360 43.377 302 156 9 49 345 65 356 2.53e-76 239
MsG0480021964.01.T01 AT3G45310 40.237 338 174 12 24 345 32 357 4.09e-76 238
MsG0480021964.01.T01 AT3G19400 48.750 240 119 4 43 278 44 283 4.31e-76 236
MsG0480021964.01.T01 AT5G60360 42.568 296 160 7 49 339 65 355 2.22e-74 234
MsG0480021964.01.T01 AT3G45310 39.941 338 174 13 24 345 32 356 1.80e-73 231
MsG0480021964.01.T01 AT2G21430 35.474 327 185 6 29 341 40 354 1.63e-63 206
MsG0480021964.01.T01 AT4G39090 37.419 310 166 8 50 341 58 357 1.03e-61 201
MsG0480021964.01.T01 AT4G16190 37.299 311 167 8 50 341 62 363 1.87e-59 196
MsG0480021964.01.T01 AT3G54940 35.695 367 191 11 7 341 6 359 3.06e-59 195
MsG0480021964.01.T01 AT3G54940 37.662 231 128 4 42 260 50 276 2.19e-42 149
MsG0480021964.01.T01 AT1G02305 27.213 305 173 13 75 344 53 343 4.87e-24 101
MsG0480021964.01.T01 AT4G01610 27.241 290 174 11 75 336 50 330 1.98e-22 97.1
MsG0480021964.01.T01 AT4G01610 26.129 310 191 12 55 336 31 330 3.29e-21 93.6
MsG0480021964.01.T01 AT1G02300 28.251 223 128 8 146 344 146 360 5.88e-19 87.4
MsG0480021964.01.T01 AT2G22160 41.584 101 55 2 47 146 1 98 2.22e-18 80.1

Find 93 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGCATAATTTACATAGTT+TGG 0.205834 4:-66525753 None:intergenic
TGTATTTATTAATACAATTA+AGG 0.218245 4:+66525801 MsG0480021964.01.T01:three_prime_UTR
TTGATTAATTTATCAGTTAA+AGG 0.219631 4:-66524517 None:intergenic
AAATTCATCATTCAAAATAA+TGG 0.238434 4:+66525290 MsG0480021964.01.T01:CDS
ACCCCTGGTGCTACCATTAC+TGG 0.265388 4:+66525380 MsG0480021964.01.T01:CDS
TGTTCCATCAACACCTTGAT+AGG 0.273901 4:-66525336 None:intergenic
GCATCAATGGCTACAGAAAT+TGG 0.275164 4:-66525461 None:intergenic
TAGCATAATTTACATAGTTT+GGG 0.277970 4:-66525752 None:intergenic
GCAATGATGGAACCAAATAT+TGG 0.324413 4:+66525582 MsG0480021964.01.T01:CDS
TCACTATGGCAAAGTATATA+AGG 0.341535 4:+66524732 MsG0480021964.01.T01:CDS
GGGGAGAAGAAGGATATATT+AGG 0.344233 4:+66525633 MsG0480021964.01.T01:CDS
AAACTCATGGGGAACTGATT+GGG 0.355728 4:+66525613 MsG0480021964.01.T01:CDS
GTATGGCTTGTTAGCATTGT+TGG 0.355877 4:-66524818 None:intergenic
AACACCTTGATAGGGGTATT+CGG 0.374376 4:-66525327 None:intergenic
AGGTTGTGAGGGTGGCCTTA+TGG 0.383116 4:+66525256 MsG0480021964.01.T01:CDS
GGTGTTACTGCTGTGGGTTA+TGG 0.385581 4:+66525554 MsG0480021964.01.T01:CDS
TGATTAATGCCAAGCTTGTA+TGG 0.387555 4:-66524835 None:intergenic
TGAGAACCAACAAGTGCTTC+AGG 0.398302 4:-66524540 None:intergenic
TGCTCGTGTTTCTCATACAT+GGG 0.399669 4:-66524700 None:intergenic
TTGCTCGTGTTTCTCATACA+TGG 0.405192 4:-66524701 None:intergenic
AATCAACCTGAAGCACTTGT+TGG 0.407360 4:+66524534 MsG0480021964.01.T01:five_prime_UTR
AAAACTCATGGGGAACTGAT+TGG 0.415777 4:+66525612 MsG0480021964.01.T01:CDS
TACCCCTATCAAGGTGTTGA+TGG 0.422220 4:+66525332 MsG0480021964.01.T01:CDS
TGCCTTTATAGGATGTTGTT+GGG 0.424657 4:+66525124 MsG0480021964.01.T01:intron
ATGCCTTTATAGGATGTTGT+TGG 0.435997 4:+66525123 MsG0480021964.01.T01:intron
TTTCTTCCTCCAATCTATTG+TGG 0.440357 4:-66524974 None:intergenic
TGGGGTGGTCATCTAAATCT+TGG 0.450516 4:-66524681 None:intergenic
TACAAAAGTGGCGTCTTCAC+TGG 0.456462 4:+66525506 MsG0480021964.01.T01:CDS
ATCCGAGCCACTCGCATCAA+TGG 0.460166 4:-66525474 None:intergenic
TAATTGATTATTTGTAGTCA+TGG 0.460863 4:-66524587 None:intergenic
GAGCAATGGATGGGTCACTA+TGG 0.461657 4:+66524718 MsG0480021964.01.T01:CDS
GGAACTGATTGGGGAGAAGA+AGG 0.464169 4:+66525623 MsG0480021964.01.T01:CDS
GGGGTTCATAAGTTAAGTAC+TGG 0.468533 4:+66525173 MsG0480021964.01.T01:CDS
ACTTTGTGGCATTGCAATGA+TGG 0.469959 4:+66525685 MsG0480021964.01.T01:CDS
TTCCTCCAATCTATTGTGGA+TGG 0.471216 4:-66524970 None:intergenic
TTCTTATCCCACTGCTTGAA+AGG 0.478972 4:+66525709 MsG0480021964.01.T01:exon
ACATTGACCTTGATTCTTCA+CGG 0.480868 4:-66525010 None:intergenic
CAGCTATTGCCGCAACTGAA+GGG 0.483577 4:+66525153 MsG0480021964.01.T01:CDS
CATTGACCTTGATTCTTCAC+GGG 0.485402 4:-66525009 None:intergenic
TCAGCTATTGCCGCAACTGA+AGG 0.487691 4:+66525152 MsG0480021964.01.T01:CDS
AGGATATATTAGGATGCAAA+GGG 0.491081 4:+66525643 MsG0480021964.01.T01:CDS
CCCCTGGTGCTACCATTACT+GGG 0.500770 4:+66525381 MsG0480021964.01.T01:CDS
ACAATAGATTGGAGGAAGAA+AGG 0.501642 4:+66524976 MsG0480021964.01.T01:CDS
TGCTACCATTACTGGGTATG+AGG 0.503327 4:+66525388 MsG0480021964.01.T01:CDS
ATTTGAAAGCATCATCCATA+AGG 0.506586 4:-66525271 None:intergenic
GATGCTGCTGAAGGACTTTG+TGG 0.509467 4:+66525671 MsG0480021964.01.T01:CDS
AAGGATATATTAGGATGCAA+AGG 0.511438 4:+66525642 MsG0480021964.01.T01:CDS
AGTGCATTCTCATTGTTTGT+TGG 0.516355 4:-66525416 None:intergenic
AAGTTTGCCTTTCAAGCAGT+GGG 0.516862 4:-66525716 None:intergenic
GTGTTCCATCCACAATAGAT+TGG 0.520539 4:+66524965 MsG0480021964.01.T01:CDS
GGTTATGGCATTAGCAATGA+TGG 0.526117 4:+66525569 MsG0480021964.01.T01:CDS
CTTGTTGATTGTGACACAAA+AGG 0.533495 4:+66525224 MsG0480021964.01.T01:CDS
GTTCCATCAACACCTTGATA+GGG 0.537305 4:-66525335 None:intergenic
GGATCATGGTGTTACTGCTG+TGG 0.544384 4:+66525547 MsG0480021964.01.T01:CDS
GTAACACCCGTGAAGAATCA+AGG 0.545245 4:+66525003 MsG0480021964.01.T01:CDS
CAAGTTTGCCTTTCAAGCAG+TGG 0.545377 4:-66525717 None:intergenic
GCTAACAAGCCATACAAGCT+TGG 0.545790 4:+66524826 MsG0480021964.01.T01:CDS
TACCCAGTAATGGTAGCACC+AGG 0.545824 4:-66525383 None:intergenic
TGTATGAGAAACACGAGCAA+TGG 0.546417 4:+66524704 MsG0480021964.01.T01:CDS
TGATGGAACCAAATATTGGT+TGG 0.547511 4:+66525586 MsG0480021964.01.T01:CDS
TGAGAAACACGAGCAATGGA+TGG 0.547841 4:+66524708 MsG0480021964.01.T01:CDS
AACTCATGGGGAACTGATTG+GGG 0.548211 4:+66525614 MsG0480021964.01.T01:CDS
GATCATGGTGTTACTGCTGT+GGG 0.556273 4:+66525548 MsG0480021964.01.T01:CDS
AGCCATTGATGCGAGTGGCT+CGG 0.560045 4:+66525472 MsG0480021964.01.T01:CDS
CTTATGAACCCCTTCAGTTG+CGG 0.560982 4:-66525162 None:intergenic
AGCATAATTTACATAGTTTG+GGG 0.560986 4:-66525751 None:intergenic
TGTGGAACTGAGTTGGATCA+TGG 0.564256 4:+66525533 MsG0480021964.01.T01:CDS
CCCAGTAATGGTAGCACCAG+GGG 0.565986 4:-66525381 None:intergenic
GTAGTCATGGTAATTAGTGA+AGG 0.566689 4:-66524574 None:intergenic
GGCGTCTTCACTGGTTCATG+TGG 0.573359 4:+66525515 MsG0480021964.01.T01:CDS
GCAAATGAAACTTCAACCCC+TGG 0.576141 4:+66525365 MsG0480021964.01.T01:CDS
GACACAAAAGGTGAAGATCA+AGG 0.577927 4:+66525236 MsG0480021964.01.T01:CDS
GGTGAAGATCAAGGTTGTGA+GGG 0.583430 4:+66525245 MsG0480021964.01.T01:CDS
GGATGATGTGAATTCCTCGT+TGG 0.583508 4:-66524872 None:intergenic
GAAGATCAAGGTTGTGAGGG+TGG 0.583609 4:+66525248 MsG0480021964.01.T01:CDS
TTCCATCCACAATAGATTGG+AGG 0.585230 4:+66524968 MsG0480021964.01.T01:CDS
AGCTATTGCCGCAACTGAAG+GGG 0.591317 4:+66525154 MsG0480021964.01.T01:CDS
GAAACCGAATACCCCTATCA+AGG 0.596100 4:+66525323 MsG0480021964.01.T01:CDS
TTCCATCAACACCTTGATAG+GGG 0.602425 4:-66525334 None:intergenic
CGTGTTTCTCATACATGGGG+TGG 0.606141 4:-66524696 None:intergenic
GAGAAACACGAGCAATGGAT+GGG 0.609591 4:+66524709 MsG0480021964.01.T01:CDS
AACATCCTCATACCCAGTAA+TGG 0.610351 4:-66525393 None:intergenic
TGGTTCATGTGGAACTGAGT+TGG 0.610825 4:+66525526 MsG0480021964.01.T01:CDS
ACATGTGTTCTTCTATCACT+AGG 0.613736 4:+66524914 MsG0480021964.01.T01:CDS
AGGTGAAGATCAAGGTTGTG+AGG 0.623389 4:+66525244 MsG0480021964.01.T01:CDS
TCTGTAGCCATTGATGCGAG+TGG 0.627989 4:+66525467 MsG0480021964.01.T01:CDS
AGGGGTGTAGATGCTGCTGA+AGG 0.630278 4:+66525662 MsG0480021964.01.T01:CDS
ATGTGAATTCCTCGTTGGTG+AGG 0.632347 4:-66524867 None:intergenic
GCTCGTGTTTCTCATACATG+GGG 0.638157 4:-66524699 None:intergenic
ACCCAGTAATGGTAGCACCA+GGG 0.669685 4:-66525382 None:intergenic
GGATATATTAGGATGCAAAG+GGG 0.706332 4:+66525644 MsG0480021964.01.T01:CDS
GTGAAGAATCAAGGTCAATG+TGG 0.717596 4:+66525012 MsG0480021964.01.T01:CDS
ATTTGCAGACCTCACCAACG+AGG 0.759666 4:+66524858 MsG0480021964.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTATTTATTAATACAATTA+AGG + Chr4:66525801-66525820 MsG0480021964.01.T01:three_prime_UTR 10.0%
!! AAATTCATCATTCAAAATAA+TGG + Chr4:66525290-66525309 MsG0480021964.01.T01:CDS 15.0%
!! TTGATTAATTTATCAGTTAA+AGG - Chr4:66524520-66524539 None:intergenic 15.0%
!!! AAAGGCATATTTAAAATAAT+TGG - Chr4:66525111-66525130 None:intergenic 15.0%
!!! TATTTTAAATATGCCTTTAT+AGG + Chr4:66525113-66525132 MsG0480021964.01.T01:intron 15.0%
!!! TTGAATTCCTTTAATTTATA+CGG + Chr4:66525061-66525080 MsG0480021964.01.T01:intron 15.0%
!! AACTTAACCGTATAAATTAA+AGG - Chr4:66525071-66525090 None:intergenic 20.0%
!! TAATTGATTATTTGTAGTCA+TGG - Chr4:66524590-66524609 None:intergenic 20.0%
!! TAGCATAATTTACATAGTTT+GGG - Chr4:66525755-66525774 None:intergenic 20.0%
!! TTAGCATAATTTACATAGTT+TGG - Chr4:66525756-66525775 None:intergenic 20.0%
! AGCATAATTTACATAGTTTG+GGG - Chr4:66525754-66525773 None:intergenic 25.0%
! ATCATCCAAAAATAGATTCA+AGG + Chr4:66524888-66524907 MsG0480021964.01.T01:CDS 25.0%
! TCATCCAAAAATAGATTCAA+GGG + Chr4:66524889-66524908 MsG0480021964.01.T01:CDS 25.0%
!! GATTTCCAATTTTACAAAAG+TGG + Chr4:66525494-66525513 MsG0480021964.01.T01:CDS 25.0%
!! GTTTTTAACCAACCAATATT+TGG - Chr4:66525597-66525616 None:intergenic 25.0%
AAGGATATATTAGGATGCAA+AGG + Chr4:66525642-66525661 MsG0480021964.01.T01:CDS 30.0%
AGGATATATTAGGATGCAAA+GGG + Chr4:66525643-66525662 MsG0480021964.01.T01:CDS 30.0%
CATCCAAAAATAGATTCAAG+GGG + Chr4:66524890-66524909 MsG0480021964.01.T01:CDS 30.0%
TCACTATGGCAAAGTATATA+AGG + Chr4:66524732-66524751 MsG0480021964.01.T01:CDS 30.0%
! ATTGGTTGGTTAAAAACTCA+TGG + Chr4:66525600-66525619 MsG0480021964.01.T01:CDS 30.0%
! TTGGTTGGTTAAAAACTCAT+GGG + Chr4:66525601-66525620 MsG0480021964.01.T01:CDS 30.0%
!! ATTGACATTGCTTTTATGCT+TGG + Chr4:66524624-66524643 MsG0480021964.01.T01:CDS 30.0%
!! ATTTGAAAGCATCATCCATA+AGG - Chr4:66525274-66525293 None:intergenic 30.0%
!! TTGACATTGCTTTTATGCTT+GGG + Chr4:66524625-66524644 MsG0480021964.01.T01:CDS 30.0%
ACAATAGATTGGAGGAAGAA+AGG + Chr4:66524976-66524995 MsG0480021964.01.T01:CDS 35.0%
ACATGTGTTCTTCTATCACT+AGG + Chr4:66524914-66524933 MsG0480021964.01.T01:CDS 35.0%
ACATTGACCTTGATTCTTCA+CGG - Chr4:66525013-66525032 None:intergenic 35.0%
AGTGCATTCTCATTGTTTGT+TGG - Chr4:66525419-66525438 None:intergenic 35.0%
ATGCCTTTATAGGATGTTGT+TGG + Chr4:66525123-66525142 MsG0480021964.01.T01:intron 35.0%
GGATATATTAGGATGCAAAG+GGG + Chr4:66525644-66525663 MsG0480021964.01.T01:CDS 35.0%
GTAGTCATGGTAATTAGTGA+AGG - Chr4:66524577-66524596 None:intergenic 35.0%
TGATGGAACCAAATATTGGT+TGG + Chr4:66525586-66525605 MsG0480021964.01.T01:CDS 35.0%
TGATTAATGCCAAGCTTGTA+TGG - Chr4:66524838-66524857 None:intergenic 35.0%
TGCCTTTATAGGATGTTGTT+GGG + Chr4:66525124-66525143 MsG0480021964.01.T01:intron 35.0%
TTTCTTCCTCCAATCTATTG+TGG - Chr4:66524977-66524996 None:intergenic 35.0%
! CTTGTTGATTGTGACACAAA+AGG + Chr4:66525224-66525243 MsG0480021964.01.T01:CDS 35.0%
! GCAATGATGGAACCAAATAT+TGG + Chr4:66525582-66525601 MsG0480021964.01.T01:CDS 35.0%
! TGGTTGGTTAAAAACTCATG+GGG + Chr4:66525602-66525621 MsG0480021964.01.T01:CDS 35.0%
!!! AGACGCCACTTTTGTAAAAT+TGG - Chr4:66525502-66525521 None:intergenic 35.0%
AAAACTCATGGGGAACTGAT+TGG + Chr4:66525612-66525631 MsG0480021964.01.T01:CDS 40.0%
AAACTCATGGGGAACTGATT+GGG + Chr4:66525613-66525632 MsG0480021964.01.T01:CDS 40.0%
AACATCCTCATACCCAGTAA+TGG - Chr4:66525396-66525415 None:intergenic 40.0%
AAGTTTGCCTTTCAAGCAGT+GGG - Chr4:66525719-66525738 None:intergenic 40.0%
ACTTTGTGGCATTGCAATGA+TGG + Chr4:66525685-66525704 MsG0480021964.01.T01:CDS 40.0%
CATTGACCTTGATTCTTCAC+GGG - Chr4:66525012-66525031 None:intergenic 40.0%
GACACAAAAGGTGAAGATCA+AGG + Chr4:66525236-66525255 MsG0480021964.01.T01:CDS 40.0%
GCATCAATGGCTACAGAAAT+TGG - Chr4:66525464-66525483 None:intergenic 40.0%
GGGGAGAAGAAGGATATATT+AGG + Chr4:66525633-66525652 MsG0480021964.01.T01:CDS 40.0%
GGGGTTCATAAGTTAAGTAC+TGG + Chr4:66525173-66525192 MsG0480021964.01.T01:CDS 40.0%
GGTTATGGCATTAGCAATGA+TGG + Chr4:66525569-66525588 MsG0480021964.01.T01:CDS 40.0%
GTGAAGAATCAAGGTCAATG+TGG + Chr4:66525012-66525031 MsG0480021964.01.T01:CDS 40.0%
GTGTTCCATCCACAATAGAT+TGG + Chr4:66524965-66524984 MsG0480021964.01.T01:CDS 40.0%
GTTCCATCAACACCTTGATA+GGG - Chr4:66525338-66525357 None:intergenic 40.0%
TGCCCAACAACATCCTATAA+AGG - Chr4:66525129-66525148 None:intergenic 40.0%
TGCTCGTGTTTCTCATACAT+GGG - Chr4:66524703-66524722 None:intergenic 40.0%
TGTATGAGAAACACGAGCAA+TGG + Chr4:66524704-66524723 MsG0480021964.01.T01:CDS 40.0%
TGTTCCATCAACACCTTGAT+AGG - Chr4:66525339-66525358 None:intergenic 40.0%
TTCCATCAACACCTTGATAG+GGG - Chr4:66525337-66525356 None:intergenic 40.0%
TTCCATCCACAATAGATTGG+AGG + Chr4:66524968-66524987 MsG0480021964.01.T01:CDS 40.0%
TTCCTCCAATCTATTGTGGA+TGG - Chr4:66524973-66524992 None:intergenic 40.0%
TTCTTATCCCACTGCTTGAA+AGG + Chr4:66525709-66525728 MsG0480021964.01.T01:exon 40.0%
TTGCTCGTGTTTCTCATACA+TGG - Chr4:66524704-66524723 None:intergenic 40.0%
! AATCAACCTGAAGCACTTGT+TGG + Chr4:66524534-66524553 MsG0480021964.01.T01:five_prime_UTR 40.0%
! GTGCCCCTTGAATCTATTTT+TGG - Chr4:66524896-66524915 None:intergenic 40.0%
!! AACACCTTGATAGGGGTATT+CGG - Chr4:66525330-66525349 None:intergenic 40.0%
!! GTATGGCTTGTTAGCATTGT+TGG - Chr4:66524821-66524840 None:intergenic 40.0%
AACTCATGGGGAACTGATTG+GGG + Chr4:66525614-66525633 MsG0480021964.01.T01:CDS 45.0%
AGGTGAAGATCAAGGTTGTG+AGG + Chr4:66525244-66525263 MsG0480021964.01.T01:CDS 45.0%
ATGTGAATTCCTCGTTGGTG+AGG - Chr4:66524870-66524889 None:intergenic 45.0%
CAAGTTTGCCTTTCAAGCAG+TGG - Chr4:66525720-66525739 None:intergenic 45.0%
CTTATGAACCCCTTCAGTTG+CGG - Chr4:66525165-66525184 None:intergenic 45.0%
GAAACCGAATACCCCTATCA+AGG + Chr4:66525323-66525342 MsG0480021964.01.T01:CDS 45.0%
GAGAAACACGAGCAATGGAT+GGG + Chr4:66524709-66524728 MsG0480021964.01.T01:CDS 45.0%
GCAAATGAAACTTCAACCCC+TGG + Chr4:66525365-66525384 MsG0480021964.01.T01:CDS 45.0%
GCTAACAAGCCATACAAGCT+TGG + Chr4:66524826-66524845 MsG0480021964.01.T01:CDS 45.0%
GCTCGTGTTTCTCATACATG+GGG - Chr4:66524702-66524721 None:intergenic 45.0%
GGATGATGTGAATTCCTCGT+TGG - Chr4:66524875-66524894 None:intergenic 45.0%
GGTGAAGATCAAGGTTGTGA+GGG + Chr4:66525245-66525264 MsG0480021964.01.T01:CDS 45.0%
GTAACACCCGTGAAGAATCA+AGG + Chr4:66525003-66525022 MsG0480021964.01.T01:CDS 45.0%
TACAAAAGTGGCGTCTTCAC+TGG + Chr4:66525506-66525525 MsG0480021964.01.T01:CDS 45.0%
TGAGAAACACGAGCAATGGA+TGG + Chr4:66524708-66524727 MsG0480021964.01.T01:CDS 45.0%
TGAGAACCAACAAGTGCTTC+AGG - Chr4:66524543-66524562 None:intergenic 45.0%
TGCTACCATTACTGGGTATG+AGG + Chr4:66525388-66525407 MsG0480021964.01.T01:CDS 45.0%
TGGGGTGGTCATCTAAATCT+TGG - Chr4:66524684-66524703 None:intergenic 45.0%
TGGTTCATGTGGAACTGAGT+TGG + Chr4:66525526-66525545 MsG0480021964.01.T01:CDS 45.0%
! TGTGGAACTGAGTTGGATCA+TGG + Chr4:66525533-66525552 MsG0480021964.01.T01:CDS 45.0%
!! GATCATGGTGTTACTGCTGT+GGG + Chr4:66525548-66525567 MsG0480021964.01.T01:CDS 45.0%
!! TACCCCTATCAAGGTGTTGA+TGG + Chr4:66525332-66525351 MsG0480021964.01.T01:CDS 45.0%
ACCCAGTAATGGTAGCACCA+GGG - Chr4:66525385-66525404 None:intergenic 50.0%
AGCTATTGCCGCAACTGAAG+GGG + Chr4:66525154-66525173 MsG0480021964.01.T01:CDS 50.0%
ATTTGCAGACCTCACCAACG+AGG + Chr4:66524858-66524877 MsG0480021964.01.T01:CDS 50.0%
CAGCTATTGCCGCAACTGAA+GGG + Chr4:66525153-66525172 MsG0480021964.01.T01:CDS 50.0%
CGTGTTTCTCATACATGGGG+TGG - Chr4:66524699-66524718 None:intergenic 50.0%
GAAGATCAAGGTTGTGAGGG+TGG + Chr4:66525248-66525267 MsG0480021964.01.T01:CDS 50.0%
GAGCAATGGATGGGTCACTA+TGG + Chr4:66524718-66524737 MsG0480021964.01.T01:CDS 50.0%
GATGCTGCTGAAGGACTTTG+TGG + Chr4:66525671-66525690 MsG0480021964.01.T01:CDS 50.0%
GGAACTGATTGGGGAGAAGA+AGG + Chr4:66525623-66525642 MsG0480021964.01.T01:CDS 50.0%
TACCCAGTAATGGTAGCACC+AGG - Chr4:66525386-66525405 None:intergenic 50.0%
TCAGCTATTGCCGCAACTGA+AGG + Chr4:66525152-66525171 MsG0480021964.01.T01:CDS 50.0%
TCTGTAGCCATTGATGCGAG+TGG + Chr4:66525467-66525486 MsG0480021964.01.T01:CDS 50.0%
!! GGATCATGGTGTTACTGCTG+TGG + Chr4:66525547-66525566 MsG0480021964.01.T01:CDS 50.0%
!! GGTGTTACTGCTGTGGGTTA+TGG + Chr4:66525554-66525573 MsG0480021964.01.T01:CDS 50.0%
AGCCATTGATGCGAGTGGCT+CGG + Chr4:66525472-66525491 MsG0480021964.01.T01:CDS 55.0%
ATCCGAGCCACTCGCATCAA+TGG - Chr4:66525477-66525496 None:intergenic 55.0%
CCCAGTAATGGTAGCACCAG+GGG - Chr4:66525384-66525403 None:intergenic 55.0%
! AGGGGTGTAGATGCTGCTGA+AGG + Chr4:66525662-66525681 MsG0480021964.01.T01:CDS 55.0%
! AGGTTGTGAGGGTGGCCTTA+TGG + Chr4:66525256-66525275 MsG0480021964.01.T01:CDS 55.0%
!! ACCCCTGGTGCTACCATTAC+TGG + Chr4:66525380-66525399 MsG0480021964.01.T01:CDS 55.0%
!! CCCCTGGTGCTACCATTACT+GGG + Chr4:66525381-66525400 MsG0480021964.01.T01:CDS 55.0%
!! GGCGTCTTCACTGGTTCATG+TGG + Chr4:66525515-66525534 MsG0480021964.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr4 gene 66524500 66525853 66524500 ID=MsG0480021964.01;Name=MsG0480021964.01
Chr4 mRNA 66524500 66525853 66524500 ID=MsG0480021964.01.T01;Parent=MsG0480021964.01;Name=MsG0480021964.01.T01;_AED=0.19;_eAED=0.19;_QI=89|1|1|1|1|1|2|126|345
Chr4 exon 66524500 66525033 66524500 ID=MsG0480021964.01.T01:exon:7343;Parent=MsG0480021964.01.T01
Chr4 exon 66525135 66525853 66525135 ID=MsG0480021964.01.T01:exon:7344;Parent=MsG0480021964.01.T01
Chr4 five_prime_UTR 66524500 66524588 66524500 ID=MsG0480021964.01.T01:five_prime_utr;Parent=MsG0480021964.01.T01
Chr4 CDS 66524589 66525033 66524589 ID=MsG0480021964.01.T01:cds;Parent=MsG0480021964.01.T01
Chr4 CDS 66525135 66525727 66525135 ID=MsG0480021964.01.T01:cds;Parent=MsG0480021964.01.T01
Chr4 three_prime_UTR 66525728 66525853 66525728 ID=MsG0480021964.01.T01:three_prime_utr;Parent=MsG0480021964.01.T01
Gene Sequence

>MsG0480021964.01.T01

ATGACTACAAATAATCAATTATATCATAGCATTTCATTGACATTGCTTTTATGCTTGGGATTGTTTGCTATTCAAGTCACTTCTCGTACTCTCCAAGATTTAGATGACCACCCCATGTATGAGAAACACGAGCAATGGATGGGTCACTATGGCAAAGTATATAAGGATCTTCAAGAAAGAGAGAATCGTTTAAAGATATTCAAAGAAAATGTGAATTACATTGAAGCTTCCAACAATGCTAACAAGCCATACAAGCTTGGCATTAATCAATTTGCAGACCTCACCAACGAGGAATTCACATCATCCAAAAATAGATTCAAGGGGCACATGTGTTCTTCTATCACTAGGACAGCTACTTTTAAATATGAAAATGCGAGTGTTCCATCCACAATAGATTGGAGGAAGAAAGGAGCAGTAACACCCGTGAAGAATCAAGGTCAATGTGGATGTTGTTGGGCATTCTCAGCTATTGCCGCAACTGAAGGGGTTCATAAGTTAAGTACTGGAAAACTGATCTCTTTATCAGAACAAGAACTTGTTGATTGTGACACAAAAGGTGAAGATCAAGGTTGTGAGGGTGGCCTTATGGATGATGCTTTCAAATTCATCATTCAAAATAATGGAATAAGCACAGAAACCGAATACCCCTATCAAGGTGTTGATGGAACATGCAATGCAAATGAAACTTCAACCCCTGGTGCTACCATTACTGGGTATGAGGATGTTCCAACAAACAATGAGAATGCACTGCAAAAAGCTGTAGCTAATCAACCAATTTCTGTAGCCATTGATGCGAGTGGCTCGGATTTCCAATTTTACAAAAGTGGCGTCTTCACTGGTTCATGTGGAACTGAGTTGGATCATGGTGTTACTGCTGTGGGTTATGGCATTAGCAATGATGGAACCAAATATTGGTTGGTTAAAAACTCATGGGGAACTGATTGGGGAGAAGAAGGATATATTAGGATGCAAAGGGGTGTAGATGCTGCTGAAGGACTTTGTGGCATTGCAATGATGGCTTCTTATCCCACTGCTTGA
Protein sequence

>MsG0480021964.01.T01

MTTNNQLYHSISLTLLLCLGLFAIQVTSRTLQDLDDHPMYEKHEQWMGHYGKVYKDLQERENRLKIFKENVNYIEASNNANKPYKLGINQFADLTNEEFTSSKNRFKGHMCSSITRTATFKYENASVPSTIDWRKKGAVTPVKNQGQCGCCWAFSAIAATEGVHKLSTGKLISLSEQELVDCDTKGEDQGCEGGLMDDAFKFIIQNNGISTETEYPYQGVDGTCNANETSTPGATITGYEDVPTNNENALQKAVANQPISVAIDASGSDFQFYKSGVFTGSCGTELDHGVTAVGYGISNDGTKYWLVKNSWGTDWGEEGYIRMQRGVDAAEGLCGIAMMASYPTA*