Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021972.01.T01 | XP_003607586.1 | 97.727 | 176 | 4 | 0 | 1 | 176 | 1 | 176 | 5.80E-126 | 361 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021972.01.T01 | Q9S7R3 | 84.615 | 143 | 20 | 2 | 10 | 152 | 8 | 148 | 3.04E-87 | 256 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021972.01.T01 | G7JCL4 | 97.727 | 176 | 4 | 0 | 1 | 176 | 1 | 176 | 2.77e-126 | 361 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048633.01 | MsG0480021972.01 | 0.801525 | 8.643514e-49 | 4.136862e-46 |
| MsG0080048652.01 | MsG0480021972.01 | 0.826545 | 2.557619e-54 | 2.405831e-51 |
| MsG0180004559.01 | MsG0480021972.01 | 0.828882 | 7.022461e-55 | 7.069983e-52 |
| MsG0480021972.01 | MsG0480023195.01 | 0.815686 | 8.139064e-52 | 5.650077e-49 |
| MsG0480021972.01 | MsG0580029623.01 | 0.803335 | 3.662106e-49 | 1.835363e-46 |
| MsG0480021972.01 | MsG0880043261.01 | 0.832069 | 1.166769e-55 | 1.289968e-52 |
| MsG0480021972.01 | MsG0880043983.01 | 0.812159 | 4.878310e-51 | 3.078517e-48 |
| MsG0380017231.01 | MsG0480021972.01 | 0.811541 | 6.649142e-51 | 4.127139e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021972.01.T01 | MTR_4g079930 | 97.727 | 176 | 4 | 0 | 1 | 176 | 1 | 176 | 7.02e-130 | 361 |
| MsG0480021972.01.T01 | MTR_2g092950 | 83.832 | 167 | 23 | 3 | 10 | 176 | 5 | 167 | 3.32e-98 | 281 |
| MsG0480021972.01.T01 | MTR_2g016110 | 76.471 | 153 | 36 | 0 | 21 | 173 | 27 | 179 | 3.13e-86 | 251 |
| MsG0480021972.01.T01 | MTR_2g076400 | 80.405 | 148 | 29 | 0 | 27 | 174 | 31 | 178 | 3.59e-85 | 248 |
| MsG0480021972.01.T01 | MTR_5g072510 | 65.972 | 144 | 47 | 1 | 14 | 157 | 24 | 165 | 6.56e-73 | 219 |
| MsG0480021972.01.T01 | MTR_4g094428 | 71.756 | 131 | 37 | 0 | 27 | 157 | 44 | 174 | 1.09e-71 | 216 |
| MsG0480021972.01.T01 | MTR_1g442860 | 74.400 | 125 | 32 | 0 | 29 | 153 | 65 | 189 | 5.97e-70 | 211 |
| MsG0480021972.01.T01 | MTR_8g086350 | 82.540 | 126 | 22 | 0 | 27 | 152 | 29 | 154 | 6.83e-70 | 210 |
| MsG0480021972.01.T01 | MTR_1g075990 | 72.794 | 136 | 37 | 0 | 22 | 157 | 46 | 181 | 8.21e-66 | 200 |
| MsG0480021972.01.T01 | MTR_3g031830 | 63.924 | 158 | 43 | 1 | 14 | 157 | 13 | 170 | 6.63e-64 | 196 |
| MsG0480021972.01.T01 | MTR_7g115700 | 62.162 | 148 | 51 | 3 | 27 | 171 | 25 | 170 | 1.05e-63 | 194 |
| MsG0480021972.01.T01 | MTR_7g097030 | 74.615 | 130 | 33 | 0 | 28 | 157 | 63 | 192 | 5.11e-63 | 193 |
| MsG0480021972.01.T01 | MTR_1g069825 | 74.419 | 129 | 33 | 0 | 29 | 157 | 52 | 180 | 2.35e-62 | 192 |
| MsG0480021972.01.T01 | MTR_1g080210 | 66.434 | 143 | 48 | 0 | 29 | 171 | 40 | 182 | 2.74e-58 | 180 |
| MsG0480021972.01.T01 | MTR_1g114020 | 66.667 | 45 | 15 | 0 | 28 | 72 | 70 | 114 | 1.06e-14 | 68.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021972.01.T01 | AT2G42610 | 84.615 | 143 | 20 | 2 | 10 | 152 | 8 | 148 | 3.10e-88 | 256 |
| MsG0480021972.01.T01 | AT2G42610 | 84.615 | 143 | 20 | 2 | 10 | 152 | 8 | 148 | 3.10e-88 | 256 |
| MsG0480021972.01.T01 | AT1G07090 | 65.385 | 156 | 54 | 0 | 2 | 157 | 4 | 159 | 1.15e-76 | 228 |
| MsG0480021972.01.T01 | AT1G78815 | 76.378 | 127 | 30 | 0 | 26 | 152 | 37 | 163 | 3.20e-75 | 224 |
| MsG0480021972.01.T01 | AT4G18610 | 80.159 | 126 | 24 | 1 | 28 | 152 | 36 | 161 | 1.19e-74 | 222 |
| MsG0480021972.01.T01 | AT1G16910 | 68.531 | 143 | 45 | 0 | 23 | 165 | 17 | 159 | 4.14e-72 | 215 |
| MsG0480021972.01.T01 | AT5G58500 | 70.992 | 131 | 37 | 1 | 28 | 157 | 18 | 148 | 2.48e-70 | 211 |
| MsG0480021972.01.T01 | AT3G23290 | 76.378 | 127 | 30 | 0 | 27 | 153 | 46 | 172 | 5.97e-64 | 195 |
| MsG0480021972.01.T01 | AT2G31160 | 73.643 | 129 | 34 | 0 | 29 | 157 | 54 | 182 | 1.12e-63 | 195 |
| MsG0480021972.01.T01 | AT5G28490 | 65.806 | 155 | 52 | 1 | 9 | 163 | 6 | 159 | 1.52e-63 | 194 |
| MsG0480021972.01.T01 | AT3G04510 | 71.212 | 132 | 38 | 0 | 29 | 160 | 33 | 164 | 8.89e-63 | 192 |
Find 43 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCGTTACTTGGACCAATT+TGG | 0.191736 | 4:+66603703 | MsG0480021972.01.T01:CDS |
| GGAAGCTTAGATGCTCTTAT+TGG | 0.259171 | 4:+66603814 | MsG0480021972.01.T01:CDS |
| AGAAGAGATTGGAACACTTT+TGG | 0.305989 | 4:+66603610 | MsG0480021972.01.T01:CDS |
| GTTCCATTCGTGTCTACCTT+AGG | 0.324117 | 4:+66603899 | MsG0480021972.01.T01:CDS |
| ATGCTCTTATTGGAAGGTTA+AGG | 0.327795 | 4:+66603824 | MsG0480021972.01.T01:CDS |
| TCAAGAAAATTGAATATTGC+TGG | 0.334695 | 4:-66604016 | None:intergenic |
| GTACAAGGTGCAGGTGGTTC+TGG | 0.337886 | 4:-66603769 | None:intergenic |
| CTAGCAAAAGGATTAGTATC+AGG | 0.342280 | 4:-66603874 | None:intergenic |
| TGCTCTTATTGGAAGGTTAA+GGG | 0.358240 | 4:+66603825 | MsG0480021972.01.T01:CDS |
| TTGTCCTCTTAGACAAGCTT+GGG | 0.372306 | 4:+66603792 | MsG0480021972.01.T01:CDS |
| TTTAGATTTCCTTCGTTACT+TGG | 0.382958 | 4:+66603693 | MsG0480021972.01.T01:CDS |
| CTTCCCCAAGCTTGTCTAAG+AGG | 0.435738 | 4:-66603796 | None:intergenic |
| AATCACAAAAGAGAAGAGAT+TGG | 0.447023 | 4:+66603599 | MsG0480021972.01.T01:CDS |
| AGGGCTGCTTATGAAGAAAA+TGG | 0.451783 | 4:+66603844 | MsG0480021972.01.T01:CDS |
| CTTGTCCTCTTAGACAAGCT+TGG | 0.466135 | 4:+66603791 | MsG0480021972.01.T01:CDS |
| TTCCATTCGTGTCTACCTTA+GGG | 0.466186 | 4:+66603900 | MsG0480021972.01.T01:CDS |
| AAATTGAATATTGCTGGAGC+TGG | 0.466512 | 4:-66604010 | None:intergenic |
| TTGCACTGTGAAAGTGGAAC+TGG | 0.487945 | 4:-66603658 | None:intergenic |
| AAAACTAAGGTTCATTTACA+AGG | 0.499878 | 4:+66603727 | MsG0480021972.01.T01:CDS |
| GCTAAGTGTCGCTACGGGAG+TGG | 0.514607 | 4:-66603569 | None:intergenic |
| CGAATGGAACCACTAGCAAA+AGG | 0.517835 | 4:-66603886 | None:intergenic |
| GATTAGAGAGTCACAAGCTA+AGG | 0.523586 | 4:+66603924 | MsG0480021972.01.T01:CDS |
| GAAAAGCACAAGTCAAAGCA+AGG | 0.536094 | 4:+66603975 | MsG0480021972.01.T01:CDS |
| GCTTAGATGCTCTTATTGGA+AGG | 0.540733 | 4:+66603818 | MsG0480021972.01.T01:CDS |
| AAAAGCACAAGTCAAAGCAA+GGG | 0.546373 | 4:+66603976 | MsG0480021972.01.T01:CDS |
| TAGCAAAAGGATTAGTATCA+GGG | 0.547090 | 4:-66603873 | None:intergenic |
| ATAGCGGCTAAGTGTCGCTA+CGG | 0.563998 | 4:-66603575 | None:intergenic |
| TTGCTATTGCACTGTGAAAG+TGG | 0.566505 | 4:-66603664 | None:intergenic |
| AGAGGACAAGTACAAGGTGC+AGG | 0.577150 | 4:-66603778 | None:intergenic |
| CTCCCTAAGGTAGACACGAA+TGG | 0.577313 | 4:-66603902 | None:intergenic |
| GAGTCACAAGCTAAGGCTAG+AGG | 0.580960 | 4:+66603931 | MsG0480021972.01.T01:CDS |
| GTGACTCTCTAATCTCCCTA+AGG | 0.581923 | 4:-66603915 | None:intergenic |
| TGTCTAAGAGGACAAGTACA+AGG | 0.604570 | 4:-66603784 | None:intergenic |
| TAGCGGCTAAGTGTCGCTAC+GGG | 0.605733 | 4:-66603574 | None:intergenic |
| CAAATTGGTCCAAGTAACGA+AGG | 0.614807 | 4:-66603702 | None:intergenic |
| CACTGTGAAAGTGGAACTGG+TGG | 0.616068 | 4:-66603655 | None:intergenic |
| CACAAGAAAATGTCTACTAG+TGG | 0.633092 | 4:+66603499 | None:intergenic |
| CTGCTTATGAAGAAAATGGT+GGG | 0.645013 | 4:+66603848 | MsG0480021972.01.T01:CDS |
| AGCAAAAGGATTAGTATCAG+GGG | 0.648452 | 4:-66603872 | None:intergenic |
| TGTCCTCTTAGACAAGCTTG+GGG | 0.649148 | 4:+66603793 | MsG0480021972.01.T01:CDS |
| GCTGCTTATGAAGAAAATGG+TGG | 0.659838 | 4:+66603847 | MsG0480021972.01.T01:CDS |
| AACGAAGGAAATCTAAAACA+TGG | 0.665632 | 4:-66603687 | None:intergenic |
| GGACAAGTACAAGGTGCAGG+TGG | 0.672826 | 4:-66603775 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAACTAAGGTTCATTTACA+AGG | + | Chr4:66603727-66603746 | MsG0480021972.01.T01:CDS | 25.0% |
| ! | TCAAGAAAATTGAATATTGC+TGG | - | Chr4:66604019-66604038 | None:intergenic | 25.0% |
| !! | CTTTTCTTTTTCTTCTTGTA+TGG | - | Chr4:66603961-66603980 | None:intergenic | 25.0% |
| !! | TTACAAGGTTGTATGTTTTA+TGG | + | Chr4:66603742-66603761 | MsG0480021972.01.T01:CDS | 25.0% |
| AATCACAAAAGAGAAGAGAT+TGG | + | Chr4:66603599-66603618 | MsG0480021972.01.T01:CDS | 30.0% | |
| TTTAGATTTCCTTCGTTACT+TGG | + | Chr4:66603693-66603712 | MsG0480021972.01.T01:CDS | 30.0% | |
| ! | AACGAAGGAAATCTAAAACA+TGG | - | Chr4:66603690-66603709 | None:intergenic | 30.0% |
| ! | TAGCAAAAGGATTAGTATCA+GGG | - | Chr4:66603876-66603895 | None:intergenic | 30.0% |
| ! | TTCTCTTTTGTGATTCATAG+CGG | - | Chr4:66603594-66603613 | None:intergenic | 30.0% |
| !! | GAACCTTAGTTTTTCCAAAT+TGG | - | Chr4:66603720-66603739 | None:intergenic | 30.0% |
| AAATTGAATATTGCTGGAGC+TGG | - | Chr4:66604013-66604032 | None:intergenic | 35.0% | |
| ATGCTCTTATTGGAAGGTTA+AGG | + | Chr4:66603824-66603843 | MsG0480021972.01.T01:CDS | 35.0% | |
| CTGCTTATGAAGAAAATGGT+GGG | + | Chr4:66603848-66603867 | MsG0480021972.01.T01:CDS | 35.0% | |
| GGACCAATTTGGAAAAACTA+AGG | + | Chr4:66603714-66603733 | MsG0480021972.01.T01:CDS | 35.0% | |
| TGCTCTTATTGGAAGGTTAA+GGG | + | Chr4:66603825-66603844 | MsG0480021972.01.T01:CDS | 35.0% | |
| ! | AAAAGCACAAGTCAAAGCAA+GGG | + | Chr4:66603976-66603995 | MsG0480021972.01.T01:CDS | 35.0% |
| ! | ACTAGTGGTAGCAATTTTCA+TGG | + | Chr4:66603514-66603533 | MsG0480021972.01.T01:CDS | 35.0% |
| ! | AGAAGAGATTGGAACACTTT+TGG | + | Chr4:66603610-66603629 | MsG0480021972.01.T01:CDS | 35.0% |
| ! | AGCAAAAGGATTAGTATCAG+GGG | - | Chr4:66603875-66603894 | None:intergenic | 35.0% |
| ! | CAATTTTCATGGCAAGAATC+TGG | + | Chr4:66603525-66603544 | MsG0480021972.01.T01:CDS | 35.0% |
| ! | CTAGCAAAAGGATTAGTATC+AGG | - | Chr4:66603877-66603896 | None:intergenic | 35.0% |
| ! | GATACTAATCCTTTTGCTAG+TGG | + | Chr4:66603877-66603896 | MsG0480021972.01.T01:CDS | 35.0% |
| AGGGCTGCTTATGAAGAAAA+TGG | + | Chr4:66603844-66603863 | MsG0480021972.01.T01:CDS | 40.0% | |
| CAAATTGGTCCAAGTAACGA+AGG | - | Chr4:66603705-66603724 | None:intergenic | 40.0% | |
| CTTCGTTACTTGGACCAATT+TGG | + | Chr4:66603703-66603722 | MsG0480021972.01.T01:CDS | 40.0% | |
| GATTAGAGAGTCACAAGCTA+AGG | + | Chr4:66603924-66603943 | MsG0480021972.01.T01:CDS | 40.0% | |
| GCTGCTTATGAAGAAAATGG+TGG | + | Chr4:66603847-66603866 | MsG0480021972.01.T01:CDS | 40.0% | |
| GCTTAGATGCTCTTATTGGA+AGG | + | Chr4:66603818-66603837 | MsG0480021972.01.T01:CDS | 40.0% | |
| GGAAGCTTAGATGCTCTTAT+TGG | + | Chr4:66603814-66603833 | MsG0480021972.01.T01:CDS | 40.0% | |
| TGTCTAAGAGGACAAGTACA+AGG | - | Chr4:66603787-66603806 | None:intergenic | 40.0% | |
| TTCCATTCGTGTCTACCTTA+GGG | + | Chr4:66603900-66603919 | MsG0480021972.01.T01:CDS | 40.0% | |
| TTGTCCTCTTAGACAAGCTT+GGG | + | Chr4:66603792-66603811 | MsG0480021972.01.T01:CDS | 40.0% | |
| ! | GAAAAGCACAAGTCAAAGCA+AGG | + | Chr4:66603975-66603994 | MsG0480021972.01.T01:CDS | 40.0% |
| ! | TTGCTATTGCACTGTGAAAG+TGG | - | Chr4:66603667-66603686 | None:intergenic | 40.0% |
| CGAATGGAACCACTAGCAAA+AGG | - | Chr4:66603889-66603908 | None:intergenic | 45.0% | |
| CTTGTCCTCTTAGACAAGCT+TGG | + | Chr4:66603791-66603810 | MsG0480021972.01.T01:CDS | 45.0% | |
| GTGACTCTCTAATCTCCCTA+AGG | - | Chr4:66603918-66603937 | None:intergenic | 45.0% | |
| GTTCCATTCGTGTCTACCTT+AGG | + | Chr4:66603899-66603918 | MsG0480021972.01.T01:CDS | 45.0% | |
| TGTCCTCTTAGACAAGCTTG+GGG | + | Chr4:66603793-66603812 | MsG0480021972.01.T01:CDS | 45.0% | |
| TTGCACTGTGAAAGTGGAAC+TGG | - | Chr4:66603661-66603680 | None:intergenic | 45.0% | |
| AGAGGACAAGTACAAGGTGC+AGG | - | Chr4:66603781-66603800 | None:intergenic | 50.0% | |
| CACTGTGAAAGTGGAACTGG+TGG | - | Chr4:66603658-66603677 | None:intergenic | 50.0% | |
| CTCCCTAAGGTAGACACGAA+TGG | - | Chr4:66603905-66603924 | None:intergenic | 50.0% | |
| CTTCCCCAAGCTTGTCTAAG+AGG | - | Chr4:66603799-66603818 | None:intergenic | 50.0% | |
| GAGTCACAAGCTAAGGCTAG+AGG | + | Chr4:66603931-66603950 | MsG0480021972.01.T01:CDS | 50.0% | |
| ! | ATAGCGGCTAAGTGTCGCTA+CGG | - | Chr4:66603578-66603597 | None:intergenic | 50.0% |
| GGACAAGTACAAGGTGCAGG+TGG | - | Chr4:66603778-66603797 | None:intergenic | 55.0% | |
| GTACAAGGTGCAGGTGGTTC+TGG | - | Chr4:66603772-66603791 | None:intergenic | 55.0% | |
| ! | TAGCGGCTAAGTGTCGCTAC+GGG | - | Chr4:66603577-66603596 | None:intergenic | 55.0% |
| ! | GCTAAGTGTCGCTACGGGAG+TGG | - | Chr4:66603572-66603591 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 66603508 | 66604038 | 66603508 | ID=MsG0480021972.01;Name=MsG0480021972.01 |
| Chr4 | mRNA | 66603508 | 66604038 | 66603508 | ID=MsG0480021972.01.T01;Parent=MsG0480021972.01;Name=MsG0480021972.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|176 |
| Chr4 | exon | 66603508 | 66604038 | 66603508 | ID=MsG0480021972.01.T01:exon:8083;Parent=MsG0480021972.01.T01 |
| Chr4 | CDS | 66603508 | 66604038 | 66603508 | ID=MsG0480021972.01.T01:cds;Parent=MsG0480021972.01.T01 |
| Gene Sequence |
| Protein sequence |
