AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017231.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017231.01.T01 MTR_3g103330 94.444 108 2 1 1 108 1 104 1.09e-69 203
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTCGAGTCAAAGCTCTTC+TGG 0.318499 3:+93822602 MsG0380017231.01.T01:CDS
CGCCATGACGGTGGTGTAGC+TGG 0.367268 3:+93822785 MsG0380017231.01.T01:CDS
GGTTGCAGTGGAAAGCTTGA+TGG 0.410694 3:+93822623 MsG0380017231.01.T01:CDS
TTTACCGGTCCTTCTCCTGC+TGG 0.444782 3:-93822813 None:intergenic
GCAGGAGAAGGACCGGTAAA+GGG 0.444911 3:+93822816 MsG0380017231.01.T01:CDS
AGCAGGAGAAGGACCGGTAA+AGG 0.449501 3:+93822815 MsG0380017231.01.T01:CDS
GAAACCACTGCAGGTGGAAG+AGG 0.474000 3:-93822555 None:intergenic
TTCATCTAGATCATTGTTCG+TGG 0.475117 3:-93822856 None:intergenic
GAACATCAATGGCAACTCAT+TGG 0.481610 3:-93822747 None:intergenic
GTTGATCAATGGTGTCACTA+TGG 0.489333 3:+93822658 MsG0380017231.01.T01:CDS
GTGTAGCTGGTTCCACCAGC+AGG 0.495334 3:+93822798 MsG0380017231.01.T01:CDS
ACCGGTCCTTCTCCTGCTGG+TGG 0.523534 3:-93822810 None:intergenic
TTACCATCGTTGAACATCAA+TGG 0.525954 3:-93822758 None:intergenic
ATCTTCTCCATCTTCTTCTG+TGG 0.543326 3:-93822723 None:intergenic
ACTACCTCTTCCACCTGCAG+TGG 0.560557 3:+93822551 MsG0380017231.01.T01:CDS
CAAAGCTCTTCTGGTTGCAG+TGG 0.566167 3:+93822611 MsG0380017231.01.T01:CDS
TCCACCAGCAGGAGAAGGAC+CGG 0.569119 3:+93822809 MsG0380017231.01.T01:CDS
CGCATCGCCACAGAAGAAGA+TGG 0.574444 3:+93822716 MsG0380017231.01.T01:CDS
CTGGTTCCACCAGCAGGAGA+AGG 0.578852 3:+93822804 MsG0380017231.01.T01:CDS
TCGAAGTATTCGATGATGAT+CGG 0.586920 3:-93822586 None:intergenic
GTAGCTATGTGGTTGATCAA+TGG 0.596285 3:+93822647 MsG0380017231.01.T01:CDS
GAAGTTGAAGAAACCACTGC+AGG 0.609331 3:-93822564 None:intergenic
GTTGAAGAAACCACTGCAGG+TGG 0.625992 3:-93822561 None:intergenic
GGTAATCTCCGCCATGACGG+TGG 0.628227 3:+93822776 MsG0380017231.01.T01:CDS
AACCAGCTACACCACCGTCA+TGG 0.629069 3:-93822787 None:intergenic
GATGGTAATCTCCGCCATGA+CGG 0.678032 3:+93822773 MsG0380017231.01.T01:CDS
AGCTTGATGGAGTAGCTATG+TGG 0.699449 3:+93822636 MsG0380017231.01.T01:CDS
CAGCTACACCACCGTCATGG+CGG 0.723788 3:-93822784 None:intergenic
TTGCCATTGATGTTCAACGA+TGG 0.744804 3:+93822755 MsG0380017231.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TTACCATCGTTGAACATCAA+TGG - Chr3:93822761-93822780 None:intergenic 35.0%
! TACACTTTTCTTCCCTTTAC+CGG - Chr3:93822831-93822850 None:intergenic 35.0%
! TCGAAGTATTCGATGATGAT+CGG - Chr3:93822589-93822608 None:intergenic 35.0%
ATCTTCTCCATCTTCTTCTG+TGG - Chr3:93822726-93822745 None:intergenic 40.0%
GAACATCAATGGCAACTCAT+TGG - Chr3:93822750-93822769 None:intergenic 40.0%
GTAGCTATGTGGTTGATCAA+TGG + Chr3:93822647-93822666 MsG0380017231.01.T01:CDS 40.0%
TTGCCATTGATGTTCAACGA+TGG + Chr3:93822755-93822774 MsG0380017231.01.T01:CDS 40.0%
!! GTTGATCAATGGTGTCACTA+TGG + Chr3:93822658-93822677 MsG0380017231.01.T01:CDS 40.0%
ACTTCGAGTCAAAGCTCTTC+TGG + Chr3:93822602-93822621 MsG0380017231.01.T01:CDS 45.0%
AGCTTGATGGAGTAGCTATG+TGG + Chr3:93822636-93822655 MsG0380017231.01.T01:CDS 45.0%
GAAGTTGAAGAAACCACTGC+AGG - Chr3:93822567-93822586 None:intergenic 45.0%
GATGGTAATCTCCGCCATGA+CGG + Chr3:93822773-93822792 MsG0380017231.01.T01:CDS 50.0%
GGTTGCAGTGGAAAGCTTGA+TGG + Chr3:93822623-93822642 MsG0380017231.01.T01:CDS 50.0%
GTTGAAGAAACCACTGCAGG+TGG - Chr3:93822564-93822583 None:intergenic 50.0%
! CAAAGCTCTTCTGGTTGCAG+TGG + Chr3:93822611-93822630 MsG0380017231.01.T01:CDS 50.0%
AACCAGCTACACCACCGTCA+TGG - Chr3:93822790-93822809 None:intergenic 55.0%
ACTACCTCTTCCACCTGCAG+TGG + Chr3:93822551-93822570 MsG0380017231.01.T01:CDS 55.0%
AGCAGGAGAAGGACCGGTAA+AGG + Chr3:93822815-93822834 MsG0380017231.01.T01:CDS 55.0%
CGCATCGCCACAGAAGAAGA+TGG + Chr3:93822716-93822735 MsG0380017231.01.T01:CDS 55.0%
GAAACCACTGCAGGTGGAAG+AGG - Chr3:93822558-93822577 None:intergenic 55.0%
GCAGGAGAAGGACCGGTAAA+GGG + Chr3:93822816-93822835 MsG0380017231.01.T01:CDS 55.0%
TTTACCGGTCCTTCTCCTGC+TGG - Chr3:93822816-93822835 None:intergenic 55.0%
CAGCTACACCACCGTCATGG+CGG - Chr3:93822787-93822806 None:intergenic 60.0%
CTGGTTCCACCAGCAGGAGA+AGG + Chr3:93822804-93822823 MsG0380017231.01.T01:CDS 60.0%
GGTAATCTCCGCCATGACGG+TGG + Chr3:93822776-93822795 MsG0380017231.01.T01:CDS 60.0%
TCCACCAGCAGGAGAAGGAC+CGG + Chr3:93822809-93822828 MsG0380017231.01.T01:CDS 60.0%
! GTGTAGCTGGTTCCACCAGC+AGG + Chr3:93822798-93822817 MsG0380017231.01.T01:CDS 60.0%
ACCGGTCCTTCTCCTGCTGG+TGG - Chr3:93822813-93822832 None:intergenic 65.0%
CGCCATGACGGTGGTGTAGC+TGG + Chr3:93822785-93822804 MsG0380017231.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 93822542 93822868 93822542 ID=MsG0380017231.01;Name=MsG0380017231.01
Chr3 mRNA 93822542 93822868 93822542 ID=MsG0380017231.01.T01;Parent=MsG0380017231.01;Name=MsG0380017231.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|108
Chr3 exon 93822542 93822868 93822542 ID=MsG0380017231.01.T01:exon:1442;Parent=MsG0380017231.01.T01
Chr3 CDS 93822542 93822868 93822542 ID=MsG0380017231.01.T01:cds;Parent=MsG0380017231.01.T01
Gene Sequence

>MsG0380017231.01.T01

ATGGCAGCTACTACCTCTTCCACCTGCAGTGGTTTCTTCAACTTCCGATCATCATCGAATACTTCGAGTCAAAGCTCTTCTGGTTGCAGTGGAAAGCTTGATGGAGTAGCTATGTGGTTGATCAATGGTGTCACTATGGCTTTTTTTTCATCTTTAAATCGTTGCTCTTGCATTCGCATCGCCACAGAAGAAGATGGAGAAGATGCCAATGAGTTGCCATTGATGTTCAACGATGGTAATCTCCGCCATGACGGTGGTGTAGCTGGTTCCACCAGCAGGAGAAGGACCGGTAAAGGGAAGAAAAGTGTACTTGTCCACGAACAATGA

Protein sequence

>MsG0380017231.01.T01

MAATTSSTCSGFFNFRSSSNTSSQSSSGCSGKLDGVAMWLINGVTMAFFSSLNRCSCIRIATEEDGEDANELPLMFNDGNLRHDGGVAGSTSRRRTGKGKKSVLVHEQ*