AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880043983.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043983.01.T01 MTR_8g045520 93.631 157 10 0 1 157 1 157 2.28e-108 305
MsG0880043983.01.T01 MTR_8g045490 80.255 157 30 1 1 157 1 156 3.73e-91 262
MsG0880043983.01.T01 MTR_8g045695 79.618 157 31 1 1 157 1 156 9.37e-90 258
MsG0880043983.01.T01 MTR_8g045400 78.981 157 32 1 1 157 1 156 1.18e-89 258
MsG0880043983.01.T01 MTR_8g045555 76.159 151 36 0 1 151 1 151 2.68e-86 249
MsG0880043983.01.T01 MTR_8g045300 75.000 156 39 0 1 156 1 156 9.91e-86 248
MsG0880043983.01.T01 MTR_8g045665 76.433 157 36 1 1 157 1 156 4.55e-85 246
MsG0880043983.01.T01 MTR_8g045570 75.497 151 37 0 1 151 1 151 1.05e-84 245
MsG0880043983.01.T01 MTR_8g045735 74.839 155 38 1 1 155 1 154 6.83e-84 243
MsG0880043983.01.T01 MTR_8g045640 75.159 157 38 1 1 157 1 156 7.21e-84 243
MsG0880043983.01.T01 MTR_8g045560 72.611 157 42 1 1 157 1 156 1.37e-80 235
MsG0880043983.01.T01 MTR_1g030810 42.208 154 86 2 1 152 1 153 4.25e-39 130
MsG0880043983.01.T01 MTR_1g030820 38.312 154 92 2 1 152 1 153 1.15e-37 126
MsG0880043983.01.T01 MTR_1g030840 36.538 156 91 4 1 149 1 155 4.85e-28 102
MsG0880043983.01.T01 MTR_8g012550 32.667 150 98 3 6 154 8 155 1.68e-26 98.2
MsG0880043983.01.T01 MTR_1g031640 35.374 147 86 5 7 151 7 146 3.19e-24 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880043983.01.T01 AT5G28010 34.211 152 97 3 3 152 15 165 4.77e-27 99.8
MsG0880043983.01.T01 AT5G28010 34.211 152 97 3 3 152 15 165 4.77e-27 99.8
MsG0880043983.01.T01 AT1G70890 36.184 152 94 3 3 152 7 157 1.10e-26 98.6
MsG0880043983.01.T01 AT1G23120 33.333 144 91 3 10 153 10 148 9.40e-25 93.6
MsG0880043983.01.T01 AT1G70840 32.895 152 99 3 3 152 20 170 2.07e-24 93.2
MsG0880043983.01.T01 AT1G23120 33.333 144 91 3 10 153 60 198 4.02e-24 93.2
MsG0880043983.01.T01 AT1G70830 33.553 152 98 3 3 152 22 172 7.81e-24 91.7
MsG0880043983.01.T01 AT1G70830 33.553 152 98 3 3 152 22 172 1.55e-23 91.7
MsG0880043983.01.T01 AT1G70870 37.748 151 81 4 1 151 1 138 2.02e-23 89.7
MsG0880043983.01.T01 AT5G28000 31.579 152 101 3 3 152 13 163 4.48e-23 89.7
MsG0880043983.01.T01 AT1G70830 33.553 152 98 3 3 152 22 172 5.74e-23 91.3
MsG0880043983.01.T01 AT1G70830 33.553 152 98 3 3 152 137 287 1.28e-22 91.3
MsG0880043983.01.T01 AT1G70830 28.421 95 67 1 59 152 31 125 3.39e-11 60.1
MsG0880043983.01.T01 AT1G70830 33.553 152 98 3 3 152 22 172 1.59e-22 91.7
MsG0880043983.01.T01 AT1G70830 33.553 152 98 3 3 152 184 334 3.90e-22 90.9
MsG0880043983.01.T01 AT1G70850 33.113 151 98 3 3 151 9 158 1.91e-22 89.7
MsG0880043983.01.T01 AT1G70850 33.113 151 98 3 3 151 9 158 5.35e-22 90.1
MsG0880043983.01.T01 AT1G70850 33.803 142 91 3 13 152 175 315 6.92e-22 89.7
MsG0880043983.01.T01 AT1G70850 33.113 151 98 3 3 151 9 158 5.35e-22 90.1
MsG0880043983.01.T01 AT1G70850 33.803 142 91 3 13 152 175 315 6.92e-22 89.7
MsG0880043983.01.T01 AT1G24020 33.117 154 99 3 1 152 1 152 1.86e-19 80.1
MsG0880043983.01.T01 AT1G24020 33.117 154 99 3 1 152 1 152 1.86e-19 80.1
MsG0880043983.01.T01 AT1G23130 27.152 151 108 2 3 152 10 159 6.14e-19 79.0
MsG0880043983.01.T01 AT1G70880 28.289 152 106 3 3 152 8 158 1.00e-18 78.2
MsG0880043983.01.T01 AT4G23670 34.194 155 93 4 1 151 1 150 1.15e-18 77.8
MsG0880043983.01.T01 AT1G14930 30.263 152 103 3 1 151 1 150 1.47e-17 75.1
MsG0880043983.01.T01 AT1G35260 28.082 146 101 3 9 152 8 151 4.00e-16 71.2
MsG0880043983.01.T01 AT2G01520 28.947 152 105 3 1 151 1 150 6.05e-16 70.9
MsG0880043983.01.T01 AT4G23680 29.677 155 100 4 1 151 1 150 1.99e-15 69.3
MsG0880043983.01.T01 AT1G35310 26.056 142 102 3 13 153 12 151 2.23e-14 66.6
MsG0880043983.01.T01 AT4G14060 29.677 155 100 4 1 151 1 150 2.45e-14 66.6
MsG0880043983.01.T01 AT1G14940 28.477 151 105 3 1 150 1 149 2.08e-13 64.3
MsG0880043983.01.T01 AT2G01530 27.632 152 107 3 1 151 1 150 2.99e-13 63.9
MsG0880043983.01.T01 AT1G30990 29.801 151 102 4 1 150 1 148 4.90e-13 63.2
MsG0880043983.01.T01 AT1G14950 28.125 160 106 4 1 156 1 155 4.94e-13 63.2
MsG0880043983.01.T01 AT3G26460 30.380 158 96 6 1 151 1 151 4.79e-11 58.2

Find 35 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGGTACTTACAAGCTCTT+TGG 0.306715 8:-33754707 MsG0880043983.01.T01:CDS
ATGGCACTGCTGCTGCTAAA+TGG 0.310932 8:-33754622 MsG0880043983.01.T01:CDS
AAGGTACTTACAAGCTCTTT+GGG 0.349654 8:-33754706 MsG0880043983.01.T01:CDS
CACCCTTATCAATTACTTCA+AGG 0.382574 8:+33754645 None:intergenic
AGATATTGATGAGCACTATA+AGG 0.414096 8:-33754681 MsG0880043983.01.T01:CDS
GGTAAACTTATTACTGAACT+TGG 0.415223 8:-33755471 MsG0880043983.01.T01:CDS
CATCCTTGAAGTAATTGATA+AGG 0.438738 8:-33754648 MsG0880043983.01.T01:CDS
ATCTTCACCCTCATGCAGCT+TGG 0.445602 8:+33755362 None:intergenic
AAGTAGTCCATCCAGCCATT+TGG 0.456227 8:+33754560 None:intergenic
AAACACTGGACTTATGTCAT+AGG 0.470664 8:-33755318 MsG0880043983.01.T01:intron
ATCCTTGAAGTAATTGATAA+GGG 0.486541 8:-33754647 MsG0880043983.01.T01:CDS
TGAAATAACAACAGATGGTA+AGG 0.489687 8:-33754777 MsG0880043983.01.T01:intron
AGCTGCATGAGGGTGAAGAT+TGG 0.493681 8:-33755359 MsG0880043983.01.T01:CDS
TATGACTTATAGAGCAACCT+TGG 0.515175 8:+33754493 None:intergenic
TGATGCTCATCTTGTCAAGC+AGG 0.523708 8:-33754516 MsG0880043983.01.T01:CDS
ATATTGACCCTCCAAATGGC+TGG 0.527845 8:-33754571 MsG0880043983.01.T01:CDS
GAGGATATTGACCCTCCAAA+TGG 0.539495 8:-33754575 MsG0880043983.01.T01:CDS
GTAAACTTATTACTGAACTT+GGG 0.542207 8:-33755470 MsG0880043983.01.T01:CDS
AGATTGGCATCACACTGATT+CGG 0.545548 8:-33755343 MsG0880043983.01.T01:CDS
ATTGATGAACAAAACAAGAA+AGG 0.549109 8:-33754725 MsG0880043983.01.T01:CDS
GAAGTAATTGATAAGGGTGA+TGG 0.549119 8:-33754641 MsG0880043983.01.T01:CDS
TAGTCCATCCAGCCATTTGG+AGG 0.551025 8:+33754563 None:intergenic
ACACTGATTCGGTCAAACAC+TGG 0.556043 8:-33755332 MsG0880043983.01.T01:CDS
TGACCCTCCAAATGGCTGGA+TGG 0.557517 8:-33754567 MsG0880043983.01.T01:CDS
GGATCAAAACACCCGCTGAA+AGG 0.578575 8:-33755449 MsG0880043983.01.T01:CDS
TCATCTTGTCAAGCAGGCCA+AGG 0.591246 8:-33754510 MsG0880043983.01.T01:CDS
TCATGAAACCAAGCTGCATG+AGG 0.602712 8:-33755370 MsG0880043983.01.T01:CDS
AGGTACTTACAAGCTCTTTG+GGG 0.621914 8:-33754705 MsG0880043983.01.T01:CDS
CATGAAACCAAGCTGCATGA+GGG 0.630684 8:-33755369 MsG0880043983.01.T01:CDS
AGTTTGAAGAACCTTTCAGC+GGG 0.631322 8:+33755438 None:intergenic
GGTACTTACAAGCTCTTTGG+GGG 0.640678 8:-33754704 MsG0880043983.01.T01:CDS
GGTACACACATGTCATGAGA+AGG 0.648648 8:-33754756 MsG0880043983.01.T01:CDS
AGTCCATCCAGCCATTTGGA+GGG 0.649199 8:+33754564 None:intergenic
TAGTTTGAAGAACCTTTCAG+CGG 0.662028 8:+33755437 None:intergenic
TGAATATGAGAAGATCAATG+AGG 0.699788 8:-33754594 MsG0880043983.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATAATATTTGTCTAATA+TGG - Chr8:33755019-33755038 MsG0880043983.01.T01:intron 10.0%
!! AATCTTACTAAAAAATGTTA+AGG + Chr8:33755142-33755161 None:intergenic 15.0%
!! TAGACAAATATTATTATTGA+TGG + Chr8:33755016-33755035 None:intergenic 15.0%
!!! TATTTTATTGAAAATAGCAA+CGG + Chr8:33754835-33754854 None:intergenic 15.0%
!! AATTAAAGAGATGTACAAAA+AGG - Chr8:33755111-33755130 MsG0880043983.01.T01:intron 20.0%
!! ACAAATATTATTATTGATGG+TGG + Chr8:33755013-33755032 None:intergenic 20.0%
!!! GATCTCTAAAAAATGTTTAA+GGG - Chr8:33755041-33755060 MsG0880043983.01.T01:intron 20.0%
!!! TAAACTGCTGTTATTTTTTT+TGG - Chr8:33755169-33755188 MsG0880043983.01.T01:intron 20.0%
! AGCAAAAAATTGTACAATAC+TGG + Chr8:33754977-33754996 None:intergenic 25.0%
! ATCCTTGAAGTAATTGATAA+GGG - Chr8:33755335-33755354 MsG0880043983.01.T01:CDS 25.0%
! ATTGATGAACAAAACAAGAA+AGG - Chr8:33755257-33755276 MsG0880043983.01.T01:intron 25.0%
! GATCCTTATTAAAAATGGAA+AGG + Chr8:33754955-33754974 None:intergenic 25.0%
! GTAAACTTATTACTGAACTT+GGG - Chr8:33754512-33754531 MsG0880043983.01.T01:CDS 25.0%
! TACTGGATCCTTATTAAAAA+TGG + Chr8:33754960-33754979 None:intergenic 25.0%
! TGTTGTACTACAAATGATAA+CGG + Chr8:33755071-33755090 None:intergenic 25.0%
!! GGATCTCTAAAAAATGTTTA+AGG - Chr8:33755040-33755059 MsG0880043983.01.T01:intron 25.0%
!!! GATCCTTTCCATTTTTAATA+AGG - Chr8:33754949-33754968 MsG0880043983.01.T01:intron 25.0%
AGATATTGATGAGCACTATA+AGG - Chr8:33755301-33755320 MsG0880043983.01.T01:intron 30.0%
CATCCTTGAAGTAATTGATA+AGG - Chr8:33755334-33755353 MsG0880043983.01.T01:CDS 30.0%
GGTAAACTTATTACTGAACT+TGG - Chr8:33754511-33754530 MsG0880043983.01.T01:CDS 30.0%
TGAAATAACAACAGATGGTA+AGG - Chr8:33755205-33755224 MsG0880043983.01.T01:intron 30.0%
TGAATATGAGAAGATCAATG+AGG - Chr8:33755388-33755407 MsG0880043983.01.T01:CDS 30.0%
!! ACCATCTGTTGTTATTTCAT+GGG + Chr8:33755204-33755223 None:intergenic 30.0%
!! TACCATCTGTTGTTATTTCA+TGG + Chr8:33755205-33755224 None:intergenic 30.0%
AAACACTGGACTTATGTCAT+AGG - Chr8:33754664-33754683 MsG0880043983.01.T01:CDS 35.0%
CAAAATCATCTTGTTGGACT+TGG - Chr8:33754719-33754738 MsG0880043983.01.T01:CDS 35.0%
CACCCTTATCAATTACTTCA+AGG + Chr8:33755340-33755359 None:intergenic 35.0%
GAAGTAATTGATAAGGGTGA+TGG - Chr8:33755341-33755360 MsG0880043983.01.T01:CDS 35.0%
TAGTTTGAAGAACCTTTCAG+CGG + Chr8:33754548-33754567 None:intergenic 35.0%
TGCCAACAAAATCATCTTGT+TGG - Chr8:33754713-33754732 MsG0880043983.01.T01:CDS 35.0%
! AAGGTACTTACAAGCTCTTT+GGG - Chr8:33755276-33755295 MsG0880043983.01.T01:intron 35.0%
! GTCCAACAAGATGATTTTGT+TGG + Chr8:33754718-33754737 None:intergenic 35.0%
!! AAAGGTACTTACAAGCTCTT+TGG - Chr8:33755275-33755294 MsG0880043983.01.T01:intron 35.0%
AGGTACTTACAAGCTCTTTG+GGG - Chr8:33755277-33755296 MsG0880043983.01.T01:intron 40.0%
AGTTTGAAGAACCTTTCAGC+GGG + Chr8:33754547-33754566 None:intergenic 40.0%
GCCCATGAAATAACAACAGA+TGG - Chr8:33755200-33755219 MsG0880043983.01.T01:intron 40.0%
! AGATTGGCATCACACTGATT+CGG - Chr8:33754639-33754658 MsG0880043983.01.T01:CDS 40.0%
AAGTAGTCCATCCAGCCATT+TGG + Chr8:33755425-33755444 None:intergenic 45.0%
ATATTGACCCTCCAAATGGC+TGG - Chr8:33755411-33755430 MsG0880043983.01.T01:CDS 45.0%
CATGAAACCAAGCTGCATGA+GGG - Chr8:33754613-33754632 MsG0880043983.01.T01:CDS 45.0%
GAGGATATTGACCCTCCAAA+TGG - Chr8:33755407-33755426 MsG0880043983.01.T01:CDS 45.0%
GGTACACACATGTCATGAGA+AGG - Chr8:33755226-33755245 MsG0880043983.01.T01:intron 45.0%
GGTACTTACAAGCTCTTTGG+GGG - Chr8:33755278-33755297 MsG0880043983.01.T01:intron 45.0%
TCATGAAACCAAGCTGCATG+AGG - Chr8:33754612-33754631 MsG0880043983.01.T01:CDS 45.0%
TGATGCTCATCTTGTCAAGC+AGG - Chr8:33755466-33755485 MsG0880043983.01.T01:CDS 45.0%
!! ACACTGATTCGGTCAAACAC+TGG - Chr8:33754650-33754669 MsG0880043983.01.T01:CDS 45.0%
AGCTGCATGAGGGTGAAGAT+TGG - Chr8:33754623-33754642 MsG0880043983.01.T01:CDS 50.0%
AGTCCATCCAGCCATTTGGA+GGG + Chr8:33755421-33755440 None:intergenic 50.0%
ATCTTCACCCTCATGCAGCT+TGG + Chr8:33754623-33754642 None:intergenic 50.0%
ATGGCACTGCTGCTGCTAAA+TGG - Chr8:33755360-33755379 MsG0880043983.01.T01:CDS 50.0%
GGATCAAAACACCCGCTGAA+AGG - Chr8:33754533-33754552 MsG0880043983.01.T01:CDS 50.0%
TAGTCCATCCAGCCATTTGG+AGG + Chr8:33755422-33755441 None:intergenic 50.0%
TCATCTTGTCAAGCAGGCCA+AGG - Chr8:33755472-33755491 MsG0880043983.01.T01:CDS 50.0%
TGACCCTCCAAATGGCTGGA+TGG - Chr8:33755415-33755434 MsG0880043983.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr8 gene 33754499 33755505 33754499 ID=MsG0880043983.01;Name=MsG0880043983.01
Chr8 mRNA 33754499 33755505 33754499 ID=MsG0880043983.01.T01;Parent=MsG0880043983.01;Name=MsG0880043983.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0|1|1|1|2|0|157
Chr8 exon 33755319 33755505 33755319 ID=MsG0880043983.01.T01:exon:10246;Parent=MsG0880043983.01.T01
Chr8 exon 33754499 33754785 33754499 ID=MsG0880043983.01.T01:exon:10245;Parent=MsG0880043983.01.T01
Chr8 CDS 33755319 33755505 33755319 ID=MsG0880043983.01.T01:cds;Parent=MsG0880043983.01.T01
Chr8 CDS 33754499 33754785 33754499 ID=MsG0880043983.01.T01:cds;Parent=MsG0880043983.01.T01
Gene Sequence

>MsG0880043983.01.T01

ATGGTGCTTGCTGGTAAACTTATTACTGAACTTGGGATCAAAACACCCGCTGAAAGGTTCTTCAAACTATTTGCATCAAAACTTCATGAAGTGCAAAAACACTGTGAAAGAGTTCATGAAACCAAGCTGCATGAGGGTGAAGATTGGCATCACACTGATTCGGTCAAACACTGGACTTATGTCATAGATGGTAAGGTACACACATGTCATGAGAAGGTTGAAGAAATTGATGAACAAAACAAGAAAGGTACTTACAAGCTCTTTGGGGGAGATATTGATGAGCACTATAAGGACTTTAAGCTCATCCTTGAAGTAATTGATAAGGGTGATGGCACTGCTGCTGCTAAATGGACAGTTGAATATGAGAAGATCAATGAGGATATTGACCCTCCAAATGGCTGGATGGACTACTTATGCAAATGTACTCGAGATATTGATGCTCATCTTGTCAAGCAGGCCAAGGTTGCTCTATAA

Protein sequence

>MsG0880043983.01.T01

MVLAGKLITELGIKTPAERFFKLFASKLHEVQKHCERVHETKLHEGEDWHHTDSVKHWTYVIDGKVHTCHEKVEEIDEQNKKGTYKLFGGDIDEHYKDFKLILEVIDKGDGTAAAKWTVEYEKINEDIDPPNGWMDYLCKCTRDIDAHLVKQAKVAL*