AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010596.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010596.01.T01 MTR_2g088230 95.652 92 4 0 31 122 17 108 6.49e-62 184
MsG0280010596.01.T01 MTR_4g048510 95.506 89 4 0 34 122 32 120 3.39e-60 181
MsG0280010596.01.T01 MTR_4g101090 83.146 89 15 0 34 122 32 120 2.76e-51 158
MsG0280010596.01.T01 MTR_2g023430 83.721 86 14 0 34 119 32 117 4.20e-51 158
MsG0280010596.01.T01 MTR_4g123760 58.140 86 36 0 34 119 33 118 1.15e-37 124
MsG0280010596.01.T01 MTR_1g086310 56.977 86 37 0 34 119 33 118 3.87e-33 112
MsG0280010596.01.T01 MTR_4g037225 64.286 84 28 1 34 115 32 115 7.48e-33 112
MsG0280010596.01.T01 MTR_7g096540 58.065 62 26 0 58 119 1 62 1.33e-25 91.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010596.01.T01 AT2G05630 92.135 89 7 0 34 122 32 120 6.49e-58 175
MsG0280010596.01.T01 AT2G05630 92.135 89 7 0 34 122 76 164 1.47e-57 176
MsG0280010596.01.T01 AT1G62040 90.909 88 8 0 34 121 32 119 3.37e-56 171
MsG0280010596.01.T01 AT4G21980 88.636 88 10 0 34 121 32 119 8.04e-54 165
MsG0280010596.01.T01 AT4G21980 88.636 88 10 0 34 121 47 134 1.07e-53 165
MsG0280010596.01.T01 AT4G04620 88.372 86 10 0 34 119 32 117 6.17e-53 162
MsG0280010596.01.T01 AT4G04620 88.372 86 10 0 34 119 32 117 6.17e-53 162
MsG0280010596.01.T01 AT4G04620 88.372 86 10 0 34 119 32 117 6.17e-53 162
MsG0280010596.01.T01 AT3G60640 82.022 89 16 0 34 122 32 120 1.30e-51 159
MsG0280010596.01.T01 AT4G16520 73.000 100 27 0 20 119 18 117 1.36e-50 156
MsG0280010596.01.T01 AT4G16520 81.609 87 16 0 34 120 32 118 6.86e-50 155
MsG0280010596.01.T01 AT4G16520 81.609 87 16 0 34 120 32 118 6.86e-50 155
MsG0280010596.01.T01 AT2G45170 81.395 86 16 0 34 119 33 118 2.29e-49 154
MsG0280010596.01.T01 AT2G45170 81.395 86 16 0 34 119 33 118 2.29e-49 154
MsG0280010596.01.T01 AT3G15580 58.140 86 36 0 34 119 30 115 1.08e-34 116
MsG0280010596.01.T01 AT3G06420 54.651 86 39 0 34 119 34 119 7.36e-33 112
MsG0280010596.01.T01 AT1G62040 82.540 63 11 0 34 96 32 94 1.56e-32 110

Find 25 sgRNAs with CRISPR-Local

Find 266 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTTGCTGTGACAATTTAT+TGG 0.241677 2:-71992982 MsG0280010596.01.T01:three_prime_UTR
TACAGCGGGGAGAACACTTT+TGG 0.291853 2:-71993081 MsG0280010596.01.T01:CDS
CTTTGCTGTGACAATTTATT+GGG 0.378455 2:-71992981 MsG0280010596.01.T01:three_prime_UTR
CAATTTGAATACAAACATAA+AGG 0.390713 2:+71992901 None:intergenic
GGGTGTAGGTTATTGTTGAA+AGG 0.421063 2:-71994402 MsG0280010596.01.T01:intron
CCCCGCTGTAAGTCATATAC+AGG 0.458740 2:+71993094 None:intergenic
AAGTAGTTGCAGATTAGATA+AGG 0.465664 2:+71992932 None:intergenic
TCCGCACCACATACACGAAC+TGG 0.481973 2:+71993345 None:intergenic
GGTGTAGGTTATTGTTGAAA+GGG 0.488338 2:-71994401 MsG0280010596.01.T01:intron
AAAATGAAGATGGCCTTCTC+GGG 0.489019 2:+71993305 None:intergenic
GAGGAAAACAAGGATGAAGA+TGG 0.507080 2:-71993120 MsG0280010596.01.T01:CDS
TATGATGTCTGCAATATATG+AGG 0.514512 2:-71993139 MsG0280010596.01.T01:CDS
TCCTGTATATGACTTACAGC+GGG 0.516758 2:-71993095 MsG0280010596.01.T01:CDS
TGGCCTACAGTTAGATCAGC+AGG 0.520566 2:+71993365 None:intergenic
GTTCCTGCTGATCTAACTGT+AGG 0.544817 2:-71993368 MsG0280010596.01.T01:CDS
TGGTGAAATGGCTTCTGATG+AGG 0.574708 2:-71997304 None:intergenic
TCATATATATTACCGGTTGG+TGG 0.577762 2:+71993266 None:intergenic
TGTAGGCCAGTTCGTGTATG+TGG 0.582982 2:-71993351 MsG0280010596.01.T01:CDS
GCCAGTTCGTGTATGTGGTG+CGG 0.607938 2:-71993346 MsG0280010596.01.T01:CDS
AATCAAATTGAGTCCCGAGA+AGG 0.616573 2:-71993318 MsG0280010596.01.T01:CDS
AGATTAGATAAGGATTGTCA+TGG 0.617712 2:+71992942 None:intergenic
TGCAATATATGAGGAAAACA+AGG 0.622289 2:-71993130 MsG0280010596.01.T01:CDS
AAAGCATAACTATTTCACAT+AGG 0.623046 2:+71993000 None:intergenic
TTCCTGTATATGACTTACAG+CGG 0.658464 2:-71993096 MsG0280010596.01.T01:CDS
CCTGTATATGACTTACAGCG+GGG 0.753998 2:-71993094 MsG0280010596.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCTAAAAATAAAAAACA+GGG - Chr2:71994048-71994067 MsG0280010596.01.T01:intron 10.0%
!! ATAATAATCATATATATTAC+CGG + Chr2:71996952-71996971 None:intergenic 10.0%
!! TAAATCTAAAAATAAAAAAC+AGG - Chr2:71994047-71994066 MsG0280010596.01.T01:intron 10.0%
!!! AAAAAACAATTTTTTTTTGT+TGG + Chr2:71994411-71994430 None:intergenic 10.0%
!! AATATGAGTATATTATATGT+AGG + Chr2:71994381-71994400 None:intergenic 15.0%
!! AGGTATTAAAAATTGTAAAT+TGG + Chr2:71993240-71993259 None:intergenic 15.0%
!! ATATGAGTATATTATATGTA+GGG + Chr2:71994380-71994399 None:intergenic 15.0%
!! ATCACTTATAAAATTTGAAA+AGG - Chr2:71993676-71993695 MsG0280010596.01.T01:intron 15.0%
!! ATGATGTAACTTTATAATTA+TGG - Chr2:71993184-71993203 MsG0280010596.01.T01:intron 15.0%
!! GATTTGTAATTTGAAAAATT+GGG - Chr2:71994964-71994983 MsG0280010596.01.T01:intron 15.0%
!! TAACCTAAAAAAACAAAAAT+TGG + Chr2:71994845-71994864 None:intergenic 15.0%
!!! AAATTAGAATTTTTCAACAA+AGG - Chr2:71993649-71993668 MsG0280010596.01.T01:intron 15.0%
!!! AATTTATCGATTTTATGTTT+TGG - Chr2:71994878-71994897 MsG0280010596.01.T01:intron 15.0%
!!! CATATAATATACTCATATTT+TGG - Chr2:71994382-71994401 MsG0280010596.01.T01:CDS 15.0%
!!! GATTAAGGAATATTTTATTT+TGG - Chr2:71993929-71993948 MsG0280010596.01.T01:intron 15.0%
!!! TAAATACAATAGTGATATTT+TGG - Chr2:71994461-71994480 MsG0280010596.01.T01:intron 15.0%
!!! TAATGAAATTTTGCAAAATT+AGG - Chr2:71995359-71995378 MsG0280010596.01.T01:intron 15.0%
!!! TGATTTGTAATTTGAAAAAT+TGG - Chr2:71994963-71994982 MsG0280010596.01.T01:intron 15.0%
!!! TTTGTTTTTTTAGGTTATAT+TGG - Chr2:71994848-71994867 MsG0280010596.01.T01:intron 15.0%
!!! TTTTTGGTATTTTTTACTAA+AGG + Chr2:71993555-71993574 None:intergenic 15.0%
!! AGTTAATTGGTGAATAAAAA+AGG - Chr2:71996301-71996320 MsG0280010596.01.T01:intron 20.0%
!! CAAAAAAGTTATTTAGAGAT+CGG - Chr2:71993569-71993588 MsG0280010596.01.T01:intron 20.0%
!! CATTAGTATTATTTCCTTTA+TGG - Chr2:71996987-71997006 MsG0280010596.01.T01:intron 20.0%
!! GTTAATTGGTGAATAAAAAA+GGG - Chr2:71996302-71996321 MsG0280010596.01.T01:intron 20.0%
!! TATGAGTATATTATATGTAG+GGG + Chr2:71994379-71994398 None:intergenic 20.0%
!! TGTAGATCAAATATATTAGT+GGG + Chr2:71996390-71996409 None:intergenic 20.0%
!! TTATAAAGTTACATCATCAT+TGG + Chr2:71993181-71993200 None:intergenic 20.0%
!! TTGTAGATCAAATATATTAG+TGG + Chr2:71996391-71996410 None:intergenic 20.0%
!!! AAGACATAGTTTCTTTTTTT+GGG + Chr2:71994151-71994170 None:intergenic 20.0%
!!! AATGTGAAATATTTTCACAA+GGG - Chr2:71997156-71997175 MsG0280010596.01.T01:intron 20.0%
!!! ATTAATCTGGTTAATCTTTT+GGG - Chr2:71995786-71995805 MsG0280010596.01.T01:intron 20.0%
!!! GAAATATTTACACTTTTACT+TGG - Chr2:71996003-71996022 MsG0280010596.01.T01:intron 20.0%
!!! GATTTTATGTTTTGGTTATT+AGG - Chr2:71994886-71994905 MsG0280010596.01.T01:intron 20.0%
!!! TAATGTGAAATATTTTCACA+AGG - Chr2:71997155-71997174 MsG0280010596.01.T01:intron 20.0%
!!! TATCTGTTCTTTTATTAATC+TGG - Chr2:71995773-71995792 MsG0280010596.01.T01:intron 20.0%
!!! TATTAATCTGGTTAATCTTT+TGG - Chr2:71995785-71995804 MsG0280010596.01.T01:intron 20.0%
!!! TGATTTTCAATATATGTTTG+TGG - Chr2:71996097-71996116 MsG0280010596.01.T01:intron 20.0%
!!! TTATTTTGGACTTTAATTCT+GGG - Chr2:71993943-71993962 MsG0280010596.01.T01:intron 20.0%
!!! TTCCTTTAAAGATGAAAATT+TGG - Chr2:71995019-71995038 MsG0280010596.01.T01:intron 20.0%
!!! TTGCCAATTTTTGTTTTTTT+AGG - Chr2:71994839-71994858 MsG0280010596.01.T01:intron 20.0%
!!! TTTATTTTGGACTTTAATTC+TGG - Chr2:71993942-71993961 MsG0280010596.01.T01:intron 20.0%
!!! TTTGGTGATAAATAATGTTA+CGG - Chr2:71994479-71994498 MsG0280010596.01.T01:intron 20.0%
!!! TTTTAGATTTTCGATTTTGT+CGG - Chr2:71994333-71994352 MsG0280010596.01.T01:intron 20.0%
!!! TTTTGAAATGATTGTTTACA+TGG - Chr2:71993750-71993769 MsG0280010596.01.T01:intron 20.0%
! AAACTCAAAAACCAAAACAA+CGG - Chr2:71993705-71993724 MsG0280010596.01.T01:intron 25.0%
! AAAGCATAACTATTTCACAT+AGG + Chr2:71997211-71997230 None:intergenic 25.0%
! AAGTTGTAAGCAATGAATTA+AGG - Chr2:71995419-71995438 MsG0280010596.01.T01:intron 25.0%
! AATTAGAGAATGAAAGAGAT+TGG + Chr2:71994686-71994705 None:intergenic 25.0%
! AATTCACATAACGATCATAA+TGG + Chr2:71994996-71995015 None:intergenic 25.0%
! AGGATAAAAAAGACTCATTT+AGG - Chr2:71995262-71995281 MsG0280010596.01.T01:intron 25.0%
! ATAAACTTTATGATTCAGAG+AGG - Chr2:71995646-71995665 MsG0280010596.01.T01:intron 25.0%
! GTTAGCAGGAAAAAAAATTA+GGG + Chr2:71993488-71993507 None:intergenic 25.0%
! GTTATCATCAAAACTCTTTA+AGG - Chr2:71993261-71993280 MsG0280010596.01.T01:intron 25.0%
! TAATATTTGTTCAAGTGTAC+AGG + Chr2:71993211-71993230 None:intergenic 25.0%
! TAATCATATATATTACCGGT+TGG + Chr2:71996948-71996967 None:intergenic 25.0%
! TATAGTCCTTGATTGTATAT+GGG - Chr2:71995155-71995174 MsG0280010596.01.T01:intron 25.0%
! TGAAGTTCAAACATAACAAA+GGG + Chr2:71996520-71996539 None:intergenic 25.0%
! TGTTAGCAGGAAAAAAAATT+AGG + Chr2:71993489-71993508 None:intergenic 25.0%
! TTAATAAATGAACAACGTAC+TGG + Chr2:71995720-71995739 None:intergenic 25.0%
! TTAGCAGGAAAAAAAATTAG+GGG + Chr2:71993487-71993506 None:intergenic 25.0%
! TTATAGTCCTTGATTGTATA+TGG - Chr2:71995154-71995173 MsG0280010596.01.T01:intron 25.0%
! TTGAAGTTCAAACATAACAA+AGG + Chr2:71996521-71996540 None:intergenic 25.0%
!! AAAAGTAGCAATTTTTGCAT+TGG + Chr2:71994219-71994238 None:intergenic 25.0%
!! AACAACAAAAATTTGAAGCT+TGG + Chr2:71993129-71993148 None:intergenic 25.0%
!! AAGAATTAGGAACGTTATTT+TGG - Chr2:71995102-71995121 MsG0280010596.01.T01:intron 25.0%
!! AATTGGTAACTTAGTAGTAA+TGG + Chr2:71995606-71995625 None:intergenic 25.0%
!! AGCCAAATTTTCATCTTTAA+AGG + Chr2:71995024-71995043 None:intergenic 25.0%
!! ATGTGAAATATTTTCACAAG+GGG - Chr2:71997157-71997176 MsG0280010596.01.T01:intron 25.0%
!! ATTTTAAACCAAATCAGGTA+AGG - Chr2:71994285-71994304 MsG0280010596.01.T01:intron 25.0%
!! CAAAATTTGATTTACATCTG+TGG - Chr2:71993801-71993820 MsG0280010596.01.T01:intron 25.0%
!! CCATAATTTTAAACCAAATC+AGG - Chr2:71994280-71994299 MsG0280010596.01.T01:intron 25.0%
!! CTACAACAAAAGAGTTATTT+TGG - Chr2:71996405-71996424 MsG0280010596.01.T01:intron 25.0%
!! GAAAAATTCTAATTTTCCGT+GGG + Chr2:71993644-71993663 None:intergenic 25.0%
!! TGAAAAATTCTAATTTTCCG+TGG + Chr2:71993645-71993664 None:intergenic 25.0%
!!! AAAACCACTTTTCCTTTAAT+TGG + Chr2:71996678-71996697 None:intergenic 25.0%
!!! AAACCACTTTTCCTTTAATT+GGG + Chr2:71996677-71996696 None:intergenic 25.0%
!!! AAAGAGTTTTGATGATAACA+AGG + Chr2:71993260-71993279 None:intergenic 25.0%
!!! AACTTTTTCTTGTCAATATC+TGG + Chr2:71995850-71995869 None:intergenic 25.0%
!!! ATTGTTGTGCTGATATTTTT+TGG - Chr2:71995387-71995406 MsG0280010596.01.T01:intron 25.0%
!!! CAAAAGAGTTATTTTGGTTT+AGG - Chr2:71996411-71996430 MsG0280010596.01.T01:intron 25.0%
!!! CAAGACATAGTTTCTTTTTT+TGG + Chr2:71994152-71994171 None:intergenic 25.0%
!!! CCTGATTTGGTTTAAAATTA+TGG + Chr2:71994283-71994302 None:intergenic 25.0%
!!! CGATCTCTAAATAACTTTTT+TGG + Chr2:71993571-71993590 None:intergenic 25.0%
!!! TGTAAAATGACTTTTGAAAC+TGG - Chr2:71996697-71996716 MsG0280010596.01.T01:intron 25.0%
!!! TTGTAATTTTGATACGTTCA+AGG - Chr2:71995291-71995310 MsG0280010596.01.T01:intron 25.0%
AAAAGCCACTTACAGTTAAT+TGG - Chr2:71996288-71996307 MsG0280010596.01.T01:intron 30.0%
AAACTAAATAACGTGTCGAA+CGG - Chr2:71995214-71995233 MsG0280010596.01.T01:intron 30.0%
AAAGCAGAGATTAGAATAGA+AGG + Chr2:71996249-71996268 None:intergenic 30.0%
AACGTATAAGGCTAAACTTT+AGG + Chr2:71994539-71994558 None:intergenic 30.0%
AAGTAGTTGCAGATTAGATA+AGG + Chr2:71997279-71997298 None:intergenic 30.0%
AATCCTGTGTGTCAAAAATA+TGG - Chr2:71996473-71996492 MsG0280010596.01.T01:intron 30.0%
AATCTCTCTTTGATTGATTG+CGG + Chr2:71994594-71994613 None:intergenic 30.0%
AATGAGCAAATAAAGAGCTA+TGG + Chr2:71993153-71993172 None:intergenic 30.0%
AGATTAGATAAGGATTGTCA+TGG + Chr2:71997269-71997288 None:intergenic 30.0%
AGGAAAAAAAATTAGGGGAA+AGG + Chr2:71993482-71993501 None:intergenic 30.0%
AGTTAAAATAAGACTTCCCA+CGG - Chr2:71993625-71993644 MsG0280010596.01.T01:intron 30.0%
ATTATGCTGCAACAGAATTA+GGG + Chr2:71994750-71994769 None:intergenic 30.0%
ATTCACCAATTAACTGTAAG+TGG + Chr2:71996296-71996315 None:intergenic 30.0%
CAAATTCAAAAACCTCAACA+AGG + Chr2:71995960-71995979 None:intergenic 30.0%
CAGAAAGTAATTACTGAAAC+AGG + Chr2:71995917-71995936 None:intergenic 30.0%
CCAAACAAAATAAGATACCA+AGG + Chr2:71996154-71996173 None:intergenic 30.0%
CTAAAAAAAAAAAAGCCACG+TGG + Chr2:71993402-71993421 None:intergenic 30.0%
GAAAATGACCTTTATGTTTG+TGG - Chr2:71997299-71997318 MsG0280010596.01.T01:CDS 30.0%
GGAACAACAAAAGAAGAATT+AGG - Chr2:71995089-71995108 MsG0280010596.01.T01:intron 30.0%
GGGATAATATTAGTAGAATG+AGG - Chr2:71993989-71994008 MsG0280010596.01.T01:intron 30.0%
GTTCTGTAATGTGTCTTATA+GGG - Chr2:71996061-71996080 MsG0280010596.01.T01:intron 30.0%
GTTCTTCACAAAAATGAAGA+TGG + Chr2:71996916-71996935 None:intergenic 30.0%
TATGATGTCTGCAATATATG+AGG - Chr2:71997069-71997088 MsG0280010596.01.T01:intron 30.0%
TGATGAGATTAGATGATGAA+GGG - Chr2:71993894-71993913 MsG0280010596.01.T01:intron 30.0%
TGATGTCTGAATTAGAACAT+TGG - Chr2:71995541-71995560 MsG0280010596.01.T01:intron 30.0%
TGCAATATATGAGGAAAACA+AGG - Chr2:71997078-71997097 MsG0280010596.01.T01:intron 30.0%
TGTTCTGTAATGTGTCTTAT+AGG - Chr2:71996060-71996079 MsG0280010596.01.T01:intron 30.0%
TTCCTTAAACAATGCTCTTA+GGG + Chr2:71994128-71994147 None:intergenic 30.0%
TTCCTTTATGGTGATTAACT+TGG - Chr2:71996999-71997018 MsG0280010596.01.T01:intron 30.0%
! AAAGAGATTGGTTACCAAAA+GGG + Chr2:71994674-71994693 None:intergenic 30.0%
! ACAAATCAGAGAGCATAAAT+TGG + Chr2:71995623-71995642 None:intergenic 30.0%
! ATCGGATTTTTCATGATTTC+TGG - Chr2:71993587-71993606 MsG0280010596.01.T01:intron 30.0%
! ATTTTAAATCCTCCAATGTG+AGG + Chr2:71995332-71995351 None:intergenic 30.0%
! CTTTGCTGTGACAATTTATT+GGG - Chr2:71997227-71997246 MsG0280010596.01.T01:CDS 30.0%
! GGATTTGAAATTCATGTGAT+CGG - Chr2:71994907-71994926 MsG0280010596.01.T01:intron 30.0%
! TAATGAAAGTATGACCACTA+TGG + Chr2:71996036-71996055 None:intergenic 30.0%
! TCTCTGCTTTATTGTTCTAA+TGG - Chr2:71996259-71996278 MsG0280010596.01.T01:intron 30.0%
! TGAGGTTTTTGAATTTGCAT+GGG - Chr2:71995963-71995982 MsG0280010596.01.T01:intron 30.0%
! TTGAGGTTTTTGAATTTGCA+TGG - Chr2:71995962-71995981 MsG0280010596.01.T01:intron 30.0%
! TTTTGAGCAAAAAAGTCTGT+TGG + Chr2:71993310-71993329 None:intergenic 30.0%
!! CTTTCTGCATTAATTTGTTG+TGG - Chr2:71995930-71995949 MsG0280010596.01.T01:intron 30.0%
!! GGTAAGGTCTTATACATTTT+CGG - Chr2:71994301-71994320 MsG0280010596.01.T01:intron 30.0%
!! GTAAGGTCTTATACATTTTC+GGG - Chr2:71994302-71994321 MsG0280010596.01.T01:intron 30.0%
!!! AACATTGGTTTTCTCTTTGT+GGG - Chr2:71995556-71995575 MsG0280010596.01.T01:intron 30.0%
!!! CCTTGGTATCTTATTTTGTT+TGG - Chr2:71996151-71996170 MsG0280010596.01.T01:intron 30.0%
!!! CTGTTGTCACATGAATTTTT+TGG - Chr2:71993434-71993453 MsG0280010596.01.T01:intron 30.0%
!!! CTTTTGTTGTTCCTTTACTT+GGG + Chr2:71995082-71995101 None:intergenic 30.0%
!!! TATCGTTTTTTGTTGATTGC+AGG - Chr2:71996811-71996830 MsG0280010596.01.T01:intron 30.0%
!!! TCTTATTTTGTTTGGTTGAG+TGG - Chr2:71996159-71996178 MsG0280010596.01.T01:intron 30.0%
!!! TCTTTTGTTGTTCCTTTACT+TGG + Chr2:71995083-71995102 None:intergenic 30.0%
!!! TTTTTTTTTAGAGAACCACG+TGG - Chr2:71993411-71993430 MsG0280010596.01.T01:intron 30.0%
AAACAAGATTGGGAAGGATT+TGG + Chr2:71994571-71994590 None:intergenic 35.0%
AAGAGAAAAGAACGAACCAA+GGG + Chr2:71994814-71994833 None:intergenic 35.0%
AATCACAAGTGTGTGTAGAA+GGG - Chr2:71993969-71993988 MsG0280010596.01.T01:intron 35.0%
AATGATGCGTTAGATGTGAT+TGG - Chr2:71993829-71993848 MsG0280010596.01.T01:intron 35.0%
ACAACGTACTGGAATTCTAT+CGG + Chr2:71995709-71995728 None:intergenic 35.0%
ATAAGATACCAAGGTTTGCA+AGG + Chr2:71996145-71996164 None:intergenic 35.0%
ATGAAAATATACCACCTGCA+TGG + Chr2:71996776-71996795 None:intergenic 35.0%
ATGCTTAAACAATGCCCTTA+GGG - Chr2:71994241-71994260 MsG0280010596.01.T01:intron 35.0%
ATTATATGTAGGGGTCTAAG+TGG + Chr2:71994370-71994389 None:intergenic 35.0%
ATTGATTGCGGAAACAAGAT+TGG + Chr2:71994582-71994601 None:intergenic 35.0%
CACCAAGTTAATCACCATAA+AGG + Chr2:71997004-71997023 None:intergenic 35.0%
CAGCATAATCATCATCATCA+TGG - Chr2:71994761-71994780 MsG0280010596.01.T01:intron 35.0%
CATTACATATGTACACGCAA+AGG + Chr2:71995749-71995768 None:intergenic 35.0%
GATTATGCTGCAACAGAATT+AGG + Chr2:71994751-71994770 None:intergenic 35.0%
GGGAGAAAGTGAATAGATTA+AGG - Chr2:71993914-71993933 MsG0280010596.01.T01:intron 35.0%
GGTGTAGGTTATTGTTGAAA+GGG - Chr2:71995807-71995826 MsG0280010596.01.T01:intron 35.0%
GTATAAGACCTTACCTGATT+TGG + Chr2:71994296-71994315 None:intergenic 35.0%
GTGATGAGATTAGATGATGA+AGG - Chr2:71993893-71993912 MsG0280010596.01.T01:intron 35.0%
GTTACTCGATTAATGTTAGC+AGG + Chr2:71993502-71993521 None:intergenic 35.0%
GTTCCTTAAACAATGCTCTT+AGG + Chr2:71994129-71994148 None:intergenic 35.0%
GTTTGTGGTATTAGACTTGA+TGG - Chr2:71996112-71996131 MsG0280010596.01.T01:intron 35.0%
TAATCACAAGTGTGTGTAGA+AGG - Chr2:71993968-71993987 MsG0280010596.01.T01:intron 35.0%
TAGAAGAGAAGAGAAGCAAA+GGG + Chr2:71994621-71994640 None:intergenic 35.0%
TATTAGTAGAATGAGGAGTG+AGG - Chr2:71993996-71994015 MsG0280010596.01.T01:intron 35.0%
TCGTAACCCATATACAATCA+AGG + Chr2:71995164-71995183 None:intergenic 35.0%
TTCCTGTATATGACTTACAG+CGG - Chr2:71997112-71997131 MsG0280010596.01.T01:intron 35.0%
TTGAAAGAAATCTTACCACG+TGG - Chr2:71993384-71993403 MsG0280010596.01.T01:intron 35.0%
TTGATTGCGGAAACAAGATT+GGG + Chr2:71994581-71994600 None:intergenic 35.0%
TTGTCCTCCAACAATACTTT+GGG - Chr2:71996559-71996578 MsG0280010596.01.T01:intron 35.0%
TTTGCTTCTCTTCTCTTCTA+GGG - Chr2:71994621-71994640 MsG0280010596.01.T01:intron 35.0%
TTTGTCCTCCAACAATACTT+TGG - Chr2:71996558-71996577 MsG0280010596.01.T01:intron 35.0%
! AAGAACATTTTACCACCAAC+CGG - Chr2:71996930-71996949 MsG0280010596.01.T01:intron 35.0%
! ATGGGTTGTTTATCGAAGTT+TGG - Chr2:71995981-71996000 MsG0280010596.01.T01:intron 35.0%
! ATTTTTGTCTGCCCAAGTAA+AGG - Chr2:71995068-71995087 MsG0280010596.01.T01:intron 35.0%
! CACTTTTCCTTTAATTGGGA+GGG + Chr2:71996673-71996692 None:intergenic 35.0%
! GAAAGAGATTGGTTACCAAA+AGG + Chr2:71994675-71994694 None:intergenic 35.0%
! GCTTTGCTGTGACAATTTAT+TGG - Chr2:71997226-71997245 MsG0280010596.01.T01:CDS 35.0%
! TCATATATATTACCGGTTGG+TGG + Chr2:71996945-71996964 None:intergenic 35.0%
! TGTCGAAATTTCCGTTGTTT+TGG + Chr2:71993719-71993738 None:intergenic 35.0%
!! AGGGGGCATACTATAATTTT+AGG - Chr2:71994086-71994105 MsG0280010596.01.T01:intron 35.0%
!! CAACCATATTTTTGACACAC+AGG + Chr2:71996479-71996498 None:intergenic 35.0%
!! CAACTAACCCAAAGTATTGT+TGG + Chr2:71996569-71996588 None:intergenic 35.0%
!! GGGGGCATACTATAATTTTA+GGG - Chr2:71994087-71994106 MsG0280010596.01.T01:intron 35.0%
!! TTTTGTTGATTGCAGGTATC+TGG - Chr2:71996818-71996837 MsG0280010596.01.T01:intron 35.0%
!!! ACATTGGTTTTCTCTTTGTG+GGG - Chr2:71995557-71995576 MsG0280010596.01.T01:intron 35.0%
!!! GAACATTGGTTTTCTCTTTG+TGG - Chr2:71995555-71995574 MsG0280010596.01.T01:intron 35.0%
!!! GCTTTTTCACTTCTGTTAGT+TGG - Chr2:71995477-71995496 MsG0280010596.01.T01:intron 35.0%
AAAAGTAGCAAAAGTGGCTG+TGG - Chr2:71996722-71996741 MsG0280010596.01.T01:intron 40.0%
AAATGCTAACAAGTGCCCTA+AGG + Chr2:71994259-71994278 None:intergenic 40.0%
AATGCTAACAAGTGCCCTAA+GGG + Chr2:71994258-71994277 None:intergenic 40.0%
AGAGAAAAGAACGAACCAAG+GGG + Chr2:71994813-71994832 None:intergenic 40.0%
AGGGAATCGAACCAAAGTAA+GGG - Chr2:71994067-71994086 MsG0280010596.01.T01:intron 40.0%
ATACCAAGGTTTGCAAGGAT+AGG + Chr2:71996140-71996159 None:intergenic 40.0%
ATCATAGCAGCTGTATGAAG+AGG + Chr2:71997055-71997074 None:intergenic 40.0%
ATCTCTTGTGTGATTGGTAG+GGG + Chr2:71993357-71993376 None:intergenic 40.0%
ATGAGGAGTGAGGATATCAT+CGG - Chr2:71994006-71994025 MsG0280010596.01.T01:intron 40.0%
ATTGGGAAGGATTTGGCAAT+TGG + Chr2:71994564-71994583 None:intergenic 40.0%
CAACGTACTGGAATTCTATC+GGG + Chr2:71995708-71995727 None:intergenic 40.0%
CAAGAGAAAAGAACGAACCA+AGG + Chr2:71994815-71994834 None:intergenic 40.0%
CATCGTAGGATAAAAGTCGT+AGG - Chr2:71995242-71995261 MsG0280010596.01.T01:intron 40.0%
CATCTCTTGTGTGATTGGTA+GGG + Chr2:71993358-71993377 None:intergenic 40.0%
CCTCCCAATTAAAGGAAAAG+TGG - Chr2:71996671-71996690 MsG0280010596.01.T01:intron 40.0%
CTAGAAGAGAAGAGAAGCAA+AGG + Chr2:71994622-71994641 None:intergenic 40.0%
CTGGAGAAAAGTAGCAAAAG+TGG - Chr2:71996716-71996735 MsG0280010596.01.T01:intron 40.0%
CTTTGCTTCTCTTCTCTTCT+AGG - Chr2:71994620-71994639 MsG0280010596.01.T01:intron 40.0%
GAGGAAAACAAGGATGAAGA+TGG - Chr2:71997088-71997107 MsG0280010596.01.T01:intron 40.0%
GATGCTTAAACAATGCCCTT+AGG - Chr2:71994240-71994259 MsG0280010596.01.T01:intron 40.0%
GGCTAAGAGTTCCTTCAAAT+TGG - Chr2:71994782-71994801 MsG0280010596.01.T01:intron 40.0%
GGGTGTAGGTTATTGTTGAA+AGG - Chr2:71995806-71995825 MsG0280010596.01.T01:intron 40.0%
TATAGTATGCCCCCTTACTT+TGG + Chr2:71994081-71994100 None:intergenic 40.0%
TCCTGTATATGACTTACAGC+GGG - Chr2:71997113-71997132 MsG0280010596.01.T01:intron 40.0%
TGGCAATTGGGTAACGTATA+AGG + Chr2:71994551-71994570 None:intergenic 40.0%
TGTGTGTGAGATGAAGACAA+GGG + Chr2:71996648-71996667 None:intergenic 40.0%
TTACGTCGTTCTAGCAACAT+TGG - Chr2:71996607-71996626 MsG0280010596.01.T01:intron 40.0%
TTATGATTCAGAGAGGAGAC+AGG - Chr2:71995653-71995672 MsG0280010596.01.T01:intron 40.0%
TTGGGAAGGATTTGGCAATT+GGG + Chr2:71994563-71994582 None:intergenic 40.0%
! TCTTCTCTTCTCAACCCTTT+TGG - Chr2:71994657-71994676 MsG0280010596.01.T01:intron 40.0%
!! ATATCATCGGTTCGGTTCAT+CGG - Chr2:71994019-71994038 MsG0280010596.01.T01:intron 40.0%
!! CCACTTTTCCTTTAATTGGG+AGG + Chr2:71996674-71996693 None:intergenic 40.0%
!! CTAACCCAAAGTATTGTTGG+AGG + Chr2:71996566-71996585 None:intergenic 40.0%
!! CTGGTTAATCTTTTGGGTGT+AGG - Chr2:71995792-71995811 MsG0280010596.01.T01:intron 40.0%
!! TGCCCTAAGAGCATTGTTTA+AGG - Chr2:71994123-71994142 MsG0280010596.01.T01:intron 40.0%
ACACACACCCTCCCAATTAA+AGG - Chr2:71996663-71996682 MsG0280010596.01.T01:intron 45.0%
ATAGTGCTCCTGAAGACAAG+AGG + Chr2:71993025-71993044 None:intergenic 45.0%
ATGTAGGGGTCTAAGTGGTT+CGG + Chr2:71994365-71994384 None:intergenic 45.0%
CAGGGAATCGAACCAAAGTA+AGG - Chr2:71994066-71994085 MsG0280010596.01.T01:intron 45.0%
CCTGTATATGACTTACAGCG+GGG - Chr2:71997114-71997133 MsG0280010596.01.T01:intron 45.0%
GAGAAAAGAACGAACCAAGG+GGG + Chr2:71994812-71994831 None:intergenic 45.0%
GCATCTCTTGTGTGATTGGT+AGG + Chr2:71993359-71993378 None:intergenic 45.0%
GGAATCGAACCAAAGTAAGG+GGG - Chr2:71994069-71994088 MsG0280010596.01.T01:intron 45.0%
GGGAATCGAACCAAAGTAAG+GGG - Chr2:71994068-71994087 MsG0280010596.01.T01:intron 45.0%
GTCAGCATCTCTTGTGTGAT+TGG + Chr2:71993363-71993382 None:intergenic 45.0%
GTGTGTGTGAGATGAAGACA+AGG + Chr2:71996649-71996668 None:intergenic 45.0%
GTTCCTATCCTTGCAAACCT+TGG - Chr2:71996134-71996153 MsG0280010596.01.T01:intron 45.0%
GTTCCTGCTGATCTAACTGT+AGG - Chr2:71996840-71996859 MsG0280010596.01.T01:intron 45.0%
GTTCTAGCAACATTGGAGGT+TGG - Chr2:71996614-71996633 MsG0280010596.01.T01:intron 45.0%
TGTTGTGGTTCACCTTGTTG+AGG - Chr2:71995945-71995964 MsG0280010596.01.T01:intron 45.0%
TTCAAATTGGAACACCCCCT+TGG - Chr2:71994795-71994814 MsG0280010596.01.T01:intron 45.0%
TTGCGGAAACAAGATTGGGA+AGG + Chr2:71994577-71994596 None:intergenic 45.0%
! GAGTGAGGATATCATCGGTT+CGG - Chr2:71994011-71994030 MsG0280010596.01.T01:intron 45.0%
! GCTTCTGATGATGTGACTTC+TGG - Chr2:71992998-71993017 MsG0280010596.01.T01:three_prime_UTR 45.0%
! TACTTGGACTAGAGCCATAG+TGG - Chr2:71996019-71996038 MsG0280010596.01.T01:intron 45.0%
!! AAGGGGGTGTTCCAATTTGA+AGG + Chr2:71994796-71994815 None:intergenic 45.0%
!! CTTCAGGAGCACTATTCTTC+AGG - Chr2:71993030-71993049 MsG0280010596.01.T01:three_prime_UTR 45.0%
!! TTCAGGAGCGCTATTTCTTC+AGG - Chr2:71993047-71993066 MsG0280010596.01.T01:three_prime_UTR 45.0%
ACGACGAGCGACACACATTT+CGG + Chr2:71995130-71995149 None:intergenic 50.0%
ATTGTGACAGCGCCTCACAT+TGG - Chr2:71995317-71995336 MsG0280010596.01.T01:intron 50.0%
CCCCGCTGTAAGTCATATAC+AGG + Chr2:71997117-71997136 None:intergenic 50.0%
CGTCGTTCTAGCAACATTGG+AGG - Chr2:71996610-71996629 MsG0280010596.01.T01:intron 50.0%
CTTCTGGACCTCTTGTCTTC+AGG - Chr2:71993014-71993033 MsG0280010596.01.T01:three_prime_UTR 50.0%
GTTGATATCGCAGTCCATGC+AGG - Chr2:71996759-71996778 MsG0280010596.01.T01:intron 50.0%
TGAAGCTTGGAAGCTCCTGA+AGG + Chr2:71993116-71993135 None:intergenic 50.0%
TGGCCTACAGTTAGATCAGC+AGG + Chr2:71996846-71996865 None:intergenic 50.0%
TGTAGGCCAGTTCGTGTATG+TGG - Chr2:71996857-71996876 MsG0280010596.01.T01:intron 50.0%
TTCAGGAGCAAGTTGCCTTC+AGG - Chr2:71993098-71993117 MsG0280010596.01.T01:CDS 50.0%
TTCAGGAGCAGCTTGACTTC+AGG - Chr2:71993064-71993083 MsG0280010596.01.T01:exon 50.0%
TTCATGTGACAACAGCCACG+TGG + Chr2:71993429-71993448 None:intergenic 50.0%
! ACAGCGGGGAGAACACTTTT+GGG - Chr2:71997128-71997147 MsG0280010596.01.T01:intron 50.0%
! TACAGCGGGGAGAACACTTT+TGG - Chr2:71997127-71997146 MsG0280010596.01.T01:intron 50.0%
AGGCTGAAGCTGCTCGCATA+AGG - Chr2:71995673-71995692 MsG0280010596.01.T01:intron 55.0%
GATATCGCAGTCCATGCAGG+TGG - Chr2:71996762-71996781 MsG0280010596.01.T01:intron 55.0%
GCCAGTTCGTGTATGTGGTG+CGG - Chr2:71996862-71996881 MsG0280010596.01.T01:intron 55.0%
GGCTGAAGCTGCTCGCATAA+GGG - Chr2:71995674-71995693 MsG0280010596.01.T01:intron 55.0%
GTCGAACGGCACATCATCGT+AGG - Chr2:71995228-71995247 MsG0280010596.01.T01:intron 55.0%
TCCGCACCACATACACGAAC+TGG + Chr2:71996866-71996885 None:intergenic 55.0%
GTGACAGCGCCTCACATTGG+AGG - Chr2:71995320-71995339 MsG0280010596.01.T01:intron 60.0%
!! GTGATTGGTAGGGGTGCTCG+CGG + Chr2:71993348-71993367 None:intergenic 60.0%
!! GCTCGCGGTGCGGTTTGGTT+CGG + Chr2:71993333-71993352 None:intergenic 65.0%
!! TGGTAGGGGTGCTCGCGGTG+CGG + Chr2:71993343-71993362 None:intergenic 70.0%
! GGGGTGCTCGCGGTGCGGTT+TGG + Chr2:71993338-71993357 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 71992911 71997319 71992911 ID=MsG0280010596.01;Name=MsG0280010596.01
Chr2 mRNA 71992911 71997319 71992911 ID=MsG0280010596.01.T01;Parent=MsG0280010596.01;Name=MsG0280010596.01.T01;_AED=0.58;_eAED=0.58;_QI=0|0.66|0.75|0.75|1|1|4|157|122
Chr2 exon 71992911 71993165 71992911 ID=MsG0280010596.01.T01:exon:19524;Parent=MsG0280010596.01.T01
Chr2 exon 71993279 71993397 71993279 ID=MsG0280010596.01.T01:exon:19523;Parent=MsG0280010596.01.T01
Chr2 exon 71994364 71994416 71994364 ID=MsG0280010596.01.T01:exon:19522;Parent=MsG0280010596.01.T01
Chr2 exon 71997221 71997319 71997221 ID=MsG0280010596.01.T01:exon:19521;Parent=MsG0280010596.01.T01
Chr2 CDS 71997221 71997319 71997221 ID=MsG0280010596.01.T01:cds;Parent=MsG0280010596.01.T01
Chr2 CDS 71994364 71994416 71994364 ID=MsG0280010596.01.T01:cds;Parent=MsG0280010596.01.T01
Chr2 CDS 71993279 71993397 71993279 ID=MsG0280010596.01.T01:cds;Parent=MsG0280010596.01.T01
Chr2 CDS 71993068 71993165 71993068 ID=MsG0280010596.01.T01:cds;Parent=MsG0280010596.01.T01
Chr2 three_prime_UTR 71992911 71993067 71992911 ID=MsG0280010596.01.T01:three_prime_utr;Parent=MsG0280010596.01.T01
Gene Sequence

>MsG0280010596.01.T01

ATGGCTTCTGATGAGGCAGCTTCTGATGATGCAGCTTCTGATGATGCAGCTTCTGATGAGATGACTTCTGCAGCTTCTGATGATGCAGCTTCTGATGATGTTATTGTTGAAAGGGCTGAGAAGAGTGATGTTCCAGATATTGACAAGAAAAAGTATCTGGTTCCTGCTGATCTAACTGTAGGCCAGTTCGTGTATGTGGTGCGGAAGAGAATCAAATTGAGTCCCGAGAAGGCCATCTTCATTTTTGTGAAGAACATTTTACCACCAACCGCTGCTATGATGTCTGCAATATATGAGGAAAACAAGGATGAAGATGGATTCCTGTATATGACTTACAGCGGGGAGAACACTTTTGGGATGTTAATGTGA
Protein sequence

>MsG0280010596.01.T01

MASDEAASDDAASDDAASDEMTSAASDDAASDDVIVERAEKSDVPDIDKKKYLVPADLTVGQFVYVVRKRIKLSPEKAIFIFVKNILPPTAAMMSAIYEENKDEDGFLYMTYSGENTFGMLM*