AlfalfaGEDB | MsG0480022947.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022947.01

KEGG: -
GO: -
PFAM: PF02493

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022947.01.T01	KEH20623.1	61.092	293	93	3	7	299	13	284	3.23E-107	325

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022947.01.T01	Q8RY89	42.512	207	101	1	10	216	7	195	1.17E-43	163

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022947.01.T01	A0A072TUU3	61.092	293	93	3	7	299	13	284	1.54e-107	325

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000470.01	MsG0480022947.01	0.828505	8.660627e-55	8.625438e-52
MsG0180004765.01	MsG0480022947.01	0.816535	5.261451e-52	3.737920e-49
MsG0280007955.01	MsG0480022947.01	0.829917	3.936253e-55	4.085417e-52
MsG0280008112.01	MsG0480022947.01	0.816644	4.973812e-52	3.544190e-49
MsG0280010722.01	MsG0480022947.01	0.809880	1.520286e-50	9.031255e-48
MsG0380011609.01	MsG0480022947.01	0.805485	1.304858e-49	6.911796e-47
MsG0380014468.01	MsG0480022947.01	0.802320	5.933957e-49	2.898214e-46
MsG0480018653.01	MsG0480022947.01	0.820089	8.246860e-53	6.463182e-50
MsG0480020887.01	MsG0480022947.01	0.803529	3.338346e-49	1.681602e-46
MsG0480021869.01	MsG0480022947.01	0.801968	7.010941e-49	3.393233e-46

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022947.01.T01	MTR_8g080860	61.092	293	93	3	7	299	13	284	3.91e-111	325
MsG0480022947.01.T01	MTR_8g080860	61.092	293	93	3	7	299	9	280	4.79e-111	325
MsG0480022947.01.T01	MTR_8g095240	53.401	294	120	5	13	290	3	295	1.46e-83	259
MsG0480022947.01.T01	MTR_8g095240	46.575	292	105	3	14	272	50	323	1.04e-67	219
MsG0480022947.01.T01	MTR_8g095240	46.825	252	117	4	57	292	1	251	1.68e-52	179
MsG0480022947.01.T01	MTR_8g095240	50.000	220	94	4	13	216	118	337	8.63e-52	177
MsG0480022947.01.T01	MTR_8g095240	50.667	150	73	1	12	160	173	322	6.64e-37	137
MsG0480022947.01.T01	MTR_8g092090	49.242	264	100	3	8	271	3	232	8.48e-74	228
MsG0480022947.01.T01	MTR_8g092090	47.660	235	94	4	76	302	2	215	2.50e-56	184
MsG0480022947.01.T01	MTR_8g080310	68.000	175	38	1	51	225	1	157	1.60e-71	219

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022947.01.T01	AT1G60890	41.784	213	106	1	4	216	13	207	1.02e-44	163
MsG0480022947.01.T01	AT1G60890	42.353	170	92	2	128	292	22	190	2.33e-32	128
MsG0480022947.01.T01	AT1G60890	36.224	196	120	1	61	251	24	219	1.22e-29	120
MsG0480022947.01.T01	AT1G60890	35.294	187	115	2	105	286	22	207	6.41e-26	109
MsG0480022947.01.T01	AT1G60890	42.512	207	101	1	10	216	7	195	1.20e-44	163
MsG0480022947.01.T01	AT1G60890	42.353	170	92	2	128	292	10	178	2.70e-32	127
MsG0480022947.01.T01	AT1G60890	35.859	198	122	1	59	251	10	207	1.42e-29	120
MsG0480022947.01.T01	AT1G60890	35.294	187	115	2	105	286	10	195	7.18e-26	108
MsG0480022947.01.T01	AT1G60890	43.434	99	55	1	11	109	100	197	8.70e-18	85.1
MsG0480022947.01.T01	AT1G10900	37.238	239	128	3	10	244	7	227	5.57e-40	150

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Find 83 sgRNAs with CRISPR-Local

Find 199 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGGAAAAGGGATGGAAA+AGG	0.451894	4:-78953454	MsG0480022947.01.T01:CDS
AAAGGGAAAAGGGATGGAAA+AGG	0.451894	4:-78953872	MsG0480022947.01.T01:CDS
AAAGGGAAAATAGAAGGAAA+AGG	0.457356	4:-78954010	MsG0480022947.01.T01:CDS
AAAGGGAACATGGATGGAAA+AGG	0.445128	4:-78953733	MsG0480022947.01.T01:intron
AACAATATATGAGGGCGATT+GGG	0.469878	4:-78954173	MsG0480022947.01.T01:CDS
AAGGAAAAGGGATTATGAAT+TGG	0.317461	4:-78953997	MsG0480022947.01.T01:CDS
AAGGGAAAAGGGATGGAAAA+GGG	0.459912	4:-78953453	MsG0480022947.01.T01:CDS
AAGGGAAAAGGGATGGAAAA+GGG	0.459912	4:-78953871	MsG0480022947.01.T01:CDS
AAGGGAAAATAGAAGGAAAA+GGG	0.480073	4:-78954009	MsG0480022947.01.T01:CDS
AAGGGAACATGGATGGAAAA+GGG	0.408341	4:-78953732	MsG0480022947.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGGGAAAATAGAAGGAAA+AGG	-	Chr4:78952006-78952025	MsG0480022947.01.T01:intron	30.0%
AAGGAAAAGGGATTATGAAT+TGG	-	Chr4:78952019-78952038	MsG0480022947.01.T01:intron	30.0%
AAGGGAAAATAGAAGGAAAA+GGG	-	Chr4:78952007-78952026	MsG0480022947.01.T01:intron	30.0%
AATATGAAAAAGGATGGGAA+TGG	-	Chr4:78952421-78952440	MsG0480022947.01.T01:intron	30.0%
AATTGGAAAAAAAGGGAACA+TGG	-	Chr4:78952273-78952292	MsG0480022947.01.T01:intron	30.0%
ACATTGGAAAAAAGGGAAAA+GGG	-	Chr4:78952134-78952153	MsG0480022947.01.T01:intron	30.0%
ACATTGGAGATTGGAAAAAA+GGG	-	Chr4:78952545-78952564	MsG0480022947.01.T01:intron	30.0%
AGATTGGAAAAAAGGGAAAA+GGG	-	Chr4:78952552-78952571	MsG0480022947.01.T01:intron	30.0%
AGGAAAAGGGATTATGAATT+GGG	-	Chr4:78952020-78952039	MsG0480022947.01.T01:intron	30.0%
AGGATAGTAAAGTTGTTATC+AGG	-	Chr4:78953842-78953861	MsG0480022947.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	78951727	78954311	78951727	ID=MsG0480022947.01;Name=MsG0480022947.01
Chr4	mRNA	78951727	78954311	78951727	ID=MsG0480022947.01.T01;Parent=MsG0480022947.01;Name=MsG0480022947.01.T01;_AED=0.49;_eAED=0.56;_QI=0\|0\|0\|1\|1\|1\|3\|0\|342
Chr4	exon	78953837	78954311	78953837	ID=MsG0480022947.01.T01:exon:23555;Parent=MsG0480022947.01.T01
Chr4	exon	78953350	78953751	78953350	ID=MsG0480022947.01.T01:exon:23554;Parent=MsG0480022947.01.T01
Chr4	exon	78951727	78951878	78951727	ID=MsG0480022947.01.T01:exon:23553;Parent=MsG0480022947.01.T01
Chr4	CDS	78953837	78954311	78953837	ID=MsG0480022947.01.T01:cds;Parent=MsG0480022947.01.T01
Chr4	CDS	78953350	78953751	78953350	ID=MsG0480022947.01.T01:cds;Parent=MsG0480022947.01.T01
Chr4	CDS	78951727	78951878	78951727	ID=MsG0480022947.01.T01:cds;Parent=MsG0480022947.01.T01

Gene Sequence

>MsG0480022947.01.T01

ATGTTTCTTGTAAAGTGTAGTTTTAAAGAGAAGTCATTTTCAAACGGGGATGTCTACATTGATAGCTTCAAGGGTGAACTTTGTCATGGCAACGGAAAGTACACATGGTTAAATGGAACAATATATGAGGGCGATTGGGTTGATGGACAAATGACAGGGAAAGGGATTATGAAGTGGGTTAATGGTAGTGTTTTCGATGGTTGTTGGTCAAATGGACTTAAAGATGGATATGGAGTTTATAGATTTGCAAATGGGGATGTTTATAGAGGAAATTGGGAAAAAGGGAAAATAGAAGGAAAAGGGATTATGAATTGGGCAAGTGGTTCTGTTTTCGATGGTTGTTGGTCAAATGGACTTAGAGATGGATATGGAGTTTATAGATTTGCAAATGGGGATGTCCACACTGGACATTGGAAAAAAGGGAAAAGGGATGGAAAAGGGATTATGAGATGGGCTAATGTTGATGTTTTCGATGGGAACATGGATGGAAAAGGGGTTATGAATTGGGCTAACGGTGATGTTTTTGATGGTTGTTGGTTAAATGGAGTTAGACATGGATCTGGAATTTATACAAATGCAAACGGAGATGTCTTTACTGGAAAATGGAAAAATATGAAAAAGGATGGGAATGGGATTATGAATTGGGCTAATGGTGATGTTTTTGTTGGTCGTTGGTCAAATGGACTTATACATGGATCTGCTGGAGTTTATAGATTTGCGAATGGGAATGTCTACATTGGAGATTGGAAAAAAGGGAAAAGGGATGGAAAAGGGATTTTGAATAGGGCTAATGGTGATGTATTTGTTGGTTGTTGGTCAAATGGACTTAGACATGGATCTGGAGTTTATAGATTTGCAAACGGGGACTTGTACTTTGAGATCATGACGATGAGAAAGGCTGATCTCATATACTTCATACTGAACACTACTCATGGACATGATGATGATGATGATGATGATGATGATGATGATGATGATGGGAGGGCCATTTGGTATAGAAGATATGAAGATGTACAGTCAGGAATTTAG

Protein sequence

>MsG0480022947.01.T01

MFLVKCSFKEKSFSNGDVYIDSFKGELCHGNGKYTWLNGTIYEGDWVDGQMTGKGIMKWVNGSVFDGCWSNGLKDGYGVYRFANGDVYRGNWEKGKIEGKGIMNWASGSVFDGCWSNGLRDGYGVYRFANGDVHTGHWKKGKRDGKGIMRWANVDVFDGNMDGKGVMNWANGDVFDGCWLNGVRHGSGIYTNANGDVFTGKWKNMKKDGNGIMNWANGDVFVGRWSNGLIHGSAGVYRFANGNVYIGDWKKGKRDGKGILNRANGDVFVGCWSNGLRHGSGVYRFANGDLYFEIMTMRKADLIYFILNTTHGHDDDDDDDDDDDDDGRAIWYRRYEDVQSGI*