Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023383.01.T01 | KEH20878.1 | 89.062 | 128 | 6 | 1 | 1 | 120 | 1 | 128 | 1.44E-75 | 230 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023383.01.T01 | Q93WU9 | 34.375 | 128 | 71 | 3 | 6 | 120 | 3 | 130 | 1.78E-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023383.01.T01 | A0A072TVK6 | 89.062 | 128 | 6 | 1 | 1 | 120 | 1 | 128 | 6.88e-76 | 230 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0480023383.01.T01 | TF | WRKY |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000525.01 | MsG0480023383.01 | 0.806502 | 7.975883e-50 | 4.336091e-47 |
| MsG0180004766.01 | MsG0480023383.01 | 0.807375 | 5.212599e-50 | 2.898574e-47 |
| MsG0480023383.01 | MsG0480023898.01 | 0.800492 | 1.405076e-48 | 6.551455e-46 |
| MsG0480023383.01 | MsG0480023900.01 | 0.804169 | 2.458174e-49 | 1.258683e-46 |
| MsG0380016708.01 | MsG0480023383.01 | 0.829586 | 4.738260e-55 | 4.870498e-52 |
| MsG0280006839.01 | MsG0480023383.01 | 0.800279 | 1.552809e-48 | 7.201296e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023383.01.T01 | MTR_8g092010 | 89.062 | 128 | 6 | 1 | 1 | 120 | 1 | 128 | 1.74e-79 | 230 |
| MsG0480023383.01.T01 | MTR_8g092010 | 89.062 | 128 | 6 | 1 | 1 | 120 | 1 | 128 | 1.02e-78 | 230 |
| MsG0480023383.01.T01 | MTR_3g095040 | 48.227 | 141 | 49 | 8 | 1 | 120 | 1 | 138 | 2.20e-24 | 92.8 |
| MsG0480023383.01.T01 | MTR_1g015140 | 37.391 | 115 | 56 | 3 | 13 | 120 | 17 | 122 | 4.60e-14 | 64.7 |
| MsG0480023383.01.T01 | MTR_3g104750 | 73.529 | 34 | 9 | 0 | 87 | 120 | 91 | 124 | 7.93e-13 | 61.6 |
| MsG0480023383.01.T01 | MTR_5g029850 | 50.877 | 57 | 22 | 1 | 67 | 117 | 12 | 68 | 1.72e-12 | 58.5 |
| MsG0480023383.01.T01 | MTR_4g013260 | 33.333 | 93 | 60 | 1 | 28 | 120 | 266 | 356 | 2.41e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480023383.01.T01 | AT5G64810 | 34.375 | 128 | 71 | 3 | 6 | 120 | 3 | 130 | 1.82e-13 | 63.9 |
| MsG0480023383.01.T01 | AT5G26170 | 70.588 | 34 | 10 | 0 | 87 | 120 | 100 | 133 | 1.02e-12 | 61.6 |
| MsG0480023383.01.T01 | AT2G46130 | 60.526 | 38 | 15 | 0 | 79 | 116 | 9 | 46 | 2.08e-11 | 56.6 |
| MsG0480023383.01.T01 | AT2G46130 | 60.526 | 38 | 15 | 0 | 79 | 116 | 9 | 46 | 2.48e-11 | 56.6 |
Find 28 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGTAGAGTGAGCAAAATT+TGG | 0.273680 | 4:+84486527 | None:intergenic |
| AAGATCAGAGCTTGAGATTA+TGG | 0.274000 | 4:-84485889 | MsG0480023383.01.T01:CDS |
| ATGGGTTGTTGGTATATAAA+TGG | 0.277706 | 4:+84486602 | None:intergenic |
| CTTGTTCTAAAGGCTATCTT+TGG | 0.312484 | 4:+84485909 | None:intergenic |
| TGCATAGATAAACTCAGTTA+TGG | 0.351269 | 4:+84486583 | None:intergenic |
| TGCATCATTGAATTCTTGAT+TGG | 0.365316 | 4:+84486391 | None:intergenic |
| AAATGGGATTAAGCGAAATA+AGG | 0.381517 | 4:-84485943 | MsG0480023383.01.T01:CDS |
| TTACATATTATTGTTCTTTG+AGG | 0.400718 | 4:+84486367 | None:intergenic |
| TTGCTTGATGATATTTGTAT+TGG | 0.407278 | 4:-84486479 | MsG0480023383.01.T01:CDS |
| GCATAGATAAACTCAGTTAT+GGG | 0.408805 | 4:+84486584 | None:intergenic |
| TAAACTCAGTTATGGGTTGT+TGG | 0.446707 | 4:+84486591 | None:intergenic |
| TGTTACCTTCTCTAATGATT+CGG | 0.455426 | 4:+84486424 | None:intergenic |
| GGATACAAGTGGAGGAAGTA+CGG | 0.474552 | 4:-84485861 | MsG0480023383.01.T01:CDS |
| AAGCTCTGATCTTGTTCTAA+AGG | 0.476991 | 4:+84485899 | None:intergenic |
| GAGCTTGAGATTATGGATGA+TGG | 0.492012 | 4:-84485882 | MsG0480023383.01.T01:CDS |
| GTGACCGAGATTCTTGATCA+AGG | 0.501478 | 4:+84486453 | None:intergenic |
| AAGCGAAATAAGGTAGAAGC+GGG | 0.536791 | 4:-84485933 | MsG0480023383.01.T01:CDS |
| AACTGAGTTTATCTATGCAA+TGG | 0.539741 | 4:-84486579 | MsG0480023383.01.T01:exon |
| CAAAATTTGGAGTAGCATGA+GGG | 0.551934 | 4:+84486540 | None:intergenic |
| GATACAAGTGGAGGAAGTAC+GGG | 0.554483 | 4:-84485860 | MsG0480023383.01.T01:CDS |
| TGAAGAACAGTCCTAACCTA+AGG | 0.563814 | 4:-84485827 | MsG0480023383.01.T01:CDS |
| GTCACCTTGATCAAGAATCT+CGG | 0.568789 | 4:-84486457 | MsG0480023383.01.T01:CDS |
| GCAAAATTTGGAGTAGCATG+AGG | 0.589246 | 4:+84486539 | None:intergenic |
| TTATGGATGATGGATACAAG+TGG | 0.599156 | 4:-84485872 | MsG0480023383.01.T01:CDS |
| AAGTACCGAATCATTAGAGA+AGG | 0.606756 | 4:-84486429 | MsG0480023383.01.T01:CDS |
| TAAGCGAAATAAGGTAGAAG+CGG | 0.616655 | 4:-84485934 | MsG0480023383.01.T01:CDS |
| TGGATGATGGATACAAGTGG+AGG | 0.653822 | 4:-84485869 | MsG0480023383.01.T01:CDS |
| AGCGAAATAAGGTAGAAGCG+GGG | 0.727454 | 4:-84485932 | MsG0480023383.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTCATTTAAAATTAAGAAAA+GGG | - | Chr4:84486150-84486169 | MsG0480023383.01.T01:intron | 10.0% |
| !!! | GTTCATTTAAAATTAAGAAA+AGG | - | Chr4:84486149-84486168 | MsG0480023383.01.T01:intron | 15.0% |
| !! | TTACATATTATTGTTCTTTG+AGG | + | Chr4:84486099-84486118 | None:intergenic | 20.0% |
| !! | TTTGCAGAAAATACAAAAAT+GGG | - | Chr4:84486504-84486523 | MsG0480023383.01.T01:CDS | 20.0% |
| !!! | CTAATAAAGTGTACATATTT+TGG | - | Chr4:84486463-84486482 | MsG0480023383.01.T01:CDS | 20.0% |
| !!! | TAAAAAACATCATTTTGTTC+AGG | + | Chr4:84486259-84486278 | None:intergenic | 20.0% |
| !!! | TTTTGCAGAAAATACAAAAA+TGG | - | Chr4:84486503-84486522 | MsG0480023383.01.T01:CDS | 20.0% |
| ! | AACAAGAGCAATACAAATTA+AGG | + | Chr4:84486132-84486151 | None:intergenic | 25.0% |
| ! | ACAAGAGCAATACAAATTAA+GGG | + | Chr4:84486131-84486150 | None:intergenic | 25.0% |
| ! | ATTAATGAAGCATATACCAT+CGG | - | Chr4:84486394-84486413 | MsG0480023383.01.T01:CDS | 25.0% |
| !! | TCAAAAGTGTACTTCTAAAT+GGG | + | Chr4:84486212-84486231 | None:intergenic | 25.0% |
| !! | TTCAAAAGTGTACTTCTAAA+TGG | + | Chr4:84486213-84486232 | None:intergenic | 25.0% |
| !! | TTGCTTGATGATATTTGTAT+TGG | - | Chr4:84485984-84486003 | MsG0480023383.01.T01:intron | 25.0% |
| !!! | TCTAAAAGTGTACATTTTGA+GGG | - | Chr4:84486282-84486301 | MsG0480023383.01.T01:intron | 25.0% |
| AAATGGGATTAAGCGAAATA+AGG | - | Chr4:84486520-84486539 | MsG0480023383.01.T01:CDS | 30.0% | |
| AACTGAGTTTATCTATGCAA+TGG | - | Chr4:84485884-84485903 | MsG0480023383.01.T01:CDS | 30.0% | |
| GCATAGATAAACTCAGTTAT+GGG | + | Chr4:84485882-84485901 | None:intergenic | 30.0% | |
| TGCATAGATAAACTCAGTTA+TGG | + | Chr4:84485883-84485902 | None:intergenic | 30.0% | |
| TGCATCATTGAATTCTTGAT+TGG | + | Chr4:84486075-84486094 | None:intergenic | 30.0% | |
| TGTTACCTTCTCTAATGATT+CGG | + | Chr4:84486042-84486061 | None:intergenic | 30.0% | |
| ! | ATGGGTTGTTGGTATATAAA+TGG | + | Chr4:84485864-84485883 | None:intergenic | 30.0% |
| !! | AGTGTACATATTTTGGATTG+TGG | - | Chr4:84486470-84486489 | MsG0480023383.01.T01:CDS | 30.0% |
| !! | ATCGGTTTTTAAGAAACTAC+GGG | + | Chr4:84486189-84486208 | None:intergenic | 30.0% |
| !! | GATCGGTTTTTAAGAAACTA+CGG | + | Chr4:84486190-84486209 | None:intergenic | 30.0% |
| !! | GTCTAAAAGTGTACATTTTG+AGG | - | Chr4:84486281-84486300 | MsG0480023383.01.T01:intron | 30.0% |
| !! | GTGTACATATTTTGGATTGT+GGG | - | Chr4:84486471-84486490 | MsG0480023383.01.T01:CDS | 30.0% |
| !! | TGTACATATTTTGGATTGTG+GGG | - | Chr4:84486472-84486491 | MsG0480023383.01.T01:CDS | 30.0% |
| AAGATCAGAGCTTGAGATTA+TGG | - | Chr4:84486574-84486593 | MsG0480023383.01.T01:exon | 35.0% | |
| AGTGTACTTCTAAATGGGAT+CGG | + | Chr4:84486207-84486226 | None:intergenic | 35.0% | |
| CAAATGAAGTAATGAACCGA+TGG | + | Chr4:84486413-84486432 | None:intergenic | 35.0% | |
| GAAGTAGAGTGAGCAAAATT+TGG | + | Chr4:84485939-84485958 | None:intergenic | 35.0% | |
| GCAATACAAATTAAGGGAGA+AGG | + | Chr4:84486125-84486144 | None:intergenic | 35.0% | |
| TAAGCGAAATAAGGTAGAAG+CGG | - | Chr4:84486529-84486548 | MsG0480023383.01.T01:CDS | 35.0% | |
| ! | AAGCTCTGATCTTGTTCTAA+AGG | + | Chr4:84486567-84486586 | None:intergenic | 35.0% |
| ! | CAAAATTTGGAGTAGCATGA+GGG | + | Chr4:84485926-84485945 | None:intergenic | 35.0% |
| ! | TAAACTCAGTTATGGGTTGT+TGG | + | Chr4:84485875-84485894 | None:intergenic | 35.0% |
| !! | AAGTACCGAATCATTAGAGA+AGG | - | Chr4:84486034-84486053 | MsG0480023383.01.T01:intron | 35.0% |
| !! | AAGTGTACATTTTGAGGGAT+CGG | - | Chr4:84486287-84486306 | MsG0480023383.01.T01:intron | 35.0% |
| !! | CTTGTTCTAAAGGCTATCTT+TGG | + | Chr4:84486557-84486576 | None:intergenic | 35.0% |
| !! | GGATCGGTTTTTAGAAATCA+TGG | - | Chr4:84486303-84486322 | MsG0480023383.01.T01:intron | 35.0% |
| !! | TCGGTTTTTAAGAAACTACG+GGG | + | Chr4:84486188-84486207 | None:intergenic | 35.0% |
| !! | TTATGGATGATGGATACAAG+TGG | - | Chr4:84486591-84486610 | MsG0480023383.01.T01:five_prime_UTR | 35.0% |
| !!! | TGCATGATTGCGTTTTTTCA+AGG | + | Chr4:84486343-84486362 | None:intergenic | 35.0% |
| AAGCGAAATAAGGTAGAAGC+GGG | - | Chr4:84486530-84486549 | MsG0480023383.01.T01:CDS | 40.0% | |
| GAGCTTGAGATTATGGATGA+TGG | - | Chr4:84486581-84486600 | MsG0480023383.01.T01:exon | 40.0% | |
| GTCACCTTGATCAAGAATCT+CGG | - | Chr4:84486006-84486025 | MsG0480023383.01.T01:intron | 40.0% | |
| TGAAGAACAGTCCTAACCTA+AGG | - | Chr4:84486636-84486655 | MsG0480023383.01.T01:five_prime_UTR | 40.0% | |
| ! | GCAAAATTTGGAGTAGCATG+AGG | + | Chr4:84485927-84485946 | None:intergenic | 40.0% |
| AGCGAAATAAGGTAGAAGCG+GGG | - | Chr4:84486531-84486550 | MsG0480023383.01.T01:CDS | 45.0% | |
| GGATACAAGTGGAGGAAGTA+CGG | - | Chr4:84486602-84486621 | MsG0480023383.01.T01:five_prime_UTR | 45.0% | |
| GTGACCGAGATTCTTGATCA+AGG | + | Chr4:84486013-84486032 | None:intergenic | 45.0% | |
| ! | GATACAAGTGGAGGAAGTAC+GGG | - | Chr4:84486603-84486622 | MsG0480023383.01.T01:five_prime_UTR | 45.0% |
| !! | TGGATGATGGATACAAGTGG+AGG | - | Chr4:84486594-84486613 | MsG0480023383.01.T01:five_prime_UTR | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 84485824 | 84486661 | 84485824 | ID=MsG0480023383.01;Name=MsG0480023383.01 |
| Chr4 | mRNA | 84485824 | 84486661 | 84485824 | ID=MsG0480023383.01.T01;Parent=MsG0480023383.01;Name=MsG0480023383.01.T01;_AED=0.40;_eAED=0.41;_QI=79|1|0.5|1|1|1|2|0|120 |
| Chr4 | exon | 84485824 | 84485974 | 84485824 | ID=MsG0480023383.01.T01:exon:2768;Parent=MsG0480023383.01.T01 |
| Chr4 | exon | 84486371 | 84486661 | 84486371 | ID=MsG0480023383.01.T01:exon:2767;Parent=MsG0480023383.01.T01 |
| Chr4 | five_prime_UTR | 84486583 | 84486661 | 84486583 | ID=MsG0480023383.01.T01:five_prime_utr;Parent=MsG0480023383.01.T01 |
| Chr4 | CDS | 84486371 | 84486582 | 84486371 | ID=MsG0480023383.01.T01:cds;Parent=MsG0480023383.01.T01 |
| Chr4 | CDS | 84485824 | 84485974 | 84485824 | ID=MsG0480023383.01.T01:cds;Parent=MsG0480023383.01.T01 |
| Gene Sequence |
| Protein sequence |
