AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023898.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023898.01.T01 MTR_8g104490 79.115 407 28 3 13 363 17 422 0.0 628
MsG0480023898.01.T01 MTR_8g104490 75.306 409 40 4 13 363 82 487 0.0 596
MsG0480023898.01.T01 MTR_8g104510 68.687 396 61 6 13 349 82 473 2.92e-176 498
MsG0480023898.01.T01 MTR_6g083730 50.614 407 133 11 8 354 77 475 9.66e-120 355
MsG0480023898.01.T01 MTR_6g083320 51.397 358 138 13 8 354 77 409 6.74e-103 310
MsG0480023898.01.T01 MTR_2g011810 32.095 296 140 5 24 260 97 390 1.04e-49 176
MsG0480023898.01.T01 MTR_4g090970 44.767 172 93 2 4 175 77 246 2.00e-45 164
MsG0480023898.01.T01 MTR_4g128600 48.684 152 77 1 24 175 94 244 3.38e-45 164
MsG0480023898.01.T01 MTR_2g450080 44.118 170 93 2 6 175 78 245 4.23e-43 158
MsG0480023898.01.T01 MTR_4g106810 46.795 156 80 2 20 175 79 231 6.17e-42 154
MsG0480023898.01.T01 MTR_7g112000 41.401 157 83 3 21 175 80 229 2.94e-26 110
MsG0480023898.01.T01 MTR_7g111960 37.500 168 93 4 24 184 101 263 4.84e-21 94.7
MsG0480023898.01.T01 MTR_7g111980 40.909 154 85 3 24 176 10 158 5.20e-21 94.4
MsG0480023898.01.T01 MTR_7g111980 41.176 153 84 3 24 175 93 240 5.78e-21 94.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023898.01.T01 AT1G73805 36.735 343 150 15 13 296 78 412 4.02e-52 179
MsG0480023898.01.T01 AT4G25800 33.784 296 135 5 24 260 83 376 3.37e-49 174
MsG0480023898.01.T01 AT4G25800 33.784 296 135 5 24 260 83 376 3.37e-49 174
MsG0480023898.01.T01 AT5G57580 51.316 152 73 1 24 175 94 244 3.48e-46 167
MsG0480023898.01.T01 AT2G18750 46.053 152 81 1 24 175 98 248 3.97e-44 161
MsG0480023898.01.T01 AT2G18750 46.053 152 81 1 24 175 98 248 3.97e-44 161
MsG0480023898.01.T01 AT2G18750 46.053 152 81 1 24 175 98 248 3.97e-44 161
MsG0480023898.01.T01 AT5G62570 47.436 156 78 2 20 175 82 233 3.75e-38 139
MsG0480023898.01.T01 AT2G24300 44.737 152 83 1 24 175 42 192 9.26e-38 142
MsG0480023898.01.T01 AT5G62570 47.436 156 78 2 20 175 82 233 1.17e-37 139
MsG0480023898.01.T01 AT2G24300 44.737 152 83 1 24 175 93 243 1.47e-37 142
MsG0480023898.01.T01 AT2G24300 44.737 152 83 1 24 175 89 239 1.63e-37 142
MsG0480023898.01.T01 AT5G62570 47.436 156 78 2 20 175 67 218 1.89e-37 141
MsG0480023898.01.T01 AT5G62570 47.436 156 78 2 20 175 82 233 2.77e-37 140
MsG0480023898.01.T01 AT4G31000 32.117 274 145 7 24 258 88 359 8.89e-37 140
MsG0480023898.01.T01 AT4G31000 44.737 152 79 2 24 175 93 239 6.16e-36 137
MsG0480023898.01.T01 AT5G26920 30.690 290 134 9 24 261 86 360 3.98e-32 127
MsG0480023898.01.T01 AT5G26920 42.857 154 78 4 24 176 86 230 2.49e-28 114
MsG0480023898.01.T01 AT5G26920 29.195 298 136 10 24 261 86 368 7.39e-28 115

Find 105 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCAAATGGATGATAAAT+TGG 0.135753 4:+91024411 None:intergenic
TATGATCAAAACAACTATTT+TGG 0.186437 4:-91023918 MsG0480023898.01.T01:CDS
AACATGAATTGAAAACTTAA+TGG 0.190103 4:+91025906 None:intergenic
TATTTGACGCAACCTTAAAA+TGG 0.197207 4:+91023561 None:intergenic
GTCCTAAATTCTTTCCAAAA+TGG 0.220867 4:-91026579 MsG0480023898.01.T01:five_prime_UTR
GTAGACTTGTAGGGACTATT+TGG 0.242899 4:+91025787 None:intergenic
AACAATAACAATGGACAAAT+AGG 0.309111 4:-91023864 MsG0480023898.01.T01:CDS
ACTACAATTTCTATCTTGAT+TGG 0.325646 4:+91025759 None:intergenic
ATAGAAATTGTAGTTCTTGA+TGG 0.327443 4:-91025750 MsG0480023898.01.T01:CDS
AATTCATGTGACAGGGTAAT+AGG 0.329260 4:-91026279 MsG0480023898.01.T01:intron
ATTCTACTACCGGTGAATAT+TGG 0.346627 4:+91025864 None:intergenic
GTTCTCATGCGTTTGTCGTT+TGG 0.353704 4:+91026513 None:intergenic
ATTCAAATGGATGATAAATT+GGG 0.356032 4:+91024412 None:intergenic
CATGCAAAGACTTGTGTAAT+GGG 0.357437 4:-91024461 MsG0480023898.01.T01:CDS
GGGAAAAGACCATTGCTTAC+AGG 0.362337 4:-91025651 MsG0480023898.01.T01:CDS
TGTGAATCTAAGAAGCTATA+AGG 0.365716 4:+91023789 None:intergenic
GATCTAACGCCTTCAAGTTC+AGG 0.368265 4:-91023753 MsG0480023898.01.T01:CDS
AGGGATGGAATTGCACCAAT+TGG 0.372898 4:-91025609 MsG0480023898.01.T01:CDS
GCTTGCTCTGATGTTATTGT+TGG 0.374740 4:+91026604 None:intergenic
ATTGGGTGAGGTAGACTTGT+AGG 0.383658 4:+91025777 None:intergenic
ACGTATCCAGCTAGAATTAT+CGG 0.390207 4:+91025572 None:intergenic
CGGTCATGCCTTCACGAATT+CGG 0.392944 4:+91025496 None:intergenic
TGATGTTAGCTCTAACCATT+TGG 0.400701 4:+91024381 None:intergenic
AATATTGTGAAAGAGAGAAA+TGG 0.401290 4:-91025672 MsG0480023898.01.T01:CDS
CTACAATTTCTATCTTGATT+GGG 0.407874 4:+91025760 None:intergenic
TTTGGCATATTGCATTCAAA+TGG 0.408571 4:+91024399 None:intergenic
TTTAAGGTTGCGTCAAATAT+GGG 0.414284 4:-91023557 MsG0480023898.01.T01:three_prime_UTR
CCCACATCACTATTCATGAA+TGG 0.421363 4:-91023819 MsG0480023898.01.T01:CDS
ATCTTTAACAACAAAAGCTT+CGG 0.424400 4:+91025476 None:intergenic
GGGATGGAATTGCACCAATT+GGG 0.430451 4:-91025608 MsG0480023898.01.T01:CDS
AAAATATAGCGAGTATATCT+TGG 0.434635 4:-91023613 MsG0480023898.01.T01:three_prime_UTR
GAGAATTAAATCTTACTATG+AGG 0.442875 4:-91025629 MsG0480023898.01.T01:CDS
CCACATCACTATTCATGAAT+GGG 0.446633 4:-91023818 MsG0480023898.01.T01:CDS
TGAGAGAAGCATATGCTAGA+TGG 0.455078 4:-91024204 MsG0480023898.01.T01:CDS
ATATTGTGAAAGAGAGAAAT+GGG 0.455511 4:-91025671 MsG0480023898.01.T01:CDS
ATTTGACGCAACCTTAAAAT+GGG 0.463283 4:+91023562 None:intergenic
ACATGCAAAGACTTGTGTAA+TGG 0.464107 4:-91024462 MsG0480023898.01.T01:CDS
CTTGAAGCTCTATCAATGAT+TGG 0.465914 4:+91023729 None:intergenic
TTTGAATGCAATATGCCAAA+TGG 0.472527 4:-91024396 MsG0480023898.01.T01:CDS
CTTACCACTCTTCTAAGTAA+AGG 0.474223 4:+91026207 None:intergenic
AAGGTTCCAAGCCAGAGAAA+AGG 0.490929 4:+91026226 None:intergenic
CGAGACATTAACAACACATT+GGG 0.498962 4:-91024338 MsG0480023898.01.T01:CDS
TGTCTCGGATGGGAATGGTT+TGG 0.499358 4:+91024354 None:intergenic
GGTAATAGGAGAAATTGTTA+TGG 0.512172 4:-91026265 MsG0480023898.01.T01:five_prime_UTR
ATGATTGGTCCTGAACTTGA+AGG 0.512823 4:+91023744 None:intergenic
GTGAACTCAATATCCCCAAT+TGG 0.516335 4:+91025594 None:intergenic
ATGAATAGTGATGTGGGAAA+TGG 0.517878 4:+91023825 None:intergenic
CGGACACTTTGATTAGTCCC+AGG 0.523841 4:+91025516 None:intergenic
TGTTATTGTTGGTGTAAGAA+AGG 0.526174 4:+91026615 None:intergenic
ATTTCTATCTTGATTGGGTG+AGG 0.527373 4:+91025765 None:intergenic
AACGTTGCTTTGTTAACTCA+AGG 0.528420 4:-91024145 MsG0480023898.01.T01:intron
ATGGTTAGAGCTAACATCAA+TGG 0.535787 4:-91024377 MsG0480023898.01.T01:CDS
GGACAAATAGGTTGCATCGA+GGG 0.538004 4:-91023852 MsG0480023898.01.T01:CDS
TTTCAAAGCAATAATCATCA+AGG 0.539152 4:-91023954 MsG0480023898.01.T01:CDS
GTTGCTGTTAGAATTGCTCC+TGG 0.539447 4:-91025534 MsG0480023898.01.T01:CDS
TTTGGTGATACTCTCGAGAC+TGG 0.541346 4:-91023900 MsG0480023898.01.T01:CDS
TTAAATCTTACTATGAGGGA+TGG 0.541822 4:-91025624 MsG0480023898.01.T01:CDS
CATGTCTAGTATTCTACTAC+CGG 0.542523 4:+91025854 None:intergenic
AGTTCACCGATAATTCTAGC+TGG 0.544574 4:-91025578 MsG0480023898.01.T01:CDS
CTTTCACTTCCAATATTCAC+CGG 0.544704 4:-91025873 MsG0480023898.01.T01:CDS
CCAATGTGTTGTTAATGTCT+CGG 0.546259 4:+91024339 None:intergenic
TGCTAGATGGAATGAGTTGG+AGG 0.547803 4:-91024191 MsG0480023898.01.T01:CDS
TTGGAATGTCAGAGAAAATG+TGG 0.547888 4:-91024499 MsG0480023898.01.T01:CDS
TTTAATTCTCCTGTAAGCAA+TGG 0.551769 4:+91025642 None:intergenic
GTTAATGTCTCGGATGGGAA+TGG 0.555065 4:+91024349 None:intergenic
TTGGGTGAGGTAGACTTGTA+GGG 0.558041 4:+91025778 None:intergenic
AGAATTAAATCTTACTATGA+GGG 0.564326 4:-91025628 MsG0480023898.01.T01:CDS
ATTGCTTTGAAATTGATCCA+TGG 0.565221 4:+91023965 None:intergenic
CATTGCTTGAAGTCTCATGA+AGG 0.566569 4:+91025935 None:intergenic
AATGTGATTCTTGTTGACAA+AGG 0.567650 4:-91025816 MsG0480023898.01.T01:CDS
CAATCATTGATAGAGCTTCA+AGG 0.569919 4:-91023728 MsG0480023898.01.T01:CDS
CGGTAGTAGAATACTAGACA+TGG 0.574833 4:-91025853 MsG0480023898.01.T01:CDS
GGATGGAATTGCACCAATTG+GGG 0.580908 4:-91025607 MsG0480023898.01.T01:CDS
GGATGATAAATTGGGGTCCA+CGG 0.583121 4:+91024420 None:intergenic
TATGTATCAAACAATAACAA+TGG 0.588692 4:-91023873 MsG0480023898.01.T01:CDS
CCGAGACATTAACAACACAT+TGG 0.589170 4:-91024339 MsG0480023898.01.T01:CDS
GACAAATAGGTTGCATCGAG+GGG 0.589199 4:-91023851 MsG0480023898.01.T01:CDS
CCATTCATGAATAGTGATGT+GGG 0.592181 4:+91023819 None:intergenic
GGAACCTTTACTTAGAAGAG+TGG 0.595523 4:-91026211 MsG0480023898.01.T01:intron
TTCTTAGATTCACAATCAGA+CGG 0.596172 4:-91023780 MsG0480023898.01.T01:CDS
GGTGACTTTGTTATTGATCG+TGG 0.600634 4:+91025956 None:intergenic
TGTGTTGTTAATGTCTCGGA+TGG 0.608686 4:+91024343 None:intergenic
CCCATTCATGAATAGTGATG+TGG 0.612706 4:+91023818 None:intergenic
ATATGCTAGATGGAATGAGT+TGG 0.618437 4:-91024194 MsG0480023898.01.T01:CDS
CTTCATGAGACTTCAAGCAA+TGG 0.618672 4:-91025934 MsG0480023898.01.T01:CDS
ACAATAACATCAGAGCAAGC+TGG 0.619100 4:-91026601 MsG0480023898.01.T01:five_prime_UTR
GACTTTGTTATTGATCGTGG+TGG 0.621283 4:+91025959 None:intergenic
AGTAGAATACTAGACATGGA+TGG 0.625124 4:-91025849 MsG0480023898.01.T01:CDS
TTCAAATGGATGATAAATTG+GGG 0.625219 4:+91024413 None:intergenic
TTGCTGTTAGAATTGCTCCT+GGG 0.625923 4:-91025533 MsG0480023898.01.T01:CDS
TGATTAGGTGAATGAAGAGG+TGG 0.626231 4:-91026005 MsG0480023898.01.T01:intron
TCACTCTACAGGTGGAACCA+TGG 0.626249 4:-91023982 MsG0480023898.01.T01:intron
CAAAGTGTCCGAATTCGTGA+AGG 0.626320 4:-91025504 MsG0480023898.01.T01:CDS
AATAGTGATGTGGGAAATGG+TGG 0.626558 4:+91023828 None:intergenic
GGAATCATCATGGACTAGTG+AGG 0.638853 4:-91025706 MsG0480023898.01.T01:CDS
CATTAACAACACATTGGGCA+AGG 0.650846 4:-91024333 MsG0480023898.01.T01:intron
AACAAACGTTATGTCTACCG+TGG 0.654103 4:-91024437 MsG0480023898.01.T01:CDS
GTGTAAGAAAGGTTGCAGTG+AGG 0.681868 4:+91026626 None:intergenic
ATATGATTAGGTGAATGAAG+AGG 0.685589 4:-91026008 MsG0480023898.01.T01:intron
TGGACAAATAGGTTGCATCG+AGG 0.690086 4:-91023853 MsG0480023898.01.T01:CDS
TTAGATTCACAATCAGACGG+TGG 0.697439 4:-91023777 MsG0480023898.01.T01:CDS
TGGAATGTCAGAGAAAATGT+GGG 0.706104 4:-91024498 MsG0480023898.01.T01:CDS
GTGTTGTTAATGTCTCGGAT+GGG 0.715548 4:+91024344 None:intergenic
TTTGTTGTTAAAGATCATCG+CGG 0.765249 4:-91025468 MsG0480023898.01.T01:CDS
TCATTGATAGAGCTTCAAGG+TGG 0.769312 4:-91023725 MsG0480023898.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAGTTAATGTTTATA+TGG - Chr4:91025649-91025668 MsG0480023898.01.T01:CDS 10.0%
!! AACATAATAAAATGAATATT+GGG + Chr4:91023761-91023780 None:intergenic 10.0%
!!! TGTTTCTATAAATTTAATTA+AGG + Chr4:91024061-91024080 None:intergenic 10.0%
!!! TTTTTGTTTAGTTTATTTTA+AGG - Chr4:91025151-91025170 MsG0480023898.01.T01:intron 10.0%
!! AGATTGATTATAATATGTAT+AGG - Chr4:91024864-91024883 MsG0480023898.01.T01:intron 15.0%
!! CTATAAATTTAATTAAGGTA+TGG + Chr4:91024056-91024075 None:intergenic 15.0%
!! CTTTCTATTTATATATGATT+AGG - Chr4:91024173-91024192 MsG0480023898.01.T01:CDS 15.0%
!! GAACATAATAAAATGAATAT+TGG + Chr4:91023762-91023781 None:intergenic 15.0%
!! TTAATTTCATTCGAAAATTA+TGG + Chr4:91024028-91024047 None:intergenic 15.0%
!!! TATTTTTATGTTTTCTTTCT+TGG - Chr4:91024904-91024923 MsG0480023898.01.T01:intron 15.0%
!!! TTCAATTAGAAATTTACTTT+TGG + Chr4:91026134-91026153 None:intergenic 15.0%
!! AAAAATCAATGCATAGAAAA+AGG + Chr4:91025923-91025942 None:intergenic 20.0%
!! AAAATTATGGTAAAGTTGAA+TGG + Chr4:91024015-91024034 None:intergenic 20.0%
!! AACATGAATTGAAAACTTAA+TGG + Chr4:91024290-91024309 None:intergenic 20.0%
!! ACACATCAATGTTTAATTAA+AGG + Chr4:91025084-91025103 None:intergenic 20.0%
!! AGAATTAAATCTTACTATGA+GGG - Chr4:91024565-91024584 MsG0480023898.01.T01:intron 20.0%
!! ATATAAATAAAGGATTAGAG+AGG - Chr4:91025182-91025201 MsG0480023898.01.T01:intron 20.0%
!! ATTCAAATGGATGATAAATT+GGG + Chr4:91025784-91025803 None:intergenic 20.0%
!! GCAAATTAAATGAACTATAT+AGG + Chr4:91025948-91025967 None:intergenic 20.0%
!! GGTTGTTAAGATATAAATAA+AGG - Chr4:91025172-91025191 MsG0480023898.01.T01:intron 20.0%
!! TATAAATAAAGGATTAGAGA+GGG - Chr4:91025183-91025202 MsG0480023898.01.T01:intron 20.0%
!! TATGTATCAAACAATAACAA+TGG - Chr4:91026320-91026339 MsG0480023898.01.T01:intron 20.0%
!! TTGGTCAAATTAATATGAAT+AGG - Chr4:91024923-91024942 MsG0480023898.01.T01:intron 20.0%
!!! TATGATCAAAACAACTATTT+TGG - Chr4:91026275-91026294 MsG0480023898.01.T01:intron 20.0%
!!! TGTAAACATTTTCATATTGA+AGG + Chr4:91024150-91024169 None:intergenic 20.0%
! AAAATATAGCGAGTATATCT+TGG - Chr4:91026580-91026599 MsG0480023898.01.T01:five_prime_UTR 25.0%
! AACAATAACAATGGACAAAT+AGG - Chr4:91026329-91026348 MsG0480023898.01.T01:intron 25.0%
! AAGATATAACAAACCAACTA+TGG + Chr4:91025324-91025343 None:intergenic 25.0%
! AATATTGTGAAAGAGAGAAA+TGG - Chr4:91024521-91024540 MsG0480023898.01.T01:intron 25.0%
! ACAAAAAATTGACCTCAAAA+GGG - Chr4:91025481-91025500 MsG0480023898.01.T01:CDS 25.0%
! ACACAATTATAGGACTAAAA+AGG + Chr4:91025244-91025263 None:intergenic 25.0%
! ACTACAATTTCTATCTTGAT+TGG + Chr4:91024437-91024456 None:intergenic 25.0%
! ATAGAAATTGTAGTTCTTGA+TGG - Chr4:91024443-91024462 MsG0480023898.01.T01:CDS 25.0%
! ATAGTAAAAAGGAATCATCA+TGG - Chr4:91024477-91024496 MsG0480023898.01.T01:CDS 25.0%
! ATATTGTGAAAGAGAGAAAT+GGG - Chr4:91024522-91024541 MsG0480023898.01.T01:intron 25.0%
! ATCTTTAACAACAAAAGCTT+CGG + Chr4:91024720-91024739 None:intergenic 25.0%
! ATTCTTTCTGACACAATTAT+AGG + Chr4:91025254-91025273 None:intergenic 25.0%
! CATTCAAATGGATGATAAAT+TGG + Chr4:91025785-91025804 None:intergenic 25.0%
! CTACAATTTCTATCTTGATT+GGG + Chr4:91024436-91024455 None:intergenic 25.0%
! GAGAATTAAATCTTACTATG+AGG - Chr4:91024564-91024583 MsG0480023898.01.T01:intron 25.0%
! GCTGTTAATTAACAATTAGT+TGG - Chr4:91025115-91025134 MsG0480023898.01.T01:intron 25.0%
! GTTATGAATGTGTCAATAAA+TGG - Chr4:91025612-91025631 MsG0480023898.01.T01:CDS 25.0%
! TTCAAATGGATGATAAATTG+GGG + Chr4:91025783-91025802 None:intergenic 25.0%
! TTTCAAAGCAATAATCATCA+AGG - Chr4:91026239-91026258 MsG0480023898.01.T01:five_prime_UTR 25.0%
!! AGATTTTCCTCATAGTAAAA+AGG - Chr4:91024466-91024485 MsG0480023898.01.T01:CDS 25.0%
!!! AACCATTTTGGAAAGAATTT+AGG + Chr4:91023619-91023638 None:intergenic 25.0%
!!! ATAGTTGGTTTGTTATATCT+TGG - Chr4:91025323-91025342 MsG0480023898.01.T01:intron 25.0%
!!! GAATTATGTTATTTCCACAT+TGG - Chr4:91025270-91025289 MsG0480023898.01.T01:intron 25.0%
!!! TTTATTTCTGTGTTCTTTCT+TGG - Chr4:91024962-91024981 MsG0480023898.01.T01:intron 25.0%
!!! TTTTAGTTAAGCCCATTTTA+AGG - Chr4:91026620-91026639 MsG0480023898.01.T01:five_prime_UTR 25.0%
AATGTGATTCTTGTTGACAA+AGG - Chr4:91024377-91024396 MsG0480023898.01.T01:CDS 30.0%
AGAGTAATTATGTACAGTAG+AGG + Chr4:91023893-91023912 None:intergenic 30.0%
AGTTAACTAATCTAGTTACC+TGG - Chr4:91025036-91025055 MsG0480023898.01.T01:intron 30.0%
ATAACAAACCAACTATGGAA+AGG + Chr4:91025319-91025338 None:intergenic 30.0%
ATATGATTAGGTGAATGAAG+AGG - Chr4:91024185-91024204 MsG0480023898.01.T01:CDS 30.0%
ATTTGACGCAACCTTAAAAT+GGG + Chr4:91026634-91026653 None:intergenic 30.0%
CAAAACACATGAAAAAGTAG+CGG - Chr4:91026528-91026547 MsG0480023898.01.T01:five_prime_UTR 30.0%
CACAAAAAATTGACCTCAAA+AGG - Chr4:91025480-91025499 MsG0480023898.01.T01:CDS 30.0%
CATGCTTGCATATAAATTCA+AGG + Chr4:91024837-91024856 None:intergenic 30.0%
CTAGAGAAAATTGGTAAAGA+TGG - Chr4:91025450-91025469 MsG0480023898.01.T01:intron 30.0%
GGTAATAGGAGAAATTGTTA+TGG - Chr4:91023928-91023947 MsG0480023898.01.T01:CDS 30.0%
GTCCTAAATTCTTTCCAAAA+TGG - Chr4:91023614-91023633 MsG0480023898.01.T01:three_prime_UTR 30.0%
GTGATGCATACATATGAAAA+GGG + Chr4:91025011-91025030 None:intergenic 30.0%
TAACAAACCAACTATGGAAA+GGG + Chr4:91025318-91025337 None:intergenic 30.0%
TAGAAAAAGGTAGAAGTAGA+AGG + Chr4:91025910-91025929 None:intergenic 30.0%
TATTTGACGCAACCTTAAAA+TGG + Chr4:91026635-91026654 None:intergenic 30.0%
TGTGAATCTAAGAAGCTATA+AGG + Chr4:91026407-91026426 None:intergenic 30.0%
TGTGATGCATACATATGAAA+AGG + Chr4:91025012-91025031 None:intergenic 30.0%
TGTTATTGTTGGTGTAAGAA+AGG + Chr4:91023581-91023600 None:intergenic 30.0%
TTAAATCTTACTATGAGGGA+TGG - Chr4:91024569-91024588 MsG0480023898.01.T01:intron 30.0%
TTCTTAGATTCACAATCAGA+CGG - Chr4:91026413-91026432 MsG0480023898.01.T01:intron 30.0%
TTTAATTCTCCTGTAAGCAA+TGG + Chr4:91024554-91024573 None:intergenic 30.0%
TTTGAATGCAATATGCCAAA+TGG - Chr4:91025797-91025816 MsG0480023898.01.T01:CDS 30.0%
TTTGGCATATTGCATTCAAA+TGG + Chr4:91025797-91025816 None:intergenic 30.0%
! AAAACGTTTTGCAACCATTT+TGG + Chr4:91023631-91023650 None:intergenic 30.0%
! GATGATTCCTTTTTACTATG+AGG + Chr4:91024476-91024495 None:intergenic 30.0%
! TGAATATTGGGTTGTTTGAT+TGG + Chr4:91023749-91023768 None:intergenic 30.0%
! TGGTATTGCAATCAGATTTT+AGG - Chr4:91025669-91025688 MsG0480023898.01.T01:CDS 30.0%
! TTTAAGGTTGCGTCAAATAT+GGG - Chr4:91026636-91026655 MsG0480023898.01.T01:five_prime_UTR 30.0%
! TTTTATGAATCTCACTCTAC+AGG - Chr4:91026200-91026219 MsG0480023898.01.T01:intron 30.0%
!! ATTGCTTTGAAATTGATCCA+TGG + Chr4:91026231-91026250 None:intergenic 30.0%
!! GGTTTGTTATATCTTGGTTA+AGG - Chr4:91025329-91025348 MsG0480023898.01.T01:intron 30.0%
!! TTTGTTGTTAAAGATCATCG+CGG - Chr4:91024725-91024744 MsG0480023898.01.T01:intron 30.0%
!!! CTTTTGAGGTCAATTTTTTG+TGG + Chr4:91025482-91025501 None:intergenic 30.0%
!!! TTTTAAGGTTGCGTCAAATA+TGG - Chr4:91026635-91026654 MsG0480023898.01.T01:five_prime_UTR 30.0%
AATTCATGTGACAGGGTAAT+AGG - Chr4:91023914-91023933 MsG0480023898.01.T01:CDS 35.0%
ACATGCAAAGACTTGTGTAA+TGG - Chr4:91025731-91025750 MsG0480023898.01.T01:CDS 35.0%
ACGTATCCAGCTAGAATTAT+CGG + Chr4:91024624-91024643 None:intergenic 35.0%
AGTAGAATACTAGACATGGA+TGG - Chr4:91024344-91024363 MsG0480023898.01.T01:CDS 35.0%
ATATGCTAGATGGAATGAGT+TGG - Chr4:91025999-91026018 MsG0480023898.01.T01:five_prime_UTR 35.0%
ATGAATAGTGATGTGGGAAA+TGG + Chr4:91026371-91026390 None:intergenic 35.0%
ATGGTTAGAGCTAACATCAA+TGG - Chr4:91025816-91025835 MsG0480023898.01.T01:CDS 35.0%
ATTCTACTACCGGTGAATAT+TGG + Chr4:91024332-91024351 None:intergenic 35.0%
ATTTCTATCTTGATTGGGTG+AGG + Chr4:91024431-91024450 None:intergenic 35.0%
CAATCATTGATAGAGCTTCA+AGG - Chr4:91026465-91026484 MsG0480023898.01.T01:intron 35.0%
CATGCAAAGACTTGTGTAAT+GGG - Chr4:91025732-91025751 MsG0480023898.01.T01:CDS 35.0%
CATGTCTAGTATTCTACTAC+CGG + Chr4:91024342-91024361 None:intergenic 35.0%
CCACATCACTATTCATGAAT+GGG - Chr4:91026375-91026394 MsG0480023898.01.T01:intron 35.0%
CCATTCATGAATAGTGATGT+GGG + Chr4:91026377-91026396 None:intergenic 35.0%
CGAGACATTAACAACACATT+GGG - Chr4:91025855-91025874 MsG0480023898.01.T01:CDS 35.0%
CTTACCACTCTTCTAAGTAA+AGG + Chr4:91023989-91024008 None:intergenic 35.0%
CTTGAAGCTCTATCAATGAT+TGG + Chr4:91026467-91026486 None:intergenic 35.0%
CTTTCACTTCCAATATTCAC+CGG - Chr4:91024320-91024339 MsG0480023898.01.T01:intron 35.0%
GGGTGAAAATAAAATGCCTA+TGG + Chr4:91025298-91025317 None:intergenic 35.0%
TACTCTCAATTCATGTGACA+GGG - Chr4:91023907-91023926 MsG0480023898.01.T01:CDS 35.0%
TATTTCCACATTGGTACCAT+AGG - Chr4:91025279-91025298 MsG0480023898.01.T01:intron 35.0%
TGGAATGTCAGAGAAAATGT+GGG - Chr4:91025695-91025714 MsG0480023898.01.T01:CDS 35.0%
TTACTCTCAATTCATGTGAC+AGG - Chr4:91023906-91023925 MsG0480023898.01.T01:CDS 35.0%
TTGGAATGTCAGAGAAAATG+TGG - Chr4:91025694-91025713 MsG0480023898.01.T01:CDS 35.0%
! AACATGCAAAACCTTTTCTC+TGG - Chr4:91023956-91023975 MsG0480023898.01.T01:CDS 35.0%
! AACGTTGCTTTGTTAACTCA+AGG - Chr4:91026048-91026067 MsG0480023898.01.T01:intron 35.0%
! ATTTTCACCCTTTCCATAGT+TGG - Chr4:91025308-91025327 MsG0480023898.01.T01:intron 35.0%
! CCAATGTGTTGTTAATGTCT+CGG + Chr4:91025857-91025876 None:intergenic 35.0%
! TGCAATCAGATTTTAGGCAT+TGG - Chr4:91025675-91025694 MsG0480023898.01.T01:CDS 35.0%
!! GGGTTGTTTGATTGGTTTAA+GGG + Chr4:91023741-91023760 None:intergenic 35.0%
!! TGATGTTAGCTCTAACCATT+TGG + Chr4:91025815-91025834 None:intergenic 35.0%
!! TGGGTTGTTTGATTGGTTTA+AGG + Chr4:91023742-91023761 None:intergenic 35.0%
AAATGCCTATGGTACCAATG+TGG + Chr4:91025287-91025306 None:intergenic 40.0%
AACAAACGTTATGTCTACCG+TGG - Chr4:91025756-91025775 MsG0480023898.01.T01:CDS 40.0%
AATAGTGATGTGGGAAATGG+TGG + Chr4:91026368-91026387 None:intergenic 40.0%
ACAATAACATCAGAGCAAGC+TGG - Chr4:91023592-91023611 MsG0480023898.01.T01:three_prime_UTR 40.0%
ACACATGAAAAAGTAGCGGT+TGG - Chr4:91026532-91026551 MsG0480023898.01.T01:five_prime_UTR 40.0%
AGTTCACCGATAATTCTAGC+TGG - Chr4:91024615-91024634 MsG0480023898.01.T01:intron 40.0%
CATTAACAACACATTGGGCA+AGG - Chr4:91025860-91025879 MsG0480023898.01.T01:CDS 40.0%
CATTGCTTGAAGTCTCATGA+AGG + Chr4:91024261-91024280 None:intergenic 40.0%
CCCACATCACTATTCATGAA+TGG - Chr4:91026374-91026393 MsG0480023898.01.T01:intron 40.0%
CCCATTCATGAATAGTGATG+TGG + Chr4:91026378-91026397 None:intergenic 40.0%
CCGAGACATTAACAACACAT+TGG - Chr4:91025854-91025873 MsG0480023898.01.T01:CDS 40.0%
CGGTAGTAGAATACTAGACA+TGG - Chr4:91024340-91024359 MsG0480023898.01.T01:CDS 40.0%
CTTCATGAGACTTCAAGCAA+TGG - Chr4:91024259-91024278 MsG0480023898.01.T01:intron 40.0%
GCTTGCTCTGATGTTATTGT+TGG + Chr4:91023592-91023611 None:intergenic 40.0%
GGAACCTTTACTTAGAAGAG+TGG - Chr4:91023982-91024001 MsG0480023898.01.T01:intron 40.0%
GTAGACTTGTAGGGACTATT+TGG + Chr4:91024409-91024428 None:intergenic 40.0%
GTGAACTCAATATCCCCAAT+TGG + Chr4:91024602-91024621 None:intergenic 40.0%
TATGAATCTCACTCTACAGG+TGG - Chr4:91026203-91026222 MsG0480023898.01.T01:intron 40.0%
TCATTGATAGAGCTTCAAGG+TGG - Chr4:91026468-91026487 MsG0480023898.01.T01:intron 40.0%
TGATTAGGTGAATGAAGAGG+TGG - Chr4:91024188-91024207 MsG0480023898.01.T01:CDS 40.0%
TTAGATTCACAATCAGACGG+TGG - Chr4:91026416-91026435 MsG0480023898.01.T01:intron 40.0%
! ATGATTGGTCCTGAACTTGA+AGG + Chr4:91026452-91026471 None:intergenic 40.0%
! GTGTTGTTAATGTCTCGGAT+GGG + Chr4:91025852-91025871 None:intergenic 40.0%
! TGAGAGAAGCATATGCTAGA+TGG - Chr4:91025989-91026008 MsG0480023898.01.T01:five_prime_UTR 40.0%
! TGTGTTGTTAATGTCTCGGA+TGG + Chr4:91025853-91025872 None:intergenic 40.0%
! TTAAAGGTGCAATTACCACC+AGG + Chr4:91025068-91025087 None:intergenic 40.0%
! TTGCTGTTAGAATTGCTCCT+GGG - Chr4:91024660-91024679 MsG0480023898.01.T01:intron 40.0%
!! AAAGAAAAGCATCCAGTTCC+AGG - Chr4:91025213-91025232 MsG0480023898.01.T01:intron 40.0%
!! AAGAAAAGCATCCAGTTCCA+GGG - Chr4:91025214-91025233 MsG0480023898.01.T01:intron 40.0%
!! GACTTTGTTATTGATCGTGG+TGG + Chr4:91024237-91024256 None:intergenic 40.0%
!! GGTGACTTTGTTATTGATCG+TGG + Chr4:91024240-91024259 None:intergenic 40.0%
AAAAAGGTTGACCCTGGAAC+TGG + Chr4:91025228-91025247 None:intergenic 45.0%
AAGGTTCCAAGCCAGAGAAA+AGG + Chr4:91023970-91023989 None:intergenic 45.0%
AGGACTAAAAAGGTTGACCC+TGG + Chr4:91025234-91025253 None:intergenic 45.0%
AGGGATGGAATTGCACCAAT+TGG - Chr4:91024584-91024603 MsG0480023898.01.T01:intron 45.0%
ATTACCACCAGGTACATACC+AGG + Chr4:91025057-91025076 None:intergenic 45.0%
ATTGGGTGAGGTAGACTTGT+AGG + Chr4:91024419-91024438 None:intergenic 45.0%
CACACTTCGTCCTTAGATTG+GGG + Chr4:91025426-91025445 None:intergenic 45.0%
CCACACTTCGTCCTTAGATT+GGG + Chr4:91025427-91025446 None:intergenic 45.0%
CTAGTTACCTGGTATGTACC+TGG - Chr4:91025047-91025066 MsG0480023898.01.T01:intron 45.0%
CTTGAGAAACTCTTGCACTG+TGG + Chr4:91025511-91025530 None:intergenic 45.0%
GACAAATAGGTTGCATCGAG+GGG - Chr4:91026342-91026361 MsG0480023898.01.T01:intron 45.0%
GAGCCTATGAGGATCAACTA+CGG + Chr4:91025538-91025557 None:intergenic 45.0%
GGACAAATAGGTTGCATCGA+GGG - Chr4:91026341-91026360 MsG0480023898.01.T01:intron 45.0%
GGATGATAAATTGGGGTCCA+CGG + Chr4:91025776-91025795 None:intergenic 45.0%
GGATGGAATTGCACCAATTG+GGG - Chr4:91024586-91024605 MsG0480023898.01.T01:intron 45.0%
GGGAAAAGACCATTGCTTAC+AGG - Chr4:91024542-91024561 MsG0480023898.01.T01:intron 45.0%
GGGATGGAATTGCACCAATT+GGG - Chr4:91024585-91024604 MsG0480023898.01.T01:intron 45.0%
GTGTAAGAAAGGTTGCAGTG+AGG + Chr4:91023570-91023589 None:intergenic 45.0%
GTGTGGCGTCTAGAGAAAAT+TGG - Chr4:91025441-91025460 MsG0480023898.01.T01:intron 45.0%
GTTAATGTCTCGGATGGGAA+TGG + Chr4:91025847-91025866 None:intergenic 45.0%
GTTCTCATGCGTTTGTCGTT+TGG + Chr4:91023683-91023702 None:intergenic 45.0%
TGATCCTCATAGGCTCAGAA+AGG - Chr4:91025542-91025561 MsG0480023898.01.T01:CDS 45.0%
TGGACAAATAGGTTGCATCG+AGG - Chr4:91026340-91026359 MsG0480023898.01.T01:intron 45.0%
TGTACCTTTCTGAGCCTATG+AGG + Chr4:91025549-91025568 None:intergenic 45.0%
TGTCCGTAGTTGATCCTCAT+AGG - Chr4:91025532-91025551 MsG0480023898.01.T01:CDS 45.0%
TTGGGTGAGGTAGACTTGTA+GGG + Chr4:91024418-91024437 None:intergenic 45.0%
! CAAAGTGTCCGAATTCGTGA+AGG - Chr4:91024689-91024708 MsG0480023898.01.T01:intron 45.0%
! CACTGTGGTAATCCCTTTTG+AGG + Chr4:91025496-91025515 None:intergenic 45.0%
! GATCTAACGCCTTCAAGTTC+AGG - Chr4:91026440-91026459 MsG0480023898.01.T01:intron 45.0%
! GCAAAACCTTTTCTCTGGCT+TGG - Chr4:91023961-91023980 MsG0480023898.01.T01:CDS 45.0%
! GTTGCTGTTAGAATTGCTCC+TGG - Chr4:91024659-91024678 MsG0480023898.01.T01:intron 45.0%
! TGCTAGATGGAATGAGTTGG+AGG - Chr4:91026002-91026021 MsG0480023898.01.T01:intron 45.0%
! TTTGGTGATACTCTCGAGAC+TGG - Chr4:91026293-91026312 MsG0480023898.01.T01:intron 45.0%
!! GGAATCATCATGGACTAGTG+AGG - Chr4:91024487-91024506 MsG0480023898.01.T01:CDS 45.0%
AAACCACCCACCCCAATCTA+AGG - Chr4:91025413-91025432 MsG0480023898.01.T01:intron 50.0%
ACTTCGTCCTTAGATTGGGG+TGG + Chr4:91025423-91025442 None:intergenic 50.0%
CCCAATCTAAGGACGAAGTG+TGG - Chr4:91025424-91025443 MsG0480023898.01.T01:intron 50.0%
CGGTCATGCCTTCACGAATT+CGG + Chr4:91024700-91024719 None:intergenic 50.0%
CTTCGTCCTTAGATTGGGGT+GGG + Chr4:91025422-91025441 None:intergenic 50.0%
GCCACACTTCGTCCTTAGAT+TGG + Chr4:91025428-91025447 None:intergenic 50.0%
GTTACCTGGTATGTACCTGG+TGG - Chr4:91025050-91025069 MsG0480023898.01.T01:intron 50.0%
TCACTCTACAGGTGGAACCA+TGG - Chr4:91026211-91026230 MsG0480023898.01.T01:intron 50.0%
TGTCTCGGATGGGAATGGTT+TGG + Chr4:91025842-91025861 None:intergenic 50.0%
! CGGACACTTTGATTAGTCCC+AGG + Chr4:91024680-91024699 None:intergenic 50.0%
CGTCCTTAGATTGGGGTGGG+TGG + Chr4:91025419-91025438 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 91023544 91026671 91023544 ID=MsG0480023898.01;Name=MsG0480023898.01
Chr4 mRNA 91023544 91026671 91023544 ID=MsG0480023898.01.T01;Parent=MsG0480023898.01;Name=MsG0480023898.01.T01;_AED=0.26;_eAED=0.26;_QI=291|0.8|0.83|1|0.6|0.5|6|175|363
Chr4 exon 91023544 91023993 91023544 ID=MsG0480023898.01.T01:exon:33924;Parent=MsG0480023898.01.T01
Chr4 exon 91024146 91024245 91024146 ID=MsG0480023898.01.T01:exon:33923;Parent=MsG0480023898.01.T01
Chr4 exon 91024334 91024524 91024334 ID=MsG0480023898.01.T01:exon:33922;Parent=MsG0480023898.01.T01
Chr4 exon 91025463 91026020 91025463 ID=MsG0480023898.01.T01:exon:33921;Parent=MsG0480023898.01.T01
Chr4 exon 91026212 91026287 91026212 ID=MsG0480023898.01.T01:exon:33920;Parent=MsG0480023898.01.T01
Chr4 exon 91026489 91026671 91026489 ID=MsG0480023898.01.T01:exon:33919;Parent=MsG0480023898.01.T01
Chr4 five_prime_UTR 91026489 91026671 91026489 ID=MsG0480023898.01.T01:five_prime_utr;Parent=MsG0480023898.01.T01
Chr4 five_prime_UTR 91026212 91026287 91026212 ID=MsG0480023898.01.T01:five_prime_utr;Parent=MsG0480023898.01.T01
Chr4 five_prime_UTR 91025989 91026020 91025989 ID=MsG0480023898.01.T01:five_prime_utr;Parent=MsG0480023898.01.T01
Chr4 CDS 91025463 91025988 91025463 ID=MsG0480023898.01.T01:cds;Parent=MsG0480023898.01.T01
Chr4 CDS 91024334 91024524 91024334 ID=MsG0480023898.01.T01:cds;Parent=MsG0480023898.01.T01
Chr4 CDS 91024146 91024245 91024146 ID=MsG0480023898.01.T01:cds;Parent=MsG0480023898.01.T01
Chr4 CDS 91023719 91023993 91023719 ID=MsG0480023898.01.T01:cds;Parent=MsG0480023898.01.T01
Chr4 three_prime_UTR 91023544 91023718 91023544 ID=MsG0480023898.01.T01:three_prime_utr;Parent=MsG0480023898.01.T01
Gene Sequence

>MsG0480023898.01.T01

ATGTTATCCACCACGATCAATAACAAAGTCACCTTCATGAGACTTCAAGCAATGGAAGAACCATTAAGTTTTCAATTCATGTTCAAAAATAAACTTTCACTTCCAATATTCACCGGTAGTAGAATACTAGACATGGATGGAAACTCAATCAATGTGATTCTTGTTGACAAAGGCAATGACCAAATAGTCCCTACAAGTCTACCTCACCCAATCAAGATAGAAATTGTAGTTCTTGATGGAGATTTTCCTCATAGTAAAAAGGAATCATCATGGACTAGTGAGGAATTCAATAGCAATATTGTGAAAGAGAGAAATGGGAAAAGACCATTGCTTACAGGAGAATTAAATCTTACTATGAGGGATGGAATTGCACCAATTGGGGATATTGAGTTCACCGATAATTCTAGCTGGATACGTAGTAGAAAGTTTAGAGTTGCTGTTAGAATTGCTCCTGGGACTAATCAAAGTGTCCGAATTCGTGAAGGCATGACCGAAGCTTTTGTTGTTAAAGATCATCGCGGAGAAAGCATTGGAATGTCAGAGAAAATGTGGGATGTGACAATTAAACATGCAAAGACTTGTGTAATGGGAAACAAACGTTATGTCTACCGTGGACCCCAATTTATCATCCATTTGAATGCAATATGCCAAATGGTTAGAGCTAACATCAATGGCCAAACCATTCCCATCCGAGACATTAACAACACATTGGGCAAGAGCTACATACAAAACTATGTGAGAGAAGCATATGCTAGATGGAATGAGTTGGAGGAAATTGATGAAGTTTTGAATGACAACGTTGCTTTGTTAACTCAAGGTGGAACCATGGATCAATTTCAAAGCAATAATCATCAAGGATCACTTATAACATATGATCAAAACAACTATTTTGGTGATACTCTCGAGACTGGCAACTATGTATCAAACAATAACAATGGACAAATAGGTTGCATCGAGGGGCCACCATTTCCCACATCACTATTCATGAATGGGATCATACCTTATAGCTTCTTAGATTCACAATCAGACGGTGGTGATCTAACGCCTTCAAGTTCAGGACCAATCATTGATAGAGCTTCAAGGTGGCATTAG
Protein sequence

>MsG0480023898.01.T01

MLSTTINNKVTFMRLQAMEEPLSFQFMFKNKLSLPIFTGSRILDMDGNSINVILVDKGNDQIVPTSLPHPIKIEIVVLDGDFPHSKKESSWTSEEFNSNIVKERNGKRPLLTGELNLTMRDGIAPIGDIEFTDNSSWIRSRKFRVAVRIAPGTNQSVRIREGMTEAFVVKDHRGESIGMSEKMWDVTIKHAKTCVMGNKRYVYRGPQFIIHLNAICQMVRANINGQTIPIRDINNTLGKSYIQNYVREAYARWNELEEIDEVLNDNVALLTQGGTMDQFQSNNHQGSLITYDQNNYFGDTLETGNYVSNNNNGQIGCIEGPPFPTSLFMNGIIPYSFLDSQSDGGDLTPSSSGPIIDRASRWH*