Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004766.01.T01 | AES61601.1 | 83.621 | 116 | 17 | 2 | 1 | 114 | 1 | 116 | 1.21E-40 | 140 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004766.01.T01 | G7ICT0 | 83.621 | 116 | 17 | 2 | 1 | 114 | 1 | 116 | 5.76e-41 | 140 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000525.01 | MsG0180004766.01 | 0.861290 | 1.138596e-63 | 3.205836e-60 |
| MsG0180000526.01 | MsG0180004766.01 | 0.801274 | 9.728307e-49 | 4.626761e-46 |
| MsG0180004765.01 | MsG0180004766.01 | 0.814866 | 1.238813e-51 | 8.408390e-49 |
| MsG0180004766.01 | MsG0480023383.01 | 0.807375 | 5.212599e-50 | 2.898574e-47 |
| MsG0180004766.01 | MsG0680035580.01 | 0.813947 | 1.977592e-51 | 1.309356e-48 |
| MsG0180004766.01 | MsG0780039432.01 | 0.834290 | 3.264480e-56 | 3.857101e-53 |
| MsG0180004766.01 | MsG0780039433.01 | 0.859985 | 2.830345e-63 | 7.617908e-60 |
| MsG0180004766.01 | MsG0780041475.01 | 0.829797 | 4.210092e-55 | 4.355033e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004766.01.T01 | MTR_1g086450 | 83.621 | 116 | 17 | 2 | 1 | 114 | 1 | 116 | 1.46e-44 | 140 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGAGGAGGAAGAAGAAGATA+TGG | 0.309577 | 1:+82459065 | MsG0180004766.01.T01:CDS |
| CACGTAGTTCTAATTCTCTT+TGG | 0.313385 | 1:-82459146 | None:intergenic |
| CTTCCTCCTCGTTCTTATTG+AGG | 0.398320 | 1:-82459053 | None:intergenic |
| CTCTTTGGCGTAGTCGATCA+CGG | 0.421114 | 1:-82459131 | None:intergenic |
| ACGATTAAAGAGCAAGTTTG+AGG | 0.460676 | 1:-82459347 | None:intergenic |
| AAAGAGCAAGTTTGAGGTCA+AGG | 0.490588 | 1:-82459341 | None:intergenic |
| ATTAGAACTACGTGATCAGC+TGG | 0.493231 | 1:+82459155 | MsG0180004766.01.T01:CDS |
| AAATAACAAAGTTGAGAAGA+AGG | 0.516605 | 1:+82459311 | MsG0180004766.01.T01:CDS |
| CTGGGTTGATAAAATTCAAA+GGG | 0.533763 | 1:-82459266 | None:intergenic |
| TCTTTGGCGTAGTCGATCAC+GGG | 0.543652 | 1:-82459130 | None:intergenic |
| ACTGGGTTGATAAAATTCAA+AGG | 0.551121 | 1:-82459267 | None:intergenic |
| AAGAGCAAGTTTGAGGTCAA+GGG | 0.558615 | 1:-82459340 | None:intergenic |
| AAGTCCTGCAATTCAAAACA+AGG | 0.614434 | 1:-82459216 | None:intergenic |
| GTAGTCGATCACGGGCGTCA+CGG | 0.615898 | 1:-82459122 | None:intergenic |
| TGTCCTCAATAAGAACGAGG+AGG | 0.708771 | 1:+82459050 | MsG0180004766.01.T01:CDS |
| TAATGTCCTCAATAAGAACG+AGG | 0.718289 | 1:+82459047 | MsG0180004766.01.T01:CDS |
| TATGGAAAACAAGAAGAACG+AGG | 0.749778 | 1:+82459020 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATAACAAAGTTGAGAAGA+AGG | + | Chr1:82459311-82459330 | MsG0180004766.01.T01:CDS | 25.0% |
| !! | ACTGGGTTGATAAAATTCAA+AGG | - | Chr1:82459270-82459289 | None:intergenic | 30.0% |
| !! | ATGATTTTGTCATTTGCAAC+TGG | - | Chr1:82459288-82459307 | None:intergenic | 30.0% |
| !! | CTGGGTTGATAAAATTCAAA+GGG | - | Chr1:82459269-82459288 | None:intergenic | 30.0% |
| !! | TGATTTTGTCATTTGCAACT+GGG | - | Chr1:82459287-82459306 | None:intergenic | 30.0% |
| AAGTCCTGCAATTCAAAACA+AGG | - | Chr1:82459219-82459238 | None:intergenic | 35.0% | |
| TAATGTCCTCAATAAGAACG+AGG | + | Chr1:82459047-82459066 | MsG0180004766.01.T01:CDS | 35.0% | |
| !! | CACGTAGTTCTAATTCTCTT+TGG | - | Chr1:82459149-82459168 | None:intergenic | 35.0% |
| ATTAGAACTACGTGATCAGC+TGG | + | Chr1:82459155-82459174 | MsG0180004766.01.T01:CDS | 40.0% | |
| ! | AAAGAGCAAGTTTGAGGTCA+AGG | - | Chr1:82459344-82459363 | None:intergenic | 40.0% |
| ! | AAGAGCAAGTTTGAGGTCAA+GGG | - | Chr1:82459343-82459362 | None:intergenic | 40.0% |
| !!! | AGAGCCTTGTTTTGAATTGC+AGG | + | Chr1:82459212-82459231 | MsG0180004766.01.T01:CDS | 40.0% |
| CGAGGAGGAAGAAGAAGATA+TGG | + | Chr1:82459065-82459084 | MsG0180004766.01.T01:CDS | 45.0% | |
| CTTCCTCCTCGTTCTTATTG+AGG | - | Chr1:82459056-82459075 | None:intergenic | 45.0% | |
| TGTCCTCAATAAGAACGAGG+AGG | + | Chr1:82459050-82459069 | MsG0180004766.01.T01:CDS | 45.0% | |
| CTCTTTGGCGTAGTCGATCA+CGG | - | Chr1:82459134-82459153 | None:intergenic | 50.0% | |
| TCTTTGGCGTAGTCGATCAC+GGG | - | Chr1:82459133-82459152 | None:intergenic | 50.0% | |
| GTAGTCGATCACGGGCGTCA+CGG | - | Chr1:82459125-82459144 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 82459021 | 82459365 | 82459021 | ID=MsG0180004766.01;Name=MsG0180004766.01 |
| Chr1 | mRNA | 82459021 | 82459365 | 82459021 | ID=MsG0180004766.01.T01;Parent=MsG0180004766.01;Name=MsG0180004766.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|114 |
| Chr1 | exon | 82459021 | 82459365 | 82459021 | ID=MsG0180004766.01.T01:exon:27278;Parent=MsG0180004766.01.T01 |
| Chr1 | CDS | 82459021 | 82459365 | 82459021 | ID=MsG0180004766.01.T01:cds;Parent=MsG0180004766.01.T01 |
| Gene Sequence |
| Protein sequence |
