AlfalfaGEDB | MsG0480023529.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023529.01

KEGG: -
GO: GO:0055114
PFAM: PF08240

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023529.01.T01	AET04881.1	93.893	131	5	2	1	131	1	128	5.45E-81	251

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023529.01.T01	A4YGN0	49.275	69	35	0	57	125	1	69	2.77E-14	71.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023529.01.T01	G7LFT7	93.893	131	5	2	1	131	1	128	2.60e-81	251

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180005272.01	MsG0480023529.01	0.820607	6.273799e-53	4.987028e-50
MsG0280011165.01	MsG0480023529.01	0.803925	2.762830e-49	1.405954e-46
MsG0280011203.01	MsG0480023529.01	0.800582	1.347349e-48	6.296609e-46
MsG0480022953.01	MsG0480023529.01	0.820254	7.558480e-53	5.950235e-50
MsG0480023267.01	MsG0480023529.01	0.801574	8.442823e-49	4.045874e-46
MsG0480023528.01	MsG0480023529.01	0.889376	2.561286e-73	2.061216e-69
MsG0480023529.01	MsG0780039969.01	0.835650	1.483054e-56	1.825057e-53
MsG0480023529.01	MsG0780040173.01	0.833881	4.132499e-56	4.823056e-53

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023529.01.T01	MTR_8g095220	93.893	131	5	2	1	131	1	128	6.60e-85	251
MsG0480023529.01.T01	MTR_8g095220	93.893	131	5	2	1	131	1	128	2.80e-84	253
MsG0480023529.01.T01	MTR_7g099040	41.558	77	43	2	58	132	13	89	8.97e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023529.01.T01	AT5G63620	58.209	134	47	3	1	131	3	130	2.88e-45	150
MsG0480023529.01.T01	AT5G63620	58.209	134	47	3	1	131	3	130	9.55e-45	151
MsG0480023529.01.T01	AT5G63620	58.209	134	47	3	1	131	3	130	1.11e-44	150

Find 32 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTTTAACCTTTCTATGGTT+TGG	0.309846	4:+86485958	None:intergenic
GATGAGAAGTTCACCAGCTT+TGG	0.318763	4:+86486323	None:intergenic
TTCAATGGTGAGAGGTTTGT+TGG	0.348495	4:+86486365	None:intergenic
ATGAGAAGTTCACCAGCTTT+GGG	0.377738	4:+86486324	None:intergenic
TCTTGAAAAGGTTGTCTTGT+TGG	0.386179	4:-86486515	MsG0480023529.01.T01:CDS
GTTGTGGGCCATGAGATTAC+TGG	0.411881	4:-86486013	MsG0480023529.01.T01:CDS
TTGTTTCATTTCAGTTTCAT+TGG	0.430102	4:-86486616	MsG0480023529.01.T01:five_prime_UTR
ACCAACAGCTTCTGTGTGAA+TGG	0.454170	4:+86486434	None:intergenic
ACAGAAGCTGTTGGTGGTGT+TGG	0.457231	4:-86486426	MsG0480023529.01.T01:CDS
TCAGCAACAACTTCTTGAAA+AGG	0.464826	4:-86486527	MsG0480023529.01.T01:CDS
TCAATGGTGAGAGGTTTGTT+GGG	0.468644	4:+86486366	None:intergenic
TTTCAGTTTCATTGGAAGAT+CGG	0.481224	4:-86486608	MsG0480023529.01.T01:five_prime_UTR
CATATTCCTCGTCCCAAAGC+TGG	0.483522	4:-86486336	MsG0480023529.01.T01:CDS
AGTTCACCAGCTTTGGGACG+AGG	0.494387	4:+86486330	None:intergenic
GTCTAAGTGAGATTGAGAAA+TGG	0.520409	4:-86486577	MsG0480023529.01.T01:exon
AGGAATATGAAATTCTTCAA+TGG	0.521033	4:+86486350	None:intergenic
ACAACACAAGGACTTGAAAA+CGG	0.537012	4:+86486031	None:intergenic
ACAGCACCACGCATGTAAGA+TGG	0.560649	4:+86486399	None:intergenic
ATCTCATGGCCCACAACACA+AGG	0.562994	4:+86486019	None:intergenic
TCTGATCTTCATGTCATGAA+AGG	0.564745	4:-86486061	MsG0480023529.01.T01:CDS
TCCATTCACACAGAAGCTGT+TGG	0.570592	4:-86486435	MsG0480023529.01.T01:CDS
TGAAATTCTTCAATGGTGAG+AGG	0.583588	4:+86486357	None:intergenic
TGTTGAAGCTGATGCATGTA+CGG	0.583923	4:-86486460	MsG0480023529.01.T01:CDS
ATTCACACAGAAGCTGTTGG+TGG	0.585825	4:-86486432	MsG0480023529.01.T01:CDS
CAACTTCACCAGTAATCTCA+TGG	0.594487	4:+86486005	None:intergenic
TGAAATGAAACAAAACAAAG+TGG	0.605297	4:+86486626	None:intergenic
TGGCATCTGCACTAGTTCGT+AGG	0.610288	4:-86486557	MsG0480023529.01.T01:CDS
ACTGGTGAAGTTGTTGAACA+TGG	0.627651	4:-86485995	MsG0480023529.01.T01:CDS
GAACTTCTCATCAAAACCAA+GGG	0.629940	4:-86486312	MsG0480023529.01.T01:intron
TGAACTTCTCATCAAAACCA+AGG	0.647879	4:-86486313	MsG0480023529.01.T01:CDS
GGATTGCCATCTTACATGCG+TGG	0.668555	4:-86486405	MsG0480023529.01.T01:CDS
TCATGACATGAAGATCAGAG+TGG	0.751085	4:+86486065	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTTGATATTGTTATGAT+TGG	-	Chr4:86486495-86486514	MsG0480023529.01.T01:CDS	15.0%
!	AGGAATATGAAATTCTTCAA+TGG	+	Chr4:86486298-86486317	None:intergenic	25.0%
!	TGAAATGAAACAAAACAAAG+TGG	+	Chr4:86486022-86486041	None:intergenic	25.0%
!	TTGTTTCATTTCAGTTTCAT+TGG	-	Chr4:86486029-86486048	MsG0480023529.01.T01:CDS	25.0%
!	TTTGATATTGTTATGATTGG+TGG	-	Chr4:86486498-86486517	MsG0480023529.01.T01:CDS	25.0%
!!	TTATGATTGGTGGTTATTAT+TGG	-	Chr4:86486508-86486527	MsG0480023529.01.T01:CDS	25.0%
	TGTATATTGCAGCTTAATTG+TGG	-	Chr4:86486447-86486466	MsG0480023529.01.T01:CDS	30.0%
	TTTCAGTTTCATTGGAAGAT+CGG	-	Chr4:86486037-86486056	MsG0480023529.01.T01:CDS	30.0%
!	AGCTGCAATATACAAGTTTT+AGG	+	Chr4:86486441-86486460	None:intergenic	30.0%
!!!	AGATGTGATTTTTTGAATGC+AGG	-	Chr4:86486545-86486564	MsG0480023529.01.T01:CDS	30.0%
	ACAACACAAGGACTTGAAAA+CGG	+	Chr4:86486617-86486636	None:intergenic	35.0%
	AGAGAGATAAACAATACCCT+TGG	+	Chr4:86486352-86486371	None:intergenic	35.0%
	GAACTTCTCATCAAAACCAA+GGG	-	Chr4:86486333-86486352	MsG0480023529.01.T01:CDS	35.0%
	GTCTAAGTGAGATTGAGAAA+TGG	-	Chr4:86486068-86486087	MsG0480023529.01.T01:CDS	35.0%
	TCAGCAACAACTTCTTGAAA+AGG	-	Chr4:86486118-86486137	MsG0480023529.01.T01:intron	35.0%
	TCTGATCTTCATGTCATGAA+AGG	-	Chr4:86486584-86486603	MsG0480023529.01.T01:five_prime_UTR	35.0%
	TCTTGAAAAGGTTGTCTTGT+TGG	-	Chr4:86486130-86486149	MsG0480023529.01.T01:intron	35.0%
	TGAAATTCTTCAATGGTGAG+AGG	+	Chr4:86486291-86486310	None:intergenic	35.0%
	TGAACTTCTCATCAAAACCA+AGG	-	Chr4:86486332-86486351	MsG0480023529.01.T01:CDS	35.0%
!!!	GATTTTTTGAATGCAGGATG+TGG	-	Chr4:86486551-86486570	MsG0480023529.01.T01:CDS	35.0%
!!!	TTTTCAAGTCCTTGTGTTGT+GGG	-	Chr4:86486617-86486636	MsG0480023529.01.T01:five_prime_UTR	35.0%
	ACTGGTGAAGTTGTTGAACA+TGG	-	Chr4:86486650-86486669	MsG0480023529.01.T01:five_prime_UTR	40.0%
	ATGAGAAGTTCACCAGCTTT+GGG	+	Chr4:86486324-86486343	None:intergenic	40.0%
	CAACTTCACCAGTAATCTCA+TGG	+	Chr4:86486643-86486662	None:intergenic	40.0%
	TCATGACATGAAGATCAGAG+TGG	+	Chr4:86486583-86486602	None:intergenic	40.0%
	TGTTGAAGCTGATGCATGTA+CGG	-	Chr4:86486185-86486204	MsG0480023529.01.T01:intron	40.0%
	TTCAATGGTGAGAGGTTTGT+TGG	+	Chr4:86486283-86486302	None:intergenic	40.0%
	TTGCAGCTTAATTGTGGTTG+TGG	-	Chr4:86486453-86486472	MsG0480023529.01.T01:CDS	40.0%
!	TCAATGGTGAGAGGTTTGTT+GGG	+	Chr4:86486282-86486301	None:intergenic	40.0%
!!!	GTTTTCAAGTCCTTGTGTTG+TGG	-	Chr4:86486616-86486635	MsG0480023529.01.T01:five_prime_UTR	40.0%
	ACCAACAGCTTCTGTGTGAA+TGG	+	Chr4:86486214-86486233	None:intergenic	45.0%
	ATTCACACAGAAGCTGTTGG+TGG	-	Chr4:86486213-86486232	MsG0480023529.01.T01:intron	45.0%
	GATGAGAAGTTCACCAGCTT+TGG	+	Chr4:86486325-86486344	None:intergenic	45.0%
	TCCATTCACACAGAAGCTGT+TGG	-	Chr4:86486210-86486229	MsG0480023529.01.T01:intron	45.0%
!!!	ACATGCGTGGTGCTGTTTTT+CGG	-	Chr4:86486253-86486272	MsG0480023529.01.T01:intron	45.0%
	ACAGCACCACGCATGTAAGA+TGG	+	Chr4:86486249-86486268	None:intergenic	50.0%
	ATCTCATGGCCCACAACACA+AGG	+	Chr4:86486629-86486648	None:intergenic	50.0%
	CATATTCCTCGTCCCAAAGC+TGG	-	Chr4:86486309-86486328	MsG0480023529.01.T01:intron	50.0%
	GGATTGCCATCTTACATGCG+TGG	-	Chr4:86486240-86486259	MsG0480023529.01.T01:intron	50.0%
	GTTGTGGGCCATGAGATTAC+TGG	-	Chr4:86486632-86486651	MsG0480023529.01.T01:five_prime_UTR	50.0%
	TGGCATCTGCACTAGTTCGT+AGG	-	Chr4:86486088-86486107	MsG0480023529.01.T01:intron	50.0%
!!	ACAGAAGCTGTTGGTGGTGT+TGG	-	Chr4:86486219-86486238	MsG0480023529.01.T01:intron	50.0%
!!!	CATGCGTGGTGCTGTTTTTC+GGG	-	Chr4:86486254-86486273	MsG0480023529.01.T01:intron	50.0%
!!	AGTTCACCAGCTTTGGGACG+AGG	+	Chr4:86486318-86486337	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	86485970	86486697	86485970	ID=MsG0480023529.01;Name=MsG0480023529.01
Chr4	mRNA	86485970	86486697	86485970	ID=MsG0480023529.01.T01;Parent=MsG0480023529.01;Name=MsG0480023529.01.T01;_AED=0.24;_eAED=0.24;_QI=117\|1\|1\|1\|0.14\|0.25\|8\|0\|132
Chr4	exon	86485970	86486100	86485970	ID=MsG0480023529.01.T01:exon:3096;Parent=MsG0480023529.01.T01
Chr4	exon	86486313	86486697	86486313	ID=MsG0480023529.01.T01:exon:3095;Parent=MsG0480023529.01.T01
Chr4	five_prime_UTR	86486581	86486697	86486581	ID=MsG0480023529.01.T01:five_prime_utr;Parent=MsG0480023529.01.T01
Chr4	CDS	86486313	86486580	86486313	ID=MsG0480023529.01.T01:cds;Parent=MsG0480023529.01.T01
Chr4	CDS	86485970	86486100	86485970	ID=MsG0480023529.01.T01:cds;Parent=MsG0480023529.01.T01

Gene Sequence

>MsG0480023529.01.T01

ATGGCATCTGCACTAGTTCGTAGGAACATCATCAGCAACAACTTCTTGAAAAGGTTGTCTTGTTGGCGTTTGAATAATAACAAACAGTTGTTGAATAATGTTGAAGCTGATGCATGTACGGTTTCCATTCACACAGAAGCTGTTGGTGGTGTTGGATTGCCATCTTACATGCGTGGTGCTGTTTTTCGGGAACCCAACAAACCTCTCACCATTGAAGAATTTCATATTCCTCGTCCCAAAGCTGGTGAACTTCTCATCAAAACCAAGGGATGTGGAGTATGCCACTCTGATCTTCATGTCATGAAAGGTGAAATTCCGTTTTCAAGTCCTTGTGTTGTGGGCCATGAGATTACTGGTGAAGTTGTTGAACATGGACAACACACTGATTCCAAACCATAG

Protein sequence

>MsG0480023529.01.T01

MASALVRRNIISNNFLKRLSCWRLNNNKQLLNNVEADACTVSIHTEAVGGVGLPSYMRGAVFREPNKPLTIEEFHIPRPKAGELLIKTKGCGVCHSDLHVMKGEIPFSSPCVVGHEITGEVVEHGQHTDSKP*