AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023528.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023528.01.T01 MTR_8g095220 43.775 249 36 4 13 198 155 362 9.16e-49 164
MsG0480023528.01.T01 MTR_8g095220 60.417 144 16 1 13 156 155 257 2.80e-48 160
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023528.01.T01 AT5G63620 50.000 144 31 1 13 156 157 259 6.98e-38 133
MsG0480023528.01.T01 AT5G63620 50.000 144 31 1 13 156 157 259 2.46e-37 134
MsG0480023528.01.T01 AT5G63620 50.000 144 31 1 13 156 157 259 2.73e-37 134

Find 42 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAAATTAAGCTTGTCTATA+TGG 0.216639 4:+86485462 None:intergenic
GGTGATACTGTTGCTGTTAT+TGG 0.219498 4:-86485136 MsG0480023528.01.T01:CDS
CTGGCACAAGCTGGTTCTTT+AGG 0.246946 4:-86484469 MsG0480023528.01.T01:CDS
TCGAGTTTCACCATCGTATA+AGG 0.300970 4:+86485577 None:intergenic
ACTGTTGCTGTTATTGGAAC+TGG 0.304388 4:-86485130 MsG0480023528.01.T01:CDS
TATACCGAGTCTGCAATTCT+AGG 0.324055 4:-86485214 MsG0480023528.01.T01:CDS
TCGGTATATGGCATTGAGTT+TGG 0.337585 4:+86485229 None:intergenic
GAAATTTGTATCAGCTGAAT+AGG 0.341589 4:+86485391 None:intergenic
AGAATTGCAGACTCGGTATA+TGG 0.391325 4:+86485217 None:intergenic
CTGTTGCTGTTATTGGAACT+GGG 0.417859 4:-86485129 MsG0480023528.01.T01:CDS
TAAATTAAGCTTGTCTATAT+GGG 0.421177 4:+86485463 None:intergenic
TGAATCAAGTAAGAAGTAAG+AGG 0.421869 4:+86485529 None:intergenic
GGAGGAAAAGCAGTGATGAT+TGG 0.449002 4:-86484493 MsG0480023528.01.T01:CDS
TTTGCTTATAACCGCGCAAA+AGG 0.453633 4:-86485602 MsG0480023528.01.T01:CDS
CGACTGTTTCTTCGAGGAAG+TGG 0.460944 4:-86485557 MsG0480023528.01.T01:CDS
GCTGTCTACAAACGGTATGC+AGG 0.462178 4:-86485651 MsG0480023528.01.T01:CDS
TACAATCATTTCACATGTAT+CGG 0.496233 4:+86485499 None:intergenic
CATGCAGCCGAAGTGCGCCC+TGG 0.499244 4:-86485157 MsG0480023528.01.T01:CDS
AGCTGGTTCTTTAGGAGAGG+TGG 0.503154 4:-86484461 MsG0480023528.01.T01:CDS
TGTCAACTGAATTATGGTCT+CGG 0.516519 4:-86485681 None:intergenic
AAATAGACTTGTTCGCAGAA+AGG 0.523418 4:-86484434 None:intergenic
GAAACTCGACTGTTTCTTCG+AGG 0.525121 4:-86485563 MsG0480023528.01.T01:CDS
GACTATGTAAACACATACTT+AGG 0.531324 4:+86485438 None:intergenic
GTGTTTATGCTGTCTACAAA+CGG 0.542164 4:-86485659 MsG0480023528.01.T01:CDS
GCAAAAGGAACCTTATACGA+TGG 0.560670 4:-86485587 MsG0480023528.01.T01:CDS
TGCACACAAAGTGTGAAAGA+TGG 0.567900 4:-86484514 MsG0480023528.01.T01:CDS
CTGTCTACAAACGGTATGCA+GGG 0.578608 4:-86485650 MsG0480023528.01.T01:CDS
AGTATCACCAGGGCGCACTT+CGG 0.593810 4:+86485150 None:intergenic
ACAAGCTGGTTCTTTAGGAG+AGG 0.597825 4:-86484464 MsG0480023528.01.T01:CDS
TGTTGCTGTTATTGGAACTG+GGG 0.602671 4:-86485128 MsG0480023528.01.T01:CDS
ACATCCTAGAATTGCAGACT+CGG 0.607209 4:+86485210 None:intergenic
TGTGCCAGCAAATGCATTGG+CGG 0.610816 4:-86485257 MsG0480023528.01.T01:intron
ATGATTGGACTGGCACAAGC+TGG 0.611907 4:-86484478 MsG0480023528.01.T01:CDS
GTTGCTGTTATTGGAACTGG+GGG 0.615280 4:-86485127 MsG0480023528.01.T01:CDS
ATAACAGCAACAGTATCACC+AGG 0.620053 4:+86485139 None:intergenic
AAGCTTCGCACAGATCATCA+TGG 0.622065 4:+86485627 None:intergenic
AAAAGCAGTGATGATTGGAC+TGG 0.624735 4:-86484488 MsG0480023528.01.T01:CDS
GAATCAAGTAAGAAGTAAGA+GGG 0.626857 4:+86485530 None:intergenic
TGTTGTGCCAGCAAATGCAT+TGG 0.658264 4:-86485260 MsG0480023528.01.T01:intron
TGTGCACTGAGAAATGTCTG+TGG 0.672742 4:+86484531 None:intergenic
ACACAAAGTGTGAAAGATGG+AGG 0.689218 4:-86484511 MsG0480023528.01.T01:CDS
TAACAGCAACAGTATCACCA+GGG 0.751466 4:+86485140 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATTGGCAAATTTAT+AGG - Chr4:86484775-86484794 MsG0480023528.01.T01:intron 15.0%
!! ATAAATTAAGCTTGTCTATA+TGG + Chr4:86484644-86484663 None:intergenic 20.0%
!! ATTGGAATACTGAAAATAAT+TGG - Chr4:86484763-86484782 MsG0480023528.01.T01:intron 20.0%
!! GAGAGAAAAAACATAAATAA+AGG + Chr4:86485493-86485512 None:intergenic 20.0%
!! TAAATTAAGCTTGTCTATAT+GGG + Chr4:86484643-86484662 None:intergenic 20.0%
!! TTTCAGTATTCCAATTATAA+AGG + Chr4:86484758-86484777 None:intergenic 20.0%
!!! ATATACAAGTATCTTTTCAA+TGG + Chr4:86485352-86485371 None:intergenic 20.0%
!!! TATACAAGTATCTTTTCAAT+GGG + Chr4:86485351-86485370 None:intergenic 20.0%
! GAAACAGCAAAAATAGAAAA+AGG + Chr4:86485075-86485094 None:intergenic 25.0%
! TACAATCATTTCACATGTAT+CGG + Chr4:86484607-86484626 None:intergenic 25.0%
ACCGATATACATGTATAGTA+TGG - Chr4:86484801-86484820 MsG0480023528.01.T01:intron 30.0%
AGAGTAGACTCCTTTATAAT+TGG - Chr4:86484745-86484764 MsG0480023528.01.T01:intron 30.0%
ATATACATGTATAGTATGGG+AGG - Chr4:86484805-86484824 MsG0480023528.01.T01:intron 30.0%
CCCATACTATACATGTATAT+CGG + Chr4:86484805-86484824 None:intergenic 30.0%
CCGATATACATGTATAGTAT+GGG - Chr4:86484802-86484821 MsG0480023528.01.T01:intron 30.0%
GAAATTTGTATCAGCTGAAT+AGG + Chr4:86484715-86484734 None:intergenic 30.0%
GAAGCGTGATAAAGTTAAAA+TGG + Chr4:86485203-86485222 None:intergenic 30.0%
GAATCAAGTAAGAAGTAAGA+GGG + Chr4:86484576-86484595 None:intergenic 30.0%
GACTATGTAAACACATACTT+AGG + Chr4:86484668-86484687 None:intergenic 30.0%
GCAAAAATAGAAAAAGGAAG+TGG + Chr4:86485069-86485088 None:intergenic 30.0%
TATACATGTATAGTATGGGA+GGG - Chr4:86484806-86484825 MsG0480023528.01.T01:intron 30.0%
TGAATCAAGTAAGAAGTAAG+AGG + Chr4:86484577-86484596 None:intergenic 30.0%
TGCAACTCACACAATAAATT+CGG - Chr4:86485314-86485333 MsG0480023528.01.T01:intron 30.0%
TTCGATAATGCTTGCTTAAA+TGG - Chr4:86485092-86485111 MsG0480023528.01.T01:intron 30.0%
! TATAATTGCTGTTGATGTTC+AGG - Chr4:86485262-86485281 MsG0480023528.01.T01:intron 30.0%
ATCAACAGCAATTATATCCG+AGG + Chr4:86485258-86485277 None:intergenic 35.0%
GGTAACTAATCATTGTCAAG+TGG - Chr4:86485010-86485029 MsG0480023528.01.T01:intron 35.0%
GTGTTTATGCTGTCTACAAA+CGG - Chr4:86484444-86484463 MsG0480023528.01.T01:CDS 35.0%
GTTCTGGCAATTAAACAATG+AGG + Chr4:86485389-86485408 None:intergenic 35.0%
TCACACAATAAATTCGGCTA+AGG - Chr4:86485320-86485339 MsG0480023528.01.T01:intron 35.0%
! AGTCATAGTTTTCTCTGCTA+TGG - Chr4:86484684-86484703 MsG0480023528.01.T01:intron 35.0%
! TTTTACTGCTTATGGTGCAA+TGG - Chr4:86484921-86484940 MsG0480023528.01.T01:intron 35.0%
! TTTTCATATATCCGAGCAGT+AGG - Chr4:86485155-86485174 MsG0480023528.01.T01:CDS 35.0%
!!! GTTTTTTCTCTCTCAACTTC+AGG - Chr4:86485501-86485520 MsG0480023528.01.T01:CDS 35.0%
!!! TGTGCTGTTTTTACTGCTTA+TGG - Chr4:86484913-86484932 MsG0480023528.01.T01:intron 35.0%
AAAAGCAGTGATGATTGGAC+TGG - Chr4:86485615-86485634 MsG0480023528.01.T01:CDS 40.0%
ACACAAAGTGTGAAAGATGG+AGG - Chr4:86485592-86485611 MsG0480023528.01.T01:CDS 40.0%
ACATCCTAGAATTGCAGACT+CGG + Chr4:86484896-86484915 None:intergenic 40.0%
AGCTAGAGAAAGCAAACACT+TGG - Chr4:86485291-86485310 MsG0480023528.01.T01:intron 40.0%
ATAACAGCAACAGTATCACC+AGG + Chr4:86484967-86484986 None:intergenic 40.0%
CTCTCAACTTCAGGAAATAC+TGG - Chr4:86485510-86485529 MsG0480023528.01.T01:CDS 40.0%
GCAAAAGGAACCTTATACGA+TGG - Chr4:86484516-86484535 MsG0480023528.01.T01:CDS 40.0%
TAACAGCAACAGTATCACCA+GGG + Chr4:86484966-86484985 None:intergenic 40.0%
TATACCGAGTCTGCAATTCT+AGG - Chr4:86484889-86484908 MsG0480023528.01.T01:intron 40.0%
TCAACTTCAGGAAATACTGG+TGG - Chr4:86485513-86485532 MsG0480023528.01.T01:CDS 40.0%
TCGAGTTTCACCATCGTATA+AGG + Chr4:86484529-86484548 None:intergenic 40.0%
TGCACACAAAGTGTGAAAGA+TGG - Chr4:86485589-86485608 MsG0480023528.01.T01:CDS 40.0%
TGTTAGGAGCTAATAGACAG+AGG + Chr4:86485426-86485445 None:intergenic 40.0%
TTATGCACGATAGTGTGAGT+GGG + Chr4:86485452-86485471 None:intergenic 40.0%
TTCAGGAAATACTGGTGGAA+AGG - Chr4:86485518-86485537 MsG0480023528.01.T01:CDS 40.0%
TTTATGCACGATAGTGTGAG+TGG + Chr4:86485453-86485472 None:intergenic 40.0%
TTTGCTTATAACCGCGCAAA+AGG - Chr4:86484501-86484520 MsG0480023528.01.T01:CDS 40.0%
! ACTGTTGCTGTTATTGGAAC+TGG - Chr4:86484973-86484992 MsG0480023528.01.T01:intron 40.0%
! AGAATTGCAGACTCGGTATA+TGG + Chr4:86484889-86484908 None:intergenic 40.0%
! ATGTTGCTGTTGAAGCCTTA+GGG - Chr4:86485546-86485565 MsG0480023528.01.T01:CDS 40.0%
! CTGTTGCTGTTATTGGAACT+GGG - Chr4:86484974-86484993 MsG0480023528.01.T01:intron 40.0%
! GGTGATACTGTTGCTGTTAT+TGG - Chr4:86484967-86484986 MsG0480023528.01.T01:intron 40.0%
! TCGGTATATGGCATTGAGTT+TGG + Chr4:86484877-86484896 None:intergenic 40.0%
! TGTTGCTGTTATTGGAACTG+GGG - Chr4:86484975-86484994 MsG0480023528.01.T01:intron 40.0%
AAATGTCTGTGGTCTCCCTA+AGG + Chr4:86485564-86485583 None:intergenic 45.0%
AAGCTTCGCACAGATCATCA+TGG + Chr4:86484479-86484498 None:intergenic 45.0%
AATACCGCCAATGCATTTGC+TGG + Chr4:86484853-86484872 None:intergenic 45.0%
CTGTCTACAAACGGTATGCA+GGG - Chr4:86484453-86484472 MsG0480023528.01.T01:CDS 45.0%
GGAGGAAAAGCAGTGATGAT+TGG - Chr4:86485610-86485629 MsG0480023528.01.T01:CDS 45.0%
TAGTGTGAGTGGGTGATGTT+AGG + Chr4:86485442-86485461 None:intergenic 45.0%
TCTAGCTATCTGCAGACAAC+TGG + Chr4:86485225-86485244 None:intergenic 45.0%
TGCCAGAACTCTATCTTGTG+CGG - Chr4:86485400-86485419 MsG0480023528.01.T01:CDS 45.0%
TGTGCACTGAGAAATGTCTG+TGG + Chr4:86485575-86485594 None:intergenic 45.0%
TGTTGTGCCAGCAAATGCAT+TGG - Chr4:86484843-86484862 MsG0480023528.01.T01:intron 45.0%
! ACAAGCTGGTTCTTTAGGAG+AGG - Chr4:86485639-86485658 MsG0480023528.01.T01:CDS 45.0%
! CGTATAAGGTTCCTTTTGCG+CGG + Chr4:86484515-86484534 None:intergenic 45.0%
! CTGCAGATAGCTAGAGCATT+TGG - Chr4:86485230-86485249 MsG0480023528.01.T01:CDS 45.0%
! GAAACTCGACTGTTTCTTCG+AGG - Chr4:86484540-86484559 MsG0480023528.01.T01:intron 45.0%
! GATGTTGCTGTTGAAGCCTT+AGG - Chr4:86485545-86485564 MsG0480023528.01.T01:CDS 45.0%
! GTTGCTGTTATTGGAACTGG+GGG - Chr4:86484976-86484995 MsG0480023528.01.T01:intron 45.0%
GCTGTCTACAAACGGTATGC+AGG - Chr4:86484452-86484471 MsG0480023528.01.T01:CDS 50.0%
GTTGATCAGAGCCTACTGCT+CGG + Chr4:86485169-86485188 None:intergenic 50.0%
TGTGCCAGCAAATGCATTGG+CGG - Chr4:86484846-86484865 MsG0480023528.01.T01:intron 50.0%
! AGCTGGTTCTTTAGGAGAGG+TGG - Chr4:86485642-86485661 MsG0480023528.01.T01:CDS 50.0%
! CGACTGTTTCTTCGAGGAAG+TGG - Chr4:86484546-86484565 MsG0480023528.01.T01:intron 50.0%
! CTGGCACAAGCTGGTTCTTT+AGG - Chr4:86485634-86485653 MsG0480023528.01.T01:CDS 50.0%
! GTTATTGGAACTGGGGGTGT+TGG - Chr4:86484982-86485001 MsG0480023528.01.T01:intron 50.0%
!! AGCTAGAGCATTTGGTGCCT+CGG - Chr4:86485238-86485257 MsG0480023528.01.T01:CDS 50.0%
!! ATGATTGGACTGGCACAAGC+TGG - Chr4:86485625-86485644 MsG0480023528.01.T01:CDS 50.0%
AGTATCACCAGGGCGCACTT+CGG + Chr4:86484956-86484975 None:intergenic 55.0%
GGCCGCACAAGATAGAGTTC+TGG + Chr4:86485405-86485424 None:intergenic 55.0%
!! GAACTGGGGGTGTTGGTTCT+AGG - Chr4:86484989-86485008 MsG0480023528.01.T01:intron 55.0%
CATGCAGCCGAAGTGCGCCC+TGG - Chr4:86484946-86484965 MsG0480023528.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 86484435 86485690 86484435 ID=MsG0480023528.01;Name=MsG0480023528.01
Chr4 mRNA 86484435 86485690 86484435 ID=MsG0480023528.01.T01;Parent=MsG0480023528.01;Name=MsG0480023528.01.T01;_AED=0.41;_eAED=0.48;_QI=0|0|0|1|0.5|0|3|0|198
Chr4 exon 86485378 86485690 86485378 ID=MsG0480023528.01.T01:exon:3094;Parent=MsG0480023528.01.T01
Chr4 exon 86485115 86485271 86485115 ID=MsG0480023528.01.T01:exon:3093;Parent=MsG0480023528.01.T01
Chr4 exon 86484435 86484558 86484435 ID=MsG0480023528.01.T01:exon:3092;Parent=MsG0480023528.01.T01
Chr4 CDS 86485378 86485690 86485378 ID=MsG0480023528.01.T01:cds;Parent=MsG0480023528.01.T01
Chr4 CDS 86485115 86485271 86485115 ID=MsG0480023528.01.T01:cds;Parent=MsG0480023528.01.T01
Chr4 CDS 86484435 86484558 86484435 ID=MsG0480023528.01.T01:cds;Parent=MsG0480023528.01.T01
Gene Sequence

>MsG0480023528.01.T01

ATGGTCTCGGTGTTTATGCTGTCTACAAACGGTATGCAGGGCCATGATGATCTGTGCGAAGCTTTTTTTGCTTATAACCGCGCAAAAGGAACCTTATACGATGGTGAAACTCGACTGTTTCTTCGAGGAAGTGGTGAACCCTCTTACTTCTTACTTGATTCACATATTACCGATACATGTGAAATGATTGTATGTTATCATTATTCCCATATAGACAAGCTTAATTTATACCTAAGTATGTGTTTACATAGTCATAGTTTTCTCTGCTATGGTATTTCCTATTCAGCTGATACAAATTTCATATTTGTTGAGACAAATGCATTGGCGGTATTGCCAAACTCAATGCCATATACCGAGTCTGCAATTCTAGGATGTGCTGTTTTTACTGCTTATGGTGCAATGGCTCATGCAGCCGAAGTGCGCCCTGGTGATACTGTTGCTGTTATTGGAACTGGGGGTGTTGGTTCTAGGGAGACCACAGACATTTCTCAGTGCACACAAAGTGTGAAAGATGGAGGAAAAGCAGTGATGATTGGACTGGCACAAGCTGGTTCTTTAGGAGAGGTGGATATAAATAGACTTGTTCGCAGAAAG

Protein sequence

>MsG0480023528.01.T01

MVSVFMLSTNGMQGHDDLCEAFFAYNRAKGTLYDGETRLFLRGSGEPSYFLLDSHITDTCEMIVCYHYSHIDKLNLYLSMCLHSHSFLCYGISYSADTNFIFVETNALAVLPNSMPYTESAILGCAVFTAYGAMAHAAEVRPGDTVAVIGTGGVGSRETTDISQCTQSVKDGGKAVMIGLAQAGSLGEVDINRLVRRK