AlfalfaGEDB | MsG0480023636.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023636.01

KEGG: K00432
GO: GO:0004602 GO:0006979 GO:0055114
PFAM: PF00255

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023636.01.T01	XP_003630518.1	97.093	172	5	0	1	172	1	172	1.37E-117	340

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023636.01.T01	Q8LBU2	77.439	164	37	0	8	171	3	166	7.39E-95	275

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023636.01.T01	G7LHZ7	97.093	172	5	0	1	172	1	172	6.56e-118	340

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047869.01	MsG0480023636.01	0.845464	3.996577e-59	6.665955e-56
MsG0080047957.01	MsG0480023636.01	0.802669	5.028405e-49	2.477635e-46
MsG0080047959.01	MsG0480023636.01	0.801868	7.349677e-49	3.548483e-46
MsG0080048113.01	MsG0480023636.01	0.815810	7.640586e-52	5.321829e-49
MsG0080048404.01	MsG0480023636.01	0.823446	1.379482e-53	1.187990e-50
MsG0080048461.01	MsG0480023636.01	0.804833	1.787077e-49	9.309047e-47
MsG0080048462.01	MsG0480023636.01	0.814428	1.548150e-51	1.038550e-48
MsG0080048600.01	MsG0480023636.01	0.847064	1.464471e-59	2.572026e-56
MsG0080048618.01	MsG0480023636.01	0.832186	1.091209e-55	1.210548e-52
MsG0080048774.01	MsG0480023636.01	0.819740	9.909942e-53	7.690680e-50

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023636.01.T01	MTR_8g098400	97.093	172	5	0	1	172	1	172	1.66e-121	340
MsG0480023636.01.T01	MTR_8g098400	96.914	162	5	0	1	162	1	162	2.21e-113	320
MsG0480023636.01.T01	MTR_8g098400	97.600	125	3	0	48	172	1	125	3.67e-87	251
MsG0480023636.01.T01	MTR_8g098410	68.902	164	51	0	6	169	2	165	7.48e-87	252
MsG0480023636.01.T01	MTR_8g098410	68.902	164	51	0	6	169	68	231	8.08e-87	254
MsG0480023636.01.T01	MTR_5g010340	71.257	167	47	1	5	170	3	169	1.15e-83	245
MsG0480023636.01.T01	MTR_8g105630	67.089	158	52	0	12	169	7	164	1.47e-77	228
MsG0480023636.01.T01	MTR_1g014210	64.375	160	57	0	12	171	77	236	3.28e-75	225
MsG0480023636.01.T01	MTR_7g094600	60.606	165	65	0	6	170	4	168	4.10e-73	217
MsG0480023636.01.T01	MTR_8g098400	94.393	107	6	0	1	107	1	107	1.56e-69	207

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023636.01.T01	AT1G63460	77.439	164	37	8	171	3	166	7.54e-96	275
MsG0480023636.01.T01	AT4G11600	73.494	166	44	6	171	65	230	1.08e-91	267
MsG0480023636.01.T01	AT4G31870	66.875	160	53	12	171	74	233	9.68e-77	229
MsG0480023636.01.T01	AT2G43350	62.722	169	63	2	170	36	204	1.34e-74	223
MsG0480023636.01.T01	AT2G43350	62.722	169	63	2	170	36	204	1.34e-74	223
MsG0480023636.01.T01	AT2G31570	64.815	162	57	9	170	4	165	1.38e-74	221
MsG0480023636.01.T01	AT2G25080	63.125	160	59	12	171	77	236	4.59e-73	219
MsG0480023636.01.T01	AT3G63080	59.119	159	65	14	172	14	172	1.27e-69	209
MsG0480023636.01.T01	AT2G48150	56.098	164	72	6	169	4	167	3.64e-66	200
MsG0480023636.01.T01	AT2G48150	58.156	141	59	29	169	1	141	1.25e-58	180

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Find 68 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCAATATTTGATCAT+TGG	0.340942	4:-87950289	None:intergenic
AAACTCCATTACCTCTGTTT+CGG	0.263664	4:+87949436	MsG0480023636.01.T01:five_prime_UTR
AACTCCATTACCTCTGTTTC+GGG	0.246672	4:+87949437	MsG0480023636.01.T01:five_prime_UTR
AAGCAAACATGTTGTCTATA+AGG	0.304042	4:-87951394	None:intergenic
AAGCAACATTAACAATGAGG+AGG	0.704587	4:-87949716	None:intergenic
AAGGCTAAGAGGAGAGGTGG+TGG	0.529913	4:-87951077	None:intergenic
AAGTTTCTAGTTGACAAGGA+TGG	0.601035	4:+87951031	MsG0480023636.01.T01:CDS
AATATTTGATCATTGGTTCC+AGG	0.413256	4:-87950282	None:intergenic
AATTCTTAAAGTCAGGTAAA+TGG	0.245816	4:+87950972	MsG0480023636.01.T01:CDS
ACCGTACTCACTTGACTTGA+TGG	0.356237	4:-87949482	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAACGCTAATTTAGGT+TGG	-	Chr4:87950135-87950154	None:intergenic	30.0%
AAGCAAACATGTTGTCTATA+AGG	-	Chr4:87951397-87951416	None:intergenic	30.0%
ACACATCAATTGACACAATT+TGG	+	Chr4:87949793-87949812	MsG0480023636.01.T01:intron	30.0%
CAGCTTAAAGTATAAGTGTA+CGG	-	Chr4:87949967-87949986	None:intergenic	30.0%
GAATTACCTTATCAAAGATG+GGG	-	Chr4:87950341-87950360	None:intergenic	30.0%
GCATTTCCAAGTAATCAATT+TGG	+	Chr4:87950249-87950268	MsG0480023636.01.T01:CDS	30.0%
GTGTACGGATAAGTTATTTA+TGG	-	Chr4:87949952-87949971	None:intergenic	30.0%
TAACAGTAAGGATCCAAAAT+CGG	+	Chr4:87949528-87949547	MsG0480023636.01.T01:CDS	30.0%
TAACTCCACATAATTTGAGT+TGG	-	Chr4:87950056-87950075	None:intergenic	30.0%
TATGACAAGTACAAACACAA+AGG	+	Chr4:87950085-87950104	MsG0480023636.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	87949404	87951495	87949404	ID=MsG0480023636.01;Name=MsG0480023636.01
Chr4	mRNA	87949404	87951495	87949404	ID=MsG0480023636.01.T01;Parent=MsG0480023636.01;Name=MsG0480023636.01.T01;_AED=0.44;_eAED=0.44;_QI=107\|0.6\|0.5\|1\|0.8\|0.83\|6\|246\|172
Chr4	exon	87949404	87949570	87949404	ID=MsG0480023636.01.T01:exon:3242;Parent=MsG0480023636.01.T01
Chr4	exon	87949669	87949745	87949669	ID=MsG0480023636.01.T01:exon:3243;Parent=MsG0480023636.01.T01
Chr4	exon	87950045	87950106	87950045	ID=MsG0480023636.01.T01:exon:3244;Parent=MsG0480023636.01.T01
Chr4	exon	87950235	87950353	87950235	ID=MsG0480023636.01.T01:exon:3245;Parent=MsG0480023636.01.T01
Chr4	exon	87950935	87951102	87950935	ID=MsG0480023636.01.T01:exon:3246;Parent=MsG0480023636.01.T01
Chr4	exon	87951217	87951495	87951217	ID=MsG0480023636.01.T01:exon:3247;Parent=MsG0480023636.01.T01
Chr4	five_prime_UTR	87949404	87949510	87949404	ID=MsG0480023636.01.T01:five_prime_utr;Parent=MsG0480023636.01.T01
Chr4	CDS	87949511	87949570	87949511	ID=MsG0480023636.01.T01:cds;Parent=MsG0480023636.01.T01
Chr4	CDS	87949669	87949745	87949669	ID=MsG0480023636.01.T01:cds;Parent=MsG0480023636.01.T01
Chr4	CDS	87950045	87950106	87950045	ID=MsG0480023636.01.T01:cds;Parent=MsG0480023636.01.T01
Chr4	CDS	87950235	87950353	87950235	ID=MsG0480023636.01.T01:cds;Parent=MsG0480023636.01.T01
Chr4	CDS	87950935	87951102	87950935	ID=MsG0480023636.01.T01:cds;Parent=MsG0480023636.01.T01
Chr4	CDS	87951217	87951249	87951217	ID=MsG0480023636.01.T01:cds;Parent=MsG0480023636.01.T01
Chr4	three_prime_UTR	87951250	87951495	87951250	ID=MsG0480023636.01.T01:three_prime_utr;Parent=MsG0480023636.01.T01

Gene Sequence

>MsG0480023636.01.T01

ATGAGCAGCGAACCCAGTAACAGTAAGGATCCAAAATCGGTCTACGACTTCACTCTCAAGGATGGTAAGGGAAATGATGTTGATCTCGCCACTTACAAGGGTAAAGTCCTCCTCATTGTTAATGTTGCTTCCAAATGTGGCATGACCAACTCAAATTATGTGGAGTTAAATCAACTATATGACAAGTACAAACACAAAGGCCTTGAGATTCTGGCATTTCCAAGTAATCAATTTGGCGAGGAAGAACCTGGAACCAATGATCAAATATTGGATTTTGTTTGTACTCGCTTCAAATCAGAATTCCCCATCTTTGATAAGATTGAAGTGAATGGGGACAATTCTGCTCCCTTGTATAAATTCTTAAAGTCAGGTAAATGGGGAATTTTTGGGGATGATATTCAGTGGAACTTTGCCAAGTTTCTAGTTGACAAGGATGGTCAAGTGGTGGATCGATATTATCCCACCACCTCTCCTCTTAGCCTTGAGCGAGACATCTGTAAACTACTTGGGGTTGAATGA

Protein sequence

>MsG0480023636.01.T01

MSSEPSNSKDPKSVYDFTLKDGKGNDVDLATYKGKVLLIVNVASKCGMTNSNYVELNQLYDKYKHKGLEILAFPSNQFGEEEPGTNDQILDFVCTRFKSEFPIFDKIEVNGDNSAPLYKFLKSGKWGIFGDDIQWNFAKFLVDKDGQVVDRYYPTTSPLSLERDICKLLGVE*