AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048113.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 102 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGTACGATGCTTGTTATTT+TGG 0.166397 contig211end:-469 MsG0080048113.01.T01:three_prime_UTR
TGAAATAAATTGAATACTAT+TGG 0.224630 contig211end:+295 None:intergenic
GAAAATTCTAGTTTAGACTA+TGG 0.287489 contig211end:-496 MsG0080048113.01.T01:three_prime_UTR
GAAATAAATTGAATACTATT+GGG 0.333612 contig211end:+296 None:intergenic
GACATCATCAAGACAGTTCC+TGG 0.394800 contig211end:-584 MsG0080048113.01.T01:CDS
TTTGCGGCCACCATTGTTGA+AGG 0.420354 contig211end:+2263 None:intergenic
TTACTCAATGCTCAATCAGC+TGG 0.426863 contig211end:+441 None:intergenic
GGCCACCATTGTTGAAGGTT+TGG 0.449324 contig211end:+2268 None:intergenic
AATTACCTTTGGAACTCCTA+AGG 0.463721 contig211end:+2046 None:intergenic
TGCCTCAGCATTGTTGATCC+TGG 0.466033 contig211end:-696 MsG0080048113.01.T01:intron
AACATAGAATTGAAAACTCA+TGG 0.469063 contig211end:+537 None:intergenic
GAAGCTTACGGTCTTCTTTG+CGG 0.470686 contig211end:+2247 None:intergenic
TGTTGACTTGGCACTGCTTG+TGG 0.471947 contig211end:-738 MsG0080048113.01.T01:CDS
TCATGAGAGCACATCACAAC+AGG 0.473544 contig211end:-2126 MsG0080048113.01.T01:CDS
CTTTACGTTTACTGATCTCC+AGG 0.475814 contig211end:+566 None:intergenic
ATTACCTTTGGAACTCCTAA+GGG 0.478678 contig211end:+2047 None:intergenic
AAAGTGTAAACACTGCCTCT+AGG 0.491840 contig211end:+2330 None:intergenic
AAAATTCTAGTTTAGACTAT+GGG 0.495425 contig211end:-495 MsG0080048113.01.T01:three_prime_UTR
GCTTAGTTTGATGATTGCTA+TGG 0.496618 contig211end:-943 MsG0080048113.01.T01:CDS
TCTAGTTTAGACTATGGGTA+CGG 0.510235 contig211end:-490 MsG0080048113.01.T01:three_prime_UTR
AAACTGTTCTCAAATCCCTT+AGG 0.514891 contig211end:-2062 MsG0080048113.01.T01:CDS
TGAAGAGTGGAAATATACCC+TGG 0.520988 contig211end:-2090 MsG0080048113.01.T01:CDS
TAGTATTCAATTTATTTCAG+TGG 0.522114 contig211end:-291 MsG0080048113.01.T01:three_prime_UTR
CTCGCTCTTGTCATGAAGAG+TGG 0.545906 contig211end:-2103 MsG0080048113.01.T01:CDS
ATAGTAACAAGTGCTGATCA+AGG 0.550433 contig211end:+916 None:intergenic
TACTCAATGCTCAATCAGCT+GGG 0.550544 contig211end:+442 None:intergenic
CTAGTTTAGACTATGGGTAC+GGG 0.560538 contig211end:-489 MsG0080048113.01.T01:three_prime_UTR
CTATATAGAAATTAGAAACA+TGG 0.570635 contig211end:+398 None:intergenic
TATCATAGACAATGTTGACT+TGG 0.578483 contig211end:-750 MsG0080048113.01.T01:intron
TCACACCAAACCTTCAACAA+TGG 0.594420 contig211end:-2273 MsG0080048113.01.T01:exon
AGGAACTGTCTTGATGATGT+CGG 0.599851 contig211end:+586 None:intergenic
TATTCAATTTATTTCAGTGG+AGG 0.601690 contig211end:-288 MsG0080048113.01.T01:three_prime_UTR
ACATAGAATTGAAAACTCAT+GGG 0.602698 contig211end:+538 None:intergenic
AAATCCCTTAGGAGTTCCAA+AGG 0.605938 contig211end:-2051 MsG0080048113.01.T01:intron
CACCAAACCTTCAACAATGG+TGG 0.615697 contig211end:-2270 MsG0080048113.01.T01:exon
GAAGAGTGGAAATATACCCT+GGG 0.633856 contig211end:-2089 MsG0080048113.01.T01:CDS
TACCAGGATCAACAATGCTG+AGG 0.662178 contig211end:+694 None:intergenic
GATGCGCAGAGAAGAAAATG+CGG 0.718290 contig211end:+2296 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTGAAAAAATATTATACAT+GGG - contig211end:1374-1393 MsG0080048113.01.T01:intron 15.0%
!! GAAATAAATTGAATACTATT+GGG + contig211end:2316-2335 None:intergenic 15.0%
!! TACTGAAAAAATATTATACA+TGG - contig211end:1373-1392 MsG0080048113.01.T01:intron 15.0%
!! TGAAATAAATTGAATACTAT+TGG + contig211end:2317-2336 None:intergenic 15.0%
!!! AACTTTATTTTATAAGATTC+TGG - contig211end:1447-1466 MsG0080048113.01.T01:intron 15.0%
!! ATACATATTTCAACAACAAA+TGG + contig211end:597-616 None:intergenic 20.0%
!! ATTGAAAAATTAATGACACA+GGG + contig211end:982-1001 None:intergenic 20.0%
!! CCAAATAACAAATAAATTAG+AGG + contig211end:1988-2007 None:intergenic 20.0%
!! CTATATAGAAATTAGAAACA+TGG + contig211end:2214-2233 None:intergenic 20.0%
!! TAGTATTCAATTTATTTCAG+TGG - contig211end:2318-2337 MsG0080048113.01.T01:five_prime_UTR 20.0%
!! TATGTTTGGATTAATGATAT+CGG + contig211end:1217-1236 None:intergenic 20.0%
!! TGGATAAAAAAAATTACCTT+TGG + contig211end:577-596 None:intergenic 20.0%
!! TGTTGAAAGAATATTATACA+TGG - contig211end:1319-1338 MsG0080048113.01.T01:intron 20.0%
!! TTAATCCAAACATAATGTTA+TGG - contig211end:1223-1242 MsG0080048113.01.T01:intron 20.0%
!!! AAAATTCTAGTTTAGACTAT+GGG - contig211end:2114-2133 MsG0080048113.01.T01:CDS 20.0%
!!! AATATTTTTTCAGTATGACA+GGG + contig211end:1368-1387 None:intergenic 20.0%
!!! AGCTAGTTATACTTTAAAAA+TGG - contig211end:1159-1178 MsG0080048113.01.T01:intron 20.0%
!!! CCTCTAATTTATTTGTTATT+TGG - contig211end:1985-2004 MsG0080048113.01.T01:intron 20.0%
!!! GATTTTGATTTGTTATTGTA+GGG - contig211end:832-851 MsG0080048113.01.T01:intron 20.0%
!!! TAATATTTTTTCAGTATGAC+AGG + contig211end:1369-1388 None:intergenic 20.0%
!!! TATAATTTTCTATCAGTAGT+TGG - contig211end:1491-1510 MsG0080048113.01.T01:intron 20.0%
!!! TGATTTTGATTTGTTATTGT+AGG - contig211end:831-850 MsG0080048113.01.T01:intron 20.0%
!!! TTATTTGATGATTATGCATA+TGG - contig211end:1618-1637 MsG0080048113.01.T01:intron 20.0%
!!! TTGATTGAATTAAGTGATTT+TGG - contig211end:775-794 MsG0080048113.01.T01:intron 20.0%
!!! TTTGTTGTTGAAATATGTAT+TGG - contig211end:597-616 MsG0080048113.01.T01:intron 20.0%
! AAATAAGTGATTAGTGAAAG+TGG - contig211end:729-748 MsG0080048113.01.T01:CDS 25.0%
! AACATAGAATTGAAAACTCA+TGG + contig211end:2075-2094 None:intergenic 25.0%
! ACATAGAATTGAAAACTCAT+GGG + contig211end:2074-2093 None:intergenic 25.0%
! ATGTTTGGATTAATGATATC+GGG + contig211end:1216-1235 None:intergenic 25.0%
! CATTGAAAAATTAATGACAC+AGG + contig211end:983-1002 None:intergenic 25.0%
! TAGAATGAAATGAAATGTAG+TGG - contig211end:1119-1138 MsG0080048113.01.T01:intron 25.0%
! TATGAATTGATTAGATTGCA+AGG - contig211end:642-661 MsG0080048113.01.T01:intron 25.0%
! TATGATTTCGTTCAGATTTA+GGG - contig211end:413-432 MsG0080048113.01.T01:three_prime_UTR 25.0%
! TATTCAATTTATTTCAGTGG+AGG - contig211end:2321-2340 MsG0080048113.01.T01:five_prime_UTR 25.0%
! TTATGATTTCGTTCAGATTT+AGG - contig211end:412-431 MsG0080048113.01.T01:three_prime_UTR 25.0%
!! AAACAACTTTTCCATTGTAA+TGG + contig211end:943-962 None:intergenic 25.0%
!! TACATTAACAGAAGCATATT+TGG + contig211end:1549-1568 None:intergenic 25.0%
!!! ATTTGTTATTTGGTGTTTTC+AGG - contig211end:1995-2014 MsG0080048113.01.T01:intron 25.0%
!!! GAAAATTCTAGTTTAGACTA+TGG - contig211end:2113-2132 MsG0080048113.01.T01:CDS 25.0%
!!! TGAAATGTAGTGGATTAAAA+TGG - contig211end:1129-1148 MsG0080048113.01.T01:intron 25.0%
AATTATTGCATGTGACCATA+AGG + contig211end:1276-1295 None:intergenic 30.0%
AATTGAGTACTATGCTATGT+TGG - contig211end:902-921 MsG0080048113.01.T01:intron 30.0%
ATTATTGCATGTGACCATAA+GGG + contig211end:1275-1294 None:intergenic 30.0%
ATTGTGTATGTATACCCTTA+TGG - contig211end:1258-1277 MsG0080048113.01.T01:intron 30.0%
ATTGTTATCTCCAAATCGTT+GGG - contig211end:1725-1744 MsG0080048113.01.T01:intron 30.0%
TACTGAATGAAAGAAGCTTA+CGG + contig211end:377-396 None:intergenic 30.0%
TATTGTTATCTCCAAATCGT+TGG - contig211end:1724-1743 MsG0080048113.01.T01:intron 30.0%
TGTGACCATAACATTATGTT+TGG + contig211end:1231-1250 None:intergenic 30.0%
TTAAGAAAGCAACATATACC+AGG + contig211end:1934-1953 None:intergenic 30.0%
! TATCATAGACAATGTTGACT+TGG - contig211end:1859-1878 MsG0080048113.01.T01:intron 30.0%
!! GTTGTAGATTGATTTTGTAG+TGG - contig211end:706-725 MsG0080048113.01.T01:CDS 30.0%
!! TTCAATGTTGATAGCATTCA+GGG - contig211end:996-1015 MsG0080048113.01.T01:intron 30.0%
!! TTTCAATGTTGATAGCATTC+AGG - contig211end:995-1014 MsG0080048113.01.T01:intron 30.0%
!! TTTGAATGTTGATTTGGTAG+TGG - contig211end:801-820 MsG0080048113.01.T01:intron 30.0%
!!! TGGAGTTTTGAATGTTGATT+TGG - contig211end:795-814 MsG0080048113.01.T01:intron 30.0%
AAACTGTTCTCAAATCCCTT+AGG - contig211end:547-566 MsG0080048113.01.T01:CDS 35.0%
ATAGCATTCAGGGATTGTTT+AGG - contig211end:1006-1025 MsG0080048113.01.T01:intron 35.0%
ATTAATGACACAGGGAAGTA+AGG + contig211end:974-993 None:intergenic 35.0%
TAAGAAGAATCCCAACGATT+TGG + contig211end:1738-1757 None:intergenic 35.0%
TCAATTTCAGACTTCCTCAA+AGG + contig211end:888-907 None:intergenic 35.0%
TTTCTATCAGTAGTTGGATG+TGG - contig211end:1497-1516 MsG0080048113.01.T01:intron 35.0%
TTTGGATTAATGATATCGGG+TGG + contig211end:1213-1232 None:intergenic 35.0%
! CAGAAGCATATTTGGAAACA+AGG + contig211end:1541-1560 None:intergenic 35.0%
! GCTTAGTTTGATGATTGCTA+TGG - contig211end:1666-1685 MsG0080048113.01.T01:intron 35.0%
! TCTAGTTTAGACTATGGGTA+CGG - contig211end:2119-2138 MsG0080048113.01.T01:CDS 35.0%
! TCTGTTATCAGTTGACTCTA+AGG - contig211end:1783-1802 MsG0080048113.01.T01:intron 35.0%
!! AATTACCTTTGGAACTCCTA+AGG + contig211end:566-585 None:intergenic 35.0%
!! ATAGTAACAAGTGCTGATCA+AGG + contig211end:1696-1715 None:intergenic 35.0%
!! ATTACCTTTGGAACTCCTAA+GGG + contig211end:565-584 None:intergenic 35.0%
!! TGAGAACAGTTTTATACCCA+GGG + contig211end:539-558 None:intergenic 35.0%
!! TTGAGAACAGTTTTATACCC+AGG + contig211end:540-559 None:intergenic 35.0%
!!! GGTACGATGCTTGTTATTTT+GGG - contig211end:2141-2160 MsG0080048113.01.T01:intron 35.0%
AAATCCCTTAGGAGTTCCAA+AGG - contig211end:558-577 MsG0080048113.01.T01:CDS 40.0%
AAATGACACTACTGTACGAC+AGG + contig211end:1422-1441 None:intergenic 40.0%
AATGACACTACTGTACGACA+GGG + contig211end:1421-1440 None:intergenic 40.0%
ATTTCAGACTTCCTCAAAGG+AGG + contig211end:885-904 None:intergenic 40.0%
CTAGTTTAGACTATGGGTAC+GGG - contig211end:2120-2139 MsG0080048113.01.T01:CDS 40.0%
CTTTACGTTTACTGATCTCC+AGG + contig211end:2046-2065 None:intergenic 40.0%
GAAGAGTGGAAATATACCCT+GGG - contig211end:520-539 MsG0080048113.01.T01:exon 40.0%
GTTGGAGTTCACCATTACAA+TGG - contig211end:929-948 MsG0080048113.01.T01:CDS 40.0%
TACTCAATGCTCAATCAGCT+GGG + contig211end:2170-2189 None:intergenic 40.0%
TCACACCAAACCTTCAACAA+TGG - contig211end:336-355 MsG0080048113.01.T01:three_prime_UTR 40.0%
TCAGCTAGCTAGAATCAATC+AGG + contig211end:1077-1096 None:intergenic 40.0%
TGAAGAGTGGAAATATACCC+TGG - contig211end:519-538 MsG0080048113.01.T01:exon 40.0%
TTACTCAATGCTCAATCAGC+TGG + contig211end:2171-2190 None:intergenic 40.0%
! AGTACTATGCTATGTTGGCT+AGG - contig211end:907-926 MsG0080048113.01.T01:CDS 40.0%
! TGAGCTTTAGTGTGTATCAG+TGG - contig211end:1094-1113 MsG0080048113.01.T01:intron 40.0%
!! AGGAACTGTCTTGATGATGT+CGG + contig211end:2026-2045 None:intergenic 40.0%
!!! GGGTACGATGCTTGTTATTT+TGG - contig211end:2140-2159 MsG0080048113.01.T01:intron 40.0%
CACCAAACCTTCAACAATGG+TGG - contig211end:339-358 MsG0080048113.01.T01:three_prime_UTR 45.0%
CTAACAACTGTCCTCCTTTG+AGG - contig211end:871-890 MsG0080048113.01.T01:intron 45.0%
GAAGCTTACGGTCTTCTTTG+CGG + contig211end:365-384 None:intergenic 45.0%
GACATCATCAAGACAGTTCC+TGG - contig211end:2025-2044 MsG0080048113.01.T01:intron 45.0%
GATGCGCAGAGAAGAAAATG+CGG + contig211end:316-335 None:intergenic 45.0%
TACCAGGATCAACAATGCTG+AGG + contig211end:1918-1937 None:intergenic 45.0%
! CTATGCTATGTTGGCTAGGT+TGG - contig211end:911-930 MsG0080048113.01.T01:CDS 45.0%
! TCATGAGAGCACATCACAAC+AGG - contig211end:483-502 MsG0080048113.01.T01:three_prime_UTR 45.0%
CTCGCTCTTGTCATGAAGAG+TGG - contig211end:506-525 MsG0080048113.01.T01:three_prime_UTR 50.0%
GGCCACCATTGTTGAAGGTT+TGG + contig211end:344-363 None:intergenic 50.0%
TGCCTCAGCATTGTTGATCC+TGG - contig211end:1913-1932 MsG0080048113.01.T01:intron 50.0%
TTTGCGGCCACCATTGTTGA+AGG + contig211end:349-368 None:intergenic 50.0%
!! TGTTGACTTGGCACTGCTTG+TGG - contig211end:1871-1890 MsG0080048113.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
contig211end gene 282 2349 282 ID=MsG0080048113.01;Name=MsG0080048113.01
contig211end mRNA 282 2349 282 ID=MsG0080048113.01.T01;Parent=MsG0080048113.01;Name=MsG0080048113.01.T01;_AED=0.31;_eAED=0.31;_QI=73|0.5|0.8|0.8|0.25|0.4|5|249|119
contig211end exon 2256 2349 2256 ID=MsG0080048113.01.T01:exon:26416;Parent=MsG0080048113.01.T01
contig211end exon 2052 2156 2052 ID=MsG0080048113.01.T01:exon:26417;Parent=MsG0080048113.01.T01
contig211end exon 904 985 904 ID=MsG0080048113.01.T01:exon:26418;Parent=MsG0080048113.01.T01
contig211end exon 697 764 697 ID=MsG0080048113.01.T01:exon:26419;Parent=MsG0080048113.01.T01
contig211end exon 282 614 282 ID=MsG0080048113.01.T01:exon:26420;Parent=MsG0080048113.01.T01
contig211end five_prime_UTR 2277 2349 2277 ID=MsG0080048113.01.T01:five_prime_utr;Parent=MsG0080048113.01.T01
contig211end CDS 2256 2276 2256 ID=MsG0080048113.01.T01:cds;Parent=MsG0080048113.01.T01
contig211end CDS 2052 2156 2052 ID=MsG0080048113.01.T01:cds;Parent=MsG0080048113.01.T01
contig211end CDS 904 985 904 ID=MsG0080048113.01.T01:cds;Parent=MsG0080048113.01.T01
contig211end CDS 697 764 697 ID=MsG0080048113.01.T01:cds;Parent=MsG0080048113.01.T01
contig211end CDS 531 614 531 ID=MsG0080048113.01.T01:cds;Parent=MsG0080048113.01.T01
contig211end three_prime_UTR 282 530 282 ID=MsG0080048113.01.T01:three_prime_utr;Parent=MsG0080048113.01.T01
Gene Sequence

>MsG0080048113.01.T01

ATGGTGGCCGCAAAGAAGACCAAGAAGACTCATGAGAGCACATCACAACAGGCTCGCTCTTGTCATGAAGAGTGGAAATATACCCTGGGTATAAAACTGTTCTCAAATCCCTTAGGAGTTCCAAAGTTTGATGATTATGCATATATGCTTAGTTTGATGATTGCTATGGCTTACCTTGATCAGCACTTGTTACTATTCTTGTTGTATTACAATGTTGACTTGGCACTGCTTGTGGAAAGTACTACAGAGTGTGCTGCCTCAGCATTGTTGATCCTGGTGATTCCGACATCATCAAGACAGTTCCTGGAGATCAGTAAACGTAAAGATTTTCCCATGAGTTTTCAATTCTATGTTGTTTAG

Protein sequence

>MsG0080048113.01.T01

MVAAKKTKKTHESTSQQARSCHEEWKYTLGIKLFSNPLGVPKFDDYAYMLSLMIAMAYLDQHLLLFLLYYNVDLALLVESTTECAASALLILVIPTSSRQFLEISKRKDFPMSFQFYVV*