AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048404.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048404.01.T01 MTR_3g435250 91.566 83 7 0 40 122 115 197 1.40e-49 156
MsG0080048404.01.T01 MTR_3g435230 79.518 83 17 0 40 122 115 197 1.43e-41 136
MsG0080048404.01.T01 MTR_3g435200 68.675 83 26 0 40 122 113 195 1.97e-36 123
MsG0080048404.01.T01 MTR_3g435170 77.465 71 16 0 40 110 115 185 1.29e-34 118
MsG0080048404.01.T01 MTR_5g041930 55.422 83 37 0 40 122 120 202 5.15e-30 107
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048404.01.T01 AT1G18720 60.000 90 36 0 31 120 113 202 4.70e-33 115
MsG0080048404.01.T01 AT1G18720 60.000 90 36 0 31 120 113 202 4.70e-33 115
MsG0080048404.01.T01 AT1G74440 58.974 78 32 0 38 115 120 197 4.77e-28 102
MsG0080048404.01.T01 AT1G74440 58.974 78 32 0 38 115 120 197 4.77e-28 102

Find 46 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCCTTCAATTCTTTGATAT+TGG 0.256590 contig304end:-4750 None:intergenic
TGTCTTTATTGAGGGGAAAT+TGG 0.315216 contig304end:+4941 MsG0080048404.01.T01:intron
TTGAAATAGATAATCTTTGC+TGG 0.350738 contig304end:+5222 MsG0080048404.01.T01:three_prime_UTR
GCTGTTATAAACCATTCCTT+TGG 0.358951 contig304end:+4816 MsG0080048404.01.T01:three_prime_UTR
TTTGTCTGAAATCCAGGATA+TGG 0.368016 contig304end:-4707 None:intergenic
TAGCTAGACTCTGCTCCTTT+AGG 0.375909 contig304end:+3977 MsG0080048404.01.T01:CDS
GTGTTGGCTGCTCAGTTGAT+TGG 0.382102 contig304end:+4393 MsG0080048404.01.T01:CDS
TGTTGTGTTGTCATTTATTG+AGG 0.416812 contig304end:+4874 MsG0080048404.01.T01:three_prime_UTR
CTCAGACTCCATCTCGAATA+CGG 0.422561 contig304end:+3953 MsG0080048404.01.T01:CDS
AGCCAATATCAAAGAATTGA+AGG 0.427037 contig304end:+4748 MsG0080048404.01.T01:CDS
TTCACCTTTGTCTGAAATCC+AGG 0.431535 contig304end:-4713 None:intergenic
TATAATCTTTCAGGTTGTGT+TGG 0.431542 contig304end:+4377 MsG0080048404.01.T01:intron
GGATGGACTGGAACATCTAT+TGG 0.439686 contig304end:+4414 MsG0080048404.01.T01:CDS
GTTCATATCCAATGGATGAT+TGG 0.446343 contig304end:-4685 None:intergenic
ATTGGATATGAACCATATCC+TGG 0.460320 contig304end:+4695 MsG0080048404.01.T01:CDS
TATTGGTCATGGCGTGTTTG+AGG 0.463494 contig304end:+4431 MsG0080048404.01.T01:CDS
TGGCTGCTCAGTTGATTGGA+TGG 0.484630 contig304end:+4397 MsG0080048404.01.T01:CDS
ACCTCAAGAATCACGAAGAA+AGG 0.495722 contig304end:-4583 None:intergenic
GCTCAGTTGATTGGATGGAC+TGG 0.499989 contig304end:+4402 MsG0080048404.01.T01:CDS
CTACATTGACAACGAAATTG+AGG 0.502607 contig304end:-5048 None:intergenic
CAACACTGATTCTTCTCAAG+AGG 0.505881 contig304end:+5095 MsG0080048404.01.T01:three_prime_UTR
CGATTGGATGACCTTGCTCA+AGG 0.509060 contig304end:+4547 MsG0080048404.01.T01:CDS
GAAACGTGTGTCAACTCGAT+TGG 0.511678 contig304end:+4531 MsG0080048404.01.T01:intron
GCCTTTCTTCGTGATTCTTG+AGG 0.516103 contig304end:+4582 MsG0080048404.01.T01:CDS
TAGGTCCTCCAATCATCCAT+TGG 0.519762 contig304end:+4677 MsG0080048404.01.T01:intron
GGAAATTGGAGATAAATTAG+TGG 0.522367 contig304end:+4955 MsG0080048404.01.T01:three_prime_UTR
CCTCCTGTCATGACTCCTAA+AGG 0.532466 contig304end:-3992 None:intergenic
GTTGCAAGAAATCATTGTAA+AGG 0.533966 contig304end:-5076 None:intergenic
TCAGACTCCATCTCGAATAC+GGG 0.539823 contig304end:+3954 MsG0080048404.01.T01:CDS
CTCTTCTAGAGATTTACTCT+CGG 0.555374 contig304end:+4017 MsG0080048404.01.T01:CDS
AGGATATGGTTCATATCCAA+TGG 0.559473 contig304end:-4693 None:intergenic
GTTGTGTTGTCATTTATTGA+GGG 0.577748 contig304end:+4875 MsG0080048404.01.T01:three_prime_UTR
CTAGCTACCCGTATTCGAGA+TGG 0.580045 contig304end:-3961 None:intergenic
CATATCCAATGGATGATTGG+AGG 0.588211 contig304end:-4682 None:intergenic
TTCAGACAAAGGTGAAGGCA+AGG 0.592728 contig304end:+4720 MsG0080048404.01.T01:CDS
GAGAGTAAATCTCTAGAAGA+GGG 0.596002 contig304end:-4015 None:intergenic
GCTCCTTTAGGAGTCATGAC+AGG 0.609411 contig304end:+3989 MsG0080048404.01.T01:CDS
CGAGAGTAAATCTCTAGAAG+AGG 0.617070 contig304end:-4016 None:intergenic
ACTGGAACATCTATTGGTCA+TGG 0.617255 contig304end:+4420 MsG0080048404.01.T01:CDS
TGGATTTCAGACAAAGGTGA+AGG 0.621966 contig304end:+4715 MsG0080048404.01.T01:CDS
CGTATTCGAGATGGAGTCTG+AGG 0.625280 contig304end:-3952 None:intergenic
AAGACCTAGTGAAAATGCAA+TGG 0.638726 contig304end:+3929 MsG0080048404.01.T01:CDS
TTGTGTTGTCATTTATTGAG+GGG 0.653890 contig304end:+4876 MsG0080048404.01.T01:three_prime_UTR
CCTTTAGGAGTCATGACAGG+AGG 0.670470 contig304end:+3992 MsG0080048404.01.T01:CDS
TCTTGCTAGAGCAACTACAG+TGG 0.696223 contig304end:+5163 MsG0080048404.01.T01:three_prime_UTR
ATATCCTGGATTTCAGACAA+AGG 0.705635 contig304end:+4709 MsG0080048404.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATTTTTTTATTTCCAA+AGG - contig304end:4835-4854 None:intergenic 10.0%
!! AATAAAATTCTACAGAAGAT+TGG + contig304end:4848-4867 MsG0080048404.01.T01:three_prime_UTR 20.0%
!!! GACTATTTTAATTTGTTTGT+AGG + contig304end:4658-4677 MsG0080048404.01.T01:intron 20.0%
!!! TTTTTTTATTTCCAAAGGAA+TGG - contig304end:4830-4849 None:intergenic 20.0%
! ATTTGTGTTGTCTTTATTGA+GGG + contig304end:4933-4952 MsG0080048404.01.T01:intron 25.0%
! CAATTGTTGTGCTTTAATTA+TGG + contig304end:4352-4371 MsG0080048404.01.T01:intron 25.0%
! TACTTCAAAAATTCACAAGA+TGG - contig304end:5193-5212 None:intergenic 25.0%
! TTGAAATAGATAATCTTTGC+TGG + contig304end:5222-5241 MsG0080048404.01.T01:three_prime_UTR 25.0%
! TTGCTTTCAAAATGAAAACA+AGG + contig304end:4633-4652 MsG0080048404.01.T01:intron 25.0%
!! ATTATGGACTATAATCTTTC+AGG + contig304end:4368-4387 MsG0080048404.01.T01:intron 25.0%
!! TCGTTTTACTCACTTATATA+TGG - contig304end:4192-4211 None:intergenic 25.0%
ACAAAAGATGTTTATGACGA+AGG + contig304end:4298-4317 MsG0080048404.01.T01:intron 30.0%
AGCCAATATCAAAGAATTGA+AGG + contig304end:4748-4767 MsG0080048404.01.T01:CDS 30.0%
ATGTTAGGCTACATTCATTA+TGG + contig304end:4126-4145 MsG0080048404.01.T01:intron 30.0%
GATTTGTGTTGTCTTTATTG+AGG + contig304end:4932-4951 MsG0080048404.01.T01:intron 30.0%
GGAAATTGGAGATAAATTAG+TGG + contig304end:4955-4974 MsG0080048404.01.T01:three_prime_UTR 30.0%
GTCCTTCAATTCTTTGATAT+TGG - contig304end:4753-4772 None:intergenic 30.0%
GTTGCAAGAAATCATTGTAA+AGG - contig304end:5079-5098 None:intergenic 30.0%
TACTCACTTATATATGGATG+TGG - contig304end:4186-4205 None:intergenic 30.0%
TGTAACAGCTTAAATTGAGA+CGG - contig304end:4085-4104 None:intergenic 30.0%
TGTTAGGCTACATTCATTAT+GGG + contig304end:4127-4146 MsG0080048404.01.T01:intron 30.0%
TTAAATTGAGACGGACAAAT+TGG - contig304end:4076-4095 None:intergenic 30.0%
TTTGTGTTGTCTTTATTGAG+GGG + contig304end:4934-4953 MsG0080048404.01.T01:intron 30.0%
! GTTGTGTTGTCATTTATTGA+GGG + contig304end:4875-4894 MsG0080048404.01.T01:three_prime_UTR 30.0%
! TGTTGTGTTGTCATTTATTG+AGG + contig304end:4874-4893 MsG0080048404.01.T01:three_prime_UTR 30.0%
! TTGTGTTGTCATTTATTGAG+GGG + contig304end:4876-4895 MsG0080048404.01.T01:three_prime_UTR 30.0%
!! TATAATCTTTCAGGTTGTGT+TGG + contig304end:4377-4396 MsG0080048404.01.T01:intron 30.0%
AAGACCTAGTGAAAATGCAA+TGG + contig304end:3929-3948 MsG0080048404.01.T01:CDS 35.0%
AGGATATGGTTCATATCCAA+TGG - contig304end:4696-4715 None:intergenic 35.0%
ATATCCTGGATTTCAGACAA+AGG + contig304end:4709-4728 MsG0080048404.01.T01:CDS 35.0%
ATTGGATATGAACCATATCC+TGG + contig304end:4695-4714 MsG0080048404.01.T01:CDS 35.0%
CCATTAAAAAACCTTGAGCA+AGG - contig304end:4561-4580 None:intergenic 35.0%
CTACATTGACAACGAAATTG+AGG - contig304end:5051-5070 None:intergenic 35.0%
GCTGTTATAAACCATTCCTT+TGG + contig304end:4816-4835 MsG0080048404.01.T01:three_prime_UTR 35.0%
GTCATTTATTGAGGGGAAAT+TGG + contig304end:4883-4902 MsG0080048404.01.T01:intron 35.0%
GTTCATATCCAATGGATGAT+TGG - contig304end:4688-4707 None:intergenic 35.0%
TGTCTTTATTGAGGGGAAAT+TGG + contig304end:4941-4960 MsG0080048404.01.T01:intron 35.0%
! CTCTTCTAGAGATTTACTCT+CGG + contig304end:4017-4036 MsG0080048404.01.T01:CDS 35.0%
! GAGAGTAAATCTCTAGAAGA+GGG - contig304end:4018-4037 None:intergenic 35.0%
! TTTGTCTGAAATCCAGGATA+TGG - contig304end:4710-4729 None:intergenic 35.0%
!!! CCTTGCTCAAGGTTTTTTAA+TGG + contig304end:4558-4577 MsG0080048404.01.T01:CDS 35.0%
AAATTGGCAACAAGCTTGAC+AGG - contig304end:4060-4079 None:intergenic 40.0%
ACCTCAAGAATCACGAAGAA+AGG - contig304end:4586-4605 None:intergenic 40.0%
ACTGGAACATCTATTGGTCA+TGG + contig304end:4420-4439 MsG0080048404.01.T01:CDS 40.0%
CATATCCAATGGATGATTGG+AGG - contig304end:4685-4704 None:intergenic 40.0%
CTACATTCATTATGGGAGAG+GGG + contig304end:4134-4153 MsG0080048404.01.T01:intron 40.0%
GCTACATTCATTATGGGAGA+GGG + contig304end:4133-4152 MsG0080048404.01.T01:intron 40.0%
GTTTGTCATAGTGCTTGAAC+TGG + contig304end:4610-4629 MsG0080048404.01.T01:intron 40.0%
TCATGTGACGATGTGATGTT+AGG + contig304end:4111-4130 MsG0080048404.01.T01:intron 40.0%
TGGATTTCAGACAAAGGTGA+AGG + contig304end:4715-4734 MsG0080048404.01.T01:CDS 40.0%
! CGAGAGTAAATCTCTAGAAG+AGG - contig304end:4019-4038 None:intergenic 40.0%
! TTCACCTTTGTCTGAAATCC+AGG - contig304end:4716-4735 None:intergenic 40.0%
!! CAACACTGATTCTTCTCAAG+AGG + contig304end:5095-5114 MsG0080048404.01.T01:three_prime_UTR 40.0%
!! TGCTTTTCAGTGATGGAACA+AGG - contig304end:4462-4481 None:intergenic 40.0%
CTCAGACTCCATCTCGAATA+CGG + contig304end:3953-3972 MsG0080048404.01.T01:CDS 45.0%
GAAACGTGTGTCAACTCGAT+TGG + contig304end:4531-4550 MsG0080048404.01.T01:intron 45.0%
GCCTTTCTTCGTGATTCTTG+AGG + contig304end:4582-4601 MsG0080048404.01.T01:CDS 45.0%
GGCTACATTCATTATGGGAG+AGG + contig304end:4132-4151 MsG0080048404.01.T01:intron 45.0%
TAGCTAGACTCTGCTCCTTT+AGG + contig304end:3977-3996 MsG0080048404.01.T01:CDS 45.0%
TAGGTCCTCCAATCATCCAT+TGG + contig304end:4677-4696 MsG0080048404.01.T01:intron 45.0%
TATTGGTCATGGCGTGTTTG+AGG + contig304end:4431-4450 MsG0080048404.01.T01:CDS 45.0%
TCAGACTCCATCTCGAATAC+GGG + contig304end:3954-3973 MsG0080048404.01.T01:CDS 45.0%
TCTTGCTAGAGCAACTACAG+TGG + contig304end:5163-5182 MsG0080048404.01.T01:three_prime_UTR 45.0%
TTCAGACAAAGGTGAAGGCA+AGG + contig304end:4720-4739 MsG0080048404.01.T01:CDS 45.0%
! GAGGCCATTGCATTTTCACT+AGG - contig304end:3936-3955 None:intergenic 45.0%
! TCCATCACTGAAAAGCACGA+CGG + contig304end:4465-4484 MsG0080048404.01.T01:intron 45.0%
!! GGATGGACTGGAACATCTAT+TGG + contig304end:4414-4433 MsG0080048404.01.T01:CDS 45.0%
CCTCCTGTCATGACTCCTAA+AGG - contig304end:3995-4014 None:intergenic 50.0%
CGATTGGATGACCTTGCTCA+AGG + contig304end:4547-4566 MsG0080048404.01.T01:CDS 50.0%
CGTATTCGAGATGGAGTCTG+AGG - contig304end:3955-3974 None:intergenic 50.0%
CTAGCTACCCGTATTCGAGA+TGG - contig304end:3964-3983 None:intergenic 50.0%
GCAACAAGCTTGACAGGTGA+GGG - contig304end:4054-4073 None:intergenic 50.0%
GCTCAGTTGATTGGATGGAC+TGG + contig304end:4402-4421 MsG0080048404.01.T01:CDS 50.0%
TGGCTGCTCAGTTGATTGGA+TGG + contig304end:4397-4416 MsG0080048404.01.T01:CDS 50.0%
! CCTTTAGGAGTCATGACAGG+AGG + contig304end:3992-4011 MsG0080048404.01.T01:CDS 50.0%
! GCTCCTTTAGGAGTCATGAC+AGG + contig304end:3989-4008 MsG0080048404.01.T01:CDS 50.0%
! TCATTATGGGAGAGGGGTAC+TGG + contig304end:4140-4159 MsG0080048404.01.T01:intron 50.0%
! TCCGTCGTGCTTTTCAGTGA+TGG - contig304end:4469-4488 None:intergenic 50.0%
!! CATTATGGGAGAGGGGTACT+GGG + contig304end:4141-4160 MsG0080048404.01.T01:intron 50.0%
!! GTGTTGGCTGCTCAGTTGAT+TGG + contig304end:4393-4412 MsG0080048404.01.T01:CDS 50.0%
GGCAACAAGCTTGACAGGTG+AGG - contig304end:4055-4074 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
contig304end gene 3922 5246 3922 ID=MsG0080048404.01;Name=MsG0080048404.01
contig304end mRNA 3922 5246 3922 ID=MsG0080048404.01.T01;Parent=MsG0080048404.01;Name=MsG0080048404.01.T01;_AED=0.49;_eAED=0.57;_QI=0|0.75|0.8|1|0.75|0.6|5|392|122
contig304end exon 3922 4038 3922 ID=MsG0080048404.01.T01:exon:10573;Parent=MsG0080048404.01.T01
contig304end exon 4390 4452 4390 ID=MsG0080048404.01.T01:exon:10574;Parent=MsG0080048404.01.T01
contig304end exon 4532 4603 4532 ID=MsG0080048404.01.T01:exon:10575;Parent=MsG0080048404.01.T01
contig304end exon 4680 4895 4680 ID=MsG0080048404.01.T01:exon:10576;Parent=MsG0080048404.01.T01
contig304end exon 4954 5246 4954 ID=MsG0080048404.01.T01:exon:10577;Parent=MsG0080048404.01.T01
contig304end CDS 3922 4038 3922 ID=MsG0080048404.01.T01:cds;Parent=MsG0080048404.01.T01
contig304end CDS 4390 4452 4390 ID=MsG0080048404.01.T01:cds;Parent=MsG0080048404.01.T01
contig304end CDS 4532 4603 4532 ID=MsG0080048404.01.T01:cds;Parent=MsG0080048404.01.T01
contig304end CDS 4680 4796 4680 ID=MsG0080048404.01.T01:cds;Parent=MsG0080048404.01.T01
contig304end three_prime_UTR 4797 4895 4797 ID=MsG0080048404.01.T01:three_prime_utr;Parent=MsG0080048404.01.T01
contig304end three_prime_UTR 4954 5246 4954 ID=MsG0080048404.01.T01:three_prime_utr;Parent=MsG0080048404.01.T01
Gene Sequence

>MsG0080048404.01.T01

ATGAGGCAAGACCTAGTGAAAATGCAATGGCCTCAGACTCCATCTCGAATACGGGTAGCTAGACTCTGCTCCTTTAGGAGTCATGACAGGAGGCCCTCTTCTAGAGATTTACTCTCGGTTGTGTTGGCTGCTCAGTTGATTGGATGGACTGGAACATCTATTGGTCATGGCGTGTTTGAGAAACGTGTGTCAACTCGATTGGATGACCTTGCTCAAGGTTTTTTAATGGAGCCTTTCTTCGTGATTCTTGAGGTCCTCCAATCATCCATTGGATATGAACCATATCCTGGATTTCAGACAAAGGTGAAGGCAAGGATAGAAGCCAATATCAAAGAATTGAAGGACAGTGAACAAAAGAAACTTTCTTAG

Protein sequence

>MsG0080048404.01.T01

MRQDLVKMQWPQTPSRIRVARLCSFRSHDRRPSSRDLLSVVLAAQLIGWTGTSIGHGVFEKRVSTRLDDLAQGFLMEPFFVILEVLQSSIGYEPYPGFQTKVKARIEANIKELKDSEQKKLS*