AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280008118.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008118.01.T01 MTR_2g035930 100.000 135 0 0 1 135 1 135 5.91e-95 270
MsG0280008118.01.T01 MTR_2g006170 99.259 135 1 0 1 135 1 135 2.02e-94 268
MsG0280008118.01.T01 MTR_3g048580 64.179 67 15 2 70 135 38 96 3.04e-19 77.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280008118.01.T01 AT4G15000 86.667 135 18 0 1 135 1 135 7.88e-84 241
MsG0280008118.01.T01 AT3G22230 86.667 135 18 0 1 135 1 135 1.24e-83 241
MsG0280008118.01.T01 AT2G32220 80.000 135 27 0 1 135 1 135 7.77e-78 226
MsG0280008118.01.T01 AT4G15000 85.366 123 18 0 1 123 1 123 4.27e-73 214

Find 34 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACGATCACGGCTTTCTTCC+CGG 0.266318 2:-25656498 None:intergenic
TACGGGCATTGTCTTGTTGC+AGG 0.269826 2:+25656554 MsG0280008118.01.T01:CDS
GGTTTAAGACTGGGAAGAAT+AGG 0.401208 2:+25656801 MsG0280008118.01.T01:CDS
GGAACTCGCGACCGTGCTTA+CGG 0.402067 2:+25656536 MsG0280008118.01.T01:CDS
TACTGATGTTCTTCAGAGTA+AGG 0.417538 2:+25656730 MsG0280008118.01.T01:CDS
AGAACGATGACGGCTTTGTT+TGG 0.417683 2:-25656461 None:intergenic
TAAGAAGGTGACTGCTTTGA+AGG 0.432710 2:+25656754 MsG0280008118.01.T01:CDS
TCCTTCTTGATAACCTTAGC+TGG 0.439811 2:-25656590 None:intergenic
TTCTTCAAGGACGCTACGCC+GGG 0.449272 2:+25656480 MsG0280008118.01.T01:CDS
CTTCACACGCGATTTCTTCG+CGG 0.451730 2:-25656627 None:intergenic
GAACTCGCGACCGTGCTTAC+GGG 0.459112 2:+25656537 MsG0280008118.01.T01:CDS
GGTGGTTCTTTACCAAGCTC+AGG 0.462687 2:+25656822 MsG0280008118.01.T01:CDS
AAAGCCGTCATCGTTCTTCA+AGG 0.467401 2:+25656467 MsG0280008118.01.T01:CDS
GTCGAAATTCTTCACGATCA+CGG 0.498548 2:-25656510 None:intergenic
TTCGAGGAGAGGTTTAAGAC+TGG 0.506310 2:+25656791 MsG0280008118.01.T01:CDS
ACGGGCATTGTCTTGTTGCA+GGG 0.511441 2:+25656555 MsG0280008118.01.T01:CDS
CGCGAAGAAATCGCGTGTGA+AGG 0.529778 2:+25656628 MsG0280008118.01.T01:CDS
AACGAGTCGGCATCAGATGC+TGG 0.530504 2:-25656673 None:intergenic
CGAAGAAGAGGTTCGAGGAG+AGG 0.532977 2:+25656780 MsG0280008118.01.T01:CDS
TCCAGCTAAGGTTATCAAGA+AGG 0.538513 2:+25656589 MsG0280008118.01.T01:CDS
TCGAGGAGAGGTTTAAGACT+GGG 0.540939 2:+25656792 MsG0280008118.01.T01:CDS
AACAGCTCAAAACCTGAGCT+TGG 0.543643 2:-25656834 None:intergenic
TCTTGATGTTGATTTGAAAG+AGG 0.559046 2:+25656700 MsG0280008118.01.T01:CDS
TCTTCAGAGTAAGGATAAGA+AGG 0.568239 2:+25656739 MsG0280008118.01.T01:CDS
GTTCTTCAAGGACGCTACGC+CGG 0.577838 2:+25656479 MsG0280008118.01.T01:CDS
CAAGACAATGCCCGTAAGCA+CGG 0.587108 2:-25656547 None:intergenic
CTTTGAAGGAGGCGAAGAAG+AGG 0.602084 2:+25656768 MsG0280008118.01.T01:CDS
GATCAAGAAGTATCCAGCTA+AGG 0.602579 2:+25656577 MsG0280008118.01.T01:CDS
GGAGGCGAAGAAGAGGTTCG+AGG 0.607573 2:+25656775 MsG0280008118.01.T01:CDS
TTAAGACTGGGAAGAATAGG+TGG 0.614047 2:+25656804 MsG0280008118.01.T01:CDS
GAAGGTGACTGCTTTGAAGG+AGG 0.640915 2:+25656757 MsG0280008118.01.T01:CDS
GCGTCCTTGAAGAACGATGA+CGG 0.645066 2:-25656471 None:intergenic
ACATCAAGAGTGTAACGAGT+CGG 0.653533 2:-25656686 None:intergenic
ATCGTGAAGAATTTCGACGA+CGG 0.719572 2:+25656515 MsG0280008118.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTGATGTTGATTTGAAAG+AGG + Chr2:25656700-25656719 MsG0280008118.01.T01:CDS 30.0%
TACTGATGTTCTTCAGAGTA+AGG + Chr2:25656730-25656749 MsG0280008118.01.T01:CDS 35.0%
TCTTCAGAGTAAGGATAAGA+AGG + Chr2:25656739-25656758 MsG0280008118.01.T01:CDS 35.0%
ATCGTGAAGAATTTCGACGA+CGG + Chr2:25656515-25656534 MsG0280008118.01.T01:CDS 40.0%
GTCGAAATTCTTCACGATCA+CGG - Chr2:25656513-25656532 None:intergenic 40.0%
TAAGAAGGTGACTGCTTTGA+AGG + Chr2:25656754-25656773 MsG0280008118.01.T01:CDS 40.0%
TCCAGCTAAGGTTATCAAGA+AGG + Chr2:25656589-25656608 MsG0280008118.01.T01:CDS 40.0%
TTAAGACTGGGAAGAATAGG+TGG + Chr2:25656804-25656823 MsG0280008118.01.T01:CDS 40.0%
! ACATCAAGAGTGTAACGAGT+CGG - Chr2:25656689-25656708 None:intergenic 40.0%
! GGTTTAAGACTGGGAAGAAT+AGG + Chr2:25656801-25656820 MsG0280008118.01.T01:CDS 40.0%
! TCCTTCTTGATAACCTTAGC+TGG - Chr2:25656593-25656612 None:intergenic 40.0%
!! GATCAAGAAGTATCCAGCTA+AGG + Chr2:25656577-25656596 MsG0280008118.01.T01:CDS 40.0%
AAAGCCGTCATCGTTCTTCA+AGG + Chr2:25656467-25656486 MsG0280008118.01.T01:CDS 45.0%
! TCGAGGAGAGGTTTAAGACT+GGG + Chr2:25656792-25656811 MsG0280008118.01.T01:CDS 45.0%
! TTCGAGGAGAGGTTTAAGAC+TGG + Chr2:25656791-25656810 MsG0280008118.01.T01:CDS 45.0%
!! AGAACGATGACGGCTTTGTT+TGG - Chr2:25656464-25656483 None:intergenic 45.0%
CAAGACAATGCCCGTAAGCA+CGG - Chr2:25656550-25656569 None:intergenic 50.0%
CTTCACACGCGATTTCTTCG+CGG - Chr2:25656630-25656649 None:intergenic 50.0%
GCGTCCTTGAAGAACGATGA+CGG - Chr2:25656474-25656493 None:intergenic 50.0%
GGTGGTTCTTTACCAAGCTC+AGG + Chr2:25656822-25656841 MsG0280008118.01.T01:CDS 50.0%
! ACGGGCATTGTCTTGTTGCA+GGG + Chr2:25656555-25656574 MsG0280008118.01.T01:CDS 50.0%
! CTTTGAAGGAGGCGAAGAAG+AGG + Chr2:25656768-25656787 MsG0280008118.01.T01:CDS 50.0%
! TACGGGCATTGTCTTGTTGC+AGG + Chr2:25656554-25656573 MsG0280008118.01.T01:CDS 50.0%
!! GAAGGTGACTGCTTTGAAGG+AGG + Chr2:25656757-25656776 MsG0280008118.01.T01:CDS 50.0%
AACGAGTCGGCATCAGATGC+TGG - Chr2:25656676-25656695 None:intergenic 55.0%
CACGATCACGGCTTTCTTCC+CGG - Chr2:25656501-25656520 None:intergenic 55.0%
CGAAGAAGAGGTTCGAGGAG+AGG + Chr2:25656780-25656799 MsG0280008118.01.T01:CDS 55.0%
CGCGAAGAAATCGCGTGTGA+AGG + Chr2:25656628-25656647 MsG0280008118.01.T01:CDS 55.0%
GTTCTTCAAGGACGCTACGC+CGG + Chr2:25656479-25656498 MsG0280008118.01.T01:CDS 55.0%
TTCTTCAAGGACGCTACGCC+GGG + Chr2:25656480-25656499 MsG0280008118.01.T01:CDS 55.0%
GAACTCGCGACCGTGCTTAC+GGG + Chr2:25656537-25656556 MsG0280008118.01.T01:CDS 60.0%
GGAACTCGCGACCGTGCTTA+CGG + Chr2:25656536-25656555 MsG0280008118.01.T01:CDS 60.0%
GGAGGCGAAGAAGAGGTTCG+AGG + Chr2:25656775-25656794 MsG0280008118.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 25656443 25656850 25656443 ID=MsG0280008118.01;Name=MsG0280008118.01
Chr2 mRNA 25656443 25656850 25656443 ID=MsG0280008118.01.T01;Parent=MsG0280008118.01;Name=MsG0280008118.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|135
Chr2 exon 25656443 25656850 25656443 ID=MsG0280008118.01.T01:exon:34949;Parent=MsG0280008118.01.T01
Chr2 CDS 25656443 25656850 25656443 ID=MsG0280008118.01.T01:cds;Parent=MsG0280008118.01.T01
Gene Sequence

>MsG0280008118.01.T01

ATGGTGAAATTCTTGAAACCAAACAAAGCCGTCATCGTTCTTCAAGGACGCTACGCCGGGAAGAAAGCCGTGATCGTGAAGAATTTCGACGACGGAACTCGCGACCGTGCTTACGGGCATTGTCTTGTTGCAGGGATCAAGAAGTATCCAGCTAAGGTTATCAAGAAGGATTCAGCAAAGAAAACCGCGAAGAAATCGCGTGTGAAGGCTTTCGTGAAGCTTGTTAACTACCAGCATCTGATGCCGACTCGTTACACTCTTGATGTTGATTTGAAAGAGGTTGTTACTACTGATGTTCTTCAGAGTAAGGATAAGAAGGTGACTGCTTTGAAGGAGGCGAAGAAGAGGTTCGAGGAGAGGTTTAAGACTGGGAAGAATAGGTGGTTCTTTACCAAGCTCAGGTTTTGA

Protein sequence

>MsG0280008118.01.T01

MVKFLKPNKAVIVLQGRYAGKKAVIVKNFDDGTRDRAYGHCLVAGIKKYPAKVIKKDSAKKTAKKSRVKAFVKLVNYQHLMPTRYTLDVDLKEVVTTDVLQSKDKKVTALKEAKKRFEERFKTGKNRWFFTKLRF*