AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041091.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041091.01.T01 MTR_7g104080 84.783 92 2 1 6 97 719 798 3.10e-48 164
MsG0780041091.01.T01 MTR_7g104080 84.783 92 2 1 6 97 722 801 3.31e-48 164
MsG0780041091.01.T01 MTR_3g115340 45.556 90 37 1 7 96 636 713 1.40e-19 83.2
MsG0780041091.01.T01 MTR_3g115340 45.556 90 37 1 7 96 649 726 1.43e-19 83.2
MsG0780041091.01.T01 MTR_3g115340 45.556 90 37 1 7 96 565 642 1.95e-19 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041091.01.T01 AT2G36390 71.739 92 14 1 6 97 709 788 1.67e-41 145
MsG0780041091.01.T01 AT5G03650 66.304 92 19 1 6 97 674 753 3.97e-37 133

Find 22 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACACCATATCTTTCTTCT+AGG 0.172344 7:+86444828 None:intergenic
ATCATCTATGTTTCTTAATC+AGG 0.216907 7:-86444909 None:intergenic
CTGAGCACCAATATATTTCA+AGG 0.360464 7:-86444459 MsG0780041091.01.T01:CDS
TTTAAAGCCAGGGAAATATA+AGG 0.403419 7:-86444335 MsG0780041091.01.T01:CDS
AAATTAAGTGTGCCTACCTT+TGG 0.418326 7:-86444497 MsG0780041091.01.T01:intron
AGTTATTCAGATTACAAAGT+TGG 0.437881 7:-86444361 MsG0780041091.01.T01:CDS
GATAGTACCTTATATTTCCC+TGG 0.449188 7:+86444328 None:intergenic
GCAGAATATCTAAGATATCA+TGG 0.471184 7:-86444881 MsG0780041091.01.T01:CDS
AAAGTTGGCTGTTTAAAGCC+AGG 0.472552 7:-86444346 MsG0780041091.01.T01:CDS
CAGCACCTAGAAGAAAGATA+TGG 0.490578 7:-86444833 MsG0780041091.01.T01:CDS
ATAGCTTTGCATAATTGTAA+AGG 0.491289 7:+86444260 None:intergenic
TTTGTAATCTGAATAACTGT+TGG 0.514908 7:+86444366 None:intergenic
CTATCTATGTATTATTACTC+TGG 0.518437 7:-86444310 MsG0780041091.01.T01:CDS
TCATCTATGTTTCTTAATCA+GGG 0.524782 7:-86444908 None:intergenic
AATCTTCGAAAGGGACAACC+TGG 0.550488 7:-86444413 MsG0780041091.01.T01:CDS
AAGTTGGCTGTTTAAAGCCA+GGG 0.561656 7:-86444345 MsG0780041091.01.T01:CDS
GAAATCATGAACTGCACCAA+AGG 0.571081 7:+86444481 None:intergenic
CAGAGTTATAATCTTCGAAA+GGG 0.572676 7:-86444422 MsG0780041091.01.T01:CDS
ACAGAGTTATAATCTTCGAA+AGG 0.593777 7:-86444423 MsG0780041091.01.T01:CDS
TTGCAGATAGTATTCAACCT+AGG 0.598602 7:-86444587 MsG0780041091.01.T01:intron
TCATGAACTGCACCAAAGGT+AGG 0.636371 7:+86444485 None:intergenic
GAAAATTGAATACAAAGACC+AGG 0.647072 7:+86444395 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTTCAAGGAAAAATGA+AGG - Chr7:86444712-86444731 MsG0780041091.01.T01:intron 20.0%
!!! CATTTTTCCTTGAAATATAT+TGG + Chr7:86444711-86444730 None:intergenic 20.0%
!!! TAGGGGTTAATATTATTTTT+TGG - Chr7:86444615-86444634 MsG0780041091.01.T01:intron 20.0%
!!! TTTTAATAACTAAGCAGTTA+AGG - Chr7:86444639-86444658 MsG0780041091.01.T01:intron 20.0%
! AGTTATTCAGATTACAAAGT+TGG - Chr7:86444799-86444818 MsG0780041091.01.T01:intron 25.0%
! CTATCTATGTATTATTACTC+TGG - Chr7:86444850-86444869 MsG0780041091.01.T01:CDS 25.0%
! TTTGTAATCTGAATAACTGT+TGG + Chr7:86444797-86444816 None:intergenic 25.0%
!! ATAGCTTTGCATAATTGTAA+AGG + Chr7:86444903-86444922 None:intergenic 25.0%
!! TCTTTGTATTCAATTTTCAC+TGG - Chr7:86444770-86444789 MsG0780041091.01.T01:intron 25.0%
!! TTGATTTAGAGATAACTTGT+AGG - Chr7:86444435-86444454 MsG0780041091.01.T01:CDS 25.0%
!! TTTTTCTCACAAATGAGATA+GGG - Chr7:86444597-86444616 MsG0780041091.01.T01:intron 25.0%
!!! TTTTTTCTCACAAATGAGAT+AGG - Chr7:86444596-86444615 MsG0780041091.01.T01:intron 25.0%
ACAGAGTTATAATCTTCGAA+AGG - Chr7:86444737-86444756 MsG0780041091.01.T01:intron 30.0%
CAGAGTTATAATCTTCGAAA+GGG - Chr7:86444738-86444757 MsG0780041091.01.T01:intron 30.0%
GAAAATTGAATACAAAGACC+AGG + Chr7:86444768-86444787 None:intergenic 30.0%
TCATTTGTGAGAAAAAACCT+AGG + Chr7:86444593-86444612 None:intergenic 30.0%
TTACACCATATCTTTCTTCT+AGG + Chr7:86444335-86444354 None:intergenic 30.0%
! GCAGAATATCTAAGATATCA+TGG - Chr7:86444279-86444298 MsG0780041091.01.T01:CDS 30.0%
! TTTAAAGCCAGGGAAATATA+AGG - Chr7:86444825-86444844 MsG0780041091.01.T01:intron 30.0%
! TTTTCTCACAAATGAGATAG+GGG - Chr7:86444598-86444617 MsG0780041091.01.T01:intron 30.0%
AAATTAAGTGTGCCTACCTT+TGG - Chr7:86444663-86444682 MsG0780041091.01.T01:intron 35.0%
ACTTCAAATGACAAGCAGAT+AGG + Chr7:86444402-86444421 None:intergenic 35.0%
CTGAGCACCAATATATTTCA+AGG - Chr7:86444701-86444720 MsG0780041091.01.T01:intron 35.0%
GATAGTACCTTATATTTCCC+TGG + Chr7:86444835-86444854 None:intergenic 35.0%
TTGCAGATAGTATTCAACCT+AGG - Chr7:86444573-86444592 MsG0780041091.01.T01:intron 35.0%
! AGAGATAACTTGTAGGAAGT+TGG - Chr7:86444442-86444461 MsG0780041091.01.T01:CDS 35.0%
CAGCACCTAGAAGAAAGATA+TGG - Chr7:86444327-86444346 MsG0780041091.01.T01:CDS 40.0%
GAAATCATGAACTGCACCAA+AGG + Chr7:86444682-86444701 None:intergenic 40.0%
! AAAGTTGGCTGTTTAAAGCC+AGG - Chr7:86444814-86444833 MsG0780041091.01.T01:intron 40.0%
! AAGTTGGCTGTTTAAAGCCA+GGG - Chr7:86444815-86444834 MsG0780041091.01.T01:intron 40.0%
AATCTTCGAAAGGGACAACC+TGG - Chr7:86444747-86444766 MsG0780041091.01.T01:intron 45.0%
TCATGAACTGCACCAAAGGT+AGG + Chr7:86444678-86444697 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr7 gene 86444258 86444924 86444258 ID=MsG0780041091.01;Name=MsG0780041091.01
Chr7 mRNA 86444258 86444924 86444258 ID=MsG0780041091.01.T01;Parent=MsG0780041091.01;Name=MsG0780041091.01.T01;_AED=0.50;_eAED=0.63;_QI=0|0|0|1|0|0.33|3|0|120
Chr7 exon 86444832 86444924 86444832 ID=MsG0780041091.01.T01:exon:3136;Parent=MsG0780041091.01.T01
Chr7 exon 86444588 86444603 86444588 ID=MsG0780041091.01.T01:exon:3135;Parent=MsG0780041091.01.T01
Chr7 exon 86444258 86444511 86444258 ID=MsG0780041091.01.T01:exon:3134;Parent=MsG0780041091.01.T01
Chr7 CDS 86444832 86444924 86444832 ID=MsG0780041091.01.T01:cds;Parent=MsG0780041091.01.T01
Chr7 CDS 86444588 86444603 86444588 ID=MsG0780041091.01.T01:cds;Parent=MsG0780041091.01.T01
Chr7 CDS 86444258 86444511 86444258 ID=MsG0780041091.01.T01:cds;Parent=MsG0780041091.01.T01
Gene Sequence

>MsG0780041091.01.T01

ATGTTTCTTAATCAGGGTGATGCAGAATATCTAAGATATCATGGAATGCAAGAATTTGATCGAGCTATGCAGCACCTAGAAGAAAGATATGGTATAGTATTCAACCTAGTGTGCCTACCTTTGGTGCAGTTCATGATTTCTGAGCACCAATATATTTCAAGGAAAAATGAAGGCGACAGAGTTATAATCTTCGAAAGGGACAACCTGGTCTTTGTATTCAATTTTCACTGGACCAACAGTTATTCAGATTACAAAGTTGGCTGTTTAAAGCCAGGGAAATATAAGGTACTATCTATGTATTATTACTCTGGCAAGACTAAAGTAGTAACAATAAAAGTCCTTTACAATTATGCAAAGCTATGA

Protein sequence

>MsG0780041091.01.T01

MFLNQGDAEYLRYHGMQEFDRAMQHLEERYGIVFNLVCLPLVQFMISEHQYISRKNEGDRVIIFERDNLVFVFNFHWTNSYSDYKVGCLKPGKYKVLSMYYYSGKTKVVTIKVLYNYAKL*