AlfalfaGEDB | MsG0580025592.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025592.01

KEGG: -
GO: GO:0030246
PFAM: PF00139

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025592.01.T01	XP_003612969.1	92.276	246	19	0	2	247	41	286	3.73E-164	465

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025592.01.T01	Q2PP74	77.778	243	53	1	6	247	37	279	1.01E-138	393

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025592.01.T01	A9YWS6	92.276	246	19	0	2	247	41	286	1.78e-164	465

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0180004091.01	MsG0580025592.01	PPI
MsG0180004091.01	MsG0580025592.01	PPI
MsG0180004091.01	MsG0580025592.01	PPI
MsG0180004091.01	MsG0580025592.01	PPI
MsG0180005059.01	MsG0580025592.01	PPI
MsG0180005060.01	MsG0580025592.01	PPI
MsG0180005082.01	MsG0580025592.01	PPI
MsG0380013594.01	MsG0580025592.01	PPI
MsG0380013595.01	MsG0580025592.01	PPI
MsG0380014272.01	MsG0580025592.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025592.01.T01	MTR_5g031140	92.276	246	19	0	2	247	41	286	4.52e-168	465
MsG0580025592.01.T01	MTR_5g031120	77.366	243	54	1	6	247	37	279	1.42e-139	393
MsG0580025592.01.T01	MTR_5g031100	75.309	243	58	2	6	247	37	278	8.88e-134	378
MsG0580025592.01.T01	MTR_5g031160	74.089	247	63	1	2	247	41	287	4.08e-131	372
MsG0580025592.01.T01	MTR_5g031030	50.820	244	114	3	6	247	35	274	1.22e-76	233
MsG0580025592.01.T01	MTR_5g031090	48.361	244	124	2	6	247	32	275	1.59e-74	228
MsG0580025592.01.T01	MTR_8g068040	44.444	252	124	5	1	247	21	261	1.55e-65	204
MsG0580025592.01.T01	MTR_8g068030	43.750	256	124	5	1	247	21	265	1.92e-63	199
MsG0580025592.01.T01	MTR_8g068050	43.496	246	131	4	4	247	34	273	2.23e-62	207
MsG0580025592.01.T01	MTR_8g067980	47.639	233	107	9	18	246	37	258	5.60e-56	180

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025592.01.T01	AT5G10530	32.520	246	151	8	7	247	19	254	2.60e-31	121
MsG0580025592.01.T01	AT5G65600	31.933	238	146	8	6	236	34	262	1.61e-26	108
MsG0580025592.01.T01	AT5G55830	32.806	253	144	12	6	246	29	267	8.28e-20	88.6
MsG0580025592.01.T01	AT4G29050	31.579	228	129	10	31	244	59	273	3.06e-18	84.0
MsG0580025592.01.T01	AT4G29050	31.579	228	129	10	31	244	45	259	3.25e-18	84.0
MsG0580025592.01.T01	AT3G53810	30.859	256	144	12	6	244	23	262	1.01e-17	82.4
MsG0580025592.01.T01	AT2G37710	28.750	240	144	12	19	243	34	261	2.45e-15	75.5
MsG0580025592.01.T01	AT4G02420	30.651	261	144	10	1	243	19	260	2.61e-15	75.5
MsG0580025592.01.T01	AT5G06740	26.496	234	160	7	15	244	32	257	1.42e-14	73.2
MsG0580025592.01.T01	AT5G06740	26.496	234	160	7	15	244	44	269	1.51e-14	73.2

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Find 50 sgRNAs with CRISPR-Local

Find 67 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCCCGACATATTTCTC+AGG	0.449696	5:+20971707	MsG0580025592.01.T01:CDS
AAATACATGATATCATTAAT+TGG	0.342487	5:+20972355	MsG0580025592.01.T01:CDS
AAATCCCGACATATTTCTCA+GGG	0.568484	5:+20971708	MsG0580025592.01.T01:CDS
AAGGATCAAATGCTGTCTCC+GGG	0.572192	5:-20972005	None:intergenic
AATCCAACTCTATCCCATTC+AGG	0.383760	5:-20972306	None:intergenic
AATCCCGACATATTTCTCAG+GGG	0.779489	5:+20971709	MsG0580025592.01.T01:CDS
AATTCAAGTGGCGGCTATCT+TGG	0.364845	5:+20971973	MsG0580025592.01.T01:CDS
ACAAACGGTTCAGAATTCCA+CGG	0.693197	5:+20971901	MsG0580025592.01.T01:CDS
AGAAAGCAAATCCATCTCCG+TGG	0.678902	5:-20971918	None:intergenic
AGCTTCTCCTAAAGCATTTC+TGG	0.122535	5:-20972172	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATCTCACACCAATTCATATT+TGG	+	Chr5:20971821-20971840	MsG0580025592.01.T01:CDS	30.0%
GTGAAAGAAACAGTATTTCT+TGG	-	Chr5:20971667-20971686	None:intergenic	30.0%
TCGAACTCAATAGCTATTAT+TGG	-	Chr5:20972039-20972058	None:intergenic	30.0%
TCTCACACCAATTCATATTT+GGG	+	Chr5:20971822-20971841	MsG0580025592.01.T01:CDS	30.0%
AAAATCCCGACATATTTCTC+AGG	+	Chr5:20971707-20971726	MsG0580025592.01.T01:CDS	35.0%
AAATCCCGACATATTTCTCA+GGG	+	Chr5:20971708-20971727	MsG0580025592.01.T01:CDS	35.0%
CACGAAAAAGAAGAAATGAG+TGG	-	Chr5:20971878-20971897	None:intergenic	35.0%
CCAATATCAATTCCAATGTG+AGG	-	Chr5:20972105-20972124	None:intergenic	35.0%
CCTCACATTGGAATTGATAT+TGG	+	Chr5:20972102-20972121	MsG0580025592.01.T01:CDS	35.0%
CTAACAAAAACCAACCAATC+CGG	+	Chr5:20971763-20971782	MsG0580025592.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	20971655	20972398	20971655	ID=MsG0580025592.01;Name=MsG0580025592.01
Chr5	mRNA	20971655	20972398	20971655	ID=MsG0580025592.01.T01;Parent=MsG0580025592.01;Name=MsG0580025592.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|247
Chr5	exon	20971655	20972398	20971655	ID=MsG0580025592.01.T01:exon:23440;Parent=MsG0580025592.01.T01
Chr5	CDS	20971655	20972398	20971655	ID=MsG0580025592.01.T01:cds;Parent=MsG0580025592.01.T01

Gene Sequence

>MsG0580025592.01.T01

TTGCAACAACCAAGAAATACTGTTTCTTTCACTATATTAGAATTCGAGAAAGAAAATCCCGACATATTTCTCAGGGGAGATGCCTCAATCTCCGGTGGCATTTTAAAGCTAACAAAAACCAACCAATCCGGTGAACCACTCCAAAAGAGCGTTGGACGAGCAACACATCTCACACCAATTCATATTTGGGATAAAACTAGTGGAAAACTAGCAGATTTCTCCACTCATTTCTTCTTTTTCGTGAACACAAACGGTTCAGAATTCCACGGAGATGGATTTGCTTTCTATATCGGACCACTTCATTTTGAAGTACCGAAAAATTCAAGTGGCGGCTATCTTGGTCTTTTCGACCCGGAGACAGCATTTGATCCTTCCAAAAATCCAATAATAGCTATTGAGTTCGATAGTTTTACAAATGGGTGGGATCCAGCTTCACCATCTCAATATCCTCACATTGGAATTGATATTGGTTCTATTGATTCAGTTGCAACTGTGAACTGGCATGTTGATGTTTTGTCCAGAAATGCTTTAGGAGAAGCTATTATAAACTATAACTCCGAGTCTAAGAGATTGAGTGTGTTTGTGGATTATCCTGGGAGCGGGACGAAAGCAACTGGTATCTCTTTTGTTGTTGATTTGAGGAGTATTCTTCCTGAATGGGATAGAGTTGGATTCTCGGCTGCAGCAGGAGAGTTGGTTGAAATACATGATATCATTAATTGGTCTTTCGAATCATCTTTGTAA

Protein sequence

>MsG0580025592.01.T01

LQQPRNTVSFTILEFEKENPDIFLRGDASISGGILKLTKTNQSGEPLQKSVGRATHLTPIHIWDKTSGKLADFSTHFFFFVNTNGSEFHGDGFAFYIGPLHFEVPKNSSGGYLGLFDPETAFDPSKNPIIAIEFDSFTNGWDPASPSQYPHIGIDIGSIDSVATVNWHVDVLSRNALGEAIINYNSESKRLSVFVDYPGSGTKATGISFVVDLRSILPEWDRVGFSAAAGELVEIHDIINWSFESSL*