Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025769.01.T01 | XP_003613226.1 | 95.968 | 248 | 10 | 0 | 1 | 248 | 1 | 248 | 1.62E-137 | 396 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048177.01 | MsG0580025769.01 | 0.829951 | 3.860756e-55 | 4.010789e-52 |
| MsG0080048178.01 | MsG0580025769.01 | 0.819635 | 1.047251e-52 | 8.103208e-50 |
| MsG0080048210.01 | MsG0580025769.01 | 0.810602 | 1.062598e-50 | 6.433183e-48 |
| MsG0080048656.01 | MsG0580025769.01 | 0.817878 | 2.623574e-52 | 1.933850e-49 |
| MsG0180000157.01 | MsG0580025769.01 | 0.865405 | 6.050028e-65 | 1.966018e-61 |
| MsG0180000197.01 | MsG0580025769.01 | 0.858748 | 6.657251e-63 | 1.716861e-59 |
| MsG0180000222.01 | MsG0580025769.01 | 0.811411 | 7.094797e-51 | 4.388732e-48 |
| MsG0180000235.01 | MsG0580025769.01 | 0.803327 | 3.675268e-49 | 1.841604e-46 |
| MsG0180000555.01 | MsG0580025769.01 | 0.804445 | 2.152936e-49 | 1.110238e-46 |
| MsG0180000585.01 | MsG0580025769.01 | 0.829799 | 4.204892e-55 | 4.349886e-52 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025769.01.T01 | MTR_5g034230 | 95.968 | 248 | 10 | 0 | 1 | 248 | 1 | 248 | 1.96e-141 | 396 |
| MsG0580025769.01.T01 | MTR_8g064650 | 39.111 | 225 | 118 | 7 | 36 | 248 | 103 | 320 | 2.67e-36 | 131 |
| MsG0580025769.01.T01 | MTR_3g077890 | 30.488 | 246 | 112 | 9 | 32 | 248 | 70 | 285 | 4.10e-21 | 90.1 |
| MsG0580025769.01.T01 | MTR_4g105750 | 30.256 | 195 | 106 | 9 | 9 | 190 | 25 | 202 | 1.39e-14 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025769.01.T01 | AT1G70420 | 42.146 | 261 | 123 | 7 | 9 | 248 | 19 | 272 | 2.63e-48 | 160 |
| MsG0580025769.01.T01 | AT1G23710 | 36.054 | 294 | 133 | 10 | 1 | 248 | 11 | 295 | 1.41e-39 | 139 |
| MsG0580025769.01.T01 | AT3G27880 | 35.075 | 134 | 59 | 3 | 124 | 240 | 114 | 236 | 7.38e-13 | 66.6 |
Find 48 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTTCTTTACCGGCGAA+CGG | 0.570175 | 5:-23914124 | None:intergenic |
| AAAGGCGGCTCCGGCGCCGT+CGG | 0.495359 | 5:+23914415 | MsG0580025769.01.T01:CDS |
| AACACATCGTCAGCGGAAAT+CGG | 0.392748 | 5:-23914017 | None:intergenic |
| AAGAAAGTGAAAATGGTGAA+AGG | 0.583207 | 5:+23914389 | MsG0580025769.01.T01:CDS |
| AAGGTAGAGAAAACGAGTTC+CGG | 0.462931 | 5:+23914338 | MsG0580025769.01.T01:CDS |
| AAGTTGAGAAGTAACAGTGA+CGG | 0.682879 | 5:+23914284 | MsG0580025769.01.T01:CDS |
| ACGAGTTCTGCGGCGGTGGA+AGG | 0.479410 | 5:+23914179 | MsG0580025769.01.T01:CDS |
| AGATTCATTATCGTAATCGT+CGG | 0.618554 | 5:-23914096 | None:intergenic |
| AGCAACATTCTTCGTCTCGC+CGG | 0.532365 | 5:-23914363 | None:intergenic |
| AGCCACGAGTTCTGCGGCGG+TGG | 0.451550 | 5:+23914175 | MsG0580025769.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGAAAGTGAAAATGGTGAA+AGG | + | Chr5:23914389-23914408 | MsG0580025769.01.T01:CDS | 30.0% | |
| AGATTCATTATCGTAATCGT+CGG | - | Chr5:23914099-23914118 | None:intergenic | 30.0% | |
| CATATAGGCAAGAATTGTTT+GGG | + | Chr5:23914504-23914523 | MsG0580025769.01.T01:CDS | 30.0% | |
| GAAAATGGTGAAAGGTAAAA+AGG | + | Chr5:23914397-23914416 | MsG0580025769.01.T01:CDS | 30.0% | |
| TCGACGAAAAATTTCTTTAC+CGG | - | Chr5:23914134-23914153 | None:intergenic | 30.0% | |
| TGACCGAAAAAAGGAAAAAT+CGG | - | Chr5:23914062-23914081 | None:intergenic | 30.0% | |
| TGCTGTAAAGAAAGTGAAAA+TGG | + | Chr5:23914382-23914401 | MsG0580025769.01.T01:CDS | 30.0% | |
| AAAATTTCTTTACCGGCGAA+CGG | - | Chr5:23914127-23914146 | None:intergenic | 35.0% | |
| AAGTTGAGAAGTAACAGTGA+CGG | + | Chr5:23914284-23914303 | MsG0580025769.01.T01:CDS | 35.0% | |
| AATGGTGAAAGGTAAAAAGG+CGG | + | Chr5:23914400-23914419 | MsG0580025769.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 23913834 | 23914580 | 23913834 | ID=MsG0580025769.01;Name=MsG0580025769.01 |
| Chr5 | mRNA | 23913834 | 23914580 | 23913834 | ID=MsG0580025769.01.T01;Parent=MsG0580025769.01;Name=MsG0580025769.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|248 |
| Chr5 | exon | 23913834 | 23914580 | 23913834 | ID=MsG0580025769.01.T01:exon:24313;Parent=MsG0580025769.01.T01 |
| Chr5 | CDS | 23913834 | 23914580 | 23913834 | ID=MsG0580025769.01.T01:cds;Parent=MsG0580025769.01.T01 |
| Gene Sequence |
>MsG0580025769.01.T01 ATGCAAACTGTTTCAGCTTCACCTCTTAGCGAGGTTTCTGGAAAATTCGAGAACGATTTCTTCTCAAAGCTTAAAATTGAAGACGAGAAAATCGAAAACGAAAACGAAGATAAACACGAGGTAGAAGAAGAAGATGAAGATGATGATGAGTTTACATTTGTTTTCGCAGATCCTAAAGGTTCACCGATTTCCGCTGACGATGTGTTTGATAAAGGTCAGATTCGTCCGATTTTTCCTTTTTTCGGTCAGGATCTTCATTTCACCGACGATTACGATAATGAATCTGGAAACCGTTCGCCGGTAAAGAAATTTTTCGTCGAATCACCGTCTTCGTCGAAGACAGCCACGAGTTCTGCGGCGGTGGAAGGGACGTACTGCGAGTGGAATCCAACAAAAGCGGTGAAGAGTAATTCAACAGGATCATCAAACCTATGGAAGTTAAGAGATCCGAAGTTGAGAAGTAACAGTGACGGAAAAGACGCGTTCGTGTTTTTGAATCCGGCGAAGGTAGAGAAAACGAGTTCCGGCGCCGGCGAGACGAAGAATGTTGCTGTAAAGAAAGTGAAAATGGTGAAAGGTAAAAAGGCGGCTCCGGCGCCGTCGGCGCATGAGAAACATTATGTGATGAGTAAAGCGAGGAAGGAAAGTGATAAACGGAAATCTTATTTGCCATATAGGCAAGAATTGTTTGGGTTCTTTGCTAGTACAAATGGATTGGGAATGAGTAGGAATGTTCATCCTTATTGA |
| Protein sequence |
>MsG0580025769.01.T01 MQTVSASPLSEVSGKFENDFFSKLKIEDEKIENENEDKHEVEEEDEDDDEFTFVFADPKGSPISADDVFDKGQIRPIFPFFGQDLHFTDDYDNESGNRSPVKKFFVESPSSSKTATSSAAVEGTYCEWNPTKAVKSNSTGSSNLWKLRDPKLRSNSDGKDAFVFLNPAKVEKTSSGAGETKNVAVKKVKMVKGKKAAPAPSAHEKHYVMSKARKESDKRKSYLPYRQELFGFFASTNGLGMSRNVHPY* |
