Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0080048656.01.T01 | RHN42763.1 | 96.296 | 108 | 4 | 0 | 1 | 108 | 1 | 108 | 2.72E-68 | 211 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0080048656.01.T01 | A0A396GQG2 | 96.296 | 108 | 4 | 0 | 1 | 108 | 1 | 108 | 1.30e-68 | 211 |
Gene ID | Type | Classification |
---|
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsG0080048197.01 | MsG0080048656.01 | 0.801174 | 1.019794e-48 | 4.837882e-46 |
MsG0080048656.01 | MsG0080048764.01 | 0.831207 | 1.903074e-55 | 2.050943e-52 |
MsG0080048656.01 | MsG0080048843.01 | 0.801649 | 8.149125e-49 | 3.912421e-46 |
MsG0080048656.01 | MsG0180000157.01 | 0.831833 | 1.334349e-55 | 1.465212e-52 |
MsG0080048656.01 | MsG0180000673.01 | 0.806554 | 7.775779e-50 | 4.233082e-47 |
MsG0080048656.01 | MsG0180000739.01 | 0.823605 | 1.265763e-53 | 1.095032e-50 |
MsG0080048656.01 | MsG0180001043.01 | 0.871775 | 5.285816e-67 | 2.158471e-63 |
MsG0080048656.01 | MsG0180001921.01 | 0.826670 | 2.388242e-54 | 2.254639e-51 |
MsG0080048656.01 | MsG0180002482.01 | 0.811651 | 6.293671e-51 | 3.917510e-48 |
MsG0080048656.01 | MsG0180002491.01 | 0.804233 | 2.383631e-49 | 1.222475e-46 |
MsG0080048656.01 | MsG0180003088.01 | 0.828465 | 8.856248e-55 | 8.809874e-52 |
MsG0080048656.01 | MsG0180003174.01 | 0.814979 | 1.169091e-51 | 7.959752e-49 |
MsG0080048656.01 | MsG0180004388.01 | 0.808943 | 2.415179e-50 | 1.399354e-47 |
MsG0080048656.01 | MsG0180004886.01 | -0.806605 | 7.586319e-50 | 4.135157e-47 |
MsG0080048656.01 | MsG0180005125.01 | 0.837611 | 4.693405e-57 | 6.129513e-54 |
MsG0080048656.01 | MsG0180005424.01 | 0.801037 | 1.087578e-48 | 5.141909e-46 |
MsG0080048656.01 | MsG0180006016.01 | 0.814878 | 1.231232e-51 | 8.359664e-49 |
MsG0080048656.01 | MsG0280006518.01 | 0.812003 | 5.274502e-51 | 3.314395e-48 |
MsG0080048656.01 | MsG0280006596.01 | 0.805624 | 1.219959e-49 | 6.485286e-47 |
MsG0080048656.01 | MsG0280007439.01 | 0.855651 | 5.468271e-62 | 1.271460e-58 |
MsG0080048656.01 | MsG0280007609.01 | 0.828837 | 7.201701e-55 | 7.240891e-52 |
MsG0080048656.01 | MsG0280008971.01 | 0.836936 | 6.986717e-57 | 8.937869e-54 |
MsG0080048656.01 | MsG0280009235.01 | 0.822229 | 2.649243e-53 | 2.204209e-50 |
MsG0080048656.01 | MsG0280010389.01 | 0.804420 | 2.178433e-49 | 1.122666e-46 |
MsG0080048656.01 | MsG0280011235.01 | 0.813954 | 1.970098e-51 | 1.304677e-48 |
MsG0080048656.01 | MsG0380011754.01 | 0.833612 | 4.826346e-56 | 5.586819e-53 |
MsG0080048656.01 | MsG0380012661.01 | -0.821084 | 4.873247e-53 | 3.926037e-50 |
MsG0080048656.01 | MsG0380014680.01 | 0.806848 | 6.740316e-50 | 3.697264e-47 |
MsG0080048656.01 | MsG0380015686.01 | 0.805673 | 1.191408e-49 | 6.341535e-47 |
MsG0080048656.01 | MsG0380015779.01 | 0.837678 | 4.511904e-57 | 5.905125e-54 |
MsG0080048656.01 | MsG0380016227.01 | 0.806936 | 6.457101e-50 | 3.549858e-47 |
MsG0080048656.01 | MsG0380016828.01 | -0.811958 | 5.395877e-51 | 3.386408e-48 |
MsG0080048656.01 | MsG0380017226.01 | 0.839879 | 1.216483e-57 | 1.702895e-54 |
MsG0080048656.01 | MsG0380017286.01 | 0.826962 | 2.033889e-54 | 1.936505e-51 |
MsG0080048656.01 | MsG0380017403.01 | 0.800312 | 1.529185e-48 | 7.097808e-46 |
MsG0080048656.01 | MsG0380017998.01 | 0.809628 | 1.722799e-50 | 1.016697e-47 |
MsG0080048656.01 | MsG0380018001.01 | 0.813941 | 1.984005e-51 | 1.313348e-48 |
MsG0080048656.01 | MsG0480018213.01 | 0.801596 | 8.355281e-49 | 4.005925e-46 |
MsG0080048656.01 | MsG0480018603.01 | 0.806049 | 9.933202e-50 | 5.338389e-47 |
MsG0080048656.01 | MsG0480018906.01 | 0.803189 | 3.926081e-49 | 1.960383e-46 |
MsG0080048656.01 | MsG0480018970.01 | 0.815226 | 1.030334e-51 | 7.062099e-49 |
MsG0080048656.01 | MsG0480019163.01 | -0.827976 | 1.161584e-54 | 1.139194e-51 |
MsG0080048656.01 | MsG0480020445.01 | 0.824907 | 6.259081e-54 | 5.619825e-51 |
MsG0080048656.01 | MsG0480020791.01 | 0.824051 | 9.949798e-54 | 8.716133e-51 |
MsG0080048656.01 | MsG0480021731.01 | 0.807466 | 4.985539e-50 | 2.779020e-47 |
MsG0080048656.01 | MsG0480022035.01 | 0.801264 | 9.774319e-49 | 4.647444e-46 |
MsG0080048656.01 | MsG0480022052.01 | -0.808917 | 2.446572e-50 | 1.416537e-47 |
MsG0080048656.01 | MsG0480022480.01 | 0.808469 | 3.050542e-50 | 1.745690e-47 |
MsG0080048656.01 | MsG0480022707.01 | 0.827013 | 1.977306e-54 | 1.885296e-51 |
MsG0080048656.01 | MsG0480022768.01 | 0.828027 | 1.129596e-54 | 1.109456e-51 |
MsG0080048656.01 | MsG0480023088.01 | 0.812259 | 4.638910e-51 | 2.935406e-48 |
MsG0080048656.01 | MsG0480023417.01 | 0.801160 | 1.026694e-48 | 4.868955e-46 |
MsG0080048656.01 | MsG0480023517.01 | 0.828926 | 6.852334e-55 | 6.907891e-52 |
MsG0080048656.01 | MsG0580024191.01 | 0.812726 | 3.666729e-51 | 2.349155e-48 |
MsG0080048656.01 | MsG0580024458.01 | -0.806367 | 8.516223e-50 | 4.613859e-47 |
MsG0080048656.01 | MsG0580025134.01 | 0.845652 | 3.552883e-59 | 5.962438e-56 |
MsG0080048656.01 | MsG0580025278.01 | 0.805280 | 1.440377e-49 | 7.589260e-47 |
MsG0080048656.01 | MsG0580025328.01 | 0.802209 | 6.253356e-49 | 3.045301e-46 |
MsG0080048656.01 | MsG0580025769.01 | 0.817878 | 2.623574e-52 | 1.933850e-49 |
MsG0080048656.01 | MsG0580027034.01 | 0.837598 | 4.728451e-57 | 6.172875e-54 |
MsG0080048656.01 | MsG0580027345.01 | 0.805113 | 1.561227e-49 | 8.191045e-47 |
MsG0080048656.01 | MsG0580028185.01 | 0.829818 | 4.161206e-55 | 4.307190e-52 |
MsG0080048656.01 | MsG0580029148.01 | 0.805444 | 1.331340e-49 | 7.044472e-47 |
MsG0080048656.01 | MsG0580029189.01 | 0.804484 | 2.112669e-49 | 1.090566e-46 |
MsG0080048656.01 | MsG0580029258.01 | 0.826531 | 2.577086e-54 | 2.423240e-51 |
MsG0080048656.01 | MsG0580030235.01 | 0.813909 | 2.015973e-51 | 1.333402e-48 |
MsG0080048656.01 | MsG0680030685.01 | 0.808294 | 3.324459e-50 | 1.893690e-47 |
MsG0080048656.01 | MsG0680030841.01 | 0.805806 | 1.117524e-49 | 5.968563e-47 |
MsG0080048656.01 | MsG0680031538.01 | 0.829208 | 5.853339e-55 | 5.951115e-52 |
MsG0080048656.01 | MsG0680031694.01 | 0.850273 | 1.888666e-60 | 3.679674e-57 |
MsG0080048656.01 | MsG0680031712.01 | 0.814414 | 1.559460e-51 | 1.045778e-48 |
MsG0080048656.01 | MsG0680032430.01 | 0.811518 | 6.725270e-51 | 4.171954e-48 |
MsG0080048656.01 | MsG0680034450.01 | 0.828005 | 1.143151e-54 | 1.122070e-51 |
MsG0080048656.01 | MsG0680034672.01 | 0.810461 | 1.139427e-50 | 6.872394e-48 |
MsG0080048656.01 | MsG0780036291.01 | 0.829976 | 3.808576e-55 | 3.959413e-52 |
MsG0080048656.01 | MsG0780036550.01 | 0.809639 | 1.712772e-50 | 1.011094e-47 |
MsG0080048656.01 | MsG0780037503.01 | 0.801147 | 1.032891e-48 | 4.896724e-46 |
MsG0080048656.01 | MsG0780038444.01 | 0.834997 | 2.167857e-56 | 2.616155e-53 |
MsG0080048656.01 | MsG0780038495.01 | 0.812391 | 4.340485e-51 | 2.756392e-48 |
MsG0080048656.01 | MsG0780039841.01 | 0.803686 | 3.096503e-49 | 1.566010e-46 |
MsG0080048656.01 | MsG0780040726.01 | 0.840029 | 1.111846e-57 | 1.564028e-54 |
MsG0080048656.01 | MsG0780040861.01 | 0.834669 | 2.622980e-56 | 3.133989e-53 |
MsG0080048656.01 | MsG0780040929.01 | 0.843983 | 1.001330e-58 | 1.593715e-55 |
MsG0080048656.01 | MsG0780041399.01 | 0.808078 | 3.694956e-50 | 2.092771e-47 |
MsG0080048656.01 | MsG0880041969.01 | 0.823935 | 1.059256e-53 | 9.249966e-51 |
MsG0080048656.01 | MsG0880042611.01 | 0.809870 | 1.528235e-50 | 9.075898e-48 |
MsG0080048656.01 | MsG0880042811.01 | 0.811205 | 7.866797e-51 | 4.839659e-48 |
MsG0080048656.01 | MsG0880042843.01 | 0.805101 | 1.570352e-49 | 8.236400e-47 |
MsG0080048656.01 | MsG0880043002.01 | 0.810552 | 1.089310e-50 | 6.586047e-48 |
MsG0080048656.01 | MsG0880043082.01 | 0.814378 | 1.588729e-51 | 1.064312e-48 |
MsG0080048656.01 | MsG0880043303.01 | 0.801261 | 9.787085e-49 | 4.653189e-46 |
MsG0080048656.01 | MsG0880043456.01 | 0.810032 | 1.410215e-50 | 8.411008e-48 |
MsG0080048656.01 | MsG0880043465.01 | 0.802075 | 6.663026e-49 | 3.233844e-46 |
MsG0080048656.01 | MsG0880044415.01 | 0.805395 | 1.362624e-49 | 7.200996e-47 |
MsG0080048656.01 | MsG0880045583.01 | 0.817137 | 3.853707e-52 | 2.783471e-49 |
MsG0080048656.01 | MsG0880045631.01 | 0.820002 | 8.633158e-53 | 6.749046e-50 |
MsG0080048656.01 | MsG0880045803.01 | 0.800677 | 1.288317e-48 | 6.035254e-46 |
MsG0080048656.01 | MsG0880046058.01 | 0.833552 | 4.994480e-56 | 5.771598e-53 |
MsG0080048656.01 | MsG0880046091.01 | 0.800870 | 1.176762e-48 | 5.539783e-46 |
MsG0080048656.01 | MsG0880046731.01 | 0.845655 | 3.546893e-59 | 5.953046e-56 |
MsG0080048656.01 | MsG0880046733.01 | 0.819285 | 1.258486e-52 | 9.642773e-50 |
MsG0080048656.01 | MsG0880047191.01 | 0.806489 | 8.023882e-50 | 4.360842e-47 |
MsG0080048656.01 | MsG0880047590.01 | 0.805200 | 1.497182e-49 | 7.872696e-47 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0080048656.01.T01 | MTR_8g088460 | 94.495 | 109 | 6 | 0 | 1 | 109 | 1 | 109 | 9.93e-72 | 210 |
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0080048656.01.T01 | AT1G55365 | 49.275 | 69 | 33 | 1 | 1 | 67 | 1 | 69 | 6.26e-16 | 68.6 |
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
TCTGATGATGCTTCTGCTTC+TGG | 0.168743 | contig393end:+10201 | MsG0080048656.01.T01:CDS |
AAAAGCTGATCACTGTTTAT+TGG | 0.179925 | contig393end:-10045 | None:intergenic |
AAGAAGATAAGAAGAGTAAA+AGG | 0.234240 | contig393end:-10154 | None:intergenic |
AATAAACAGTGATCAGCTTT+TGG | 0.289636 | contig393end:+10047 | MsG0080048656.01.T01:CDS |
TAAGAAGAGTAAAAGGAATA+AGG | 0.294983 | contig393end:-10147 | None:intergenic |
TGTTTCCCTCTTCATCAACT+TGG | 0.458525 | contig393end:+10081 | MsG0080048656.01.T01:CDS |
GAGTAAAAGGAATAAGGTTG+AGG | 0.501247 | contig393end:-10141 | None:intergenic |
AGACGACCAAGTTGATGAAG+AGG | 0.585076 | contig393end:-10087 | None:intergenic |
GACGACCAAGTTGATGAAGA+GGG | 0.605275 | contig393end:-10086 | None:intergenic |
TGTCTGCACAAACGTGGCAA+TGG | 0.611212 | contig393end:+10004 | MsG0080048656.01.T01:CDS |
GAAAATGGTGTTGAACACTC+AGG | 0.614987 | contig393end:+9960 | None:intergenic |
CACGTGTGTCTGCACAAACG+TGG | 0.716541 | contig393end:+9998 | MsG0080048656.01.T01:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
! | AAGAAGATAAGAAGAGTAAA+AGG | - | contig393end:10157-10176 | None:intergenic | 25.0% |
! | TAAGAAGAGTAAAAGGAATA+AGG | - | contig393end:10150-10169 | None:intergenic | 25.0% |
AAAAGCTGATCACTGTTTAT+TGG | - | contig393end:10048-10067 | None:intergenic | 30.0% | |
! | AATAAACAGTGATCAGCTTT+TGG | + | contig393end:10047-10066 | MsG0080048656.01.T01:CDS | 30.0% |
GAGTAAAAGGAATAAGGTTG+AGG | - | contig393end:10144-10163 | None:intergenic | 35.0% | |
TGTTTCCCTCTTCATCAACT+TGG | + | contig393end:10081-10100 | MsG0080048656.01.T01:CDS | 40.0% | |
! | TCTGATGATGCTTCTGCTTC+TGG | + | contig393end:10201-10220 | MsG0080048656.01.T01:CDS | 45.0% |
!! | AGACGACCAAGTTGATGAAG+AGG | - | contig393end:10090-10109 | None:intergenic | 45.0% |
!! | GACGACCAAGTTGATGAAGA+GGG | - | contig393end:10089-10108 | None:intergenic | 45.0% |
TGTCTGCACAAACGTGGCAA+TGG | + | contig393end:10004-10023 | MsG0080048656.01.T01:CDS | 50.0% | |
CACGTGTGTCTGCACAAACG+TGG | + | contig393end:9998-10017 | MsG0080048656.01.T01:CDS | 55.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
contig393end | gene | 9964 | 10308 | 9964 | ID=MsG0080048656.01;Name=MsG0080048656.01 |
contig393end | mRNA | 9964 | 10308 | 9964 | ID=MsG0080048656.01.T01;Parent=MsG0080048656.01;Name=MsG0080048656.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|114 |
contig393end | exon | 9964 | 10308 | 9964 | ID=MsG0080048656.01.T01:exon:9213;Parent=MsG0080048656.01.T01 |
contig393end | CDS | 9964 | 10308 | 9964 | ID=MsG0080048656.01.T01:cds;Parent=MsG0080048656.01.T01 |
Gene Sequence |
Protein sequence |