AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048656.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048656.01.T01 MTR_8g088460 94.495 109 6 0 1 109 1 109 9.93e-72 210
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048656.01.T01 AT1G55365 49.275 69 33 1 1 67 1 69 6.26e-16 68.6

Find 12 sgRNAs with CRISPR-Local

Find 11 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGATGATGCTTCTGCTTC+TGG 0.168743 contig393end:+10201 MsG0080048656.01.T01:CDS
AAAAGCTGATCACTGTTTAT+TGG 0.179925 contig393end:-10045 None:intergenic
AAGAAGATAAGAAGAGTAAA+AGG 0.234240 contig393end:-10154 None:intergenic
AATAAACAGTGATCAGCTTT+TGG 0.289636 contig393end:+10047 MsG0080048656.01.T01:CDS
TAAGAAGAGTAAAAGGAATA+AGG 0.294983 contig393end:-10147 None:intergenic
TGTTTCCCTCTTCATCAACT+TGG 0.458525 contig393end:+10081 MsG0080048656.01.T01:CDS
GAGTAAAAGGAATAAGGTTG+AGG 0.501247 contig393end:-10141 None:intergenic
AGACGACCAAGTTGATGAAG+AGG 0.585076 contig393end:-10087 None:intergenic
GACGACCAAGTTGATGAAGA+GGG 0.605275 contig393end:-10086 None:intergenic
TGTCTGCACAAACGTGGCAA+TGG 0.611212 contig393end:+10004 MsG0080048656.01.T01:CDS
GAAAATGGTGTTGAACACTC+AGG 0.614987 contig393end:+9960 None:intergenic
CACGTGTGTCTGCACAAACG+TGG 0.716541 contig393end:+9998 MsG0080048656.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAGAAGATAAGAAGAGTAAA+AGG - contig393end:10157-10176 None:intergenic 25.0%
! TAAGAAGAGTAAAAGGAATA+AGG - contig393end:10150-10169 None:intergenic 25.0%
AAAAGCTGATCACTGTTTAT+TGG - contig393end:10048-10067 None:intergenic 30.0%
! AATAAACAGTGATCAGCTTT+TGG + contig393end:10047-10066 MsG0080048656.01.T01:CDS 30.0%
GAGTAAAAGGAATAAGGTTG+AGG - contig393end:10144-10163 None:intergenic 35.0%
TGTTTCCCTCTTCATCAACT+TGG + contig393end:10081-10100 MsG0080048656.01.T01:CDS 40.0%
! TCTGATGATGCTTCTGCTTC+TGG + contig393end:10201-10220 MsG0080048656.01.T01:CDS 45.0%
!! AGACGACCAAGTTGATGAAG+AGG - contig393end:10090-10109 None:intergenic 45.0%
!! GACGACCAAGTTGATGAAGA+GGG - contig393end:10089-10108 None:intergenic 45.0%
TGTCTGCACAAACGTGGCAA+TGG + contig393end:10004-10023 MsG0080048656.01.T01:CDS 50.0%
CACGTGTGTCTGCACAAACG+TGG + contig393end:9998-10017 MsG0080048656.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
contig393end gene 9964 10308 9964 ID=MsG0080048656.01;Name=MsG0080048656.01
contig393end mRNA 9964 10308 9964 ID=MsG0080048656.01.T01;Parent=MsG0080048656.01;Name=MsG0080048656.01.T01;_AED=0.45;_eAED=0.45;_QI=0|-1|0|1|-1|0|1|0|114
contig393end exon 9964 10308 9964 ID=MsG0080048656.01.T01:exon:9213;Parent=MsG0080048656.01.T01
contig393end CDS 9964 10308 9964 ID=MsG0080048656.01.T01:cds;Parent=MsG0080048656.01.T01
Gene Sequence

>MsG0080048656.01.T01

ATGGTGTTGAACACTCAGGTTGTTGTTCGAGTTGCACGTGTGTCTGCACAAACGTGGCAATGGTTGTCGTGCATTCATGATCCAATAAACAGTGATCAGCTTTTGGATCTTCTTTTTTGTTTCCCTCTTCATCAACTTGGTCGTCTTACTCTTTGTCTTTGTTCTTTCTTCTGTCTTCCTCAACCTTATTCCTTTTACTCTTCTTATCTTCTTTCTGATTCTGAGTTTGATTCTGATTCTGATGATGCTTCTGCTTCTGGTTCTTCAACTTCTACTCTTCATCTTGAACATGATTATTATTATCATTCTCATTCTGATTGTAAGTTTTTCTTCATCTTTTTTTAA

Protein sequence

>MsG0080048656.01.T01

MVLNTQVVVRVARVSAQTWQWLSCIHDPINSDQLLDLLFCFPLHQLGRLTLCLCSFFCLPQPYSFYSSYLLSDSEFDSDSDDASASGSSTSTLHLEHDYYYHSHSDCKFFFIFF*