AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000157.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 4 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCGTAAGATTGTTGTGAAGA+TGG 0.478593 1:-2162398 MsG0180000157.01.T01:CDS
GGTTCTATGAAGAAAGAAGA+AGG 0.541223 1:-2162331 MsG0180000157.01.T01:CDS
TCACAACAACAAGAAGAAGA+AGG 0.587622 1:-2162352 MsG0180000157.01.T01:CDS
ATCTTCACAACAATCTTACG+AGG 0.649549 1:+2162400 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAAAAACAAAGGAAATTT+GGG - Chr1:2162323-2162342 MsG0180000157.01.T01:CDS 20.0%
! CACAAAAAACAAAGGAAATT+TGG - Chr1:2162322-2162341 MsG0180000157.01.T01:CDS 25.0%
! TAAGAAACCACAAAAAACAA+AGG - Chr1:2162314-2162333 MsG0180000157.01.T01:CDS 25.0%
!!! CAAATTTCCTTTGTTTTTTG+TGG + Chr1:2162324-2162343 None:intergenic 25.0%
ATCTTCACAACAATCTTACG+AGG + Chr1:2162423-2162442 None:intergenic 35.0%
GGTTCTATGAAGAAAGAAGA+AGG - Chr1:2162489-2162508 MsG0180000157.01.T01:CDS 35.0%
TCACAACAACAAGAAGAAGA+AGG - Chr1:2162468-2162487 MsG0180000157.01.T01:CDS 35.0%
TCGTAAGATTGTTGTGAAGA+TGG - Chr1:2162422-2162441 MsG0180000157.01.T01:CDS 35.0%
CCTCAACCTACCAGAAAAAG+AGG - Chr1:2162393-2162412 MsG0180000157.01.T01:CDS 45.0%
! ACGAGGTCTACCTCTTTTTC+TGG + Chr1:2162406-2162425 None:intergenic 45.0%
!! CCTCTTTTTCTGGTAGGTTG+AGG + Chr1:2162396-2162415 None:intergenic 45.0%
!! GGTCTACCTCTTTTTCTGGT+AGG + Chr1:2162402-2162421 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr1 gene 2162309 2162533 2162309 ID=MsG0180000157.01;Name=MsG0180000157.01
Chr1 mRNA 2162309 2162533 2162309 ID=MsG0180000157.01.T01;Parent=MsG0180000157.01;Name=MsG0180000157.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|74
Chr1 exon 2162309 2162533 2162309 ID=MsG0180000157.01.T01:exon:6757;Parent=MsG0180000157.01.T01
Chr1 CDS 2162309 2162533 2162309 ID=MsG0180000157.01.T01:cds;Parent=MsG0180000157.01.T01
Gene Sequence

>MsG0180000157.01.T01

ATGGGTAAGAAACCACAAAAAACAAAGGAAATTTGGGCAGCAATAGCTGAAGCATCATCAACAATAGATGAAAATCAAGAACAACCTCAACCTACCAGAAAAAGAGGTAGACCTCGTAAGATTGTTGTGAAGATGGAAAGTTCAGAAGAAAAAGCAGCTTCACAACAACAAGAAGAAGAAGGTTCTATGAAGAAAGAAGAAGGAACAAAAACATATCAGAAGTAG

Protein sequence

>MsG0180000157.01.T01

MGKKPQKTKEIWAAIAEASSTIDENQEQPQPTRKRGRPRKIVVKMESSEEKAASQQQEEEGSMKKEEGTKTYQK*