Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026308.01.T01 | PNX73788.1 | 53.659 | 123 | 25 | 1 | 6 | 128 | 215 | 305 | 6.50E-26 | 109 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000983.01 | MsG0580026308.01 | 0.837040 | 6.572864e-57 | 8.434693e-54 |
| MsG0180004099.01 | MsG0580026308.01 | 0.843132 | 1.691410e-58 | 2.620595e-55 |
| MsG0280010907.01 | MsG0580026308.01 | 0.805155 | 1.529854e-49 | 8.035273e-47 |
| MsG0580024506.01 | MsG0580026308.01 | 0.802232 | 6.184469e-49 | 3.013608e-46 |
| MsG0380015805.01 | MsG0580026308.01 | 0.809222 | 2.105693e-50 | 1.229231e-47 |
| MsG0580026308.01 | MsG0580026309.01 | 0.867488 | 1.319209e-65 | 4.614271e-62 |
| MsG0580026308.01 | MsG0580029496.01 | 0.814230 | 1.712506e-51 | 1.142608e-48 |
| MsG0580026308.01 | MsG0580029825.01 | 0.834218 | 3.403832e-56 | 4.012622e-53 |
| MsG0580026308.01 | MsG0680034890.01 | 0.819571 | 1.083170e-52 | 8.366582e-50 |
| MsG0580026308.01 | MsG0880041896.01 | 0.840975 | 6.288872e-58 | 9.108443e-55 |
| MsG0280008909.01 | MsG0580026308.01 | 0.819688 | 1.018680e-52 | 7.893703e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580026308.01.T01 | MTR_5g043690 | 53.659 | 123 | 25 | 1 | 6 | 128 | 223 | 313 | 1.03e-29 | 109 |
| MsG0580026308.01.T01 | MTR_5g043690 | 53.659 | 123 | 25 | 1 | 6 | 128 | 222 | 312 | 1.06e-29 | 109 |
| MsG0580026308.01.T01 | MTR_5g043685 | 57.377 | 122 | 37 | 4 | 6 | 118 | 156 | 271 | 3.78e-25 | 96.7 |
| MsG0580026308.01.T01 | MTR_4g082910 | 49.153 | 59 | 30 | 0 | 70 | 128 | 298 | 356 | 3.94e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTACATACTTAATCTTATT+TGG | 0.119177 | 5:+34459320 | None:intergenic |
| TGAAGAAAGGTTGAAATCTT+TGG | 0.219285 | 5:-34459351 | MsG0580026308.01.T01:CDS |
| TTCCTTCACTAGCTTGCGTT+TGG | 0.377600 | 5:+34459708 | None:intergenic |
| CGAAAGAGCGGATAGATTCC+TGG | 0.384743 | 5:-34459753 | MsG0580026308.01.T01:CDS |
| GCAAGTACAATTTCAACAAC+AGG | 0.459062 | 5:-34459125 | MsG0580026308.01.T01:CDS |
| CTTCCTCTTCTTGTCTACCC+AGG | 0.480371 | 5:+34459735 | None:intergenic |
| TATGTAAATCTAGTTGCAAA+AGG | 0.492757 | 5:-34459206 | MsG0580026308.01.T01:CDS |
| TGCAGAGTGAACTTGAAGAA+AGG | 0.496563 | 5:-34459364 | MsG0580026308.01.T01:intron |
| ACGCAAGCTAGTGAAGGAAC+AGG | 0.521158 | 5:-34459704 | MsG0580026308.01.T01:intron |
| AATTGCAGAGGAAAATTGAT+CGG | 0.551351 | 5:-34459271 | MsG0580026308.01.T01:CDS |
| GGCAAAAGTTGAAATCTAAG+AGG | 0.565242 | 5:-34459250 | MsG0580026308.01.T01:CDS |
| ATTCCTGGGTAGACAAGAAG+AGG | 0.566055 | 5:-34459738 | MsG0580026308.01.T01:CDS |
| TCGCTAATTGCGAAACATCA+AGG | 0.566964 | 5:-34459069 | MsG0580026308.01.T01:three_prime_UTR |
| GAAAGAGCGGATAGATTCCT+GGG | 0.571450 | 5:-34459752 | MsG0580026308.01.T01:CDS |
| AGGAGCGAGAGACAAGGCTG+AGG | 0.595200 | 5:-34459186 | MsG0580026308.01.T01:CDS |
| AGCCAAACGCAAGCTAGTGA+AGG | 0.599861 | 5:-34459710 | MsG0580026308.01.T01:CDS |
| GAGCAAATTTCAAATTGCAG+AGG | 0.614710 | 5:-34459283 | MsG0580026308.01.T01:CDS |
| AGAGCGACAAGCAAAAGAGA+AGG | 0.623402 | 5:-34459147 | MsG0580026308.01.T01:CDS |
| TATGTAAAACTAGTTGCAGA+CGG | 0.645184 | 5:-34459305 | MsG0580026308.01.T01:CDS |
| TGCAAAAGGAGCGAGAGACA+AGG | 0.645612 | 5:-34459192 | MsG0580026308.01.T01:CDS |
| CAGAAGCAGAAATACGTCTG+TGG | 0.688002 | 5:+34459095 | None:intergenic |
| GGTATGAGAAGTCGAAAGAG+CGG | 0.693587 | 5:-34459765 | MsG0580026308.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAAACTAGAG+AGG | - | Chr5:34459289-34459308 | MsG0580026308.01.T01:CDS | 15.0% |
| !! | TTTACATACTTAATCTTATT+TGG | + | Chr5:34459429-34459448 | None:intergenic | 15.0% |
| ! | GAATAAGACACAAATTTAAC+AGG | + | Chr5:34459175-34459194 | None:intergenic | 25.0% |
| ! | TATGTAAATCTAGTTGCAAA+AGG | - | Chr5:34459540-34459559 | MsG0580026308.01.T01:intron | 25.0% |
| AAAAAAAAAACTAGAGAGGC+CGG | - | Chr5:34459293-34459312 | MsG0580026308.01.T01:CDS | 30.0% | |
| AATTGCAGAGGAAAATTGAT+CGG | - | Chr5:34459475-34459494 | MsG0580026308.01.T01:intron | 30.0% | |
| CATTTAATCCTTCTTTAAGC+AGG | - | Chr5:34459106-34459125 | MsG0580026308.01.T01:CDS | 30.0% | |
| CTAAAATCAGATCATGTTCT+AGG | - | Chr5:34459148-34459167 | MsG0580026308.01.T01:CDS | 30.0% | |
| TATGTAAAACTAGTTGCAGA+CGG | - | Chr5:34459441-34459460 | MsG0580026308.01.T01:intron | 30.0% | |
| TCTAGTATGAACAATAAGTC+TGG | - | Chr5:34459256-34459275 | MsG0580026308.01.T01:CDS | 30.0% | |
| TGAAGAAAGGTTGAAATCTT+TGG | - | Chr5:34459395-34459414 | MsG0580026308.01.T01:intron | 30.0% | |
| !!! | ACATGATCTGATTTTAGCTA+AGG | + | Chr5:34459145-34459164 | None:intergenic | 30.0% |
| AAAAAAAAACTAGAGAGGCC+GGG | - | Chr5:34459294-34459313 | MsG0580026308.01.T01:CDS | 35.0% | |
| GAGCAAATTTCAAATTGCAG+AGG | - | Chr5:34459463-34459482 | MsG0580026308.01.T01:intron | 35.0% | |
| GCAAGTACAATTTCAACAAC+AGG | - | Chr5:34459621-34459640 | MsG0580026308.01.T01:intron | 35.0% | |
| GGCAAAAGTTGAAATCTAAG+AGG | - | Chr5:34459496-34459515 | MsG0580026308.01.T01:intron | 35.0% | |
| TTTCATGACCTGCTTAAAGA+AGG | + | Chr5:34459117-34459136 | None:intergenic | 35.0% | |
| ! | TCTCTCAGACACTCTTTTTA+AGG | - | Chr5:34459067-34459086 | MsG0580026308.01.T01:three_prime_UTR | 35.0% |
| TCGCTAATTGCGAAACATCA+AGG | - | Chr5:34459677-34459696 | MsG0580026308.01.T01:intron | 40.0% | |
| TGCAGAGTGAACTTGAAGAA+AGG | - | Chr5:34459382-34459401 | MsG0580026308.01.T01:intron | 40.0% | |
| !!! | CTCCCACTACATTTTTTACC+CGG | + | Chr5:34459315-34459334 | None:intergenic | 40.0% |
| AGAGCGACAAGCAAAAGAGA+AGG | - | Chr5:34459599-34459618 | MsG0580026308.01.T01:intron | 45.0% | |
| AGGCCGGGTAAAAAATGTAG+TGG | - | Chr5:34459309-34459328 | MsG0580026308.01.T01:CDS | 45.0% | |
| ATTCCTGGGTAGACAAGAAG+AGG | - | Chr5:34459008-34459027 | MsG0580026308.01.T01:three_prime_UTR | 45.0% | |
| CAGAAGCAGAAATACGTCTG+TGG | + | Chr5:34459654-34459673 | None:intergenic | 45.0% | |
| GAAAGAGCGGATAGATTCCT+GGG | - | Chr5:34458994-34459013 | MsG0580026308.01.T01:three_prime_UTR | 45.0% | |
| GGCCGGGTAAAAAATGTAGT+GGG | - | Chr5:34459310-34459329 | MsG0580026308.01.T01:CDS | 45.0% | |
| GGTATGAGAAGTCGAAAGAG+CGG | - | Chr5:34458981-34459000 | MsG0580026308.01.T01:three_prime_UTR | 45.0% | |
| ! | TTCCTTCACTAGCTTGCGTT+TGG | + | Chr5:34459041-34459060 | None:intergenic | 45.0% |
| ACGCAAGCTAGTGAAGGAAC+AGG | - | Chr5:34459042-34459061 | MsG0580026308.01.T01:three_prime_UTR | 50.0% | |
| AGCCAAACGCAAGCTAGTGA+AGG | - | Chr5:34459036-34459055 | MsG0580026308.01.T01:three_prime_UTR | 50.0% | |
| CGAAAGAGCGGATAGATTCC+TGG | - | Chr5:34458993-34459012 | MsG0580026308.01.T01:three_prime_UTR | 50.0% | |
| CTTCCTCTTCTTGTCTACCC+AGG | + | Chr5:34459014-34459033 | None:intergenic | 50.0% | |
| TGCAAAAGGAGCGAGAGACA+AGG | - | Chr5:34459554-34459573 | MsG0580026308.01.T01:intron | 50.0% | |
| AGGAGCGAGAGACAAGGCTG+AGG | - | Chr5:34459560-34459579 | MsG0580026308.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 34458965 | 34459803 | 34458965 | ID=MsG0580026308.01;Name=MsG0580026308.01 |
| Chr5 | mRNA | 34458965 | 34459803 | 34458965 | ID=MsG0580026308.01.T01;Parent=MsG0580026308.01;Name=MsG0580026308.01.T01;_AED=0.42;_eAED=0.43;_QI=0|1|0.5|1|1|1|2|129|128 |
| Chr5 | exon | 34458965 | 34459381 | 34458965 | ID=MsG0580026308.01.T01:exon:13941;Parent=MsG0580026308.01.T01 |
| Chr5 | exon | 34459705 | 34459803 | 34459705 | ID=MsG0580026308.01.T01:exon:13940;Parent=MsG0580026308.01.T01 |
| Chr5 | CDS | 34459705 | 34459803 | 34459705 | ID=MsG0580026308.01.T01:cds;Parent=MsG0580026308.01.T01 |
| Chr5 | CDS | 34459094 | 34459381 | 34459094 | ID=MsG0580026308.01.T01:cds;Parent=MsG0580026308.01.T01 |
| Chr5 | three_prime_UTR | 34458965 | 34459093 | 34458965 | ID=MsG0580026308.01.T01:three_prime_utr;Parent=MsG0580026308.01.T01 |
| Gene Sequence |
| Protein sequence |
