AlfalfaGEDB | MsG0580027331.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027331.01

KEGG: K14638
GO: GO:0016020 GO:0022857 GO:0055085
PFAM: PF00854

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027331.01.T01	XP_003614522.2	96.111	540	19	2	1	539	1	539	0	1060

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027331.01.T01	Q9SX20	76.083	531	123	2	13	539	13	543	0	823

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027331.01.T01	A0A396HYQ0	96.111	540	19	2	1	539	1	539	0.0	1060

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048505.01	MsG0580027331.01	0.825891	3.660331e-54	3.379804e-51
MsG0080048845.01	MsG0580027331.01	0.826884	2.123331e-54	2.016919e-51
MsG0080048912.01	MsG0580027331.01	0.813367	2.652790e-51	1.729111e-48
MsG0080048938.01	MsG0580027331.01	0.817109	3.909543e-52	2.821573e-49
MsG0080048969.01	MsG0580027331.01	0.820050	8.418865e-53	6.590585e-50
MsG0080049130.01	MsG0580027331.01	0.821751	3.418881e-53	2.805939e-50
MsG0180000017.01	MsG0580027331.01	0.836064	1.164690e-56	1.451349e-53
MsG0180000784.01	MsG0580027331.01	0.812778	3.571733e-51	2.291305e-48
MsG0180000843.01	MsG0580027331.01	0.851175	1.053100e-60	2.113411e-57
MsG0180001100.01	MsG0580027331.01	0.828697	7.784755e-55	7.796079e-52

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PPI

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Gene1	Gene2	Type
MsG0180001738.01	MsG0580027331.01	PPI
MsG0180001746.01	MsG0580027331.01	PPI
MsG0180006251.01	MsG0580027331.01	PPI
MsG0480018595.01	MsG0580027331.01	PPI
MsG0480019554.01	MsG0580027331.01	PPI
MsG0480019555.01	MsG0580027331.01	PPI
MsG0580024502.01	MsG0580027331.01	PPI
MsG0580027331.01	MsG0480018595.01	PPI
MsG0580027331.01	MsG0480022856.01	PPI
MsG0580027331.01	MsG0380015545.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027331.01.T01	MTR_5g055000	96.082	536	19	2	5	539	1	535	0.0	1050
MsG0580027331.01.T01	MTR_7g088825	58.317	523	212	4	21	539	20	540	0.0	634
MsG0580027331.01.T01	MTR_7g088790	56.654	526	220	4	18	539	26	547	0.0	611
MsG0580027331.01.T01	MTR_7g088820	54.494	534	236	4	10	539	13	543	0.0	604
MsG0580027331.01.T01	MTR_7g088820	54.658	483	213	3	61	539	1	481	0.0	548
MsG0580027331.01.T01	MTR_7g088820	52.644	435	200	3	109	539	1	433	1.02e-165	479
MsG0580027331.01.T01	MTR_4g015080	44.465	533	282	10	11	539	24	546	8.86e-153	449
MsG0580027331.01.T01	MTR_7g067010	43.939	528	274	8	21	539	22	536	7.38e-152	447
MsG0580027331.01.T01	MTR_3g462710	42.505	527	295	7	16	539	17	538	2.92e-151	445
MsG0580027331.01.T01	MTR_3g112470	42.857	532	286	8	11	539	11	527	1.16e-143	426

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027331.01.T01	AT1G68570	76.083	531	123	2	13	539	13	543	0.0	823
MsG0580027331.01.T01	AT1G68570	76.817	509	114	2	35	539	5	513	0.0	799
MsG0580027331.01.T01	AT3G54140	43.925	535	290	7	8	539	11	538	2.63e-157	461
MsG0580027331.01.T01	AT3G54140	43.925	535	290	7	8	539	11	538	2.63e-157	461
MsG0580027331.01.T01	AT5G01180	44.423	529	271	8	21	539	23	538	6.04e-156	457
MsG0580027331.01.T01	AT5G01180	44.423	529	271	8	21	539	23	538	6.04e-156	457
MsG0580027331.01.T01	AT5G01180	44.423	529	271	8	21	539	23	538	6.04e-156	457
MsG0580027331.01.T01	AT2G02040	43.470	536	280	8	15	539	33	556	1.54e-149	441
MsG0580027331.01.T01	AT1G62200	43.019	530	284	7	16	539	45	562	9.31e-143	424
MsG0580027331.01.T01	AT1G62200	40.787	559	284	7	16	539	45	591	7.54e-138	413

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Find 159 sgRNAs with CRISPR-Local

Find 469 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCATTCGAAATCCCAGC+AGG	0.515566	5:+58538188	MsG0580027331.01.T01:CDS
AAATTCAGATCCATCATAAA+GGG	0.410856	5:+58538790	MsG0580027331.01.T01:three_prime_UTR
AACACTCTCACTAACTTTGG+TGG	0.517057	5:+58532621	MsG0580027331.01.T01:CDS
AACTGTTATCCCCTTTATGA+TGG	0.416802	5:-58538800	None:intergenic
AAGAAGGTAGCTATGGAGCA+CGG	0.668396	5:+58538398	MsG0580027331.01.T01:CDS
AAGACAATGTTGGATGGGGA+TGG	0.490096	5:+58534533	MsG0580027331.01.T01:CDS
AAGACCACCTCCTTGTAAAG+GGG	0.650880	5:+58534279	MsG0580027331.01.T01:CDS
AAGCTTCAGCCATTCCATGA+AGG	0.601801	5:-58538484	None:intergenic
AAGGGAGAAAGTGCCTTGTT+GGG	0.461179	5:-58538132	None:intergenic
AAGTTTACTGCAGGGCCTAA+TGG	0.441477	5:+58538641	MsG0580027331.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTCAGATCCATCATAAAG+GGG	+	Chr5:58538791-58538810	MsG0580027331.01.T01:three_prime_UTR	30.0%
AATTGTAACTCATGTTGTCA+TGG	+	Chr5:58532968-58532987	MsG0580027331.01.T01:intron	30.0%
AGCAAAAGAATCAGAAATGA+AGG	-	Chr5:58532676-58532695	None:intergenic	30.0%
AGTGATTAATTGTGCAAAGT+AGG	+	Chr5:58535941-58535960	MsG0580027331.01.T01:intron	30.0%
ATACTAGAAGGAAAAAAGGA+GGG	+	Chr5:58532129-58532148	MsG0580027331.01.T01:CDS	30.0%
ATATATTGTCCAAGCGATAA+AGG	+	Chr5:58536768-58536787	MsG0580027331.01.T01:intron	30.0%
ATATGAAAACACCAGACTTA+TGG	+	Chr5:58538015-58538034	MsG0580027331.01.T01:CDS	30.0%
ATATTCAAGACAATGTTGGA+TGG	+	Chr5:58534527-58534546	MsG0580027331.01.T01:CDS	30.0%
ATCTGATTCATCGAATTGAT+CGG	-	Chr5:58534416-58534435	None:intergenic	30.0%
ATGGTAGTAATCATACTAGA+AGG	+	Chr5:58532117-58532136	MsG0580027331.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	58532019	58539100	58532019	ID=MsG0580027331.01;Name=MsG0580027331.01
Chr5	mRNA	58532019	58539100	58532019	ID=MsG0580027331.01.T01;Parent=MsG0580027331.01;Name=MsG0580027331.01.T01;_AED=0.38;_eAED=0.38;_QI=55\|0.66\|0.75\|1\|1\|1\|4\|376\|541
Chr5	exon	58532019	58532176	58532019	ID=MsG0580027331.01.T01:exon:21904;Parent=MsG0580027331.01.T01
Chr5	exon	58532523	58532740	58532523	ID=MsG0580027331.01.T01:exon:21905;Parent=MsG0580027331.01.T01
Chr5	exon	58534238	58534794	58534238	ID=MsG0580027331.01.T01:exon:21906;Parent=MsG0580027331.01.T01
Chr5	exon	58537977	58539100	58537977	ID=MsG0580027331.01.T01:exon:21907;Parent=MsG0580027331.01.T01
Chr5	five_prime_UTR	58532019	58532073	58532019	ID=MsG0580027331.01.T01:five_prime_utr;Parent=MsG0580027331.01.T01
Chr5	CDS	58532074	58532176	58532074	ID=MsG0580027331.01.T01:cds;Parent=MsG0580027331.01.T01
Chr5	CDS	58532523	58532740	58532523	ID=MsG0580027331.01.T01:cds;Parent=MsG0580027331.01.T01
Chr5	CDS	58534238	58534794	58534238	ID=MsG0580027331.01.T01:cds;Parent=MsG0580027331.01.T01
Chr5	CDS	58537977	58538724	58537977	ID=MsG0580027331.01.T01:cds;Parent=MsG0580027331.01.T01
Chr5	three_prime_UTR	58538725	58539100	58538725	ID=MsG0580027331.01.T01:three_prime_utr;Parent=MsG0580027331.01.T01

Gene Sequence

>MsG0580027331.01.T01

ATGTACTGCAAAATGAATACAGGTTTTGAAAAGATGGAGAAGAATGGTAGTAATCATACTAGAAGGAAAAAAGGAGGGCTTGCAACAATGCCTTTTATCTTTGCTAATGAGGTTACTGAGAAGTTAGCAGTGGTGGGTTTCAATGCCAACATGATAAGCTACCTAACAACACAGCTTCACATGCCATTAACCAAAGCTGCTAACACTCTCACTAACTTTGGTGGAACTTCAAGCTTGACACCATTGCTTGGTGCCTTCATTTCTGATTCTTTTGCTGGAAAATTCATGACTATTACCGTTGCTTCCATTATTTACCAGATAGGTATGATTAGTTTGACTATGTCGGCGGTACTTCCACAATTAAGACCACCTCCTTGTAAAGGGGAGGAGGTATGCCAGGAAGCTAGTACAGGACAACTCGCTATTCTATATGTGTCACTTCTACTAAGTGCACTCGGATCGGGTGGGATCCGACCCTGCGTGGTAGCGTTTGGAGCCGATCAATTCGATGAATCAGATCCAAACCAAAAAACAAGGACATGGACTTATTTTAATTGGTACTATTTTGTCATGGGAACAGCTATACTTGTGGCTGTGACTGTTCTAGTTTATATTCAAGACAATGTTGGATGGGGATGGGGCCTTGGAATCCCCACATTGGCAATGTTCATCTCAATAATTGCATTCATTGGTGGATATCGACTTTACCGGAACTTGAACCCGGAAGGAAGCCCGTTTACCCGACTTGTAAAAGTGGGTGTTGCTGCTTTTCGTAAAAGGAAGATACCAAAAGTGCCTAACTCTACATTGTTATATCAAAATGATGAACTTGATGCATCAATAACATTAGGAGTAGGGATGTCAATGGGTACCCGCGGATTTTTAGACAAGGCAGCAATAGTGACAGAGGAAGACAATATGAAAACACCAGACTTATGGAGGCTATCAACTGTTCACAGAGTGGAGGAATTGAAATCAATAATCAGGATGGGACCAATATGGGCATCAGGCATTCTTCTAATCACAGCATATGCCCAACAAGGCACTTTCTCCCTTCAACAAGCCAAAACTATGAACAGACACCTAACCAAATCATTCGAAATCCCAGCAGGGTCCATGTCGGTATTCACCATCCTCACAATGCTCTTCACCATTGCCCTCTATGACCGTGTCTTAATCCGAGTGGCTCGTAGATTAACTGGACTTGACCGTGGTATAAGCTTCCTTCACAGAATGGGAATAGGGTTTGTAATCTCACTTTTTGCTACCTTTGTTGCTGGATTCGTCGAAATGAAACGAAAGAAGGTAGCTATGGAGCACGGACTCGTTGAACACTCTAGTGAAATAATCCCAATCTCAGTGTTTTGGCTTGTGCCACAATATAGCCTTCATGGAATGGCTGAAGCTTTTATGTCAATAGGGCATTTGGAGTTTTTCTATGATCAAGCTCCTGAGAGTATGACAAGTACTGCAATGGCATTTTTTTGGACATCAATTTCTCTAGGGAATTATATCAGTACACTTTTGGTTACCTTGGTACACAAGTTTACTGCAGGGCCTAATGGTACCAATTGGCTTCCTGATAATAATCTCAATAAGGGGAAGTTGGAATACTTTACTGGCTAA

Protein sequence

>MsG0580027331.01.T01

MYCKMNTGFEKMEKNGSNHTRRKKGGLATMPFIFANEVTEKLAVVGFNANMISYLTTQLHMPLTKAANTLTNFGGTSSLTPLLGAFISDSFAGKFMTITVASIIYQIGMISLTMSAVLPQLRPPPCKGEEVCQEASTGQLAILYVSLLLSALGSGGIRPCVVAFGADQFDESDPNQKTRTWTYFNWYYFVMGTAILVAVTVLVYIQDNVGWGWGLGIPTLAMFISIIAFIGGYRLYRNLNPEGSPFTRLVKVGVAAFRKRKIPKVPNSTLLYQNDELDASITLGVGMSMGTRGFLDKAAIVTEEDNMKTPDLWRLSTVHRVEELKSIIRMGPIWASGILLITAYAQQGTFSLQQAKTMNRHLTKSFEIPAGSMSVFTILTMLFTIALYDRVLIRVARRLTGLDRGISFLHRMGIGFVISLFATFVAGFVEMKRKKVAMEHGLVEHSSEIIPISVFWLVPQYSLHGMAEAFMSIGHLEFFYDQAPESMTSTAMAFFWTSISLGNYISTLLVTLVHKFTAGPNGTNWLPDNNLNKGKLEYFTG*