Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015545.01.T01 | XP_003601036.2 | 98.642 | 589 | 8 | 0 | 1 | 589 | 1 | 589 | 0 | 1201 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048068.01 | MsG0380015545.01 | 0.807212 | 5.642910e-50 | 3.124663e-47 |
| MsG0080048505.01 | MsG0380015545.01 | 0.836464 | 9.218160e-57 | 1.162595e-53 |
| MsG0080048801.01 | MsG0380015545.01 | 0.804918 | 1.714880e-49 | 8.953023e-47 |
| MsG0080048845.01 | MsG0380015545.01 | 0.862679 | 4.271533e-64 | 1.261465e-60 |
| MsG0080048969.01 | MsG0380015545.01 | 0.844174 | 8.903460e-59 | 1.425561e-55 |
| MsG0080049016.01 | MsG0380015545.01 | 0.820068 | 8.339420e-53 | 6.531770e-50 |
| MsG0080049040.01 | MsG0380015545.01 | 0.806338 | 8.636933e-50 | 4.675825e-47 |
| MsG0080049078.01 | MsG0380015545.01 | 0.829113 | 6.171649e-55 | 6.256525e-52 |
| MsG0080049079.01 | MsG0380015545.01 | 0.818275 | 2.133848e-52 | 1.590013e-49 |
| MsG0080049130.01 | MsG0380015545.01 | 0.853987 | 1.660856e-61 | 3.654788e-58 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015545.01.T01 | MTR_3g072300 | 98.642 | 589 | 8 | 0 | 1 | 589 | 1 | 589 | 0.0 | 1201 |
| MsG0380015545.01.T01 | MTR_7g098160 | 51.478 | 575 | 270 | 4 | 2 | 571 | 11 | 581 | 0.0 | 615 |
| MsG0380015545.01.T01 | MTR_7g098040 | 51.478 | 575 | 270 | 4 | 2 | 571 | 12 | 582 | 0.0 | 602 |
| MsG0380015545.01.T01 | MTR_1g041815 | 51.552 | 580 | 273 | 4 | 6 | 581 | 9 | 584 | 0.0 | 599 |
| MsG0380015545.01.T01 | MTR_1g075440 | 51.295 | 579 | 271 | 5 | 4 | 576 | 8 | 581 | 0.0 | 598 |
| MsG0380015545.01.T01 | MTR_7g098200 | 50.087 | 575 | 278 | 4 | 2 | 571 | 7 | 577 | 0.0 | 595 |
| MsG0380015545.01.T01 | MTR_7g098090 | 50.777 | 579 | 271 | 5 | 3 | 571 | 12 | 586 | 0.0 | 594 |
| MsG0380015545.01.T01 | MTR_1g041835 | 52.491 | 562 | 259 | 4 | 6 | 563 | 9 | 566 | 0.0 | 593 |
| MsG0380015545.01.T01 | MTR_7g098180 | 50.709 | 564 | 268 | 4 | 4 | 561 | 11 | 570 | 0.0 | 582 |
| MsG0380015545.01.T01 | MTR_7g098200 | 49.739 | 575 | 270 | 5 | 2 | 571 | 7 | 567 | 0.0 | 581 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015545.01.T01 | AT2G40460 | 72.014 | 586 | 155 | 3 | 1 | 584 | 1 | 579 | 0.0 | 860 |
| MsG0380015545.01.T01 | AT5G46050 | 48.881 | 581 | 285 | 6 | 2 | 576 | 5 | 579 | 0.0 | 547 |
| MsG0380015545.01.T01 | AT5G46040 | 49.315 | 584 | 280 | 10 | 2 | 578 | 5 | 579 | 0.0 | 533 |
| MsG0380015545.01.T01 | AT5G01180 | 45.167 | 569 | 304 | 6 | 1 | 564 | 1 | 566 | 0.0 | 532 |
| MsG0380015545.01.T01 | AT5G01180 | 45.167 | 569 | 304 | 6 | 1 | 564 | 1 | 566 | 0.0 | 532 |
| MsG0380015545.01.T01 | AT5G01180 | 45.167 | 569 | 304 | 6 | 1 | 564 | 1 | 566 | 0.0 | 532 |
| MsG0380015545.01.T01 | AT3G54140 | 44.561 | 570 | 310 | 4 | 1 | 566 | 1 | 568 | 1.90e-179 | 519 |
| MsG0380015545.01.T01 | AT3G54140 | 44.561 | 570 | 310 | 4 | 1 | 566 | 1 | 568 | 1.90e-179 | 519 |
| MsG0380015545.01.T01 | AT2G02040 | 42.908 | 564 | 315 | 5 | 7 | 565 | 24 | 585 | 1.80e-171 | 499 |
| MsG0380015545.01.T01 | AT1G62200 | 43.594 | 562 | 299 | 6 | 9 | 562 | 37 | 588 | 3.10e-167 | 489 |
Find 146 sgRNAs with CRISPR-Local
Find 308 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATCCCAGCAGCATCTCT+AGG | 0.522560 | 3:+71651684 | MsG0380015545.01.T01:CDS |
| AAACCAAATATCTCAACTTT+TGG | 0.301836 | 3:+71649162 | MsG0380015545.01.T01:CDS |
| AAAGAAGACTATACCCATGA+TGG | 0.538056 | 3:+71647938 | MsG0380015545.01.T01:CDS |
| AAATGGAACATTGTGTTCTT+AGG | 0.167140 | 3:+71649517 | MsG0380015545.01.T01:CDS |
| AACAACCAAAATATACTCAT+AGG | 0.391678 | 3:-71651933 | None:intergenic |
| AACAAGTTAATTAAGTAGAT+AGG | 0.342754 | 3:+71652503 | MsG0380015545.01.T01:three_prime_UTR |
| AAGATACTGTTTCATCAGTT+AGG | 0.396676 | 3:+71648241 | MsG0380015545.01.T01:CDS |
| AAGCCCTAAAGTTGCAATTA+AGG | 0.273762 | 3:-71649274 | None:intergenic |
| AAGCTGCCTAGAGATGCTGC+TGG | 0.575379 | 3:-71651690 | None:intergenic |
| AAGTAGATACATTTACAAGA+AGG | 0.524961 | 3:+71652253 | MsG0380015545.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAACAACCAAGCCTTAAAT+GGG | - | Chr3:71651146-71651165 | None:intergenic | 30.0% | |
| AAATCAAGTGCGTATCTATT+TGG | + | Chr3:71651236-71651255 | MsG0380015545.01.T01:intron | 30.0% | |
| AAATGGAACATTGTGTTCTT+AGG | + | Chr3:71649517-71649536 | MsG0380015545.01.T01:CDS | 30.0% | |
| AAATTGCTATGCTTACCAAT+TGG | + | Chr3:71648702-71648721 | MsG0380015545.01.T01:intron | 30.0% | |
| AAGATACTGTTTCATCAGTT+AGG | + | Chr3:71648241-71648260 | MsG0380015545.01.T01:CDS | 30.0% | |
| AATGGAACATTGTGTTCTTA+GGG | + | Chr3:71649518-71649537 | MsG0380015545.01.T01:CDS | 30.0% | |
| AATGGAAGGTAATTCAACTT+TGG | + | Chr3:71652310-71652329 | MsG0380015545.01.T01:CDS | 30.0% | |
| ACAATGACTGATAAGATAAC+AGG | + | Chr3:71652113-71652132 | MsG0380015545.01.T01:CDS | 30.0% | |
| ACTCTATTAAACTGTCAAGT+TGG | - | Chr3:71649848-71649867 | None:intergenic | 30.0% | |
| ACTCTTATGAATGATCACTT+TGG | + | Chr3:71648876-71648895 | MsG0380015545.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 71647831 | 71652614 | 71647831 | ID=MsG0380015545.01;Name=MsG0380015545.01 |
| Chr3 | mRNA | 71647831 | 71652614 | 71647831 | ID=MsG0380015545.01.T01;Parent=MsG0380015545.01;Name=MsG0380015545.01.T01;_AED=0.29;_eAED=0.29;_QI=98|1|1|1|1|1|4|256|589 |
| Chr3 | exon | 71647831 | 71648034 | 71647831 | ID=MsG0380015545.01.T01:exon:23822;Parent=MsG0380015545.01.T01 |
| Chr3 | exon | 71648163 | 71648380 | 71648163 | ID=MsG0380015545.01.T01:exon:23823;Parent=MsG0380015545.01.T01 |
| Chr3 | exon | 71649018 | 71649574 | 71649018 | ID=MsG0380015545.01.T01:exon:23824;Parent=MsG0380015545.01.T01 |
| Chr3 | exon | 71651470 | 71652614 | 71651470 | ID=MsG0380015545.01.T01:exon:23825;Parent=MsG0380015545.01.T01 |
| Chr3 | five_prime_UTR | 71647831 | 71647928 | 71647831 | ID=MsG0380015545.01.T01:five_prime_utr;Parent=MsG0380015545.01.T01 |
| Chr3 | CDS | 71647929 | 71648034 | 71647929 | ID=MsG0380015545.01.T01:cds;Parent=MsG0380015545.01.T01 |
| Chr3 | CDS | 71648163 | 71648380 | 71648163 | ID=MsG0380015545.01.T01:cds;Parent=MsG0380015545.01.T01 |
| Chr3 | CDS | 71649018 | 71649574 | 71649018 | ID=MsG0380015545.01.T01:cds;Parent=MsG0380015545.01.T01 |
| Chr3 | CDS | 71651470 | 71652358 | 71651470 | ID=MsG0380015545.01.T01:cds;Parent=MsG0380015545.01.T01 |
| Chr3 | three_prime_UTR | 71652359 | 71652614 | 71652359 | ID=MsG0380015545.01.T01:three_prime_utr;Parent=MsG0380015545.01.T01 |
| Gene Sequence |
>MsG0380015545.01.T01 ATGGAAGCCAAAGAAGACTATACCCATGATGGAACTGTTGATTTCCACGGCAAACCTGCTGTTCCATCCAAAACTGGGAAATGGAAGGCTTGTGCTTTTCTTGTTGGGTATGAAGCATTTGAAAGGATGGCCTTCTATGGAGTTGCATCAAACTTGGTGAATTACCTTACAACACAACTTCATGAAGATACTGTTTCATCAGTTAGGAATGTTAATACATGGTCAGGGTCTGTTTGGATAACACCAATTCTTGGTGCTTACATTGCAGATTCTTACCTGGGTCGCTTCTGGACTTTCACCCTCTCATCTCTCATTTATGTCTTGGGAATGACACTTCTTACTATAGCTGTATCACTCAAGAGCTTGAAACCAACATGCACAAATGGAATTTGCAACAAGGCTTCAACATCACAAATTACATTTTTCTACACAGCACTTTACACAATGGCAATTGGTGCAGGTGGAACAAAACCAAATATCTCAACTTTTGGTGCTGACCAATTTGATGATTTTAATCCTCATGAGAAAGAGTTAAAGGCTTCTTTCTTTAATTGGTGGATGTTTACCTCATTTTTAGGTGCCTTAATTGCAACTTTAGGGCTTGTATATATTCAAGAGAATTTAGGATGGGGACTTGGATATGGTATTCCTACTGCTGGTTTATTATTGTCTTTGGTTATTTTCTATATTGGGACACCAATTTATCGACACAAAGTTAGGACATCTAAAAGTCCTGCTAAGGATATAATTCGTGTCTTTATTGTTGCCTTCAAGAATAGAAAACTTCAACTTCCTAGTAACCCTTCAGATCTACATGAGTTTCAAATGGAACATTGTGTTCTTAGGGGAAAAAGGCAAGTCTATCACACTCCAACTTTGAGGTTTTTGGACAAAGCTGCTATTAAAGAAGATCCTACAACTGGTTCCTCAAGAAGAGCACCAATGACAGTAAATCAAGTAGAAGGAGCAAAGCTAATCCTTGGTATGCTCCTAATATGGTTAGTAACACTGATTCCAAGCACCATTTGGGCACAAATCAACACTCTTTTTGTGAAACAAGGCACTACCTTAGACAGAAACCTTGGTCCTGATTTTAAAATCCCAGCAGCATCTCTAGGCAGCTTTGTAACCCTATCCATGCTGCTCTCAGTGCCAATGTATGACCGGTTTTTCGTACCGTTCATGCGCCAGAAAACCGGCCACCCGAGAGGAATCACATTACTTCAAAGACTTGGAATAGGATTTTCAATCCAGATCATAGCCATTGCAATTGCTTATGCAGTAGAAGTTAGAAGAATGCATGTGATAAAAGAGAATCACATTTTTGGTCCTAAAGATATTGTCCCTATGAGTATATTTTGGTTGTTACCACAATATGTTCTTATTGGTATAGCAGATGTTTTCAATGCCATTGGATTATTGGAATTCTTCTATGATCAATCTCCTGAAGACATGCAAAGTCTTGGAACAACTTTCTTCACAAGTGGAATTGGTGTTGGGAATTTCTTGAACAGTTTTTTGGTAACAATGACTGATAAGATAACAGGAAGAGGGACTAGAAAGAGTTGGATTGCTGATAACTTGAATGATTCTCACTTAGATTACTATTATGGGTTTCTTTTGATCTTGTCAAGTGTTAATTTGTTGGTGTTTTTGTGGGTTTCAAGTAGATACATTTACAAGAAGGAGTCAACAAGAATCAAAGAAGCACTTTGTGTTCAAATGGAAGGTAATTCAACTTTGGATGCATCTCTTGGCTTACAAGTATGA |
| Protein sequence |
>MsG0380015545.01.T01 MEAKEDYTHDGTVDFHGKPAVPSKTGKWKACAFLVGYEAFERMAFYGVASNLVNYLTTQLHEDTVSSVRNVNTWSGSVWITPILGAYIADSYLGRFWTFTLSSLIYVLGMTLLTIAVSLKSLKPTCTNGICNKASTSQITFFYTALYTMAIGAGGTKPNISTFGADQFDDFNPHEKELKASFFNWWMFTSFLGALIATLGLVYIQENLGWGLGYGIPTAGLLLSLVIFYIGTPIYRHKVRTSKSPAKDIIRVFIVAFKNRKLQLPSNPSDLHEFQMEHCVLRGKRQVYHTPTLRFLDKAAIKEDPTTGSSRRAPMTVNQVEGAKLILGMLLIWLVTLIPSTIWAQINTLFVKQGTTLDRNLGPDFKIPAASLGSFVTLSMLLSVPMYDRFFVPFMRQKTGHPRGITLLQRLGIGFSIQIIAIAIAYAVEVRRMHVIKENHIFGPKDIVPMSIFWLLPQYVLIGIADVFNAIGLLEFFYDQSPEDMQSLGTTFFTSGIGVGNFLNSFLVTMTDKITGRGTRKSWIADNLNDSHLDYYYGFLLILSSVNLLVFLWVSSRYIYKKESTRIKEALCVQMEGNSTLDASLGLQV* |
